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Evolution 500 LC Software Manual

1. 19 01 98 66 55 11 35 432 11 CLOCK TIME HOURS 16 MINS 32 DATE DAY 25 MONTH 03 YEAR 98 BETO CANCEL ACCEPT PAGE 19 01 98 11 35 EHT CALIBRATION EHT TABLE NORMAL EHT TABLE NORMAL HIGH ABS FLAT SETUP CALIB PAGE RATE 19 01 98 66 55 T 11 35 432 1nm WAVELENGTH CALIBRATION CALIBRATION USING Hg LAMP CALIBRATION COMPLETED SETUP CALIBR PAGE RATE 19 01 98 66 55 11 35 432 1nm INITIALISE INITIALISATION TYPE OPTICS INITIALISE WITH D2 OFF INITIALISE OPTICS BASELINE SETUP INITI PAGE ALISE 19 01 98 66 55 11 35 432 1nm LAMPS TUNGSTEN ON HOURS 54321 ENERGY 91 2 OFF HOURS 1234 ENERGY 096 Hg OFF HOURS 2 ENERGY 0 SWITCH SWITCH RESET D2 Hg HOURS Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual This section describes how to set up the instrument The main system setup options are available directly from the SETUP function key on the HOME page SETUP Page From the SETUP page move the cursor to the required option using the Up Down Arrow keys Select the option by pressing the ENTER key r 1 CLOCK Switches to the CLOCK page SETUP EHT Switches to the EHT CALIBRATI
2. 9 4 5 12PP12 System Functionality Access 9 4 5 13PP13 System Operational Qualification Spectrophotometer Compliance to Specification a 10 4 6 GIOSSANY P 10 4 7 Approved Report Formats for System Acceptance 10 TESI PROGRESS SHEET T OF 2 uu i or er i e 11 TEST PROGRESS SHEET 2 0E 2 5 eite Q ttt e ena amen abst 13 Page 2 of 14 UV Visible System Validator Issue 5 June 2003 NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN 1 Introduction This document provides all the essential information required to perform the correct installation to a basic operating level of a Nicolet Evolution 500 spectrophotometer with Local Control software This is reproduced in the Regulatory environment as Installation Qualification IQ and Operation Qualification OQ of the specified product The Validator 5 Log Book contains full instructions for Installation Operational Qualification of your Thermo Electron UV Visible spectrophotometer system It is available as a purchased option and can be ordered from your local Thermo Electron authorized Sales Support organization Part Number 10 03 0101 This IQ OQ procedure together with the completed Test Progress Sheet should be removed from this manual and filed for future reference in your Valid
3. 8 SETUP Page ce otim t P edet ha denen ch ERE RA 9 PRINTERS Page uw eor ee teli 11 ENVIRONMENT x itii tpa e eie editi eiue 12 WAVELENGTH CALIBRATION Page ierosin eiiean aaa nennen nnns nnns nnne ens 16 OPTIGCALEINIFIAEISATION Pa99O 2 ree eite tite diet a sees 17 EAMPS PA98 Su ua ag ie 18 SCAN mE 20 SCAN PARAMETERS Page iiic 21 SCAN GRAPH Page eo edet dr editi edet epe eon 25 each eden dora ekle et ee dv Pee 26 FUMED EE 32 tuia S Eesti itunes ire 32 Sha bhi 33 EIXED RESUETS Pa98 oie pa ie 35 QUANT n hen ren eL de eit cr a evt eet et 38 QUANT page oft in e etl kawak et de eiat 38 QUANI STANDARDS Page iire cte e Ee d egeo Wein te 41 QUANT C CALIBRATION ici rtt tet tn tee Fl ER qatapas saam aqa reae 42 QUANT RESULETS PAage atto d oder bte tes etti a eg er on 43 RAE hte tb Ae et Bot aan he il tae Naa ae ay het iru Mere iE 44 eatur 45
4. list shows the positions and values of the peaks as calculated by the function selected in MANIPULATE PEAKS The measured values will be ABS INTENSITY or 1D 2D 3D 4D depending on the current mode and are sorted by wavelength Each marker is identified as a peak valley or zero crossing LIMS EXPORT Sends the results via the RS232 port PRINT LIST Prints the list on the selected printer SCAN GRAPH Returns to the SCAN GRAPH page 29 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual RATIO TABLE Page RATIO TABLE The page shows the positions and values of the V a LE wavelengths and the ratio as selected by the 2 2002 463 0 RATIO or CORR RATIO functions 0 375 480 0 VIEW GRAPH Returns to the SCAN GRAPH page LIMS EXPORT Sends the results via the RS232 port PRINT LIST Prints the list on the selected printer SCAN GRAPH Returns to the SCAN GRAPH page PEAK HEIGHT Page PEAK HEIGHT a MARK ABS A WAVELENGTH nm This page shows the position and values of the wavelengths and the peak height as selected by 3 2245 275 the PEAK HEIGHT function 3 0 375 480 0 VIEW GRAPH Returns to the SCAN GRAPH page LIMS EXPORT Sends results via the RS232 port PRINT LIST Prints the list on the selected printer SCAN GRAPH Returns to the SCAN GRAPH page 30 Issue 1 August 2003 Nicolet Evolution 500 Local Control Softwar
5. 5 3 2 Product Documentation 2 2 ette esce ete atiende dte leprae itat 5 3 3 Product Development amp 5 3 4 Qualification User 5 4 System Acceptance Test 5 451 Introduction 5 4 2 SCOPC RID 5 4 3 Overview and Test Plan 5 4 4 Testing Requirements 4 doe e ode ede dde e e ata ute a ud A cute 6 4 5 Test Procedures uere Rc erect tienes de obse E 6 4 5 1 PP1 Initial Power aee eiea ennt herr 6 4 5 2 2 System Initialization sasirnane kerkii edar asrin iei i 6 4 5 3 PP3 System Functionality 6 4 5 4 PP4 System Functionality 7 4 5 5 5 System Functionality Data Store and 7 4 5 6 PP6 Data Transfer to Other 8 4 5 7 PP7 System Functionality Fixed eene 8 4 5 8 PP8 System Functionality Quantitation sse 8 4 5 9 PP9 System Functionality 8 4 5 10PP10 System Functionality Multicomponent Analysis 8 4 5 11PP11 System Functionality UVCalc Bio
6. 5 MINIMUM 0 0000 MAXIMUM 9999 9000 STD9 STD10 STD20 Calibrate method 19 01 98 QUANT GRAPH 0 281A 11 35 ID 550 0nm CANCEL ACCEPT 3 000 ABS 2 000 1 000 E UANT RESULTS page 0 300 j000 50 000 100 00 i Q pag Place std 1 1 in beam and press run STOP 19 01 98 0 281A 11 35 QUANT RESULTS 550 0nm COEFFICIENT 0 5670 Press RUN ABS CONC g ml 19 01 98 QUANT GRAPH DN 11 35 ID UV123 550 0nm 1 0 281 2 8100 3 000 2 0 281 2 8100 3 0 281 2 8100 ABS 4 0 281 2 8100 2 000 1 000 LIMS PRINT SAVE QUANT CLEAR EXPORT RESULTS DATA PAGE RESULTS 0 300 I 1 0 0000 50 000 g ml 100 00 COEFFICIENT 0 9999 Press Run to run SAVE PRINT STAND QUANT VIEW METHOD GRAPH ARDS PAGE RESULTS samples OX 1 472X 0 425 19 01 98 11 35 STANDARDS STD CONC ABSORBANCE 1 0 2500 0 1299 0 1299 2 0 5000 0 2179 0 2179 3 0 7500 0 4904 0 4904 4 1 0000 0 9312 0 9312 5 1 2500 1 4835 1 4335 6 1 5000 1 9332 1 9332 COEFFICIENT 0 9725 EQUATION EDIT EDIT QUANT VIEW CURVE STD PAGE RESULTS STANDARDS page calibrated 38 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Instrument and analysis parameters are set up on the QUANT page Move the cursor to the required parameter using the Up Down Arrow keys Change the parameter by pressing the ENTER key Q On
7. 4 December 2000 Version 6 53 7 December 2000 Version 5 56 17 September 2001 Version 7 00 23 July 2003 Initial release of the software Rate functionality added Service release Higher Order derivative functionality added to Scan Service release Support for UV3 and UV4 added Service release French and Spanish languages added German language added Service release Support for Gilson XL Autosamplers added Service release Service release Service release Service release Service release Service release UVCalc advanced results calculation added Service release Support for new printers added Support for UV300 500 Internal mercury lamp support enhancements to Quant Serial Fixed MCA analyses Service release Service release Service release Service release Service release Correction of Action Requests received since the release of V4 20 Service release Service release Service release Service release Service release Service release Modifications to user log on Support for Evolution 500 Correction of Action Requests received since the release of V6 56 NOTE WHILST EVERY EFFORT IS MADE TO ENSURE THAT THIS RELEASE HISTORY IS CURRENT THERE WILL BE TIMES WHEN CHRONOLOGICALLY THIS LIST MAY NOT REFLECT THE LATEST RELEASE S OF SOFTWARE IN THESE CIRCUMSTANCES AN AMMENDMENT TO THIS TEXT WILL BE AVAILABLE ON REQUEST 3 Technical Description The Local Control software pro
8. i 0 200 i i i i i i I 0 000 27 00 00 00 15 00 30 RATE CLEAR VIEW FAST GRAPH ALL RESULTS RATE 19 01 98 RATE GRAPH 0 281A 11 35 ID TEST 1 550 0nm 0 400 ABS 0 200 0 000 1 00 00 00 15 00 30 MANIP PRINT SAVE RATE VIEW ULATE DATA PAGE RESULTS 19 01 98 RATE RESULTS 0 281A ID TEST 1 E Initial Abs 1 281 Final abs 0 567 Initial Time 00 00 FinalTime 00 30 AA Min 1 281 Activity 4281 Corr Coeff 0 987 Smoothing LOW LIMS SAVE RATE VIEW PRINT EXPORT DATA PAGE GRAPH 19 01 98 RATE RESULTS 0 281A 11 35 ID TEST 1 550 0nm 1 2 3 4 Initial Abs 1 281 1 281 1 281 1 281 Final abs 0 567 0 567 0 567 0567 Initial Time 00 00 00 00 00 00 00 00 Final Time 00 30 00 30 00 30 00 30 AA Min 1 281 1 281 1 281 1 281 Activity 4281 4281 4281 4281 Corr Coeff 0 987 0 987 0 987 0 987 Smoothing LOW LIMS SAVE RATE VIEW EXPORT RRINT DATA PAGE GRAPH 44 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Toselect Rate highlight the RATE option on the HOME PAGE and press ENTER The RATE Methods page is displayed and from here the instrument and analysis parameters can be set up Move the cursor to the required parameter using the Up Down Arrow keys Press ENTER to enable a parameter to be changed Once the method has been set up
9. DEFAULT FILE TYPE NORMAL CVU WAVELENGTH TEST FILTER LIMS SUPPORT OFF USE SAMPLE IDS OFF AUTOSAVE RESULTS OFF AUTOPRINT RESULTS OFF USER LOG ON OFF HISTORY FILE OFF J LANGUAGE Select from the list The language used immediately changes to the one selected SOUND Turns the warning beeper ON or OFF If set to OFF then the only indication of any error is the screen message DATE FORMAT Defaults to dd MM yy but will toggle with ENTER to MM dd yy AUTOMATIC CAL VAL Toggles between OFF and ON with Enter When ON and the CVU Calibration Validation Unit is fitted the instrument automatically waits on start up for the warm up period 60 minutes and then performs the Wavelength Absorbance and Stray Light calibration tests See CVU Section Pressing Clear aborts the calibration DEFAULT FILE TYPE Selects the default file type on the SAVE RENAME page Available formats are NORMAL The native file type used by the Local Control software CSV Comma separated variable JCAMP DX JCAMP data exchange format Use up down arrow keys to highlight choice and press ENTER to confirm selection CVU WAVELENGTH TEST Toggles between Filter or Hg Selects the method used by the CVU where fitted to perform the Wavelength Accuracy Test 12 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual LIMS SUPPORT Toggles between OFF and ON
10. Evolution 500 Local Control Software Manual Note This manual is being revised so some of the information you will find in it is out of date Please accept our apologies for any confusion this may cause Any reference to Unicam Spectronic Instruments or Thermo Spectronic has changed to Thermo Electron Corporation and the contact and trademark information has also changed On the next page you will find a current disclaimer and up to date contact and trademark information Please contact Thermo Electron if you have any questions or concerns P N 10 50 0311 Thermo ELECTRON CORPORATION The information in this publication is provided for reference only information contained in this publication is believed to be correct and complete Thermo Electron Corporation shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing performance or use of this material All product specifications as well as the information contained in this publication are subject to change without notice This publication may contain or reference information and products protected by copyrights or patents and does not convey any license under our patent rights nor the rights of others We do not assume any liability arising out of any infringements of patents or other rights of third parties We make no warranty of any kind with regard to this material including but not lim
11. Q When MEASURE STDS YES Use the up and down arrow keys to move up and down the list When the highlight is on the field to be entered or edited press ENTER to display the Text Entry dialogue box Enter the name of the Standard and press the ACCEPT function key when finished The instrument returns to the STANDARDS ENTRY page with the concentration field for the standard highlighted Press ENTER to display the edit dialogue box enter the concentration using the numeric keypad and press ENTER when finished The instrument returns to the STANDARDS ENTRY page with the ID for the next standard highlighted Up to 20 standards can be entered in this way The standards are displayed on two pages The STD11 STD20 function key toggles between the two pages When MEASURE STDS NO Press ENTER to display the LIBRARY page Select and load the files for each standard in turn A library of standards can be built up using the MULTI function in FIXED The same method must be used for each MULTI result and will be used for the MCA analysis When the first MULTI A file is loaded the current MCA method is changed to that used to obtain the MULTI A result Standards can thus be used in any combination without having to recalibrate for each new mixture STD1 STD10 STD11 STD20 Toggles between displaying the first and second halves of the Standards List ACCEPT transfers to the MCA METHODS page with the new list accepted CANCEL
12. June 2003 NICOLET EVOLUTION 500 2 1 LOCAL CONTROL IQ OQ PLAN Development Release History Nicolet Evolution 500 Local Control software is a development of Thermo Electron s well established UV Visible Local Control Software while building on the firm foundation of its predecessor it also incorporates significant enhancements Local Control Software has a well demonstrated history of problem free usage in the laboratory Customer experience has demonstrated consistent conformance to software and system requirements When used as specified there have been no reports of safety problems Version 1 00 25 July 1993 Version 2 00 20 October 1993 Version 2 02 16 November 1993 Version 2 04 3 December 1993 Version 3 00 4 February 1994 Version 3 10 23 February 1994 Version 3 31 25 March 1994 Version 3 32 20 April 1994 Version 3 50 31 August 1994 Version 3 51 25 October 1994 Version 4 00 22 March 1995 Version 4 11 15 November 1995 Version 4 15 28 February 1996 Version 4 21 6 June 1997 Version 4 24 9 September 1997 Version 4 30 10 November 1997 Version 5 00 21 April 1998 Version 5 01 29 April 1998 Version 5 02 6 May 1998 Version 5 03 19 May 1998 Version 5 04 20 May 1998 Version 5 05 5 August 1998 Version 5 06 23 December 1998 Version 6 04 24 December 1999 Version 6 06 11 January 2000 Version 6 07 18 January 2000 Version 6 51
