Pipet-Lite XLS Multichannel with RFID Manual
Contents
1. 6200 133 SL 10 XLS 6200 138 6200 139 6200 140 6200 133 SL 20 XLS 6200 143 6200170 6200 145 6200 144 a SL 100 XLS 6200 150 6200 151 6200 147 6200 148 SL 200 XLS 6200 154 6200 155 6200 157 6200 156 SL 300 XLS 6200 415 6200 414 6200 413 6200 419 SL 1000 XLS 6200 161 6200 162 6200 160 6200 163 SL 2000 XLS 6200 166 6200 167 6200 169 6200 168 Models Piston Cylinder Shaft Tip Ejector Arm gt 5000 wl O Ring O Ring L 5000 XLS 6200 363 6200 364 6202 222 6200 373 ee L IOML XLS 6200 369 6200 370 6202 223 6200 374 L 20MLXLS 6202 299 6202 300 6202 302 6202 298 tha SL 5000 XLS 6200 363 6200 364 6200 362 6200 373 mn SL 10ML XLS 6200 369 6200 370 6200 368 6200 374 SHAFT EJECTOR ARM Figure 8 Common Replacement Parts Top lt 2000 uL Bottom gt 5000 uL 2 1 RFID Radio Frequency Identification Device All Pipet Lite XLS models incorporate a passive RFID tag in the finger hook containing unique information about the pipette including model type serial number RFID UID and service cali bration information The RFID tag can be wirelessly linked with optional reader and software to facilitate querying calibration tracking and service reminders The RFID tag has negligible weight with no effect on precision or accuracy It operates only when read by the RFID reader and the below me2. 2 uL 0 1 2uL 1 2 mm 10 uL 0 5 10 uL 1 2 mm 20 uL 2 20 uL 2 3 mm 100 uL 10 100 uL 2 3 Mmm 200 UL 20 200 UL 3 6mm 300 uL 20 300 UL 3 6mm 1000 uL 100 1000 pL 3 6mm 2000 pL 200 2000 uL 3 6mm 5000 uL 500 5000 uL 6 10mm 10 mL 1 mL 10mL 6 10mm 20 mL 2 mL 20 mL 6 10mm always be used in a position within 20 degrees of vertical See Figure 6 below TIP IMMERSION DEPTH Figure 6 Tip Immersion Depth and Tip Angle 1 5 Boa R ena 1 6 Operation Before pipetting valuable samples it is good to practice aspirating and dispensing water Set the desired volume as described on page 4 Attach a new Rainin tip Press the shaft into the tip with only sufficient force to make a good seal Press the plunger button to the first stop and hold it in this position Aspiration Holding Pipet Lite XLS vertically or within 20 of vertical place the tip into the sample to the proper depth and relax your thumb pressure on the plunger The light piston spring will move the piston upward aspirating sample Pause for about 1 second longer for macro volume pipettes to ensure that the full volume of sample is drawn into the tip Withdraw the tip from the sample If any liquid remains on the outside of the tip touch it off carefully onto a lint free tissue taking care not to touch the tip orifice Dispensing Touch the tip end against the side wall of the receiving vessel and press the plunger slowly past
3. 614 153 5 700 959 and 5 849 248 For use under U S patents 6 168 761 B1 6 171 553 B1 and D426 643 2010 Rainin Instrument LLC No part of this manual may be copied without the express written permission of Rainin Instrument LLC 1 1 Introduction Rainin s new Pipet Lite XLS is based upon Pipet Lite and incorporates several new features to further improve ergonomics while maintaining high accuracy and per formance Pipet Lite XLS is available in several versions outlined below This manual will describe all models Single channel 11 models for volumes from 0 1 ul fo 20 ml Multichannel 12 models for volumes from 1 ul to 1200 ul including 8 and 12 channels Adjustable spacer 5 models for volumes from 5 ul to 1200 ul including 6 and 8 channels with nozzle spacing adjustable from 9 19 or 9 14 mm Spring forces in the Pipet Lite XLS are reduced even further over the already low forces used in Pipet Lite with the low drag seals new contoured handle with fingerhook the pipette is designed for maximum comfort and enhan ced performance not compromised by fatigue over long pipetting days A magnet is used fo help locate and hold Pipet Lite XLS piston in the zero or home position which defines the starting point for the pipette cycle and allows the use of lighter aspiration and blowout springs reducing forces on the operator s thumb In models with LTS shafts L model single channels all multichannels and adjus
