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User`s Manual - Leica Biosystems

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1. blade holder with base and select the desired clearance angle Attach the cable holder to the microtome cable holders are included in the standard delivery of the RM CoolClamp 14 Attaching to the Rotary Microtome of the Leica RM22 23 Series with Quick Change System INHECO GmbH 4 Attaching to Rotary Microtomes of the Leica RM22 23 Series with Quick Change System The RM CoolClamp is attached the same way as changing the universal cassette clamp for additional information also refer to the operating manual of the respective rotary microtomes There are two versions of the specimen holder fixture one with and one without specimen orientation The two versions can be interchanged The specimen orientation allows for simple position correction of the specimen surface when the specimen is clamped into place To do so proceed as follows Move the specimen holder fixture 60 to the upper end position by turning the handwheel 12 and engage the handwheel lock release the clamping system turn the screw 61 counterclockwise using a size 4 Allen key Push the guide 63 of the RM CoolClamp 62 from the left into the specimen holder fixture 60 as far as it will go clamp the specimen clamp turn the screw 61 clockwise as far as it will go Figure 6 Attaching the RM CoolClamp 15 Fine Adjustment of the Force Balance 5 Fine Adjustment of the Force Balance Installing the RM CoolCl
2. input power 18W Nominal frequenc 47 to 63 Hz Maximum output power 22 5 W Output operating voltage 7 5 V DC Operating temperature 10 to 35 C 50 F to 95 F relative humidity 10 to 80 non condensing Transport and storage conditions 10 to 60 14 to 140 F relative humidity 10 to 80 non condensing elevation 0 2000m Temperature difference between 20K 3K operating temperature and tensioning clamp LxWxH with power pack plug Dimensions 80mm x 115mm x 205mm Weight without power pack but with 0 65 kg plug Table 1 Technical Data 18 INHECO GmbH Declaration of Conformity 8 Declaration of Conformity INHECO GmbH Fraunhoferstr 11 82152 Martinsried Germany Confirms that the following product RM CoolClamp fulfills the essential requirements of the following directive s and their relevant part s of the standards and their related documents of the European Union Standards and date of issue EN 61010 1 2004 oafety requirements for electrical UL 61010 1 2004 equipment for measurement control CSA C22 2 61010 1 2004 and laboratory use Part 1 General requirements diagnostics instruments EMC requirements EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements This product fulfills the directives 98 79 EC for in vitro diagnostics medical devices 2002 96 EC WEEE and 2002 95 EC RoHS Martinsried Munich Da
3. ORK BE CARRIED OUT BY THE 17 7 iat ge DRE E ac REPE ERE EXER Eten 18 8 DECLARATION OF CONFORMILIY cieieein inen bue EUR OSEE era cep ee Ene ue 19 APPENDIX A ABBREVIATION GLOSSATRLUY petu a3 qe ee ak ER eEeEE ER eua Eo sce Duke 20 a 20 APPENDIX C TABLES INDEX CUBE cu s in c du 20 APPENDIX D DOCUMENT HISTORY pev E PR 20 INHECO GmbH INHECO GmbH Symbols in the Text and Their Meanings 1 Symbols in the Text and Their Meanings Notice Warning Safety hazard Warning of a potential hazard with a low risk of injury This symbol also warns you of property damage This symbol indicates useful information for installation etc This arrow is for indicating steps and bullet points A A A A A A A A D INHECO GmbH Safety Notes 2 Safety Notes General use The RM CoolClamp complies with the current technical requirements The manufacturer has placed the greatest value on user safety The following rules apply for the user Rules for accident prevention General rules for technical safety The respective specific directives of the EU and other countries G
4. amp necessitates performance of a force balance for additional information also refer to the operating manuals of the respective rotary microtomes If another accessory of a different weight is mounted on the specimen head 33 you must check whether it is necessary to readjust the force balance Checking the correct setting Attaching a new accessory and clamping a specimen Set the specimen head to half the height of the vertical travel range by turning the handwheel Figure 7 9 00 position If the specimen head remains in this exact position the setting is correct If the specimen head moves i e it is raised or lowered fine adjustment is necessary Failure to adjust the force balance may result in injury while working see the Safety Notes on page 8 The force balance is adjusted using the screw 34 which can be accessed by removing the section waste tray on the bottom of the base plate of the microtome Use the Allen key provided size 5 with handle for the adjustment Ifthe specimen head moves downwards turn the screw 34 clockwise Ifthe specimen head moves upwards turn the screw 34 counterclockwise Continue this procedure until the specimen head no longer moves once released Figure 7 Fine adjustment of the force balance INHECO GmbH 16 INHECO GmbH Maintenance Work to be Carried Out by the User 6 Maintenance Work to be Carried Out by the User Cleaning Before beginn
