ValveBank Man 3-00 - AutoMate Scientific
Contents
1. Close CANCEL Il IG seo OC GO ARROWS change selection Menus ENTER makes selection CANCEL returns to last screen AutoMate Scientific Inc The ValveBank8 Calc 17 Manual Mode Manual mode allows user control of valves and TTL outputs for easy set up testing calibration and cleaning MANUAL MODE V ICL 2CL 3CL 4CL lt Status Bar see below for explanation Follow instructions printed on the ValveBank A low click from valves indicates opening or closing A hum from a valve indicates that it is holding open at the appropriate voltage Toggle between V alves and T TL Mode Look for a V or T in upper right corner Selects whether ALL Open Closed 9 and 0 commands affect valves or TTL outputs Leave Manual Mode Emergency ALL CLOSED Closes all valves and sets all TTL outputs to Low Toggle One at a time Mode on off When on an O appears in upper right corner and opening a valve will close all others for perfusion use CANCEL Manual Mode Special Features Menu ENTER see next page V alve or T TL ine ata ene Mode Mode for all on off on off see lt Lt Rt see above This T means Arrows gt above u Timed Manual Inverted number is Mode is in use HT Manual Mode O a Master channel see next page 1CL 2CL 3CL CLI see next page HVT T TTLstate H igh L ow L v7 _ Valve state O pen C losed2. 0 Additional manual commands while a program is executing Ist press Pauses any running program All valves and TIL s remain open or closed as they were when paused 2nd press Closes all valves returns TTL s to Low Returns to Main Menu ENTER Buzzer OFF Resumes paused program When the program is completed the display will be left showing the final time including fractions of seconds 01 T 07 36 11 84 Program Finished CANCEL Press lt Cancel gt to return to Main Menu All valves Closed unless run from Manual Mode and completion time shown Single Repeat Running Microinjecting See Repeat Run in Configuration for rapid repeating Press key lt 1 gt twice after a program ends to run it again useful for microinjecting repeatedly Manual Mode Special Features Special Manual Mode Features One at a Time Master and Timed Modes are IGNORED by VALVE ON and OFF commands but still work for manually toggling valves open and closed See pg 19 for descriptions They must be turned on and off in Manual Mode since lt Enter gt will not access the Special Features Menu while running a program 31 Serial Mode Serial mode is used to transfer programs back and forth between the ValveBank and a computer running the AutoMate graphic program ming package EasyCode Programs can be entered and edited on a PC or Macintosh then downloaded into the ValveBank s battery memory for running further editing or
3. 16 Manual Mode 18 Manual Mode Special Features MEt 19 Edit Foote Mod en nennen 21 Sv a PrO TAT arena 22 Next Time Command Prediebon seisein eii iei 23 COMMAS reiner 25 LOB ana 26 EOOP DEU ee en rite rr rrer fects rrr rere rrr ry 27 TELSTAR Oman ee ee 27 Peene cl I en ee ee 29 The ValveBank is intended for research use only The ValveBank line is dedicated to the memory of Victor A Rivas R n Mode nennen nee 30 Networking multiple ValveBaiks sn 30 Kunming Programs emeente adeldom 30 Single Repeat Running Micr injectins ann 31 Manual Mode Spel Ren USS nee 31 Serial Mode i ssctssesssssssanvsasesssvasassnssiinssesassssessesdussnesesonssonveasennsnssaasasnsevesesss 32 File MiG Ge ssasisas sssesscetsisssasnesievacaiveatennsiviads sveiecbosivisienstasestiausseiasteataasiavasedss 33 lear Al CO ennen ont 33 Computer Interi acin sanken neuen 34 ED ee See rei 34 Fecdpack Io rear ED een 35 Slave Mode Computer EWS een 35 TEC BOTES sense on Teeenrre tT Terr ve tr very ny rrer errr yer 36 Dead Yol m rispari O ee 36 Small bots Tero TUS nenne 36 BCN OM ee een 37 Ove IS CSCLY OIG amendement entend 37 Autor nme SLE s 37 Be VO era rein 38 DEREK PIC Val VES see en ee ee 39 Valve CODE ee een 39 Valve Reims amp Replace a Vale en ana a 40 Leaks amp Replacing valve MNOS een 41 EPROM Installation nsruchons ea 42 Configuration Technical NoteSs sesesesesosesesesesesoscscsosesesssesesseessess 43 Networking multiple va
4. Assemble ringstand 2 Attach valve unit 3 Attach reservoir bracket s 4 Insert reservoirs 5 Attach provided tubing to upper valve barb cut as desired but see note above first 6 Connect tubing between bottom valve barbs and manifold 7 Connect flow regulator tubing to manifold outflow and your perfusion chamber downsizing if necessary 8 Plug numbered valve cables into ValveBank ports Calibrate flow rates by running one line into a graduated cylinder for a fixed period of time Standard Teflon Perfusion System without pressure ValveBank Green clamps close to mani fold vacuum trap Unused lines should be replaced with plugs on manifold or temporarily clamped off just above manifold to avoid back bleeding into empty lines Priming one line at a time reduces bubbles Connect your vacuum trap line directly to the outflow of the manifold for running cleaning solutions and drying your lines quickly Determine dead volume from the manifold to sample by measuring time and volume required to flush a colored liquid with a clear one Flow rate adjusted by relative height of reservoirs and included flow regulator Always calibrate flow rates regulator values are relative see below Leave tubing between drippers and valves long enough to raise and lower reservoirs 1 Assemble ringstand 2 Attach reservoir bracket 3 Attach valve unit 4 Insert rese
5. Channel 1 through 4 or 8 Status Bar 18 Manual Mode Special Features Menu ENTER Makes selection CANCEL Returns to Manual Mode Jumps to next screen Run a program Yes gt No Answer Yes to run a user program from Manual Mode The current valve and TTL state is preserved may be changed later by your program Control is returned to Manual Mode after the program is completed or aborted again with opened valves and TTL s remain ing open Useful for running a protocol after establishing a stable baseline or pre programmed wash outs and line priming Edit master ch Choose a master Yes gt No 1 2205 4 Using a Master channel insures at least one valve is always open When you close the last open valve the Master valve will open Master channel does NOT open after pressing 0 for All Closed gt gt T 00 00 20 00 Enter Duration a Er When Timed Manual Mode is Y turned on any valves designated will remain open only for pre set amount of time then close again opening the Master channel above if also being used A Master channel may also be timed but if it is the only valve open it will not close Timed channels will NOT turn off after pressing 9 All Open Use Timed Open Yes gt No One at a Time Mode insures that no more than one valve is open at a time Opening any valve closes all others Pressing 9 for All Open ignores One at a Time Mode 19 Examples Ch 1 Valve Open TTL High Man
6. accordingly Example 04 T 00 00 10 00 When you finish entering the LOOP Ch1 LOOP STRT 12 END command shown the Valve Bank determines when the loop will 09 T 00 00 20 00 be completed and enters this time on Ch1 LOOP END the next command line 10 T 00 02 10 00 Remember that the first pass Chi VALVE ON through the loop counts as its first iteration 23 Write a new program which opens valve one for five minutes then valve three for six hours follow the underline cursor in the examples Progressive display Select Channel Channel 1 01 T 00 00 00 08 Chi END OF LIST 01 T 00 00 00 00 Ch1 END OF LIST 01 T 00 00 00 00 Chi VALVE ON 02 T 00 00 00 00 Chi END OF LIST 02 T 00 00 00 00 Chi END OF LIST 02 T 00 05 00 00 Chi VALVE OFF Select Channel Channel 2 01 T 00 00 00 00 Ch2 END OF LIST 01 T 00 05 00 00 Ch2 VALVE ON 02 T 06 05 00 00 Ch2 VALVE OFF Keys Pressed Pri NTE 7 ANCE NTER rm gt N Y p WOWWWHWWHWW ANCE ANCE SISE Save Program gt YES NO No 4 Several Result see next screen Warning Start new program Begin with channel 1 Move right and down to edit first command Scroll through commands to Valve On Next line Note Move to minutes digit VALVE ON Enter time 5 minutes VALVE OFF then move down TTL ON Valve Off auto selected TTL OFF Done now lt Cancel gt to program other channel BUZZER ON BUZZER OFF Select next c
7. cable with null modem adapter with the following simple wiring 1 1 ground 2 3 2 45 Troubleshooting Should the ValveBank screen and power LED fail to light while power is turned on check your power connections source and on off switch Disconnect all valves to check for a short Finally if all else fails unplug the ValveBank remove the six screws on its bottom and check the fuse on the internal circuit board Only replace the fuse with a standard not slow blow 2 5 Amp fuse One extra fuse is included on the circuit board in clips labeled Spare Also see the Techniques chapter for more troubleshooting information on problem valves and leaks The ValveBank is designed to drive 12V DC 2 way solenoid valves Its driver circuitry is overload and short circuit protected 46 EasyCode Macintosh amp PC Software Program your valve and pump on off sequences with a Macintosh or IBM using graphic timebars Save and load unlimited programs to disk print out program listings then download your programs into the ValveBank s memory in one simple step Once downloaded the program remains in the ValveBank which can be disconnected from the computer See Serial Mode on p 32 for program transfer information rr Tre ave lh wnal Ier Chancel ee 111 me oo aa foo aa Diack alen End Tima oo 25 45 zo aa J lep SS TIL In Jpilan Chen cl wiles Ea ANTEC hd rr r u
