Multipurpose Mini Spin Columns
Contents
1. BioVision FOR RESEARCH USE ONLY Multipurpose Mini Spin Columns 03 14 Store at Room Temperature Cat No 6572 50 50 Mini Spin Columns Salient Features Column Material Polypropylene FRIT Material Polyethylene PE Porosity 20 um Bed Volume 700 ul Description Protein activity and functional studies rely largely on the availability of purified proteins BioVision s Multipurpose Mini Spin Columns offer a convenient option to purify target proteins by multiple small scale laboratory techniques Along with affinity purification and recombinant protein purification these columns can also be used for techniques like immunoprecipitation desalting etc These spin columns are constructed of polypropylene with a polyethylene FRIT They have a porosity of 20 um and a total bed volume of 700 ul Each of these columns can be packed with a variety of media beads for centrifugation based separation of biomolecules The columns are equipped with tight top screw caps and a bottom plugs making them ideal also for storage of beads media in buffers incubation of beads with biological samples etc Selected Applications e Immunoprecipitation and co immunoprecipitation with protein A G beads Cat 6501 6503 6511 6513 6520 etc Antibody purification using protein A G L beads Cat 6501 6503 6511 6513 6520 6531 6541 etc Affinity protein purification with beads like the EZEnrich Polyubiquitin Beads Cat 6568 Protei2. lose both the top cap and the bottom plug and place column in a 1 5 ml tube Heat the tube in a 100 C heating block for 5 min to elute the proteins bound to the beads Open the top cap first before removing the bottom plug 12 Place the column in a 1 5 ml microcentrifuge tube and centrifuge to collect the eluate in the tube Repeat the elution steps if needed N RELATED PRODUCTS Hi Bind Ni QR Agarose Beads 6562 10K Spin Column 1997 Heparin Sepharose 6553 Heparin Sepharose Column 6554 Glutathione Sepharose 6555 Jacalin Sepharose 6561 Ready to use Ni QR Agarose Beads Buffer Kit K6563 3 Hi Bind Protein A Agarose 6520 Protein A Agarose 6526 Protein A Sepharose 6501 Protein A Sepharose Column 6508 Hi Bind Protein G Agarose 6513 Protein G Sepharose 6511 Protein G Sepharose Column 6518 Protein L Sepharose 6531 Protein L Sepharose Column 6538 Protein A G Sepharose 6503 Protein A G Sepharose Column 6528 Protein A G L Sepharose 6541 Protein A G L Sepharose Column 6548 FOR RESEARCH USE ONLY Not to be used on humans 155 S Milpitas Blvd Milpitas CA 95035 USA T 408 493 1800 F 408 493 1801 www biovision com tech biovision com
3. n DNA desalting using Sephadex G25 or Sephadex G50 Recombinant protein affinity purification of proteins with polyhistidine tag GST tag etc General Procedure Note This is a reference procedure for affinity purification Modifications may be required based on the application The centrifugation speeds depend on the bead type Please follow the manufacturer s recommendation for best results 1 Snap off the bottom plug of the column and save it for later use to plug the column during the elution step 2 Open the top screw cap and place the column in a 2 0 ml microcentrifuge tube 3 Load your resin beads packed bead volume 10 500 ul and spin it down once to remove the suspension solution 4 Add the equilibrium buffer centrifuge and discard the solution Repeat this step twice if needed 5 Close the column with the bottom plug saved in step 1 6 Carefully apply the sample to the column close the top screw cap and mix end over end to incubate the sample Slightly loosen the top cap remove the bottom plug and place the column in a microcentrifuge tube Centrifuge once to collect the flow through 8 Place the column in another microcentrifuge tube add the washing buffer centrifuge and discard the solution Repeat the washing as needed 10 Close the bottom using the plug add the elution buffer and tap the column gently to mix the beads and the elution buffer 11 Optional If boiling is required to elute the target protein c
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