13. Transfers to the MCA METHODS page leaving the old list unchanged 55 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual WAVELENGTH ENTRY page Use the up and down arrow arrow keys to move up and down the list When the highlight is on the field to be entered or edited press ENTER to display the EDIT box and enter the values with the numeric keys It is not necessary to enter the values in numerical order although analysis will be quicker if they are Press ENTER when finished The instrument will return to the WAVELENGTH ENTRY page with the new value inserted in the list and the highlight moved to the next wavelength When all wavelengths have been entered press the ACCEPT function key to return to the MCA methods page using the new list or the CANCEL function key to return leaving the old list unchanged Alternatively wavelengths may be entered directly from a scan Clear the beam s and press ZERO BASE to perform a baseline scan then put the sample in the cell holder and press the SCAN GRAPH function key The instrument performs a scan using the method currently entered in the SCAN PARAMETERS page Use left and right arrow keys to move the vertical cursor to a suitable wavelength and press ENTER to mark it Repeat until all required wavelengths have been marked Marks can be removed using the CLEAR key or the CLEAR ALL function key When all required wavelengths have been marked press the ACCEPT function key to accep
14. calling page The original entry is not changed 6 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual SAVE RENAME This page is used for saving or renaming files from any part of the LOCAL CONTROL software The page is used to name a file and or change the ID coming to the page the Filename field is ready for editing Use the Arrow keys to move the cursor to the required character and press ENTER Once all the required characters have been entered press ACCEPT Numbers are entered using the numeric keypad If you make a mistake will act as backspace or CLEAR will clear the whole entry Filenames are limited to 8 characters Tochange the FILE TYPE highlight the field and press ENTER to display the pop up menu Available formats are NORMAL the native file type of the Local Control Software CSV Comma separated variable and JCAMP DX JCAMP data exchange format Highlight your choice and press ENTER to select The ID field will contain the characters entered for the method To edit the ID highlight the ID field and press ENTER change as necessary then press ACCEPT To select the destination of the file highlight the DRIVE field Pressing ENTER will toggle between LIBRARY and DISK SAVE lt Acts as backspace and clears the last character in the line ACCEPT SAVE Accepts the edited field ready for saving Only
15. en ae eat a 47 RATE RESULETS Pa96 entere ripam eate 50 Parallel Rate Measurements using the Cell 51 M Sabe 52 STANDARDS ENTRY Page eei quoa aei 55 WAVELENGTH ENTRY page eunte eid et eri o quce to e T e edens 56 bL 57 Analysing a Sampl u rece iret tt art ne i Rire ned tL ie e ea ne End 57 UV Cal cm PE 58 Introduction iae e u Se a h Peto er oe 58 Specification 58 Serum UE 59 UN Calc HOW tO iato eere te eaten e certi p ae e t Tee ute esit ite eee 60 UVCGalc error messages editt deed ee de ind ela Rode Eein deua 64 1 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual This page is intentionally left blank 2 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual GENERAL Introduction The Nicolet Evolution 500 UV Visible spectrophotometer can be controlled independently via an integral keypad and LCD display or externally from a computer The Evolution 500 is available in both local control and blind bench variants This manual describes the operation of the Local Control software on the Evolution 500 spectrophotometers The syste
16. SCAN VIEW MANIPULATE ULATE GRAPH DATA PAGE RESULTS TRACK RESCALE mone n Choose Track to report NE wavelength values onremar 19 01 98 TRACK 0 281A 0 00 11 35 ID TEST 1 550 0nm 400 0 450 0 500 0 550 0 600 0 MANIP PRINT SAVE SCAN VIEW 2 000 ULATE GRAPH DATA PAGE RESULTS ABS 5 Choose Peaks to give 2 Peak Table rid V 19 01 98 TRACK LIST 0 281A T 11 35 reer 550 0nm N J L 0 000 e inn MARK ABS A WAVELENGTH nm 400 0 450 0 500 0 850 0 600 0 SCAN PRINT CLEAR VIEW 1 0 472 447 0 GRAPH GRAPH ALL FAST TABLE 2 2 002 463 0 3 0 375 480 0 4 0 406 526 0 1 ped 456 Choose View Table to o eee see Track Table SCAN PRINT LIMS VIEW TRACK TABLE page GRAPH LIST EXPORT GRAPH 19 01 98 PEAK TABLE 0 281A 11 35 ID TEST 1 550 0nm ABS A WAVELENGTH nm 1 PEAK 0 472 447 0 2 PEAK 2 002 463 0 3 PEAK 0 375 480 0 4 PEAK 0 406 526 0 PEAK TABLE page 5 PEAK 2 020 543 0 6 PEAK 0 441 559 0 SCAN PRINT LIMS Choose View results to see Peak Table GRAPH LIST EXPORT Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Toselect Scan highlight the SCAN option on the HOME PAGE and press ENTER The SCAN Methods page is displayed and from here the instrument and analysis parameters can be set up Move the cursor to the required parameter using the Up Down Arrow keys Press ENTER to enable a parameter to be changed Once the method
17. Use the up and down arrow keys to move the highlight to USER LOG ON and press Enter This brings up the Text Entry Page When the correct password is entered USER LOG ON is set to ON otherwise an error message is displayed and it remains set to OFF The default password is ADMIN Note that the password is case sensitive and in this password all the letters are upper case Logging on as ADMIN gives access to the system at Administrator level and the CHANGE USERS function key is enabled CURRENT USERS NAME PASSWORD PRIVILEGES ECFIHLB ADMIN ADMIN ANN NANN EDIT METHODS H HISTORY FILE C CALIBRATIONS L RESET LIFETIMES F DELETE FILES B DEFAULT BASELINE I INITIALISATIONS USERS CANCEL ACCEPT 11 35 Pressing the CHANGE USERS function key brings up the CURRENT USERS page Up to 20 users can be listed by name and password The privileges of each user can be set individually by the Administrator and can be any combination of Edit Methods Calibrations Delete Files Initialisations History File Reset Lifetimes Default Baseline Only the Administrator is able to change passwords or edit the Current Users Page It is strongly recommended that a new user name and password for the Administrator are set as soon as USER LOG ON is activated and that USER LOG ON is activated whenever the instrument is to be used in a multi user environment After USER LOG ON is enabled each time the instrument is powered up the
18. accept the new list or press CANCEL to return to the FIXED METHOD page leaving the original list unchanged The available wavelength range is 190 0nm to 900 0nm If the wavelength requires the Deuterium lamp then this will be switched on The current data will be lost if the wavelength is changed BANDWIDTH Select from 4 2 1 5 1 0 5 and 0 2nm INTEGRATION Enter integration time in seconds This sets the integration time for which the result is measured The current data will be lost if the integration time is changed DELAY TIME Set a time in the range 00 05 to 99 59 using to separate minutes and seconds This sets the time between pressing RUN and the start of the measurement The range is from 0 to 99 minutes and 59 seconds LAMP CHANGE Select from 315 320 325 330 335 340 D2 W Hg Q Selects the wavelength at which the source is changed between the Tungsten and Deuterium lamps Selecting D2 W or Hg overrides any changeover and the selected lamp will be used regardless of the wavelength set THE MERCURY LAMP MUST BE SWITCHED OFF WHENEVER IT IS NOT IN USE OPERATOR Switches to the TEXT ENTRY screen operator name is automatically saved with the method any data produced by the method Changing the operator name will not cause any current data to be lost If user log on is in operation the operator name cannot be changed UVCALC Switches to the UVCALC screen CELL PROG Switches to t
19. and 59 seconds This field is only available if UVCalc has been installed LAMP CHANGE Select from 315 320 325 330 335 340 D2 W Hg Selects the wavelength at which the source is changed between the Tungsten Deuterium lamps Selecting D2 W or Hg overrides any changeover and the selected lamp will be used regardless of the wavelength set THE MERCURY LAMP MUST BE SWITCHED OFF WHENEVER IT IS NOT IN USE The current data will be lost if the lamp change parameters are changed OPERATOR Switches to the TEXT ENTRY screen operator name is automatically saved with the method and any data produced by the method Changing the operator name will not cause any current data to be lost If User Log on is in operation the operator name cannot be changed CELL PROG Switches to the CELL PROG screen if fitted REF MODE Toggles the status of the Cell Programmer Reference Mode if fitted VIEW RESULTS Transfers to the Results Table if it contains results VIEW STDS Transfers to Standards results table if a calibration has been performed SAVE METHOD Brings up the SAVE page from where the method can be saved to Disk PRINT METHOD Prints the current method parameters on the printer CALIBRATE Moves to the CALIBRATION PAGE to perform a calibration if standards and wavelengths have been entered 54 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual STANDARDS ENTRY Page
20. current data to be lost REPLICATES Enter number of replicates for each standard Sets the number of times each standard is measured maximum 3 Each value obtained is used in the calibration 39 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual UNITS Enter units for concentration using the TEXT ENTRY page CURVE FIT Select from LINEAR LINEAR TO 0 QUADRATIC QUAD TO 0 Q Selects the curve fit algorithm used in the calibration LINEAR performs a linear calibration At least two standards are required LINEAR TO 0 performs a linear calibration forced through zero At least one standard is required QUADRATIC performs a quadratic fit on the data At least three standards are required QUAD TO 0 performs a quadratic fit with the data forced through zero At least two standards are required Changing the curve fit will cause the existing calibration to be recalculated Any results associated with the previous calibration will be lost LAMP CHANGE Select from 315 320 325 330 335 340 D2 W Hg Selects the wavelength at which the source is changed between the Tungsten and Deuterium lamps Selecting D2 W or Hg overrides any changeover and the selected lamp will be used regardless of the wavelength set THE MERCURY LAMP MUST BE SWITCHED OFF WHENEVER IT IS NOT IN USE Any current data will be lost if the lamp changeover wavelength is changed OPERATOR Switches to the TEXT ENTRY screen The
21. has been set up press ZERO BASE to perform a baseline scan with the current method see page 4 and then press RUN The spectrophotometer will perform the scan and display the result on the SCAN GRAPH page From here the spectrum can be manipulated and saved to Library or Disk See LIBRARY for explanation of file functions SCAN PARAMETERS Page Note The current spectrum will be lost if any of the method parameters except for the Operator name are changed SCAN 1 SCAN TYPE INTELLISCAN ID TEST 1 MODE ABS START 400 0 nm STOP 600 0 nm BANDWIDTH 2 0 nm SPEED NORMAL DATA INTERVAL 1 0 nm PEAK TABLE OFF GRAPH HIGH 2 000 GRAPH LOW 0 000 I LAMP CHANGE 325 nm I I OPERATOR Name 1 UVCALC 0 CELL PROG AUTO REF __ SCAN TYPE Select from STANDARD INTELLISCAN Standard mode enables the setting of a given scan speed and data interval Intelliscan mode sets the data interval automatically and varies the scan speed according to the Absorbance of the spectrum ID 5 Enter a description using the TEXT ENTRY screen ThelD identifies the method and will be saved with the method and any spectra produced by the method 21 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual MODE Select from ABS T l 1D 2D 3D 4D ABS Selects Absorbance Selects 96 transmittance Selects Intensity m
22. operator name is automatically saved with the method and any data produced by the method Changing the operator name will not cause any current data to be lost If User Log on is in operation the operator name cannot be changed UVCALC Switches to the UVCALC screen MEASURE STDS Toggles between YES and NO When YES and Standard concentrations have been entered from the Quant Standards page pressing Calibrate causes the instrument to prompt the user to put the standard in the beam and press Run to measure for each Standard in turn When NO pressing Calibrate causes the system to prompt for an absorbance to be entered manually for each Standard effectively enabling a calibration originating elsewhere to be entered CELL PROG Switches to the CELL PROG screen if fitted REF MODE Toggles the status of the Cell Programmer Reference Mode if fitted 40 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual QUANT Page function keys QUANT VIEW CALIB Switches to the QUANT GRAPH page if a valid calibration exists VIEW RESULTS Switches to the Quant Results page if there are any sample results SAVE METHOD Brings up the SAVE page from where the method can be saved to Library or Disk PRINT METHOD Prints the current method parameters and the standards table on the printer CALIBRATE Switches to the Standards Entry page QUANT STANDARDS Page a This page l
23. press RUN The spectrophotometer will perform the rate and display the result on the RATE GRAPH page From here the data can be manipulated and saved to Disk RATE PARAMETERS Page Note The current data will be lost if any of the method parameters except for the ID Slope Factor Units and Operator name are changed RATE D i ID TEST 1 WAVELENGTH 840 0 nm BANDWIDTH 2 0 nm MEASURE TIME 00 30 DELAY TIME 00 00 SLOPE POSITIVE RANGE 0 5 i FACTOR 1 000 UNITS I U LAMP CHANGE 325 nm OPERATOR Name CELL PROG AUTO REF MODE 2 OFF ID Enter a description using the TEXT ENTRY screen ThelD identifies the method and will be saved with the method and any results produced by the method WAVELENGTH The available wavelength range is 190 0nm to 900 0nm If the wavelength requires the Deuterium lamp then this will be switched on The current data will be lost if the wavelength is changed BANDWIDTH Select from 4 2 1 5 1 0 5 and 0 2nm MEASURE TIME Set a time in the range 00 05 to 99 59 This sets the time over which the sample will be measured The range is from 5 seconds to 99 minutes and 59 seconds in steps of 1 second Ifthe cell programmer is ON then the measurement time sets the time between individual measurements on the first cell i e the time for each cycle DELAY TIME Set a time in the range 00 05 to 99 59 This sets the time between