8. gt return to previous screen lt Lt Rt Arrows gt change selection Save Program YES NO 22 If the program number already contains a program the user will be asked for confirmation to overwrite lt Enter gt to make selection lt Cancel gt returns to previous screen lt Lt Rt Arrows gt change selection Overwrite 0K ES NO After a successful save the ValveBank displays a confirmation message and returns the user to the main menu Next Time Command Prediction In order to speed your programming the ValveBank automatically predicts the next time and command you will use and fills them in on the next programming line for you For the time line it uses the time that the previous command will finish Then it predicts the compliment of the previous command Valve Off after Valve On Buzzer Off after Buzzer On etc You can always change the time and command that it guesses it just makes programming easier The ValveBank only predicts after you ve changed the previous line Total Loop Time Calculation Loop structures are explained in the next chapter on Commands As you enter a program with a loop and finish entering its LOOP END command with a time the ValveBank calculates the total length of the loop multiplied by the number of iterations you have entered and enters this finishing time on the next programming line If you change the loop start loop end or iterations later you may need to revise subsequent command times
9. the cost of the product All other warranties including but not limited to warranties for fitness or merchantability for a particular purpose are expressly excluded No verbal changes to this policy will be authorized AutoMate Scientific Inc manufacturers and sells goods in accordance with U S federal state and local laws but assumes no responsibility or liability for laws regulations duties or taxes imposed by any foreign country All terms and conditions hereof shall be governed by the laws of the State of California 53
10. u 2 lu o liy fi tigitiii e I uy eega vlan uu eo MAN For Single cell or Whole cell Superfusion Multi barrel Perfusion Pencil 8 micro bore tubes into 1 outflow e Single cell and patch delivery e Rapid solution change e Micro liter dead volume e 360 250 amp 100 um removable tips System Set up Diagrams Perfusion System ValveBank vacuum trap Unused lines should be replaced with plugs on manifold or temporarily clamped off just above manifold to avoid back bleeding into empty lines Priming one line at a time reduces bubbles Connect your vacuum trap line directly to the outflow of the manifold for running cleaning solutions and drying your lines quickly Determine dead volume from the manifold to sample by measuring time and volume required to flush a colored liquid with a clear one 8 Instructions on this page for Lure lock fittings Pressurized systems or Pinch valve systems Regular hose barb Teflon perfusion systems please see the next page Lure lok systems should also see photo on page 6 Find additional photos and diagrams of the pressure system on pages 7 and 13 Flow rate adjusted by relative height of reservoirs and included flow regulator Always calibrate flow rates regulator values are relative see below Leave tubing between reservoirs and valves long enough to raise and lower reservoirs 1
11. Channel 4 CH4 VALVE ON 02 T 00 12 20 00 CH4 VALVE OFF 01 T 00 12 20 00 CH3 LOOP STRT 03 Prepare to repeat drains 03 T 00 12 20 00 Channel 4 CH4 LOOP STRT 03 02 T 00 12 20 00 CH3 VALVE ON 03 T 00 12 50 00 CH3 VALVE OFF 04 T 00 27 00 00 CH4 VALVE ON 05 T 00 27 20 00 CH4 VALVE OFF 04 T 00 27 20 00 CH3 LOOP END 06 T 00 27 20 00 CH4 LOOP END Prepare to repeat washes Channel 3 Fill tray with wash solution Wash for 15 minutes Drain Repeat 3 times in both channels 50 Safety Instructions The following instructions pertain to the risk of fire electric shock or bodily injury Please read all of these instructions carefully 1 Follow all the instructions and warnings marked on this product or included in this manual 2 Do not use this product on an unstable cart stand or table This product may fall causing serious damage to the product 3 Slots and openings in the cabinet and the back are provided for ventilation To ensure the reliable operation of your product and to protect it from overheating these openings must not be blocked or covered Do not use this product on a bed sofa rug or other similar surfaces This product should never be placed near or over a radiator or heat register This product should not be placed in a built in installation unless proper ventilation is provided 4 Never push objects of any kind into the product through the cabinet openings as they may touch da
12. Iruj per In Arnage H Trigger Time oo 05 oo aa Chromatography a Bank 1 3 Channel 1 B iks Lan Bl Time OO DD OD Start Loop WORST DD 000 Valve On DD 1 00 O0 Valve Off DAD A0 End Loop e ee Supports up to 16 channels 4 networked ValveBank4 s or 2 ValveBank8 s Channel 2 e Open multiple experiment windows u un e Full zoom and reduce capability lets you DOD 00 Start Loop g g 00 01 00 00 Valve On make fine adjustments or see your entire 00 02 00 00 Valve Off 00 03 32 40 TTL On experiment 00 04 00 00 TTL Off 00040060 End Loop ii e Double click to enter start and stop times OODANUD End Or duets accurate to 0 01 seconds from the keyboard e Cut and paste timebars or entire channels Channel 3 N Keen even between several experiments on Sa Supports all ValveBank commands including ENEN alive OT EEF a 00 03 00 60 Valve Off multi channel looping and interface triggering e Receive programs from the ValveBank to print out or modify e Review ValveBank commands as text listing on screen or printed e Requires min Mac Plus 1 meg RAM system 6 or greater or PC running Windows 3 1 47 Serial cable and manual included Sample Programs Chromatography lon exchange Perfusion 0 1M NaCl pH 7 0 01 T 00 00 00 00 Salt 1 for 1 hour CH1 VALVE ON Saline Buffer 1 for 5 minutes 01 T 00 00 00 00 Channel 1 OQ Channel 1 CH1 VALVE ON 02 T 01 00 00 00 CH1 VALVE OFF 02 T 00 05 00 00 CH1
13. Microliter fine delivery e Small diameter pipette delivery 5 File Edit Time sec 10 sec Display Experiment Program Simple Test Bank 1 Channel 1 er Time _ event The package can be added to NaOH 0 1 M 00 00 00 00 Valve On any new or existing perfusion Buffer 1 OOOO 30 Valve Off e f A M f 00 00 03 00 TTL On rig from AutoMate any Channel 2 ee third party manufacturer or ODO 10 TTL Off Sample 1 eet ae even homemade Connect to Cr 10 0 1 End Of List house air tank or compres sor 30 to 100 psi Precision regulator delivers zero to 10 Channel 3 NaOH 1 6 M Buffer 2 Channel 2 Time Event OOC OD Loop Start 10 si 00 00 01 000 TTL On psi Channel 4 valve 00 00 01 550 TTL Off EN ttl 00 00 02 40 Valve On ee Rn 00 00 04 650 Walve Off p P 00 00 07 440 TTL On I 00 00 07 90 TTL Off __Begin Display From B ol 000 onen AutoMate also offers valves with luer lock fittings for direct connection of syringes This method eliminates wasted solution in tubing between the reservoirs and valves Small 2 way stopcocks are included when this option is ordered e RRS uli o S _ _ _ ut O0 00 10 01 o ee ens uuhyhuduuhuhudea luubi lielpa 2 adenentatedetovsten busenbreduduul g e N i End Of List Hill 5 sn 5 wg 8 ili B
14. ND OF LIST is entered accidentally BLANK Loops LOOP STRT X01 LOOP END 06 T 00 12 04 00 Chi LOOP STRT 01 06 T 00 12 04 00 Chi LOOP STRT Q1 06 T 00 12 04 00 GERO Oessn nen ENTE 26 Automatically inserted by the ValveBank after the last user command or can be entered by the user to erase an existing channel Represents the last line of a channel s programming A running program finishes when it reaches the END OF LIST in all four channels User can never scroll down past a END OF LIST command therefore any commands below an inserted END OF LIST will be inaccessible amp lost 1 Press lt Cancel gt 2 Do not Save Program 3 Return to Select Program screen 4 Re load the last saved version of your program Note This will erase any changes made since the last time the program was saved but will recover any programming the END OF LIST erased Decide whether lines lost are worth recovering first A place holder command which performs no function when executed by a program Set start time for an upcoming LOOP END command and number of iterations Set loop ending time r lt Enter gt or lt Down Arrow to finish lt Dn Arrow gt to access number of iterations lt Digit keys gt to set Sic B A Loop Structures Loops provide a powerful means of grouping repeated operations The ValveBank is limited to only 64 commands per channel so heavily used VALVE ON OFF and TTL ON OFF
