1. - Fisher UK Extranet
Contents
1. Please use always the nullmodem cable delivered with the osmometer If transmitting is impossible first check the cable and the settings of PC operating sytem and osmometer System requirements Free COM port MS Windows 3 1 or above and MS DOS 5 0 or above 9 Printer DR15 Type DPN 2333 Microprinter Manufacturer Datamega Please read and follow the enclosed user manual german english carefully For transmitting data from the osmometer to the printer use the enclosed cable DKA 205 If another power supply is necessary observe carefully the correct polarity and electrical values that are mentioned in the user manual Otherwi se the operation is incorrect or printer may be damaged When switching on the osmometer with the printer an introduction is printed and indicates the correct function If transmitting to printer DR 15 is impossible first check the cable and the settings of the osmometer see page 7 Printing example The introduction is printed after swit chingonand indicates the correct function of the printer The measuring result will be printed with identification ID number date and time of measuring A calibration in example CAL 0 will be printed with date time number and the average of calibration The measuring result will be printed with identification ID number date and time Me technik Usmometerer inter Tur i5 U1 8 25 81 2885 Result Sz most In 123456789812345672. 25 01 85 88 153 Cal 8 25 801 85 88 85 Ho Bi 82 mozm ijl a3verase SS B mosm EF tr Result 344 mozm Ib 3580 25 H1 85 88 05 of measuring Changing of ribbon cassette Please follow the instructions in the printer user manual Order number of ribbon cassette Epson ERC 22 or ERC 09 Loading paper Please follow the instructions in the printer user manual Outlines of paper roll Diameter 30 80 mm width 57 5 mm thickness 0 07 mm Cleaning Clean the housing with a soft tissue moistened with a little bit of water 10 Codereader Scanner SC15 Type FBC 3860 FuzzyScan manufacturer Cino Group Please follow the instructions in the scanner user manual carefully For transmitting data from the codereader to the osmometer use the enclosed cable The power supply of the Scanner is provided by the osmometer via the interface D sub plug with 5VDC 0 5A maximum The connection of pins is described on page 7 When switching on the osmometer with the scanner a beep from the scanner indicates the correct function If transmitting from scanner SC 15 is impossible or incorrect first check the cable and the settings of the osmometer see page 7 Configuration of codereader cognises the most ll Factory Default Setting current types of barcodes automa tically while scan Il II RS 232 Quick Set Command ning The interface parameters are If anew scanner of u
3. Clock battery The lifetime of the inserted lithium battery CR 2032 is more than 5 years If time and date are lost after switching off the osmometer the battery is empty Toreplaceit switch off the osmometer and wait for the display to go out Then turn off the lateral screws lift the cover upwards and replace battery After wards execute a date and time setting Usage Only for indoor use please keep dry Use only by following these operation instructions Location Only on a hard base table no sunshine keep away from radiators Keep the rear blow out of the fan and the bottom holes clear Operating temperature 0 C to 435 C maximum 80 relative humidity Storage temperature 20 C to 65 C Measuring range Measuring range 0 2500 mosm All higher concentrated samples must be diluted Reproducibility for solutions with low viscosity 0 5 in case of 50 ul sample volume 1 Laboratory instructions cleaning Observe laboratory instructions for handling infectious body fluids Clean the instrument by wiping off with a soft tissue moistened with a little bit of water with disinfectant It is prohibited to use sprays for cleaning The thermistor has to be dried with a soft tissue immediately after removing the sample tube Sample residues in the needle groove must be cleaned from time to time It is recommended to clean the needle cooling aperture daily or weekly depending on the number of measurements For det
