Optima L Series
Contents
1. Key Switch OPERATIONAL FEATURES Revolution Counter Barrier Ring Introduction Indicates rotor speed in increments of 10 rpm at speeds below 1000 rpm and increments of 100 rpm at speed above 1000 rpm Up to 99 hours and 59 minutes HOLD for runs of unspecified length Indicates time remaining in timed runs time elapsed in HOLD runs and estimated time remaining in f runs 0 to 40 C in increments of 1 C 0 5 C of set temperature Indicates rotor temperature in increments of 0 1 C Within 0 3 C of set after equilibration 15 to 40 C Up to 9 99 x 1014 radians squared per second Shows accumulated centrifugal force to three significant digits in exponential notation Selection from two profiles maximum acceleration from 0 rpm to set speed or slow acceleration from 0 to 500 rpm followed by maximum acceleration to set speed Selection from three profiles full dynamic braking to 0 rpm slow full brake to 500 rpm followed by reduced braking to a gentle stop or no brake Standard programmed or delayed start instrument memory can store up to nine programs Used to select normal or zonal operation Displays accumulated total number of rotor revolutions in tens of thousands 41 mm 1 63 in heat treated steel alloy armor ring surrounded by a 12 mm 0 50 in steel vacuum chamber to provide full protection for the operator 17 5 mm 0 69 in high streng2. 0 0000 e eee 4 3 Figure 4 2 Depressing the Latch to Loosen the Front Panel 4 4 Figure 4 3 Interior View of Instrument 0 0 0 0c c eee eee eee 4 5 Figure 4 4 Location of Diagnostic Messages on the Control Panel 4 6 Tables Page Table 1 1 Run Status Indicators llle eee 1 4 Table 2 1 Vacuum LEEDS entr emoten tok Ere Re ORO E e 2 9 Table 4 1 Diagnostic Troubleshooting Chart 0 0 0 0 ee eee eee 4 7 Introduction INSTRUMENT CERTIFICATION SCOPE OF MANUAL To assure full system quality the Beckman Coulter Optima L series ultracentrifuges have been manufactured in a registered ISO 9001 facility They have been designed and tested to be compliant when used with Beckman Coulter rotors with the laboratory equipment requirements of applicable regulatory agencies Declarations of conformity and certificates of compliance are available at www beckmancoulter com This manual is designed to familiarize you with the Beckman Coulter Optima L 100K or L 90K Preparative Ultracentrifuge its function specifications operation and routine operator care and maintenance The following introductory pages contain the instrument specifica tions and preinstallation requirements Section 1 provides a brief description of the instrument the operating controls and indicators Section 2 explains how to use the control panel to enter run parameters for standard and pr
3. Press and START The rotor accelerates to the loading speed either the default speed of 2000 rpm or a user specified speed between 2000 and 3000 rpm The rotor remains spinning at the loading speed until the chamber door is closed ACCELERATION DECELERATION Run Procedure III NOTE If you are performing consecutive zonal runs at the end of each zonal run the centrifuge will automatically reset to the normal mode To reconfirm selection of the zonal mode you must turn the key left to right clockwise from the zonal position to the normal position and then back to the zonal position Then press and again to begin the run If you press and without having reset the key the DOOR diag nostic message will be displayed and the instru ment will not start Press to clear the diagnostic to begin operation turn the key as described above and press and again 6 Load the rotor 7 Disconnect the seal assembly and cap the rotor 8 Close the chamber door The vacuum system will activate auto matically The rotor will hold at 3000 rpm until the chamber pressure drops below 750 microns at which time it will accelerate to set speed and the instrument will begin counting time remaining or time lapsed 9 When the run is ended time remaining at zero w2t value reached Or pressed the rotor decelerates to 2000 rpm gt NOTE To abort a zonal run in progress press STOP When the rotor speed decelerates to 2000
4. Earth Y Ground 30 ampere Circuit P x e Required Wire Connections Earth ground Neutral Live or Line To ensure safety the instrument should be wired to a remote emer gency switch preferably outside the room where the centrifuge is housed or adjacent to the exit from that room in order to disconnect the instrument from the main power source in case of a malfunction Locate the Optima L in a clean safe uncluttered environment Be sure to provide a 5 1 cm 2 in clearance on each side as the feet extend about 5 1 cm 2 in beyond the instrument see the Figure below The pads under each foot have been designed to prevent possible rotation of the instrument in the event of a rotor mishap A 15 2 cm 6 in clearance is required at the rear of the instrument for servicing the control panel and to ensure sufficient air ventilation during operation d Ln Outlet Hubbell 9330 Bryant 96 30 FR or Equivalent NEMA 6 30 R Introduction The instrument will operate within specifications in a laboratory with ambient temperatures ranging from 15 to 40 C N WARNING Do not place the instrument near areas containing flammable reagents or combustible fluids SJ 0 c E 5 gt 6 i Mu 26 5 A Nw N oe 7T XU Bo 51 2 cm XV POWER STANDBY MODE 1 Description The Optima L 100K or L 90K Preparative Ultracentrifuge classi
5. Coulter Field Service for assistance Troubleshooting and Maintenance Table 4 1 Diagnostic Troubleshooting Chart SPEED Speed has been set above the Check set speed check the rotor for clean undam maximum allowable for the rotor or aged and correct overspeed disk see the Rotors wrong damaged or missing overspeed and Tubes manual LR IM for replacement disk on the installed rotor instructions TEMP Temperature control or vacuum system Check the air inlet at bottom of the front panel for malfunctioning obstructions Call Beckman Coulter Field Service if problem persists DRIVE Abnormal change in drive speed or Be sure a rotor is properly installed on the spindle if overheated drive power has failed wait for 5 minutes for drive to cool check for air inlet obstruction VAC Vacuum not being drawn properly e Check door O ring for damage and dirt e Check rotor lid O rings for possible leakage e Check the vacuum oil If milky in color run the vacuum system for several hours or overnight until the oil is clear IMBAL Rotor imbalance at low speeds Check for proper rotor loading DOOR Door is open when the key is Be sure door is closed pressed PWR Loss of power during run Check TIME display run may need to be restarted or aborted CPU Microprocessor malfunction or loss of No user action Call Beckman Coulter Field Service program memory CLEANING gt NOTE Before using any cleaning
6. The display continues to blink until you press another parameter key Or ENTER RECALL If you press another parameter key the associated display begins to blink and the set values continue to be displayed If you press ENTER RECALL the instrument displays the set values for 5 seconds then exits the editing mode and returns to showing actual conditions Set values remain in memory until new values are entered Whenever you press ENTER RECALL the instrument displays the set values for 5 seconds then returns to showing actual conditions 1 3 Description 1 4 RUN STATUS INDICATORS Run indicators are provided in the form of green and red LEDs to indicate the status of the instrument Figure 1 1 shows the location of the LEDs on the control panel and Table 1 1 describes the meaning of each Unless otherwise noted the LEDs appear above their respec tive keys Table 1 1 Run Status Indicators START A green LED on the key lights when the key is pressed It blinks until the rotor reaches set speed and then shines continuously until the run ends or is pressed STOP A green LED on the key lights when either the key is pressed or the rotor begins to decelerate It blinks until the rotor comes to a stop ACCEL MAX or SLOW indicates which acceleration profile has been selected DECEL MAX SLOW or NO BRAKE indicates which deceleration profile has been selected at a t indicates that the ultrace