15. R ON 00 00 16 00 TRIG LENGTH 00 00 02 00 TRIG LENGTH 00 00 02 00 On VALVE OFF 00 00 05 00 disables pending TRIGGER ON or OFF Off Sample TTL INPUT High Low t 0 5 sec 10 sec 15 sec 29 Run Mode Select P rogram lt Enter gt to Select Program Program 01 lt Cancel gt return to Main Menu Enter 2 digit program number 1 36 Press A Key To lt Enter gt to begin execution Start Program lt Cancel gt to abort Networking multiple ValveBanks If you are using multiple ValveBanks all Slave units will display Waiting For lt Cancel gt to abort Master Start see Configuration chapter p 43 A pulse from the Master ValveBank will now start programs running on all properly wired Slave units waiting on this screen Running Programs While a program is executing the current time and valve TTL output status is displayed Fractions of seconds are not displayed 01 T 00 13 08 V lt Time line See Manual Mode 10H 2CL XOL 4CL lt Status bar chapter for status codes While a program is running all manual key commands are functional except lt Enter gt to access the Manual Mode Special Features Menu See the Manual Mode chapter p 18 for information on commands available and a description of the status bar An additional code appears on the status bar while a program is running x Channel is done reached its END OF LIST command Program is completed when all four channels are done Oo
16. VALVE OFF 0 5M NaCl pH 7 0 01 T 01 00 00 00 res Salt 2 for 3 hours CH2 VALVE ON Glutamate Drue 1 for 1 minute 01 T 00 05 00 00 Channel 2 OMA AQ Channel 2 CH2 VALVE ON 02 T 04 00 00 00 CH2 VALVE OFF 02 T 00 06 00 00 BER CH2 VALVE OFF 0 5M NaCl pH 7 5 01 T 04 00 00 00 D Salt 3 for 1 hour CH3 VALVE ON Ca free saline Buffer 2 for 3 minutes 01 T 00 06 00 00 Channel 3 EET Channel 4 CH4 VALVE ON 02 T 05 00 00 00 CH3 VALVE OFF 02 T 00 09 00 00 CH4 VALVE OFF 1 0M NaCl pH 7 2 01 T 05 00 20 00 Salt 4 for 2 hours CH4 VALVE ON NMDA Drug 2 for 5 seconds 01 T 00 09 00 00 Channel 4 Bere Channel 3 CH3 VALVE ON 02 T 07 00 00 00 CH4 VALVE OFF 02 T 00 09 05 00 CH3 VALVE OFF Turn recording device on 03 T 00 09 05 00 CH3 TTL ON Saline Buffer 1 continuously 03 T 00 09 05 00 Channel 1 CH1 VALVE ON Buzzer upon completion 04 T 00 09 05 00 Type lt Enter gt to turn buzzer off CH1 BUZZER ON without disturbing program 05 T 01 00 00 00 END OF LIST at 1 hour CH1 END OF LIST Final buffer will run unattended until program reaches this point and ends 48 Gel Blot Washing User must calibrate time required to fill washing tray to desired wash volume and to vacuum drain tray In this example we use 30 seconds to fill and 20 seconds to drain Fill tray with Blocking solution 01 T 00 00 00 00 Channel 1 CH1 VALVE ON 02 T 00 00 30 00 Wait for 12 minutes washing CH1 VALVE OFF Drain 01 T 00 12 00 00
17. ValveBank 4 amp 8 II Y lveBark 811 AutoMate Scientific Inc 336 Baden Street User s Man ual San Francisco CA 94131 U S A 800 998 MATE Version II 1 4 6283 ey internationale 415 239 6080 faxe 415 239 6801 i a e maile info autom8 com wwwe http www automd com AutoMate Scientific Inc ValveBank4 amp 8 Part s 01 01 amp 01 08 For indoor use only from 5 to 40 C Do not get wet or subject to visible condensation Clean with a mild soap solution with a damp cloth only This equipment must be earth grounded Use any of the screws on the bottom or side of the case See page 15 IN Do not unplug valves while on Do not attempt to repeatedly cycle valves on and off in under 10 milliseconds If this equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Supply voltage ValveBank controller 12 V AC up to 2 4 Amps 50 60 Hz External power supplies USA amp Japan 110 V AC up to 0 26 Amps 60 Hz European 220 V AC up to 0 17 Amps 50 Hz Internal battery type 3V Lithium coin battery type BR2325 coin or CR2032 23mm x 165mAh Please replace fuse with standard 2 5 Amp M medium time blow fuse European CE Technical Representative C AutoMate Scientific Elisenstr 16 Leverkusen 51373 Germany ValveBank 4 amp 8 II ValveBank 811 User s Manual Version II 1 4 For research use only Jo
18. acing the ValveBank with external devices which receive 0 5 volt signals These TTL channels can be pro grammed to turn devices such as peristaltic pumps or recorders on and off Four additional TTL inputs can monitor such instruments as UV VIS detectors flow meters and level sensors These in puts can be programmed to trigger valve or TTL output activity A user created computer program can also use these inputs to control valves through the ValveBank See the Computer Interfacing chapter NOTE The ValveBank8 has only four TTL in and outputs TTL stands for Transistor Transistor Logic a 5 0 volt standard for representing on and off used by the ValveBank to control and monitor external devices See the Configuration Technical Notes chapter for more information and uses ValveBank Laboratory Applications Perfusion One or more ValveBanks can be programmed to precisely switch so lutions perfusing over biological samples for physiological research The ValveBank s circuitry is designed to open valves with low noise for electro physiology amplification Several advanced features have been built into the ValveBank s Manual Mode specifically for perfusion use Washing Combined with an AutoMate ported washing tray one or two ValveBanks can automate washing of electrophoresis gels and blots and developing of x ray or photographic film or prints You have complete control over which washing steps of your Western North ern So
19. alve fittings is intended as a gap filler for small openings between the threads and hole not as an adhesive Few glues will adhere to Teflon 41 EPROM Installation Instructions 1 Open the ValveBank case as described in Troubleshooting on page 46 Remove the last EPROM slowly witha flatscrewdriver orICremovernoting 1996 AutoMate Scientific the dimple orientation pin 1 on its left varveBank 1113 __ side Insert the new chip with the same orientation being careful not to bend any pins 2 Make sure that the ValveBank turns on then turn it back off before reinstalling the case top Save the old EPROM until you are completely satisfied with the new one EPROM s can be erased and recycled and they cost several dollars each So if you re happy with an upgrade it s worth the few pennies stamp cost for U S customers to drop them in an envelope back to AutoMate International or busy U S customers will be excused if they cannot return old eproms 42 Configuration Technical Notes The Configuration option on the Main Menu is used to set several user specific hardware and software options including multiple ValveBank networking normally open or normally closed valves normal or inverted TTL input and output logic and repeat running of user programs These settings can always be changed and will be stored indefinitely by the ValveBank even when disconnected from power They are factory pre set fo
20. anship and or material for ONE YEAR from the date of sale Any product that fails to perform as specified may be returned freight pre paid to the factory with a written explanation of the problem for examination and repair or replacement If it is defective AutoMate Scientific will repair or replace at our option the product without charge and return it to you If the examination indicates that non compatible fluid destructive environment accidental damage modification or abusive practices have occurred all labor materials and freight costs shall be at the expense of the customer Due to thenature of clinical laboratory applications AutoMate Scientific Inc will NOT accept the return of any products which have been used with HAZARDOUS MATERIALS or harmful environment This warranty is in lieu of all other warranties whether oral or written express or implied In no event shall AutoMate Scientific Inc or its licensor licensees be liable for contingent special direct indirect or consequential damages for the breach of any express or implied warranty or resulting from the use failure or malfunction of any product including damage to property and to the fullest extent permitted by law damages for personal injury even if AutoMate Scientific Inc has been advised of the possibility of such damages or if this warranty is found to fail its essential purpose AutoMate Scientific Inc s liability is limited to the reimbursement of