4. 9 5 Puta new needle into the needle hole of the measuring head 6 Lift the right slider 7 by hand and shift the needle lever 8 to the measuring head 7 Insert the needle shaft into the slit under the fixing nut and fix it with the handscrew 9 slight bending 8 Putbackthe needle into its cooling aperture Attention The needle must go down slightly to its lowest position _ in the cooling aperture as well as in the sample tube Possibly an adjusting is needed Q N straight part 13 Maintenance Maintenance should be carried out only by qualified personnel The Osmo meter does not require fixed maintenance intervals 14 Technical details security Power supply protective class spare fuses Power supply AC 50 60 cps For supply voltage see label on the rear panel of the osmometer Power consumption Operating about 45 VA standby about 25 VA This equipment is according to protective class I and requires connection to protective earth for safety reasons Spare fuses Use only fuses in accordance with IEC 127 The Osmometer is delivered with two spare fuses T 400 mA 100 115 Volts T 630 mA and two spare fuses T 6 3 A which are fixed at the inner side of the rear panel Access can be gained by removing the yellow cover The instrument must be disconnected from the supply mains before the cover is removed Turn off lateral screws lift the cover upwards The osmometer is CE marked 11
5. gives a short tone The display now shows the re sult and print it if a printer op o supercooling Nu EE tion is connected The last 100 U measurements are stored in the fmi Erin ame memory Later output on dis Typical cooling curves of water and play printer or PC interface is aqueous solutions possible Because of the linear correlation between osmolality and freezing point the measurement values are displayed in milliosmol kg H O mosm and not C 3 Operation 100 yl sample tubes We recommend Sarstedt reagent tubes which we use when adjusting the thermistor before delivering the Osmometer Order No Sarstedt 72 690 The instrument is adjusted at the factory for a sample volume of 100 pl and the tip of the thermistor has a j distance of 2 mm to the bottom of the sample tube If different sample tubes are used the position of the thermistor must be checked and readjusted if neces sary Westrongly advise against the use of asymmetric sample tubes or such fitting only loosely into the cooling aperture This causes poor reproducibility of measurements 2 The instrument must be calibrated prior to commencing measurements Measurement and calibration must always be carried out using the same thermistor positions and the same sample volumes The Osmometer is fitted with a clear text display The advice and messages explain the operation Please follow the
6. the same type is Record Suffix connected or the The scanner re N C gt settings are lost so read the opposite 5 III 6 for EOT 04h barcodes in the numerous order to restore the factory 6 Ill MI End Exit settings Cleaning Clean the housing with a soft tissue moistened with a little bit of water 11 Thermistor replacement Switch off the osmometer and wait for the display to go out The thermistor is provided with a suitable precision resistor and a mini plug Access can be gained to the mini connectors inside the measuring head to which the thermistor is connected by removing the black measuring head cover 6 The thermistor must be fixed in the measuring head in the right position with the plastic screw The distance of 2 mm to the bottom of the sample tube should be adjusted exactly see also page 2 Subsequently a new calibration with distilled water and then one with 300 mosm standard and if selected with 900 mosm standard is required See pages 3 and 4 Every osmometer is supplied with one spare thermistor 10 12 Exchange of the freezing needle Only the original freezing needles should be used The Osmometer is supplied with a spare freezing needle 1 Switch off the osmometer and wait for the display to go out 2 Putan empty sample tube 4 at the measuring head 6 N Push the measuring head down IN 4 Remove the old needle 11 by loosing the handscrew
7. Flow control DTR DSR DTR DSR DSR DTR DSR DTR DCD DCD DCD DCD The interfaces are only for dataflow from Osmometer to printer computer or codereader For using with printer it is useful to connect the flow control lines like described above For using with a personal computer it is possible to run without flow control The RS 232 data are ANSI the printer data are ASCII both with CR LF for every printing line and an EOT sign for end of transmission 8 Data transmitting to a personal computer To receive data with a personal computer from the RS 232 interface of the osmometer connect it with the delivered nullmodem cabel to a free serial port COMI COM4 of the PC Then install the delivered terminal program Com15V10 To do so start the file Setup exe from the delivered data carrier out of the windows explorer and follow the on screen instructions The setup program creates on the specified drive a directory Com15V10 containing the program files Now start the file Com15V10 exe out of the windows explorer The terminal program checks all COM ports Select the desired port by pressing F3 The selected port appears down in the status line of the window Select F1 for advices to use the program or help Important The received data is stored as a text file txt and an excel file xls The next start of the terminal program overwrites these files Please copy or move these files if you still need them