4. Spacer Pipette l Figure 15 Liquid End Marks The full range of adjustment for the eight channel version is shown below in Figure 17 Nozzles are set fo 9 mm spacing on the left and 14 mm spacing on the right Figure 16 Nozzles minimum spacing amp maximum spacing Tip Selection Pipet Life multichannel pipettes must use Rainin LTS tips LA8 50 use 250 ul LTS tips LA6 300 and LA8 300 use 300 ul LTS tips LA6 1200 and LA8 1200 use 1200 ul LTS tips recommended or 1000 ul LTS tips picks up 1200 ul except tips with filters LTS tips have a cylindrical cross section with a well defined seal ring thin wall and positive stop They seal properly on the LTS nozzles and cannot be jammed or forced too far onto the nozzles Mounting LTS Tips Mounting racked LTS tips onto LA6 and LAS pipettes is simple Set the nozzle spacing to 9 mm same as the spacing for racked tips by rotating the dark grey SPACING ADJUSTMENT knob fully clockwise Align the nozzles into the row of tips holding the pipette at a slight angle Figure 17 left Rotate the pipette upright and press the nozzles into the tips until the positive stop is reached The tips are now mounted with proper sealing You do not need to hand tighten use heavy pressure or rock the nozzles onto the tips to obtain a good seal Withdraw the tips from the tip rack Figure 17 right Figure 17 Mounting LTS Tips Setting the Nozzle Spacing With tips m
5. pipettes amount of grease Cracked or split shaft Replace shaft Check piston is not bent If bent call Rainin service for a new piston 800 543 4030 in the US Rough jerky or Sample splash inside mechanism leading Remove tip ejector arm remove shaft and sticky plunger to staining and or corrosion check piston If piston is corroded or stained movement call Rainin service for a new piston 800 543 4030 in the US When removing the shaft from the pipette body make sure the spring seal and o ring do not detach from the piston especially on the smaller models Be careful not to bend the piston on these small models Recalibration of Pipet Lite XLS is only required when the piston is replaced Service Calibration and Repair It is recommended to use only genuine Rainin replacement parts such as seals o rings and shafts It is NOT necessary to recalibrate the pipette after changing the seal o ring or shaft Recalibration of the pipette is only necessary when the piston is replaced and should be done only by qualified factory trained personnel in a Rainin approved facility For pipettes under warranty please note that the warranty will be voided if the pipette has been damaged as a result of physical or chemical abuse or if the pipette has been repaired or recalibrated by any service facility which is not authorized by Rainin In the US call 800 543 4030 for service Service is also available outside the U
6. pll0 75 ml 1 25 ml 4 25 ml 7 5 ml 12 5 ml Figure 3 Volume Indicator by Pipette Model Read the volume indicator from the top down 2 20 pL Black digits indicates pl Red digits tenths hundredths of ul 100 300 ul All digits black pl 1000 5000 ul red digit ml Black digits tenths hundedths of ml 10 ml and 20 ml Red digit ml Black digits tenths of ml Turn the volume lock fo the lock position Fig 2 above fo prevent changes to the volume setting Some volumes for the 200 uL model are shown in Figure 4 67uL py 108 uL Figure 4 Example volumes Volume ranges and increments for each Pipet Lite XLS model are shown below Single Channel Pipet Lite XLS Volume Adjustable Range yL Recommended Range uL Increment uL 2 uL 0 to 2 0 1 to 2 0 002 10 pL Oto 10 0 5 to 10 0 02 20 uL 0 to 20 2 to 20 0 02 100 uL O to 100 10 to 100 0 2 200 uL O to 200 20 to 200 0 2 300 pL O to 300 20 to 300 0 5 1000 UL 0 to 1000 100 to 1000 2 0 2000 uL O to 2000 200 to 2000 2 0 5000 uL 0 to 5000 500 to 5000 5 0 10 mL Oto 10 mL 1 mL fo 10 mL 20 0 20 mL O to 20 mL 2 mL fo 20 mL 20 0 Multichannel and Adjustable Spacers Volume Adjustable Range yL Recommended Range L Increment uL 10 uL Oto 10 0 5 to 10 0 02 20 uL 0 to 20 2 to 20 0 02 50 uL O to 50 5 to 50 0 05 100 uL O to 100 10 to 100 0 2 200 uL O to 200 20 to 200 0 2 3
