DPA-2FX Manual - NPI Electronic Instruments
Contents
1. D Electronic Instruments for the Life Sciences pAs e ade OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE DPA 2FX DIFFERENTIAL AMPLIFIER FILTER MODULE FOR EPMS SYSTEMS DPA 2FX npi OEESET VERSION 1 8 npi 2014 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com DPA 2FX User Manual Table of Contents l A TRI 3 2 EPVS 07 Modular Plue la Syste MM lili 4 2 1 General System Description Oper dat 4 O INI HO 7 TOUS PA on A 4 235 BRNS E 07 HOUSE ica 4 DAS PWR OSD A a 4 2 5 SyS TOUS ii 5 EPM S OTi E A A T A 5 ERMS T Oea r a Nm A a err 5 2 05 Tecimcal Dalis N isis ciao ide 5 E et fe e Y PA E E arse na E E E orate aa E A A E E EAT 5 PES TEO OR O 5 3 DPA 2FX Differential Amplifier Filter Module oooooooooononnnnnncnnnnccnnnnnnnnnnnnnnnnononos 6 Sal DRAZEX Component aia 6 ANSIA DES CUP UO Ree eer re ea ee E em ee on 6 3 3 VIERA FlOW DIAM E 6 3 4 Description of the Front Panel and Operat10D occccnccccnnonnnonnnnnnnnnonononnnnnnnnnnnnnnanannnonss 7 o 2 HE SO 10 Den CC IMC AN da a da 11 version 1 8 page 2 DPA 2FX User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life support2. H 225 mm x 210 mm x 85 mm version 1 8 page 5 DPA 2FX User Manual 3 DPA 2FX Differential Amplifier Filter Module 3 1 DPA 2FX Components The following items are shipped with the DPA 2FX system Y Amplifier Filter module for the EPMS 07 system Y User manual 3 2 System Description The DPA 2FX differential amplifier filter 1s a plug in unit for the npi EPMS 07 modular system The DPA 2FX is designed to amplify and filter small bio electrical signals The input voltage range is 12 V the inputs are protected up to 15 V 2 overload LEDs indicate if the linear region of the amplifier is exceeded All inputs and the output are BNC connectors The input impedance is 10 MQ Thus the module can be used for direct recording of bioelectric signals e g from peripheral nerves Using the differential input configuration common mode signals and noise are removed allowing recordings of signals with small amplitudes DC offsets of the signal can be compensated either by the using the OFFSET control or they can be eliminated using the AC position of the input switches or by using the HIGHPASS filter DC offsets from the amplifier can be corrected by two trimpots The GAIN range is x1 to x10000 and can be set with two switches 1 2 5 10 20 50 100 rotary switch and 1 10 100 range switch The output signal is passed through the HIGHPASS and LOWPASS filter Important To avoid noise interference the respective toggle switch should be in th
3. 3 Changes in intracellular calcium transients and LTP in the surround of visual cortex lesions in rats Brain Res 990 120 128 _I Boulton A A Baker G B amp Vanderwolf C H eds 1990 Neurophysiological Techniques Basic Methods and Concepts Humana Press Clifton New Jersey L Huemmeke M Eysel U T amp Mittmann T 2002 Metabotropic glutamate receptors mediate expression of LTP in slices of rat visual cortex Eur J Neurosci 15 1641 1645 LJ Huemmeke M Eysel U T amp Mittmann T 2004 Lesion induced enhancement of LTP in rat visual cortex is mediated by NMDA receptors containing the NR2B subunit J Physiol 559 875 882 _I Schulz D Huston J P Jezek K Haas H L Roth Harer A Selbach O amp Luhmann H J 2002 Water maze performance exploratory activity inhibitory avoidance and hippocampal plasticity in aged superior and inferior learners Eur J Neurosci 16 2175 2185 Y Kettenmann H amp Grantyn R eds 1992 Practical Electrophysiological Methods Wiley Liss New York LI Lalley P M A K Moschovakis and U Windhorst 1999 Electrical Activity of Individual Neurons In Situ Extra and Intracellular Recording in U Windhorst and H Johansson eds Modern Techniques in Neuroscience Research Springer Berlin New York LI M ller Ch M 1992 Extra and Intracellular Recording in the Slice in Kettenmann H amp Grantyn R eds Practical Electro physiolo