8. France Belgique Belgi Fisher t l 0388671414 tel 056260260 Bioblock Scientific 0388671168 fex 056260270 email infos bioblock fr email belgium bioblock com Parc d innovation BP 50111 F67403 illkirch cedex www bioblock com www bioblock be Mode d emploi Espana tfno 915159234 fax 91 515 92 35 email ventas bioblock com www es fishersci com E15 Tl 1 2005 Micro Osmometer Type 15 OSMOMETER Operating instructions L ser Messtechnik D 13589 Berlin Kaiserstra e 24 Phone 49 0 30 375 10 800 Fax 49 0 30 375 10 809 Contents 1 on o d 10 11 12 13 14 15 16 17 18 19 20 GMS ral mE 1 Definition of peltier effect uuunnsnnnerssnnnenennnensnnnen nennen nennen ernennen 1 Pelir Cooling e tente 1 Measuring principle een 1 PSN ALON sesso 2 Calibration kisser contin 3 Calibration with distilled water Zero 3 Calibration with standard 300 MOSM sse 3 Calibration with standard 900 MOSM sseeeeee 4 General information for calibration 4 Measurement Measuring values storing Special advices Configuration of the osmometer Language System Interfaces Pin connection and parameters ssse Data transmission to a pers
9. ails see page 6 15 Explanation recycling Specialexplanationto the operating personnelis not necessary because of the clear text display We recommend the observing of this operating instructions Therecycling of an old Osmometer should only be carried out by a recycling company Rev January 31 2005 12 16 Clear text display Display Status Display Result Ready 290 Display Status of measurement Display Advices error messages Status Measurement o k Advices and messages tAdvices and messages 17 Menu structure Osmometer menu Ready Value output Calibration Service Language Calibration 0 F Calibration 300 Calibration 900 Display cal value 0 Display cal val 300 Display cal val 900 Clear calibr memory Deactivate Cal 900 deutsch r english F francais espanol L portugues svenska Value display Print 10 values Print 20 values Print 50 values Print 100 values Thawing needle hole Cleaning needle hole System _ Date Time setup FRS 232 interface Baudrate Frey Flow control r Printer interface Baudrate L Pariy Flow control Codereader interface Baudrate L Pariy Flow control Activate deactivate codereader Toggle 13 18 Troubleshooting Message possible reason Solution Thermistor error Swit
10. ce Error freezing too early Dust in the sample tube Old or rough sample tube Thermistor scratched Use new sample tube Use new sample tube Change thermistor Error No value within the search time There is no exact freezing point reached within the search time ie centrifuge sample and separate solid parts 14 19 Operating elements connectors OND 10 11 12 13 14 15 16 17 oe ON to waste bottle Ready NU 1 298 DOOR Measurement o k u 4 E Enter 5 2 2 a 6 3 9 10 OSMOMETER 11 Display Osmometer ready Keyboard Power switch Sample tube Keys C 12 Menu and Enter mor OS 8 Measuring head cover ee Needleslider right Cay un ca Lever for freezing needle K 13 N Freezing needle N 14 fixing screw N 15 Needle stop Freezing needle 2 Analogue output option E e 16 Codereader socket Printer socket RS 232 socket S CEE mains plug ER Hose connection Qe 17 15 20 EU Conformity EU Declaration of Conformity Manufacturer Axel L ser Me technik 13589 Berlin Rauchstra e 2 Identification of the product name type variant Osmometer Type 15 The product identified above complies with the requirements of the following EEC dir
11. ch Thermistor short circuit Replace thermistor Switch off and contact your local service off and check the Bridge faulty Contact your local service thermistor Thermistor cut Switch Thermistor broken Replace thermistor off and check the Bridge faulty Contact your local service thermistor Error in the reference Bridge faulty Contact your local service Error with the needle drive Switch off and contact your local service Motor control faulty Contact your local service Error Needle movement is blocked Neddle blocked Motor does not run Motor control faulty Correct manually Contact your local service Contact your local service Codereader error Codereader activated but not connected Check cable or connection Error Undercooling is too slow Wrong cabel used see chapter 7 Room temperature too high or unit placed in the sun Peltier element faulty Unfitting sample tube or faulty Contact your local service Terminal or printer Faulty cable Change cable not ready or not connected check connection Output will be terminated Wrong parameters Correct it Use correct cable Place instrument in a cool and shady place Contact your local service Use recommended ones Error freezing the sample is impossible Sample too concentrated Sample not able to freeze Peltier element faulty Dilute sample Measurement impossible Contact your local servi
