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Optima XE Series User Guide

1. A122 Alert Firmware error Firmware error caused reset Call Beckman Coulter Field Service D123 Ul Communications Communications error Call Beckman Coulter Field Service A124 Alert Hard drive space low Hard drive is 90 full Backup files soon Call Beckman Coulter Field Service D125 SBC Communications Communications parameters are not compatible Call Beckman Coulter Field Service A200 Alert AC Power Loss Run continued AC Power Loss Run continued None A201 Alert AC Power Loss Run stopped AC Power Loss Run Delayed Run or Program stopped None D202 Bus Current Bus current measures zero lt 0 1A Call Beckman Coulter Field Service D203 Bus Voltage Bus voltage too high gt 220VDC or Bus voltage too low lt 180VDC Call Beckman Coulter Field Service D204 Power Supply Inverter 18V supply too high gt 19 8VDC Call Beckman Coulter Field Service D205 Power Supply Inverter 18V supply too low lt 16 2VDC Call Beckman Coulter Field Service D206 Power Supply Inverter 5V supply too high gt 5 5VDC Call Beckman Coulter Field Service D207 Power Supply Inverter 5V supply too low lt 4 5VDC Call Beckman Coulter Field Service D208 Power Supply Inverter 5V supply too high gt 4 5VDC Call Beckman Coulter Field Service D209 Power Supply Inverter 5V
2. Can thermistor not connected Call Beckman Coulter Field Service C 5 Diagnostics Diagnostics User Messages Chart C 6 Table C 1 Diagnostics User Messages Chart Continued Message Definition Result Recommended Action D504 Temperature Control Can thermistor shorted Call Beckman Coulter Field Service D505 Temperature Control Can temperature out of limits lt 30 C or gt 70 C Call Beckman Coulter Field Service D506 Temperature Control TEM voltage too high Call Beckman Coulter Field Service D507 Temperature Control TEM voltage too low Call Beckman Coulter Field Service D508 Temperature Control TEM impedance too low Call Beckman Coulter Field Service D509 Temperature Control TEM impedance too high Call Beckman Coulter Field Service D510 Temperature Control Rotor temperature error rate of change is not decreasing and rotor temperature is greater than 10 C from set temperature after running for 15 minutes Call Beckman Coulter Field Service D512 Temperature Communications D512 Temperature Communications Call Beckman Coulter Field Service D513 Temperature Communications No communications TEM I2C Call Beckman Coulter Field Service D514 Temperature Communications No communications thermopile Call Beckman Coulter Field Service D600 DriveDrive fault Drive fa
3. e The Labware Details on the right side of the screen show all the details for the selected labware on the List of Labware e The Back Button returns you to the Rotor Catalog Page 4 26 Functional Pages 4 Zonal Operation Page Zonal Operation Page Figure 4 29 Zonal Operation Page Running _ Unloading starting E Stopping Press Start to go to Load speed Loading Complete Unioad Load Speed Vacuum Unload Speed Set Speed 50 000 RPM Set Time Hold The Zonal Operation Page appears when you enter the correct password and select the Authorize Button from the Zonal Authorization Page putting the instrument in Zonal mode When you use Zonal mode the Zonal Operation Page replaces the Home Page until Zonal mode ends In addition to the Header Bar and the Footer Bar the Zonal Operation Page contains the following elements e The Status Display shows the current step in the Zonal run procedure See Zonal and Continuous Flow Operation in the Operations chapter for details e The Loading Complete Button ends the sample loading step and proceeds to the running step The Unload Button terminates the running step early and proceeds to the sample unloading step e The Load Speed Display shows the sample loading speed Use the arrow buttons above and below to increase or decrease the sample loading speed e The Vacuum Display Button shows the current vacuum You press it to begin evacuating and precondition
4. Actual rotor temperature in 0 1 C increments e Ambient temperature range 10 to 35 C e Humidity restrictions lt 80 at lt 35 C non condensing e Condensation removal Automatic begins five minutes after vacuum vent e Vacuum Below 5 microns 0 7 Pa Ease of Use e Languages Multi language support e Help On screen context sensitive e Diagnostic messages 10 000 messages saved Physical Data Specification Description Dimensions e Width 94 0 cm 37 in Depth 68 1 cm 26 8 in e Height 125 7 cm 49 5 in e Weight 485 kg 1068 lb Ventilation Clearances e Sides 5 1cm 2 0 in e Rear 15 2 cm 6 0 in PN B04155AC 1 Description Specifications Specification Description Finishes e Rotor pad Coated polycarbonate Top and Front surfaces Polyurethane e Other surfaces Acrylic baking enamel Electrical e Power requirement 200 240 VAC 30A branch circuit 50 60 Hz Automatic tap selection on every run e Electrical supply Class e Power Consumption 60W in idle 1 0 kW average running in steady state at 90K rom e Installation overvoltage category il e LCD Monitor Wide tilt range horizontal and vertical Outputs e Power Factor 0 99 e Nominal Output Voltage 200 240 e Output Frequency 50 60
5. PN B04155AC The Touch Screen Interface J Help Messages Ifthe global help page is too large to fit in the page display area a Scroll Bar appears on the right side allowing you to scroll up and down through the page Figure 2 22 Zonal Continuous Flow Operation Help with scroll bar Zonal Continuous Flow Operation Page The ZonalContinuous Flow Operation Page appears when you enter the correct password and touch the Authorize Button from the ZonalContinuous Flow Authorization Page putting the instrument in Zonal mode or Continuous Flow mode When you use Zonal mode or Continuous Flow mode the Zonal Continuous Flow Operation Page replaces the Home Page until Zonal mode or Continuous Flow mode ends in addition to the Header Bar and the Footer Bar the Zonal Continuous Flow Operation Page contains the following elements The Status Display shows the current step in the Zonal Continuous Flow Run procedure See the Zonal Continuous Flow Run Procedure for details The Loading Complete Button ends the sample loading step and proceeds to the running step The Unload Button terminates the running step early and proceeds to the sample unloading step The Load Speed Display shows the sample loading speed Use the arrow buttons above and below to raise or lower the sample loading speed The Vacuum Display Button shows the current vacuum You press it to begin evacuating and preconditoning the Chamber at the beginning of a run o
6. The List of Labware on the left side of the screen shows the labware by volume type and part number Scroll through this list and select a particular labware to view the detailed information about it e The Labware Details on the right side of the screen shows all the details for the selected labware on the List of Labware e The Back Button returns you to the References Page PN B04155AC 4 19 Functional Pages Export User Manual Page Export User Manual Page Export User Manual Available Drives Insert USB drive Set Speed 15 000 RPM Set Tir 0 05 h mm Set Temp 25 C Selection of the Export User Manual Button allows you to copy the User Manual to a USB drive NOTE Do not remove the USB drive while a data transfer is in progress In addition to the Header Bar and the Footer Bar the Export User Manual Page contains the following elements e The Available Drives list shows all the available USB drives If the list is empty the prompt Insert USB Drive appears below the list area Select an available USB drive to receive the information The Back Button returns you to the previous page The Export Button transfers the information to the selected USB drive 4 20 PN BO4155AC Functional Pages 4 Set System Name Page Set System Name Page Set System Name Optima XE Set Speed 15 000 RPM Set Time 0 05 himm Set Temp 25 C The Set System Name Page appears when you select the Set System Name
7. Button on the System Options Page allowing you to set the System Name that appears in the Footer Bar on the Home Page In addition to the Header Bar and the Footer Bar the Set System Name Page contains the following elements e The Set System Name Display shows the current System Name e The Keyboard for entering the name It includes special keys for different character modes and corrections e The Cancel Button ignores any changes you have made and returns to the System Options Page e The OK Button accepts your changes and returns to the System Options Page The other keys work much like standard keyboard keys except that the Shift and Special Character Set keys work as toggle like a standard Caps Lock key There are also Clear and Backspace keys with symbolic markings PN B04155AC 4 21 Functional Pages Set Date and Time Page Set Date and Time Page Figure 4 24 Set Date and Time Page Set Date and Time Month Day Year Minute Ai es ha S55 Select Time Format 12 Hour e g 8 25 PM 24 Hour e g 20 25 eed 15 000 RPM Set Time 0 05 himm The Set Date and Time Page appears when you select the Set Date and Time Button on the System Options Page allowing you to set the instrument s internal time and date In addition to the Header Bar and the Footer Bar the Set Date and Time Page contains the following elements 4 22 The Month Day Year controls let you set the date Simply select th
8. Notices your work need not make them do so A compilation of a covered work with other separate and independent works which are not by their nature extensions of the covered work and which are not combined with it such as to form a larger program in or ona volume of a storage or distribution medium is called an aggregate if the compilation and its resulting copyright are not used to limit the access or legal rights of the compilation s users beyond which the individual works permit Inclusion of a covered work in an aggregate does not cause the License to apply to the other parts of the aggregate 6 Conveying Non Source Forms You may convey a covered work in object code form under the terms of sections 4 and 5 provided that you also convey the machine readable Corresponding Source under the terms of this LIcense in one of these ways a Convey the object code in or embodied in a physical product including a physical distribution medium accompanied by the Corresponding Source fixed on a durable physical medium customarily used for software interchange b Convey the object code in or embodied in a physical product including a physical distribution medium accompanied by a written offer valid for at least three years and valid for as long as you offer spare parts or customer support for that product model to give anyone who possesses the object code either 1 a copy of the Corresponding Source for all the software in the
9. Page replaces the Home Page Menu Button Figure 2 4 Menu Button The Menu Button presents a list of menu items allowing you to choose a particular function Options References About Zonal Operation Continuous Flow Operation e Service Mode The menu options are explained in the following chapter PN B04155AC The Touch Screen Interface 2 Areas on the Screen Status Display Figure 2 5 Ready Status The Status Display is in the middle of the Header Bar and shows you the current instrument status The background color of the header bar changes with the type of status e Blue background system ready no run in progress Figure 2 6 Header Bar Ready Status e Green background in operation run in progress Figure 2 7 Header Bar Running Status e Green background in operation stop in progress Figure 2 8 Header Bar Stopping Status Stopping e Yellow background warning message Figure 2 9 Header Bar Warning Message e Red background fatal error message Figure 2 10 Header Bar Fatal Error Message Diagnostic Pending PN B04155AC 2 3 The Touch Screen Interface Areas on the Screen Help Button Figure 2 11 Help Button The Help button gives you access to the built in online help messages Help messages are described at the end of this chapter Footer Bar The bottom of the touch screen always shows the footer bar The footer bar appears in two differ
10. Set Speed 50 000 RPM Set Time Hold Set Temp 25 C B The Select Rotor and Labware Page appears when you select the Button on either the Set Speed Page or the Set Speed Time Page allowing you to select a rotor for the next run This is different from the Rotor Catalog Page which shows rotor specifications but does not select one for your next run In addition to the Header Bar and the Footer Bar the Select Rotor and Labware Page contains the following elements The Rotor Catalog List shows the available rotors for the instrument You can select a rotor from this list If the No Rotor value is selected you cannot use RCF units on the Set Speed Page e The Cancel Button dismisses the page and any changes e The OK Button accepts the selection and dismisses the page For details about each rotor or labware use the Rotor Catalog Page This page only lists names for selection PN B04155AC 4 11 Functional Pages References Page References Page 4 12 Figure 4 14 References Page Set Time 0 05 h mm The References Page appears when you select the References Button in the Menu Page allowing you to choose from the available reference materials to view In addition to the Header Bar and the Footer Bar the References Page contains the following elements The Rotor Catalog button takes you to the Rotor Catalog Page The Labware Catalog button takes you to the Labware Catalog Page The Export User Manual
11. Temperature of the run in degrees Celsius The Vacuum Display Button on the Footer Bar shows the current vacuum of the run that is in progress The button toggles between evacuating and venting Press it before starting a run after the door is closed to begin evacuating and pre conditioning the chamber Press it at the end of a run assuming speed is less than 3000 rpm and decelerating to release the vacuum i e vent the chamber Press the Accel acceleration button on the Footer Bar to select a new profile for the acceleration of arun Press the Decel deceleration button on the Footer Bar to select a new profile for the deceleration of arun 4 29 Functional Pages Running Home Page 4 30 PN BO4155AC CHAPTER 5 Maintenance and Troubleshooting E This chapter contains care and maintenance procedures to be performed regularly Field Service For any maintenance not covered in this manual contact Beckman Coulter Field Service for assistance USA customers can call 1 800 742 2345 For international contacts see the website at www beckmancoulter com or use the contact numbers on the inside front cover of this book NOTE Itis your responsibility to decontaminate the instrument as well as any rotors and accessories before requesting service by Beckman Coulter Field Service Rotors and Labware You also need to maintain rotors and labware You can find the approved rotors and labware on the Reference Page as described
12. USB drive while a data transfer is in progress e The Available Drives list shows all the available USB drives If the list is empty the prompt Insert USB Drive appears below the list area Select an available USB drive to receive the information The Export Button transfers the information to the selected USB drive 4 25 4 Functional Pages Compatible Tubes for Rotor Page Compatible Tubes for Rotor Page Figure 4 28 Compatible Tubes for Rotor Page gt f Ready Compatible Tubes for Rotor Type 100 Ti Volume Max Speed 100 000 RPM K Factor 15 1 5 6 mL Maximum RCF 801 900 xg be Sd RCF Ravg 622 200 xg Quick Seal RCF Rmin 442 400 xg Quick Seal Tube Capacity 5 6 mL Total Capacity 44 8 mL Tube Count 6 Set Speed 15 000 RPM Set Time 0 05 h mm Set Temp 25 C The Compatible Tubes for Rotor Page appears when you select the Labware Button on the Rotor Catalog Page allowing you to examine the detailed specifications for the labware for the selected rotor This is different from the Labware Catalog Page which shows the labware for all rotors In addition to the Header Bar and the Footer Bar the Compatible Tubes for Rotor Page contains the following elements e The List of Labware on the left side of the screen shows the labware for the selected rotor by volume type and part number Scroll through this list and select a particular labware to view the detailed information about it
13. device Refer to the End User License Agreement for Microsoft Windows XP Server for additional information The End User License Agreement is available on the Microsoft Embedded Systems website Biohazard If a hazardous substance such as blood is spilled onto the instrument clean up the spill by using a 10 bleach solution or use your laboratory decontamination solution Then follow your laboratory procedure for disposal of hazardous materials If the instrument needs to be decontaminated contact your Beckman Coulter Representative Risk of chemical injury from bleach To avoid contact with the bleach use barrier protection including protective eyewear gloves and suitable laboratory attire Refer to the Safety Data Sheet for details about chemical exposure before using the chemical NOTE For Safety Data Sheets SDS MSDS information go to the Beckman Coulter website at www beckmancoulter com X PN B04155AC Safety and Notices ROHS Notice ROHS Notice These labels and materials declaration table the Table of Hazardous Substance s Name and Concentration are to meet People s Republic of China Electronic Industry Standard SJ T11364 2006 Marking for Control of Pollution Caused by Electronic Information Products requirements China RoHS Caution Label ost E5 AAS0S6 AD ik AM Mig Date This label indicates that the electronic information product contains certain toxic or hazardous substances The center numb
14. in the previous chapter Refer to the applicable rotor and labware documents for detailed instructions on their care Cleaning The methods and materials used in the following procedures have been tested by Beckman Coulter and will not damage the instrument if used as instructed Before using any other materials or methods check with Beckman Coulter to verify that they will not damage the instrument PN B04155AC 5 1 Maintenance and Troubleshooting Decontamination Instrument Surfaces Clean instrument surfaces using a cloth dampened with a mild detergent solution such as Beckman Solution 555 Be careful not to spill liquid on the instrument where electrical or mechanical components could get damaged Rotor Chamber The rotor chamber is coated with epoxy resin paint To clean the chamber wipe it with a cloth dampened with a mild detergent such as Beckman Solution 555 Chamber Door O ring The chamber door O ring is Buna N rubber Clean it with a tissue or soft cloth every 3 or 4 months If the O ring becomes worn or damaged replace it Lightly coat the new O ring with silicone vacuum grease 335148 to ensure an optimum vacuum seal NOTE Instrument O rings have not been designed as bioseals for aerosol containment Decontamination If the instrument and or accessories are contaminated with radioactive or pathogenic solutions follow appropriate decontamination procedures as determined by your laboratory safety officer Re