7. The rotor will accelerate or decelerate to the new speed The rotor will not accelerate beyond 3000 rpm until the chamber pressure drops below 750 microns At that point the rotor acceler ates to set speed e If any speed related malfunction occurs a SPEED diagnostic message will appear Consult the Diagnostic Troubleshooting Chart in Section 4 for details A special delayed start program function automatically sets the speed at 0 rpm This function is described at the end of this section How the run is terminated depends upon the time mode selected TIME w2t or HOLD The information that appears in the TIME HR MIN display also depends upon the mode selected The TIME mode is discussed here a description of HOLD and o t modes follow The run time can be set up to 99 hours and 59 minutes During the run the display indicates the time remaining in hours and minutes To enter or modify the set time 1 Press TIME The value in the TIME display blinks 2 Use the keypad to enter the run time in hours and minutes 3 Press another parameter key or to save the run time If you entered a number that is between 60 and 99 minutes the instru ment automatically recalculates the time in hours and minutes Accumulated Centrifugal Effect Run Preparation When and have been pressed the instrument checks to make sure that a value has been entered in the TIME display that a value for w2t has been entered in the speed
8. display or that the HOLD mode has been selected In the TIME mode the TIME display begins counting down as the rotor begins to spin When the TIME display reaches zero the run ends and the rotor automatically decelerates to a stop The 1 display indicates the accumulated centrifugal effect in radians squared per second to three significant figures using expo nential notation The w2t can be set for up to 9 99 x 1014 Decelera tion begins when the set t value is reached The w2r value is shown in the SPEED display when the instrument is in the 2t mode and the key is pressed To enter or modify the set 2 value 1 Press or The value in the SPEED display blinks 2 Use the keypad to enter an 9 t value An E remains stationary in the hundreds place to indicate that the last two places represent the exponent If you enter an invalid number such as 0E22 the instrument will beep twice Press to clear the value then enter a valid number 3 Press another parameter key or to save the 21 value The t indicator will light Example To enter 3 24 x 1012 as the wt value Press wr Press 3 O 2 O in that order The display will show 3 24E12 Press another parameter key or ENTER RECALL In the 2t mode a calculation of the time remaining until the set t value is reached is shown in the TIME display If the calculation exceeds 99 hours and 59 minutes four dashes will appear in the TIM
9. instrument and damage electrical or mechanical components Safety Against Risk of Fire Fuses protect certain electrical circuits within this instrument against overcurrent condi tions For continued protection against the risk of fire replace only with the same type and rating specified This instrument is not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials for example chloroform or ethyl alcohol in this instrument nor handle or store them near the centrifuge Mechanical Safety For safe operation of the equipment observe the following e Use only the Beckman Coulter rotors and accessories designed for use in this instrument Do not exceed the maximum rated speed of the rotor in use NEVER attempt to slow or stop a rotor by hand Do not move the centrifuge while the drive motor is spinning nthe event of a power failure do not attempt to retrieve the sample from the instru ment for at least one hour Then follow the instructions for recovery of the sample under TROUBLESHOOTING AND MAINTENANCE Chemical and Biological Safety Normal operation may involve the use of solutions and test samples that are pathogenic toxic or radioactive Such materials should not be used in this instrument however unless all necessary safety precautions are taken Handle body fluids with care because they can transmit disease No known test offers complete assurance
10. no need to reenter run conditions unless you wish to make a change PROGRAMMED OPERATION 1 With the power on open the chamber door install the rotor and close the chamber door Press if you want to evacuate the chamber now 2 Press and use the keypad to select a program number Or press repeatedly until the required program appears Run Procedure 3 Press and START to begin the run The vacuum system is automatically activated now unless you pressed earlier Any of the run conditions can be changed while a programmed run is in progress Such changes will affect only the current run To change a parameter during the run 1 Press a parameter key for example 2 Enter the new value 3 Press ENTER RECALL The run in progress will be modified accordingly Runs in the timed and t modes will terminate automatically when the set value is reached 4 After the rotor has stopped press to vent the chamber 5 Open the chamber door remove the rotor and close the door To keep the rotor chamber clean and dry keep the door closed between runs DELAYED START PROGRAM FUNCTION 1 With the power on open the chamber door install the rotor and close the chamber door 2 Press PROG Use the keypad to press 0 and then the number for the second program Press ENTER RECALL 3 Press and START The word delay will appear in the SPEED display and the second program number you selected will ap
11. or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment 4 7 Troublehshooting and Maintenance 4 8 DECONTAMINATION Keep instrument surfaces clean by wiping them with a cloth damp ened with a mild detergent such as Beckman Solution 555 diluted 5 or 10 to 1 with water If salts or other corrosive materials are used however or if spillage occurs wash all affected areas immediately Do not allow corrosive materials to dry on the instrument Be careful not to spill liquid on the instrument where electrical or mechanical components could get damaged Keep the chamber door closed between runs to prevent moisture and dirt from collecting inside Wipe off the chamber walls with a dry cloth before each run If the instrument and or accessories are contaminated with radioac tive or pathogenic solutions follow appropriate decontamination procedures as determined by your laboratory safety officer Refer to Chemical Resistances publication IN 175 or contact Beckman Coulter Field Service to ensure that the decontamination method does not damage any part of the instrument or accessories STERILIZATION AND DISINFECTION The top working surface is finished with urethane paint the sides are finished with general purpose paint Ethanol 70 may be used on both these surfaces See publication IN 175 for more informati
12. that is the values that existed when the rotor began decelerating Follow the instructions below for using the parameter keys and keypad to enter run settings The run speed can be set between 1000 rpm and 100 000 rpm L 100K or 90 000 rpm L 90K in increments of 100 rpm When entering the set speed the last two digits in the SPEED RPM display remain fixed as zeros and cannot be changed Speeds between 0 and 1000 rpm are shown in increments of 10 for example 980 rpm Speeds above 1000 rpm are shown in increments of 100 for example 45 600 rpm To enter or modify the set speed 1 2 Press SPEED The value in the SPEED display blinks Use the keypad to enter or modify the speed If you try to enter an invalid number the instrument will beep twice Press to clear the value then enter a valid speed Run Preparation 3 Press another parameter key or to save the set speed After and have been pressed the set speed is checked against the overspeed disk at 1000 rpm If the speed setting is greater than that allowed by the overspeed disk the run will continue but the rotor speed will be adjusted to the maximum speed permitted by the disk The TIME setting is also changed to provide an equivalent t value if in the 2t mode A SPEED diagnostic message will appear indicating that these changes have been made Press to clear the message You may change the set speed at any time by repeating the steps listed above
13. that they are free of micro organisms Some of the most virulent Hepatitis B and C and HIV I V viruses atypical mycobacterium and certain systemic fungi further emphasize the need for aerosol protection Handle other infec tious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in a rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Labora tory Biosafety Manual are handled materials of a higher group require more than one level of protection Dispose of all waste solutions according to appropriate environmental health and safety guidelines It is your responsibility to decontaminate the instrument and accessories before requesting service by Beckman Coulter Field Service LL IM 9 September 2005 Deccan COULTER Optima L Series Preparative Ultracentrifuges Instruction Manual 2005 Beckman Coulter Inc Printed in the U S A CE Contents INTRODUCTION SECTION 1 Page Instrument Certification 0 060 eens vil Scope of Mandalas SR eas Lee WIRE Ra vii CONVENTIONS ix cs Sade Kee eds Koes EC eA aye RESERVE S viii Radio Inteterference csse ix SPECHICAHONS