21. ating a need for service STOP If you ever have to remove the main system unit cover observe the following precautions A The power supply cord must be unplugged before the main system unit cover is removed Separe le cordon d alimantation et puis enleve le couvercle B Once removed the cover must be replaced and screwed in position before the power supply is plugged back in Apres le couvercle en place et remettre le cordon d alimentation Battery Warning Statement CAUTION There is a danger of explosion if the battery is replaced incorrectly Replace only with the same or equipment type recommended by the manufacturer Discard used batteries according to the manufacturer s instructions See inside of front cover for battery specifications ATTENTION Il y a danger d explosion s il y a remplacement incorrect de la batterie Remplacer uniquement avec une batterie de meme type ou d un type recommande par le constructeur Mettre au rebutled batteries usagees conformement aux instructions du fablicant See inside of front cover for battery specifications VORSICHT Explosionsgefahr bei unsachgemass em Austausch der Batterie Ersatz nur durch denselben oder einen vom Hersteller empfohlenen ahnlichen Typ Entaorgung gebraushter Batterien nach Angaben des Herstellers See inside of front cover for battery specifications 52 Warranty AutoMate Scientific Inc warrants its products against defects of workm
22. ch channel s tubing has been primed from its valve to this point Therefore the manifold should be positioned as close to the ultimate destination as possible while the valves can be some distance away The micro manifolds available from AutoMate Scientific are designed to be inserted without tubing directly into orifices in some perfusion chambers This absolutely minimizes dead volume resulting in switching times of under a second AutoMate Perfusion Systems include a blue disposable flow regulator which will increase dead volume if used improperly The regulator can either be eliminated if controlling flow rate is not necessary or positioned before the manifold to eliminate dead volume However this second method only controls the flow rate of individual solutions instead of all solutions when located after the manifold Therefore the researcher can either limit the flow of a single liquid or purchase flow regulators for each line A final method for reducing dead volume is to use smaller bore tubing although this may also reduce flow rate resulting in the same switching time as for larger tubing Small bore Teflon Tubing AutoMate Scientific s micro manifolds use 1 16 o d tube ports which can either be used with this size tubing or enlarged up to 1 16 i d for valve hose barb fittings Please inquire about AutoMate s Teflon tubing and nut and ferrule valve fittings for small bore use Also see the micro manifold instruc
23. combinations should be programmed as loops to avoid filling up all of the available program lines Loops on the ValveBank can also be nested used inside one another up to four deep Outer Loop Start Inner Loop 1 Start Inner Loop 2 Start u Nested Inner Loop 2 End Loops Inner Loop 1 End Outer Loop End Each channel is limited to 16 loops Multi channel Loops You may want to repeat command sequences from several channels To do this you must include a LOOP START and END combination at exactly the same times in all channels to be included Channel 1 Channel 2 Loor START 00 00 00 00 Loor START 00 00 00 00 VALVE ON 00 00 00 00 VALVE OFF 00 00 05 00 VALVE ON 00 00 05 00 VALVE OFF 00 00 10 00 Loor EnD 00 00 12 00 t Loor END 00 00 12 00 TTL Input Commands TTL RESPOND Valve will mirror its channel s TTL input after the specified time while TTL input is High valve will be Open TTL Low Closed See Configuration to invert High Low logic TTL IGNORE Valve ignores TTL inputs after specified time Turns off a TTL RESPOND command 27 TRIGGER ON TRIG LENGTH TRIGGER OFF Must be followed by a TRIG LENGTH command which is automatically inserted and deleted by the ValveBank The TRIGGER ON command instructs the ValveBank to begin watching the TTL input associated with its channel When that TTL input goes High that channels valve will open like a TTL RESPOND command but only for the amoun
24. ctrolytic corrosion 3 Donot operate AutoMate valves with homemade controllers delivering over 12V DC or attempt to cycle them rapidly lt 10ms 4 Do not exceed the rated operating pressure gt 100psi Before returning any valves to us please check 1 That it was purchased in the last 12 months AutoMate and valve manufacturers record each by serial number 2 That the valve is faulty and not the controller channel find a valve that works in a particular output channel and try the bad valve in that port to see that it still does not function 3 If it is leaking see 1 above and the section below If it qualifies for all three of the above AutoMate will accept the valve for subsequent return to the factory Repair or replacement is at their discretion as AutoMate does not have the ability to open the valves to determine the cause of trouble We may be able to loan replacement valves which may be billed if the valve factory finds abuse on the user s part and refuses to honor the warranty Stuck pinch Valves Occasionally pinch valves will stick closed Usually this is a sign that they are about to completely fail but sometimes they can be rejuvinated by simply prying them open First energize turn on the valve then using a flat screwdriver gently force the white plunger open where it normally pinches the tube closed The valve may begin functioning normally Also pinch valves will not usually work without a piece of p
25. dicate when Select Program you are creating a new program in i an unused slot Since you intended to BLANK PROGRAM start a new program ignore the beep and continue lt Enter gt to Select Channel Select Channel lt Cancel gt return to previous screen Channel 1 or Save Program after editing Enter 1 digit channel number 1 4 8 21 Editing a Program Line number see lt Up Dn Arrows gt below 01 T 00 00 00 00 lt Time line HH MM SS 00 Ch1 END OF LIST lt Current channel amp command line Time line Set time value using arrows and digits Represents time event will occur NOT duration Move the cursor up down one line at a time When cursor is on the line number Browse up down two lines one event at a time Note You cannot scroll past the END OF LIST dq Time line Move between time digits amp line number gt Cmd line Change commands see next chapter After programming each channel press lt Enter gt ENGEL or lt Cancel gt to return to the Select Channel eer screen and repeat the programming process for all four channels in a program CANCEL lt Cancel gt from Select Channel returns to the Save Program screen if you have made any changes Saving a Program Save often entering long programs None of the changes you make are permanent until you save them When you finish editing a program the ValveBank will ask you if you want to save the changes lt Enter gt to make selection lt Cancel
26. en alcohol three times each through the tip If the Multibarrel Perfusion Pencil is filled with pure salt solution leaving it in the syringe overnight will not usually cause the tip to block However if it is used for high viscosity fluids flushing after each use is recommended Cutting The Removable Tips are shipped with 1 5 3 8 cm length polyimide needles They are intended to be cut to a desired length by rolling a razor blade on them against a hard surface However this may leave a small crack or barb on the tip To get a flat cut score the coating of the needle with a ceramic cleaving stone or a diamond cutter and pull directly apart making sure not to pull at an angle You may notice a larger outer tube enclosing the lower part of our smaller 100um needles This is simply for added rigidity and can be cut if needed 14 Back Panel 12 V AC Power up to 2 4 Amps 50 60 Hz Keypad calc we ante wedt 12vpc Valves ValveBank 8 Part 01 08 Serial RS 232 Ports For Research Lse Only o Made in California USA 11 Inter ace Port Digital TTL port See page 45 12 V DC valves Serial RS 232 Ports In computer and Out next device n chain See page 32 Case amp Valve Grounding This equipment must be earth grounded Use any screws on the bottom or side of the ValveBank case for grounding Order AutoMate part no 01 05 Low noise valve and case grounding package for an ext
27. future use Likewise programs written or modified in the ValveBank can be sent back to a host computer for storage editing or printing Select Transfer lt Lt Rt Arrows gt move cursor lt Enter gt select menu option gt SEND RECEIVE lt Cancel gt abort to Main Menu Follow the instruction found in the EasyCode manual for preparing your computer to send or receive information with the ValveBank Press lt Enter gt on the ValveBank when EasyCode tells you it is ready ValveBank gt Computer send Select Transfer gt SEND RECEIVE Select SOURCE Program 01 EMEP nk receive Start a receiver first Load a ValveBank user program then transmit it to a host computer ENTE A A O 3 D Cc v N lt g3 Q Select Transfer Receive a program then ENTER SENIL gt RECEIVE save itina ValveBank Destination program slot Program 01 ENTER lt Cancel gt will abort during transfer CANCEL See page 45 for a serial cable diagram Uo 2 File Mode ENTER Make selection gt COPY Program ERASE Program Return to Main Menu COPY Program will request a SOURCE program with a warning if the source is blank and the DESTINATION program with confirmation for Overwrite if that program slot is already being used ERASE Program requests the program number to erase then asks for verification before completely erasing it Clear All Memory This command should be used with extreme ca