15. in vitro diagnostic testing as well as nonhuman body samples and chemicals including industrial and environmental samples This centrifuge should be operated by qualified personnel only Touch Screen The touch screen is both the information display and the control input for the instrument As they are needed control buttons appear on the screen When you select a button you activate that control The touch screen position is adjustable both swivel and tilt to be made viewable from nearly anywhere in the lab This is especially useful in environments where a single operator may oversee a number of instruments Each component of the touch screen interface is explained in the following chapters Name Rating Plate A name rating plate is affixed to the rear of the instrument Always mention the serial number and model number available on the About Page when contacting Beckman Coulter regarding your instrument PN B04155AC 1 1 Description Rotor Chamber Rotor Chamber The rotor chamber is made of aluminum and coated with a chemical resistant epoxy finish The central feature is the rotor drive spindle but several sensors and control systems are also contained in the rotor chamber Vacuum System The Optima XE uses a diffusion pump in series with a mechanical vacuum pump to reduce chamber pressure to a very low level The system starts automatically when you start a run or manually when the chamber door is closed and you us
16. license GNU GENERAL PUBLIC LICENSE Version 3 29 June 2007 Copyright 2007 Free Software Foundation Inc lt http fsf org gt Everyone is permitted to copy and distribute verbatim copies of this license document but changing it is not allowed Preamble The GNU General Public License is a free copyleft license for software and other kinds of works The licenses for most software and other practical works are designed to take away your freedom to share and change the works By contrast the GNU General Public License is intended to guarantee your freedom to share and change all versions of a program to make sure it remains free software for all its users We the Free Software Foundation use the GNU General Public License for most of PN B04155AC Third Party Contributions and Legal Notices D our software it applies also to any other work released this way by its authors You can apply it to your programs too When we speak of free software we are referring to freedom not price Our General Public Licenses are designed to make sure that you have the freedom to distribute copies of free software and charge for them if you wish that you receive source code or can get it if you want it that you can change the software or use pieces of it in new free programs and that you know you can do these things To protect your rights we need to prevent others from denying you these rights or asking you to surrender the rights Theref
17. specified reasonable legal notices or author attribution in that material or in the Appropriate Legal Notices displayed by works containing it or c Prohibiting misrepresentation of the origin of that material or requiring that modified versions of such material be marked in reasonable ways as different from the original version or d Limiting the use for publicity purposes of names of licensors or authors of the material or e Declining to grant rights under trademark law for use of some trade names trademarks or service marks or f Requiring indemnification of licensors and authors of that material by anyone who conveys the material or modified versions of it with contractual assumptions of liability to the recipient for any liability that these contractual assumptions directly impose on those licensors and authors All other non permissive additional terms are considered further restrictions within the meaning of section 10 If the Program as you received it or any part of it contains a notice stating that it is governed by the License along with a term that is a further restriction you may remove that term If a license document contain s a further restriction but permits relicensing or conveying under this License you may add to a covered work material governed by the terms of that license document provided that the further restriction does not survive such relicensing or conveying If you add terms to a co
18. supply too low lt 5 5VDC Call Beckman Coulter Field Service D210 Power Supply SCB 12V supply too high gt 13 2VDC or SCB 12V supply too low lt 10 8VDC Call Beckman Coulter Field Service D211 Power Supply SCB 3 3V supply too high gt 3 63VDC Call Beckman Coulter Field Service C 3 C Diagnostics Diagnostics User Messages Chart Table C 1 Diagnostics User Messages Chart Continued Message Definition Result Recommended Action D212 Power Supply SCB analog 3 3V supply too high gt 3 63VDC or SCB analog 3 3V supply too low lt 2 97VDC Call Beckman Coulter Field Service D213 Power Supply System 24V supply too high gt 26 4VDC or System 24V supply too low lt 21 6VDC Call Beckman Coulter Field Service D214 Power Supply System 5V supply too high gt 5 5VDC Call Beckman Coulter Field Service D216 AC Power Out of Range AC Power Out of Range Check electrical power source A217 Alert AC Power Voltage Sag AC voltage sagged below 180Vac or AC voltage frequency is out of range during braking None D300 Rotor Speed Rotor speed exceeds maximum rated speed Call Beckman Coulter Field Service A301 Alert Rotor Speed Adjusted Rotor set speed adjusted None A302 Alert Inertia Calibration Inertia calibration failure Call Beckman Coulter Field Service D303 S
19. the envelope clear of any persons or objects and do not reach into the envelope except when required to change operating controls vi PN B04155AC Safety and Notices Safety Notices Replacement Parts Do not replace any centrifuge components with parts not specified for use on this instrument Service Any servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury Make sure that the power switch is turned off and the instrument is disconnected from the main power source by removing its power plug from the receptacle Refer such servicing to qualified personnel NOTE It is your responsibility to decontaminate the instrument and accessories before requesting service by Beckman Coulter Field Service Power Supply To reduce the risk of electrical shock this instrument uses a three wire electrical cord and plug to connect this equipment to earth ground Make sure that the matching wall outlet receptacle is properly wired and earth grounded Fuse Replacement Fuses protect certain electrical circuits within this instrument against overcurrent conditions The fuse is not customer replaceable For continued protections please contact Beckman Coulter Field Service PN B04155AC vii Safety and Notices Safety Notices Mechanical Safety For safe operation of the equipment observe the following Use only the Beckman Coulter rotors and
20. the following elements e The Set Speed Display at the top of the page shows the current Set Speed in RPM or RCF The last two digits are always zeroes when the speed is above 1000 RPM e The Keypad changes the speed setting You can use the Clear and Backspace keys to make corrections 4 5 Functional Pages Set Time Page The RPM RCF Button selects the type of speed units This button is disabled until a rotor is selected The Select Rotor Button takes you to the Select Rotor and Labware Page The Cancel Button discards your changes and dismisses the page The OK Button accepts your changes and dismisses the page Set Time Page 4 6 Figure 4 9 Set Time Page Set Time Set Speed 15 000 RPM Set Time Hold The Set Time Page appears when you select the Set Time Display Button on the Home Page or the Footer Bar allowing you to set the duration for the next run If the instrument is in t mode the Set Speed Time Page appears instead In addition to the Header Bar and the Footer Bar the Set Time Page contains the following elements The Set Time Display shows the current Set Time setting in hours and minutes The Keypad changes the time setting You can use the Clear and Backspace keys to make corrections The Cancel Button discards your changes and dismisses the page without changing the current values The OK Button accepts your changes and dismisses the page PN B04155AC Functional Pages 4 Set S
21. the work are being offered to the general public at no charge under subsection 6d A separable portion of the object code whose source code is excluded from the Corresponding Source as a System Library need not be included in conveying the object code work PN B04155AC Third Party Contributions and Legal Notices D A User Product is either 1 a consumer product which means any tangible personal property which is normally used for personal family or household purposes or 2 anything designed or sold for incorporation into a dwelling In determining whether a product is a consumer product doubtful cases shall be resolved in favor of coverage For a particular product received by a particular user normally used refers to a typical or common use of that class of product regardless of the status of the particular user or of the way in which the particular user actually uses or expects or is expected to use the product A product is a consumer product regardless of whether the product has substantial commercial industrial or non consumer uses unless such uses represent the only significant mode of use of the product Installation Information for a User Product means any methods procedures authorization keys or other information required to install and execute modified versions of a covered work in that User Product from a modified version of its Corresponding Source The information must suffice to ensure that th
22. you to the Select Rotor and Labware Page e The Keypad changes slightly depending on the value you are entering For setting time use the Hold key to set a hold value For setting w t use the decimal and e keys to enter values in exponential notation The Cancel Button discards your changes and dismisses the page e The OK Button accepts your changes and dismisses the page PN B04155AC 4 7 Functional Pages Set Temperature Page Set Temperature Page Set Temperature 25 Set Speed 15 000 RPM Set Time 0 05 h mm Set Temp 25 C The Set Temperature Page appears when you select the Set Temperature Button on the Home Page or the Footer Bar allowing you to set the temperature for the next run In addition to the Header Bar and the Footer Bar the Set Temperature Page contains the following elements e The Set Temperature Display shows the current Set Temperature setting in degrees Celsius e The Keypad changes the temperature setting e The Cancel Button discards your changes and dismisses the page e The OK Button accepts your changes and dismisses the page To precondition the chamber to the selected temperature use the Vacuum Button Display on the Footer Bar of the Home Page 4 8 PN B04155AC Functional Pages 4 Set Acceleration Deceleration Profiles Page Set Acceleration Deceleration Profiles Page Figure 4 12 Set Acceleration Deceleration Profiles Page Maximum Deceleration Set Speed 15 0
23. 00 RPM Set Time 0 05 hmm The Set Acceleration Deceleration Profiles Page appears when you select the Accel Decel Button Display in the Footer Bar allowing you to choose profiles for acceleration and deceleration In addition to the Header Bar and the Footer Bar the Set Acceleration Deceleration Profiles Page contains the following elements e Acceleration lets you to choose an acceleration value by selecting a number Deceleration lets you choose a deceleration value by selecting a number e The Cancel Button discards your changes and dismisses the page e The OK Button accepts your changes and dismisses the page The acceleration values are the degree of reduction from the maximum value The acceleration value of zero is the maximum no reduction The acceleration value of 9 is the slowest maximum reduction The same applies to the deceleration values The value 10 is an absolute reduction eliminating all braking and allowing the rotor to coast to a stop Slower numerically higher acceleration and deceleration values minimize sample to gradient interface disturbance Each acceleration profile has a designated time that it takes to reach a specified speed After that it uses maximum acceleration to reach run speed For a deceleration profile the instrument uses maximum deceleration until it reaches the specified speed then takes the designated time to slow to a stop PN B04155AC 4 9 Functional Pages Set Acceleration Decelera
24. CE PN B04155AC January 2015 Beckman Coulter Inc aal 250 S Kraemer Blvd Brea CA 92821 U S A User Guide Optima XE Series Preparative Ultracentrifuges For In Vitro Diagnostic Use Rx Only B COULTER Optima XE Ultra Centrifuge User Guide PN B04155AC January 2015 2015 Beckman Coulter Inc All rights reserved Trademarks Beckman Coulter the stylized logo and Optima are trademarks of Beckman Coulter Inc and are registered with the USPTO All other trademarks service marks products or services are trademarks or registered trademarks of their respective holders Find us on the World Wide Web at www beckmancoulter com EC REP Beckman Coulter Ireland Inc Mervue Business Park Mervue Galway Ireland 353 91 774068 Printed in U S A Revision History B04155AA Initial Issue 05 2011 Issue Version AB 01 2014 Manual updated per Canadian Standards Association CSA guidelines Issue Version AC 01 2015 Changes or additions were made to Getting Access to the Sample Restoring the Instrument to Operating Condition Note Changes that are part of the most recent revision are indicated in text by a bar in the margin of the amended page This document applies to the latest software listed and higher versions When a subsequent software version affects the information in this document a new issue will be released to the Beckman Coulter website For labeling updates go to www beckm
25. HE EXTENT PERMITTED BY APPLICABLE LAW EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT HOLDERS AND OR OTHER PARTIES PROVIDE THE PROGRAM AS IS WITHOUT WARRANTY OF ANY KIND EITHER EXPRESSED OR IMPLIED INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM IS WITH YOU SHOULD THE PROGRAM PROVE DEFECTIVE YOU ASSUME THE COST OF ALL NECESSARY SERVICING REPAIR OR CORRECTION 16 Limitation of Liability IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING WILL ANY COPYRIGHT HOLDER OR ANY OTHER PARTY WHO MODIFIES AND OR CONVEYS THE PROGRAM AS PERMITTED ABOVE BE LIABLE TO YOU FOR DAMAGES INCLUDING ANY GENERAL SPECIAL INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE USE OR INABILITY TO USE THE PROGRAM INCLUDING BUT NOT LIMITED TO LOSS OF DATA OR DATA BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES 17 Interpretation of Sections 15 and 16 If the disclaimer of warranty and limitation of liability provided above cannot be given local legal effect according to their terms reviewing courts shall apply local law that most closely approximates an absolute waiver of all civil liability in connection with the Program unless a warra
26. Hz e Max Output Current 85A e Max Fault Current 20A PN B04155AC PN B04155AC Description Specifications Specification Description Environmental e RoHS compliant material 100 of content e Cooling system Thermoelectric no CFC gases used e Noise output 1m in front of instrument lt 51 dBA Sound pressure level 7 0 sones Zwicker Loudness e Maximum heat dissipation into room under steady state conditions 3400 Btu hr 1 0 kW e Pollution degree 2a e HEPA filter Available a Normally only non conductive pollution occurs occasionally however a temporary conductivity caused by condensation must be expected 1 7 1 Description Available Rotors Audible Sounds The Optima XE instrument will make an audible sound while displaying a corresponding visual prompt with the following events e Boot up e Start of Run e End of Run Diagnostics Alert e Vacuum low enough to open door Door open during Zonal or Continuous Flow mode Available Rotors All currently manufactured Beckman Coulter floor model ultracentrifuge rotors can be used in the Optima XE ultracentrifuge The rotors are described in individual manuals that accompany each rotor Information on rotors and accessories is available in the Rotor Catalog accessible from the touch screen Additional information is available in Rotors and Tubes for Preparative Ultracentrifuges
27. LR IM and in the Beckman Coulter Ultracentrifuge Rotors Tubes amp Accessories catalog publication BR 8101 The Beckman Coulter website http www beckmancoulter com contains additional information about centrifugation processes and products Refer to specific rotor manuals for proper installation and removal of rotors from the instrument 1 8 PN B04155AC CHAPTER 2 The Touch Screen Interface _ This chapter describes the touch screen interface for the Optima XE Except for the power switch all the controls for the instrument are programmed controls that appear on the touch screen Figure 2 1 Home Page Set Speed 100 000 RPM Set Time 0 01 hmm Set Temp 25 C Areas on the Screen The items on the screen may change depending on the state of the instrument There are three areas where items appear on the screen e The Header Bar e The Footer Bar e The Page Display Area PN B04155AC 2 1 The Touch Screen Interface Areas on the Screen Header Bar 2 2 Figure 2 2 Header Bar The area across the top of the screen is called the Header Bar containing four important items e Home Page Button e Menu Button e Status Display e Help Button The following sections explain these items Home Page Button Figure 2 3 Home Page Button a The Home Page Button takes you to the Home Page from any other page When you use Zonal Mode or Continuous Flow Mode the Zonal Continuous Flow Operation
28. N Release the Door Lock Locate the door interlock assembly Pull down on the interlock pin until it locks in the downward position Open the Door Open the chamber door carefully If the rotor is still spinning even at a slow speed close the door and wait PN B04155AC Maintenance and Troubleshooting 5 Retrieving Your Sample in Case of Power Failure NEVER attempt to slow or stop the rotor by hand With the door open and the rotor stopped you can retrieve your sample Do not attempt to run the instrument before restoring it to a safe operating condition as described in the following section Restoring the Instrument to Operating Condition After retrieving your sample restore the instrument to operating condition as follows Close the chamber door N Close the vent e For Configuration A with vent port cap Replace and tighten the vacuum vent port cap It should be snug but do not overtighten it For Configuration B with solenoid release screw Turn the screw counter clockwise but do not overtighten it 3 Place the front panel in the tilted position with the top edge toward you the sides aligned with the sides of the instrument and the tabs at the bottom edge inserted into the lip at the base of the instrument 4 Lift the front edge of the top panel a few inches and insert the upper edge of the front panel under it Push back gently to engage the tabs then push down 5 For each of