14. the bottom of the rotor chamber see Figure 1 2 when the chamber pressure is below 100 microns When above 100 microns or if the radiometer fails chamber temperature is measured by a thermistor mounted in the chamber TEMPERATURE CONTROL SYSTEM The instrument uses a solid state thermoelectric refrigeration and heating system which eliminates the need for a complex conven tional refrigeration system and heater Neither Freon nor water is needed the only coolant required is forced air from the cooling fans After the power is turned on the temperature control system is acti vated when the door is closed and the vacuum system is turned on The rotor temperature is controlled to 0 5 C of the set value If the temperature system malfunctions the TEMP diagnostic message will appear and the rotor will decelerate to a stop Trademark of E I Du Pont de Nemours amp Company OVERSPEED SYSTEM Description The overspeed system is a safety feature designed to ensure that the rotor does not exceed its maximum allowable speed This system includes photoelectric sensors in the rotor chamber next to the drive spindle see Figure 1 2 and an overspeed disk on the bottom of the rotor Individual rotor manuals provide information on the correct overspeed disks to be used with each rotor The overspeed disk has alternating light and dark sectors As the rotor spins the passage of reflecting and nonreflecting sectors over the photoelect
15. 49 Master rotor logbook 0 0 0 cee eee ee 339587 Direct drive vacuum pump oil eee 341661 Diffusion pump Oil 6 ee eee 330246 4 9 SPECIAL WARRANTY FOR THE OPTIMA L LE L XP AND XL PREPARATIVE ULTRACENTRIFUGES Subject to the exceptions and upon the conditions specified below Beckman Coulter Inc agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within one 1 year after delivery of the Optima Ultracentrifuge the product to the original Buyer by Beckman Coulter or by an authorized representative provided that investigation and factory inspec tion by Beckman Coulter discloses that such defect developed under normal and proper use Some components and accessories by their nature are not intended to and will not function for as long as one 1 year If any such component or accessory fails to give reasonable service for a reasonable period of time Beckman Coulter will repair or at its election replace such component or accessory What constitutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter REPLACEMENT Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer with the trans portation charges collect unless the product is found to be defective in which case Beckman Coul
16. 564 85 901 SWITZERLAND Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 0800 850 810 Fax 0848 850 810 TAIWAN Beckman Coulter Taiwan Inc Taiwan Branch 8th Floor 216 Tun Hwa South Road Section 2 Taipei 106 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 TURKEY Beckman Coulter Ltd E 5 Yanyol Faith Cad 81410 Soganlik Kartal Istanbul Turkey Telephone 90 216 309 1900 Fax 90 216 309 0090 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court Kingsmead Business Park London Road High Wycombe Buckinghamshire HP11 1JU England U K Telephone 01494 441181 Fax 01494 447558 e mail beckman uk beckman com Authorized dealers in other countries 4300 N Harbor Boulevard Box 3100 Fullerton California 92834 3100 Internet www beckmancoulter com Telex 678413 Fax 1 800 643 4366 Cry QO Printed on recycled paper
17. E display After the run pressing ENTER RECALL STOP and in succession will show the accumulated w2t value in the SPEED display when the rotor began to decelerate After 5 seconds the display returns to showing actual conditions Run Preparation 2 6 Temperature The tf indicator will remain on until a different run mode is selected as a reminder that the instrument is still in the 2t mode The accu mulated w2t from the previous run will automatically return to zero when and START are pressed to begin a new run The HOLD mode is used for runs of unspecified lengths or runs that will last longer than 99 hours and 59 minutes When is pressed while the instrument is in this mode the word HOLD appears in the TIME display for 5 seconds The display will then return to showing the time elapsed since the beginning of the run To select the HOLD mode 1 Press HOLD The indicator lights to indicate that the instrument is in this mode The word HOLD appears in the TIME display 2 Press the next parameter key or ENTER RECALL After and have been pressed the run will continue indefinitely until you press STOP If the run exceeds 99 hours and 59 minutes dashes will appear in the TIME display The temperature can be set between 0 and 40 C in increments of 1 C If no other value has been entered the instrument automatically selects 25 C as the default operating temperature To enter or modify the set temperatu
18. LATION REQUIREMENTS ELECTRICAL DATA A CAUTION Do not attempt to install or turn on the power to the instrument Its purchase price includes installation by Beckman Coulter personnel Installation by anyone other than authorized Beckman Coulter personnel invalidates the warranty Preinstallation requirements have been sent prior to shipment of the instrument Copies are also attached to the outside of the shipping container The following information is provided in case the ultra centrifuge must be relocated Contact your Beckman Coulter Field Service representative to adjust and level the instrument if it must be moved Instrument rating 220 to 240 VAC 50 Hz 20 A 200 to 240 VAC 50 60 Hz 20 A Power line range 180 to 264 VAC 60 or 50 Hz single phase 30 A Electrical supply Class I To reduce the risk of electrical shock this equipment uses a three wire electrical cord 1 8 m 6 ft and plug to connect the equipment to earthground In regions where the instrument is supplied with an unterminated cord a plug that meets local electrical and safety requirements must be supplied Contact your local Beckman Coulter office for specific information regarding these requirements See the Table below for the required wire connections Make sure that the matching wall outlet receptacle is properly wired and earth grounded xiii Introduction xiv SPACE 30 d Circuit Protector Green Yellow Light Blue Brown
19. LL IM 9 SY BECKMAN COULTER Optima L Series Preparative Ultracentrifuges Instruction Manual Symbol Symbol Symbole Simbolo Simbolo Title Titel Titre Titulo Titolo X X Dangerous voltage Gefahrliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo alta tensione EREE Attention consult accompanying documents Achtung Begleitpapiere beachten Attention consulter les documents joints Atenci n consulte los documentos adjuntos Attenzione consultare le informazioni allegate UE GANDAHESRNOCC On power Ein Netzverbindung Marche mise sous tension Encendido Acceso sotto tensione A Bik Off power Aus Netzverbindung Arr t mise hors tension Apagado Spento fuori tensione Y GER Protective earth ground SchutzleiteranschluB Liaison a la terre Puesta a tierra de protecci n Collegamento di protezione a terra RETA 5H Earth ground Erde Terre Tierra Scarica a terra PX BM SAFETY NOTICE This safety notice summarizes information basic to the safe operation of the equipment described in this manual The international symbol displayed above is a reminder that all safety instructions should be read and understood before installation operation mainte nance or repair of this instrument When you see the symbol on other pages pay special attention to the safety information presente