7. 0 6 500 0 8 4 0 0 2 1 0 1000 0 8 8 0 0 15 1 5 1200 uL 100 2 3 6 3 6 0 6 0 6 600 0 8 4 8 0 2 1 2 1200 0 8 9 6 0 15 18 2000 uL 200 2 3 0 6 0 0 6 1 2 1000 0 8 8 0 0 2 2 0 2000 0 8 16 0 0 12 24 5000 uL 500 5 24 12 0 0 6 3 0 2500 0 6 15 0 0 2 5 0 5000 0 6 30 0 0 16 8 0 lOmL lImL 20 5 0 50 0 0 6 6 0 5 mL 1 0 50 0 0 2 10 0 10 mL 0 6 60 0 0 16 16 0 20mL 2mL 20 5 0 100 0 0 6 12 0 10 mL 1 0 100 0 0 2 20 0 20 mL 0 6 120 0 0 16 32 0 Specifications are subject to change without notice Multichannel models only in these volume ranges 1 12 Replacement Parts The commonly replaced parts are shown here for each volume range for Pipet Life XLS single channel LTS and universal fit models The images next to the tables represent the 200 ul model top and the 10 mL model bottom other models are similar Models Seal O Ring Shaft Tip Ejector Arm lt 2000 yl L 2 XLS 6200 131 6200 132 6202 063 6202 071 L 10 XLS 6200 138 6200 139 6202 064 6202 071 L 20 XLS 6200 143 6200 170 6202 065 6202 071 L 100 XLS 6200 150 6200 151 6202 066 6202 073 L 200 XLS 6200 154 6200 155 6202 067 6202 073 L 300 XLS 6200 415 6200 414 6202 425 6200 419 N L 1000 XLS 6200 161 6200 162 6202 068 6202 074 O RING L 2000 XLS 6200 166 6200 167 6202 214 6200 168 SL 2 XLS 6200 131 6200 132 6200 134
8. 00 uL 0 to 300 20 to 300 0 5 1200 uL O to 1200 100 to 1200 2 0 Filter Single Channel Pipet Life XLS 5000 uL 10 mL and 20 mL pipettes use a filter in the end of the shaft to help prevent liquid entering the shaft and contaminating the piston should the plunger snap up during aspiration Using a filter is recommended when pipetting large volumes Replace the filter if it gets wet END OF END OF END OF 5000 pL SHAFT 10 mL SHAFT 20 mL SHAFT WU H H Q FILTER FILTER Figure 5 Filter orientation The 5000 uL and 10 mL pipettes use the same filter as shown in the diagram above 5000 pL small diameter into the shaft 10 mL large diameter into the shaft Filter part numbers are 6190 164 pack of 100 and 6190 165 pack of 1000 The filter for the 20 mL model is a cylinder Part numbers 6190 221 pack of 100 6190 222 pack of 500 1 3 1 4 Tip Selection and Mounting Rainin pipettes and tips are designed together as a pipetting system both in LTS and traditio nal versions All Rainin tips are BioClean and totally inert so you can be assured of the best pipetting results Pipet Life XLS pipettes are calibrated with Rainin tips and performance to published specifica tions can only be guaranteed when Rainin tips are used Tip immersion Depth The recommended depth for tip insertion into the sample for each model is shown below Nominal Volume Volume Range Immersion Depth
9. RAININ Pipet Lite XLS Manual Pipettes with RFID Single channel Multichannel Adjustable Spacer U Ca a ees O i aoa Y C arees O h aa aD D Pipet Lite XLS Manual Pipettes with RFID e Adjustable spacer multichannel models with LTS e RFID anabled all models Tip Ejector Arm Removal ccceeeecccceeteceectteeeeesstteeeessttieeessctteeeesstsieeeessnieeeees 8 Pipe tte XLS SOP GS e tina teenstendsaisasduueladak Machadeen thin sata A mutadudaraeattts 8 TFOUBIESNOOTIG n E E E N E A E 8 Service Calibration and Repdit cissncassiesesavessedssancecaganeaeasadednnedarsadbianavs sgadeanastenante 9 SPOCIICONONS ssis odininkai oi a aii en e a a a shallots 9 Replacement PANS criin aa a a a aa Eea aaRS 11 REID OPSTON Oliiaiesnmionr niani doe eE E E EEA EN 12 Pipet Lite XLS MUITICNONNEL 0 0 ccc cccccccccececesesesseaeeeseeeeeeseesssesseasaaeeeeesenees 14 Pipel Lite XLS Adjustable Spacon s sssccesiiuesanndstwunadaed asada del E EE 16 Contents of Pipet Lite XLS box e Pipet Lite XLS model as ordered e CD ROM containing this manual and trial RFID software e Sample Tips e Conformance Certificate Warranty Card If any item is missing please call 800 472 4646 in the US or contact your local MT office or distributor Rainin Pipet Lite XLS LTS LiteTouch Hang Ups are trademarks of Rainin Instrument LLC Pipet Lite pipettes are manufactured under U S Patent Nos 5