29. Retrieving Your Sample in Case of Power Failure When working on the inside of the instrument be careful not to touch any wires or circuitry 5 Determine the instrument s vacuum configuration There are two possible configurations Configuration A will vent the vacuum through a vent port cap refer to Figure 5 2 Configuration B will vent the vacuum throught a solenoid release screw refer to Figure 5 3 Figure 5 2 Internal Parts Configuration A vents through the port cap 1 Fan Housing 2 Vacuum Vent Port Cap 3 Door Interlock Assembly 5 6 PN B04155AC Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure Figure 5 3 Internal Parts Configuration B vents through the vacuum solenoid release screw 1 Fan Housing 2 Vacuum solenoid release screw 3 Door Interlock Assembly Also refer to To Vent the Chamber Configuration A with vent port cap and To Vent the Chamber Configuration B with solenoid release screw 6 Listen carefully for any sounds coming from the drive and touch the fan housing to feel for vibrations If you detect any sounds or vibrations do not go further The rotor is still spinning and you must wait for it to come to a stop PN B04155AC 5 7 5 Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure 5 8 Vent the Chamber Although you checked for noise and vibration there is still a p
30. a higher group require more than one level of protection Dispose of all waste solutions according to appropriate environmental health and safety guidelines Do not centrifuge flammable or explosive vapors or materials capable of hazardous chemical reactions PN B04155AC Safety and Notices Certification Precautions with Liquids Do not place containers holding liquid on or near the chamber door Liquid if spilled may get into the instrument and damage electrical or mechanical components Volatile Liquids This instrument is not designed for use with materials capable of developing flammable or explosive vapors or hazardous chemical reactions Do not centrifuge such materials for example chloroform or ethyl alcohol in this instrument nor handle or store them near the centrifuge Certification To ensure full system quality Beckman Coulter Optima XEs have been manufactured in a registered ISO 9001 or 13485 facility They have been designed and tested to conform to when used with Beckman Coulter rotors the laboratory equipment requirements of applicable regulatory agencies Declarations of conformity and certificates of compliance are available at www beckmancoulter com Scope of Manual This manual is designed to familiarize you with the Optima XE its functions specifications operation and routine operator care and maintenance Read this entire manual especially the safety notices and all safety related information
31. accessories designed for use in this instrument Do not exceed the maximum rated speed of the rotor in use NEVER attempt to slow or stop a rotor by hand Do not move the centrifuge while the drive motor is spinning In the event of a power failure do not attempt to retrieve the sample from the instrument for at least one hour Then follow the instructions for recovery of the sample in the Maintenance and Troubleshooting chapter Chemical and Biological Safety Normal operation may involve the use of solutions and test samples that are pathogenic toxic or radioactive Such materials require that you take all necessary safety precautions viii Handle body fluids with care because they can transmit disease No known test offers complete assurance that they are free of micro organisms Handle all infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Some of the most virulent infectious agents Hepatitis B and C and HIV I V viruses atypical mycobacteria and certain systemic fungi require additional emphasis on aerosol protection Do not run toxic pathogenic or radioactive materials in a rotor without taking appropriate safety precautions Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual require biosafe containment Materials of
32. alert you to this condition 1 Description Specifications Specifications Only values with tolerances or limits are guaranteed data Values without tolerances are informative data without guarantee Control Features Specifications Description Speed e Set speed 1 000 to rated speed in 100 rpm increments e Maximum speed XE 90 90 000 rom XE 100 100 000 rom Speed display Actual measured rotor speed in 10 rom increments below 1000 rpm and 100 rpm increments 1000 rpm Speed control Actual measured rotor speed 2 rom at steady state 1 000 rpm to rated speed e Acceleration 10 profiles e Deceleration 11 profiles including coast Braking Regenerative power reclamation Time e Set time Upto 999 hours 59 minutes including Hold runs Upto 3 94780 x 10 14 radians squared per second in wt mode e Time display Indicates time remaining in timed runs time elapsed in Hold runs or estimated time remaining in 7t runs e Time accuracy 70 ppm 6 seconds day e Run modes Time t and RCF 1 4 PN BO4155AC Description Specifications Specifications Description Temperature Set temperature 0 to 40 C in 1 C increments e Rotor Temperature after equilibration 0 5 C over entire set temperature range for all rotors except SW 32 0 8 C over entire set temperature range for SW 32 Temperature display
33. ancoulter com and download the latest version of the manual or system help for your instrument PN B04155AC Revision History PN B04155AC Safety and Notices This chapter presents the important notices that apply to the instrument and describes the conventions used in the document Alerts for Danger Warning Caution Important and Note DANGER indicates an imminently hazardous situation which if not avoided will result in death or serious injury This signal word is to be limited to the most extreme situations WARNING indicates a potentially hazardous situation which if not avoided could result in death or serious injury May be used to indicate the possibility of erroneous data that could result in an incorrect diagnosis does not apply to all products CAUTION indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury It may also be used to alert against unsafe practices May be used to indicate the possibility of erroneous data that could result in an incorrect diagnosis does not apply to all products IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed Following the advice in the Important adds benefit to the performance of a piece of equipment or to a process NOTE NOTE is used to call attention to notable information that should be followed during installation use or servicing of this equipment PN B04155AC V Safety a
34. ark License This license does not grant you rights to use any contributors name logo or trademarks b Ifyou bring a patent claim against any contributor over patents that you claim are infringed by the software your patent license from such contributor to the software ends automatically c If you distribute any portion of the software you must retain all copyright patent trademark and attribution notices that are present in the software d Ifyou distribute any portion of the software in source code form you may do so only under this license by including a complete copy of this license with your distribution If you distribute any portion of the software in compiled or object code form you may only do so under a license that complies with this license e The software is licensed as is You bear the risk of using it The contributors give no express warranties guarantees or conditions You may have additional consumer rights under your local laws which this license cannot change To the extent permitted under your local laws the contributors exclude the implied warranties of merchantability fitness for a particular purpose and non infringement D VNC Free Edition 4 1 3 Copyright 2002 2008 RealVNC Limited is included in its unmodified binary form as aggregate work A copy of this work in source form may be obtained at no cost by contacting Beckman Coulter Technical Support This work is made available under the following
35. ations that contain the covered work unless you entered into that arrangement or that patent license was granted prior to 28 March 2007 Nothing in this license shall be construed as excluding or limiting any implied license or other defenses to infringement that may otherwise be available to you under applicable patent law 12 No Surrender of Others Freedom If conditions are imposed on you whether by court order agreement or otherwise that contradict the conditions of this License they do not excuse you from the conditions of this License If you cannot convey a covered work so as to satisfy simultaneously your obligations under this License and any other pertinent obligations then as a consequence you may not convey it at all For example if you agree to terms that obligate you to collect a royalty for further conveying from those to whom you convey the Program the only way you could satisfy both those terms and this License would be to refrain entirely from conveying the Program 13 Use with the GNU Affero General Public License Notwithstanding any other provision of this License you have permission to link or combine any covered work with a work license under version 3 of the GNU Affero General Public License into a single combined work and to convey the resulting work The terms of this License will continue to apply to the part which is the covered work but the special requirements of the GNU Affero General Public License sec
36. before operating the instrument or performing maintenance NOTE If the instrument is used in a manner other than specified in this manual the safety and performance of this equipment could be impaired Further the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety Use of any equipment not specifically recommended in this manual and or the appropriate rotor manual is the sole responsibility of the user PN B04155AC Safety and Notices CFC Free Centrifugation CFC Free Centrifugation To ensure minimal environmental impact no CFCs are used in the manufacture or operation of the Optima XE ultracentrifuge Software Copyright The software and other information incorporated into the Optima XE is protected by international copyright laws Unauthorized copying use distribution transfer or sale is a violation of those laws that may result in civil or criminal penalties This computer program is also subject to additional restrictions contained in the following Microsoft OEM Customer License Agreement for Embedded Systems If you use the Device to access or utilize the services or functionality of Microsoft Windows XP Server all editions or use the Device to permit workstation or computing devices to access or utilize the services or functionality of Microsoft Windows XP Server you may be required to obtain a Client Access License for the Device and or each such workstation or computing
37. button takes you to the Export User Manual Page The Done Button dismisses the page PN B04155AC Functional Pages System Options Page System Options Page Set Speed 15 000 RPM Set Time 0 05 hmm Set Temp 25 C The System Options Page appears when you select the Options Button in the Menu Page allowing you to choose any of the available system option setting pages In addition to the Header Bar and the Footer Bar the System Options Page contains the following elements PN B04155AC The Select Language Button takes you to the Select Language Page The Set Sound Button takes you to the Set Sound Page The Diagnostic History Button takes you to the Diagnostic History Page The Set Date and Time button takes you to the Set Date and Time Page The Set System Name Button takes you to the Set System Name Page The t Mode Button toggles the m t mode off and on The mode is on when the button is highlighted See the Set Speed Time Page for details The Done Button dismisses the page 4 13 4 Functional Pages Zonal Authorization Page Zonal Authorization Page Figure 4 16 Zonal Authorization Page Zonal Authorization Authorize eeese Set Time Hold Set Temp 25 C The Zonal Authorization Page appears when you select the Zonal Operation Button on the Menu Page allowing you to use Zonal mode for the next run In addition to the Header Bar and the Footer Bar the Zonal Authorization Page contains the foll
38. d reserved by Beckman Coulter Inc A AdornedControl available at lt http www codeproject com KB WPF adornedcontrol aspx gt by Ashley Davis the standard version included in its binary form linked with other modules This work is made available under the Code Project Open License 1 02 CPOL available at lt http www codeproject com info cpol10 aspx gt By exercising any rights to AdornedControl the recipient accepts and agrees to be bound by the terms of CPOL B PasswordAssistant available at lt http blog functionalfun net 2008 06 wpf passwordbox and data binding html gt and ListBoxSelectedItemsBinding available at lt http blog functionalfun net 2009 02 how to databind to selecteditems html gt both by Samuel Jack These works are made available under the Creative Commons Attribution 2 0 England and Wales License available at lt http creativecommons org licenses by 2 0 uk gt C BalancedWrapPanel Copyright Microsoft Corporation available at lt http blogs msdn com b delay archive 2009 12 13 wrap music a more flexible balanced wrappanel implementation for silverlight and wpf aspx gt by David Anson MvvmFoundation WPF available at lt http mvvmfoundation codeplex com SourceControl list changesets gt by Josh Smith and modified portions of MvvnFoundation WPF contained in MvvmFoundatiopn MH Changes zip available at lt http mvvmfoundation codeplex com workitem 5453 gt by Matt Heffron These works a
39. dge that but for the patent license your conveying the covered work in a country or your recipient s use of the covered work in a country would infringe one or more identifiable patents in that country that you have reason to believe are valid If pursuant to or in connection with a single transaction arrangement you convey or propagate by procuring conveyance of a covered work and grant a patent license to some of the parties receiving the covered work authorizing them to use propagate modify or convey a specific copy of the covered work then the patent license you grant is automatically extended to all recipients of the covered work and works based on it A patent license is discriminatory if it does not include within the scope of its coverage prohibits the exercise of or is conditioned on the non exercise of one or more of the rights that are specifically granted under this License You may not convey a covered work if you are a party to an arrangement with a third party that is in the business of distributing software under which you make payment to the third party based on the extent of your activity of conveying the work and under which the third party grants to any of the parties who would receive the covered work from you a discriminatory patent license a in connection with copies of the covered work conveyed by you or copies made from those copies or b primarily for and in connection with specific products or compil
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41. e current run speed setting in small characters across the bottom The Set Speed Display Button takes you to the Set Speed Page or to the Set Speed Time Page w t Mode if the wt mode is active When you are not on the Home page the Set Speed Display Button appears in the Footer Bar Set Time Display Button Figure 4 4 Set Time Display Button Set Time 0 05 h mm The Set Time Display Button shows the current remaining run time as a large number and also shows the current run duration setting in small characters across the bottom Before you begin a run the numbers are the same During a run the large number counts down to zero When you select the Set Time Display Button it takes you to the Set Time Page Set Speed Time Page wt Mode if the wt mode is active When you are not on the Home page the Set Time Display Button appears in the Footer Bar Set Temp Display Button Figure 4 5 Set Temp Display Button Set Temp 25 C The Set Temp Display Button shows the current rotor temperature as a large number and also shows the current temperature setting in small characters across the bottom When you select the Set Temp Display Button it takes you to the Set Temperature Page When you are not on the Home page the Set Temp Display Button appears in the Footer Bar PN BO4155AC 4 3 Functional Pages Menu Page Menu Page Figure 4 6 Menu Page The Menu Page appears when you select the Menu Butt
42. e arrow button above one of the numbers to make it larger or select the button below to make it smaller The Hour Minute AM PM controls let you set the time They work the same as the date controls except that the AM PM value has only one arrow button to change the setting The Select Time Format Buttons set the way time appears on the instrument The 12 Hour format button selects a 12 hour display with AM and PM The 24 Hour format button selects a 24 hour display without AM and PM notation With some language selections the 12 hour option may be unavailable The Cancel Button discards any changes you made on this screen and returns to the System Options Page The OK Button accepts your changes and returns to the System Options Page PN B04155AC Functional Pages 4 Set Sound Page Set Sound Page Figure 4 25 Set Sound Page Set Sound Volume Key Click Mute Low Medium High Set Speed 15 000 RPM Set Time 0 05 himm The Set Sound Page appears when you select the Set Sound Button on the System Options Page allowing you to set the sound volume and enable or disable the key click option In addition to the Header Bar and the Footer Bar the Set Sound Page contains the following elements e The Volume buttons let you to choose one of the four system volumes Mute silent Low Medium or High The Key Click buttons let you choose if the key click is On or Off The Cancel Button discards your changes and return