20. OT be removed during a zonal operation 2 1 Run Preparation I gt NOTE As a Safety feature after you press and START you must reset the key by turning it out of the zonal position then back into the zonal position Then press and START again to begin the zonal run see Section 3 Figure 2 1 Inserting the Key Into the Interlock Switch Turn the key to the right to select normal operation and to the left to select zonal operation STANDARD OPERATION KEYPAD Use the keypad to make numerical entries As you enter each digit the digits already entered move one place to the left in the display Use the and keys as follows The key can clear a value after a parameter key has been pressed Clear Entry if the instrument is in the editing mode or it can clear a diagnostic message 2 2 ENTER RECALL PARAMETER KEYS Run Preparation Press once to clear a numerical entry Press twice to exit from the editing mode The display will stop blinking and the actual values will reappear Press once to clear a diagnostic message see Section 4 for more information Use the key to enter or display values as follows Press to enter a set value into memory Press at any time to display the settings for 5 seconds After 5 seconds the displays show the actual conditions again Press and then START within 5 seconds to start a run Press and then within 5 seconds to display the STOP values
21. Program 0 s seise use os me eae Rd 2 10 Running Program 0 with a Second Program 2 10 Editing Settings During the Delayed Start Run 2 11 RUN PROCEDURE Standard Operation soos Ea eee sede Sate ees 3 2 Repeating a Run 2 y teu ee a RR d eG 3 2 Programmed Operation 0 0 0 cece eee eee eee 3 2 Delayed Start Program Function 0 0 00 c eee eee eee 3 3 Zonal OperatioB i22 ous sais had sape dor op ta Goa e dr ae Ear te SUR 3 4 Accelefatloti asista tad ead acia nee els Ree 3 5 Deceleration oer Rm REIR eR n ERR e re 3 5 Unloading soi gee be vat nr RR ERES a E 3 6 Points to Remember 0 0 0 0 0 3 6 TROUBLESHOOTING AND MAINTENANCE In Case of Power Failure During the Run 4 1 Retrieving Your Sample 1 0 0 0 cee ee eee 4 2 Diagnostic Messages i csc stew Gas skate yeaa em ede Pew ee 4 6 Cleaning ssi ou wae hah sat Saal dao e por adie a a aaa a ee 4 7 Decontamination 00 cece eee eee 4 8 Sterilization and Disinfection 0 0 0 00 02 ee eee eee 4 8 Storage and Transportation 0 0 0c eee eee ee eee 4 8 Supply List i cesi e RE REDE FERRE IAN hE oes 4 9 Illustrations Page Figure 1 1 The Control Panel 2 0 eee eee 1 2 Figure 1 2 The Rotor Chamber 0 0 0 0 cece cece ee eee 1 5 Figure 2 1 Inserting the Key Into the Interlock Switch 2 2 Figure 4 1 Removing the Control Head Cover
22. SETTINGS DURING THE DELAYED START RUN I gt NOTE If you change a run setting during program 0 the second program is automatically canceled because the instrument is no longer in the program mode The instrument will stop at the end of program 0 To edit a setting during the run without permanently changing the program use the parameter keys and keypad as described above Then press ENTER RECALL Do NOT press SAVE The instrument will run using the modified settings The lighted number above the key will go out to indicate that the instrument is no longer running from program memory 2 11 3 Run Procedure This section summarizes standard programmed and zonal operation as well as the delayed start function Use any of the acceptable Beckman Coulter rotors consulting the applicable rotor manual and the Rotors and Tubes Manual LR IM for instructions on preparing the rotor and accessories for centrifugation For fast temperature equilibration refrigerate or warm the rotor to the required temperature before the run A WARNING Do not use the ultracentrifuge in the vicinity of flammable liquids or vapors and do not run such materials in the instrument Do not lean on the instrument or place items on it while it is operating A WARNING Normal operation may involve the use of solu tions and test samples that are pathogenic toxic or radioactive Operator error or tube failure may generate aerosols D
23. ake sure the chamber door is closed then press VACUUM The chamber door can be opened only when the vacuum has been completely vented and the rotor is at rest When the run begins the rotor will not accelerate past 3000 rpm until the chamber pressure drops below 750 microns At that time the rotor accelerates to set speed As the vacuum system begins to evacuate the chamber a series of three LEDs labeled 750 200 and 20 microns blink remain lighted or go off to allow you to monitor the change in pressure see Table 2 1 When the pressure drops below 20 microns the 20 LED will remain lit until is pressed to vent the chamber at the end of the run Press and to begin a run If you press only START the instrument will not be activated If this occurs press and START again to begin the run Run Preparation Table 2 1 Vacuum LEDs 750 or above blinking off off 220 to 750 lit blinking off 20 to 200 off lit blinking lt 20 off off lit After you have pressed and START the instrument checks to see if there is an entry in the TIME display or if 2r or HOLD has been selected If there is no entry the instrument will beep twice and the TIME display will begin blinking Enter a value and press to set the value into memory The instrument automatically uses 25 C as the default temperature if another temper ature value has not been entered Then press and again to begin the run As the rotor begin
24. ccordance with the conditions listed below Drive replacement price for units not under service contract current drive exchange price i quotus labor and travel CONDITIONS The drive has been operated only within its rated speed and temperature ranges 2 The drive unit has not been subjected to unequal loading improper rotor installation corrosion from material spilled onto the hub or accumulated in the chamber of the instrument 3 The drive unit has not been disassembled modified or repaired except by Beckman Coulter personnel 4 The drive unit was installed by a Beckman Coulter Field Service representative 5 The instrument in which the drive unit has been used and operated and its associated rotors were manufactured by Beckman Coulter and serviced only by Beckman Coulter Field Service representatives If the above conditions are not met the full appropriate exchange price for the drive will be charged DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRAN TIES OF FITNESS AND OF THE WARRANTY OF MER CHANTABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHAT SOEVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT For details of drive coverage with a service contract contact your local Beckman Coulter service representative Beckman Coulter Wo
25. chamber If you hear a whining noise close the valve and wait as the rotor is still spinning 5 About 10 seconds after the hissing noise stops turn the screw to the left until it stops 4 3 Troublehshooting and Maintenance Top Panel Tabs Front Panel Access Port Front Panel Tabs Figure 4 2 Depressing the Latch to Loosen the Front Panel N WARNING NEVER try to slow or stop the rotor by hand 6 Push down on the interlock pin Figure 4 3 with your finger while opening the chamber door with your free hand If the rotor is still spinning close the door and wait 7 After retrieving your sample return the front panel to the instru ment To do so insert the tabs at the bottom of the front panel onto the lip at the base of the instrument being sure to align the front panel with the side panels 4 4 Troubleshooting and Maintenance Spring loaded Interlock Pin System Vacuum Solenoid Fan housing Figure 4 3 Interior View of Instrument Door Removed 8 Lift the front edge of the top panel a few inches and insert the upper edge of the front panel under it Push down gently to engage the tabs 9 Replace the control head by sliding it along the small ridges provided making sure to clear the emergency POWER OFF switch in front When the control head cover is correctly posi tioned push it until the latches snap shut and the cove
26. d Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the equipment Other symbols may also be displayed on the equipment These are reproduced and described under CONVENTIONS below and on the inside of the front cover Safety During Installation and or Maintenance This instrument is designed to be installed by a Beckman Coulter Field Service representative Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument Also should the instrument need to be moved a Beckman Coulter Field Service representative must reinstall and relevel the instrument in its new location Any servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury Make sure that the power switch is turned off and the instrument is disconnected from the main power source and refer such servicing to qualified personnel Do not replace any centrifuge components with parts not specified for use on this instrument Electrical Safety To reduce the risk of electrical shock this instrument uses a three wire electrical cord and plug to connect this equipment to earth ground Make sure that the matching wall outlet receptacle is properly wired and earth grounded Do not place containers holding liquid on or near the chamber door If they spill liquid may get into the