5. ectioned must be precooled to 5 C 23 F using ice or a cooling plate prior to sectioning After the RM CoolClamp s precooling phase of 30 min the universal cassette can be inserted and the sections carried out Permitted maximum sectioning speed 420 mm s Section thickness range 0 5 600 um Operating concept Installation on a rotary microtome of the RM22 23 series The following applies in general Handling specimen head or knife blade holder always lock handwheel in place Release locking mechanism only for sectioning and use knife guard see Safety Notes on page 8 For a microtome that is already configured remove knife blade holder with base and specimen clamp Bring the specimen head into the rear end position home position Lock the handwheel in place and where appropriate check whether the handbrake has been activated We recommend working with the non directional specimen holder fixture if you will be working with the directional specimen fixture XY or fine it must be brought into the zero position Push the RM CoolClamp with adapter into the dovetail guide of the quick change system for specimen clamps and tighten it using a size 4 Allen key To carry out the force balancing please observe the operating manual of the respective microtome under the Operation chapter Fine Adjustment of the Force Balance The specimen head must not fall into the knife after being stopped released Reinstall the knife
6. eneral use includes handling according to the operating manual Operation of this unit is intended only for dry ambient conditions without precipitation of liquids Defective instruments must not be used It must be possible for the user to shut off the power supply to the instrument via the power supply unit instantly if necessary following applies in general Handling specimen head or knife blade holder always lock handwheel in place Release locking mechanism only for sectioning and use knife guard Failure to adjust the force balance may result in injury while working The unit must not be operated in rooms with danger of explosion The RM CoolClamp must be operated only in the installation orientation and position prescribed for it It must be ensured that no liquids ingress into the unit instrument may be operated indoors only To keep the thermomechanical stress on the TEC as minimal as possible the RM CoolClamp should be switched on only once per day and then remain in operation Who is permitted to operate this unit Only instructed and trained personnel are permitted to operate this unit INHECO GmbH oafety note Intended use The RM CoolClamp is an electric cooling specimen holder for the universal cassettes and the paraffin embedded specimens in the cassettes It is attached with the specimen holder to the rotary microtomes of the RM22 23 series Any other u
7. entilation openings outlet Ventilation openings inlet Figure 2 Ventilation openings When operating the RM CoolClamp condensation on the clamp occurs This is a natural physical process INHECO GmbH 11 INHECO GmbH Instrument Setup The cable of the RM CoolClamp must be routed to prevent possible severing of the cable when operating the microtome For this purpose attach the cable holder to the microtome and then insert the cable into the cable duct Cable holder position 20 cm high 6 cm lateral gt Figure 3 Position of the cable duct 12 Instrument Setup It is possible for fingers to become pinched in the specimen holder in the event of improper handling Potential pinching areas Figure 4 Potential pinching areas Select the appropriate adapter for the power supply To insert or remove the corresponding adapter the unlocking button must be pressed Figure 5 Power pack with EU UK USA and Australia adapters INHECO GmbH 13 INHECO GmbH Instrument Setup Prerequisite for sectioning the specimens Prerequisite for using the RM CoolClamp To use it with the rotary microtomes of the RM22 23 series a vertical travel range of at least 70mm and an adjustable force balance are necessary To use the non directional specimen fixture or the directional specimen fixture fine or only XY bring the specimen fixture into the zero position The embedded tissue samples to be s
8. ing to clean the RM CoolClamp make sure that the power pack has been unplugged and all surfaces have cooled down to below 50 C 122 F Clean the RM CoolClamp using paraffin remover only Xylene and other solvents are not permitted Wait to reactivate it until all the liquid has evaporated Make sure that no liquid ingresses into the instrument ventilation slots Use only enough liquid as is absolutely necessary The assembly group is not suited for cleaning by dipping into liquid To guarantee that the RM CoolClamp functions flawlessly make sure that the fan rotates and is not blocked In addition make sure that the ventilation openings are free before and during use see page 11 No cleaning in the incubator is permitted The RM CoolClamp must not be disassembled for cleaning Decontamination No units may be sent back to INHECO if contaminated Potential harm to the recipient s health must be precluded The most common decontamination method is disinfection using formaldehyde or ethylene oxide gas The surface decontamination may include wiping the surface A solution of 70 alcohol is the conventional method for effectively removing organisms Make sure that no moisture gets inside the unit Spare parts Spare parts for the unit must be ordered through Leica Biosystems Nussloch GmbH Only original parts are permitted for use 17 INHECO GmbH Technical Data 7 Technical Data Operating voltage 7 5V DC Maximum