28. hannel Change times and commands as above Move to next line enter time and command BEEP Type lt Cancel gt 2x to jump to Save program screen UNSER TEINE Type lt Enter gt to finish DELETE LINE saving program lt Cancel gt always aborts Commands Time values for each event in a channel must be greater than or equal to the previous event s time 01 VALVE ON 00 00 10 00 02 BEEP 00 00 10 00 Legal structure 03 VALVE OFF 00 00 20 00 01 VALVE ON 00 00 20 00 02 VALVE OFF 00 00 01 00 Illegal structure Error checking is built into the Save Program screen to check for these illegal structures Times represent when event will occur NOT durations Turn valve on at the specified time Turn valve off at the specified time Turn TTL output on at the specified time Turn TTL output off at the specified time Turn buzzer on at the specified time Turn buzzer off at the specified time Example sound buzzer for several minutes if you must be alerted for a particular step but may be away from your experiment for a few minutes After entering an On Off command its counterpart is automatically guessed on the next command line Sound buzzer for 1 second needs no Off command Moves current line down and inserts a new line in its place for adding new commands in existing programs Leaves a BLANK placeholder Deletes the current program line and moves next line up This is permanent 25 END OF LIST If E
29. inch tubing installed Valve Cables One problem that is easy to diagnose and fix is a broken cable connection Use any ohmmeter to measure the resistance across the inner pin and outer barrel of the RCA plug end of the valve cable while unplugged from the controller A resistance of zero ohms indicates a short or infinite resistance means one of the wires is broken in the cable assembly This occurs most often at the joint between the heavy black cable and the valve lead wires under 29 the heat shrink tubing inside the outer black sheath a few inches from the valve itself This can often be reached without completely disassembling the valve enclosure Simply 1 remove the screw inside the black valve box holding the plastic sheath in its hole 2 Valve enclosure shown without front plate the black tape at the top of EEE x e sheath and peel it back a couple of inches find the offending cable 1 pe 2 trace it back from the bad valve Plastic split loom cable sheath and 3 carefully slice its heat shrink tubing longitudinally to remove 3 it The wires may have come apart Heat or broken here inside the next seal piece of electrical tape isolating the cable two leads otherwise within the cable or valve itself which can be further determined with the volt ohmmeter If you find the break resolder the connection wrap the inner wires in separate pieces of electrical tape retest the valve and reassemble the
30. irs or running a program 3 way Valves The ValveBank supports 3 way single solenoid valves 2 wire Program the ValveBank as you normally would for 2 way valves after determining which of two inlets or outlets are opened with VALVE ON and VALVE OFF commands These can be reversed with the Normally Open Closed Configuration settings described above da TTL Input Output Logic These two settings allow the user to invert the TTL logic on incoming and outgoing TTL port signals for use with TTL ON and OFF commands and Manual Mode TTL is an electronics abbreviation for Transistor Transistor Logic representing simple signals being transmitted as either 0 volts ground or 5 volts Usually a 5 volt signal called High represents ON or active and 0 volts Low represents OFF This Configuration setting inverts the standard ON OFF convention to Low ON and High OFF to suit the particular electronic needs of a custom lab arrangement TTL Output S lt Enter gt to next screen IN ZN 3N 4N lt Cancel gt return to Main Menu lt 1 4 gt or 8 toggle each channel TTL Port Standard female DB 25 connector diagram view back of ValveBank Pins 11 13 Pins 3 10 Pin2 45V TTL outputs 1 3 TTL inputs 1 8 pn 13 25 mn 14 18 Pins 19 25 reserved DO NOT CONNECT TTL outputs 4 8 Serial Cable ValveBanks communicate with EasyCode and other serial sources using a null modem cable or standard serial
31. l Perfusion Pencil sold separately Replacement Tips 04 04 xxx 4 into 1 Pencil with tip Part No Description 04 08 xxx 8 into 1 Pencil with tip 04 16 xxx 16 into 1 Pencil with tip xxx 100 250 or 360 um 04 xxx specify 100 250 or 360 um Epoxy or silicon glue may be needed to completely seal connections between valve tubing and the manifold tip in ports Tighten removable tip for minimum dead volume a One of the eight lines may be connected to vacuum through a valve programmed to suck the dead volume clear between solutions Chemical Information The tubing inside the manifold body is polyimide nylon The largest connection tubing is vinyl The Removable tips include a medical grade polypropylene Luer lock fitting with a fused silica quartz needle coated with polyimide These materials are resistant to most acids bases and organic solvents To avoid dust contamination we recommend pre rinsing the Perfusion Pencil and Tip with distilled water Also it is good practice to discard the first few microliters of solution before using the device The Perfusion Pencil and Removable Tips are shipped non sterile They can be chemically sterilized or autoclaved However repeated autoclaving may weaken the adhesive bond between the Lure lock fitting and the needle Maintenance You can expect several years of useful lifetime for your tip if you wash it daily Use a syringe or vacuum to pull first water th
32. lveBan G nenne 43 ING RUN ee nase nee 43 Normally Open Closed Valves anssen eeen Ad Fa ne at ae eo onan 44 TIL ipul Oaa LO ee 45 TETOR sea un 45 e Gi e a r r eee Te eT eee eee nT ee eee ary 45 Troubles hoo aa ee een 46 EasyCode Macintosh amp PC Software vennen vens oons eens een 47 Sample Programs sassinoro e 48 Safety Instruc HONS nennen een 51 Wirany au 53 Introduction PLEASE SET ASIDE SOME TIME TO READ THIS MANUAL BEFORE USING YOUR NEW VALVEBANK SYSTEM The ValveBank is a very unique and sophisticated programmable instrument It will be difficult for you to get the most out of its fea tures without reading this manual Thanks FOR RESEARCH USE ONLY The ValveBank is designed for solution switching use in research applications ONLY AutoMate Scientific Inc cannot be responsible for injury or death resulting from medical or pharmacological use Hardware Overview The ValveBank 4 and 8 are stand alone programmable valve and pump controllers with 10 millisecond accuracy Programming and manual control is accomplished through a 16 key membrane key pad back lit liquid crystal display and easy menu driven user in terface The user can store up to 36 programs 18 for a ValveBank8 totaling 64K of battery backed up stored even when unplugged RAM memory The ValveBank is designed to drive 12 volt DC solenoid valves plugged into RCA jacks on the back of the box Four TTL outputs are provided for interf
33. ly after VALVE ON s and monitor the four TTL outputs on the ValveBank VALVE ON 00 00 12 55 TTL ON 00 00 12 55 TTL OFF 00 00 13 55 for a one second pulse Use other pulses for valve closures or important timing milestones Both the Valve Driving and Feedback strategies discussed so far can be combined with normal ValveBank programming in separate channels Slave Mode Computer Interfacing See the next chapter about wiring multiple ValveBank s together in a Master Slave configuration Your computer can simulate a Master ValveBank and start programs running on Slave ValveBanks synchronized with other activities on your computer Connect your computer TTL output pin to input pin number one on all ValveBanks To activate the ValveBanks send a three pulse on off square wave Signal into Slave ValveBank s will TTL Input pin 1 start their programs here 5 10 ms minimum OV t Note TTL Input 1 can still be used on slave ValveBanks after their start signal 39 Techniques Dead Volume Dead volume should be minimized in order to switch solutions as rapidly as possible Dead volume for this purpose refers to the volume in the single piece of tubing between the manifold and final destination perfusion chamber 96 well plate etc A certain amount of time must pass for a new liquid to clear the previous one from this final piece of tubing Solution switching takes place at the manifold assuming ea