27. e 852 2814 7431 2814 0481 Fax 852 2873 4511 ITALY Beckman Coulter S p a Centro Direzionale Lombardo Palazzo F 1 Via Roma 108 20060 Cassina de Pecchi Milano Italy Telephone 02 953921 Fax 02 95392264 JAPAN Beckman Coulter K K Toranomon 37 Mori Bldg 3 5 1 Toranomon Minato ku Tokyo 105 0001 Japan Telephone 03 5404 8359 Fax 03 5404 8436 MEXICO Beckman Coulter de Mexico S A DE C V Avenida Popocat petl 396 Colonia Gral Pedro Maria Anaya Codigo Postal 03340 Mexico D F Mexico Telephone 525 605 77 70 Fax 525 575 18 25 NETHERLANDS Beckman Coulter Nederland B V Nijverheidsweg 21 3641 RP Mijdrecht Postbus 47 3640 AA Mijdrecht The Netherlands Telephone 0297 230630 Fax 0297 288082 SINGAPORE Beckman Coulter Singapore Pte Ltd 116 Changi Road Unit 03 01 02 Singapore 419718 Telephone 65 6339 3633 Fax 65 6336 6303 SOUTH AFRICA SUB SAHARAN AFRICA Beckman Coulter Stand 1A Primegro Park Tonetti Street 1685 Halfway House Johannesburg Republic of South Africa Telephone 27 11 805 2014 5 Fax 27 11 805 4120 e mail beckman intekom co za 2005 Beckman Coulter Inc SPAIN Beckman Coulter Espa a S A C Caleruega 81 28033 Madrid Spain Telephone 91 3836080 Fax 91 3836096 email bioresearch espana O beckman com SWEDEN Beckman Coulter AB Archimedesvaegen 7 Box 111 56 SE 168 11 Bromma Sweden Telephone 0 8 564 85 900 Telefax 0 8
28. fied S is used to generate centrifugal forces for the separation of particles The instrument can be used with all available Beckman Coulter rotors for standard and zonal operation This section describes the control panel and the instrument s major components A circuit breaker labeled I on and O off is located on the right side of the instrument and controls electrical power to the instrument Power may be left on at all times see STANDBY MODE below except in the case of an emergency or when maintenance is required A red POWER OFF button is provided on the front of the instrument for emergency use Pressing the POWER OFF button trips the circuit breaker to the down position To return power to the instrument the circuit breaker must be switched back up When the instrument is at rest it automatically goes into standby mode using only minimal power To restore the instrument to operating mode press any key and it becomes fully operational 1 1 Description KEY SWITCH POSITIONS A key interlock switch is used to select normal or zonal operation The normal position is used for routine closed door centrifugation and the zonal position for runs in which a zonal rotor is loaded and unloaded while spinning CONTROL PANEL Location of Diagnostic Messages Parameter Keys KEYS 1 2 Figure 1 1 shows the front control panel Refer to this illustration to locate the keys and displays described below Parame
29. il the required set of parameters appears To create or modify a program 1 Press PROG If no program has been previously selected a dash will appear above the key Actual values remain in the other displays 2 Use the keypad to select a program number which replaces the dash in the display Or continue pressing until the required program number appears The program number blinks to indicate that you are editing the program 3 Use the parameter keys and keypad to enter the run settings After all parameters have been entered press ENTER RECALL The SAVE indicator will begin to blink to indicate that you may save the program 4 Press and the program will be stored in memory under the selected number Actual values will reappear in the displays Run Preparation 2 8 ACTIVATION KEYS To select a program for use 1 Press PROG 2 Press the required program number 3 Press and START To modify a parameter during the run without changing the program change the setting as described above and then press ENTER RECALL Do NOT press SAVE The instrument will run using the modified settings The lighted number above the key will go out to indi cate that the instrument is no longer running from program memory Use the activation keys to control the following ultracentrifuge functions The vacuum system is activated automatically when you press and to begin a run To evacuate the chamber at another time m
30. ius rum dae e s EE hae Bi EEEE POE Ra X Preinstallation Requirements ccce xii DESCRIPTION POWED less E deso rek ye RR RE EREKE EE ERR e 1 1 Standby Mode sisse er Re hp 1 1 Key Switch Positions mess idee ke fase RR ru ae 1 2 Control Panel ers 252a x ead eed usa ed sd redd oes 1 2 KEYS herrees in eee ep OG he eee ai 1 2 Displays sese exit whale bo ette em eden wale 1 3 Run Status Indicators 2 0 eee eee 1 4 Diagnostic Messages eono eyso ap naaa saka aaa i 1 4 Doo sir tero mir tei Seal ea Ea Hr 1 5 Rotor Chamber Led ae ERR en e eg 1 5 Vacuuin System foo cata toe ec Seek eroe c a Pee ee 1 6 Temperature Sensing 00 eects 1 6 Temperature Control System 2 0 0 0 eee eee eee 1 6 Overspeed System 2 2 eR Re e KE eae a ed 1 7 Rotor Energy System 2 0 eee eee eee 1 7 DEY isa du wae seg th wise dt gdh Red ica ate acho Rie ei aces alae ees 1 7 Name Rating Plate ess iia cee eese cee Oba e ER eR ees 1 8 111 Contents iv SECTION 2 SECTION 3 SECTION 4 Warranty Page RUN PREPARATION POWER scans cene Been ORY Khe eR EEE EHE RE T ERE es 2 1 Key Switch Positions llle 2 1 Standard Operation 0 0 cece ee 2 2 Keypad sits ere ee eR Gabbe PORE EE ERE e E 2 2 Parameter Keys onem ree ex Ro 2 3 Programmed Operation 0 0 0 cece eee eee eee 2 7 Activation Keys outdoor eL dU b e naa dah ane 2 8 Delayed Start Program Function 0 000 e ee eee 2 10 Entering
31. ntrifuge is in the w2t mode HOLD HOLD indicates that the ultracentrifuge is in the HOLD mode PROG A number 1 through 9 above the program key indicates the number of the program that has been selected for the run SAVE SAVE blinks to indicate that you may save the program values just entered Press and the values will be saved in memory under the assigned program number VACUUM 750 200 and 20 microns located in the upper display indicate the approximate chamber pressure as the chamber is being evacuated Only one LED will be on continuously at any given time Once below 20 microns the 20 LED shines continuously until the key is pressed to vent the chamber The instrument will typically draw vacuum to 5 microns or less DIAGNOSTIC MESSAGES Diagnostic messages appear as red LEDs under the SPEED display see Figure 1 1 to alert you to conditions that may need your atten tion The indicators are not normally visible however if an abnormal condition occurs the appropriate message s will appear Description The meanings of the diagnostic messages as well as appropriate actions to take are discussed in Section 4 TROUBLESHOOTING AND MAINTENANCE DOOR The chamber door is made of high strength structural steel A solenoid interlock prevents it from being opened during operation The door can be opened only by using the door handle and only if the power is on and the vacuum is off with the chamber at atmospheric pre
32. o not run toxic pathogenic or other hazardous materi als in this instrument unless you take all appropriate safety precautions Ask your lab oratory safety officer to advise you about the level of containment required for your appli cation and the proper decontamination or sterilization procedures to follow if fluids escape from containers Make sure that such procedures will not damage the instrument see Section 4 for more detailed information and refer to Appendix A in Rotors and Tubes 3 1 Run Procedure The power must be turned on and the vacuum system turned off in order to open the chamber door STANDARD OPERATION 1 With the power on open the chamber door install the rotor and close the chamber door Press if you want to evacuate the chamber now 2 Enter the required run conditions SPEED TEMP and TIME HOLD or 21 3 Select ACCEL and DECEL profiles 4 Press and START The vacuum system is automat ically activated now unless you pressed VACUUM earlier Press to terminate a run in the HOLD mode or to stop any run for any reason Runs in the timed and 921 modes will terminate auto matically and the instrument will sound a tone to indicate the end of the run After the rotor has stopped press to vent the chamber Open the chamber door and remove the rotor To keep the rotor chamber clean and dry keep the door closed between runs REPEATING A RUN To repeat a run press and START There is
