User Manual - Galileo Bioscience
Contents
1. 80 0708 80 0708L 80 0911 80 1214 Gel Dimensions 7x8cm gasketed 7x8cm gasketed 9x11cm 12x14cm 7x10cm flush 7x10cm flush gasketed gasketed 12x14cm flush System 17 6x 12x 9 5 17 6x 12x 9 5 22x 15 x9 5 24 5 x 18x 9 5 Dimensions cm L x W x H Tray 2 comb slots 2 comb slots 2 comb slots 2 4 12 comb slots Configuration Comb thickness 1 0mm or 1 5mm polycarbonate Comb 5 6 8 10 and 12 5 6 8 10 and 12 5 8 10 12 14 8 16 20 24 MT9 configurations wells amp Prep Comb wells amp Prep Comb MT9 MT18 wells MT12 MT25 wells amp Prep Comb amp Prep Comb Voltage Limit 150 volts 150 volts 150 volts 150 volts Max Buffer 400 mls 400 mls 600 mls 800 mls volume CASTING THE GEL l Place the gel tray on a flat level surface It is important to pour the agarose gel on a level surface to prevent uneven migration of samples through the gel If casting tape is to be used tightly secure the tape over the open ends of the tray If using a gasketed tray place the tray into the chamber of the device so that the gasketed ends press against the walls of the buffer chamber Make sure that the gel tray is pressed all the way down and rests on the unit s gel tray platform 1 1 If the optional Adjustable Casting Platform is to be used ensure that the platform is level using the three point leveling system and place the tray into the casting platform with the open ends facin2. Resolve to Discover the syzygy of science art amp practicality GALILEO bioscience a SC x N ana MANUAL GALILEO bioscience Table of Contents Cover PAGO ERN GP OCR TOC Title Page Contact n isa abia 1 Warranty Component ustration pp 2 Packing p beet padre TANGE TEOT 2 Environmental Conditions Safety 3 Instructions For Use A Safety Precautions s MR General Care Cleaning sess D Specifications scare tos PC 6 Casting TRE T 6 OPE rino P 7 Vis alizatiO T fina 8 ligo REEL 8 References Do a Du 9 Appendix Agarose Preparation Notes pp 10 Comb Si 12 Aceessgnes t 14 NOTES 15 Copyright Galileo Bioscience 2004 TOC HORIZONTAL ELECTROPHORESIS GALILEO des i USER S MANUAL rev 0904 bioscience THIS MANUAL APPLIES TO THE FOLLOWING GALILEO BIOSCIENCE RAPIDCAST HORIZONTAL ELECTROPHORESIS SYSTEMS 80 0708 80 0708L 80 0911 80 1214 Ay mane A THESE UNITS ARE CAPABLE OF DELIVERING POTENTIALLY LETHAL VOLTAGE WHEN CONNECTED TO A POWER SUPPLY AND AR
3. 80 1214 SYSTEM SHOWN BELOW Gel Tray NL Buffer Chamber Table A INCLUDED SYSTEM COMPONENTS 80 0708 80 0708L 80 0911 80 1214 BUFFER CHAMBER 1 1 1 1 COVER W POWER 1 1 CORDS GEL TRAY GASKETED 1 1 1 1 GEL TRAY NON GASKETED 0 1 0 0 COMBS 2 2 2 2 Copyright Galileo Bioscience 2004 Page 2 ENVIRONMENTAL CONDITIONS FOR USE gt This unit is intended for indoor use only gt This unit can be operated safely at an altitude of 2 000 m The normal operating temperature range is between 49C and 659C gt Maximum relative humidity 80 for temperatures up to 319C decreasing linearly to 50 relative humidity at 409C SAFETY PRECAUTIONS Please read the User Manual carefully before using the Horizontal Electrophoresis Unit This manual contains important operating and safety information Our electrophoresis units are designed to perform flawlessly for years in the most demanding laboratories Please take the time to read the manual to ensure that you understand the safety and operating instructions to ensure the successful use of the unit Alterations could cause serious injury to the user or the system Power to the unit is supplied by an external power supply The power supply must meet safety standards for IEC 1010 1 regulations and must be ground isolated and incorporate a no load detecting circuit Power is supplied to the gel through the cover of the system providing a safety interl
