User Manual
Contents
1. Scroll though the list with the cursor keys ft JL Enter desired function with D enter 8 1 Print protocols 1 graphic protocol 2 graphic protocols 3 print program 4 print all programs A B C quit D enter Scroll through the list with the cursor keys D Confirm the highlighted function with D enter 8 2 Switch beep on off There is an option for an audible signal whenever a program enters a pause and when the program is finished If this option is active the beep can be switched off by pressing any key of the keyboard with signal yes no A B C quit D enter In this window you can select whether there is a signal or whether there is not Using the cursor keys you can toggle between signal yes no Confirm your selection with D enter 26 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company 8 3 Select language The display of the TGradient can be switched from English to German Select language with cursor keys U it As you toggle between the two settings the language will change language German English A B C quit D enter To confirm your selection press D enter 8 4 RS232 Control The Tgradient can be programmed and controlled by Personal Computer The Tcontrol iT software is available with the order number 050 820 To acivate remote control the Tgradient has to be set to RS232 control mode For detailled information on controllin2. Om30s 2 A B header C pgm OK D enter Note To program special parameters you can scroll the display to the right Move the cursor to the right side of the display that is labeled with opt gt The following screen will appear Main funct aT c dt s Al s 1 5 00 2 5 00 3 5 00 4 5 00 A B header C pgm OK D enter Note To get back to the screen with the basic parameters move the cursor to the left side of the display labeled with Main funct Alternatively you can move the cursor to the extreme right Temp C time C A gradient C opt gt 1 65 0 Oh 5m Os 2 65 0 Oh Om30s 3 55 0 Oh 0m30s 4 35 0 Oh Om30s 2 A B header C pgm OK D enter Note In the main screen you will find an information that a special parameter has been modified This is indicated by a in the row labeled with opt gt 6 1 Program time increments To compensate for loss in enzyme activity each step within a loop can be extended from cycle to cycle Enter the desired time increment seconds in the row labeled with dt s This value will be added to the time value from cycle to cycle Instruction Manual TGradient Thermocycler 11 2009 19 Biometra An Analytik Jena Company Main funct dT c dt s Al e s 2 5 00 3 5 00 4 10 5 00 5 5 00 A B header C pgm OK D enter Note The time increment will have an impact on the total runtime depend
3. cursor with cursor keys lt gt through the name This is helpful if you have confirmed an wrong letter or if you want to change the name of an existing program Once the name is complete confirm name with C name OK Directory 3 program no 0 Name TEST 1 lid temp oC preheating on edit A B files C pgm OK D enter 4 5 Enter the lid temperature You can now enter a temperature for the heated lid Note Thanks to a new lid design significantly lower lid temperatures can be used for thermocycling The lower lid temperature leads to a higher temperature uniformity within the lid and thus to a more even temperature distribution in the tubes For instruments featuring the new lid design a maximum temperature of 99 0 C should be used 10 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company Directory 3 program no 0 Name TEST 1 lid temp 99 C preheating on edit A B files C pgm OK D enter 4 6 Select deselect pre heating of the lid You can choose whether the lid is pre heated before the program starts This is to avoid evaporation during the initial heating phase Note During the preheating of the lid the block is held constant at 25 C Directory 3 program no 0 Name TEST 1 lid temp 99 C preheating on edit A on off TB files C pgm OK D enter Note The default setting which is recommended for most applications is preheating