24. the TEXT ENTRY screen ThelD identifies the method and will be saved with the method and any results produced by the method SELECT Select from SINGLE MULTI SERIAL SINGLE This option is used to measure each sample at a single wavelength which is the same for each sample MULTI This option allows each sample to be measured at up to 20 wavelengths which are the same for each sample SERIAL This option allows a single wavelength measurement to be made at a different wavelength on up to 9 samples 33 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual WAVELENGTH S SINGLE Use the numeric key pad to enter the required wavelength into the pop up box Press ENTER when finished MULTI Use the up and down arrow keys to move to the wavelength to be entered or edited and press ENTER to display the edit box Use the numeric keypad to enter the wavelength and press ENTER when finished The instrument returns to the MULTI screen with the next wavelength in the list highlighted Up to 20 wavelengths may be entered When the list is finished press the ACCEPT function key to accept the new list or the CANCEL function key to return to the FIXED METHOD page without changing the wavelength list SERIAL Press ENTER to display the edit box for the wavelength to be used for the first sample Data entry is as for MULTI above When the required wavelengths have been entered press ACCEPT to
25. this function allows the results from any cell in the run to be selected and displayed Enter the number of cells you wish to display RATE RESULTS Page The Rate Results page displays the Initial and Final Absorbance Initial and Final Time the change in absorbance per minute calculated Activity Correlation Coefficient of the best fit line and finally the smoothing parameter used If the rate curve has been tracked the Initial and Final Absorbance with the Initial and Final Time will reflect those chosen by the two cursors Shown below is the Rate Results page after choosing SECTION and the three sets of data represent results calculated for each section A similar display will be seen with TRACK RATE RESULTS 1 2 3 Initial Abs 1 281 0 992 0 662 Final Abs 0 992 0 662 0 512 Initial Time 00 00 00 10 00 40 Final Time 00 10 00 40 01 00 A A Min 1 734 0 660 0 450 Activity 4281 1630 1111 Corr Coeff 0 987 0 995 0 952 Smoothing LOW LOW LOW Clll VIEW GRAPH Returns to the RATE GRAPH page RATE PAGE Returns to the RATE PARAMETERS page SAVE DATA Brings up the SAVE screen from where method and data can be saved to disk PRINT Provides a hardcopy of the results i e RATE GRAPH and RATE RESULTS LIMS EXPORT Sends the results via the RS232 port 50 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Parallel Rate Measurements using th
26. 03 Nicolet Evolution 500 Local Control Software Manual FIXED RESULTS ABS ABS 1 0 201 15 2 0 201 16 CLEAR RESULTS All results are cleared ready for the start of the next batch FIXED PAGE Returns to the FIXED METHOD page SAVE DATA Brings up the SAVE page from where the results can be saved to Disk PRINT LIST Prints the list on the selected printer LIMS EXPORT Sends the results via the RS232 port Press RUN to take another sample measurement Press ZERO BASE to zero the instrument at the wavelength s specified in the method 36 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual This page has intentionally been left blank 37 Issue 1 August 2003 QUANT page Nicolet Evolution 500 Local Control Software Manual QUANT 19 01 98 0 281A 550 0nm 14 QUANT am Set parameters as required ID WAVELENGTH 550 0nm BANDWIDTH 2 0nm BANDWIDTH 0 224 STANDARDS ENTRY page STANDARDS 3 i REPLICATES 1 19 01 98 OGHiA UNITS 11 35 550 0nm CURVE FIT LINEAR STANDARDS LAMP CHANGE 325nm OPERATOR Name lt nTP s 0 2500 STD11 UVCALC 0 STD2 VALUE MEASURE STDS YES es STD3 CELL PROG AUTO Fill in standards STD4 CONC 053500 STD5 ac CALIB PRINT SAVE VIEW VIEW STD6 RATE METHOD METHOD RESULTS
27. 586 23 A5 6 27 8 29 10 Press CALIBRATE 1 A 12 213 14 15 16 X17 18 219 A20 CANCEL ACCEPT SCAN 09 03 98 0 281A 11 35 550 0 Didymium ABS ABS 241 0 206 22 1 605 When all components have been calibrated 09 03 98 0 281 11 35 550 0nm insert first sample and press RUN ABS ABS 1 Al 2 036 22 1 646 AV YB n COMPONENT CONC COMPONENT CONC STOP Holmium 0 9983 Didymium 0 9903 GOODNESS OF FIT 0 0000 PRINT SAVE MCA CLEAR LIST DATA PAGE RESULTS 52 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual The MCA Multi Component Analysis application is able to measure up to 20 components in a mixture measuring up to 20 wavelengths per sample Standards be measured at run time or loaded from files obtained using the MULTI function of FIXED MCA METHOD Page MCA 1 ID TEST 1 MEASURE STDS YES STANDARDS 0 UNITS WAVELENGTH S 1 BANDWIDTH 2 0nm INTEGRATION 15 DELAY TIME 00 LAMP CHANGE 325nm OPERATOR Name CELL PROG AUTO LREF MODE _ t lt 1 oc 1 ID Enter a description using the TEXT ENTRY screen The ID identifies the method and will be saved with the method and any spectra produced by the method MEASURE STDS Use ENTER to toggle between YES and NO YES Standards are measured with the run and all fields remain editable NO Standards
28. ABLE and press ENTER then select PEAK HEIGHT A pop up box appears in which to enter the wavelengths and define the baseline A defines the peak Once all the parameters have been set save the method GRAPH HIGH Select from range GRAPH LOW 0 01 to 6 00 Sets the upper graph limits on the SCAN GRAPH page GRAPH HIGH must be 0 01 greater than GRAPH LOW GRAPH LOW Select from range 0 3 to GRAPH HIGH 0 01 Sets the lower graph limits on the SCAN GRAPH page GRAPH LOW must be 0 01 less than GRAPH HIGH LAMP CHANGE Select from 315 320 325 330 335 340 D2 W Hg Selects the wavelength at which the source is changed between the Tungsten and Deuterium lamps Selecting D2 W or Hg overrides any changeover and the selected lamp will be used regardless of the wavelength set THE MERCURY LAMP MUST BE SWITCHED OFF WHENEVER IT IS NOT IN USE OPERATOR Switches to the TEXT ENTRY screen The operator name is automatically saved with the method and any spectra produced by the method Changing the operator name will not cause the current spectrum to be lost If User Log on is in operation the operator name cannot be changed UVCALC Switches to the UVCALC screen CELL PROG Appears when the Cell Programmer is fitted Switches to the CELL PROG screen REF MODE Appears when the Cell Programmer is fitted Toggles the status of the Cell Programmer reference mode 24 Issue 1 August 2003 Nicolet Evolution 500
29. D CELL PROGRAMMER MODE An invalid cell programmer mode has been selected Check settings FORMULA CONTAINS OUT OF RANGE WAVELENGTH You have selected wavelengths outside the range set for the scan 64 Issue 1 August 2003 ALIDAIOR Nicolet Evolution 500 Local Control IQ OQ Plan Copyright 2003 Thermo Electron Corporation All rights reserved Europe USA Mercers Row 5225 Verona Road Cambridge CB5 8HY UK Madison WI 53711 USA Tel 44 0 1223 446600 Tel 1 800 201 8132 or 1 608 276 6100 Fax 44 0 1223 446644 Fax 1 608 273 5046 E mail spectroscopyuk thermo com E mail spectroscopy thermo com www thermo com spectronic www thermo com spectronic Thermo ELECTRON CORPORATION NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN Contents et epe endo ets sa ass eee ot dudas item 3 1 15 JdentifiCatiOn c eec tle ete Saa E M T dnd leen 3 1 2 Manufacturer u u ku A ieee ode a dente ot de Pado 3 1 3 General Deserlptlon a ote ROSE ERES 3 1 4 irn e E re HR e P der t ye ide 3 2 Product iti i en tet dete ta etiem es 3 2 1 Development Release 4 3 Technical Description netto e ee ERR RASEN Si H Ee RR AIR a Ee 4 3 1 Thermo Electron Accessories Directly Supported
30. ER CELL The cell number currently displayed is shown to the right of the ID line To print the results press PRINT on either the RATE GRAPH page or the RATE RESULTS page Three further print options are available ALL OVERLAY ALL SEQUENTIAL and ONE RESULT ALL OVERLAY Prints the results of all cells in the run together in batches of 4 TRACK and SECTION markers are not included ALL SEQUENTIAL Prints each result in the run separately TRACK and SECTION markers are included ONE RESULT Prints the result currently displayed TRACK and SECTION markers are included 51 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual MCA 09 03 98 0 281A 11 35 MCA 550 0nm tit STD ID CONC Enter identities and zn 19 01 98 0 281A 11 35 MCA 550 0nm concentrations of 1 HOLMIUM 2 DIDYMIUM standards 3 ridus ID TEST 1 4 MEASURE STDS YES 5 STANDARDS 10 te 6 y UNITS 7 8 WAVELENGTH S 1 a V F 9 BANDWIDTH 2 0nm 10 2 INTEGRATION 118 DELAY TIME 00 00 STD10 LAMP CHANGE 325nm CANCEL ACCEPT STD20 OPERATOR Name CELL PROG AUTO REF MODE OFF CALIB PRINT SAVE VIEW RATE METHOD METHOD STDS RESULTS 09 03 98 0 281A 11 35 WAVELENGTH S 550 0nm Enter wavelengths to be used Ame A A1 446 A2
31. LE FACTOR Highlight Fixed and press ENTER Enter a suitable ID for the factor and press ACCEPT Highlight 4 and press ENTER Highlight F and press ENTER This time select Variable Factor from the pop up Enter a suitable ID for the factor and press ACCEPT Highlight press ENTER Press ACCEPT to return to the main uvcalc page Press ACCEPT twice more to return to the FIXED page Insert sample and press RUN You will be prompted when to input the factor values 62 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual How to modify an equation e g to modify the initial equation to M2 M1 50 0 Where M1 becomes a once only constant and M2 becomes measure with each run On the FIXED page go to the uvcalc line and press ENTER Go to EQUATION 1 and press ENTER The equation prepared in the example above should be displayed Go to FORMULA and press ENTER The cursor should be at the end of the existing formula Press SWITCH FIELDS and navigate the cursor to 1 Press ENTER Alter selection to ONCE ONLY Press SWITCH FIELDS Enter the M2 and For 2 select Measure with each run Press SWITCH FIELDS and move the cursor to the Press SWITCH FIELDS again and add the closing bracket Press ACCEPT This will return you to the main uvcalc page Make appropriate entries for the TITLE and UNITS line Press ACCEPT twice This will return you to the FIXED page 63 Iss
32. Local Control Software Manual SCAN PARAMETERS page function keys SCAN VIEW RESULTS Switches to the Scan Peak Table screen if any of the peak functions have been performed or the Track Table screen if track has been used VIEW GRAPH Switches to the Scan Graph screen SAVE METHOD Brings up the Filename Function screen and then saves the method including ID operator name and track wavelengths if the PEAK TABLE parameter is set to TRACK PRINT METHOD Prints the current method parameters on the printer UVCALC RESULTS Switches to UVCalc results screen if an equation has been entered and results are available mni SCAN GRAPH Page This page displays spectra and allows them to be manipulated 400 0 500 0 600 0 plolololo Y ____ VIEW RESULTS Switches to the PEAK TABLE page SCAN PAGE Returns to the SCAN page SAVE DATA Displays the SAVE screen from where method and data can be saved to disk PRINT GRAPH Provides a hardcopy of the results as shown on Screen MANIPULATE Displays the Manipulate options Pressing RUN starts a scan using the current method Q Pressing ZERO BASE starts a baseline using the current method 25 Issue 1 August 2003 MANIPULATE OPTIONS Nicolet Evolution 500 Local Control Software Manual MANIPULATE TRACK RESCALE COMPARE MODE PEAKS SMOOTHING ORIGINAL TRACK Reports x and y axis values using the tracking cursor RESCALE Cha
33. ME control of the LOCAL CONTROL software To enable control from an external computer press REMOTE on the HOME page Control will go to the computer providing the instrument O O O O i is idle REMOTE To return control to the Local Control software press the HOME key Control will revert to the Local Control software providing that the instrument is idle and the PC software has relinquished control TEXT ENTRY Page This page is used for entry of method operator and sample identities in all applications and for entry of concentration units in QUANT and FIXED methods The title displayed at the top of the page depends on which parameter is being edited On entry to this page the name field is filled with the current value OPERATOR Enter up to 11 characters for operator ID METHOD ID Enter up to 11 characters for method description UNITS Enter up to 11 characters for Conc units SAMPLE ID Enter up to 11 characters for sample ID Use the Arrow keys to move the cursor to the required character and press ENTER Once all the required characters have been entered press ACCEPT Numbers are entered using the numeric keypad If you make a mistake will act as backspace or CLEAR will clear the whole entry 8196190 lt Acts as backspace clears the last character the line ACCEPT Enters the names and returns to the calling screen CANCEL Abandons the naming operation and returns the
34. ON page CLOSE PRINTER Switches to the PRINTER page ENVIRONMENT Switches to the ENVIRONMENT page PRINTER WAVELENGTH Switches to the WAVELENGTH CALIBRATION page INUPIALISE INITIALISE Switches to the OPTICAL INITIALISATION WHITE LIGHT WHITE LIGHT Switches to the WHITE LIGHT Page To return to the HOME page press HOME CLOCK Page s 5 CLOCK From this page the internal spectrophotometer clock calendar can be reset TIME To reset the time or date highlight the required parameter and HOURS press ENTER Enter the new value using the number keys MINS 32 and press ENTER Q Once all the parameters have been changed press ACCEPT DATE DAY 25 The date or time will not be changed unless ACCEPT is pressed MONTH 12 YEAR 96 CANCEL cancels the edit leaving the previous values UU unchanged 9 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual EHT CALIBRATION Page __ EHT CALIBRATION EHT TABLE NORMAL EHT TABLE SETUP CALIB PAGE RATE Thispage isused to select and calibrate the table used table sets the amount of gain used at the photomultiplier detector ensuring that the response of the detector is kept optimal across the wavelength range If the EHT is set too low then the signal will be noisy conversely if the EHT is set
35. OPERATOR Name UVCALC 0 CELL PROG AUTO REF MODE OFF PRINT SAVE VIEW Set METHOD METHOD RESULTS ass 550 02 id z Onm FIXED RESULTS FIXED RESULTS WAVELENGTH nm ABS 1 2183 63 1290 35 11 550 0 ora 90 39 490 33 2 32 560 0 0 644 5 90 30 2 6 89 73 43 570 0 0 044 4 24 575 0 0 043 5 25 580 0 0 044 6 26 454 0 0 644 a 536 0 0 248 8 28 420 0 0 152 9 29 410 0 0 057 me rue mee Ln ENS TRUE T ET TEES EXPORT LIST DATA PAGE RESULTS Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Instrument and analysis parameters are set up on the FIXED page Move the cursor to the required parameter using the Up Down Arrow keys Change the parameter by pressing the ENTER key Q Once the method has been set up press ZERO BASE to zero the instrument for the current method See page 4 and press RUN The spectrophotometer will perform a measurement and display the result on the FIXED RESULTS page Q Once all results have been collected save the data FIXED METHOD Page FIXED 1 MODE ABS ID I SELECT SINGLE WAVELENGTH S 550 0 nm BANDWIDTH 2 0 nm INTEGRATION 1s DELAY TIME 00 00 LAMP CHANGE 825 nm OPERATOR UVCALC 0 CELL PROG AUTO REF 1 J MODE Select from ABS T ABS Selects Absorbance T Selects transmittance ID Enter a description using