9. inheco industrial heating amp cooling RM CoolClamp Electronically Cooled Universal Cassette Clamp INHECO article number 7900100 LEICA article number 14 0502 46537 Operating Manual Revision status V1 1 July 2011 Always keep this manual with the instrument Read carefully before working with the instrument BA RM CoolClamp 11 07 15 7900100RM CoolClamp V1 1 English doc Printed in Germany INHECO GmbH RM CoolClamp INHECO GmbH reserves the right to modify its products in order to improve their quality These modifications are usually not documented This operating manual and the information contained in it have been compiled according to the best of our knowledge INHECO GmbH assumes no liability in the event of printing errors or damage that is caused by printing errors Trademarks and product names in this operating manual are registered brand names and each belongs to the corresponding owner Contact Contact INHECO GmbH Fraunhoferstr 11 82152 Martinsried Germany Phone Sales 089 899593 101 Phone Technical Hotline 089 899593 201 Fax 089 899593 499 E mail Sales sales inheco com E mail Technical Hotline techhotline inheco com Internet www inheco com This manual belongs to To be filled out by the customer Series Serial number Year of manufacture Order number PRODUCT SERVICE Inventory number Location of instrument setup INHECO GmbH INHECO GmbH RM CoolClam
10. ng is accomplished by replacement No repairs may be carried out by the user Disposing of the unit Dispose of the unit in accordance with the respective environmental directives of the respective country In the event of contamination comply with the safety instructions The RM CoolClamp is RoHS compliant Instrument Setup 3 Instrument Setup Standard delivery Prior to instrument setup make sure that the shipment is complete and no part is damaged The following components should be included in each shipment RM CoolClamp Power pack with EU UK USA and Australia adapters Cable holders for attaching to the microtome not shown in the figure Operating manual as a book with 3 languages and on CD with 16 languages not shown in the figure The RM CoolClamp can be used only together with the quick change system on the rotary microtomes of the Leica RM22 23 series Figure 1 Standard delivery INHECO GmbH 10 Instrument Setup General operating instructions To prevent injury and damage provision must be made for an unobstructed air supply refer also to the cleaning instructions on page 17 The fan must not be blocked and the air outlet must not be obstructed Please check before each use that the fan rotates freely To prevent damage to the RM CoolClamp the maximum permissible ambient temperature must not be exceeded for additional information refer to the specifications on page 18 V
11. p Significance of this Operating Manual This operating manual is part of the RM CoolClamp and must gt remain at the RM CoolClamp until its final destruction passed on if the RM CoolClamp is sold or lent Please contact the manufacturer in the event that you do not understand the operating manual or portions of it Your opinion regarding the operating manual gives us valuable insights into how we can continue to serve you best Please do not hesitate to send us your remarks see the address or telephone numbers on page 4 The safety instructions must be read with the greatest care and attentiveness They must be understood and observed in order to ensure safe handling of the instrument Insufficient or lacking knowledge of the contents of the operating manual relieves INHECO GmbH of liability Therefore the operator should request an orientation from the distributor Leica Biosystems Nussloch GmbH Contents Contents 1 SYMBOLS IN THE TEXT AND THEIR MEANINGS eren 7 2 SAPFEIY NOTES ee nee ese nee Re en ees RE oe OR Om D SS nea CAD 8 3 INSTRUMENT SETUP 10 4 ATTACHING TO ROTARY MICROTOMES OF THE LEICA RM22 23 SERIES WITH QUICK CHANGE SYSTEM rein reete e asso p 15 5 FINE ADJUSTMENT OF THE FORCE 222 1 3 nnn 16 6 MAINTENANCE W
12. se is improper and therefore not permitted Technical modifications For safety reasons no technical modifications to this instrument are permitted Any change or modification that the manufacturer has not approved causes the warranty to be voided Original parts are specially designed for the RM CoolClamp Parts from other manufacturers have not been tested and therefore have not been approved and permitted by INHECO GmbH No liability shall be accepted for any use other than the intended use Biosafety in the laboratory environment When using the RM CoolClamp in a laboratory environment with biosafety the user is responsible for labeling the RM CoolClamp according to the WHO Laboratory Biosafety Manual ISBN 92 4154650 6 In addition when using the RM CoolClamp the user is responsible for adhering to the rules and regulations depending on the biosafety level with respect to the WHO Laboratory Biosafety Manual The safety rules and regulations in force for the respective lab must be monitored constantly while work is being carried out with the unit Malfunctions Report any malfunctions that occur to the person responsible for the instrument immediately Make sure that the unit has been secured against misuse and abuse Any safety related parts that have been removed must be reattached and checked prior to instrument setup gt servicing the RM CoolClamp The unit is not intended to be serviced Servici
13. te 7 2010 Signature G nter Tenzler Managing Director 19 INHECO GmbH Appendix Appendix A Abbreviation Glossary Table 2 Abbreviation Glossary Appendix B Index of Figures Figure 1 Standard delivery 10 Figure 2 Ventilation openings 11 Figure 3 Position of the cable duct 12 Figure 4 Potential pinching areas 13 Figure 5 Power pack with EU UK USA and Australia adapters 13 Figure 6 Attaching the RM CoolClamp 15 Figure 7 Fine adjustment of the force balance 16 Appendix C Tables Index Table 1 Technical Data 18 Table 2 Abbreviation Glossary 20 Appendix D Document History Version Date 05 05 2010 Expanded standard delivery 7 20

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