GEP 077 Beckman SW-55 Ti Rotor
Contents
1. THE UNIVERSITY OF NEWCASTLE DISCIPLINE OF MEDICAL BIOCHEMISTRY STANDARD OPERATING PROCEDURE PROCEDURE NO GEP 077 MOD 1st Issue Page 1 of 3 Procedure Type General Equipment Procedure Title Beckman SW 55 Ti Swinging Bucket Rotor 1 Risk Assessment This Risk Assessment is to be used as a general guide and as such cannot accommodate all the varying factors that may be encountered when using this equipment Therefore personnel are requested to conduct their own Risk Assessment before using this equipment to include any extra hazards introduced by the task performed TASK PERFORMED 1 Centrifugation in the Beckman L8 70M Preparative Ultracentrifuge HAZARDS 1 Rotor imbalance leading to machine destruction and possible injury to those in the vicinity 2 Rotor fracturing during operation 3 Exposure to biohazards hazardous substances as a result of broken leaking vessels 4 Use of incorrect buckets with rotor RISK ASSESSMENT 1 The risk of machine imbalance is present while ever the equipment is in use These risks are increased if the user is not trained or if the centrifuge is not regularly maintained 2 The risk of the rotor breaking during operation is low if the rotor has been properly maintained 3 Risk of exposure to biohazards due to broken leaking vessels is present while ever the machine is in use 4 The risk of use of incorrect buckets can be minimized by training an2. d labelling RISK CONTROL Only staff trained specifically in the use of this centrifuge are to use this equipment Training is to be recorded in Staff training folder and names of authorized personnel are to be displayed on the wall in the centrifuge room 1 1 Tubes must be balanced by weight before inserting into the buckets NAME signed Lynn Herd Adam Odell Phil Dickson DATE 13th December 2004 28th February 2005 28th February 2005 Distributed To GEP Master File GEP Lab File THE UNIVERSITY OF NEWCASTLE DISCIPLINE OF MEDICAL BIOCHEMISTRY STANDARD OPERATING PROCEDURE PROCEDURE NO GEP 077 MOD 1st Issue Page 2 of 3 Procedure Type General Equipment Procedure Title Beckman SW 55 Ti Swinging Bucket Rotor 1 2 Only tubes especially made for centrifugation are to be used 1 3 Establish a safety zone of 30cm around the centrifuge and ensure that no personnel or hazardous substances enter the zone during centrifugation 2 1 Clean rotor thoroughly after each use 2 2 Store upside down in a cool dry area when not in operation 2 3 Check rotors regularly for signs of irregular wear damage 2 4 The rotor should be checked by the manufacturer on a regular basis for pitting or hairline fractures 3 1 Use only specifically designed centrifuge tubes and ensure they are properly sealed before centrifugation 3 2 Thin walled centrifuge tubes must be filled to within 5mm of top of tube to avoid tube collapsi
3. ions 9 1 Maintenance Record Sheet 11 Attachments 10 1 10 2 10 3 10 4 10 5 Beckman SW 55 Ti Rotor Instruction Manual Beckman Coulter Rotors and Tubes User s Manual Beckman Coulter Rotors and Tubes User s Manual Preface and Chapter 1 Beckman Coulter Rotors and Tubes User s Manual Chapter 7 Care and Maintenance Beckman Coulter Rotors and Tubes User s Manual Chapter 2 Tubes Bottles and Accessories and Chapter 3 Using Tubes Bottles and Accessories 11 Change History 11 1 11 2 Issue Number 1st Issue Date Issued 1 03 2005 Issue Number Date Issued Reason for Change THE UNIVERSITY OF NEWCASTLE DISCIPLINE OF MEDICAL BIOCHEMISTRY STANDARD OPERATING PROCEDURE PROCEDURE NO GEP 077 MOD 1st Issue Page 5 of 3 Procedure Type General Equipment Procedure Title Beckman SW 55 Ti Swinging Bucket Rotor ILLUSTRATION 9 1 MAINTENANCE RECORD SHEET ERN 432 Serial 8E 2070 DATE MAINTENANCE SIGNED
4. ng 3 3 In the event of a spill decontaminate rotor immediately follow spill procedures GDP 011 and disinfecting instructions 3 4 Wear appropriate personal protective equipment including lab gown and fully enclosed shoes 4 1 Check that the swinging buckets can swing up into position on the rotor spindle and properly fit the support framework 4 2 Label the racks on which the buckets are stored to indicate the rotor to which they belong 2 Calibration N A 3 Monitoring 3 1 Frequency Whenever in use 3 2 Method Listen carefully to the centrifuge when this rotor is in use to ensure its operating sounds are characteristic to the particular machine If there is any unfamiliar sound coming from this equipment when it is in operation it could possibly mean that the rotor is unbalanced and the centrifuge must be turned off immediately This is to avoid major damage to the centrifuge and possible safety hazards When in operation remain with the centrifuge and monitor until the maximum desired speed has been achieved THE UNIVERSITY OF NEWCASTLE DISCIPLINE OF MEDICAL BIOCHEMISTRY STANDARD OPERATING PROCEDURE PROCEDURE NO GEP 077 MOD 1st Issue Page 3 of 3 Procedure Type General Equipment Procedure Title Beckman SW 55 Ti Swinging Bucket Rotor 4 General Operation 10 2 Read the Safety Notice on page 3 of the Beckman Coulter Rotors and Tubes User s Manual Attachment 10 2 10 3 For General Operation Instr
5. uctions on use of the rotor refer to page 2 of the Beckman SW 55 Ti Rotor Instruction Manual Attachment 10 1 4 3 For Operation Instructions and selection criteria for use of tubes bottles and accessories refer to the Beckman Coulter Rotors and Tubes User s Manual Chapter 2 Tubes Bottles and Accessories and Chapter 3 Using Tubes Bottles and Accessories Attachment 10 4 5 Cleaning 1 Frequency 5 1 1 Cleaning must be carried out when required and immediately in the case of a spill 5 1 Method 5 2 1 Refer to page 7 6 of the Beckman Coulter Rotors and Tubes User s Manual Chapter 7 Care and Maintenance Attachment 10 3 5 3 Records Record all cleaning details on the Maintenance Record Sheet Illustration 9 1 6 Maintenance 6 1 Method Refer to the Beckman Coulter Rotors and Tubes User s Manual Chapter 7 Care and Maintenance Attachment 10 3 and to page 8 of the Beckman SW 55 Ti Rotor Instruction Manual Attachment 1 6 3 Records Record all Maintenance details on the Maintenance Record Sheet 7 Instruction Manual 7 1 Available Copy attached 8 Repairs 8 1 Check ERN for Service Company and warranty details 8 2 Contact the University Workshop for advice and repairs THE UNIVERSITY OF NEWCASTLE DISCIPLINE OF MEDICAL BIOCHEMISTRY STANDARD OPERATING PROCEDURE PROCEDURE NO GEP 077 MOD 1st Issue Page 4 of 3 Procedure Type General Equipment Procedure Title Beckman SW 55 Ti Swinging Bucket Rotor 9 Illustrat
Download Pdf Manuals
Related Search