33. ogrammed operation Section 3 covers operating procedures for both kinds of runs Section 4 lists possible malfunctions together with probable causes and corrective actions and maintenance procedures We recommend that you read this entire manual especially the SAFETY NOTICE and all safety related information before operating or performing maintenance on this instrument vii Introduction I gt NOTE If the ultracentrifuge is used in a manner other than specified in this manual the safety and performance of this instrument could be impaired Further the use of any equipment other than that intended for use by Beckman Coulter has not been evaluated for safety Use of any equipment not specifically recommended in this manual is the sole responsibility of the user To obtain additional copies of this manual related rotor manuals and other referenced publications contact Beckman Coulter Inc Technical Publications Department 1050 Page Mill Road Palo Alto CA 94304 U S A Telephone 650 859 1753 Fax 650 859 1375 CONVENTIONS NOTES CAUTIONS AND WARNINGS Certain symbols are used throughout this manual to call attention to safety related and other important information The symbols used and their definitions are as follows gt NOTE Used to call attention to important information that should be followed during installation use and or servicing of this equipment N CAUTION Used to indicate a po
34. on regarding chemical resistance of instrument and accessory materials While Beckman Coulter has tested these methods and found that they do not damage the instrument no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods to use STORAGE AND TRANSPORTATION Contact Beckman Coulter Field Service for specific instructions and or assistance in preparing the instrument for transport or long term storage Temperature and humidity requirements for storage should meet the environmental requirements described under SPECIFICA TIONS at the front of this manual 1 Flammability hazard Do not use in or near operating ultracentrifuges SUPPLY LIST Troubleshooting and Maintenance Call Beckman Coulter Sales 1 800 742 2345 in the United States or your local Beckman Coulter office or refer to Ultracentrifuge Rotors Tubes amp Accessories catalog BR 8101 available at www beckman coulter com for information on ordering parts supplies rotors and accessories A partial list of supplies is given below for your convenience Silicone vacuum grease 1 OZ 0 0 cece mentis 335148 Spinkote lubricant 202Z 5 545 ese He Ses ERA ek a 306812 Beckman Solution 555 1 qt escana cece cee eee 339555 Chamber O rlfig ose Gs dag een tied en salen Mae dae te 801778 Logbook for preparative ultracentrifuges llle 3300
35. or imbalance Description NAME RATING PLATE A name rating plate is affixed to the rear of the instrument Always mention the serial number located on the chamber door and model number when contacting Beckman Coulter regarding your Optima L series instrument 1 8 2 e Run Preparation EHE Gusana ORO This section explains how to use the control panel to enter run infor mation Refer to Figure 1 1 for the location of keys and indicators POWER T If the power to the instrument is off flip the circuit breaker at the right side of the instrument on Do not turn the instrument power off except when maintenance is necessary or in the case of an emergency The instrument automatically goes into a standby mode when not in operation pressing any key makes the instrument fully operational once again KEY SWITCH POSITIONS Insert the key into the interlock on the control head to select normal or zonal operation The arrow on the key should point in the direction of the icon representing the selected operation These icons Figure 2 1 are displayed next to the interlock on the instrument Turn the key to the right to select the normal position for standard and programmed closed door centrifuga tion The key should be removed while the instrument is in this mode Turn the key to the left to select the zonal position for runs that require that the zonal rotor be loaded and unloaded while spinning The key CANN
36. pear in the PROG display When the time in program 0 reaches zero the second program will begin automatically If no second program was selected only program 0 will be run 3 3 Run Procedure ZONAL OPERATION A WARNING In zonal operation the operator is unavoid ably exposed to rotating machinery For safety the operator must be properly instructed and qualified Guard against accidentally dropping objects such as pens pencils or hemostats into the chamber Loose lab coats neckties scarves and long necklaces should not be worn while operating in the zonal mode Use only zonal rotors in the zonal mode Refer to the zonal rotor manual for detailed instructions on preparing and loading the rotor Then follow the instructions below to perform a zonal run 1 2 Precool the zonal rotor to the required temperature Turn the key left to select zonal operation The arrow on the key will point to the zonal icon This permits open door operation up to 3000 rpm Prepare the rotor assembly Install the rotor anti condensation shield and rotating shield assembly see the zonal rotor instruc tion manual Enter the required run settings speed temperature and time hold or w2t In the TIME and r modes the instrument will not begin counting time remaining until the chamber door is closed after the rotor has been loaded the vacuum level drops below 1000 microns and the rotor speed reaches 3100 rpm
37. r is aligned with the rest of the instrument panels N WARNING Do not attempt to run the instrument before returning the front panel to its correct position Troublehshooting and Maintenance DIAGNOSTIC MESSAGES 4 6 Diagnostic messages appear as red LEDs at the left side of the upper display see Figure 4 4 to alert you to conditions that may need your attention A tone will sound and the appropriate message will blink until you press the key The diagnostic messages will reappear if you attempt to restart the instrument and the problem has not been corrected TEMP DRIVE VAC DOOR PWR CPU Diagnostic Messages SPEED RPM TIME HR MIN od SES ES a ES ACCEL DECEL HOLD c SAVE 2 Optima mmc X 10 000 ata x POWER OFF o Figure 4 4 Location of Diagnostic Messages on the Control Panel Some of the messages provide cautionary information that will not shut down a run in progress Others indicate a user error For example if you left the chamber door open when you pressed START the DOOR message would appear to let you know it must be closed If the associated display is flashing when a diagnostic message appears a shut down malfunction has occurred The run will come to a stop See Table 4 1 to determine the nature of the error or problem possible causes and recommended corrective actions If no user action is indicated or the error persists call Beckman
38. re 1 Press TEMP The TEMP display blinks 2 Use the keypad to enter the required temperature If you enter an invalid value such as 60 C the instrument will beep twice Press to clear the value then enter a valid temperature 3 Press another parameter key or to save the set temperature The set temperature can be changed at any time by repeating the steps above The rotor temperature will be adjusted accordingly Actual rotor temperature after equilibration is controlled to 0 5 C of the set value If the temperature system malfunctions the TEMP diagnostic message will light and the rotor will decelerate to a stop Run Preparation The instrument has two acceleration profiles maximum MAX and Acceleration slow Press the key repeatedly to toggle between the settings The run status indicator for the selected setting will light The instrument has three deceleration profiles maximum slow and Deceleration no brake Press the key repeatedly to toggle between the settings The run status indicator for the selected setting will light PROGRAMMED OPERATION Up to nine sets of user specified run conditions called programs may Program be stored Each program is stored by number and can be recalled by and using the keypad Programs are retained in memory even when the power is turned off You can select an existing program by pressing the program number 1 to 9 on the keypad or by pressing the key repeatedly unt