34. ngerous voltage points or short out parts that could result in fire or electric shock Never spill liquids of any kind in the product 5 This product should only be connected to the AC power source indicated on your product system s information label If you are not sure of the type of AC power available consult your dealer or local power company Only connect this product to a power outlet that matches the power requirements of this product 6 Do not allow anything to rest on the power cord Do not locate this product where people will walk on the cord 7 If you have to use an extension cord with this product make sure that the total amperage rating of all equipment plugged into it does not exceed the amperage rating of the extension cord Also make sure that the total of all products plugged into the main AC power outlet does not exceed 15 Amps 8 Unplug your product from the main electrical power before cleaning Do not use liquid cleaner or aerosol cleaners Use a damp cloth for cleaning 9 Do not use this product near water 51 10 Unplug this product from the main power outlet and call for service under any of the following conditions A If the power cord or plug is damaged or frayed B If liquid has been spilled into the product C If the product has been exposed to rain or water D If the product has been dropped or the cabinet has been damaged E If the product exhibits a distinct change in performance indic
35. osure as follows a These valves have not been exposed to chemicals other than H O except as described above b All applicable Safety and Handling data sheets for the above described chemicals are enclosed c We accept full responsibility for any injury to AutoMate employees or employees of the valves original manufacturer caused by handling of the residues of chemicals contained in or on the valves to be returned 7 An authorized signature title and date AutoMate will gladly fax you a form with easy blanks to enter the above information We will process your return and notify you of further information as quickly as possible Leaks amp Replacing valve fittings The second bane of liquid delivery apparatus These hints may help 1 Make sure the valve is turned off as opposed to leaking 2 See all of the guidelines in the previous Faulty Valves section especially 1 3 Identify whether the valve leaks constantly under pressure inside or outside Try backflushing if an internal leak 4 Check and replace obvious loose or worn tubing 5 Outside leaks usually occur at the fittings and can be stopped with a bit of silicone glue epoxy super glue Cyanoacrylate or Teflon tape around the threads of the dry fitting screwed back into the valve Be sure not to use too much glue or tape as they can easily cause clogs or leaks of their own Overtightening can also cause the plastic fittings to leak hand tighten Glue on v
36. r a standard laboratory config uration and AutoMate normally closed valves The user may never need to make changes to these options Networking multiple ValveBanks The SLAVE option is used when two or more ValveBanks are networked together The MASTER box sends a start pulse see pg 30 to synchronize all SLAVE boxes This allows programs to be written across several ValveBanks which each control their own valves and TTL outputs When started in sync these ValveBanks operate as though running a single program written for many valves Multiple ValveBanks should be connected through their TTL In Out Ports by a cable supplied by AutoMate See page 11 for a diagram of a double ValveBank arrangement Select Box Type lt Enter gt to make selection amp continue gt MASTER SLAVE lt Cancel gt return to Main Menu lt Lt Rt Arrows gt toggle modes Repeat Run Repeat Run allows a user to run a program continuously When activated Repeat Run instructs any running program to start over immediately when finished User programs are normally limited to 99 hours so the only way to set up a program to run for more than four days is to write a sequence up to 99 hours long which will be repeated indefinitely each time it finishes Turn Repeat Run lt Enter gt to make selection amp continue ON gt OFF lt Cancel gt return to Main Menu lt Lt Rt Arrows gt toggle modes 43 Normally Open Closed Valves This setting configures the Val
37. ra grounding wire attached to each individual valve extending back to the controller Each of this item grounds four 4 valves Please order two for eight channel perfusion systems This item must be ordered at the same time as the valves Connect the ground wire from the valves to a screw on the bottom or side of the controller case 15 Main Menu Select Function Run Mode This notation is used throughout the manual to represent the ValveBank s liquid crystal display The ValveBank8 uses a 4 line by 16 character screen so examples will differ slightly The ValveBank displays two introduction screens every time it is turned on These can be advanced by pressing any key lt Up Dn Arrow gt keys Scroll through 6 menu choices Run Mode Run an existing program Manual Mode Open close valves manually Edit Program Write a new program or edit an existing one File Mode Copy and erase programs Serial Mode Transfer programs to from your personal computer Configuration Adjust preferences Each Mode is discussed in its own chapter later in the manual lt Enter gt key ENTER Selects current menu choice 16 ValveBank AutoMate T Manual Mode V 1C 2C 3C 4C 5C 6C 7C 8C Manual Valve Commands CANCEL Pause End Program ALL Valves Close ENTER Resume Buzzer Off Special Features Toggle 1 8 Control Valves or TTL outputs Open Close Valve or TTL outputs ALL Open
38. re Entering 99 on the ERASE Program screen will ask for verification to ERASE ALL MEMORY Although AutoMate Scientific has thoroughly tested the ValveBank s internal software like most computers it is possible for the ValveBank to Crash and freeze up In extremely rare circumstances a bad crash can write garbage data in random bytes across the memory When you turn the ValveBank back on you would find strange commands and times stored in some programs used or unused Again this is very rare However if this occurs you may wish to clear all memory and re send programs stored on your Macintosh or PC in the EasyCode program to the ValveBank by Serial Mode assuming you have EasyCode Clear All also resets all Configuration and Manual Mode Special Functions settings to their factory defaults 33 Computer Interfacing Using the AutoMate EasyCode software package a user can create ValveBank program sequences on a PC and send them to the ValveBank s memory Programs are then run on the ValveBank However some users have already invested long hours in developing custom timing and data collection programs on their personal computers to automate their research The ValveBank can still be invaluable for these users both for Manual Mode calibrating and as an advanced valve driver under computer control Valve Driving With four TTL pins from an interfacing board on a personal computer the user can turn valves on and off through
39. roblems please observe these guidelines 1 Clean your valves after each use Accumulated debris or precipitate will rapidly cause leaking valves and seals Use an in line filter if you expect a chance of particles 5 um max in your solutions Put your AutoMate controller s programmability to good use by writing a washing protocol At the end of the day fill each reservoir with cleaning solution distilled water or appropriate liquid and press Start A protocol can be easily written to flush each valve in sequence Saline solutions allowed to dry in stainless steel valves will cause them to rust This is considered improper use We also recommend rinsing your valves once before their first use Beware of flaking Teflon tape or silicone fitting glue Large debris are often the cause of valve failure Try backflushing a valve that is suspected to have failed for this reason by connecting a lightly pressurized liquid to the outflow of the valve while it is energized Sometimes this ejects the clog No Draino 2 Use appropriate valves for a given application Use of caustic or corrosive liquids with our stainless steel valves immediately voids their warranty and the factory will notice as soon as they open them Please request our Teflon isolation valves if you plan on using such solutions We even recommend isolation valves for highly conductive saline solutions and or elevated 38 temperatures because of the increased probability of ele