4. do not connect the cycler to a socket shared by a strong power consumer like a refrigerator or a centrifuge 11 5 Adaptation of protocols from other cyclers Since the TGradient is an extremely fast thermocycler it may be necessary to reduce the heating and cooling ramps to run protocols from other cyclers For the setting of the heating and cooling ramps see section 6 3 Alternatively the time settings may be extended Instruction Manual TGradient Thermocycler 11 2009 29 12 Appendix Biometra An Analytik Jena Company 12 1 Technical specifications TGradient 96 Sample capacity Thermal range 3 C 100 C Temperature accuracy 0 1 C Thermal homogeneity 0 3 C within 15 sec Range of Gradient 40 C Maximum heating speed 4 C sec Max cooling speed 3 C sec 96 x 0 2ml tubes 96 well microplates strips Heated lid High Precision Smart Lid for optimum lid pressure and excellent temperature uniformity Lid temperature range 30 0 99 0 C Line Voltage 115 Volt or 230 Volt Frequency 50 60 Hz Maximum power consumption 310 Watt Fuses see label on the back of the instrument Display LCD graphical display back lit Ports serial RS232 bi directional data transfer parallel Centronics printer Port for in tube sensor memory Total capacity 1500 steps in 10 directories with maximum 100 programs each Dimensions WxDxH 25 x 34 x 16 5 cm Weig
5. field labeled with you can select the target step for a backloop Temp C time C H gradient C opt gt 1 65 0 Oh 5m Os 2 65 0 Oh Om30s 3 55 0 Oh Om30s 4 35 0 Oh 0m30s 2 29 A B insert delete C pgm OK D enter Enter the number of backloops Note total cycler number n backloops 1 e g enter 29 for a total number of 30 cycles Instruction Manual TGradient Thermocycler 11 2009 13 4 11 Cool below ambient temperature Biometra An Analytik Jena Company Temp C time CE 2 65 0 Oh Om30s 3 55 0 Oh 0m30s 4 35 0 Oh Om30S 2 29 5 4 0 pause A B insert delete gradient C opt gt D enter C pgm OK To hold the temperature for an indefinite time enter 0 After pressing D enter pause is displayed Note The minimum temperature you can set is 3 C 4 12Save program Save program with C pgm OK The program data are stored in the permanent memory Directory 3 program no 0 Name TEST 1 number of steps 5 runtime 1h10m23s edit A B C D The runtime is calculated and displayed Note To view the current remaining runtime at any time of the run press A info in the main menu 14 Instruction Manual TGradient Thermocycler 11 2009 5 View edit program data Biometra An Analytik Jena Company You can check any existing program by scrolling through the editing mode with cursor keys gt Select the desired program
6. gradient C opt gt 1 65 0 2 3 4 A B insert delete C pgm OK D enter Enter a time for step one Note There is a convention on how time settings are entered in all BIOMETRA cyclers hours minutes seconds If you enter a number without point this value will be interpreted as seconds 300 gt 5 minutes To program minutes enter a after the number of minutes To enter hours enter after the number You can also enter any combination of hours minutes and seconds Example for 1 hour 30 minutes 20 seconds enter 1 30 20 The time values will be displayed in the following format Oh 00 m 00s Accept time setting with D enter or move to the next field with cursor key 4 8 Settemperature gradient Note Each temperature gradient is defined by two settings first the temperature in the middle of the block second the range of the gradient For example if you want to program a temperature gradient from 50 to 60 C enter 55 C as temperature an 10 C for the gradient Temp C time C gradient C opt gt 1 65 0 Oh 5m Os 2 65 0 Oh Om30s 3 55 0 Oh Om30s 10 C 43 A B insert delete C pgm OK D enter Note The maximum temperature gradient is 40 C Note You can program as many steps with temperature gradient as you want 12 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company Note You can even set an inverse temperature
7. gradient An inverse gradient has the higher temperature on the left side of the block and the lower temperature on the right side respectively Inverse gradient are set by adding the algebraic sign to the gradient value 4 9 View different temperatures of the gradient in the block To view the temperatures in the 12 different block rows press A Temp C time C gradient C opt gt wi 82 50 0 50 2 50 9 52 0 53 2 54 4 Se Why li DEn 86 8 S83 59 1 59 8 60 YC 3 55 0 Oh Om30s 10 C 4 Temperature difference left side right side In the first highlighted lane the temperatures from row 1 to 6 are displayed In the second highlighted lane the temperatures from row 7 to 12 are displayed Note While the TGradient is cycling you can view the current gradient temperatures The temperatures can be displayed online during each gradient step see section 7 4 As the program moves to the next step the last gradient temperatures are stored Thus you can check the temperatures of the last gradient in consecutive steps without gradient For further information on the current status of the cycler see section 7 4 10Set cycle number Note In general loops are defined by selecting the target for the back loop and the number of backloops Temp C time gradient C opt gt 1 65 0 Oh 5m Os 65 Oh Om30s 2 0 3 55 0 Oh Om30s 4 35 0 Oh Om30s 2 A B insert delete C pgm OK D enter In the