36. Other Packages 1 Ensure the Application is set to Scan if not press Home Scan Enter 2 Export the data produced from PP4 System Functionality procedure Press Save Data enter PP5 or another suitable name for the filename change the file type to CSV ensure the Drive is set to Disk place a floppy disk in the disk drive and press Save 3 Using a PC open Windows NotePad and load the csv file Another program that will load csv file types may be used The successful loading of data into NotePad or another program that can load csv file types constitutes a pass to this test PP7 System Functionality Fixed 1 Ensure the Application is set to Fixed if not press Home Fixed Enter 2 Press the Run button New data in the Results screen constitutes a pass to this test PP8 System Functionality Quantitation 1 Ensure the Application is set to Quant if not press Home Quant Enter 2 Select Standards and press Enter Enter the values for 4 standards and press Accept Any standards may be used but the absorbance values should be about 0 5 1 0 1 5 and 2 0 A 3 Zero the spectrophotometer Press ZeroBase 4 Calibrate the method Press Calibrate and follow any prompts 5 Place a sample with an expected absorbance between 0 5 and 1 5 in the sample position 6 Press the Run button and follow any prompts New data on the Results screen and a calibration curve in the Quant Graph screen con
37. PROG screen if fitted REF MODE Toggles the status of the Cell Programmer reference mode if fitted RATE PARAMETERS Page function keys VIEW RESULTS Goes to the RATE RESULTS page VIEW GRAPH Goes to the RATE GRAPH page SAVE METHOD Brings up the SAVE page from where the method can be saved to Library or Disk PRINT METHOD Prints the current method parameters on the printer 46 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual RATE GRAPH Page Q This page displays the RATE curve and allows it to be manipulated VIEW RESULTS Switches to the RATE RESULTS page RATE PAGE Returns to the RATE PARAMETERS page SAVE DATA Brings up the SAVE screen from where method and data can be saved to disk PRINT Provides a hardcopy of the results i e RATE GRAPH and RATE RESULTS If a Cell Programmer is fitted see the note below MANIPULATE Brings up the Manipulate options than one rate has been run in parallel using the Cell Programmer then three further print options are available ALL OVERLAY ALL SEQUENTIAL and ONE RESULT ALL OVERLAY Prints the results of all cells in the run on one sheet of paper up to a maximum of 4 results If more than four results are present then the remainder are printed on a second sheet ALL SEQUENTIAL Prints each result in the run on a separate sheet of paper ONE RESULT Prints the result currently displayed P
38. This option gives a pop up menu for changing the graph y axis scale Move the cursor to one of the options and press ENTER to select an operation The rescale options depend on whether Absolute or Relative Absorbance has been selected Absolute Absorbance RESCALE AUTO GRAPH HIGH GRAPH LOW AUTO Displays the RATE GRAPH with the y axis rescaled so that the trace fills the screen GRAPH HIGH GRAPH LOW Allow the user to set the upper and lower limits for the RATE GRAPH y axis Relative Absorbance RESCALE AUTO RANGE AUTO Displays the RATE GRAPH with the y axis rescaled so that the trace fills the screen RANGE Allows the user to set the upper y axis value ABS DISPLAY This option enables the RATE GRAPH to be redisplayed with Absolute Absorbance or Relative Absorbance values 49 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual SMOOTHING This option offers a pop up menu to choose one of three smoothing algorithms Choices are NONE LOW MEDIUM and HIGH A moving point average is performed on the data and in each case data points will be lost from either end of the data SMOOTHING No of POINTS USED POINTS LOST AT EACH END NONE 0 0 LOW 9 4 MEDIUM 17 8 HIGH 37 18 ORIGINAL This removes any manipulation and displays the rate graph as originally specified by the rate method ANOTHER CELL than one rate has been run in parallel using the Cell Programmer then
39. UV Visible System Validator Issue 5 June 2003 NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN TEST PROGRESS SHEET 1 OF 2 System Customer generated number for the complete system 5 entered on the spine of the Validator Log i est Ref 1 Pass Pass Fai Fail Fail Date Result Pas Pav Pass Fail Fail Tester Initials Tester Initials Tester Initials PP3 Date Result Date Result Tester Signature Teste nitia DATE checkc Print Name Witness Signature Sign only if this page is completed Print Name Page 11 of 14 UV Visible System Validator Issue 5 June 2003 NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN This page is intentionally left blank Page 12 of 14 UV Visible System Validator Issue 5 June 2003 NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN TEST PROGRESS SHEET 2 OF 2 Test Ref Run Number 1 Tester 2 Tester 3 Tester Initials Initials Initials PP Date Resul Pass Pass Pass Fail Fail Fail PP13 Date Result PP8 L x F Pa Pass Pass Fai Fail Fail PP9 Date Result Pas Pass Pass Fail Fail Fail PP10 Date UN Result PP11 Date Res PP12 Dat Resu Tester Signature Testei iitial cock 45 f Print Name Witness Signature Sign only if this page is completed DATE Print Name Page 13 o
40. When ON results methods and sample IDs when selected are exported automatically after each measurement to the central LIMS computer via the RS232 port THIS INTERFACE MUST BE CONNECTED BEFORE LIMS SUPPORT IS ACTIVATED USE SAMPLE IDS Choose between OFF SEEDED and PROMPT USER OFF The system does not attach an identity to the sample SEEDED Enables the system to be set up to attach an identity to each sample automatically This appears on the screen and the print out It is also exported to the LIMS when enabled with the results of the run and method used Selection of SEEDED causes two additional items to appear in the Environment Menu SAMPLE ID Enter the name of the sample via the Text Entry Page Use the Arrow keys to move the cursor to the required character and press ENTER Once all the required characters je to 11 have been entered press ACCEPT If you make a mistake olojol will act as a backspace or CLEAR will clear the whole entry SAMPLE ID SEED Sets the number to be used for the first sample via the numeric keypad The Sample ID is incremented automatically before each run Set the seed to zero for the results of the first run to be numbered 1 PROMPT USER Before each run the Text Entry Page appears and the user is prompted to enter an identity for the sample NOTE a When the Cell Programmer is used in AUTO mode the Sample ID is incremented automatically without stopping for ID confirmati
41. ailable directly from the LAMPS key on the SETUP page If User Log on is not in use the HOME page will also have a LAMPS key This page shows the status of the Tungsten Halogen Deuterium and Mercury lamps whether ON OFF or FAILED and their appropriate energy levels It also allows the lamp hours to be reset and the Deuterium and Mercury lamps to be switched on or off THE MERCURY LAMP SHOULD BE SWITCHED OFF WHEN NOT IN USE HOURS parameter states the number of hours that the lamp has been in use The Tungsten Halogen lamp should be replaced after 2000 hours The Deuterium lamp should be replaced after 1000 hours Whenever a lamp is changed then the hours parameter should be reset to zero Q TheLAMP ENERGIES are measured as follows TUNGSTEN HALOGEN LAMP Measured at 546 nm DEUTERIUM LAMP Measured at 250 nm MERCURY LAMP Measured at zero order 18 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual LAMPS TUNGSTEN ON HOURS 987 ENERGY 99 D2 STRIKING HOURS 234 ENERGY 96 Hg OFF HOURS 2 ENERGY 0 RESET HOURS Displays a menu to choose whether the tungsten deuterium or mercury lamp is to be reset When OII selected resets hours and measures energy at the appropriate wavelength ALLOW THE LAMP AT LEAST 10 MINUTES TO WARM UP BEFORE RESETTING ITS HOURS ENERGY Displays a menu to choose whether the energy of the tungsten deuterium or mercury lamp is t
42. and decimal point Function Keys Access and perform system functions Operation depends on Screen in use and is indicated by labels at bottom of screen Clear Clears entry leaving field or parameter ready for new entry clears pop up and clears error messages Enter Enters changes to field or parameter Run Starts instrument measurement according to current method Home Returns to Home page Zero Base Performs a zero or baseline as appropriate to application Indicator LEDs Lower green ON upper red STANDBY REMOVE THE SAMPLE AND ENSURE THAT BOTH SAMPLE AND REFERENCE BEAMS ARE CLEAR OR CONTAIN THE BASELINE SAMPLES RELEVANT TO THE ANALYSIS BEFORE ZEROING THE INSTRUMENT OR PERFORMING A BASELINE SCAN 4 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual 5 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Software The Local Control Software is organised in a tree structure with all functions accessed initially from the HOME Page Movement between the software pages is controlled by function keys or by highlighting with cursor keys and pressing ENTER Home will always go to the HOME page The SCAN FIXED QUANT RATE and MCA applications are entirely separate Only one application can be operational at any one time Loading another application will overwrite any current data Local and Computer Control From switch on the instrument will automatically be under the HO
43. are loaded from Library or Disk The Wavelength s Bandwidth Integration Delay time Lamp change and Operator Name are also loaded with the standards and cannot be edited Any attempt to do so causes the prompt Change method by loading MCA standards to appear and no action is taken Changing the MEASURE STANDARDS parameter will cause all previous data to be lost STANDARDS Switches to the STANDARDS ENTRY screen Identifications and concentrations of up to 20 standards may be entered UNITS Switches to the TEXT ENTRY screen Enterthe units for the concentration WAVELENGTH S Switches to the WAVELENGTH ENTRY screen WAVELENGTH The available wavelength range is 190 0nm to 900 0nm If the wavelength requires the Deuterium lamp then this will be switched on The current data will be lost if the wavelength is changed BANDWIDTH Select from 4 2 1 5 1 0 5 and 0 2nm 53 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual INTEGRATION Enter integration time in seconds This sets the integration time for which the result is measured The minimum is 1s and the maximum is 9999s The current data will be lost if the integration time is changed DELAY TIME Set a time in the range 00 00 to 99 59 using to separate minutes and seconds The number of seconds must always be entered explicitly This sets the time between pressing RUN and the start of the measurement The range is from 0 to 99 minutes
44. asic Macros Wro Magna IR Microcast uView Molecular Micro Mapping Momentum MonitIR Mosaic Nexus NicALAS Nicolet Nicolet Compact Four Nicolet Pathfinder Nic Plan NXR NYSTAR OMNI Sampler OMNI Transmission OMNIC Passport Performer Phoenix PLS Quant PLUS ProfilIR Progressive Sampling PROSTAR Prot g QC Complete QCIRCLE Quadrascan QuantPad Quick IR ReactIR Recorder Redundant Aperturing Reflachromat Reflex REGA RESULT RSVP SabIR SAGA Sample Plan Satellite SeaPort Si Carb SID Simul View Slide ON ATR Smart Accessory Smart ARK Smart Collector Smart Purge Snap In Solid Substrate SpecID SpecID Plus Spectra Bench Spectra Fax Spectra Scope SpectroPro SpeculATR SPECTRONIC SPIRIT SST Swap Top SX Sync TACT Talon Targeting Thermo Nicolet Corporation Thunderdome Total Coverage TQ Analyst Triton TruView TVF UpDRIFT Validate Validator ValPro Val Q Val Q DS Vectra Vectra Plus ViewThru Viking Viking II VISION ife VISIONIite VISIONpro VISIONsecurity WinFIRST WinFIRST Lite and WinSpec are trademarks of Thermo Electron Scientific Instruments Corporation a subsidiary of Thermo Electron Corporation Nicolet Evolution 500 Local Control Software Manual CONTENTS ata kaa m 3 Ai SS ua a data un S Ga Qan Si 3 User interfaca mrsa Ed 4 Sm CE 6 SETUP inue RUD enini id
45. ator 5 Log Book Where appropriate references will be made to the required manual 1 1 Identification Nicolet Evolution 500 Series Local Control Software 1 2 Manufacturer Thermo Electron Corporation Mercers Row Cambridge CB5 8HY UK 1 3 General Description Nicolet Evolution 500 spectrophotometers have been designed for analytical applications in which fast accurate and reproducible spectrophotometric measurements are required The Local Control software provides control of the spectrophotometer via the use of the integral keypad and liquid crystal display 1 4 Compliance Features Features designed to assist the laboratory in recording and providing documentation for compliance include the following User log on and access control e All data is tagged with a unique system identifier which includes instrument type and serial number software version number etc e All data is date amp time stamped e All analytical calibrations are stored with the data file e n built calculations optional using uvcalc software 2 Product History Since its initial release the Local Control software has been updated at specified intervals to provide additional functionality both in terms of software capability and accessory control and to implement corrective actions Each successive release is identified by a unique version number displayed on the liquid crystal screen Page 3 of 14 UV Visible System Validator Issue 5
46. based on these measurements In UV Visible spectrophotometry and many other mature techniques the science associated with the measurement of the parameter is well developed There are fully validated test kits available from the leading chemical suppliers in the key areas of bio chemical and environmental water chemistry In addition most laboratories also have their own fully developed internal procedures With defined procedures many standard methods will document the final calculation in the form of an algebraic formula Uvcalc allows these formulae to be entered in to the software method together with the control limits providing automatic calculation of results from measurements using user defined equations and indication of conformity to the supplied control limits The measurement is obtained from the spectrophotometer in the form of a reading at a specific wavelength in Scan methods and individual results in Fixed and Quant methods This manual should be read in conjunction with the main instrument operating manual and also the uvcalc aqua or uvcalc bio manual where these have also been purchased Specification Up to 4 different equations may be applied to each measurement The formula editor supports and bracketing Allowed operands include Measurements Constants entered via the numeric keypad fixed amp variable factors input by the user at run time and uvcalc results from preceding equations Each