39. ric device generates a series of pulses that are detected by electronic circuitry If the pulse rate which is dependent on the number of sectors on the disk and the speed of the rotor exceeds a set limit the SPEED diagnostic message will light and the rotor will automatically coast to a stop When the rotor reaches 1000 rpm the set speed is checked against the overspeed disk If the overspeed disk indicates a maximum allowable speed that is lower than the set speed the set speed is automatically lowered to the maximum speed permitted by the disk and a SPEED diagnostic message is displayed to alert you to the change In the t mode the time setting is recalculated to give an equivalent w2t value ROTOR ENERGY SYSTEM DRIVE 2 U S Pat No 4 846 773 As the rotor accelerates between 15 000 and 21 000 rpm rotor inertia is measured and the rotor energy is calculated for the speed set by the user If the calculated rotor energy is determined to be excessive a SPEED diagnostic message will appear and the instrument will immediately shut down The Optima L series ultracentrifuges use a frequency controlled air cooled direct drive induction motor that requires no gears or brushes In addition the drive does not require an oil vacuum seal external oil reservoir or continuously operating damper Externally cooled by forced air and internally cooled by oil the drive delivers ultrasmooth quiet performance with high tolerance of rot
40. rldwide Biomedical Research Division Offices AUSTRALIA Beckman Coulter Australia Pty Ltd Unit D 24 College St Gladesville NSW 2111 Australia Telephone 61 2 9844 6000 or toll free 1 800 060 880 Fax 61 2 9844 6096 email lifescienceaustralia 9 beckman com CANADA Beckman Coulter Canada Inc 6755 Mississauga Road Suite 600 Mississauga Ontario Canada L5N 7Y2 Telephone 905 819 1234 Fax 905 819 1485 CHINA Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center Jian Guomenwai Avenue Beijing 100004 China Telephone 86 10 6515 6028 Fax 86 10 6515 6025 6515 6026 EASTERN EUROPE MIDDLE EAST NORTH AFRICA Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 22 994 07 07 Fax 41 22 994 07 00 FRANCE Beckman Coulter France S A Paris Nord II 33 66 rue des Vanesses B P 50359 Villepinte France 95942 ROISSY CDG Cedex Telephone 01 49 90 90 00 Fax 01 49 90 90 10 e mail bioresearch france Obeckman com GERMANY Beckman Coulter GmbH Europark Fichtenhain B 13 47807 Krefeld Germany Telephone 49 21 513335 Fax 49 21 51333633 e mail bioresearch de 2 beckman com Deco COULTER Beckman Coulter Inc Sales and Service 1 800 742 2345 HONG KONG Beckman Coulter Hong Kong Ltd 12th Floor Oxford House 979 King s Road Taikoo Place Hong Kong Telephon
41. rpm press STOP again 3 5 Run Procedure 10 When the SPEED setting indicates that the rotor is spinning at 2000 rpm a series of beeps will sound press to vent the chamber Then open the chamber door UNLOADING 11 Unload the rotor according to the instructions in the zonal rotor manual Press after unloading to bring the rotor to rest Keep the door closed between runs to help keep the chamber clean and dry I gt NOTE When the rotor is at rest the instrument will automatically reset to the Normal mode POINTS TO REMEMBER Keep the chamber door closed whenever possible This helps to keep the chamber clean as well as assuring optimum performance of the vacuum system For fast temperature equilibration cool or warm the rotor to the required temperature before the run Refer to the applicable rotor manual for complete instructions on rotor preparation use and care Refer to Section 4 if a diagnostic message flashes on the control panel during the run 4 Troubleshooting and Maintenance This section lists possible malfunctions together with probable causes and corrective actions Maintenance procedures are also described For any problems not covered here contact Beckman Coulter Field Service for assistance I gt NOTE It is your responsibility to decontaminate the instrument as well as any rotors and or accesso ries before requesting service by Beckman Coulter Field Ser
42. s to accelerate the green LED on the key blinks When the rotor reaches set speed the LED stops blinking and remains on until the run has ended or is pressed Use the key to do the following Press at any time to terminate the run A green LED on the key will blink to indicate that the rotor is decelerating a tone will sound when the rotor comes to a stop and the green LED will turn off Press and within 5 seconds to display the run conditions that existed when the rotor began decelerating These are called the STOP values e Press ENTER RECALL STOP and in succession to display the accumulated 021 at the time the rotor began decelerating The w2t value is displayed in the SPEED display 2 9 Run Preparation DELAYED START PROGRAM FUNCTION ENTERING PROGRAM 0 The delayed start program function allows you the convenience of delaying the start of a run so that it begins and ends at the time you require without you having to be there to start the run For example if you require a four hour run to end at 8 00 a m you can program the instrument to hold the rotor at a specified temperature and time at zero speed and then automatically begin the run at 4 00 a m To use the delayed start program function a special program called program 0 is created followed by a second program that contains the run conditions Follow the instructions below The first program program 0 is used to delay the start of the run
43. ssure Refer to Section 4 for instructions on accessing the chamber to retrieve a sample in case of a power outage ROTOR CHAMBER The rotor chamber is made of aluminum coated with a chemical resistant epoxy finish The rotor drive spindle radiometer photoelec tric devices and safety plate are visible in the bottom of the chamber Figure 1 2 Photoelectric Devices Drive Spindle Radiometer Safety Plate Figure 1 2 The Rotor Chamber 1 5 Description 1 6 VACUUM SYSTEM The vacuum system is automatically activated when and are pressed or it may be turned on directly by pressing the key Three vacuum LEDs labeled 750 200 and lt 20 are located in the middle of the upper display to indicate the level of chamber pressure in microns At the end of a run the chamber vacuum must be vented by pressing VACUUM before the door can be opened After the door is opened the chamber is brought to approximate room temperature to prevent condensation from collecting in the chamber To help keep the chamber dry and clean the door should be kept closed whenever possible A purge system removes significant amounts of moisture from the vacuum pump and chamber If it takes a long time to pull a vacuum however it is likely caused by excess moisture in the system Refer to Section 4 for information on correcting this condition TEMPERATURE SENSING Rotor temperature is detected by a radiometer mounted in
44. t his or her own expense Introduction CANADIAN REGULATIONS This digital apparatus does not exceed the Class A limits for radio noise emissions from digital apparatus as set out in the radio interfer ence regulations of the Canadian Department of Communications Le pr sent appareil num rique n met pas de bruits radio lectriques d passant les limites applicables aux appareils num riques de Classe A prescrites dans le reglement sur le brouillage radioelectrique dict par le Minist re des Communications du Canada SETA SPECIFICATIONS CONTROL CHARACTERISTICS Only values with tolerances or limits are guaranteed data Values without tolerances are informative data without guarantee Speed Set speed 1000 rpm to maximum speed in increments of 100 rpm Speed control Actual rotor speed will be 20 rpm of the set speed above 1000 rpm Maximum speed will be 100 000 rpm or 90 000 rpm for the current models Models purchased earlier may include maximum speeds of 80 000 70 000 or 60 000 rpm Speed display Time Set time oooooo Time display Temperature Set temperature Temperature control Temperature display Temperature stability Ambient temperature t Integrator Set O20 ag cr ve pa ae t display Acceleration Deceleration Operation
45. t the instrument from the main power source and refer such mainte nance to service personnel N WARNING The following procedure should be imple mented only when absolutely necessary and only by qualified service personnel 1 Locate the two metal latches see Figure 4 1 at the lower outside corners on the back of the control head Use your fingers to depress the latches to disengage the cover from the control head Then with both hands slide the control head cover towards you until it is free Set the cover aside in a safe place 2 To remove the front panel use a long thin tool about 1 5 mm in diameter to depress the latch see Figure 4 2 While the latch is depressed use your other hand to lift the front edge of the top panel a few inches then remove the tool Allow the front panel to lean toward you 3 Lift the front panel to free the bottom tabs that secure the panel in place Troubleshooting and Maintenance Access to Cover Latch Cover Latch Remove Key Figure 4 1 Removing the Control Head Cover N WARNING After removing the panel listen carefully for any sounds coming from the drive Then touch the fan housing Figure 4 3 to feel if it is vibrating and listen again Do not proceed if any sound or vibration is emitted from the housing 4 Vent the vacuum chamber by slowly turning the screw on the vacuum solenoid valve to the right until you hear the air released into the