43. e continued functioning of the modified object code is in no case prevented or interfered with solely because modification has been made If you convey an object code work under this section in or with or specifically for use in a User Product and the conveying occurs as part of a transaction in which the right of possession and use of the User Product is transferred to the recipient in perpetuity or for a fixed term regardless of how the transaction is characterized the Corresponding Source conveyed under the section must be accompanied by the Installation Information But this requirement does not apply if neither you nor any third party retains the ability to install modified object code on the User Product for example the work has been installed in ROM The requirement to provide Installation Information does not include a requirement to continue to provide support service warranty or updates for a work that has been modified or installed by the recipient or for the User Product in which it has been modified or installed Access to a network may be denied when the modification itself materially and adversely affects the operation of the network or violates the rules and protocols for communication across the network Corresponding Source conveyed and Installation Information provided in accord with this section must be in a format that is publicly documented and with the implementation available to the public in source code form and m
44. e substantially in other domains we stand ready to extend this provision to those domains in future versions of the GPL as needed to protect the freedom of users Finally every program is threatened constantly by software patents States should not allow patents to restrict development and use of software on general purpose computers but in those that do we wish to avoid the special danger that patents applied to a free program could make it effectively proprietary To prevent this the GPL assures that patents cannot be used to render the program non free The precise terms and conditions for copying distribution and modification follow TERMS AND CONDITIONS 0 Definitions This License refers to version 3 of the GNU General Public License Copyright also means copyright like laws that apply to other kinds of works such as semiconductor masks The Program refers to any copyrightable work licensed under this License Each licensee is addressed as you Licensees and recipients may be individuals or organizations PN B04155AC D 3 Third Party Contributions and Legal Notices D 4 To modify a work means to copy from or adapt all or part of the work in a fashion requiring copyright permission other than the making of an exact copy The resulting work is called a modified version of the earlier work or a work based on the earlier work A covered work means either the unmodifi
45. e the Vacuum Display Button on the Footer Bar of the Home Page When the vacuum system is on the chamber pressure is displayed in microns in the Vacuum Display Button At the end of a run press the Vacuum Display Button to vent the chamber vacuum before attempting to open the door When the chamber vacuum is reduced to the point that you can open the door the system sounds an audible tone After you open the door there is a five minute period where the chamber temperature remains as set to allow back to back runs Then the chamber returns to approximate room temperature to minimize condensation collecting in the chamber To help keep the chamber dry and clean keep the door closed whenever possible If you wish to vent the chamber before the rotor has come to a complete stop you can do so as soon as it slows below 3000 rpm In zonal or continuous flow operation the door may remain open while the rotor is spinning up to the loading speed The vacuum system is activated when the door is closed and the rotor speed is above 3000 rpm after loading the rotor Normally you vent the chamber at the end of the run when the rotor has decelerated to the unloading speed but you can vent the chamber when the rotor has decelerated below 3000 rpm if the unloading speed is slower than that Temperature Sensing and Control A solid state thermopile in the bottom of the rotor chamber monitors rotor temperature The Optima XE uses a solid state thermoelectr
46. ed Program or a work based on the Program To propagate a work means to do anything with it that without permission would make you directly or secondarily liable for infringement under applicable copyright law except executing it on a computer or modifying a private copy Propagation includes copying distribution with or without modification making available to the public and in some countries other activities as well To convey a work means any kind of propagation that enables other parties to make or receive copies Mere interaction with a user through a computer network with no transfer of a copy is not conveying An interactive user interface displays Appropriate Legal Notices to the extent that it includes a convenient and prominently visible feature that 1 displays an appropriate copyright notice and 2 tells the user that there is no warranty for the work except to the extent that warranties are provided that licensees may covey the work under this License and how to view a copy of this License If the interface presents a list of user commands or options such as a menu a prominent item in the list meets this criteria 1 Source Code The source code for a work means the preferred form of the work for making modifications to it Object code means any non source form of a work A Standard Interface means an interface that either is an official standard defined by a recognized standards bo
47. ent ways but it always has the Start Button at the left and the Stop Button at the right Start Button Figure 2 12 Start Button The Start Button begins a run with the current settings Use it only after you have set the parameters for the run Stop Button Figure 2 13 Stop Button The Stop button stops the current run immediately This is ordinarily only used for emergencies or if you mistakenly set too long a run time 2 4 PN BO4155AC PN B04155AC The Touch Screen Interface 2 Areas on the Screen Footer Bar on the Home Page On the Home Page the middle of the Footer Bar shows three Items e The Vacuum Display Button e The System Name e The Accel Display Decel Display Button Figure 2 14 Footer Bar Home Page Open Vent Each of these is explained below Footer Bar on Other Pages When not on the Home Page the three buttons for Speed Time and Temperature appear in the Footer Bar for easy access To set Acceleration or Deceleration profiles or to use the Vacuum button simply press Home button to return to the Home Page The following items are described in detail within the Home Page section of the next chapter e The Set Speed Display Button The Set Time Display Button The Set Temp Display Button Figure 2 15 Footer Bar on Other Pages i Set Speed 50 000 RPM Set Time Hoki Set Temp 25 C E Vacuum Display Button As a display the Vacuum Display Button shows the current chamber vacuum and t
48. er Bar Running Status 2 3 2 8 Header Bar Stopping Status 2 3 2 9 Header Bar Warning Message 2 3 2 10 Header Bar Fatal Error Message 2 3 21i Help Button 2 4 2 12 Start Button 2 4 213 Stop Button 2 4 2 14 Footer Bar Home Page 2 5 2 15 Footer Bar on Other Pages 2 5 2 16 Item Help Button 2 6 2 17 Global Help Button attached to Help Button 2 7 2 18 Home Page Help with ghost image 2 7 219 Transparency Button 2 7 2 20 Home Page Help with opaque screen 2 8 2 21 Help Navigation Buttons 2 8 2 22 Zonal Continuous Flow Operation Help with scroll bar 2 9 3 1 Home Page 3 2 2 Zonal Operation Page with Cancel Button 3 3 3 3 Set Speed Page 3 3 3 4 Select Rotor and Labware Page 3 4 ERS Set Time Page 3 5 3 6 Set Temperature Page 3 6 cd Start Button 3 7 3 8 Zonal Page 3 8 3 9 Continuous Flow Page 3 9 4 1 Home Page 4 2 4 2 w t Display 4 2 xxii 4 3 4 4 4 5 4 6 4 7 4 8 4 9 4 10 4 11 4 12 4 13 4 14 4 15 4 16 4 17 4 18 4 19 4 20 4 21 4 22 4 23 4 24 4 25 4 26 4 27 4 28 4 29 4 30 4 31 5 1 Bia 5 3 Al A 2 Illustrations Set Speed Display Button 4 3 Set Time Display Button 4 3 Set Temp Display Button 4 3 Menu Page 4 4 Set Speed Page No Rotor Selected 4 5 Set Speed Page Rotor Selected 4 5 Set Time Page 4 6 Set Speed Time Page wt Mode 4 7 Set Temperature Page 4 8 Set Acceleration Deceleration Profiles Page 4 9 Select Rotor and Labware Page 4 11 Reference
49. er is the Environmentally Friendly Use Period EFUP date and indicates the number of calendar years the product can be in operation Upon the expiration of the EFUP the product must be immediately recycled The circling arrows indicate the product is recyclable The date code on the label or product indicates the date of manufacture China RoHS Environmental Label This label indicates that the electronic information product does not contain any toxic or hazardous substances The center e indicates the product is environmentally safe and does not have an Environmentally Friendly Use Period EFUP date Therefore it can safely be used indefinitely The circling arrows indicate the product is recyclable The date code on the label or product indicates the date of manufacture PN B04155AC xi Safety and Notices Summary of Instrument Labels Summary of Instrument Labels This section provides information for some labels and symbols appearing on the Optima XE instrument housing These labels and symbols may be associated with user serviceable procedures Individual hazards associated with a specific procedure in this manual may use these labels and symbols and are included in Warnings or Cautions within the procedures for that task Caution Symbol This symbol indicates a caution message and appears adjacent to an explanation or other symbols that define the caution Universal Serial Bus USB i This symbol indicates the
50. failed during a run and the rotor has stopped when power is restored During a Run If a power failure occurs during a run the rotor begins to decelerate with the brake off The corrected run time is determined when power is restored and if the set run time has not elapsed the run will resume as described Note that a rotor decelerating without the brake may take hours to come to a complete stop Rotor Spinning at Restoration If the rotor is still spinning when power is restored the instrument takes the following steps e Return to the set speed e Resume incrementing the run timer Set a diagnostic message to alert you that a power failure occurred during the run PN B04155AC 5 3 5 Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure Rotor Stopped at Restoration If the rotor has stopped spinning when power is restored the instrument cancels the run and sends a diagnostic message to alert you to that the run was cancelled due to a power failure Getting Access to the Sample If a power failure lasts for several hours you may have to remove the sample from the rotor while there is no power to the instrument The procedure requires removing the front panel which should be done only by qualified service personnel Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and or physical injury If any such procedure becomes necessary tur
51. fer to Chemical Resistances publication IN 175 or contact Beckman Coulter Field Service to ensure that the decontamination method does not damage any part of the instrument or accessories 5 2 PN B04155AC Maintenance and Troubleshooting Sterilization and Disinfection Sterilization and Disinfection While Beckman Coulter has tested these methods and found that they do not damage the instrument no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods The top working surface is finished with urethane paint The sides are finished with general purpose paint You can use Ethanol 70 on both these surfaces Ethanol is a volatile liquid that cannot be used on or near an operating instrument due to fire hazard Diagnostics User Messages When a condition arises that requires operator attention the windshield turns yellow or red A dialog will automatically open showing the diagnostic message User messages communicate information about the ultracentrifuge or alert you to an abnormal condition For a list of the possible malfunctions and their corrective actions see APPENDIX C Diagnostics Retrieving Your Sample in Case of Power Failure The instrument responds to a power failure during operations in two different ways e Power failed during a run and the rotor is still spinning when power is restored e Power
52. ge 3 4 PN B04155AC Operations 3 Manual Operation Step 3 Set the Time Press the Set Time Display Button to go to the Set Time Page Figure 3 5 Set Time Page Set Time H Wai Set Speed 15 000 RPM Set Time Hold Set Temp 25 C B On the Set Time Page follow two steps 1 Use the keypad to set the desired time in hours and minutes Use the Backspace and Clear keys to make corrections You can also use the Hold key to set the time to a hold state with no countdown to an automatic end When you use a Hold value the run does not end until you select the Stop key or until the maximum time of 999 hours and 59 minutes has been reached 2 Select the OK key to accept your entry and dismiss the page a PN B04155AC 3 5 Operations Manual Operation Step 4 Set the Temperature Press the Set Temp Display Button to go to the Set Temperature Page Figure 3 6 Set Temperature Page Set Temperature On the Set Temperature Page follow two steps 1 Use the keypad to set the desired temperature in degrees Celsius Use the Backspace and Clear keys to make corrections 2 select the OK key to accept your entry and dismiss the page a 3 6 PN B04155AC Operations 3 Zonal and Continuous Flow Operation Step 5 Start the Run Once the run values are set start the run 5 IN Prepare your samples and place them in the rotor following all proper procedures including balanced wei
53. ge Continuous Flow Authorization Page Service Login Page About Page Rotor Catalog Page Labware Catalog Page Export User Manual Page Set System Name Page Set Date and Time Page Set Sound Page Select Language Page Diagnostic History Page Compatible Tubes for Rotor Page Zonal Operation Page Continuous Flow Operation Page Running Home Page 4 1 Functional Pages Home Page Home Page The Home Page is the first operational page to appear after the instrument startup You return to it when you select the Home Page Button on the Header Bar It is the basic page from which you begin most operations In addition to the Header Bar and the Footer Bar the Home Page contains the following elements e The w t Display e The Set Speed Display Button The Set Time Display Button The Set Temp Display Button When you use Zonal Mode or Continuous Flow Mode the Zonal Continuous Flow Operation Page replaces the Home Page Figure 4 1 Home Page ot Display Figure 4 2 t Display The w t Display appears only when you have set the wt mode on the System Options Page It shows the accumuated t value for the run in progress See the Set Speed Time Page for details 4 2 PN BO4155AC Functional Pages 4 Home Page Set Speed Display Button Figure 4 3 Set Speed Display Button ad Set Speed 15 000 RPM The Set Speed Display Button shows the current rotor speed as a large number and also shows th
54. ght distribution Preheat or precool the rotor and samples if necessary Mount the rotor in the instrument following all the procedures in the rotor manual and observing all safety procedures and cautions Close and lock the chamber door For preconditioning press the Vacuum Display Button and wait until the chamber reaches the set temperature Press the Start button Figure 3 7 Start Button When the run begins wait for the countdown timer to reach zero and the rotor to come to a stop You can then remove your rotor and samples Zonal and Continuous Flow Operation In zonal and continuous flow operation the operator is unavoidably exposed to rotating machinery For safety the operator must be properly instructed and qualified Guard against accidentally dropping objects such as pens pencils or hemostats into the chamber Loose lab coats neckties scarves and long necklaces should not be worn while operating in the zonal or continuous flow mode Use only zonal rotors in the zonal mode Use only continuous flow rotors in the continuous flow mode Zonal and continuous flow operations require special rotors and have some additional hazards in that samples are loaded and unloaded while the rotor is spinning To prevent unauthorized users from attempting these operations the instrument requires a password which is 1793 When zonal PN B04155AC 3 7 Operations Zonal and Continuous Flow Operation or continuous flo
55. gure 2 16 Item Help Button 2 6 PN B04155AC The Touch Screen Interface J Help Messages Global Help The Global Help Button appears next to the Help Button If you select the Global Help Button the Page Display Area shows you a description of the current page and all the elements it contains Figure 2 17 Global Help Button attached to Help Button While using Global Help you also have some additional options e The Global Help page appears over a ghost image of the screen you were on when you selected global help Figure 2 18 Home Page Help with ghost image lost ly m Home Page E The Home Page is the first operations pag lo appear afier the instrument Startup You telum to when you touch the Home Page Header Bar Button on the Its the basc page from whch you begin most operations In additon to the Header Bar and the Footer Bar the Home Page contains the folowing slements The wt Display appears when the wt mode is active See the Set Speed Time Page for detais The Set Speed Display Button takes you lo the Set Speed Page The Set Time Display Button takes you to the Set Time Pane The Set Temp Display Button takes you lo the Set Temperature Page When you use Zonal Mode or Continuous Flow Mode the Zonal Continious Flow Operation Page replaces the Home Page Table of Contents If the ghost image makes it difficult for you to read the Help Page select the Transparency Button in the upper right corner Figu
56. h party to that transaction who receives a copy of the work also receives whatever licenses to the work the party s predecessor in interest had or could give under the previous paragraph plus a right to possession of the Corresponding Source of the work from the predecessor in interest if the predecessor has it or can get it with reasonable efforts You may not impose any further restrictions on the exercise of the rights granted or affirmed under this License For example you may not impose a license fee royalty or other charge for exercise of rights granted under this License and you may not initiate litigation including a cross claim or counterclaim in a lawsuit alleging that any patent claim is infringed by making using selling offering for sale or importing the Program or any portion of it 11 Patents A contributor is a copyright holder who authorizes use under this License of the Program or a work on which the Program is based The work thus licensed is called the contributor s contributor version A contributor s essential patent claims are all patent claims owned or controlled by the contributor whether already acquired or hereafter acquired that would be infringed by some manner permitted by this License of making using or selling its contributor version but do not include claims that world be infringed only as a consequence of further modification of the contributor version For purposes of this defin