47. ce all results have been collected save the data QUANT METHOD Page QUANT I ID I WAVELENGTH 550 0 nm BANDWIDTH 2 0 nm INTEGRATION 18 STANDARDS 3 REPLICATES 1 UNITS CURVE FIT LINEAR LAMP CHANGE 325 nm OPERATOR UVCALC 0 I MEASURE STDS YES CELL PROG AUTO REF MODE _ _ L OFF _____ ID Enter a description using the TEXT ENTRY screen ThelD identifies the method and will be saved with the method and any results produced by the method WAVELENGTH The available wavelength range is 190 0nm to 900 0nm If the wavelength requires the Deuterium lamp then this will be switched on The current data will be lost if the wavelength is changed BANDWIDTH Select from 4 2 1 5 1 0 5 and 0 2nm INTEGRATION Enter integration time in seconds This sets the integration time for which the result is measured The current data will be lost if the integration time is changed STANDARDS Brings up the Standards Entry Page Use the up and down arrow keys to move through the list of standards When the standard to be entered or edited is highlighted press ENTER to display the Edit pop up Use the numeric keys to enter the concentration of the standard and press ENTER when finished The instrument returns to the Standards Entry page with the highlight on the next standard on the list Up to 20 standards can be specified Changing the standards will cause any
48. e Cell Programmer a The 8 Cell Programmer can be used in conjunction with the RATE software to measure between 2 and 8 cells in parallel For the Cell Programmer to be used it must be ON with MODE set to AUTO The RATE METHOD setup and MANIPULATE functions are exactly the same except that the MEASURE TIME now sets the time between each cycle i e the length of time between measurements on the first cell The number of measurements taken on each cell is set by the CELL CYCLES parameter on the cell programmer page The total time over which the measurements are made is the result of the time between measurements MEASURE TIME and the number of measurements CELL CYCLES For example an analysis using 4 cells with MEASURE TIME set to 15 seconds and CELL CYCLES set to 20 would give a total measurement time of 5 minutes To set up a parallel rate method do the following Q Set up the method as normal on the RATE PARAMETERS page Note that MEASURE TIME specifies the time between each measurement on the first cell Q Go to the Cell Programmer page Press HOME then ACCESSORIES and select CELL PROG Set up the cell programmer method as required Return to the RATE PARAMETERS page press ZERO BASE to zero the instrument if required then press RUN The analysis will proceed with the rate graph for the first cell being drawn on screen To view the result of any other cell press MANIPULATE on the RATE GRAPH page and select ANOTH
49. e Manual This page is intentionally left blank 31 Issue 1 August 2003 FIXED Page Nicolet Evolution 500 Local Control Software Manual FIXED 32 19 01 98 0 281A 19 01 98 0 281 11 35 FIXED 550 0nm 5 5500 FIXED ABS MODE ABS ID f1X123 11x123 From FIXED k SELECT 1 SINGLE SELECT SINGLE WAVELENGTH S 550 0nm WAVELENGTH S 550 0nm page c hoose BANDWIDTH X SELECT BANDWIDTH 2 0nm INTEGRATION INTEGRATION 18 DELAY TIME SINGLE DELAY TIME 0 00 sel ect ty pe LAMP CHANGE MULTI LAMP CHANGE 325nm OPERATOR SERIAL X OPERATOR Name lt lt Ss CELL PROG UVCALC 0 REF MODE CELL PROG AUTO REF MODE OFF PRINT SAVE VIEW PRINT SAVE VIEW METHOD METHOD RESULTS METHOD METHOD RESULTS 19 01 98 0 281 11 35 5 FIXED 550 0nm MODE ABS ID f1X123 SELECT MULTI X WAVELENGTH S 550 0nm BANDWIDTH INTEGRATION MODE DELAY TIME LAMP CHANGE Anc OPERATOR eT CELL PROG REF MODE PRINT SAVE EW METHOD METHOD ABS or 19 01 98 78 96 11 35 FIXED 550 0nm MODE ID f1X123 SELECT MULTI WAVELENGTH S 550 0nm BANDWIDTH 2 0nm INTEGRATION 1 DELAY TIME 0 00 LAMP CHANGE 325nm
50. ed wavelengths select PEAK TABLE TRACK then press VIEW GRAPH You do not have to have a spectrum present on the graph to enter the selected wavelengths Press MANIPULATE and select TRACK For each wavelength move the cursor to the desired position press ENTER Once all the wavelengths have been entered go back to the SCAN METHOD page and save the method RATIO This function allows the ratio 24 4 to be automatically calculated at the end of the scan To enter the desired wavelengths select PEAK TABLE and press ENTER then select RATIO A pop up box appears in which to enter the first wavelength Enter the desired wavelength and press ENTER Repeat for the second wavelength Once all the method parameters have been set save the method CORR RATIO This function allows the ratio of two wavelengths to be calculated relative to a third wavelength 4 43 5 2 5 automatically at the end of a scan To enter the desired wavelengths select PEAK TABLE and press ENTER then select CORR RATIO A pop up box appears in which to enter the first wavelength Enter the desired wavelength and press ENTER Repeat for the second and correction wavelengths Once all the method parameters have been set save the method PEAK HEIGHT This function allows the height of a peak to be calculated relative to a local baseline rather than y 0 To enter the desired 23 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual wavelengths select PEAK T
51. eters of Wavelength Accuracy Absorbance Accuracy Stray Light and Drift Only a pass in all of these parameters constitutes a pass to this test These procedures are best performed using a Thermo Electron Calibration Verification accessory a Calibration Validation Unit CVU Other suitable Certified Reference Materials CRMs may be used for checking the fundamental specification parameters or any of the tests specified in UVM 001 Located in the Validator 5 Log Book can be used as an alternative 4 6 Glossary Installation Qualification IQ Installation Qualification involves the checking of equipment and control system s against the supplier standards of operating environment physical connection safety parameters and functional parameters prior to the initial utilization of the system In other words confirming that the system is properly installed Operational Qualification OQ Operational Qualification is the process of demonstrating that the equipment will perform consistently as specified over all intended ranges CVU Calibration Validation Unit CVU NPL Nicolet Evolution 500 CVU NIST Nicolet Evolution 500 Certified Reference Material CRM certified traceable to International Primary Reference Materials by an ISO IEC accredited process 4 7 Approved Report Formats for System Acceptance Tests The following pages of the plan supply the plan Test Progress Sheet s for the documented tests Page 10 of 14
52. f 14 UV Visible System Validator Issue 5 June 2003 NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN This page is intentionally left blank Page 14 of 14 UV Visible System Validator Issue 5 June 2003
53. formula may have up to 20 characters Each formula will support up to 9 different of each of the measurements constants variable factors and results Equations will be saved with the current application method and uvcalc results saved with the current results Equations results units and pass fail statuses are included on the hardcopy output 58 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Operation General Comments On each of the scan fixed and quant pages a new line headed uvcalc will appear when the uvcalc software is installed With no equations programmed the field next to uvcalc will be 0 Pressing enter when the uvcalc line is highlighted will present a list of 4 uvcalc equations empty when first installed Highlighting and entering one of these will present the equations parameters page in the following format FORMULA TITLE UNITS TEST RESULT NO UPPER LIMIT 0 000 LOWER LIMIT 0 000 There will also be lines specific to the particular application scan fixed or rate see separate headings FORMULA To enter a formula select FORMULA and press ENTER A keyboard will appear with the following symbols M F R SPACE M Measurement On selecting this a pop up appears providing the choice between Once only and repeated measurements Replaced by in SCAN F Variable factor to be input by the user at run time R uvcalc result from preceding equatio
54. h SAVE METHOD Switches to the SAVE page To view further pages of results use the Up and Down arrow keys Pressing RUN takes another sample measurement and displays the result Results are numbered sequentially and any batch can be of up to 600 samples QUANT RESULTS ABS 1 0 201 2 0 201 CONC mg l 10 122 10 122 CLEAR RESULTS Deletes all results from the results table QUANT PAGE Switches back to the Quant page SAVE DATA Brings up the SAVE page PRINT LIST Gives a printout of the results LIMS EXPORT Sends the results via the RS232 port 43 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual 19 01 98 11 35 RATE ID WAVELENGTH 550 0nm BANDWIDTH 2 0nm MEASURE TIME 00 30 DELAY TIME 00 00 SLOPE POSITIVE RANGE 0 5 FACTOR 1 000 UNITS LAMP CHANGE 325nm OPERATOR Name CELL PROG AUTO PRINT SAVE VIEW VIEW METHOD METHOD GRAPH RESULTS Press Run 19 01 98 RATE GRAPH 0 281A 11 35 ID TEST 1 550 0nm 0 400 ABS MANIPULATE TRACK 0 200 SECTION RESCALE SMOOTHING ABS DISPLAY ORIGINAL ANOTHER CELL 0 000 00 00 00 15 00 30 PRINT SAVE RATE VIEW ULATE DATA PAGE RESULTS 19 01 98 TRACK 0 281A 11 35 ID TEST 1 550 0nm gt lt gt 0 400 4 i 5 i
55. he CELL PROG screen if fitted REF MODE Toggles the status of the Cell Programmer reference mode if fitted 34 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual FIXED Method Page Function Keys FIXED VIEW RESULTS Switches to the FIXED RESULTS page SAVE METHOD Brings up the SAVE page from where the method can be saved to Disk PRINT METHOD Prints the current method parameters on the printer Pressing RUN starts a fixed measurement using the current method and switches to the FIXED RESULTS page Q Pressing ZERO starts a zero using the current method Any changes to the WAVELENGTH BANDWIDTH INTEGRATION or LAMP CHANGE parameters will invalidate the current results If AUTOPRINTING is selected see SETUP for details a change to the MODE parameter will invalidate the current results FIXED RESULTS Page layout of the page depends on the Mode and Select in use SINGLE Up to 2 columns of results are displayed per page Results accumulate on the same page until it is full MULTI A Two columns of results are displayed per page Results of each sample always start on a new page SERIAL One column of results is displayed per page Results accumulate on the same page until it is full move up or down pages of results use the Up Down arrow keys Results are numbered sequentially from 1 to 600 35 Issue 1 August 20
56. he markers and the TRACK TABLE PRINT GRAPH Provides a hardcopy of the results showing the markers and x and y axis values SCAN GRAPH Returns to the SCAN GRAPH page 26 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual RESCALE RESCALE AUTO GRAPH HIGH GRAPH LOW GRAPH START GRAPH STOP PROCEED Q This option gives pop up menus for changing the graph x and y axis scales Move the cursor to one of the options and press ENTER to select an operation AUTO Displays the SCAN GRAPH with the x and y axes rescaled so that the Spectrum fills the screen GRAPH HIGH Pops up a window for entry of the GRAPH HIGH limit GRAPH LOW Pops up a window for entry of the GRAPH LOW limit GRAPH START Pops up a window for entry of the required start wavelength GRAPH STOP Pops up a window for entry of the required stop wavelength PROCEED Used after GRAPH HIGH GRAPH LOW GRAPH START or GRAPH STOP to return to the SCAN GRAPH page with the graph rescaled using the new parameters COMPARE When selected Compare goes to the LIBRARY page and displays a list of scan data files Highlight the desired file using the Up Down Arrow keys and press LOAD The reference spectrum is displayed as a dotted trace Q Once loaded the reference spectrum will remain on the screen and will be printed with all subsequent scans until removed To remove the reference spectrum from the display select MANIPULATE ORIGINAL or load a
57. ht up but no picture appear check the LCD contrast LCD display contrast can be controlled using a contrast wheel under the left side of the instrument PP2 System Initialization The spectrophotometer should now begin to initialize with pass mark appearing alongside each test as it is completed The Initialization page will automatically then Switch to the Home page Appearance of the page detailed above constitutes a pass to this test PP3 System Functionality Setup 1 Ensure the Local Control Screen is on the Home page if not press the Home key Page 6 of 14 UV Visible System Validator Issue 5 June 2003 NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN o NO nF 9 Press SETUP use the up down arrow keys to select Clock press Enter Select Hours press Enter Change the number to indicate the correct hours Press Enter Select Minutes press Enter Change the number to indicate the correct minutes Press Enter Select Day press Enter Change the number to indicate the correct day Press Enter Select Month press Enter Change the number to indicate the correct month Press Enter 10 Select Year press Enter 11 Change the number to indicate the correct year Press Enter Accept 12 Press Home Problem free operation of these processes and the display of the correct date and time constitute a pass to this test 4 5 4 PP4 System Functionality Scan 1 Ensure that the Application
58. is set to Scan if not press Home Scan Enter Set the scan type to standard the scan speed to 600 nm min the data interval to 1 nm the start wavelength to 435 nm and the stop wavelength to 475 nm Other wavelengths may be selected if a filter other than a holmium filter is used Place a holmium filter or other suitable filter in the sample position Press the Run button The appearance of a new spectrum on the display constitutes a pass to this test 4 5 5 PP5 System Functionality Data Store and Library 1 Ensure that the Application is set to Scan if not press Home Scan Enter Save the data produced from the PP4 System Functionality procedure Press Save Data enter the file name PP5 or another suitable file name Press Accept Save Check that the Data has been stored Home Library Enter Re enter the Scan application Press Home Scan Enter Scan new sample using the same conditions as PP4 This should be a different sample to that used for PP4 Recall the data produced in PP4 Press Home Library Enter PP5 or the file name used Enter Load Enter The saved spectrum should be visible on the display The successful completion of these tasks constitutes a pass to this test Page 7 of 14 UV Visible System Validator Issue 5 June 2003 NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN 4 5 6 4 5 7 4 5 8 4 5 9 4 5 10 PP6 Data Transfer to