4. gel use both comb slots on the tray Allow gel to solidify for 30 minutes Copyright Galileo Bioscience 2004 Page 6 1 Once the agarose has solidified carefully remove the casting tape from the gel tray or the gel tray from the casting platform If a gasketed tray is used carefully lift the tray out of the chamber turn it 90 and replace it into the chamber Remove casting comb s slowly to prevent any tearing of the wells NOTE If a high percentage agarose gel is cast it may be difficult to remove the casting comb from the tray If resistance is felt when removing the casting comb place the gel tray into the buffer tank and add buffer before removing the casting comb Place the tray onto the platform of the tank Be sure the gel tray is placed in the proper orientation in the tank the first comb slot should be closest to the black cathode electrode The banana plugs on the tank are color coded to help ensure proper orientation Pour the buffer into the tank Refer to the Appendix for buffer preparation The level of buffer should cover the gel by at least 2 3mm Check SPECIFICATIONS page 6 of this manual for buffer volume guidelines A fill line is located on the outside wall of the tank for easy reference Too little buffer may cause the gel to dry out during the run while excess buffer may slow DNA migration in the gel Dilute sample with appropriate volume of 10X Loading Buffer see Appendix for formul
5. AROSE PREPARATION NOTES Preparation amp Properties of TAE and TBE Electrophoresis Buffer Systems These buffers are used because they both have a basic pH that gives the phosphate group of the DNA a net negative charge allowing migration of the DNA toward the positive red anode in the gel unit TAE Tris Acetate with EDTA 40 mM Tris Base 40 mM Acetic Acid 1 mM EDTA 50X Stock Solution pH 8 5 1X Working Solution 242g Tris Base 40mM Tris Acetate 57 1 ml Glacial Acetic Acid 1mM EDTA 100 ml 0 5M EDTA pH 8 0 Distilled Water to 1 Liter Final Volume TBE Tris Borate with EDTA 89 mM Tris Base 89mM Boric Acid 2 mM EDTA 10X Stock Solution 1X Working Solution 108g Tris Base 89mM Tris Base 55g Boric Acid 89mM Boric Acid 20 ml 0 5M EDTA pH 8 0 2mM EDTA Distilled Water to 1 Liter Volume DO NOT UST pH Suggested Uses amp Comments TAE Buffer Use when DNA is to be recovered i e by low melting agarose gels Use for electrophoresis of large 220kb DNA fragments Applications requiring high resolution Under conditions with low ionic strength and low buffering capacity re circulation may be necessary for long runs gt hours TBE Buffer Use for electrophoresis of small lt lkb DNA fragments Better resolution of small 1lb DNA fragments Decreased DNA mobility High ionic strength and high buffering capacity no re circulation ne
6. E TO BE OPERATED ONLY BY QUALIFIED TECHNICALLY TRAINED PERSONNEL PLEASE READ THE ENTIRE OPERATOR S MANUAL THOROUGHLY BEFORE OPERATING THIS UNIT Galileo Bioscience P O Box 390566 Cambridge MA 02139 Toll Free 877 481 9175 Tel 781 481 9175 Fax 781 481 9214 www galileobioscience com Copyright Galileo Bioscience 2004 Page 1 WARRANTY Please check that the unit has been received complete and undamaged Refer to the illustration table below and check that all components are present Be sure to save all packaging and documents until you have thoroughly inspected your shipment if you find that your order is incorrect or damaged call for return instructions Galileo Bioscience Galileo guarantees that the Electrophoresis System you have received has passed rigorous quality assurance protocols and meets its published specification This warranty is valid for 36 months only if the product has been used and cared for according to this user manual No liability is accepted for loss or damage arising from incorrect use Galileo s liability is limited to the repair or replacement of the unit or refund of the purchase price at Galileo s option Galileo is not liable for any consequential damages Galileo reserves the right to alter the specifications of the Electrophoresis Systems without prior notice This will enable us to implement improvements as soon as they are available HORIZONTAL ELECTROPHORESIS SYSTEM COMPONENTS