4. cting the input signal The inputs can be used in single ended or differential configuration In differential configuration the signal which is connected to IN is subtracted from the signal that is connected to IN If only one input is used single ended configuration the other should be grounded to avoid coupling of noise 10 11 input coupling switches Pu e The position of the input coupling switch decides how the input signal is ld IN 0 li YA LaS di coupled AC the input signal is AC coupled with a corner frequency of 0 1 Hz DC the input signal is DC coupled O the input signal is grounded version 1 8 page 9 DPA 2FX User Manual 13 GAIN ZERO ADJ trimpots ZANE Trimpots to compensate for amplifier offsets If the baseline is not zero even if the input is grounded this offset can be compensated independently for the two GAIN stages Compensation procedure I Set the input coupling switches to zero to ground the inputs and set the HIGH PASS FILTER to 400 Hz LJ Set the GAIN range switch to 1 the GAIN factor switch to one and adjust the baseline to zero using the left trimpot Y Switch the GAIN factor switch to all positions and readjust to zero if necessary I Set the GAIN range switch to 10 If an offset is present compensate it using the right trimpot LJ Set the GAIN range switch to 100 and readjust the baseline if necessary 4 Literature L Barmashenko G Eysel U T amp Mittmann T 200
5. e zero position 1f one of the inputs is not used 3 3 Signal Flow Diagram The signal is passed through the DPA 2FX as shown below Zz ij AC 0 DC _ Selection E a Differential a Offset Highpass wo sues IN ac o pc a Input Compensation Filter gt Gain Stages Filter gt p Selection version 1 8 page 6 DPA 2FX User Manual 3 4 Description of the Front Panel and Operation O DPA 2FX npi O OFFSET ZERO ADJ a HIGHPASS Hz LOWPASS Hz 500 900 1 3k 600 700 1 5k 800 500 1 7k 1 0k 300 3 0k 3 0k 100 10k 10k 30 20k O ovER O over 6 O 0 IN IN OUT O O Figure 4 DPA 2FX front panel view version 1 8 page 7 DPA 2FX User Manual In the following description of the front panel elements each element has a number that is related to that in Figure 4 The number is followed by the name in uppercase letters written on the front panel and the type of the element in lowercase letters Then a short description of the element is given Signal OFFSET unit The OFFSET unit consists of 1 OFFSET range switch and 2 OFFSET potentiometer 1 OFFSET range switch 3 position switch for selecting the OFFSET range 0 1 V 1 V or 10 V 2 OFFSET potentiometer 10 turn potentiometer for compensating for a DC OFFSET Note The overall OFFSET compensation is the product of the reading o
6. f both controls Important Position 5 of the OFFSET potentiometer corresponds to O mV offset The offset compensation only works if the input coupling switch is set to DC If the HIGHPASS FILTER is used the OFFSET control is disabled GAIN unit The GAIN unit consists of 3 GAIN range switch and GAIN Mm 4 GAIN factors switch 3 GAIN range switch 3 position rotary switch for selecting the GAIN range x1 x10 or x100 4 GAIN factor switch 7 position rotary switch for selecting the GAIN factor x1 x2 x5 x10 x20 x50 or x 100 Note The overall GAIN is the product of the reading of both controls version 1 8 page 8 DPA 2FX User Manual Filter unit HIGHPASS Hz R LOWPASS Hz ea The filter unit consists of 5 LOWPASS filter and 12 HIGHPASS filter 30 Y 300 3 100 te 10K QFE me Me 10 Ol A 20 5 LOWPASS filter Single pole LOWPASS filter with 6 dB octave Corner frequencies 30 100 300 500 700 900 1 3k 1 5k 1 7k 3k 10k 20k Hz 12 HIGHPASS filter Single pole HIGHPASS filter with 6 dB octave Corner frequencies 3 30 100 300 400 500 600 800 1k 3k 10k Hz In OFF position the filter is disabled Note By combining HIGH and LOWPASS filters a filter with bandpass characteristic can be implemented easily 6 OVER OVER LEDs LEDs indicating that the DPA 2FX exceeds it s linear range 10 V 7 OUT connector BNC connectors for conne
7. gical Methods Wiley Liss New York LI Ogden D ed 1992 Microelectrode Techniques The Plymouth Workshop Handbook Second Edition The Company of Biologists Ltd Cambridge I Seidenbecher T and H C Pape 2001 Contribution of intralaminar thalamic nuclei to spike and wave discharges during spontaneous seizures in a genetic rat model of absence epilepsy European Journal of Neuroscience Vol 13 1537 1546 I Windhorst U and H Johansson eds 1999 Modern Techniques in Neuroscience Research Springer Berlin Heidelberg New York version 1 8 page 10 5 Technical Data DPA 2FX Input range Input impedance Input capacitance AC coupling at input OFFSET compensation HIGHPASS Filter LOWPASS Filter GAIN GAIN range Overload LEDs Output Size EPMS 07 SYSTEM Power requirements Dimensions version 1 8 DPA 2FX User Manual 12 V protected up to 15 V 10 MQ related to ground 30 pF corner frequency of 0 1 Hz Gf AC 0 DC switch at BNC connector is switched to AC set by ten turn potentiometer range 0 1 1 V or 10 V set by toggle switch single pole attenuation 6 dB octave corner frequencies Hz 3 30 100 300 400 500 600 800 1k 3k 10k single pole attenuation 6 dB octave corner frequencies Hz 30 100 300 500 700 900 1 3k 1 5k 1 7k 3k 10k 20k rotary switch 1 2 5 10 20 50 100 selectable with rotary switch x1 x10 x100 starting to light up a