59. ise a combination of up to 9 different one off measurements measured at the start of the run only and one measurement which will be re measured with each press of RUN Quant Up to 9 different measurements may be specified These measurements may be either a standard 51 56 measured as part of the normal calibration process or a sample X One off factors will be cleared if you select CLEAR RESULTS from the results page or if you return to the main menu UVCalc how to How to set up a Fixed calculation To set up the application M1 50 0 On the FIXED page go to the uvcalc line and press ENTER Go to EQUATION 1 and press ENTER Go to FORMULA and press ENTER Highlight M and press ENTER A pop up will appear giving the choice of ONCE ONLY CONSTANT MEASURE EACH RUN Highlight MEASURE EACH RUN and press ENTER Highlight and press ENTER Key in 50 60 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Press ACCEPT This will return you to the main uvcalc page Make appropriate entries for the TITLE and UNITS line Press ACCEPT twice This will return you to the FIXED page Insert sample and press RUN The results page will display the actual absorbance value and also the result of the calculation How to set up a Scan application To set up the application 24 42 F On the SCAN page go to the uvcalc line and press ENTER For this example ensure that START 400 nm and STOP 600 nm Go t
60. ists the standards as defined in the QUANT METHOD Before the system can be calibrated each standard must have a concentration entered To enter Standard concentrations use the up and down arrow keys to move through the list of standards When the standard to be entered or edited is highlighted press ENTER to display the Edit pop up Use the numeric keys to enter the concentration of the standard and press ENTER when finished The instrument returns to the Standards Entry Page with the highlight on the next standard on the list Up to 20 standards can be specified When all the standards have been entered press the ACCEPT function key to return to the QUANT Page with the new list of standards or CANCEL to return leaving the old list unchanged If a calibration has been done then the correlation coefficient and the equation are displayed If a calibration has not been done pressing RUN causes the warning prompt CANNOT RUN WITHOUT CALIBRATION to appear otherwise it takes a sample measurement and switches to the Quant Results screen Pressing ZERO BASE starts a zero using the current method 41 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual STANDARDS VIEW CALIB Switches to the QUANT GRAPH page if a valid calibration exists VIEW RESULTS Switches to the QUANT RESULTS page if there are any results in the table QUANT PAGE Switches back to the Quant page EDIT STD Available after calib
61. ited to the implied warranties of merchantability and fitness for a particular purpose Copyright 2003 by Thermo Electron Corporation Madison WI 53711 world rights reserved No part of this publication may be stored in a retrieval system transmitted or reproduced in any way including but not limited to photocopy photograph magnetic or other record without the prior written permission of Thermo Electron Corporation For technical assistance please contact Technical Support Thermo Electron Corporation 5225 Verona Road Madison WI 53711 4495 U S A Telephone 1 800 642 6538 U S A or 1 608 273 5015 worldwide Fax 1 608 273 5045 worldwide E mail techsupport analyze 9 thermo com ABBE 3L AIR Almega AMINCO Bowman Antaris Analyze Detect Measure Control ApPro AquaMate ARK Atlus ATMOS Aurora Avatar BACKCOMM Baseline BEAM BioMate Blocker Centaurus Chemigram CIRCLE Collector Contact Alert Contact Sampler Continuum Corner Cube CSA 4K DeterminatIR DSolve DX ECO Educator ENCORE Encompass Endurance E S P Ever Glo Evolution EZ Flow EZ Scope Far IR Solid Substrate FiberLink FiberPort FLEXSYS Flex Top Foundation Series Full Field Aperture Gemini Genesis II GENESYS G XT Helios ImageMax Impact Import Infinity Infinity Gold InspectIR Instruments of Discovery Integra Interlinking InterpretIR InterpretIR IR Plan IRus ISOBE Laser Quadrature Lock in Line Macros B
62. lculates the peak maximum relative to a local baseline SMOOTHING This option offers a pop up menu to choose one of three smoothing algorithms Choices are NONE LOW MEDIUM and HIGH A Savitsky Golay smooth is performed on the data and in each case data points will be lost from either end of the data SMOOTHING No of POINTS USED POINTS LOST AT EACH END NONE 0 0 LOW 9 4 MEDIUM 17 8 HIGH 33 16 ORIGINAL This removes any manipulation and displays the spectrum as originally collected and specified by the scan method It will also clear any Compare spectrum 28 Issue 1 August 2003 TRACK LIST MARK ABS A WAVELENGTH nm 1 0 472 447 0 2 2 002 463 0 CIO Nicolet Evolution 500 Local Control Software Manual The list shows the y axis values of the spectrum for the wavelengths marked during TRACK The measured values will be ABS INTENSITY or 1D 2D 30 4D depending on the current mode VIEW GRAPH Switches to the TRACK page LIMS EXPORT Sends the results via the RS232 port PRINT LIST Prints the list on the selected printer SCAN GRAPH Returns to the SCAN GRAPH page Ifa spectrum is saved then the track markers are also saved and will be displayed when the spectrum is reloaded PEAK TABLE Page Soe PEAK TABLE ABS A WAVELENGTH nm 1 0 472 447 0 2 2 002 463 0
63. ld always be done when one of the lamps is changed BASELINE This re measures the default baseline Ensure that both lamps are on and that the spectrophotometer is fully warmed up This process will take about one hour The default baseline should be re measured whenever one of the source lamps is changed or if the instrument is working at temperatures significantly different from 25 or if the wavelength calibration is altered INITIALISE WITH D2 This sets the instrument to initialise with or without the Deuterium lamp on If set to ON then the instrument will automatically strike the Deuterium lamp during initialisation 17 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual WHITE LIGHT Page The WHITE LIGHT feature is used to facilitate alignment of optical accessories in the sample compartment When the INITIALISE function key is pressed the instrument will align the grating so that the zero order diffraction passes through the sample compartment This provides a beam of white light which can be seen when a white card or similar is placed in the light path In double beam instruments the action of the chopper causes the light to alternate between the sample and reference beams When alignment is completed pressing the STOP function key returns the grating to its normal position and pressing the SETUP PAGE function key returns to the Setup Page LAMPS Page The lamp functions are av
64. lied in the Template Masters section of the Validator Log Book Testing will require the presence of two persons one to perform the test the other to act as a witness This requirement can be effectively met by having this validation process completed by a trained Thermo Electron authorized Customer Support Engineer witnessed by the Customer Contact your local Thermo Electron authorized Sales Support organization should you wish use this option 4 4 Testing Requirements To perform this System Acceptance Test the following must be available Nicolet Evolution 500 User Manual e A3 5inch floppy disk and a PC with Windows are required to verify data export e UVM 001 Analytical Procedures for a UV Visible spectrophotometer Calibration Validation Carousel CVC or other suitable CRMs for checking the fundamental specification parameters e g wavelength absorbance etc as detailed in UVM 001 e Suitable samples or filters for analysis 4 5 Test Procedures 4 5 1 4 5 2 4 5 3 PP1 Initial Power Up Apply power to the spectrophotometer Do not apply power to any connected PC After a pause of approximately 1 minute the LCD display should show the Initialization page The information at the top of the screen should display a valid date and time instrument type and serial number and will clearly vary from system to system Appearance of this page constitutes a pass to this test Note Should the display lig
65. m is composed of a spectrophotometer with integral keypad LCD display 1 44 Mbyte Disk Drive Local Control Software and output device Always remove disks from the disk drive when not in use Never power up the instrument with a disk in place as permanent damage may be caused to the disk The Local Control software controls all aspects of the systems operation The UVCalc software accessory is included as standard and provides automatic calculation of results from measurements using user defined equations UVCalc can be used in the SCAN FIXED and QUANT modes DEUTERIUM LAMP TUNGSTEN LAMP REFERENCE BEAM POWER ON OFF SAMPLE BEAM DISK DRIVE RS 232 FOR COMPUTER PRINTER SUPERSIPPER NS ee KEYPAD COMPARTMENT GONTRAST END PLATE CON LCD UNDER LEDs CELL PROGRAMMER 1 The only exception to this rule is when upgrading the instrument software Then automatic recognition of a software disk causes an automatic upgrade of the software 3 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual User Interface NUMERICAL KEYS LCD DISPLAY ARROW KEYS FUNCTION KEYS ON CLEAR ZERO BASE ENTER GREEN KEY KEY KEY STANDBY HOME RUN RED KEY KEY Key Operation Arrow Keys Highlight menu options or move track cursor or move Cell Programmer depending on page in use Change display contrast with lt gt from Home Page Numerical Keys Enter numbers minus
66. mp or recalibration using the Deuterium lamp Pressing ENTER toggles between them Q Press the SETUP PAGE function key to return to the SETUP page Q Press the CALIBRATE function key to begin calibration STOP becomes available during the calibration process It stops the calibration process and leaves the existing calibration unchanged Mercury Lamp Calibration This option will optimise the wavelength calibration of the instrument by measuring the position of the emission lines of the mercury lamp and fitting the calibration accordingly It can only be performed if the Mercury Lamp Accessory Part Number 9423 UV9 0023 is fitted and switched on It should only be attempted if for any reason the instrument no longer achieves its quoted wavelength accuracy specification Thecalibration will take at least 15 minutes Once the calibration is complete the Default Baseline must be run This operation will only need to be done if the existing calibration is corrupt the grating has been replaced or the monochromator drive has been replaced Q Proceed as follows Install the Mercury Lamp Accessory as detailed in the Mercury Lamp Accessory Installation and Maintenance Manual Ensure the Mercury Lamp is ON Ensure the instrument is fully warmed up and the beams are clear From WAVELENGTH CALIBRATION page select CALIBRATION USING Hg LAMP and press CALIBRATE Once calibration is complete switch both the Mercury Lamp and instrumen
67. new method MODE Select from ABS T 1D 2D 3D 4D ABS Selects Absorbance Selects transmittance 1D Selects first derivative This records the first derivative of the Absorbance spectrum 2D Selects second derivative This records the second derivative of the Absorbance spectrum 3D Selects third derivative This records the third derivative of the Absorbance spectrum 4D Selects fourth derivative This records the fourth derivative of the Absorbance spectrum 27 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual FUNCTION PEAKS VALLEYS PKS amp VALLEYS ZERO CROSS RATIO CORR RATIO PEAKS PK HEIGHT This option enables the spectrum to be automatically searched for peaks valleys or zero crossing points Move the cursor to one of the options and press ENTER to perform a search When the search is complete the spectrum is displayed with the peak positions marked For a peak to be found there must be more than 15 data points between that point and a previous peak For RATIO and CORR RATIO enter the wavelengths as prompted All results can be viewed by pressing OIOIOIOI8 view RESULTS PEAKS Marks the 10 highest peaks VALLEYS Marks the 10 lowest valleys PKS amp VALLEYS Marks the 5 highest peaks and 5 lowest valleys ZERO CROSS Marks the first 10 zero crossings RATIO Calculates the ratio 24 22 CORR RATIO Calculates the ratio A A3 A A3 PK HEIGHT Ca
68. nges x and y axis scales automatically or manually COMPARE Loads reference spectrum for comparison MODE Changes mode Select from T ABS 1D 2D 3D 4D PEAKS Finds spectral peaks Select from PEAKS VALLEYS PEAKS amp VALLEYS ZERO CROSS RATIO CORR RATIO PK HEIGHT SMOOTHING Applies LOW MEDIUM or HIGH modified improved Savitsky Golay smoothing to the spectrum ORIGINAL Resets the graph to display the data as originally collected TRACK the vertical cursor across the screen use the Left and Right Arrow keys cursor always moves to a data point regardless of the displayed scales Pressing ENTER places a marker at the current wavelength Up to 10 wavelengths can be selected TRACK page function keys Pressing CLEAR will delete markers in turn highest number first The x axis values are listed on the TRACK table page Further markers can be added to the spectrum at any time however selecting TRACK will cause any previous PEAK TABLE information to be lost VIEW TABLE Switches to the TRACK TABLE page FAST SLOW Toggles between two cursor speeds In FAST mode the cursor jumps 5 of the graph or to the next data point whichever is the greater In SLOW mode the cursor jumps to the next data point or the next display pixel whichever is the greater The function key label shows the next speed ie the opposite to the one selected CLEAR ALL Clears all t
69. ns The required formula can then be built up by highlighting the required symbol and pressing ENTER NB Navigation around the screen is achieved in all cases as per the normal operation of the software ie using the arrow keys to move around To clear the formula press To accept it press ACCEPT TITLE amp UNITS allow the user to enter a suitable description and units for the equation TEST RESULT toggles between amp OFF whilst the LIMIT lines set the allowable limits for the test 59 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Scan Up to 9 different measurements may be specified for each Scan uvcalc equation These are denoted by 1 9 The wavelengths at which these measurements are made can be specified in one of 2 ways They can be entered numerically before the scan They can be entered after the scan using a peak picking process This appears as an extra parameter on the equation parameter page USE TRACKING The selected wavelengths will then be fed into the method so that the subsequent final result will be calculated automatically One off factors are input by the user before the first scan If you subsequently move from the results graph pages back to the main menu the one off factors will be reset and must be entered again before the next run Fixed Up to 9 different measured results may be specified for each uvcalc equation These measurements will compr