7. NG Acrylic is not resistant to aromatic or halogenated hydrocarbons ketones or esters Organic solvents cause acrylic to craze or crack Do not use ethanol or other organic solvents to clean your unit Do not autoclave bake or microwave your unit A Before using clean and dry the unit Clean with DISTILLED WATER ONLY dry parts with clean lint free lab wipes or preferably air dry Use care when cleaning or drying the unit near the platinum wire The connectors should be clean and dry before usage or storage A Do not use abrasive creams or scourers A Do not use cleaning brushes in the electrode area A Athorough rinse with distilled water is all that is generally required to clean the unit after use A mild detergent may also be used Acrylic can also be exposed to a mild bleach solution 10 1 In addition RNAse removal products are also safe for acrylic See Page GEL TRAY All Galileo gel trays are made from UV transmissible acrylic which allows a stained agarose gel to be viewed directly in the tray on a transilluminator An incorporated fluorescent ruler aids in the calculation of relative mobility of DNA fragments NOTE care should be taken to avoid pouring freshly melted agarose directly into the gel tray as this may damage the tray Always allow agarose to cool to 60 C before pouring into the tray COMBS Combs for the Galileo Horizontal Electrophoresis Systems are available in thickness of 1 0mm and 1 5mm Please se
8. a Load samples A dark acrylic Well Visualization Guide is included with each Horizontal Electrophoresis System to allow a clear view of the wells when loading samples To use simply slide the Well Visualization Guide under the tank below the platform To load samples angle pipette tip into the well and slowly underlay sample into the well Take care not to push pipette tip through the bottom of the sample well as this will result in significant well to well leakage of samples Slide the cover onto the electrophoresis buffer tank insuring complete connection of the tank s banana plugs to the female connector ends of the power cords that are fixed to the cover The cover acts as a safety inter lock to prevent accidental shock during operation Attach the power cords to the power supply Turn on the power supply and set to the appropriate voltage level and begin electrophoresis Recommended running conditions are 5 volts cm of inter electrode distance Table C RECOMMENDED RUN CONDITIONS Model Inter electrode Distance Voltage 80 0708 80 0708L 18 cm 90V or 110volts constant 80 0911 19 cm 95V or 110 volts constant 80 1214 22 5 cm 112 5V or 110 volts constant 6 Run the gel for the appropriate amount of time for the specific sample being analyzed or until the dye front has migrated to approximately 5mm from the end of the gel Turn off power supply and detach the power cords Slide the cover back to remov
9. ay for 12cmW x 14cmL gels 4 comb slots 80 1214 RCGT12 RapidCast Gel Tray for 12cmW x 14cmL gels 12 comb slots 80 1214 RCGC RapidCast Caster only holds 3 Gasketed 12 x 14 cm trays 80 1214 RCPK RapidCast Caster Pack includes 3 80 1214 RCGT2 80 1214 GSK Replacement Gasket Pair 80 1214 UVT2 Flush Cut Gel Tray for 12cmW x 14cmL gels 2 comb slots 80 1214 UVT4 Flush Cut Gel Tray for 12cmW x 14cmL gels 4 comb slots 80 1214 UVT12 Flush Cut Gel Tray for 12cmW x 14cmL gels 12 comb slots 80 MADJ CST Mini Adjustable Horizontal Gel Caster accommodates gel trays 8 10 and 14 cm long up to 12cm wide 80 WADJ CST Maxi Adjustable Horizontal Gel Caster accommodates 3 8cm and 10cm long trays 2 12 x 14 cm long trays and 1 23cmW x 14cmL gel tray 80 1214 MT25 Sample Loading Guide for 25 tooth comb 12 x 2 plus 1 2X SLG micro titer format for improved reliability of loading with multi channel pipette Copyright Galileo Bioscience 2004 Page 14 Copyright Galileo Bioscience 2004 Page 15 Copyright Galileo Bioscience 2004 Page 16