10. S See www mt com rainin for more information Acids and Corrosives Extensive contact with corrosive fumes may result in premature seal wear and damage to the piston Exposure of the internal components to corrosive aerosols and fumes may be reduced by using Rainin tips with aerosol barrier filters After using concentrated acids or corrosive solutions you can disassemble Pipet Lite XLS and inspect and clean the piston assembly shaft and seal o ring with distilled water Use extreme care on the 2 ul and 10 ul models to avoid damaging the small diameter piston or losing small items such as seals Dry all components thoroughly and reassemble 1 11 Specifications These manufacturer s specifications should be used as guidelines when establishing your own performance specification Volume Increment Accuracy Precision Model pL pL pL pL s 2 uL 0 2 0 002 12 0 0 024 6 0 0 012 1 0 2 7 0 027 1 3 0 013 2 0 1 5 0 030 0 7 0 014 10 uL 1 0 0 02 2 5 0 025 1 2 0 012 5 0 1 5 0 075 0 6 0 03 10 0 1 0 0 1 0 4 0 04 20 uL 2 0 02 7 5 0 15 2 0 0 04 10 1 5 0 15 0 5 0 05 20 1 0 0 2 0 3 0 06 50 uL 5 0 05 3 5 0 18 1 5 0 075 25 1 2 0 3 0 4 0 1 50 0 8 0 4 0 2 0 1 100 uL 10 0 2 3 5 0 35 1 0 0 1 50 0 8 0 4 0 24 0 12 100 0 8 0 8 0 15 0 15 200 uL 20 0 2 2 5 0 5 1 0 0 2 100 0 8 0 8 0 25 0 25 200 0 8 1 6 0 15 03 300 uL 30 0 5 2 5 0 75 1 0 0 3 150 0 8 1 2 0 25 0 375 300 0 8 2 4 0 15 0 45 1000 uL 100 2 3 0 3 0 0 6
11. Windows XP Vista amp Windows 7 Users can select any of the following languages Chinese Simplified Czech Danish English French German Hungarian Italian Japanese Korean Norwegian Polish Russian Spanish Swedish Operation Overview An optional RFID Kit is available for purchase from Rainin The RFID Kit contains the Rainin recommended RFID reader and the LabX Direct Pipette Scan software Once the Rainin RFID reader is connected by USB to your PC and the software is configured and running operation is simple Simply place the head of the pipette in the recommended position as shown on the Rainin RFID reader hold it steady for a few seconds to scan the pipette s RFID tag and read the pipette s Figure 9 information stored in its RFID tag into the software Rainin RFID Reader Read only fields from the Pipette s RFID Tag A eee eee EN mala Pipette Data Read factory data from pipette and read write user defined data RFID UID Factory Date Model Number Last Service Serial Number Next Service Pipette Data Define User Fields Transfer Data Format Options Leo Figures 10 and 11 Pipette Data Read factory data from pipette and read write user defined data Screen shots of the software caus i i RFID UID 7D87DE94 Factory Date 5 20 2010 A before the pipette IS scanned Model Number L 200 LS Last Service 5 20 2010 B after the pipette is scanned Serial Number ENGROOOIS Next Service 5 20 2010 The so
12. and pull the ejector down For 5000 uL and 10 mL models grasp the top of the ejector arm and pull outward then downward For the 20 mL model pull off the lower part of the ejector arm the upper part stays in place Replace by aligning the rod with the hole in the upper part and pressing firmly To replace the ejector arm except 20 mL insert the shaft through the large opening align the top with the tip ejector push rod and push until the ejector arm snaps in place lt 2000 uL 5000 uL 10 mL 20mL gt Figure 7 Removing Tip Ejector Arm Pipet Lite XLS Storage After use store the pipette in a clean safe place Pipet Lite XLS is a precision instrument and should be treated with the level of care appropriate for laboratory instrumentation Three types of hanger are available to hold your Pipet Lite XLS conveniently when not in use CR 7 Free standing carousel holds seven pipettes HU M3 Set of three individual magnetic Hang Ups for mounting on ferrous surfaces HU S3 Three Hang Ups attached to a clamp which fits onto a shelf 1 9 1 10 Troubleshooting Problem Possible Cause Suggested Remedy Leaks Loose shaft Tighten coupling nut by hand inaccuracy Worn seal or o ring Replace seal and o ring pipette volumes lt 2000 ul Worn o ring or for pipette volumes 5000 Replace o ring and for 5000 ul ul 1Oml 20 ml insufficient grease seal 10 ml and 20 ml pipettes only apply small on these