70. o EQUATION 1 and press ENTER Go to FORMULA and press ENTER Highlight A and press ENTER An edit box will appear Key in 450 for the first wavelength and press ENTER Highlight and press ENTER Highlight and press ENTER The edit box will again appear Key in 500 and press ENTER Highlight and press ENTER Highlight and press ENTER A pop up will appear giving the choice of FIXED FACTOR VARIABLE FACTOR Highlight Variable Factor and press ENTER Enter a suitable ID and press ACCEPT Add suitable Title and Units Press ACCEPT twice 61 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Press RUN You will be asked to enter a factor The instrument will now scan and a results page will appear with the result of the calculation How to edit an existing method How to add parameters to the end of an equation e g to modify M1 50 0 by adding a weight correction so the equation becomes M1 50 0 F1 F2 Where F1 Nominal weight Fixed Factor F2 Actual weight Variable Factor On the FIXED page go to the uvcalc line and press ENTER Go to EQUATION 1 and press ENTER The equation prepared in section 5 1 should be displayed Go to FORMULA and press ENTER The cursor should be at the end of the existing formula Highlight and press ENTER Highlight and press ENTER Highlight F and press ENTER A pop up will appear giving the choice of FIXED FACTOR VARIAB
71. o be measured ALLOW THE LAMP AT LEAST 10 MINUTES TO WARM UP BEFORE MEASURING ITS ENERGY CLEAR BOTH SAMPLE AND REFERENCE BEAMS BEFORE MEASURING LAMP ENERGIES SWITCH Hg Switches the Mercury lamp ON or OFF depending on its current status THE MERCURY LAMP SHOULD BE TURNED OFF WHEN NOT IN USE SWITCH D2 Turns the Deuterium lamp ON or OFF depending on its current status 19 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual SCAN 20 SCAN page raso aid 550 018 SCAN INTELLISCAN To perform a scan set parameters ID TEST 1 FEA and press RUN START 400 0nm STOP 600 0nm BANDWIDTH DATA INTERVAL 1 0 PEAK TABLE orr SCAN GRAPH page GRAPH HIGH 2 000 GRAPH LOW 0 000 19 01 98 SCAN GRAPH 0 281A LAMP CHANGE 325nm 11 35 ID TEST 1 550 0nm OPERATOR Name UVCALC 0 2 000 _ CELL PROG AUTO REF MODE OFF ABS UVCALC PRINT SAVE VIEW VIEW RESULTS METHOD METHOD GRAPH RESULTS View Graph MANIPULATE page MI 19 01 98 SCAN GRAPH 0 281A 11 35 ID TEST 1 550 0nnj hr T AL fe lap Sa 0 000 2 000 Pr M 400 0 450 0 500 0 550 0 600 0 ABS MANIP PRINT SAVE
72. ode This will measure the intensity of the signal in the sample beam Note Set the EHT table to FLAT when scanning in Intensity mode 1D Selects first derivative This records the first derivative of the Absorbance spectrum 2D Selects second derivative This records the second derivative of the Absorbance spectrum 3D Selects third derivative This records the third derivative of the Absorbance spectrum 4D Selects fourth derivative This records the fourth derivative of the Absorbance spectrum The current spectrum will be lost if the Scan Mode is changed START Selects start wavelength 190 0nm to 896 0nm If the start wavelength requires the Deuterium lamp then this will be switched on The Start wavelength must be at least 4 nm less than Stop wavelength The current spectrum will be lost if the Start wavelength is changed STOP Selects stop wavelength 194 0nm to 900 0nm The Stop wavelength must be at least 4 nm greater than the Start wavelength The current spectrum will be lost if the Stop wavelength is changed BANDWIDTH Select from 4 2 1 5 1 0 5 and 0 2nm SPEED Selection depends on Scan Type selected Inlntelliscan mode select from ZIP SURVEY NORMAL QUANT HIGH RES VERY HIGH RES Standard mode select from 3800 2400 1200 600 240 120 30 10 or 1 nm per min DATA INTERVAL Sets the frequency of data points in the spectrum Selection depends on Scan Type In Intelliscan mode the data inter
73. on between samples b PROMPT USER is not compatible with the Sipper used in Sip and Run or AutoSampler modes AUTOSAVE RESULTS Toggles between ON and OFF When ON results are saved automatically after each run Selecting ON causes two additional items to appear in the Environment menu FILENAME Enter a filename of up to 5 characters via the Text Entry Page FILE NUMBER Enter a number between 0 and 999 via the numeric keypad The number is appended to the filename and incremented automatically after each run 13 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual AUTOPRINT RESULTS Toggles between ON and OFF When ON results are printed automatically after each run Before attempting to print at any point during operation of the instrument ensure that the printer is ready to print ie switched on on line and supplied with paper Failure to do so will result in an error condition Press Clear to clear the error message the system may take a little time to respond Ensure the printer is ready and retry USER LOG ON The default setting is OFF Changing the setting to ON is password protected When set to OFF any user has full access to all the functions of the instrument When set to ON users must identify themselves by user name and password at log on and then have access to whichever functions are enabled for them by the System Administrator Setting User Log on to ON is password protected
74. or 24 Pin Dot Matrix using ESC P language HP Laserjet Series HP Deskjet 500 Series and above Black amp White Compatible with plotters using HPGL language HP Deskjet 690C Colour HP Deskjet 400 Mono Printers not on the above list that claim Epson 9 pin 24 pin ESC P or HP PCL Programming Control Language Level 3 compatibility should work with the instrument but are not guaranteed to do so and are therefore not supported If in doubt contact your Thermo Electron approved Customer Support organization Before attempting to print using an external printer at any point during operation of the instrument ensure that the printer is ready to print Failure to do so will result in an error condition Press CLEAR to clear the error message Then rectify the problem with the printer and try again 11 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual ENVIRONMENT page This page is used to select the language used for the software the use of the beep date format and to enable disable Automatic Calibration Validation and LIMS Laboratory Information Management System Support to select the default filetype used when saving results and to select whether wavelength calibration validation is carried out using the mercury lamp or glass filters in the CVU with the tungsten lamp ENVIRONMENT LANGUAGE ENGLISH SOUND OFF DATE FORMAT dd MM yy AUTOMATIC CAL VAL OFF
75. pressing RUN and the start of the measurement The range is from 0 to 99 minutes and 59 seconds in steps of 1 second 45 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual SLOPE Select from POSITIVE or NEGATIVE Sets the graph to display positive or negative changes in Absorbance Choose POSITIVE if Absorbance increases with time Choose NEGATIVE if Absorbance decreases with time RANGE Set a number in the range 0 to This sets the graph y axis Enter a number slightly larger than the change Absorbance expected FACTOR Enter the factor for Activity as a number in the range 0 001 to 9999 999 UNITS Enters the units of Activity using the TEXT ENTRY page Enters the required description or units of Activity up to 11 alphanumeric characters LAMP CHANGE Select from 315 320 325 330 335 340 D2 W Hg Q Selects the wavelength at which the source is changed between the Tungsten and Deuterium lamps Selecting D2 W or Hg overrides any changeover and the selected lamp will be used regardless of the wavelength set THE MERCURY LAMP MUST BE SWITCHED OFF WHENEVER IT IS NOT IN USE OPERATOR Switches to the TEXT ENTRY screen operator name is automatically saved with the method any data produced by the method Changing the operator name will not cause the current data to be lost If User Log on is in operation the operator name cannot be changed CELL PROG Switches to the CELL
76. r discrete pairs of cursors can be placed on the graph Arrows are placed on the cursors and results are displayed on the RATE RESULTS page for those parts of the graph indicated by the arrows Selecting SECTION will remove the TRACK markers Q The minimum interval between TRACK cursors is one second TRACK VIEW RESULTS Switches to the RATE RESULTS page FAST SLOW Toggles between two cursor speeds In FAST mode the cursor jumps 5 of the graph or to the next data point whichever is the greater In SLOW mode the cursor jumps to the next data point or the next display pixel whichever is the greater The function key label shows the next speed ie the opposite to the one selected CLEAR ALL Clears all the markers RATE GRAPH Returns to the RATE GRAPH page 48 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual SECTION move the vertical cursor across the screen use the Left and Right Arrow keys The cursor always moves to a data point regardless of the displayed scales Pressing ENTER places a marker at the current time Todelete a marker place the cursor on the marker and press CLEAR Up to five markers can be placed on the graph Rate results will be reported between markers providing a maximum of four sets of results Sections The minimum Section size is one second Results are listed on the RATE RESULTS page Selecting TRACK will remove the SECTION markers RESCALE
77. ration Allows each standard to be used ignored or remeasured EDIT CURVE Available after calibration Allows curve fit to be changed QUANT CALIBRATION Q Press ZERO BASE to zero the instrument with the current method Tocalibrate the system press return to the QUANT page and press CALIBRATE The QUANT CALIBRATION graph will be displayed and the instrument will prompt for each standard and replicate in turn As the measurements of the standards proceed the datapoints are marked on the graph When all the standards have been measured the system calculates the equation rescales the graph then draws and displays the line of best fit on the graph A calibration can be stopped by pressing the STOP function key The calibration will be aborted and the software will return to the QUANT STANDARDS page Any values obtained will be lost Ifa calibration has not been done pressing RUN causes the warning prompt CANNOT RUN WITHOUT CALIBRATION to appear otherwise it takes a sample measurement and switches to the Quant Results screen 42 Issue 1 August 2003 QUANT RESULTS Page a a a 0 0000 50 000 COEFFICIENT 0 9999 Nicolet Evolution 500 Local Control Software Manual VIEW RESULTS Switches to the Quant Results page if there are results QUANT PAGE Switches back to the Quant page STANDARDS Switches to the Quant Standards page PRINT GRAPH Prints the Quant method and calibration grap
78. re STD1 will be displayed Put the first standard in the beam then press RUN Continue until all standards have been measured Results for each standard will be stored in the Calibration Results Table with results for each standard on a new page Use the up and down arrow keys to move between standards When the last calibration has been done put the first sample to be analysed in the beam and press RUN Analysing a Sample When a calibration has been performed or loaded with the method the MCA application is ready to use When RUN is pressed from the METHOD or RESULTS pages the instrument will measure the absorbance of the sample at each of the wavelengths specified in the method and compare with the absorbances of the standards at these wavelengths The concentrations of each component are calculated and displayed on the results page Results for each sample are displayed on a new page Use the up and down arrow keys to move through the pages of results Oll CLEAR RESULTS Clears the results table MCA PAGE Returns to the MCA METHOD page SAVE DATA Displays the SAVE page from which the results can be saved to Library or Disk PRINT LIST Prints the list on the selected printer 57 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual UVCalc Introduction Quantitative analytical procedures are built around two fundamental key principles measurement of the parameter and subsequent calculations
79. reads ACCEPT if edit is in progress SAVE saves file to disk and is not present during an edit CANCEL Abandons the naming operation No files will be changed and the software returns to the calling screen 7 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual SETUP 19 01 9 66 55 SERIAL No 100661 432 1nm HOME SCAN FIXED QUANT RATE MCA LIBRARY INSTRUMENT HOURS 12345 CAL ACCESS SETUP VAL ORIES REMOTE 19 01 98 66 55 T 11 35 432 1nm PRINTER ENVIRONMENT WAVELENGTH INITIALISE WHITE LIGHT 19 01 98 66 55 11 35 432 1nm PRINTERS PRINTER TYPE HP MONO PRINTER EPSON 9 PIN HP LASERJET HP MONO HP PAINTJET HP PLOTTER HP 690 HP 400 SETUP PAGE 19 01 98 66 55 11 35 432 1nm ENVIRONMENT LANGUAGE ENGLISH SOUND OFF DATE FORMAT dd MM yy AUTOMATIC CAL VAL OFF DEFAULT FILE TYPE NORMAL CVU WAVELENGTH TEST FILTER LIMS SUPPORT OFF USE SAMPLE IDS SEEDED SAMPLE ID SAMPLE ID SEED 5 AUTOSAVE RESULTS OFF AUTOPRINT RESULTS OFF USER LOG ON OFF HISTORY FILE ON SETUP UVCALC PAGE OFF 19 01 98 UNICAM UV310 v5 0 66 55 11 35 432 1nm WHITE LIGHT SETUP INITI PAGE ALISE
80. ressing RUN starts a measurement using the current method Q Pressing ZERO BASE zeros the instrument using the wavelength specified in the current method See page 4 MANIPULATE TRACK SECTION RESCALE SMOOTHING ABS DISPLAY ORIGINAL ANOTHER CELL 47 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual TRACK Sets the start and stop time for the rate calculations SECTION Sets sequential start and stop times to enable rates to be calculated on up to four sections of the rate curve RESCALE Changes y axis scale automatically or manually SMOOTHING Allows three levels of smoothing to be applied to the Rate Curve ABS DISPLAY Sets the display of absolute absorbance values or absorbances relative to the first measurement s of the run ORIGINAL Resets the graph to display the data as originally collected ANOTHER CELL Only present if the Cell Programmer has been used Enables the results of another cell from the same run to be displayed TRACK Tomove the vertical cursor across the screen use the Left and Right Arrow keys cursor always moves to a data point regardless of the displayed scales Pressing ENTER places a marker at the current time To delete a marker place the cursor on the marker and press CLEAR x axis values are used to recalculate the rate of change of Absorbance between the new start and stop times Results are listed on the RATE RESULTS page Up to fou