46. tentially hazardous situa tion which if not avoided may result in minor or moderate injury and or mechanical damage It is also used to alert against unsafe practices viii Introduction N WARNING Used whenever an action or condition may potentially cause serious personal injury or loss of life Mechanical damage may also result JN WARNING Indicates high voltage or risk of electric shock Refer servicing of all areas displaying this symbol to service personnel TYPOGRAPHIC CONVENTIONS Certain typographic conventions are used throughout this manual to distinguish names of user interface components such as keys and displays e Key names for example START or ENTER RECALL appear in capital letters within boxes Display names for example TEMP C or SPEED RPM appear in bold type RADIO INTERFERENCE This instrument has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial envi ronment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with this instruction manual may cause interference to radio communications Operation of this equipment in a residential area may cause interference in which case the user will be required to correct the interference a
47. ter Activation Keys Keypad Keys pR AS SPEED RPM TIME HR MIN zi VACUUM eo 000 0 aaa silices ACCEL DECEL HOLD c SAVE A Optima JO o ta fw CED Figure 1 1 The Control Panel Parameter keys are used to enter the run conditions They include SPEED TIME and to the left of the keypad and ACCEL DECEL a HOLD PROG and along the bottom of the display area DISPLAYS Actual Current Values Set Values Description Activation keys are designated to control specific ultracentrifuge functions These are VACUUM and STOP located to the right of the keypad All keys are discussed in more detail in the next section The keypad is used for entering numerical values In addition to keys 0 through 9 the keypad includes a Clear Entry key and key Digital displays indicate rotor speed run time rotor temperature the t value when in that mode and the selected program number if used The displays serve a dual purpose When the power is on the displays show the actual operating condi tions at all times except when the instrument is in the editing mode or when ENTER RECALL has been pressed see below Set values are the run conditions entered by the user When you press a parameter Key the associated display blinks to indicate that a value can be entered or modified This is referred to as the editing mode
48. ter will pay all trans portation charges Beckman Coulter makes no warranty concerning products or accessories not manufactured by it In the event of failure of any such product or accessory Beckman Coulter will give reasonable assistance to the Buyer in obtaining from the respective manufacturer whatever adjustment is reasonable in light of the manufacturer s own warranty Damage to the instrument while operating a rotor not of Beckman Coulter manufacture is not covered by warranty or service contract terms Further Beckman Coulter shall be released from all obligations under all warranties either expressed or implied if the product covered hereby is repaired or modified by persons other than its own authorized service personnel unless such repair is made by others who meet qualifications similar to those required of Beckman Coulter s service personnel or unless such repair in the sole opinion of Beckman Coulter is minor or unless such modification is merely the installation of a new Beckman Coulter plug in component for such product SPECIAL DRIVE WARRANTY During the instrument warranty period one year there will be no charge for drive replacement if the drive unit is installed serviced and operated in accordance with the conditions listed below During the drive s second through tenth year of use there is a prorated drive replacement price based on years of use if the drive unit is installed serviced and operated in a
49. th structural steel Diffusion pump in series with a mechanical pump typically reduces chamber pressure to below 5 microns 0 7 Pa xi Introduction xii Instrument Classification Diagnostic Messages PHYSICAL DATA S uses all Beckman Coulter preparative rotors except Types 35 and 42 1 rotors with serial numbers 1299 or lower See Section 4 TROUBLESHOOTING AND MAINTENANCE gt NOTE The Optima L series ultracentrifuges have been designed and tested to operate safely indoors at altitudes up to 2 000 m 6 562 ft AAPP esse eee SOG dos ew eed 465 kg 1025 Ib Height overall einst gly dir eene eR anes 120 7 cm 47 5 in Width voor dd 94 0 cm 37 in Dep ari rs 67 3 cm 26 5 in Ventilation clearances o oooooooooomoooo 5 1 cm 2 in sides 15 2 cm 6 in rear Finishes Keypad eoe tee eR coated polycarbonate A o Sd aai a x eas Ud S iE EUR urethane paint Other surfaces sisse eee meet acrylic baking enamel Maximum heat dissipation into the room 1 0 kW 3400 Btu hr Humidity restrictions 04 lt 95 non condensing Noise level measured 0 91 m 3 ft in front of the instrument lt 57 dBa Installation overvoltage category oooooooocoocncocr II Pollution degree i Ser cadena ete eR XU e ede Es UE V 2t Normally only nonconductive pollution occurs occasionally however a temporary conductivity caused by condensation must be expected Introduction PREINSTAL
50. until the required time Enter the settings as follows Press then 0 on the keypad to access program 0 A blinking zero will appear above the key The display area will show the existing settings for program 0 2 Use the parameter keys and keypad to enter time and temperature settings as required You cannot change the speed setting which is set at zero If you try to change it the instrument will signal an error 3 Press ENTER RECALL The SAVE message will blink to indicate that you can save the program settings 4 Press SAVE to save program 0 RUNNING PROGRAM 0 WITH A SECOND PROGRAM To run program 0 followed by another program do the following 1 Press then 0 on the keypad to access program 0 A blinking zero will appear above the key The display area will show the existing settings for program 0 2 Use the keypad to select a second program from the stored programs The program settings selected will appear in the display and the program number will appear in the display Press ENTERIRECALL 3 To begin the delayed start program function press and START Run Preparation The word delay will appear in the SPEED display and the second program number you selected will appear in the display When the time in program 0 reaches zero the second program will begin automatically Example To run programs 0 and 3 Press PROG 0 3 and ENTER RECALL Press and START EDITING
51. vice IN CASE OF POWER FAILURE DURING THE RUN If a power failure occurs during the run the rotor begins to decelerate with the brake off If the rotor is spinning above 1000 rpm when power is restored the instrument will resume operation and the rotor will return to set speed with maximum acceleration A PWR diagnostic message will appear to alert you that a power outage occurred If the rotor is spinning below 1000 rpm or has stopped when the power is restored the run automatically restarts If the instrument is in the TIME mode it will automatically reset the TIME display to the original set time In the HOLD mode the instrument will return to set speed and elapsed time will begin to accumulate again from zero A PWR diagnostic message will appear to alert you that a power outage occurred and that the run has been restarted automatically Troublehshooting and Maintenance RETRIEVING YOUR SAMPLE If a power failure lasts for several hours it may be necessary to retrieve your sample from the rotor A rotor decelerating without the brake may take hours to come to a complete stop To gain access to the rotor chamber under these circumstances you will need to remove the control head cover and the front panel to disengage the door lock N WARNING Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and or mechanical injury Therefore turn the power OFF and disconnec
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