57. he action that will be performed when the button is pressed As a control it serves two functions e Before a run after mounting the rotor and closing the chamber door press this control to evacuate the chamber and precondition the chamber to the set temperature After a run press this control to release the vacuum before opening the chamber door System Name The system name is simply a display You can set the system name as one of the options on the System Options Page 2 5 The Touch Screen Interface Help Messages Accel and Decel Display Buttons These are two displays that together act as a single button As a display each shows the selected acceleration or deceleration profile currently selected If you select either one of them it takes you to the Set Acceleration Deceleration Profiles Page described in the next chapter Page Display Area The Page Display Area is the main display area between the Header Bar and the Footer Bar The pages and help messages all appear in this area The pages are all described in CHAPTER 4 Help Messages When you first select the Help Button a number of new buttons appear on the screen giving you three sets of options Item Help e Global Help Exit from Help Item Help Item Help Buttons appear on screen items where you can get an item help message These are brief pop up messages describing what the item does Only one help message remains on the screen at a time Fi
58. hree ways When the timer countdown reaches zero the instrument decelerates to the unloading speed and goes to the Unloading phase e Ifyou select the Unload Button to terminate the run early the instrument decelerates to the unloading speed and goes to the Unloading phase e Ifyou select the Stop Button to abort the run the instrument brings the rotor to a halt skips the unloading phase and exits Zonal or Continuous Flow mode entirely Unloading the Sample While the rotor is running at unload speed follow the instructions in the rotor manual to inject the displacement solution into the rotor and collect the resulting centrifugate sample Stopping the Run When you have completed all the defined unloading steps press the Stop Button to bring the rotor to a halt Finishing the Run When the rotor comes to a halt unmount it and perform all the cleanup and follow up steps given in the rotor manual The instrument exits Zonal or Continuous Flow mode when the rotor comes to a stop 3 10 PN B04155AC _ PN B04155AC CHAPTER 4 Functional Pages This chapter describes the functional pages that you use to control the Optima XE Ultracentrifuge They include all of the following Home Page Menu Page Set Speed Page Set Time Page Set Speed Time Page wt Mode Set Temperature Page Set Acceleration Deceleration Profiles Page Select Rotor and Labware References Page System Options Page Zonal Authorization Pa
59. ic refrigeration and heating system without coolant or water Cooling is provided by forced air from the fans When the power is on the temperature control system starts when the door is closed and the vacuum system is running Drive The frequency controlled air cooled direct drive induction motor requires no gears or brushes In addition the drive does not require an oil vacuum seal external oil reservoir or continuously operating damper Externally cooled by forced air and internally cooled by oil the drive delivers ultra smooth quiet performance with high rotor imbalance tolerance 1 2 PN B04155AC Description Safety Features Safety Features Door The Optima XE ultracentrifuge has been designed and tested to operate safely indoors at altitudes up to 2000 m 6562 ft The high strength structural steel chamber door has a solenoid interlock to prevent operator contact with a spinning rotor When the door is closed and a run begins it locks automatically It can be opened only when the power is on and the rotor is at rest with the chamber at atmospheric pressure If there is a power failure you can manually unlock the door as described in the Maintenance and Troubleshooting chapter Barrier Ring A 41 mm 1 63 in steel alloy armor ring acts as the primary barrier surrounded by a 13 mm 0 5 in vacuum chamber to provide full protection for the operator Imbalance Detector An imbalance detector monitors the rotor d
60. ing the chamber at the beginning of a run or to release the vacuum at the end of arun e The Unload Speed Display shows the sample unloading speed Use the arrow buttons above and below to increase or decrease the sample unloading speed e The Cancel Button ends Zonal Mode before loading is complete and returns you to the Home Page Use the Footer Bar for setting the run speed time and temperature PN B04155AC 4 27 Functional Pages Continuous Flow Operation Page Continuous Flow Operation Page Figure 4 30 Continuous Flow Operation Page Press Start to go fo Load speed Load Speed Vacuum Unload Speed The Continuous Flow Operation Page appears when you enter the correct password and select the Authorize Button from the Continuous Flow Authorization Page putting the instrument in Continuous Flow mode When you use Continuous Flow mode the Continuous Flow Operation Page replaces the Home Page until Continuous Flow mode ends In addition to the Header Bar and the Footer Bar the Continuous Flow Operation Page contains the following elements The Status Display shows the current step in the Continuous Flow Run procedure See Zonal and Continuous Flow Operation in the Operations chapter for details The Loading Complete Button ends the sample loading step and proceeds to the running step The Unload Button terminates the running step early and proceeds to the sample unloading step The Load Speed Display shows the sa
61. is specifically designed to require such as by intimate data communication or control flow between those subprograms and other parts of the work The Corresponding Source need not include anything that users can regenerate automatically from other parts of the Corresponding Source The Corresponding Source for a work in source code form is that same work PN B04155AC Third Party Contributions and Legal Notices D 2 Basic Permissions All rights granted under this License are granted for the term of copyright on the Program and are irrevocable provided the stated conditions are met This License explicitly affirms your unlimited permission to run the unmodified Program The output from running a covered work is covered by this License only if the output given its content constitutes a covered work This license acknowledges yours rights of fair use or other equivalent as provided by copyright law You may make run and propagate covered works that you do not convey without conditions so long as your license otherwise remains in force You may convey covered works to others for the sole purpose of having them make modifications exclusively for you or provide you with facilities for running those works provided that you comply with the terms of this License in conveying all material for which you do not control copyright Those thus making or running the covered works for you must do so exclusively on your behalf under your direction and c
62. ition control includes the right to grant patent sublicenses in a manner consistent with the requirements of the License Each contributor grants you a non exclusive worldwide royalty free patent license under the contributor s essential patent claims to make use sell offer for sale import and otherwise run modify and propagate the contents of its contributor version In the following three paragraphs a patent license is any express agreement or commitment however denominated not to enforce a patent such as an express permission to practice a patent or covenant not to sue for patent infringement To grant such a patent license to a party means to make such an agreement or commitment not to enforce a patent against the party If you convey a covered work knowingly relying on a patent license and the Corresponding Source of the work is not available for anyone to copy free of charge and under the terms of this License through a publicly available network server or other readily accessible means then you must either 1 cause the Corresponding Source to be so available or 2 arrange to deprive yourself of the benefit of the patent license for this particular work or 3 arrange in a manner consistent with the PN B04155AC D 9 Third Party Contributions and Legal Notices D 10 requirements of this License to extend the patent license to downstream recipients Knowingly relying means you have actual knowle
63. line and load have not been evaluated to be back fed Locate the ultracentrifuge in a clean safe uncluttered environment free of volatile vapors that could be ignited by the operation of the centrifuge PN B04155AC A 1 Preinstallation Requirements Space Requirements Do not place the ultracentrifuge near areas containing flammable reagents or combustible fluids Vapors from these materials could enter the ultracentrifuge air system and be ignited by the motor Although the possibility of a rotor failure is remote your planning should include a safety space around the instrument of 12 inches 30 5 cm to allow for the movement of the instrument in the event that a rotor failure occurs at high rotational speed If you decide to install the instrument in an area where furniture equipment or a wall is within this safety space you run the risk of damage to anything within this space in the event of a rotor failure Maintain a 30 cm 1 ft clearance envelope around the ultracentrifuge while it is running No persons or any hazardous materials should be within this clearance boundary while the ultracentrifuge is operating except to change operating controls if required Figure A 1 Safety and ventilation space 30 5 om 12 in Ventilation If you choose to install the instrument with less than the 12 in 30 5 cm safety clearence you must at least provide a required ventilation and service access clearence of 6 i
64. ll Outlet NEMA 6 30 R 2 Measured Line Voltage 5 Earth Ground 3 North American Plug 6 30 ampere Circuit Protector PN B04155AC A 3 Preinstallation Requirements Electrical Requirements A 4 To ensure safety the instrument should be wired to a remote emergency switch preferably outside the room where the ultracentrifuge is housed or adjacent to the exit from that room Refer to Table A 1 Table A 1 Required Wire Connections Wire Insulation Color Terminal Symbol Harmonized North American SN HAN Green Yellow Earth ground Light Blue Neutral N L Brown Live or Line L L PN B04155AC APPENDIX B Special Warranty for the Optima XE Special Warranty PN B04155AC Subject to the exceptions and upon the conditions specified below Beckman Coulter Inc agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within one 1 year after delivery of the Optima Ultracentrifuge the product to the original Buyer by Beckman Coulter or by an authorized representative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use Some components and accessories by their nature are not intended to and will not function for as long as one 1 year If any such component or accessory fails to give reasonable service for a reasonable period
65. location of a universal serial bus USB connector Ethernet Jf This symbol indicates the location of an ethernet connector Biohazard This symbol indicates a biohazard xii PN B04155AC Safety and Notices Summary of Instrument Labels Recycling Label A A A28219 AA Si This symbol is required in accordance with the Waste Electrical and Electronic Equipment WEEE Directive of the European Union The presence of this marking on the product indicates e the device was put on the European Market after August 13 2005 and e the device is not to be disposed of via the municipal waste collection system of any member state of the European Union It is very important that customers understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection treatment recovery recycling and safe disposal of the device Multiple Compliance amp N395 169502 CE AZ B00942 AA This symbol indicates compliance with IVD For in vitro diagnostic use e N395 The C Tick mark is intended for use on products that comply with Australian Communication Authority ACA EMC Requirements 169502 This label indicates recognition by a Nationally Recognized Testing Laboratory NRTL that the i
66. man Coulter and serviced only by Beckman Coulter Field Service representatives If the above conditions are not met the full appropriate exchange price for the drive will be charged Disclaimer IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT PN B04155AC APPENDIX C Diagnostics Overview This section lists possible malfunctions and corrective actions Maintenance procedures are described in Chapter 5 Maintenance and Troubleshooting For any maintenance not covered in this manual contact Beckman Coulter Field Service for assistance USA customers can call 1 800 742 2345 For international contacts see the website at www beckmancoulter com or use the contact numbers on the inside front cover of this book NOTE Itis your responsibility to decontaminate the ultracentrifuge as well as any rotors and or accessories before requesting service by Beckman Coulter Field Service Diagnostics User Messages Chart Refer to the chart below for a list of possible malfunctions and their corrective actions Table C 1 Diagnostics User Messages Chart Message Definition Result Recommended Action D100 Turn off power Instrument ca
67. ment In addition to the Header Bar and the Footer Bar the About Page contains the following operating elements e The Done button dismisses the page The Export button takes you to the export About Page NOTE Do not remove the USB drive while a data transfer is in progress e The Available Drives list shows all the available USB drives If the list is empty the prompt Insert USB Drive appears below the list area Select an available USB drive to receive the information e The Export Button transfers the information to the selected USB drive PN B04155AC 4 17 4 Functional Pages Rotor Catalog Page Rotor Catalog Page 4 18 Figure 4 20 Rotor Catalog Page Rotor Catalog Max Speed 100 000 RPM Type 10071 LS K Factor 15 1 psani E Maximum RCF 801 900 xg Type 70 TI Type 70 1 TI RCF Rmin 442 400 xg Tube Capacity 6 0 mL Total Capacity 48 0 mL RCF Ravg 622 200 x g Type 50 2 Ti Type 50 4 Ti Tube Count 8 predam l Tube Angle 26 0 RS Set Speed 15 000 RPM Set Time 0 05 h mm Set Temp 25 C E The Rotor Catalog Page appears when you select the Rotor Catalog Button on the References Page allowing you to examine the detailed specifications for all the rotors available for your instrument This is different from the Select Rotor and Labware Page where you choose a rotor from the Set Speed Page In addition to the Header Bar and the Footer Bar the Rotor Catalog Page contain
68. mple loading speed Use the arrow buttons above and below to increase or decrease the sample loading speed The Vacuum Display Button shows the current vacuum Press it to begin evacuating and preconditioning the chamber at the beginning of a run or to release the vacuum at the end of arun The Unload Speed Display shows the sample unloading speed Use the arrow buttons above and below to increase or decrease the sample unloading speed The Slow to Zero rpm Button is for preliminary steps which require bringing the rotor to a stop temporarily during the initial loading adjustments The Cancel Button ends Continuous Flow Mode before loading is complete and returns you to the Home Page Use the Footer Bar for setting the run speed time and temperature 4 28 PN B04155AC Functional Pages 4 Running Home Page Running Home Page PN B04155AC Figure 4 31 Running Home Page Running Set Speed 50 000 RPM Set Time 0 01 h mm Set Temp 25 C The Running Home Page appears when there is an actual run in progress In addition to the Header Bar and the Footer Bar the Running Home Page contains the following elements e The Set Speed Display shows the current Set Speed progress of the run in RPM or RCF The last two digits are always zeroes when the speed is above 1000 RPM e The Set Time Display shows the current Set Time progress of the run in hours and minutes e The Set Temperature Display shows the current Set
69. n 15 2 cm behind the instrument and 2 in 5 1 cm on each side In addition the ultracentrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during operation Temperature The instrument operates within specifications in a laboratory with ambient temperatures ranging from 10 to 35 C A 2 PN BO4155AC Preinstallation Requirements A Electrical Requirements Electrical Requirements Instrument Rating 200 to 240 VAC 50 60 Hz 20 A Power Line Range Input Ratings 180 to 264 VAC 60 or 50 Hz single phase 30 A Power Line Range Output Ratings 200 to 240 VAC 50 60Hz 8 5A IMPORTANT AC output is not bonded to earth ground To reduce risk of electrical shock this equipment uses a three wire electrical cord 3 05 m 10 ft and plug see Figure A 2 to connect the equipment to earth ground In regions where the instrument is supplied with an unterminated cord a plug that meets local electrical and safety requirements must be supplied Contact your local Beckman Coulter office for specific information regarding these requirements See Table A 1 for the required wire connections Make sure that the matching wall outlet is located near the centrifuge and is easily accessible properly wired and earth grounded NOTE The power plug serves as the Disconnecting Device and must remain easily accessible Figure A 2 Electrical Connection 1 30 ampere Circuit Protector 4 Wa
70. n the power switch OFF and then disconnect the instrument from the main power source by removing its power plug from the receptacle Refer the maintenance to qualified service personnel To get access to the rotor follow these steps e Disconnect the power by removing its power plug from the receptacle e Remove the front panel e Vent the chamber to release the vacuum Release the door lock Open the door The following procedure should be performed only when absolutely necessary and only by qualified service personnel Check Power Check that the power switch is in the OFF position and the instrument is disconnected from the power i e the power plug is removed from its receptacle Remove the Front Panel To remove the front panel 1 Locate the three front panel latch screws and use a small flat head screwdriver to turn each latch screw counter clockwise until each one is loose Refer to Figure 5 1 5 4 PN B04155AC Maintenance and Troubleshooting 5 Retrieving Your Sample in Case of Power Failure Figure 5 1 Panel screws and Tabs 1 Panel Tabs 3 Front Panel 2 Top Panel 4 Latch Screws 2 Whenall three latch screws are loose use your hand to lift the top panel until it pops loose 3 Lift the front panel until it is free and tilt the top edge towards you 4 Lift the front panel off the tabs that secure it at the bottom and put it ina safe place PN B04155AC 5 5 Maintenance and Troubleshooting