81. stitute a pass to this test PP9 System Functionality Rate 1 Ensure the Application is set to rate if not press Home Rate Enter 2 Press the run button and follow any prompts A graphical display of absorbance against time on the Rate Graph screen constitutes a pass to this test PP10 System Functionality Multicomponent Analysis This test requires a holmium filter and a didymium filter If only one wavelength filter is available a 1 A filter may be used in place of the second wavelength filter Page 8 of 14 UV Visible System Validator Issue 5 June 2003 NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN Ensure the Application is set to Multicomponent Analysis if not press Home MCA Enter Setup an MCA method as follows Ensure that Measure Standards is set to Yes if not highlight and press Enter Highlight Standards and press Enter Set up the Standards Table as follows Standard 1 ID HO Concentration 2 Standard 2 ID Concentration 6 Press Accept Highlight Wavelengths and press Enter Enter the following wavelengths 446 454 530 and 586 nm Press Accept to return to the MCA Method page Press the Calibrate function key When prompted for the first standard put the holmium filter in the sample beam and press Run When prompted for the second standard put the didymium filter in the sample beam and press Run This completes the calibration Now run a sample Put the Holmi
82. strument History PRINT HISTORY The Instrument History is printed out Make sure the printer is connected and ready before selecting PRINT HISTORY The History File contains a maximum of 400 entries When the number of entries reaches 390 a warning message is displayed and it is necessary for the Administrator or a user with the History File privilege save to disk and or print the existing history file then clear the history file to make room for more entries ENVIRONMENT HISTORY FILE Appears when History File is enabled CHANGE USERS Available only to Administrator when User Log on enabled SETUP PAGE Returns to the SETUP page 15 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual WAVELENGTH CALIBRATION Page From this page the Wavelength calibration of the instrument can be adjusted CALIBRATION Select between USING HG LAMP and USING D2 LAMP WARNING DO NOT ATTEMPT TO RECALIBRATE THE INSTRUMENT UNLESS YOU ARE ABSOLUTELY SURE YOU NEED TO DO SO IF IN ANY DOUBT CONTACT THERMO ELECTRON APPROVED CUSTOMER SUPPORT To find out whether recalibration is needed it will be necessary to check the Wavelength Accuracy This is most conveniently done with the optional CVU and Self Test Accessories Performing a Recalibration ENSURE BOTH SAMPLE AND REFERENCE BEAMS ARE CLEAR BEFORE ATTEMPTING ANY WAVELENGTH CALIBRATION There two options calibration with the optional Mercury la
83. t OFF Switch the instrument ON and allow to warm up Run the Default Baseline 16 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Deuterium Lamp Calibration This option will optimise the wavelength calibration of the instrument by measuring the Deuterium lamp emission line at 656 1nm and fitting the calibration accordingly It should only be attempted if for any reason the instrument no longer achieves its quoted wavelength accuracy specification calibration will take at least 10 minutes Proceed as follows Ensure Deuterium Lamp is ON Ensure instrument is fully warmed up and the beams are clear From WAVELENGTH CALIBRATION page press CALIBRATE Once calibration is complete switch the instrument OFF Switch instrument and Deuterium lamp ON and allow to warm up Run Default Baseline as detailed below OPTICAL INITIALISATION Page This page is used to reset the instrument and define its initialisation and default baseline These procedures ensure the optimum performance of the spectrophotometer INITIALISATION TYPE When selected this displays a pop up menu to choose between initialisation of optics or baseline OPTICS During the normal initialisation procedure the spectrophotometer optimises the alignment of the lamp change mirror over a predefined range to give the maximum energy throughput this operation defines the range over which the optimisation takes place This shou
84. t the list and return to the MCA Methods page Wavelengths can be selected either from a scan of the mixture to be analysed or by performing a scan on each standard in turn and selecting suitable wavelengths Wavelengths already entered in the table are shown on the Scan Graph At least one wavelength must be entered for each standard og ACCEPT Transfers to the MCA METHODS page with all marked wavelengths added to the wavelength list FAST SLOW Toggles between two cursor speeds In FAST mode the cursor jumps 5 of the graph or to the next data point whichever is the greater In SLOW mode the cursor jumps to the next data point or the next display pixel whichever is the greater CLEAR ALL Clears all marks RESCALE Rescales the x and y axes so that the spectrum fills the screen 56 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual Calibration When the Standard Identifications and concentrations have been entered via the STANDARDS ENTRY page and the wavelengths have been entered via the WAVELENGTH ENTRY page the MCA application is ready for calibration Clear the beam s and press ZERO BASE to perform a baseline scan Press the CALIBRATE function key from the MCA METHODS page If any results are present you Will be given the option to proceed or cancel with PROCEED highlighted All previous data will be lost if PROCEED is selected Press ENTER to proceed The message Press RUN to measu
85. too high then the detector will saturate Toselect the table press ENTER NORMAL This is the table for normal use HIGH ABS Use this table for highly attenuating accessories FLAT Use this table when scanning in intensity mode Normally the EHT table compensates for variations in the source intensity Consequently use of the NORMAL or HIGH ABS options will result in major loss of detail in the intensity spectrum To recalibrate any of the tables first select the table to be calibrated and then press CALIBRATE CAUTION DO NOT RECALIBRATE THE EHT TABLES UNLESS YOU ARE ABSOLUTELY SURE YOU NEED TO DO SO EHT CALIBRATION CALIBRATE Calibrates the selected EHT table SETUP PAGE Returns to the SETUP page 10 Issue 1 August 2003 PRINTERS Page Nicolet Evolution 500 Local Control Software Manual This page sets the system to work with the selected printer THE PRINTER MUST BE FITTED WITH A SERIAL INTERFACE PRINTERS DESIGNED TO WORK ONLY IN A WINDOWS ENVIRONMENT ARE NOT COMPATIBLE WITH LOCAL CONTROL SOFTWARE printer type is always highlighted The default printer is HP Mono To choose printer press ENTER to display the list of supported printers and select using cursor keys Press ENTER to confirm entry Printer option EPSON 9 PIN HP LASERJET HP MONO HP PLOTTER HP 690C HP 400 PRINTER EPSON 9 PIN HP LASERJET HP MONO HP PLOTTER HP 690C HP 400 Supported Printers Epson 9
86. try Supplier Guidance Validation of Automated Systems in Pharmaceutical Manufacture later formally adopted as the GAMP Guidelines 4 System Acceptance Test Specification 4 1 Introduction Author Dr Craig Fellows Version Number 5 Title Product Manager This document describes the tests required for the specific Installation Qualification IQ and Operational Qualification OQ of the Nicolet Evolution 500 Local Control Software and references appropriate sections of the user documentation 4 2 Scope This test specification details the Qualification procedures required for Initial Power Up System Initialization System Functionality Data Save and Transfer Spectrophotometer Compliance to Specification 4 3 Overview and Test Plan Section Section 4 5 describes in detail the tests required for this procedure Each test has its own unique identifier Results should be recorded on the Test Progress Sheet supplied with this plan If the result of the test is a pass the Pass should be circled If the result is a failure the Fail Page 5 of 14 UV Visible System Validator Issue 5 June 2003 NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN should be circled If a test is hardware or software specific and is not present the N A should be entered N A meaning Not Applicable In the event of a test failure and corrective action taken this should be reported on a copy of the Test Incident Sheet a master of which is supp
87. ue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual UVCalc error messages The following error messages may occur if you make a mistake in entering an equation or in setting the system up ONLY 1 FACTOR MAY BE ENTERED WITH SAMPLE You have attempted to create a formula with two or more factors for each sample THIS FORMULA HAS TOO MANY CONSTANTS You have created a formula with more than 9 numbers in it FORMULA CONTAINS AN INVALID NUMBER You have attempted to create a formula with an invalid input BRACES DO NOT MATCH IN FORMULA You have created a formula with too many brackets at one end ALL BINARY OPERATIONS REQUIRE TWO OPERANDS You have created a formula with an incomplete arithmetical operation e g 3 4 INVALID COMBINATION OF OPERANDS You have created a formula with missing operator s e g F1 M1 BRACE MISSING UNMATCHED CLOSE BRACE You have created a formula with a close brace bracket before or without an open brace THIS FORMULA CANNOT START WITH THIS TOKEN In QUANT mode you have created a formula with an invalid initial token i e an operator rather than an operand FORMULA CONTAINS OUT OF RANGE STANDARD A specified standard is no longer in the calibration ONLY ONE MULTIPLE MEASUREMENT IS ALLOWED In FIXED mode you have created a formula with an invalid initial token FORMULA CONTAINS INVALID RESULT TOKEN The result from an earlier calculation is no longer being produced UVCALC INVALI
88. um filter in the sample beam and press Run Completion of the measurements and calculation of concentration values constitute a pass to this test 4 5 11 PP11 1 2 System Functionality UVCalc Bio Load the method CELLGROW FXD from the UVCalc Bio floppy disk Home Library View Disk CELLGROW FXD Enter Load Enter Press Run New data on the Fixed Results Screen constitutes a pass to this test 4 5 12 PP12 System Functionality Access Control Ensure the Local Control Screen is on the Home page if not press the Home key Press Setup Environment User Log on Enter Enter ADMIN and press Accept Press Change Users Add a new user called TEST move the cursor to an empty field and press Enter enter the Name TEST and press Accept Add the password TEST for the new user TEST press Enter enter the password TEST and press Accept Press Accept Home Log Off Proceed Enter 8 9 Verify a choice of users is now displayed Select ADMIN press Enter type the password ADMIN press Accept 10 Press Setup Environment User Log on Enter The successful completion of these tasks constitutes a pass to this test Page 9 of 14 UV Visible System Validator Issue 5 June 2003 NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN 4 5 13 PP13 System Operational Qualification Spectrophotometer Compliance to Specification Check the fundamental instrument param
89. user will be prompted to log on by entering name and password At the close of a session the user logs off by pressing the LOG OFF function key on the Home Page and choosing 14 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual whether to PROCEED or STOP When PROCEED is chosen the system waits for the next user to enter their name USER LOG ON can be reset to OFF only by the Administrator After USER LOG ON has been set to OFF the list of users is cleared and the default User Name and Password are both reset to ADMIN HISTORY FILE Toggles between ON and OFF The History File contains the history of the instrument An entry is put into the file when there are changes to default baselines wavelength calibrations EHT calibrations sipper calibrations and CVU tests noting date time and user The file will also contain records of operations carried out by engineers during maintenance visits When HISTORY FILE is set to ON the HISTORY FILE function key becomes available on the Environment Page unless USER LOG ON is enabled and the user has been denied access by the Administrator Pressing this function key brings up the History File Pop up box SAVE HISTORY ON DISK The user is prompted for a HISTORY FILE file name and the instrument history is saved in CSV format which may be read by a suitable spreadsheet or SAVE HISTORY ON DISK text editor CLEAR HISTORY PRINT HISTORY CLEAR HISTORY Clears the In
90. val is fixed according to the intelliscan type selected Intelliscan Interval between points ZIP 4 nm SURVEY 2 nm NORMAL 1 nm QUANT 0 5 nm HIGH RES 0 2 nm VERY HIGH RES 0 1 nm 22 Issue 1 August 2003 Nicolet Evolution 500 Local Control Software Manual In Standard mode the choice of data interval depends on the scan speed selected Speeds Data Intervals 3800 4 2400 4 2 1200 4 2 1 600 4 2 1 0 5 240 4 2 1 0 5 0 2 0 1 120 4 2 1 0 5 0 2 0 1 30 4 2 1 0 5 0 2 0 1 10 4 2 1 0 5 0 2 0 1 1 4 2 1 0 5 0 2 0 1 The current spectrum will be lost if the scan speed is changed PEAK TABLE Select from OFF PEAKS VALLEYS PKS amp VALLEYS ZERO CROSS TRACK RATIO CORR RATIO and PEAK HEIGHT This selects the type of peak picking done automatically as part of the method Results are reported on the Peaks Page Peaks information is stored with any saved spectrum OFF Sets Peak Table to Off No peaks information is produced as part of the scan PEAKS Picks the highest peaks in a spectrum up to a maximum of 10 peaks VALLEYS Picks the lowest troughs in a spectrum up to a maximum of 10 troughs PKS amp VALLEYS Picks the 5 highest peaks and the 5 lowest valleys ZERO CROSS Picks all the points where the spectrum crosses zero up to a maximum of 10 crossing points TRACK This function allows the Absorbance or other mode values to be reported at up to 10 user selected wavelengths To enter the desir
91. vides control of the spectrophotometer via the use of keypad and liquid crystal display Under the five identified applications of Scan Fixed Quant Rate and MCA it provides the functionality of wavelength scanning single fixed wavelength measurement quantitation using a calibration graph constructed from standards of known concentration and absorbance vs time measurements Mass data storage is provided by an instrument memory library or by writing to Page 4 of 14 UV Visible System Validator Issue 5 June 2003 NICOLET EVOLUTION 500 LOCAL CONTROL IQ OQ PLAN a PC compatible disk In addition to this basic functionality there are control functions for spectrophotometer set up accessories performance qualification customized calculations etc 3 1 Thermo Electron Accessories Directly Supported e Calibration Validation Unit CVU SuperSipper e 8 Cell Changer 3 2 Product Documentation Nicolet Evolution 500 User Manual 3 3 Product Development amp Evaluation This product has been developed and evaluated according to the procedures placed on the Thermo Electron Corporation by the ISO 9001 registration 3 4 Qualification User Installation The processes detailed below form an essential part of any System Validation These documents and their structure are based on the requirements of the UK Pharmaceutical Industry Computer Systems Validation Forum PICSVF These guidelines were published in their document Pharmaceutical Indus

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