8. heating of feld 11 4 7 Enter temperature and time SettingS 2 c eecccceeeeeeeeeeeeeeeeeeee eee eeeeeeeeeeeeeeeeeeneneeees 11 4 8 Set temperature goradient AEN 12 4 9 View different temperatures of the gradient in the bock 13 4 10 Set cycle E 13 4 11 Cool below ambient Temperature 14 4 12 SAVE PrOQrann TEE 14 View edit e LC E DN 15 5 1 Delete insert program steps nu a a 15 5 2 COBY e E nalen 16 5 3 Delete Pfagran a aaa 17 Further programming Opllons 00 0 01 nun er ran 19 6 1 Program time increments aan 19 6 2 Program touch down ae eu 20 6 3 Adjust heating and Cooling rates ummrm444444444nnnnnnnnnnnnnnnnnnannnnnnnnnnnnnnnnannnnnen 20 El Be E E 21 7 1 Select and start program E 21 7 2 Display during operation E 22 7 3 View remaining run hl 23 7 4 View current gradient temperatures c cccccceeeeeeeseecneeeeeeeeeeeeesneeeneeeeeeseseneneseeeaeeees 23 7 5 Pauses SIOp FOO EE 24 SPEBIal TNC E 26 8 1 PIINTDIOBED Sana ee 26 8 2 SWitch beep ONION ann ae 26 8 3 Select language EE 27 8 4 RS232 e nenne saterencaasscontteansamecentaces 27 8 5 Gel 27 EE 28 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company 10 Exchange of modules u 0 0 a re 28 11 Trouble SHOT e 0000 RE an 29 Slow heating and COMING ana aeanaia E EAEE REEE EEE ENRE 29 Adaptation of protocols from other cyclers A 29 Slow heating and cooling une 29 Restart due to unrec
9. with a maximum range of 40 C can be set at any program step We have taken strong efforts to create a gradient as linear as possible At the same time it was very important to ensure homogenous temperature distribution within each row The temperature data for each row of the block can be retrieved through the help menu The new TGradient is an outstanding instrument In addition to the advanced temperature gradient it provides high speed and reliable accuracy for routine applications The silver block features very high heat conductivity and ensures fast ramping speeds and a fast temperature equilibration The new TGradient will soeed up your laboratory work because it saves time not only in optimizing new reactions but also in your routine work Instruction Manual TGradient Thermocycler 11 2009 1 Biometra An Analytik Jena Company 2 Before you start 2 1 Safety precautions Please read this manual carefully before starting operation of the TGradient Thermocycler General safety precautions for laboratory work must be observed when working with the TGradient thermocycler Be aware that samples are reaching high temperatures Do not touch or open hot tubes or microplates because hot liquid may quickly spill out Do not heat samples without having the lid locked securely Before opening of the lid release lid pressure see section 3 6 Do not place fingers between lid and housing when opening or closing the lid Do not touch th
10. 3 Start with the main screen Prog step temp C 25 2 C time lid 25 4 C A B start stop C programs D Press C programs to enter the edit mode subdirect subdirect Subdirect subdirect subdirect C quit D enter NO UI VS W Main direct W A You are now in the main directory The structure of the subdirectories is displayed 4 2 Select a program store Programs can be stored in the main directory or in subdirectories To create a program in the main directory press D enter To access a subdirectory move right with the cursor key gt and select a subdirectory with the cursor keys 4 The selected directory will be highlighted subdirect subdirect Subdirect subdirect subdirect C quit D enter NA UI VS W Main direct W A 8 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company 4 3 Enter name for subdirectory The process for naming a subdirectory and a program is the same For information about naming programs see section 4 4 Press D enter to enter the subdirectory directory 5 program no Name edit A list B C quit D enter Press A list to view a list of all existing files and empty memory in this directory Note If neither program name nor the word empty is displayed this place may be occupied by a program without name F