13. ftware shows the read only data fields programmed into each pipette s RFID tag before it leaves the factory These fields can ONLY be modified by Rainin when the pipet is manufac tured or updated when sent in for service The read only fields include the RFID UID Model Number Serial Number Factory Date Last Service Date and Next Service date For QC or workflow purposes multiple RFID enabled pipettes can be scanned sequentially Writing Data to the RFID Tag s custom fields In addition there are writable fields available on each pipette s RFID tag that can be chosen by the users to customize their pipettes so as to standardize their unique workflow s Each customer is free to customize and or standardize the software to suit their unique workflow s Any such writing operation requires the Rainin RFID reader and the LabX Direct Pipette Scan software Refer to the software help file for more instructions What RFID LabX Direct Pipette Scan Software can do The LabX Direct Pipette Scan software is flexible to help you customize your workflow Customers can standardize their departments on the new Pipet Life XLS pipettes and Rainin RFID reader with LabX Direct Pipette Scan software system based workflow and calibration check system This systems helps simplify the work of Quality Compliance Specialists It shortens the calibration check cycle returning pipettes back to active lab use faster In addition data can also be written to the Pipe
14. hannel models and operate in the same way Two versions of the Pipet Lite XLS Adjustable Spacer pipette are available 6 channel LA6 and 8 channel LA8 with nozzle spacing adjustment ranges shown below 6 channel 9 mm fo approx 19 mm 8 channel 9 mm fo approx 14 mm 6 and 8 channel versions are available in the volume ranges 20 300 uL and 100 1200 pL 8 channel versions are also available in the 5 50 uL volume range Spacing Controls and Indicators Nozzle spacing is changed with the use of two knurled knobs on either end of the liquid end mani fold the LIMITER knob and the SPACING ADJUST MENT knob shown in Figure 14 The LIMITER knob marked with the nozzle spacing range sets the desired maximum spacing within the pipette s spacing range A vertical arrow at the top of the knob on the liquid end manifold is the set point shown in Figure 15 When you have set the maximum spacing with the LIMITER knob turn the SPACING adjustment knob to open the nozzle spacing to the set value A scale on the liquid end manifold gives a visual reference of the set spacing Simply line up the the leftmost nozzle with the scale on the manifold as shown in Figure 15 PLUNGER BUTTON VOLUME SET VOLUME LOCK TIP EJECTOR BUTTON RFID IN FINGER HOOK VOLUME DISPLAY SS o LIQUID END MANIFOLD LIMITER KNOB Ry L SPACING ADJUSTMENT KNOB LTS DISPOSABLE TIPS Figure 14 Pipet Lite XLS Adjustable
15. ntioned software Optional Rainin RFID Reader Rainin recommends a specific Rainin RFID reader that interfaces with a PC s USB port Rainin does not recommend any other RFID reader Using the Rainin RFID reader information can be written to the Pipette s RFID tag in the same consistent way as it is read from the RFID tag The Rainin RFID Reader can be used with any Rainin RFID enabled Pipet Lite XLS but needs appropriate software for it to work The estimated range of operation of the RFID reader is up fo 5 cm How to read the Rainin RFID Reader LEDs Light emitting diodes LEDs Description Yellow Reader powers up in this state Reverts to this state when application is stopped amp Green or a plug in