71. nd Notices Safety Notices Safety Notices Before installing using or maintaining the instrument be certain that you know all the following precautions Information Read all product manuals and consult with Beckman Coulter trained personnel before attempting to operate instrument Do not attempt to perform any procedure before carefully reading all instructions Always follow product labeling and manufacturer s recommendations If in doubt as to how to proceed in any situation contact your Beckman Coulter Representative Installation This instrument is designed to be installed by a Beckman Coulter Field Service representative Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument Also should the instrument need to be moved a Beckman Coulter Field Service representative must reinstall and re level the instrument in its new location Do not place the ultracentrifuge near areas containing flammable or combustible fluids or any other source of vapors that could enter the ultracentrifuge air system and be ignited by the motor Ultracentrifuge operations generate high energy levels that require precautions against sudden movements that could result from the rare event of a rotor failure Maintain a 30 cm 1 ft clearance envelope around and above the ultracentrifuge Do not install any equipment or furniture in this envelope While the ultracentrifuge is running keep
72. nnot trip breaker Call Beckman Coulter Field Service immediately D101 SBC Single board computer boot Call Beckman Coulter Field Service Communications error D102 SCB Communications error Call Beckman Coulter Field Service Communications D103 Inverter Inverter C error Call Beckman Coulter Field Service Communications D104 Inverter Inverter ADC C error U21 Call Beckman Coulter Field Service Communications D105 Inverter Inverter I C 1 0 Expander error Call Beckman Coulter Field Service Communications PN B04155AC C 1 Diagnostics Diagnostics User Messages Chart Table C 1 Diagnostics User Messages Chart Continued Message Definition Result Recommended Action D107 Inverter Communications TEM ADC C error U24 Call Beckman Coulter Field Service D108 Network Communications D108 Network Communications Be sure external devices are turned on D109 Network Communications Network error ethernet disconnected Be sure network cable is plugged in D110 Network Communications Network error cannot renew IP address Check to see if network is active D111 System Data Error Data error Note If recovery fails this condition will result in a 200 minute delay before the diagnostic can be cleared and the door opened The power must be left on until the delay period is completed Call Beckman Coulter Field Se
73. nostic History Page Diagnostic History Page PN B04155AC Diagnostic History Total Logs 6 0203 4 7 2011 2 40 PM D203 Bus voltage Date amp Time 4 7 2011 2 40 PM 4 7 2011 2 39 PM A117 EM sd SAMORA Set Time Hold 4 5 2011 3 16 PM D123 Set Temp 25 C Acceleration Maximum 4 5 2011 3 10 PM D101 Deceleration Maximum Actual Speed ORPM 4 5 2011 3 09 PM A120 Actual Time 002 Actual Temp 25 0 C 4 5 2011 3 08 PM A117 Vacuum 0pm Run User Administrator Current User Administrator Rotor Type No Rotor Rotor Serial Number Set Time 0 05 h mm Set Temp 25 C The Diagnostic History Page appears when you select the Diagnostic History Button on the System Options Page allowing you to examine and export the details of incidents that caused a diagnostic message on the instrument warnings and error messages In addition to the Header Bar and the Footer Bar the Diagnostic History Page contains the following elements e The List of Events on the left side of the screen shows all the diagnostic events for the instrument Scroll through this list and select an event to view the detailed information about it e The Event Details on the right side of the screen shows all the details for the selected event on the List of Events e The Back Button returns you to the System Options Page e The Export Button takes you to the Export Diagnostic Page to let you copy the diagnostic history to a USB drive NOTE Do not remove the
74. nstrument has met the relevant product safety standards e CE This label indicates conformance to various Directives set forth under European Union law e Recycling Refer to the Recycling Label section in this document PN B04155AC xiii Safety and Notices Summary of Instrument Labels CAUTION Static Sensitive Area Indicates an area of the instrument which is sensitive to static electrostatic discharge ESD To prevent damage due to electrostatic discharge always wear a properly earth grounded wrist strap while operating this instrument For details on proper grounding see IEEE standard P1100 DANGER High Voltage DANGER HIGH VOLTAGE HAUTE TENSION Operation replacement or servicing of any components where contact with bare live hazardous parts could occur possibly resulting in electric shock should only be performed by your Beckman Coulter representative Protective Ground This symbol is used to indicate a protective ground This instrument must be properly grounded Do not under any circumstances operate the instrument unless it is properly grounded Alternating Current This symbol is used to indicate an alternating current also known as AC xiv PN B04155AC Safety and Notices Summary of Instrument Labels Output Plate Label Utility Interactive Output 200 240 VAC 50 60 Hz 8 5A 0 99pf Max Output Power 1 7kW Max Output Fault Current 20A Max Ambient 35C This unit or system is
75. nty or assumption of liability accompanies a copy of the Program in return for a fee END OF TERMS AND CONDITIONS PN BO4155AC D 11 Third Party Contributions and Legal Notices PN B04155AC www beckmancoulter com secs COULTER 2015 Beckman Coulter Inc All Rights Reserved
76. oes not terminate the licenses of parties who have received copies or rights from you under this License If your rights have been terminated and not PN B04155AC Third Party Contributions and Legal Notices D permanently reinstated you do not qualify to receive new licenses for the same material under section 10 9 Acceptance Not Required for Having Copies Your are not required to accept this License in order to receive or run a copy of the Program Ancillary propagation of a covered work occurring solely as a consequence of using peer to peer transmission to receive a copy likewise does not require acceptance However nothing other than this License grants you permission to propagate or modify any covered work These actions infringe copyright if you do not accept this License Therefore by modifying or propagating a covered work you indicate your acceptance of this License to do so 10 Automatic Licensing of Downstream Recipients Each time you convey a covered work the recipient automatically receives a license from the original licensors to run modify and propagate that work subject to this License You are not responsible for enforcing compliance by third parties with this License An entity transaction is a transaction transferring control of an organization or substantially all assets of one or subdividing an organization or merging organizations If propagation of a covered work results from an entity transaction eac
77. of time Beckman Coulter will repair or at its election replace such component or accessory What constitutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter Replacement Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer with the transportation charges collect unless the product is found to be defective in which case Beckman Coulter will pay all transportation charges Beckman Coulter makes no warranty concerning products or accessories not manufactured by it In the event of failure of any such product or accessory Beckman Coulter will give reasonable assistance to the Buyer in obtaining from the respective manufacturer whatever adjustment is reasonable in light of the manufacturer s own warranty Damage to the instrument while operating a rotor not of Beckman Coulter manufacture is not covered by warranty or service contract terms Further Beckman Coulter shall be released from all obligations under all warranties either expressed or implied if the product covered hereby is repaired or modified by persons other than its own authorized service personnel unless such repair is made by others who meet qualifications similar to those required of Beckman Coulter s service personnel or unless such repair in the sole opinion of Beckman Coulter is minor or unless such modificati
78. on in the Header Bar allowing you access to the menu choices Choosing an option dismisses the Menu page and goes to the selected page e The Options Button takes you to the System Options Page e The References Button takes you to the References Page e The About Button takes you to the About Page e The Zonal Operation Button takes you to the Zonal Operation Page e The Continuous Flow Operation Button takes you to the Continuous Flow Authorization Page e The Service Mode Button is used by service personnel only If you accidently select this button and go to the Service Login Page select the Cancel button to dismiss the page Each of these pages is described in a following section There is also a Done button at the bottom of the page that dismisses the page 4 4 PN BO4155AC Set Speed Page PN B04155AC Figure 4 7 Set Speed Page No Rotor Selected Ar e Ready Set Speed 50 000 Set Speed 15 000 Type 100 Ti 5 6 mL Quick Seal 344619 Functional Pages 4 Set Speed Page The Set Speed Page appears when you select the Set Speed Display Button on the Home Page or the Footer Bar allowing you to set the speed for the next run As an alternate if you have already selected a rotor you can set the units in RCF Relative Centrifugal Field instead of RPM If the instrument is in t mode the Set Speed Time Page appears instead In addition to the Header Bar and the Footer Bar the Set Speed Page contains
79. on is merely the installation of a new Beckman Coulter plug in component for such product B 1 Special Warranty for the Optima XE Special Warranty B 2 Special Drive Warranty During the instrument warranty period one year there will be no charge for drive replacement if the drive unit is installed serviced and operated in accordance with the conditions listed below During the drive s second through tenth year of use there is a prorated drive replacement price based on years of use if the drive unit is installed serviced and operated in accordance with the conditions listed below Drive replacement price for units not under service contract current drive exchange price ears of use x ZES or use ag 10 labor and travel NOTE For details of drive coverage with a service contract contact your local Beckman Coulter service representative Conditions 1 The drive has been operated only within its rated speed and temperature ranges 2 The drive unit has not been subjected to unequal loading improper rotor installation corrosion from material spilled onto the hub or accumulated in the chamber of the instrument 3 The drive unit has not been disassembled modified or repaired except by Beckman Coulter personnel The drive unit was installed by a Beckman Coulter Field Service representative The instrument in which the drive unit has been used and operated and its associated rotors were manufactured by Beck
80. ontrol on terms that prohibit them from making any copies of your copyrighted material outside their relationship with you Conveying under any other circumstances is permitted solely under the conditions stated below Sublicensing is not allowed section 10 makes it unnecessary 3 Protecting Users Legal Rights From Anti Circumvention Law No covered work shall be deemed part of an effective technological measure under any applicable law fulfilling obligations under article 11 of the WIPO copyright treaty adopted on 20 December 1996 or similar laws prohibiting or restricting circumvention of such measures When your convey a covered work you waive any legal power to forbid circumvention of technological measures to the extent such circumvention is effected by exercising rights under this License with respect to the covered work and you disclaim any intention to limit operation or modification of the work as a means of enforcing against the work s users your or third parties legal rights to forbid circumvention of technological measures 4 Conveying Verbatim Copies You may convey verbatim copies of the Program s source code as you receive it in any medium provided that you conspicuously and appropriately publish on each copy an appropriate copyright notice keep intact all notices stating that this License and any non permissive terms added in accord with section 7 apply to the code keep intact all notices of the absence of any war
81. ore you have certain responsibilities if you distribute copies of the software or if you modify it responsibilities to respect the freedom of others For example if you distribute copies of such a program whether gratis or for a fee you must pass on to the recipients the same freedoms that you received You must make sure that they too receive or can get the source code And you must show them these terms so they know their rights Developers that use the GNU GPL protect your rights with two steps 1 assert copyright on the software and 2 offer you this License giving you legal permission to copy distribute and or modify it For the developers and authors protection the GPL clearly explains that there is no warranty for this free software For both users and authors sake the GPL requires that modified versions be marked as changed so that their problems will not be attributed erroneously to authors of previous versions Some devices are designed to deny users access to install or run modified versions of the software inside them although the manufacturer can do so This is fundamentally incompatible with the aim of protecting users freedom to change the software The systematic pattern of such abuse occurs in the area of products for individuals to use which is precisely where it is most unacceptable Therefore we have designed this version of the GPL to prohibit the practice for those products If such problems aris
82. ossibility that the rotor is still spinning If it is still spinning you can hear a whining noise when you begin to vent the chamber If you hear the whining noise when you begin to vent the chamber you must close the port immediately and wait for the rotor to come to a stop The following steps describe the procedure you must follow for each configuration To Vent the Chamber Configuration A with vent port cap 1 Locate the vacuum vent port cap on the side of the rotor chamber 2 Turnthe cap very slowly counter clockwise until you can hear the hiss of air entering the chamber 3 Ifyou hear a whining noise immediately turn the cap clockwise to close it and wait until the rotor has had time to come to a stop at least an hour before attempting to vent the chamber again 4 When air flows into the chamber without a whining noise remove the cap completely N To Vent the Chamber Configuration B with solenoid release screw 1 Locate the vacuum solenoid release screw on the side of the rotor chamber 2 Using a screwdriver turn the screw very slowly clockwise until you can hear the hiss of air entering the chamber 3 Ifyou hear a whining noise immediately turn the screw counter clockwise to close it and wait until the rotor has had time to come to a stop at least an hour before attempting to vent the chamber again When air flows into the chamber without a whining noise continue to turn the screw clockwise
83. ow Mode you have to press the Cancel Button to go to the Home Page 3 2 PN B04155AC Operations 3 Manual Operation Figure 3 2 Zonal Operation Page with Cancel Button Running z Unloading St selon Stopping Loading Complete Unioad Load Speed Vacuum Unload Speed Step 2 Set the Speed and Rotor Press the Set Speed Display Button on the Home Page to go to the Set Speed Page Figure 3 3 Set Speed Page Set Speed 50 000 Set Speed 50 000 RPM PN B04155AC 3 3 Operations Manual Operation When the Set Speed Page appears follow these steps 1 Press the Select Rotor Button to go to the Select Rotor and Labware Page Figure 3 4 Select Rotor and Labware Page Select Rotor and Labware a Volume Type P N Type 100 Ti p No Tube Type 90 TI Type 70 Ti Type 70 1 Ti Type 50 4 Ti Type 50 2 TI Type 45 Ti Set Speed 50 000 RPM 2 Select the rotor and labware for the run from the list 3 Select the OK Button to return to the Set Speed Page 4 if you plan to set the speed in units of relative centrifugal field RCF select the RPM RCF Button Note that the RPM RCF button is only enabled when you have selected a rotor 5 use the keypad to set the desired speed Note that you actually enter hundreds with two ending zeroes added to your number You can also use the Backspace and Clear keys to make corrections 6 select the OK key to accept your entry and dismiss the pa
84. owing elements e The Authorize Display allows you to enter the authorization password which is 1793 For security reasons the display shows bullets in place of the numbers you enter The Keypad for entering the password e The Cancel Button dismisses the page without entering Zonal mode e The Authorize Button accepts the password you have entered and if the password is the correct one takes you to the Zonal Operation Page which replaces the Home Page while you are using Zonal mode 4 14 PN BO4155AC Functional Pages Continuous Flow Authorization Page Continuous Flow Authorization Page Figure 4 17 Continuous Flow Authorization Page Continuous Flow Authorization Authorize eeeese Set Speed 50 000 RPM Set Time Hold The Continuous Flow Authorization Page appears when you select the Continuous Flow Operation Button on the Menu Page allowing you to use Continuous Flow mode for the next run In addition to the Header Bar and the Footer Bar the Continuous Flow Authorization Page contains the following elements e The Authorize Display allows you to enter the authorization password which is 1793 For security reasons the display shows bullets in place of the numbers you enter e The Keypad for entering the password The Cancel Button dismisses the page without entering Continuous Flow mode e The Authorize Button accepts the password you have entered and if the password is the correct one takes you to
85. peed Signals Overspeed timing signal is lt 9 counts revolution or gt 47 counts revolution or overspeed timing signal is unstable 1 Check the condition of the rotor overspeed disk 2 Make sure the rotor is installed properly 3 Verify the rotor load is within limits specified in the rotor manual D304 Speed Signals No tachometer signal NOTE This condition will result in a 200 minute delay before the diagnostic can be cleared and the door opened The power must be left on until the delay period is completed 1 Make sure the rotor is installed properly 2 Check the condition of the rotor overspeed disk 3 Verify the rotor load is within limits specified in the rotor manual 4 Ifthe problem persists call Beckman Coulter Field Service D305 Inertia Inertia check failure Be sure rotor is loaded properly C 4 PN B04155AC PN B04155AC Table C 1 Diagnostics User Messages Chart Continued Diagnostics Diagnostics User Messages Chart Message Definition Result Recommended Action A400 Alert Vacuum Calibration Vacuum calibration failure Call Beckman Coulter Field Service A401 Alert Slow Vacuum Vacuum exceeds 750 microns after 4 minutes 1 Make sure door o ring is clean undamaged and properly lubricated 2 Check for sample leakage Clean and dry the rotor chamber if needed 3 If the problem persists call Beckman Coulter Field Ser