12. ectives Richtlinie 98 79 EG des europ ischen Parlamentes und des Rates vom 27 Oktober 1998 ber In Vitro Diagnostika Proven by meeting the following European harmonised Standards EN 61326 1 M rz 2002 Elektrische Betriebsmittel f r Leittechnik und Laboreinsatz EMV Anforderungen EN 61000 4 2 2001 EN 61000 4 3 2003 EN 61000 4 4 2002 EN 61000 4 5 2001 EN 61000 4 6 2001 EN 61000 4 7 2003 EN 61000 4 8 2001 EN 61000 4 11 2001 Attaching of CE Mark 2005 Location Date Berlin den 7 Januar 2005 Signature 16 Micro Osmometer Type 15 E Short form Operating instructions eH E Result L L ser Messtechnik D 13589 Berlin Kaiserstra e 24 l ser Phone 49 0 30 375 10 800 Fax 49 0 30 37510809 e5xa T
13. er and 12 RS 232 interface pin abbr in out OO O O function 67 8 9 DCD RXD TXD DTR SG DSR RTS CTS RI NI O1 amp ND n Data carrier detected connected to pin 4 and pin 6 Receive data Transmit data Data terminal ready connected to pin 6 Signal ground Data set ready connected to pin 4 Request to send Clear to send Ring indicator no connection Pin connection of 9 pin D SUB male plug for codereader scanner pin abbr in out function 1 O 2 RXD I 3 TXD O i DIR O 5 SG 6 DSR I 7 RIS O 8 CTS I 9 3 O 5 V power supply for codereader Receive data Transmit data Data terminal ready connected to pin 6 Signal ground Data set ready connected to pin 4 Request to send Clear to send 5 V connected to pin 1 Observe the same settings at the Osmometer and the connected equipment Parameters Baudrate selectable 8 data bits parity selectable 1 stop bit Flow control selectable Definition of XON XOFF XON 11h XOFF 13h ASCII charakters For the printer DR 15 are valid 9600 baud no parity Flow control XOn XOff If another printer is connected the corresponding settings are necessary For the scanner SC 15 are valid 9600 baud no parity no flow control Osmometer interface to printer computer or codereader RXD TXD RXD TXD TXD RXD TXD RXD RIS CTS with RTS CTS without CTS RTS Flow control CTS RTS
14. hen the osmometer repeats the needle movement up to three times If no crystallization starts the osmometer displays the message freezing the sample is impossible By lifting the measuring head the osmometer goes back to the status Ready The automatic thawing function avoids icing of the sample cooling aperture When it is very humid after prolonged use it is possible that a drop of pure thawing water comes out of the drain under the Osmometer which can be easy wiped off Storing measuring values The last 100 measurements are stored in the memory which can be displayed by selecting the item in the menu The output of the last 10 20 50 or 100 measuring values with sample number date and time to the connected printer DR 15 and the RS 232 interface is also possible The newest value appears in first position 6 Special advices Blocking the needle with frozen water is prevented under normal conditions by the automatic thawing function The thawing water from the needle cooling aperture is automatically sucked off and collected in the waste bottle Note This waste may contain infectious residues of samples Dispose expertly In the case case of very high humidity the needle can become blocked Manual thawing is recommendable selectable by menu A beep indicates the end of thawing The menu Iso allows the cleaning of the needle aperture Select the correspon ding item of the Service menu To fill in the cleaning fluid
15. ibration is comprehensible later Additionally thecalibration with number date and timeis given outatthe RS 232 interface and printer If a standard solution was used inadvertently the message Please measure with water will be displayed Remove sample tube carefully Remaining ice on the thermistor must be removed with care by means of sample tube Important Sampleresidues on the thermistor will falsify next measurement For that reason wipe thermistor each time with a soft tissue Calibration with standard 300 mosm 1 2 Fill sample tube with 100 ul 10 of 300 mosm standard to 5 Same steps as calibration zero see above 6 Changing values while freezing are displayed When the buzzer sounds and the display shows Measurement completed the value for 300 mosm is determined and stored in the memory Additionally the measuring value with number date and time is given out at RS 232 interface and printer option Raise the measuring head If the display does not show 300 mosm enter the menu Calibration and select Calibration 300 The Enter key mustbe pressed to setthe300 mosm value The message calibration o k is displayed and in the memory the sample number changes to Cal 300 So the calibration is comprehensible later Additional the calibration with number date and time is given out at RS 232 interface and printer If water or 900 mosm solution was used inadvertently the mes
