H201-LUDL-96A602 / Issued
Contents
1. Greiner T25 flask 2 35mm Petri dish 2 1x3in chamber slides www oko lab com be H201 LUDL 96A602 User Manual March 2015 2 Lab Tek 1in x2in chambered cover glass 2 Lab Tek II 1in x2in chambered cover glass 2 60mm Petri dish 4 35mm Petri dish 1 1x3in chamber slide and 2 35mm Petri dish 1 Lab Tek 1in x2in chambered cover glass and 2 35mm Petri dish 1 Lab Tek II 1in x2in chambered cover glass and 2 35mm Petri dish 1 Lab Tek 1in x2in chambered cover glass and 1 60mm Petri dish 1 Lab Tek II 1in x2in chambered cover glass and 1 60mm Petri dish 1 1x3in chamber slide and 1 60mm Petri dish 1 60mm Petri dish and 1 35mm Petri dish Magnetic lock to hold the standard MW plates in the chamber Magnetic lock to hold the low profile MW plates in the chamber ed eS gt 2 GS35 M 4x35 M 2x60 M GS60 M 1x60 M LABTEK 60 M For 1 1 x3 chamber slide and For 4 35 mm For 2 60 mm For 1 60mm petri dish and For 1 60 mm For 1 Lab Tek 1 x2 chambered 2 35mm petri dishes petri dishes petri dishes 1 1 x3 chamber slide petri dish cover glass and 1 60mm petri dish 1x35 M 6035 M 1xGS M 2xGS M 1xGS 2xLABTEK M 1xLABTEK M For 1 35 mm For 2 35 mm petri dishes and For 1 1 x3 For 2 1 x3 For 1 1 x3 chamber slide For 1 Lab Tek petri dish 1 60mm petri dish chamber slide chamber slides and 2 Lab Tek 1 x2 1 x2 chambered chambered cover glass gt glass LABTE2. H201 LUDL 96A602 User Manual March 2015 H201 LUDL 96A602 Compatible with the following Z piezo stage Compatible with the following Okolab Controllers Ludl 96A602 H201 T UNIT BL Page 1 www oko lab com H201 LUDL 96A602 User Manual March 2015 Index 1 Components and GIMENSIONS cecictsciesdeiateriaretesieees interes este eee aed 3 De Sat PleTIOVGeN Ss x tect ete coerce E E T tee ees eet ec eae ets 3 2 1 Available Sample HolderS essessessossossoesosossosesoesseosessesoesoesoesossoesoesoesoesossoesoesossoosossossossoosoo 3 de AVEDA E E E E E E NE E E EE E EEE 4 31 Koener Lideres re ree ree TS E E ET E E ne E 5 SL ecn EE a a E E E E E ears GeameeenGeeneesscessees 5 Cec a L ser Interlock Lids ee ee ee 6 4 Insertion of the Sample Feedback Temperature Sensor essessessossossossossosoosoosoosoosoosoosooeooesoeoseooo 7 5 Insertion of Sample Holder Into CHAMDEN siccieswescswsn eseesenuseeeesesw swan seessuecuensesweeuseeiesewebesnseseeeanboweans 7 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic LOCKS cece cceeccccecccesccccsccceeccs 8 7 Working with 1x3 and 1x2 chamber slides Magnetic lOCKS cece ccc ce ccc ccnccccecccceccceecccesccceeces 10 8 Working with MW Plates Magnetic locks and Chamber riser cccccccccccccccccccccccccecccecccecccecccecees 11 9 Connection of the Gas Supply ssesessesessecescoecescoeceecsecessecesoecescece
3. K 35 M LABTEK 60 M 1xLABTEK M 2xLABTEK M LABTEK II 35 M 2xLABTEK II M For 1 Lab Tek 1 x2 For 1 Lab Tek 1 x2 For 1 Lab Tek 1 x2 For 2 Lab Tek For 1 Lab Tek Il For 2 Lab Tek Chambered cover glass Chambered cover glass Chambered cover glass 1 x2 chambered 1 x2 chambered cover glass 1 x2 chambered cover glass and 2 35mm petri dishes and 1 60mm petri dish cover glass and 2 35mm petri dishes Figure 2 Available sample holders The following heated glass Lids are available for H201 LUDL 96A902 H201 KOEHLER LID For imaging under Koehler illumination Included in code H201 LUDL 96A902 H201 INJECTION LID Heated glass lid with two small openings sealed with flexible plastic allowing injec tion or permanent access to the sample Compatible only with sample holder 1x35 H201 LASER INTERLOCK LID Heated glass lid with safety switch Connects to laser controller and automati cally turns laser off when lid is lifted Compatible only with sample holder 1x35 OPTIONAL not included with H201 LUDL 96A602 www oko lab com Page H201 LUDL 96A602 User Manual March 2015 3 4 Koehler Lid The Koehler Lid is a heated glass lid and allows imaging under Koehler illumination The Koehler Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 3 shows chamber dimensions with Koehler lid with and without chamber riser H201 KOEHLER LID 97 Distance between focal plane and upper side of
4. amber ris er Magnetic locks hold MW plates in place into sample holder illustrated in Figure 14 NOTE Magnetic locks must be ordered separately NOT INCLUDED Product codes MW LOCK 22 5 and MW LOCK 16 5 Magnetic lock p 0 EEE MW 22 5 LOCK To be ordered separately Typically used for 6 12 24 48 MW MW 16 5 LOCK To be ordered separately Typically used for 96 MW Figure 14 Magnetic locks for MW plates To be ordered separately Chamber Riser increases chamber s height and is REQUIRED with 6 12 24 48 MW plates regardless of the Lid em ployed Figure 15 shows how to mount and screw the chamber riser Screws location is indicated by letter A Chamber Riser Figure 15 Riser Assembly www oko lab com bape 1 H201 LUDL 96A602 User Manual March 2015 9 Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H201 LUDL 96A9602 Silicon tubing connects to a gas input brass opening located on a corner of the H201 LUDL 96A902 See Figure 16 Connect by gen tly pushing silicon tubing onto brass opening Figure 16 Connection with gas supply 10 Working with Perfusion The Chamber Riser included with H201 LUDL 96A9602 features 12 perfusion holes for the insertion of perfusion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screws as