86. peed Time Page wt Mode Set Speed Time Page wt Mode Figure 4 10 Set Speed Time Page wt Mode Set Speed Time wit 6 5797e5 Type 100 Ti 5 6 mL Quick Seal 344619 Set Speed 15 000 RPM The Set Speed Time Page appears when the w t mode is active and you select either the Set Speed Button or the Set Time Button on the Home Page or the Footer Bar The wt value is computed from the time and RPM or RCF values you enter Having these all on the same page allows you to set the values for the next run that produce the desired wt value Turn the wt mode on and off with the mt Mode Button on the System Options Page In addition to the Header Bar and the Footer Bar the Set Speed Time Page contains the following elements e The Set Speed Button Display shows the current Speed in RPM or RCF When you touch it the keyboard becomes active to set a new speed value The t value changes to reflect the new speed value The w t Button Display shows the current wt value When you touch it the keyboard becomes active to set a new t value The time value changes to reflect the new wt value e The Set Time Button Display shows the current time setting in hours and minutes When you touch it the keyboard becomes active to set a new time value The wt value changes to reflect the new time value The RPM RCF Button selects the type of speed units You must have a rotor selected to use RCF e The Select Rotor Button takes
87. product that is covered by this License on a durable physical medium customarily used for software interchange for a price no more than your reasonable cost of physically performing this conveying of source or 2 access to copy the Corresponding Source from a network server at no charge c Convey individual copies of the object code with a copy of the written offer to provide the Corresponding Source This alternative is allowed only occasionally and noncommercially and only if you received the object code with such an offer in accord with subsection 6b d Convey the object code by offering access from a designated place gratis or for a charge and offer equivalent access to the Corresponding Source in the same way through the same place at no further charge You need not require recipients to copy the Corresponding Source along with the object code If the place to copy the object code is a network server the Corresponding Source may be on a different server operated by you or a third party that supports equivalent copying facilities provided you maintain clear directions next to the object code saying where to find the Corresponding Source Regardless of what server hosts the Corresponding Source you remain obligated to ensure that it is available for as long as needed to satisfy these requirements E Convey the object code using peer to peer transmission provided you inform other peers where the object code and Corresponding Source of
88. provided with fixed trip limits and shall not be aggregated above 30kW on a single Point of Common Connection This is the label that indicates what the output rating are on the instrument Rotor Rotation This indicates the direction of instrument rotor rotation PN B04155AC XV Safety and Notices Symbols Symbols Symbol Simbolo Symbol E Symbole 74 6 Title Titel Titre Titulo Titolo BFR ARR Simbolo Dangerous voltage Gefahrliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo alta tensione repeat Fs a HA Fis Attention consult accompanying documents Achtung Begleitpapiere beachten Attention consulter les documents joints Atencidn consulte los documentos adjuntos Attenzione consultare le informazioni allegate RE ABA SBOE ER HERA H On power Ein Netzverbindung Marche mise sous tension Encendido Acceso sotto tensione A SH FF CHW Off power Aus Netzverbindung Arr t mise hors tension Apagado Spento fuori tensione D SR K CHB Protective earth ground SchutzleiteranschluB Liaison a la terre Puesta a tierra de protecci n Collegamento di protezione a terra REP A FEL tea Best Earth ground Erde Terre Tierra Scarica a terra 7 2 Ei Beth Alternating Current Wechselstrom Courant alternatif Corriente Alterna Corrente Alternata prdi tS xvi PN B04155AC Contents E Revision Histo
89. r to release the vacuum at the end of a run The Unload Speed Display shows the sample unloading speed Use the arrow bullons above and below lo raise or lower the sample unloading speed 2 9 The Touch Screen Interface Help Messages 2 10 PN B04155AC CHAPTER 3 Operations _ The touch screen interface for the Optima XE makes operations about as simple as possible Although there are many additional things you can do this chapter outlines basic operations e Manual Operation Operations with Preconditioning Continuous Flow Operation e Zonal Operation Manual Operation Manual operation is a simple procedure you can do from the Home Page Before you begin there are four things you must know e What kind of rotor you are using for the run e What speed you want for the run e How long the run should be e What temperature you want for the run When you have this information the procedure for the run is as follows 1 start on the Home Page 2 Set the speed and rotor 3 Set the time 4 set the temperature PN B04155AC 3 1 Operations Manual Operation 5 Start the run Step 1 Start on the Home Page Start at the Home Page If your screen shows any other page press the Home Page Button in the upper left corner of the screen Figure 3 1 Home Page Sel Speed 100 000 RPM Set Time 0 01 h mm Set Temp 25 C NOTE If your system is in Zonal Mode or Continuous Fl
90. ranty and give all recipients a copy of this License along with the Program You may charge any price or no price for each copy that you convey and you may offer support or warranty protection for a fee 5 Conveying Modified Source Versions You may convey a work based on the Program or the modifications to produce it from the Program in the form of source code under the terms of section 4 provided that you also meet all of these conditions a The work must carry prominent notices stating that you modified it and giving a relevant date b The work must carry prominent notices stating that it is released under this License and any conditions added under section 7 This requirement modifies the requirement in section 4 to keep intact all notices c You must license the entire work as a whole under this License to anyone who comes into possession of a copy This License will therefore apply along with any applicable section 7 additional terms to the whole of the work and all its parts regardless of how they are packaged PN B04155AC D 5 Third Party Contributions and Legal Notices D 6 This License gives no permission to license the work in any other way but it does not invalidate such permission if you have separately received it d If the work has interactive user interfaces each must display Appropriate Legal Notices however if the Program has interactive interfaces that do not display Appropriate Legal
91. re 2 19 Transparency Button The resulting opaque help page eliminates the image of the screen behind it PN B04155AC 2 7 The Touch Screen Interface Help Messages 2 8 Figure 2 20 Home Page Help with opaque screen Home Page The Home Page is the first operational page to appear after the instrument startup You return to it when you touch the Home Page Button on the Header Bar It is the basic page from which you begin most operations In addition to the Header Bar and the Footer Bar the Home Page contains the following elements The wt Display appears when the wt mode is activa See the Set Speed Time Page for details The Set Speed Display Button takes you to the Set Speed Page The Set Time Display Button takes you to the Set Time Page The Set Temp Display Button takes you to the Set Temperature Page When you use Zonal Mode or Continuous Flow Mode the Zonal Continuous Flow Operation Page replaces the Home Page Table of Contents Select the Transparency Button again to return to the ghost screen image If you need information about some other aspect of the instrument you can select the Table of Contents link at the bottom of the page to see the outline of all the available help pages You have access to all of the help pages from the Table of Contents Figure 2 21 Help Navigation Buttons To navigate through your selected help pages use the Forward and Back buttons in the upper left corner PN B04155AC
92. re made available under the following license Microsoft Public License Ms PL This license governs use of the accompanying software If you use the software you accept this license If you do not accept the license do not use the software 1 Definitions The terms reproduce reproductions derivative works and distribution have the same meaning here as under U S copyright law A contribution is the original software or any additions or changes to the software A contributor is any person that distributes its contribution under this license D 1 Third Party Contributions and Legal Notices D 2 Licensed patents are a contributor s patent claims that read directly on its contribution 2 Grant of Rights a Copyright Grant Subject to the terms of this license including the license conditions and limitations in section 3 each contributor grants you a non exclusive worldwide royalty free copyright license to reproduce its contribution prepare derivative works of its contribution and distribute its contribution or any derivative works that you create b Patent Grant Subject to the terms of this license including the license conditions and limitations on patents to make have made use sell offer for sale import and or otherwise dispose of its contribution in the software or derivative works of the contribution in the software 3 Conditions and Limitations a No Tradem
93. reconditioning the chamber When the chamber reaches the set temperature you are ready to start the run Starting the Run To start the run press the Start Button and wait for the rotor to reach loading speed The status display at the top of the screen highlights Starting For a Continuous Flow run you need to perform alignment and adjustment checks as detailed in the rotor manual You may need to use the Slow to Zero RPM button to make adjustments and restart with the Start Button Refer to Figure 4 9 When the rotor passes the alignment and adjustment checks set the new loading speed if it is different from the adjustment speed and press the Start Button again Figure 3 9 Continuous Flow Page Press Start to go to Load speed When the rotor reaches the Loading speed the status display highlights Loading to show that you have moved into the next phase Loading the Sample In the Loading phase you inject the sample into the medium in the rotor Again the details depend on the rotor and the type of operation as detailed in the rotor manual When you have finished all the specified loading steps close the door and select the Loading Complete button to go to the next phase PN B04155AC 3 9 Operations Zonal and Continuous Flow Operation Running the Sample The instrument highlights Running on the Status Display accelerates to the set run speed and begins counting down the set run time The run phase ends in one of t
94. rror 1 Make sure the rotor is installed properly Imbalance 2 Verify the rotor load is within limits specified in the rotor manual 3 Ifthe problem persists call Beckman Coulter Field Service A800 Alert Door Latch Door latch will not engage 1 Make sure the door is closed before pressing Start 2 If the problem persists call Beckman Coulter Field Service A802 Alert Door Latch Door latch changed states Call Beckman Coulter Field Service D803 Door Latch Door latch will not disengage Call Beckman Coulter Field Service C 7 C Diagnostics Diagnostics User Messages Chart PN B04155AC APPENDIX D Third Party Contributions and Legal Notices _ PN B04155AC Software accompanying this ultracentrifuge contains Beckman Coulter Inc s works Beckman Works and modified or unmodified binary form of third party contributions Third Party Works Rights to only the Third Party Works are offered or granted to downstream software recipients and developers under their respective licenses as provided below The licenses provided below do not apply to any portion of Beckman Works All intellectual property rights in and to Beckman Works including but not limited to copyright in and to Beckman Works any derivative work based on part or all of Beckman Works and images photographs text and other information that may be incorporated in Beckman Works are owned an
95. rvice D112 USB Port USB import export error USB device may be full or removed too quickly Save again with another USB device D113 Touch Screen Not Detected Touch Screen USB cable not connected Be sure USB touch screen cable is connected If problem persists call Beckman Coulter Field Service D114 Code error Bad checksum on code flash Call Beckman Coulter Field Service D115 Code error Corrupted data flash Note This condition will result in a 200 minute delay before the diagnostic can be cleared and the door opened The power must be left on until the delay period is completed Call Beckman Coulter Field Service A117 Alert Printer error No printer or printer driver Be sure printer is turned on and drivers are installed If problem persists call Beckman Coulter Field Service A118 Alert Hard drive error Enhanced Write Filter disabled Call Beckman Coulter Field Service D119 SBC Communication Unable to open serial port Call Beckman Coulter Field Service A120 Alert Simulator Simulator not found Call Beckman Coulter Field Service C2 PN B04155AC PN B04155AC Table C 1 Diagnostics User Messages Chart Continued Diagnostics Diagnostics User Messages Chart Message Definition Result Recommended Action D121 Internal software error Software error Call Beckman Coulter Field Service
96. ry iii Safety and Notices v Alerts for Danger Warning Caution Important and Note v Safety Notices vi Information vi Installation vi Replacement Parts vii Service vii Power Supply vii Fuse Replacement vii Mechanical Safety viii Chemical and Biological Safety viii Precautions with Liquids ix Volatile Liquids ix Certification ix Scope of Manual ix CFC Free Centrifugation x Software Copyright x Biohazard x RoHS Notice xi China RoHS Caution Label xi China RoHS Environmental Label xi Summary of Instrument Labels xii Caution Symbol xii Universal Serial Bus USB xii Ethernet xii Biohazard xii Recycling Label xiii Multiple Compliance xiii CAUTION Static Sensitive Area xiv DANGER High Voltage xiv Protective Ground xiv Alternating Current xiv xvii Contents Output Plate Label xv Rotor Rotation xv Symbols xvi CHAPTER 1 Description 1 1 For In Vitro Diagnostic Use 1 1 Touch Screen 1 1 Name Rating Plate 1 1 Rotor Chamber 1 2 Vacuum System 1 2 Temperature Sensing and Control 1 2 Drive 1 2 Safety Features 1 3 Door 1 3 Barrier Ring 1 3 Imbalance Detector 1 3 Overspeed System 1 3 Dynamic Rotor Inertia Check DRIC 1 3 Specifications 1 4 Control Features 1 4 Physical Data 1 5 Audible Sounds 1 8 Available Rotors 1 8 CHAPTER 2 The Touch Screen Interface 2 1 Areas on the Screen 2 1 Header Bar 2 2 Home Page Button 2 2 Menu Button 2 2 Status Di
97. s Page 4 12 System Options Page 4 13 Zonal Authorization Page 4 14 Continuous Flow Authorization Page 4 15 Service Login Page 4 16 About Page 4 17 Rotor Catalog Page 4 18 Labware Catalog Page 4 19 Export User Manual Page 4 20 Set System Name Page 4 21 Set Date and Time Page 4 22 Set Sound Page 4 23 Select Language Page 4 24 Diagnostic History Page 4 25 Compatible Tubes for Rotor Page 4 26 Zonal Operation Page 4 27 Continuous Flow Operation Page 4 28 Running Home Page 4 29 Panel screws and Tabs 5 5 Internal Parts Configuration A vents through the port cap 5 6 Internal Parts Configuration B vents through the vacuum solenoid release screw 5 7 Safety and ventilation space A 2 Electrical Connection A 3 xxiii Illustrations xxiv Tables Tables Al Required Wire Connections A 4 cd Diagnostics User Messages Chart C 1 xxiv Tables XXV CHAPTER 1 Description _ The Optima XE generates centrifugal forces for the separation of particles Classified S it can be used with all currently manufactured Beckman Coulter preparative rotors This chapter describes the major components of the instrument For In Vitro Diagnostic Use This Optima XE centrifuge is intended for the separation of components through the use of relative centrifugal force It is designed to separate human samples including blood and other body fluids for processing analysis and
98. s the following elements e The List of Rotors on the left side of the screen shows all the available rotors for the instrument You can scroll through this list and select a rotor to view the detailed information about it e The Rotor Details on the right side of the screen shows all the details for the selected rotor on the List of Rotors The Back Button returns you to the References Page e The Labware Button takes you to the Compatible Tubes for Rotor Page to show you the labware for the selected rotor Note that this is a small subset of all the labware shown on the Labware Catalog Page PN B04155AC Functional Pages 4 Labware Catalog Page Labware Catalog Page Volume Type Part Number Volume Length Diameter 28 7 mL Konical 23 3 mL Konical 12 5 mL Konical Top Shape 8 5 mL Konical Bottom Shape Konical 8 5 mL Konical Polyallomer SW 28 SW 32 TI 42mL Konical 3 4 mt Konical Set Speed 15 000 RPM Set Time 005 hmm The Labware Catalog Page appears when you select the Labware Catalog Button on the References Page allowing you to examine the detailed specifications for the labware available for the rotors your instrument can use This is different from the Compatible Tubes for Rotor Page which shows only the labware available for a particular rotor when you are on the Rotor Catalog Page In addition to the Header Bar and the Footer Bar the Labware Catalog Page contains the following elements