is disabled Yellow Reader has detected a pipette Keep pipette close to reader until LED changes to Green or Red Green When application first starts Green LED indicates the device is ready After all data is read from a pipette held close to the reader Green LED lights up Red Reader failed to read or write Try scanning the pipet again if not restart software Yellow Data written to the pipette does not compare with data read back from the amp Red pipette Optional LabX Direct Pipette Scan Software Supplied on the same CD as this manual is a trial version of the Lab X Direct Pipette Scan software A full version is also available for purchase Supported PC Operating Systems Microsoft
16. ounted on the nozzles and before you begin to aspirate sample e f you know the spacing for the receiving wells or test tube block set this value on the LIMITER knob e f you do not know the spacing hold the pipette so that the tip ends are above the centers of the wells or test tube block into which you will dispense Look at the scale on the liquid end manifold and note where the marked nozzle aligns Set this value on the LIMITER knob e f you accidentally over extend the spacing you can click the LIMITER knob to smaller values stopping when the tip ends are aligned over the centers of the wells test tubes Once the LIMITER knob has been set spacing the nozzles properly is simple Just pick up sample set the nozzle spacing by moving the SPACING ADJUSTMENT knob until it stops at the value set by the LIMITER knob and dispense into the wells or test tube block Tip Immersion Depth Recommended depth for tip insertion for each Pipet Lite XLS adjustable nozzle model LA8 50 2 3 mm LA6 300 LA8 300 LA6G 1200 LA8 1200 3 6 mm Operate the pipette within 20 degrees of vertical Tip Ejection Simply press on the tip ejector button All tips are ejected cleanly with minimal pressure on the thumb because of the progressive eject design built in to the liquid end manifold Positioning the Liquid End Manifold The liquid end manifold can be rotated to any angle for convenience You do not need to loosen the coupling nut Rainin In
17. positive stop They seal properly on the shaft and cannot be jammed or forced too far onto the shaft nozzles and provide absolutely consistent sample pickup across all channels Mounting LTS Tips 8 and 12 channel PLUNGER BUTTON VOLUME SET VOLUME LOCK TIP EJECTOR RFID IN BUTTON FINGER HOOK VOLUME DISPLAY od LIQUID END MANIFOLD My LTS DISPOSABLE TIPS Figure 12 Pipet Lite XLS Multichannel Pipette Mounting racked LTS tips on L8 and L12 pipettes is simple First align the shaft nozzles into the row of tips holding the pipette at an angle Figure 13A Position the pipette upright and press the nozzles into the tips until the positive stop is reached Figure 13B The fips are now mounted with proper sealing You do not need to hand tighten use heavy pressure or Figure 13 Mounting racked LTS Tips L8 and L12 Tip Immersion Depth The recommended depth for tip insertion is shown in the table below Pipet Lite XLS Model Immersion Depth L8 10 L12 10 L8 20 L12 20 2 3mm L8 200 L12 200 L8 300 L12 300 L8 1200 L12 1200 3 6mm Positioning the Liquid End Manifold The liquid end manifold can be rotated to any angle for convenience when filling plates You do not need to loosen the coupling nut Pipet Lite XLS Multichannel Pipette with Adjustable Spacing 6 and 8 channel models Introduction Pipet Lite XLS Adjustable Spacer multichannel pipettes are based on Pipet Lite XLS single c
18. strument LLC 7500 Edgewater Drive Oakland CA 94621 Sales 1 510 564 1600 Service 1 510 564 1600 Subject to technical changes 2010 Rainin Instrument LLC Printed in USA 9920 386 Rev E a METTLER TOLEDO Company Wwww rainin com For more information