16. is named after Jean Peltier 1785 1845 Peltier cooling By using Peltier elements small amounts of liquid can be cooled and frozen Peltier elements become warm on one and cool on the other side The cold side isin thermal contact with a metal block which contains two apertures one for the sample tube and another for the freezing needle The warm side is mounted onto a cooling surface The necessary air cooling is provided by a fan The Peltier element is charged with a current from a regulated power supply This is controlled by a thermistor in order to keep the temperature of the metal block constant 2 Measuring principle The freezing point of aqueous solutions is measured The freezing point depression in comparison to pure water is a direct measure of the osmotic concentration Pure water freezes at0 C an aqueous solution with an osmola lity of 10smol kg H O a freezes at 1 858 C The sample tube contai T 4 Wheatstone bridge cH ning the sample is placed onto the measuring head which inserts a thermistor Measuring head Sample tube into the sample The ther Freezing needle mistor is part of a Wheat Thermistor A I x stone bridge Nee Cooling units Two or three values are LI Ready 1 Peltier element 1298 mom stored electronically One Display Status f
17. leaned A difference of 1 or 1 mosm from the nominal value is tolerable Should the difference be considerable repeat the measurement with afresh sample filled into a new sample tube Recalibration may not be necessary Some examples are listed below in general sense also for 900 mosm Measurement H O 300 mosm Result 1 Sample 0005 0305 Thermistor was dirty with sample re sidues Zero adjustment was correct 2 Sample e 0304 A recalibration with the first sample 3 Sample 0000 0300 would be a mistake Measurement H O 300 mosm Result oo 1 Sample 0005 0305 In this case a recalibration is necessa 2 Sample 0005 0306 24 3 Sample 0005 0304 It is recommendable to use a NaCl solution 0 9 286 3 mosm as a control for plausibility 5 Measurement The measurements will be done in the same steps as described under calibra tion item 4 Use a fresh sample for every measurement filled into a new sample tube If itis necessary to measure the same sample a second time it must be stirred after thawing to avoid concentration gradients It may be advisable to measure a new pipetted sample In case of dust in the sample tube or if the same tube is used often and or not correct cleaned the sample freezes before reaching the initiation point of 6 2 C The message Freezing too early appears in the display High concentrated samples or those difficult to freeze may cause a delayed freezing T
18. m For calling the Osmometer menu Page 13 push the Menu key 5 Mark with Tandil keys item is inverted and select with the Enter key 5 If you want to go a step backwards push the Menu key 4 Calibration Calibration with distilled water Zero Switch power on and wait for ready display about three minutes Fill sample tube with 100 yl distilled water volume must be 10 Attach sample tube 4 to measuring head 6 Depress measuring head so that sample tube enters cooling aperture Enter sample number with keyboard 2 or codereader option Supercooling is reached in about 1 minute At 6 2 the freezing needle 11 is automatically taken out of the cooling aperture and inserted into the sample for about one second then it is returned to its original position Display Sample freezing Changing values while approaching the freezing plateau are displayed When the buzzer sounds and the display shows Measurement completed the value for water is determined and stored in the memory Additionally the measuring value with number date and time is given out at the RS 232 interface and printer option Raise the measuring head If the display does notshow 0 mosm enter the menu Calibration and select Calibration 0 The Enter key must be pressed to set the zero value The message calibration o k is displayed and in the memory the sample number changes to Cal 0 So the cal
19. onal computer 8 Printer DR QC den 8 Printing example sasirnane ETE eee tede oe 9 Changing ribbon cassette ssesssseeeeenee 9 Loading papain itr tente teorie erae Ere Oe xe pei abaR 9 CISANINOS ER E 9 Codereader Scanner SC 15 9 Configuration of codereader sene 10 GIS ANN Ges ETE 10 Thermistor replacement 10 Exchange of the freezing needle 2511 Maintenance 11 Technical details security 11 Power supply protective class spare fuses 3141 Clock battery sss s 12 WY SAGO c 12 Measuring range laboratory instructions cleaning 12 Explanation recycling seen 12 Clear text display essen 13 oe eI 13 TTOUDISSNOOtINQ e 14 Operating elements connectors folding page 15 EU conformity AR 16 Operating instructions Automatic Micro Osmometer Type 15 1 General Definition of peltier effect When an electric current flows across the junction between two different metals or semiconductors a quantity of heat proportional to the total electric chargecrossing thejunction is evolved or absorbed depending on the direction of the current This effect is due to the existence of an electromotive force at the junction and