8. ing system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is designed for use in scientific laboratories and must be operated only by trained staff General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrostatic discharge can be av
9. oided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 e after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals version 1 8 page 3 DPA 2FX User Manual 2 EPMS 07 Modular Plug In System 2 1 General System Description Operation The npi EPMS 07 is a modular system for processing of bioelectrical signals in electrophysiology The system is housed in a 19 rackmount cabinet 3U has room for up to 7 plug in units The plug in units are connected to power by a bus at the rear panel The plug in units must be kept in position by four screws M 2 5 x 10 The screws are important not only for mechanical stability but also for proper electrical connection to the system housing Free area must be protected with covers 2 2 EPMS 07 Housing The following items are shipped with the EPMS 07 housing Y EPMS 07 cabinet with built in power supply Y Mains cord Y Fuse2A 1l A slow Fron
10. t 10 V range 12 V into 1 KO 1 V into 50 Q front panel 12 HP 60 6 mm x 3U 128 5 mm 7 175 mm deep 115 230 V AC 60 50 Hz fuse 2 A 1 A slow 45 60 W dependent on the modules plugged in 19 rackmount cabinet 3U high 1U 1 32 44 45 mm page 11
11. t covers In order to avoid induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the housing 2 3 EPMS E 07 Housing The following items are shipped with the EPMS E 07 housing EPMS E 07 cabinet External Power supply PWR 03D Power cord PWR 03D to EPMS E 07 Mains chord Fuse 1 6 A 0 8 A slow Front covers SSSSSS The EPMS E 07 housing is designed for low noise operation especially for extracellular and multi channel amplifiers with plugged in filters It operates with an external power supply to minimize distortions of the signals caused by the power supply 2 4 PWR 03D The external power supply PWR 03D is capable of driving up to 3 EPMS E housings Each housing is connected by a 6 pole cable from the one of the three connectors on the front panel of the PWR 03D to the rear panel of the respective EPMS E housing see Figure 1 Figure 3 A POWER LED indicates that the PWR 03D is powered on see Figure 1 Power switch voltage selector and fuse are located at the rear panel see Figure 2 Note The chassis of the PWR 03D is connected to protective earth and it provides protective earth to the EPMS E housing if connected version 1 8 page 4 DPA 2FX User Manual PWR DID DRAN A Curr b AMA a Figure 1 PWR 03D front panel view Figure 2 PWR 03D rear panel view Note This power supply is intended to be used with npi EPMS E systems only 2 5 System Gro
12. unding EPMS 07 The 19 cabinet is grounded by the power cable through the ground pin of the mains connector protective earth In order to avoid ground loops the internal ground is isolated from the protective earth The internal ground is used on the BNC connectors or GROUND plugs of the modules that are inserted into the EPMS 07 housing The internal ground and mains ground protective earth can be connected by a wire using the ground plugs on the rear panel of the instrument It is not possible to predict whether measurements will be less or more noisy with the internal ground and mains ground connected We recommend that you try both arrangements to determine the best configuration a y amp EPMS E 07 i The 19 cabinet is connected to the PROTECTIVE EARTH connector at the rear panel The chassis is linked to protective earth only if the PWR 03D is connected It can be connected also to the SYSTEM GROUND SIGNAL GROUND on the rear panel of the instrument see Figure 3 Important Always adhere to the appropriate safety measures Figure 3 Rear panel connectors of the EPMS E 07 2 6 Technical Data 19 rackmount cabinet for up to 7 plug in units Dimensions 3U high 1U 1 3 4 44 45 mm 254 mm deep EPMS 07 Power supply 115 230 V AC 60 50 Hz fuse 2 A 1 A slow 45 60 W EPMS E 07 External power supply for EPMS E 115 230 V AC 60 50 Hz fuse 1 6 0 8 A slow Dimensions of External power supply W x D x
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