10. e Lift the gel tray out of the buffer tank and proceed with visualization 7 It is important to rinse the tank with distilled water after every use to keep it clean It is recommended to allow the tank to air dry rather than drying with a wipe or towel to avoid damage to the electrode wires Copyright Galileo Bioscience 2004 Page 7 VISUALIZATION l Stain the gel with ethidium bromide solution or other suitable stain for agarose gels Note Ethidium bromide is a mutagen and can cause serious skin and eye irritation Wear gloves and safety glasses when handling 1 1 Ethidium Bromide Staining Once electrophoresis has been completed prepare 200ml of staining solution Add 10ul of 10mg ml ethidium bromide solution to 200ml de ionized water Soak the gel for 15 minutes with gentle shaking and then de stain in fresh de ionized water for 15 minutes Gel may then be viewed using a transilluminator The gel tray is made of UV transmissible acrylic and therefore may be placed directly on the transilluminator to view the gel 1 2 For quicker viewing of bands on the gel ethidium bromide solution may be added directly to the agarose gel before it is poured To use this method add ethidium solution to gel and to the running buffer in a concentration of 0 5ug ml TROUBLESHOOTING Many factors may affect the quality of gel preparations For example preparation of gel and sample buffers gel casting and tank assembly and or run condition
11. e Appendix Comb Specifications in the rear of this manual for details of combs available for each device Comb spines are acrylic and teeth are polycarbonate Combs designated MT are compatible for use with a multi channel pipettor Each comb is labeled with thickness number of teeth and catalog number BUFFER TANK The buffer tank holds the buffer and gel tray Current is passed from the power supply through the leads to the cover connectors through the platinum wires and into the buffer and from there into the gel The cover has incorporated safety rings that prevent human contact with the electrode connections to prevent electrical shock if the power supply is accidentally not turned off prior to removing the lid Users should not attempt to operate the system without the lid in place The electrode banana plugs are color coded red and black to ensure that the gel is placed in the correct orientation in the tank almost all electrophoretic separations of biomolecules run from the black electrode negative to the red electrode positive The connection between the banana plug and the electrode wire is sealed with a proprietary composite that eliminates corrosion of the connection The electrode wires are 0 254mm platinum and are secured to the inner walls of the tank Care should be taken not to damage the electrodes when cleaning the unit Copyright Galileo Bioscience 2004 Page 5 SPECIFICATIONS Table B GENERAL SPECIFICATIONS
12. eded for extended run times TBE Buffer reacts with the agarose making smaller pores and a tighter matrix This reduces broadening of the DNA bands for sharper resolutions Copyright Galileo Bioscience 2004 Page 10 Agarose Required volume of gel gel width x gel length x gel thickness Gel size cm x Gel thickness cm Gel Volume ml 7x8cm 0 25 cm 14 0 ml 7x8cm 0 50 cm 28 0 ml 7x8cm 0 75 cm 42 0 ml 7 x 10 cm 0 25 cm 17 5 ml 7 x 10 cm 0 50 cm 35 0 ml 7 x 10 cm 0 75 cm 52 5 ml 9 x 11 cm 0 25 cm 24 8 ml 9 x 11 cm 0 50 cm 49 5 ml 9 x 11 cm 0 75 cm 74 2 ml 12x14cm 0 25 cm 42 0 ml 12x14cm 0 50 cm 84 0 ml 12 x 14 cm 0 75 cm 126 0 ml Agarose percentage and separation of DNA fragments Percentage w v Agarose 0 Buffer ml Separation Range kb 0 3 0 15 50 5 60 0 5 0 25 50 1 30 0 7 0 35 50 0 8 12 1 0 0 