5. ncluded with sample holder Spacing rings accounting for variability in dish diameter from one manufacturer to another are also included Figure 9 shows how to position the ring Ring selection guide diagrams rings for 35 and 60 mm dish Figure 10 and Figure 11 respectively Figure 9 1 Insertion of the ring 2 insertion of the 35 mm dish 3 insertion of the magnetic lock NOTE Spacing rings are included with sample holder www oko lab com page H201 LUDL 96A602 User Manual March 2015 Grainer Petri35x10 Ibidi p Dish 35 mm low C oO Q BD Falcon 35x10 Willco 35x10 Corning 35x10 mm MatTek P35G X14 X Figure 10 Ring selection scheme for 35 mm dish eS Ibidi Dish 50 mm low MatTek P50G X30 X Q BD Falcon 60x10 Grainer Petri 60x15 Corning 60x15 mm Q Willco 60x15 Jof Figure 11 Ring selection scheme for 60 mm dish www oko lab com Page9 H201 LUDL 96A602 User Manual March 2015 7 Working with 1x3 and 1x2 chamber slides magnetic locks Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder Figure 13 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 page LO www oko lab com H201 LUDL 96A602 User Manual March 2015 8 Working with MW Plates Magnetic locks and Ch
6. necessary before introducing perfusion tubing Figure 17 shows location of perfusion holes Perfusion dedicated opening 1 Frontal view 2 3D view Figure 17 Perfusion www oko lab com el 2 H201 LUDL 96A602 User Manual March 2015 11 Connection of the Chamber with Z piezo stage H201 LUDL 96A602 fits on the Z piezo Stage without any screws To fix the chamber on the stage follow the steps shown in Figure 18 1 Place the chamber on the stage See Image 1 of Figure 18 2 Incline the chamber and slide it in direction of the springs until it snaps into the housing See Image 2 of Figure 18 3 Image 3 of Figure 18 shows the chamber fitted on the Z piezo Stage Figure 18 Connection of the Chamber with Z piezo Stage www oko lab com bape 3
7. soecescececoecesoecesoecessesesoeseecoeceese 12 10 Working with PENTUSION vec cvinedsievsaslssaesasbesewesc a a a a a a d e aa 12 11 Connection of the Chamber with Z piezo stage ssesescecescoecescoecesoecesoecesoecesoecesosceseeceseeceseeceseeceo 13 www oko lab com es H201 LUDL 96A602 User Manual March 2015 1 Components and dimensions H201 LUDL 96A602 includes the following components Chamber main body Glass lid for working under Koehler illumination Chamber riser It is a removable frame increasing the height of the chamber from 21 to 27 mm often re Distance between focal plane 27 and upper side of the glass lid with the chamber riser Distance between focal plane 21 and upper side of the glass lid without the chamber riser Sample Holder Order Separately Chamber Base O r Or Chamber top view gt quired when using multi well MW plates H201 KOEHELER LID Chamber Riser Figure 1 H201 LUDL 96A902 Components and Dimensions 2 Sample Holders 2 1 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1 35mm Petri dish 1 1x3in chamber slide 1 Lab Tek 1in x2in chambered cover glass 1 Lab Tek II 1in x2in chambered cover glass 1 60mm Petri dish 1 Nunc and
8. the glass gt lid with the chamber riser Distance between focal plane 21 and upper side of the glass lid without the chamber riser Figure 3 H201 LUDL 96A902 H201 KOEHLER LID 3 2 Injection Lid The Injection Lid is a heated glass lid with two small openings sealed with flexible plastic allowing injection or perma nent access to the sample NOTE Compatible with sample holder 1x35 ONLY The Injection Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 4 shows chamber dimensions with the Injection Lid with and without chamber riser www oko lab com pee H201 LUDL 96A602 User Manual March 2015 28 Distance between focal plane and upper side of the glass lid with the chamber riser 0 Distance between focal plane 22 and upper side of the glass lid without the chamber riser 0 Chamber top view Figure 4 H201 LUDL 96A902 H201 INJECTION LID 3 3 Laser Interlock Lid The Laser Interlock Lid is a heated glass lid with a safety switch connecting to the laser controller It automatically turns the laser off when the lid is lifted NOTE Compatible with sample holder 1x35 ONLY The Laser Interlock Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 5 shows chamber dimensions with the Laser Interlock Lid with and without chamber riser H201 LASER INTERLOCK LID Distance between focal plane 2 and upper side of the glass lid with the chamber riser Distance bet
9. ween focal plane 23 and upper side of the glass lid without the chamber riser Chamber top view 109 70 s Figure 5 H201 LUDL 96A902 H201 LASER INTERLOCK LID www oko lab com H201 LUDL 96A602 User Manual March 2015 4 Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H201 LUDL 96A602 see Figure 6 Frontal and 3D views Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1 Frontal view 2 3D view Figure 6 Insertion of the temperature sensor inside the chamber 5 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Figure 7 Figure 7 Introduction of the Specimen Holder inside the Chamber Base www oko lab com ew H201 LUDL 96A602 User Manual March 2015 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder Figure 8 illustrates the available magnetic locks for 35 and 60 mm dishes Threaded magnetic posts allow adjusting holder s height Figure 8 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are i
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