40. rvoirs 5 Insert short pieces of 3 16 i d tubing over tops of drippers then insert over reservoir nipples 6 Attach dripper tubing to upper valve barb cut as desired but see note above first 7 Insert green clamps on short pieces of 1 16 tubing 8 Connect tubing between bottom valve barbs and manifold 9 Connect flow regulator tubing to manifold outflow and your perfusion chamber downsizing if necessary 10 Plug numbered valve cables into ValveBank ports Calibrate flow rates by running one line into a graduated cylinder for a fixed period of time Chromatography st WI Fraction collector or waste Gel Blot Washing System vacuum trap C 10 Without a pump flow rate is determined by relative height of reservoirs 1 Position reservoirs above column ringstand 2 Attach valve unit to col umn stand 3 Run tubing from upper valve barbs into reser voirs 4 Connect short pieces of tubingbetweenlowervalve barbs and manifold 5 Connect manifold outflow to your pump with 1 16 i d tubing 6 Pump outflow connects to column inlet as usual 7 Plugnumbered valve cables Flow rate is determined by relative height of reservoirs 1 Position reservoirs above washing area 2 Attach valve unit to ring stand 3 Run tubing from upper valve barbs to reservoir nipples 4 Connect tube from lower valve barbs to tray inlet
41. s 5 Connect tray outflow to fourth upper valve barb 6 Connect this valve s outflow to vacuum trap 7 Plug numbered valve cables Multiple ValveBank Western Washing System 5 input solutions 3 drains Waste Primary amp Secondary s recovery i vacuum traps Plug 1 Double ValveBank cable Secondary ValveBank s Master ValveBank Follow the instructions on the previous pages for setting up the ringstand reservoirs valves and tubing for your specific application Connect each bank of valves to a ValveBank Designate one ValveBank as the Master and all others as Slave units Connect the Valvebank s together with the provided cable with plug 1 to the master s TTL In Out Port and plug 2 in the secondary unit Make a note of which ValveBank is master it will be used to start all slave units See the Configuration Technical chapter for more details 11 Teflon Micro manifold Use Your valves should be equipped or retrofitted with 1 16 o d outflow hose barbs Micro manifolds are shipped with a short piece of 1 16 o d tubing in each hole inputs and outflow You can either connect the valves to manifold inports with a piece of 1 16 i d tubing over both the valve barbs and short pieces of manifold tubing Valve with 1 16 o d barb l 1 16 i d tubing over barb and over manifold short tubing l Manifold with short pieces of 1 16 o d tubing in each por
42. s reservoir is empty see above paragraph AutoPrime System The AutoPrime Perfusion System is designed to deliver oxygenated or other gas saturated solutions without administering stale liquid which has remained stationary in the tubing long enough to lose its oxygenation Unless one uses hard walled i e Teflon tubing gas will escape through the tubing leaving the stale solution with a ruined pH and gas concentration The 8 channel AutoPrime System uses eight valves per four liquids 37 with the second set used as flush valves to waste Shortly before a solution is to be switched on its flush valve opens and re primes the upper tubing with fresh solution from the reservoir down to its delivery valve where a tee fitting is located connected to the flush valve The flush valve is then closed and the delivery valve can be opened The previous solution in a delivery sequence can continue to flow while re priming occurs Faulty Valves The bane of all liquid delivery apparatus Despite AutoMate s best efforts and pursuit of high quality valve manufacturers some valves fail How often have space shuttle launches been delayed due to valve problems We can only pass along our valve supplier s one year warranty with their disclaimer Improper use or mishandling of the units in the opinion of the manufacturer voids this warranty their exclamation mark too In order to minimize valve p
43. se three choices are left for you to decide how to best mount the calc for your use on a tabletop with feet or stuck to any surface with removable Velcro or magnetic tape The ValveBank main unit also has two options Four additional feet are included for simply leaving the ValveBank on the bench top or rack mounting brackets and screws can also be purchased separately ValveBank Accessories PowerStrip The AutoMate PowerStrip resembles a conventional 4 socket electrical powerstrip but actually contains an additional circuit board for interfacing with a ValveBank Each of the four outlets is individually user controlled through the ValveBank A cable from the PowerStrip plugs into the ValveBank TTL port and another plugs into a standard 110V wall socket Any piece of lab equipment pumps recorders rockers etc can be plugged into the PowerStrip and easily controlled by user programs or manual mode EasyCode Software for Macintosh and IBM PC Pressure Regulated Perfusion System AutoMate Scientific s EasyCode software for the Macintosh and IBM PC compatibles allows you to program On Off sequences through a simple graphic timeline interface and then send and receive these programs to the ValveBank through its serial port See page 47 AutoMate Scientific now offers a Pressurized Perfusion Upgrade with either four eight or sixteen channels for e Steady flow rate e Microinjecting e Faster Switching e
44. sef Kewekordes Il Heath Lukatch David Ilstrup Gabriel Stern Brian Greenstone EPP 1996 2000 AutoMate Scientific Inc All rights reserved WARNINGS Do Not Unplug Valves while Energized The resulting voltage spike is much like a car spark plug and will destroy circuit board components Do Not Clean the ValveBank Screen With Paper Towels It Will Scratch Use a cloth with a 50 alcohol solution This equipment must be earth grounded Use any of the screws on the bottom or side of the case Table of Contents IJntr oduchion nenn 3 Hardware Og a 21 RATE NEBEN IRRE E IE ER DER EINREISE ARE 3 YalveBank Laboratory Apphoatonsse ua 4 Ser A E TO Or aaruantaenareavesren 4 Gate OF your Valvyebank SCEE se 4 Mounn Opions SASSEN eeen bene 5 Valve DAAK AC COS re 5 EasyCode Software for Macintosh and IBM PC ee 6 bier lock Valves amp Syringe Reservoir na 6 Pressure Regulated Pertision Sy steieren kun 7 M ltebarrel Perfusion Benel I anne 7 System Setup Diagrams ssssesiasacssccsavesssaeisdiavnsssiaoresesssieasssotasseesosacnsbsaiers 8 Cron Eh Tee ee eene 8 Standard Teflon Perfusion System without pressure cee 9 Sancenet nee an ee ee een 10 Gel Blot Washing vet een 10 Telon Micro maniiold Denen 12 Pertusion Presse Rn iri e E EAA 13 M lt barrel Perfusion BeOne i 14 Case GE A SON nee 15 Dek 11 gt S E E E AEE 15 Main Ment ssssicieassesscacss canisnsanesseitions Seales ceadssuasdtantens edesshastdesansscsaninsesuebie
45. sheath following the above instructions in reverse order Valve Returns amp Replacing a Valve If you need to return a defective valve you may either return the entire valve assembly for repair evaluation or remove and return the individual valve from its case This only involves five screws remove the two outer plate screws holding all of the valves into the black metal case the single screw holding the plastic sheath into its underside hole the electrical tape at the top and bottom of the sheath the offending valve s cable from the plug end up through the valve casing and finally the two screws holding the valve on to the front plate Please package the valve s carefully and ship freight prepaid to AutoMate s address on the back cover of this manual No RMA number is needed as long as you include a note with the following 1 Your name company institution name phone number and an address to return the repair to 2 Your original invoice number and date if available 3 A sentence stating the nature of the problem and any steps 40 you have already taken to fix or identify it Does the valve energize click De energize Open close Leak 4 Whether the valve was driven by any device other than an AutoMate controller and its operating and holding voltages 5 The application used with the valve along with the exact chemical s and the duty cycle pulse rate if used 6 AStatement of Chemical Exp
46. t or for even less dead volume use a short piece of 1 16 i d tubing over the valve barb and piece of smaller 1 16 o d tubing inserted inside the valve tubing and manifold ports you may need to supply extra small tubing for this option Valve with 1 16 o d barb V Short piece of 1 16 i d tubing over valve barb V Small diameter 1 16 o d tubing inserted inside valve tube and inserted inside manifold ports V Manifold without connector tubing 1 16 i d holes Cutting the tubing at an angle will make insertion easier Cutting the tubing square however will minimize dead volume when inserted as far as possible into the manifold being careful not block at the point of convergence Remember to keep all tubing as short as possible 12 Perfusion Pressure Kit sold separately 09 04 amp 09 08 Press regulator knob down to lock Pull up to unlock Brass input connector hose barb size 1 4 i d Max input pressure 100 psi Individual air lines can be adjusted or turned off 5 micron bowl filter MER Be very careful with these Press bottom button to empty kirn plastic hose connections B ii g E E fi fi E i i E i ii Syringes may also be placed in a water bath for temperature control 2 way stopcocks and lure lock to 1 16 hose fittings are included for each channel to connect syringes to hose Hoses should continue from syringes to valves Hie Multi barre