50 50 0 5 10 1 2 0 60 50 0 3 7 1 5 0 75 50 0 2 4 2 0 1 00 50 0 1 3 3 0 1 50 50 0 1 Agarose Gel Loading Buffer Samples are prepared and combined with gel loading buffer before being loaded into the prepared gel Sample buffer usually contains similar components to the running buffer dyes for visibility and glycerol to provide some weight to the samples This increased sample density and color allows easy visualization of the samples and ensures samples load evenly into the wells and do not float out during loading Dyes also migrate toward the anode end of the electrophoresi
13. g the gasket Position the center hole of the sliding arm of the caster over the correct hole in the base and insert the cam into the hole in the arm and through to the hole in the platform with the cam pin facing the tray Rotating the cam pin 180 will force the tray forward and bring both gaskets into position on either side of the tray If the tray is correctly positioned with the gaskets it should not move when gently pushed from the side Prepare agarose for use in the device Refer to the Agarose Preparation Table in the Appendix for type and quantity of agarose needed be sure to reference the manufacturers instructions for any specific requirements for the agarose being used Weigh out the appropriate amount of agarose and empty into an Erlenmeyer flask Add buffer to flask TBE or TAE buffer are the most widely used buffer choices for DNA separations see Appendix for buffer formulations Heat agarose in microwave until all powder has gone into solution Swirl flask to be sure all agarose has dissolved IMPORTANT to avoid damage to UVT tray always cool agarose to 60 C before pouring into tray Any bubbles that form while pouring may be popped with a small glass pipette or the edge of the comb Place combs into the comb slots on the gel tray being careful not to create bubbles along the teeth of the comb If the entire gel length is required for separation place one comb in the first comb slot on the tray To separate more samples on one
14. m 0 5cm 0 75cm 1 0cm 80 0911 C5 1 0 5 1 0 15 4 25 47 68 80 0911 C8 1 0 8 1 0 9 0 20 38 55 80 0911 C10 1 0 10 1 0 6 8 14 26 38 80 0911 C12 1 0 12 1 0 5 4 11 19 28 80 0911 C14 1 0 14 1 0 3 7 8 15 22 80 0911 CMT9 1 0 9 1 0 7 2 16 30 43 80 0911 CMT18 1 0 18 1 0 2 7 6 11 16 80 0911 C5 1 5 5 1 5 11 3 38 70 102 80 0911 C6 1 5 6 1 5 9 1 31 56 82 80 0911 C8 1 5 8 1 5 6 4 22 40 58 80 0911 C10 1 5 10 1 5 4 7 16 29 42 80 0911 C12 1 5 12 1 5 3 7 12 23 33 80 0911 C14 1 5 14 1 5 80 0911 CMT9 1 5 9 1 5 7 2 24 45 65 80 0911 CMT18 1 5 18 1 5 2 7 9 17 24 80 0911 PREP 2 1 5 57 4 7 193 16 353 29 513 42 Copyright Galileo Bioscience 2004 Page 12 Galileo Comb ID No of Thickness Width Well volume teeth mm mm ul 0 5cm 0 75cm 1 0cm 80 1214 C8 1 0 8 1 0 12 5 28 52 75 80 1214 C16 1 0 16 1 0 5 4 12 22 32 80 1214 C20 1 0 20 1 0 3 9 9 16 23 80 1214 C25 1 0 24 1 0 3 0 7 12 18 80 1214 CMT9 1 0 9MT 1 0 7 2 16 30 43 80 1214 CMT12 1 0 12MT 1 0 7 2 16 30 43 80 1214 CMT25 1 0 25MT 1 0 2 7 6 11 16 80 1214 C8 1 5 8 1 5 12 5 42 77 113 80 1214 C16 1 5 16 1 5 5 4 18 33 49 80 1214 C20 1 5 20 1 5 3 9 13 24 35 80 1214 C24 1 5 24 1 5 3 0 10 19 27 80 1214 CMT9 1 5 9MT 1 5 7 2 24 45 65 80 1214 CMT12 1 5 12MT 1 5 7 2 24 45 65 80 1214 CMT25 1 5 25MT 1 5 2 7 9 17 24 80 1214 PREP Prep Comb 1 5 106 2 4 7 358 15 657 29 956 42 80 1214 WALL Wall Comb 1 5 Copyrigh