19. table spacers the patented LTS LiteTouch Tip Ejection System reduces tip ejection forces by up to 85 in the single channel L models and provides absolutely consistent sample pickup across all channels in multichannel models as well as reducing tip ejection force Finally all Pipet Lite XLS models contain an RFID radio frequency identification for facilitating calibration management when using the optional RFID reader and Lab X Direct Pipette Scan software Using the RFID is described after the single channel section PLUNGER BUTTON VOLUME LOCK VOLUME SET TIP EJECTOR BUTTON RFID IN FINGER HOOK VOLUME DISPLAY RUGGED HIGH IMPACT LOW THERMAL CONDUCTIVITY POLYMER TIP EJECTOR QUICK RELEASE i STAINLESS STEEL SHART TIP EJECTOR ARM DISPOSABLE TIP Figure 1 Pipet Lite XLS 1 2 Setting Volume Turn the volume lock lever to the unlock position so the volume setting mechanism is un locked and free fo turn See left image in Figure 2 UNLOCK LOCK Figure 2 Volume Lock Control With the mechanism unlocked orient Pipet Lite XLS so you are looking at the volume indica tor then rotate the plunger button to change volume counter clockwise to increase and clockwise to decrease volume 2p 10 pl 20 ul 100 pl 200 pl 300 pl 1000 pl 2000 pl 5000 ul 10 ml 20 mi 1 0 1 0 1 2 0 1 4 0 1 2 7 2 7 2 2 7 2 2 7 2 5 5 5 5 5 5 5 5 5 5 5 4 25 pl 7 5 pl 12 5 pl 75 pl 125 pl 225
20. the first stop to blowout bottom of stroke Wait 1 second for 2 300 pL volumes 1 2 seconds for 1000 uL and larger For viscous solutions pause before blowout Still holding the plunger withdraw the tip sliding it along the wall of the vessel Release the plunger Press the tip ejector button lightly to discard the tip Use a new tip for each sample to prevent carry over Repeat for the next pipetting cycle Pipetting Guidelines Pipet Lite XLS pipettes incorporate several features to enhance pipetting consistency You should also maintain Consistent pickup and dispense rhythm Consistent speed and smoothness when pipetting Consistent pressure on the plunger button at the first stop Consistent immersion depth See Figure 6 Pipette vertically or within 20 of vertical Pre rinse the tip twice by aspirating and dispensing sample before actual pickup More information on Good Pipetting Technique including a Lab Poster can be found on the Rainin and MT websites www rainin com or www mt com rainin Autoclaving Autoclavable parts of Pipet Life XLS are the shaft and the tip ejector 121 C 1 bar 15 20 minutes DO NOT autoclave the complete pipette or any parts other than the shaft and the tip ejector 1 7 1 8 Tip Ejector Arm Removal Three types of tip ejector are used and all types can be removed with minimum effort do not use force For models up to 2000 uL press in the quick release tabs on the ejector arm
21. tte s RFID tag by configuring the custom fields in the LabX Direct Pipette Scan software and advanced workflows can be defined for your custom settings What RFID does not do e Does not protect against misplacement theft or provide pipette s location information e Does not protect against erroneous data input e Does not replace calibration stickers for businesses or displace QC professionals e Does not validate your workflow The validation of the workflow and CFR Part 11 regulatory compliance remains the responsibility of the customer 3 1 Pipet Lite XLS Multichannel Pipettes 8 and 12 channel models Introduction Pipet Lite XLS multichannel pipettes are based on the Pipet Life XLS single channel pipette and use the patented LTS LiteTouch Tip Ejection System Two versions of the Multichannel Pipet Lite XLS are available 8 channel and 12 channel An 8 channel version is shown in Figure 12 The 8 and 12 channel models are available in 6 volume ranges 0 5 10 uL 2 20 uL 5 50 uL 20 200 uL 20 300 uL 100 1200 L Tip Selection Pipet Lite XLS multichannel pipettes use Rainin LTS tips L8 10 L8 20 L12 10 L12 20 20ul LTS tips L8 50 L12 50 L8 200 L12 200 250 ul LTS tips L8 300 L12 300 300 ul LTS tips L8 1200 L12 1200 1200 ul LTS tips recommended or 1000 ul LTS tips pick up 1200 ul except with filter LTS tips have a cylindrical cross section with a well defined seal ring thin wall and
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