47. t of time specified by the following TRIG LENGTH command If the TTL input is already High when the TRIGGER ON command is processed the valve is opened immediately and timed according to the TRIG LENGTH as usual TRIGGER ON only triggers once Use loops to repeat trigger Either a VALVE ON or VALVE OFF command will discontinue a pending TRIGGER ON command and override any TRIG LENGTH time remaining Cannot be entered by itself Automatically inserted or removed by the ValveBank following a TRIGGER ON command Specifies amount of time to hold a valve open when TRIGGERed Cannot be deleted changed or separated from its TRIGGER ON partner with an INSERT LINE You must change or delete its TRIGGER ON to remove it To be useful TRIGGER OFF must be preceded by a VALVE ON command Once encountered the TRIGGER OFF command instructs the ValveBank to watch its TTL input like a TRIGGER ON But when the TTL input goes High the ValveBank closes that channel s valve assuming it was open TRIGGER OFF is discontinued by a VALVE ON or OFF just like TRIGGER ON See the Timing Diagram on the next page and the Computer Interfacing chapter for more TTL input information 28 Timing diagrams Sample TTL input is bottom graph TTL RESPOND 00 00 05 00 TTL IGNORE 00 00 16 00 On Off TRIGGER ON 00 00 05 00 TRIG LENGTH 00 00 05 00 VALVE ON 00 00 00 00 TRIGGER OFF 00 00 05 00 Valve output TRIGGER ON 00 00 04 00 TRIGGE
48. the ValveBank TTL inputs with their own custom programs Why not connect valves directly to your computer Several reasons 1 Most computer interfaces provide 5V signals not strong enough to drive most valves 6 to 12V DC and up 2 Computer interface signals are often too noisy for electro physiology amplification The ValveBank is designed with special low noise circuitry to minimize interference with high gain amplification 3 Finally many solenoid valves are designed to be opened with a short full voltage pulse then held in with a lower voltage to keep the valves from heating The ValveBank automatically provides dual voltage hold in even when being controlled by an external computer The easiest way to drive valves through the ValveBank is to write a short program with a TTL RESPOND command in each channel at time zero and another TTL RESPOND at time 99 hours to give the program length Then set Repeat Run ON in the Configuration menu Connect your four interface signals to the four TTL inputs on the ValveBank see the Technical Notes chapter and run this program The ValveBank will open and close valves exactly mirroring the computer signals with 10 millisecond accuracy 34 Feedback to a recorder It is also possible to program the ValveBank to provide synchronization signals back to a computer or recording device to reference exactly when valves were opened or closed Just include TTL ON commands immediate
49. tions on page 12 Cutting Teflon tubing at an angle makes it easier to insett 36 Backflow When a manifold is used in a plumbing arrangement none of its ports can be left unconnected or liquid will simply flow back wards out of the opening Small green hose clamps are included with AutoMate Perfusion System drippers which can be easily at tached to short pieces of tubing to act as plugs for unused holes Likewise one must be careful not to open any valve whose reservoir is completely empty or inflow tube is disconnected Ordinary solution pressure in other lines will force liquid back up the outflow tubing through the valve and either back into the reservoir or out the disconnected inflow onto the floor Again clamp off any unused lines at the manifold or be sure all valves are closed while changing connections Syringe Reservoirs AutoMate offers 30ml syringes photo on page 6 as alternatives to its 100ml polypropylene reservoir cups Connected directly into luer lock fittings in the valves this method eliminates wasted solution in tubing between the reservoirs and valves Small 2 way stopcocks are included when this option is ordered These are placed between the syringe and valve fitting in case syringes must be disconnected before completely empty Simply loosen the luer lock from the valve fitting and remove the syringe and stopcock together These stopcocks are also useful if there is a chance of a valve opening after it
50. ual Mode V ch2 OO ia A OO 1OH 2CL 30L 4CH are ga gt un un pie lt e pR T m Manual Mode V 1CL 2CL 3CL 4CL Manual Mode V 10L 20L 3CL 4CL Manual Mode V 10L 2CL 3CL 4CL gt Manual Mode I 10L 2CL 3CL 4CL Manual Mode T 10H 2CH 3CH 4CH Manual Mode T 10L 2CL 3CL 4CL Manual Mode T 4 10L 2CL 3CH ACH Manual Mode V 10L 2CL 3CH 4CH Manual Mode V 10L 20L 30H 40H DSE LEE No 0 Keys pressed 8 Closed Low Result underlined on next display Starting configuration Open valves 1 and 2 Close valve 2 Switch to TTL mode Open All All TTL output s High Close All All TTL output s Low again TTL 3 and 4 On Switch back to Valve mode Open All valves Exit to main menu All valves amp TTL close Edit Program Mode The Edit Mode is used to create and modify user programs Each program is separated into four channels Each channel controls its own valve and TTL outputs and responds to its TTL input The ValveBank4 holds 36 programs ValveBank8 holds 18 in battery memory with each channel allowed a maximum of 64 commands Control diagram of a user program Channel 1 To begin select a program slot to work in Select Program lt Enter gt to Select Program Program 01 lt Cancel gt return to Main Menu ee Enter 2 digit program number 1 36 Underline represents the blinking cursor where you are typing The ValveBank will in
51. uthern Coomassie Blue and Silver staining runs are auto mated and which are handled conventionally Unattended washing can save a busy lab countless hours of tedious monitoring Liquid Chromatography A ValveBank can be easily programmed to automate solution selection in multi step ion exchange gel filtration or affinity chro matography runs with excellent reproducibility The ValveBank is especially useful in preparative work when a protocol has been established and programmed to repetitively isolate your valuable molecule User s guide notation Throughout this manual ValveBank keys for the user to press are shown in bold type lt Enter gt or lt Cancel gt or as keys Care of your ValveBank screen Avoid scratching the screen cover by cleaning it with a 50 alcohol water solution using a cloth never paper towels Mounting Options amp Assembly We would like to explain the small items in the plastic bag included with your ValveBank The first difference in the new ValveBank8 you may notice if you were familiar with the previous generation is that the keypad and liquid crystal screen are now separated by cable from the main unit in what we call the calc Now your ValveBank can be mounted away from your experiment area even rack mounted but its control can be placed nearby We have included four rubber feet six inches of Velcro and six inches of adhesive magnetic tape for several calc mounting options The
52. veBank for use with user supplied Normally Open valves AutoMate sells only Normally Closed CL valves valves which are closed to fluid flow when NOT powered which is the default for this setting Inverting a channel to Normally Open OP represents that valve receiving power on a VALVE OFF command and no power when ON VALVES Normal Ly lt Enter gt continue to next screen 1 4 CL CL CL CL lt Cancel gt return to Main Menu lt 1 4 or 8 gt toggle each channel NOTE Normally open valves The ValveBank must supply power continuously to keep normally open N O valves closed even while no program is running If the Configuration setting specifies N O valves then the ValveBank will turn 12V on for all N O valves as soon as it is powered on and whenever a user program ends This is intentional to insure solutions do not flow unexpectedly Therefore N O valves will be held closed as long as the ValveBank is on They should not heat up appreciably and this should not be harmful to the ValveBank however if your reservoirs are empty or solutions are otherwise blocked ie manual thumbwheel valve you may wish to stop the ValveBank from supplying power to the valves while the ValveBank is turned on Choose Configuration from the Main Menu and temporarily switch all valves to normally closed The ValveBank will stop supplying power to the valves thinking they are normally closed Set them back to normally open before filling your reservo
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