20. only use the soft plastic funnel delivered with the osmometer to prevent any damage of the needle aperture Never use force or sharp or pointed tools For cleanig fluid use only water perhaps with a small amount of washing up liquid A buzzertone indicates the end of cleaning Depending on the number of measurements a daily cleaning is recommended necessary cleaning is at least one time a week 7 Configuration of the osmometer Language Several languages are selectable in the Language menu System Several settings are possible with the System menu Select the correspon ding item and follow the messages 1 Date and time setting 2 Configure RS 232 interface 3 Configure printer interface 4 Configure codereader scanner interface 5 Codereader activate deactivate Interfaces The osmometer has serial interfaces which are in accordance to the American standard EIA RS 232 C This and the German standard DIN 66020 describe the electrical properties Voltage 15 Volt maximum Signal voltage between 3 Volt and 3 Volt is not permitted because the logical level is defined as follows Mark Logical 1 for the voltage from 3 V to 12 V Space Logical 0 for the voltage from 3 V to 12 V For connection of the codereader the printer or a personal computer only use the cables delivered with the osmometer The connectors are on the rear panel of the osmometer Pin connection of 9 pin D SUB male plug for print
21. or the freezing point of Measurement o k g ok Heat sink distilled water and anoth er one or two for standard solutions Based on these values a microprocessor calculates all further measuring values They are displayed with necessary information on the text display The sample starts off at room temperature Using a pipette it is put into a sample tube which is placed onto the measuring head This will be slid down on its guide rods inserting the sample tube into the cone shaped cooling aperture Now the sample begins to be cooled The display will show decre asing values Once zero is reached increasing negative values will be display ed In clean sample tubes aqueous solutions are easily supercooled i e the sample does not freeze although the freezing point has been passed At a defined supercooling when the display shows 6 2 a cooled needle with ice crystals is inserted automatically into the sample to initiate the freezing The temperature will begin to rise until the freezing point is reached The point of inflection thus achieved is the value Temp temp Pure water produces a prolon FONS CREE plateau ged plateau whereas solutions T N have a turning point of only a few seconds duration The ap T proach to the plateau or the Freezing point depression turning point will be indicated and automatically recognized turning point mosm The final value will be stored aqueous solution and a buzzer
22. sage Please measure with 300 mosm will be displayed Fresh samples should be used for each measurement 7 and 8 as page 3 Calibration with standard 900 mosm 1 to 8 Same steps as calibration with standard 300 mosm 9 Cal 900 appears in the display now the measurements will be done with 3 point calibration The calibration with 900 mosm is only practical if the expected values are above 600 mosm This calibration point gives more exact measurements for higher concentrated solutions If all samples are in the range of about 300 mosm the 900 mosm calibration is unnecessary Select Deactivate Cal 900 from the calibration menu and press Enter A buzzer sounds and Cal 900 disappears from the display Now the measurements will be done with 2 point calibration General information for calibration Every calibration point consists of the average of max 10 calibrations since switching on the osmometer To display select the corresponding item in the menu After switching off the osmometer the calibration memory is erased only the averages remain in the memory for further measurements If a new average is requested i e in case of miscalibration the calibration memory is manually erasable by selecting the item in the menu The calibration is stable for a considerable time In general use contamination of the thermistor may cause the zero or standard calibration point to vary Before recalibrating the thermistor must be c
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