15. ns Problem One side of gel running slower gt Gel may be uneven Be sure to pour gel on a level surface gt Check integrity of electrodes to be sure no breakage has occurred Any breaks in the electrode may cause uneven migration or none at all If a break is found contact Galileo Problem No bands visible gt Be sure gel was placed in the electrophoresis tank in the proper orientation If orientation or polarity is reversed the samples will migrate off the gel f DNA marker was used and is present on the gel check the integrity of the sample gt Shorten run time Gel should be stopped when the dye front has migrated to approximately 5mm from the end of the gel Additional sources for Reference Maniatis T E F Fritsch and J Sambrook Molecular Cloning A Laboratory Manual Cold Spring Harbor Laboratory Cold Spring Harbor NY Short Protocols in Molecular Biology A Compendium of Methods from Current Protocols in Molecular Biology Edited by Frederick M Ausubel et al Adams D and R Ogden Electrophoresis in Agarose amd Acrylamide Gels Methods in Enzymology Vol 152 1987 Academic Press Inc Fotador U Simultaneous Use of Standard and Low Melting Agarose for the Separation and Isolation of DNA by Electrophoresis BioTechniques Vol 10 No 2 1991 Boots S Gel Electrophoresis of DNA Analytical Chemistry Vol 61 No 8 April 15 1989 Copyright Galileo Bioscience 2004 Page 9 APPENDIX AG
16. ock to the user Users should not attempt to operate this unit without the safety inter locked cover in place A Always disconnect the unit from the power supply before removing cover to avoid the risk of personal shock A Running Conditions should not exceed the maximum operating voltage or current A Do Not fill the Buffer Chamber with running buffer above the maximum fill line A Always disconnect the unit from the power supply when you want to move the unit or add running buffer A Use this apparatus only for its intended purpose as described in this manual Do not use this product if the power cords are damaged or if any of its surfaces are cracked Copyright Galileo Bioscience 2004 Page 3 GALILEO RAPIDCAST GALILEO HORIZONTAL bioscience INSTRUCTIONS FOR USE INTRODUCTION Thank you for your purchase of a Galileo RapidCast Horizontal mini gel electrophoresis system The Galileo RapidCast horizontal electrophoresis devices allow quick separation and analysis of nucleic acids on an agarose gel Gels can be cast in a variety of ways Use the gasketed trays directly in the buffer chamber Use multiple gasketed trays in optional casting trough Tape ends of trays Use one of the optional Adjustable Casting Platforms The UV transparent gel trays are available with a variety of comb slot configurations Combs are available in 1 0mm and 1 5mm thickness as well as a variety of tooth configura
17. s Reading and following the instructions in this operating manual can solve most problems Below we list some of those most commonly experienced problems along with suggestions for solving them Problem Gel Smiling gt Gel may be uneven Be sure to pour gel on a level surface Voltage may be too high Lower voltage setting on power supply See Instructions for proper voltage Problem No migration gt No power is contacting the gel Be sure all contacts are made between the power supply and the electrophoresis tank gt Check buffer preparation to be sure the proper ionic strength buffer was prepared gt Check integrity of electrodes to be sure no breakage has occurred Any breaks in the electrode will cause slow migration or none at all If a break is found contact Galileo Problem Slow migration gt Check buffer preparation to be sure the proper ionic strength buffer was prepared Voltage too low increase voltage see Instructions for proper running conditions gt Check integrity of electrodes to be sure no breakage has occurred Any breaks in the electrode will cause slow migration or none at all If a break is found contact the Galileo Problem Excessive heat gt lonic strength of buffer is too high Check buffer preparation to be sure the proper ionic strength buffer was prepared Copyright Galileo Bioscience 2004 Page 8 Voltage istoo high decrease the voltage see Table C for proper running conditio