99. s you to the System Options Page without changing the current volume and key click settings The OK button accepts your changes and returns you to the System Options Page PN B04155AC 4 23 Functional Pages Select Language Page Select Language Page Select Language Date and Time Format English United States English Zimbabwe Espa ol Argentina Espa ol Bolivia Espa ol Chile Espa ol Colombia Espa ol Costa Rica Espa ol Ecuador Set Speed 15 000 RPM The Select Language Page appears when you select the Select Language Button on the System Options Page allowing you to choose the language the instrument uses in operations In addition to the Header Bar and the Footer Bar the Select Language Page contains the following elements e The List of Languages shows all the languages and countries available for the instrument Scroll through this list and select a language and country The Cancel Button ignores your selection and returns you to the System Options Page e The OK Button accepts your selection and returns you to the System Options Page with the newly selected language active on all pages NOTE Be careful that you do not select a language that you do not understand You will find it difficult to return to this page and restore the language setting unless you can remember Menu upper left button for Options and then upper left button for Select Language 4 24 PN BO4155AC Functional Pages Diag
100. splay 2 3 Help Button 2 4 Footer Bar 2 4 Start Button 2 4 Stop Button 2 4 Footer Bar on the Home Page 2 5 Footer Bar on Other Pages 2 5 Vacuum Display Button 2 5 System Name 2 5 Accel and Decel Display Buttons 2 6 Page Display Area 2 6 Help Messages 2 6 Item Help 2 6 Global Help 2 7 xviii CHAPTER 3 CHAPTER 4 Contents Operations 3 1 Manual Operation 3 1 Step 1 Start on the Home Page 3 2 Step 2 Set the Speed and Rotor 3 3 Step 3 Set the Time 3 5 Step 4 Set the Temperature 3 6 Step 5 Start the Run 3 7 Zonal and Continuous Flow Operation 3 7 Preparing for the Run 3 8 Starting the Run 3 9 Loading the Sample 3 9 Running the Sample 3 10 Unloading the Sample 3 10 Stopping the Run 3 10 Finishing the Run 3 10 Functional Pages 4 1 Home Page 4 2 w t Display 4 2 Set Speed Display Button 4 3 Set Time Display Button 4 3 Set Temp Display Button 4 3 Menu Page 4 4 Set Speed Page 4 5 Set Time Page 4 6 Set Speed Time Page wt Mode 4 7 Set Temperature Page 4 8 Set Acceleration Deceleration Profiles Page 4 9 Example Acceleration and Deceleration Profiles 4 10 Select Rotor and Labware 4 11 References Page 4 12 System Options Page 4 13 Zonal Authorization Page 4 14 Continuous Flow Authorization Page 4 15 Service Login Page 4 16 About Page 4 17 Rotor Catalog Page 4 18 Labware Catalog Page 4 19 Export User Manual Page 4 20 Set System Name Page 4 21 xi
101. the Continuous Flow Operation Page which replaces the Home Page while you are using Continuous Flow mode PN B04155AC 4 15 4 Functional Pages Service Login Page Service Login Page Figure 4 18 Service Login Page Service Login 15 000 RPM Set n Set Temp 25 C The Service Login Page appears when you select the Service Mode Button on the Menu Page This page is for service personnel only If you accidently find yourself on the Service Login Page select the Cancel button to dismiss the page 4 16 PN B04155AC About Page Figure 4 19 About Page Optima XE 80 Ultracentrifuge Beckman Coulter Inc 2011 250 South Kraemer Boulevard Brea CA 92521 6232 Hotline 800 369 0333 Nitpiwww beckmancaultercom Ali nghts reserved This Computer program is protected by intemational copyright laws any unauthorized c use distribution transfer or sale is a violation of those T ed MOY fecal cA or CAMAT penlies This computer program ts also subject lo additional restrictions contained in a license granted by Beckman 4 Set Time 0 05 hmm Service Contact Service Phone Sales Contact Sales Phone System ID System Model Max Speed Pump Type Instrument S N Functional Pages About Page 1 800 369 0333 1 800 742 2345 Firmware Version IP Address 00 10 14136 141 The About Page appears when you select the About Button on the Menu Page This page presents system information about your instru
102. the three latch screws use the small flat head screwdriver to turn it clockwise until snug 6 After the panels are all secure reconnect the instrument to the power supply The instrument is again ready for use when power is restored PN B04155AC 5 9 Maintenance and Troubleshooting Storage and Transportation Storage and Transportation To ensure that the instrument does not get damaged contact Beckman Coulter Field Service for specific instructions and or assistance in preparing the equipment for transport or long term storage Supply List Contact Beckman Coulter Sales for assistance ordering parts and supplies Customers in the United States call 1 800 742 2345 For international contacts see the website at www beckmancoulter com or use the contact numbers on the inside front cover of this book A partial list of supplies is given below for your convenience See the Beckman Coulter Ultracentrifuge Rotors Tubes amp Accessories catalog BR 8101 available at www beckmancoulter com for detailed information on ordering rotors tubes and accessories Replacement Parts Description Part Number Chamber O ring 801778 Rotor Pad B42711 Supplies Description Part Number Silicone vacuum grease 2 02 335148 Beckman Solution 555 1 qt 339555 Logbook for preparative ultracentrifuges 330049 Master rotor logbook 339587 NOTE For MSDS information go to the Beckman Coulter website at w
103. tion 13 concerning interaction through a network will apply to the combination as such 14 Revised Versions of this License The Free Software Foundation may publish revised and or new versions of the GNU General Public License from time to time Such new versions will be similar in spirit to the present version but may differ in detail to address new problems or concerns Each version is given a distinguishing version number If the Program specifies that a certain numbered version of the GNU General Public License or any later version applies to it you have the option of following the terms and conditions either of that numbered version or of any later version published by the Free Software Foundation If the Program does not specify a version number of the GNU General Public License you may choose any version ever published by the Free Software Foundation PN B04155AC Third Party Contributions and Legal Notices D If the Program specifies that a proxy can decide which future versions of the GNU General Public License can be used that proxy s public statement of acceptance of a version permanently authorizes you to choose that version for the Program Later license versions may give you additional or different permissions However no additional obligations are imposed on any author or copyright holder as a result of your choosing to follow a later version 15 Disclaimer of Warranty THERE IS NO WARRANTY FOR THE PROGRAM TO T
104. tion Profiles Page Example Acceleration and Deceleration Profiles The values associated with each acceleration and deceleration profile are given in the tables below NOTE The values shown should be considered as approximations for time and rpm Acceleration Deceleration Profile Time RPM Profile Time RPM 0 Max 0 00 0 0 Max 0 00 0 1 2 00 170 1 2 00 170 2 2 40 350 2 2 40 350 3 3 00 500 3 3 00 500 4 3 00 170 4 3 00 170 5 4 00 350 5 4 00 350 6 4 30 500 6 4 30 500 7 4 00 170 7 4 00 170 8 5 20 350 8 5 20 350 9 6 00 500 9 6 00 500 10 Coast For example at an acceleration profile value of 5 the instrument takes four minutes to accelerate to 350 rpm then proceeds to the run speed at maximum acceleration For a deceleration profile of 3 the instrument uses maximum deceleration until it reaches 500 rpm then takes three minutes to slow to a stop The exception is a deceleration profile of 10 for which no braking is applied at all This is generally not used for high speed runs because of the extremely long length of time it would take for the rotor to come to a stop 4 10 PN BO4155AC Functional Pages 4 Select Rotor and Labware Select Rotor and Labware Figure 4 13 Select Rotor and Labware Page Select Rotor and Labware No Rotor las Volume Type P N Type 100 Ti B No Tube Type 90 Ti Type 70 Ti Type 70 1 Ti Type 50 4 Ti Type 50 271 Gancel
105. ult Call Beckman Coulter Field Service D601 Drive Bus current too high gt 30A Call Beckman Coulter Field Service D602 Drive Drive temperature gt 69 C Call Beckman Coulter Field Service Temperature D603 Damper Damper coil status error Call Beckman Coulter Field Service D604 CF 32 Rotor Oil CF 32 oil level error Make sure the rotor oil level switch is Level installed on the back panel for CF 32 operation Add oil to CF 32 rotor D605 Drive Abnormal rate of change in Call Beckman Coulter Field Service Performance speed PN B04155AC PN B04155AC Table C 1 Diagnostics User Messages Chart Continued Diagnostics Diagnostics User Messages Chart Message Definition Result Recommended Action D606 Drive Abnormal tachometer NOTE This condition will result in a 200 Performance frequency minute delay before the diagnostic can be cleared and the door opened The power must be left on until the delay period is completed 1 Make sure the rotor is installed properly 2 Check the condition of the rotor overspeed disk 3 Verify the rotor load is within limits specified in the rotor manual 4 Ifthe problem persists call Beckman Coulter Field Service D607 Drive Drive frequency error Call Beckman Coulter Field Service Performance D608 Drive Tachometer frequency gt 103K Call Beckman Coulter Field Service Performance rpm A700 Alert Imbalance detector e
106. uring the run causing automatic shutdown if rotor loads are severely out of balance Even at low speeds an incorrectly loaded rotor can cause an imbalance Overspeed System The overspeed system is a safety feature designed to ensure that the rotor does not exceed its maximum allowable speed This system includes a photoelectric device in the rotor chamber next to the drive spindle and an overspeed disk on the bottom of the rotor Individual rotor manuals provide information on the correct overspeed disks to be used with each rotor The overspeed disk has alternating light and dark sectors As the rotor spins the passage of reflecting and non reflecting sectors over the photoelectric device generates a series of pulses detected by the electronic circuitry and software After the rotor reaches 600 rpm the set speed is checked against the overspeed disk If the set speed is greater than the maximum speed permitted by the disk the speed setting is automatically lowered to the maximum speed of the disk but the run continues without interruption An error message appears to alert you to the change Dynamic Rotor Inertia Check DRIC PN B04155AC As the rotor accelerates between 15000 and 20000 rpm the instrument checks the rotor inertia and calculates the rotor energy for the speed set by the user If the calculated rotor energy is excessive the instrument stops the run with braking to avoid possible damage and sends a diagnostic message to
107. ust require no special password or key for unpacking reading or copying 7 Additional Terms Additional permissions are terms that supplement the terms of this license by making exceptions from one or more of its conditions Additional permissions that are applicable to the entire Program shall be treated as though they were included in this License to the extent that they are valid under applicable law If additional permissions apply only to part of the Program that part may be used separately under those permissions but the entire Program remains governed by this License without regard to the additional permissions When you convey a copy of a covered work you may at your option remove any additional permissions from that copy or from any part of it Additional permissions may be written to require their own removal in certain cases when you modify the work You may place additional permissions on material added by you to a covered work for which you have or can give appropriate copyright permission PN BO4155AC D 7 Third Party Contributions and Legal Notices D 8 Notwithstanding any other provisions of this License for material you add to a covered work you may if authorized by the copyright holders of that material supplement the terms of this License with terms a Disclaiming warranty or limiting liability differently from the terms of sections 15 and 16 of this License or b Requiring preservation of
108. vered work in accord with this section you must place in the relevant source files a statement of the additional terms that apply to those files or a notice indicating where to find the applicable terms Additional terms permissive or non permissive may be stated in the form of a separately written license or stated as exceptions the above requirements apply either way 8 Termination You may not propagate or modify a covered work except as expressly provided under this License any attempt otherwise to propagate or modify it is void and will automatically terminate your rights under this License including any patent licenses granted under the third paragraph of section 11 However if you cease all violation of this License then your license from a particular copyright holder is reinstated a provisionally unless and until the copyright holder explicitly and finally terminates your license and b permanently if the copyright holder fails to notify you of the violation by some reasonable means prior to 60 days after the cessation Moreover your license from a particular copyright holder is reinstated permanently if the copyright holder notifies you of the violation by some reasonable means this is the first time you have received notice of violation of this License for any work from that copyright holder and you cure the violation prior to 30 days after your receipt of the notice Termination of your rights under this section d
109. vice D402 Slow Vacuum Vacuum not lt 20 microns after 20 minutes 1 Make sure door o ring is clean undamaged and properly lubricated 2 Check for sample leakage Clean and dry the rotor chamber if needed 3 If the problem persists call Beckman Coulter Field Service D403 Lost Vacuum Vacuum gt 750 microns for 1 minute after being lt 750 microns 1 Make sure door o ring is clean undamaged and properly lubricated 2 Check for sample leakage Clean and dry the rotor chamber if needed 3 If the problem persists call Beckman Coulter Field Service D404 Lost Vacuum Vacuum gt 50 microns for 10 minutes after being lt 20 microns 1 Make sure door o ring is clean undamaged and properly lubricated 2 Check for sample leakage Clean and dry the rotor chamber if needed 3 If the problem persists call Beckman Coulter Field Service D407 Vacuum Vent Vacuum vent solenoid not connected Call Beckman Coulter Field Service D408 Vacuum Vent Vacuum vent can not be opened Call Beckman Coulter Field Service D500 Temperature Control Ambient thermistor open Call Beckman Coulter Field Service D501 Temperature Control Ambient thermistor shorted Call Beckman Coulter Field Service D502 Temperature Control Ambient temperature out of range lt 10 C or gt 35 C Adjust room temperature before operating D503 Temperature Control
110. w operations have been authorized the Zonal Operation Page or the Continuous Flow Operation Page replaces the Home Page The mode does not end until the run is complete or the user selects the Cancel Button to end the mode Zonal and Continuous Flow operations are very similar The specific details vary according to the rotor you are using but the summary below shows an outline of the procedure Press Start to go to Load speed Loading Complete Unload Load Speed Vacuum Unload Speed ay There are seven phases to a Zonal or Continuous Flow run Preparing Starting Loading e Running Unloading Stopping e Finishing Each phase is outlined in a section below Preparing for the Run 3 8 The rotor manual details the preparations for a run which may include cleaning assembling and lubricating the rotor setting up pumps and tubing and usually includes overnight cooling to precondition the rotor sample and solutions used in the run When you are ready to install the rotor set the parameters for the run Run Speed e Run Time e Temperature Load Speed e Unload Speed PN B04155AC Operations 3 Zonal and Continuous Flow Operation For Continuous Flow operation you may have two different load speed settings The initial setting is for alignment and adjustment checks which you set in this phase When everything else is ready mount the rotor and select the Vacuum Display Button to begin p
111. ww beckmancoulter com 5 10 PN B04155AC APPENDIX A Preinstallation Requirements Overview NOTE Do not attempt to install or turn on the power to the Optima XE Its purchase price includes installation by Beckman Coulter personnel Installation by anyone other than authorized Beckman Coulter personnel invalidates the instrument warranty Preinstallation requirements have been sent prior to shipment of the instrument Copies are also attached to the outside of the shipping container The following information is provided in case the instrument must be relocated Contact Beckman Coulter Field Service to adjust and level the instrument if it must be moved The pads on each leveling leg are designed to prevent possible rotation of the instrument in the case of a rotor mishap Space Requirements Space requirements include specifications for safety ventilation and temperature Safety IMPORTANT This unit or system is provided with fixed trip limits and shall not be aggregated above 30kW on a single point of common connection IMPORTANT To reduce the risk of fire connect only to a circuit provided with 30 amperes maximum branch circuit overcurrent protection in accordance with the National Electrical Code ANSI NFPA 70 IMPORTANT Additionally Equipment shall be installed on a dedicated branch circuit e The branch circuit protection shall be one that is suitable to be back fed e Circuit breakers that are marked with a
112. x Contents XX CHAPTER 5 APPENDIX A APPENDIX B Set Date and Time Page 4 22 Set Sound Page 4 23 Select Language Page 4 24 Diagnostic History Page 4 25 Compatible Tubes for Rotor Page 4 26 Zonal Operation Page 4 27 Continuous Flow Operation Page 4 28 Running Home Page 4 29 Maintenance and Troubleshooting 5 1 Field Service 5 1 Rotors and Labware 5 1 Cleaning 5 1 Instrument Surfaces 5 2 Rotor Chamber 5 2 Chamber Door O ring 5 2 Decontamination 5 2 Sterilization and Disinfection 5 3 Diagnostics User Messages 5 3 Retrieving Your Sample in Case of Power Failure 5 3 During a Run 5 3 Rotor Spinning at Restoration 5 3 Rotor Stopped at Restoration 5 4 Getting Access to the Sample 5 4 Restoring the Instrument to Operating Condition 5 9 Storage and Transportation 5 10 Supply List 5 10 Replacement Parts 5 10 Supplies 5 10 Preinstallation Requirements A 1 Overview A 1 Space Requirements A 1 Safety A 1 Ventilation A 2 Temperature A 2 Electrical Requirements A 3 Special Warranty for the Optima XE B 1 Special Warranty B 1 APPENDIX C APPENDIX D Contents Diagnostics C 1 Overview C 1 Diagnostics User Messages Chart C 1 Third Party Contributions and Legal Notices D 1 xxi Illustrations Illustrations 2 1 Home Page 2 1 22 Header Bar 2 2 23 Home Page Button 2 2 2 4 Menu Button 2 2 2 5 Ready Status 2 3 2 6 Header Bar Ready Status 2 3 2 7 Head

Optima XE Series User Guide

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