18. s chamber at predictable rates allowing the gel run to be monitored The most commonly used loading buffer is glycerol bromophenol blue and xylene cyanol 6x loading buffer for agarose gels 0 25 bromophenol blue 0 2596 xylene cyanol FF 3096 glycerole in water Copyright Galileo Bioscience 2004 Page 11 COMB SPECIFICATIONS NOTE Well volumes are calculated assuming a 0 5cm thick gel Since the comb placement leaves 0 2 cm of agarose under the sample well a 0 5 cm thick gel will have sample well that is 0 3 cm deep a 1 cm thick gel would leave a sample well depth of 0 8 cm Loading Volume is calculated as Thickness of Tooth x Width of Tooth x Depth of Sample gel thickness 0 2cm x 75 MODEL 80 0708 amp 80 0708L Galileo Comb ID Number Thickness Width Well volume ul teeth mm mm 0 5cm 0 75c 1 0cm m 80 0708 C5 1 0 5 1 0 11 3 25 47 68 80 0708 C6 1 0 6 1 0 9 1 20 38 55 80 0708 C8 1 0 8 1 0 6 4 14 26 38 80 0708 C10 1 0 10 1 0 4 7 11 19 28 80 0708 C12 1 0 12 1 0 3 7 8 15 22 80 0708 C5 1 5 5 1 5 11 3 38 70 102 80 0708 C6 1 5 6 1 5 9 1 31 56 82 80 0708 C8 1 5 8 1 5 6 4 22 40 58 80 0708 C10 1 5 10 1 5 4 7 16 29 42 80 0708 C12 1 5 12 1 5 3 7 12 23 33 80 0708 PREP 2 1 5 57 4 7 193 16 353 29 513 42 MODEL 80 0911 Galileo Comb ID Number Thickness Width Well volume ul teeth mm m
19. t Galileo Bioscience 2004 Page 13 ACCESSORIES AVAILABLE FOR GALILEO HORIZONTAL UNITS RapidCast 80 0708 80 0708L 80 0708 RCGT RapidCast Gel Tray for 7cmW x 8cmL gels 2 comb slots 80 0708 LGT Flush Cut Gel Tray for 7cmW x 10cmL gels 2 comb slots 80 0708 RCGC RapidCast Gel Caster only will hold 1 3 80 0708 RCGT 80 0708 RCPK RapidCast Gel Caster Pack includes 3 80 0708 RCGT 80 0708 GSK Replacement Gaskets for 80 0708 RCGT 2 80 MADJ CST Mini Adjustable Horizontal Gel Caster can be used with 8 10 11 14 cm Long Gel Trays up to 12cm Wide 80 WADJ CST Wide Adj ustable Horizontal Gel Caster can be used with 8 10 11 14 amp 17 cm Long Gel Trays up to 23 cm Wide RapidCast 80 0911 80 0911 RCGT 80 0911 RCGC 80 0911 RCPK RapidCast Gel Tray for 9cmW x 11cmL gels 2 comb slots RapidCast Gel Caster only will hold 1 3 80 0911 RCGT RapidCast Gel Caster Pack includes 3 80 0911 RCGT 80 0911 GSK Replacement Gaskets for 80 0911 RCGT 2 80 MADJ CST Mini Adj ustable Horizontal Gel Caster can be used with 8 10 11 14 cm Long Gel Trays up to 12cm Wide 80 WADJ CST Wide Adj ustable Horizontal Gel Caster can be used with 8 10 11 14 amp 17 cm Long Gel Trays up to 23 cm Wide RapidCast 80 1214 80 1214 RCGT2 RapidCast Gel Tray for 12cmW x 14cmL gels 2 comb slots 80 1214 RCGT4 RapidCast Gel Tr
20. tions to accommodate varying numbers of samples and sample volumes Outstanding Features Ensure Trouble Free Use Robust Acrylic Construction Stands up to Daily Usage Without Breakage Warping or Leakage All Galileo Bioscience Horizontal Electrophoresis Systems Are Hand Fabricated Providing Unmatched Durability For Years Of Daily Use Systems Meet or Exceed All Safety Requirements For The IEC1010 1 Standards Units Accommodate Gasketed Gel Trays Allowing Casting Of Gels In The Buffer Chamber Without Tape Dams or External Casting Stands USING HORIZONTAL GEL ELECTROPHORESIS UNITS gt gt P amp P ee Pe SAFETY PRECAUTIONS READ all instructions before using the unit ALWAYS turn off power supply FIRST then disconnect the power cords Always have electrophoresis unit disconnected from their power supply before removing the safety cover DO NOT exceed the maximum operating voltage or current see TABLE B DO NOT operate electrophoresis units in metal trays DO NOT fill the unit with running buffer above the maximum Fill Line DO NOT move the unit when it is running CAUTION During electrophoresis very low quantities of various gases are produced at the electrodes The type of gas produced depends on the composition of the buffer employed To disperse these gases make sure that the unit is run in a well ventilated area Copyright Galileo Bioscience 2004 Page 4 GENERAL CARE amp CLEANING WARNI
Download Pdf Manuals
Related Search