11. 6 1 2001 EN 61010 1 2001 EN 61010 2 010 1994 A1 1996 Dr J rgen Otte Quality Manager 34 Instruction Manual TGradient Thermocycler 11 2009 Formular 006 Ver 03 17 Subject Index Bern ee 26 Declaration of Conformity 34 Decontamination ccccccccecccseecssueeeeuaeees 33 Heated Jgd 7 Heated Lid E 11 LACS een 27 Mamtenance 28 Module Exchange engere 28 Program COPY nee 16 Grat errn a ENENEN 8 RTE 17 Graden 13 Heating and cooling rates 20 INCE MON serrare en 19 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company Insert Delete Step un 15 LOOD BEE 13 Name 9 Pause nenn 24 Remaining run time 23 EE 14 EE 21 COP ea 24 Protocol Ed LE 26 Safety precautions EE 2 SEITE E 6 Technical specifications sseeeeeeeeea 30 Trouble shosling a e 2 2 a 29 Vu 32 35
12. Biometra An Analytik Jena Company Weradient Thermocycler User Manual June 2009 Ord No Model 050 800 TGradient 48 050 801 TGradient 96 Please read these instructions carefully before using this apparatus Biometra GmbH Service Department Rudolf Wissell Str 30 Rudolf Wissell Strasse 14 16 D 37079 G ttingen D 37079 G ttingen Tel 49 551 50686 0 Tel 49 551 50881 10 12 Fax 49 551 50686 66 Fax 49 551 50881 11 email info biometra com email service biometra com Internet http www biometra com 9 Biometra An Analytik Jena Company DEV dee d e DE 1 Before YOU SUI ies nina neta eee nina aie esate eet 2 2 1 Safety ET CN 2 First steps with the TGradient nuussnnnsnnnnnnnnennnnnnnnennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnannnnnnnnnnnnnnn 3 3 1 The TGradient Thermocycler front View 3 3 2 The TGradient rear view EEN 4 3 3 The TGrad ent control panel eu ee nmnet 5 3 4 ge E 6 3 5 The TGradient display nennen 6 3 6 Handling of the adjustable heated Lid aanamee 6 3 7 Releasing wheel in case of blocked lid eccccececceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeees 7 PE Ee LC EE 8 4 1 Selecta CWE CIONY ae aR EE EE dE CERS aa a RE enai 8 4 2 Select a program Store wiasescccccccssscievss dacedceedscanctenastocarsnaessssmesaatacbecussscanibenedantadexendtniannede 8 4 3 Enter name for subdirectory ee ande nee 9 4 4 Enter a enn E 9 4 5 Enter the le te 10 4 6 Select deselect pre
13. all directories so called RAM check 3 5 The TGradient display The TGradient display provides information about the instrument and about the status of the active program prog step temp C 25 2 C time lid 25 4 C A B start stop C programs D The individual function of the special keys A B C and D are specified in the display and depend on the context 3 6 Handling of the adjustable heated Lid To achieve optimum pressure on the tubes the TGradient is equipped with an height adjustable heated lid Close the lid After the samples have been placed in the block close the lid Turn the wheel clockwise until you hear a clicking noise In this mode the pressure will not increase further even when you keep on turning the wheel Note The pressure of the lid has been optimized for a fully loaded block If only very few tubes are loaded to the block you should place dummy tubes in the four corner positions to avoid damage of tubes by excessive pressure Open the heated lid First Release pressure by turning the wheel counter clockwise As soon as there is no more resistance the pressure has been released Then now you can open the lid with the knob Important The lid should not be opened under pressure because this leads to damage of the locking mechanism 6 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company 3 7 Releasing wheel in case of blocked lid Note When
14. as described in section 4 2 Note During operation of the cycler the active program can be viewed but not modified If you want to change settings of the active program for further experiments you have to save a copy of this program to another memory For further information about copying programs see section 5 2 5 1 Delete insert program steps The programming spreadsheet Temp C time C gradient C opt gt 2 65 0 Oh Om30s 3 55 0 Oh Om30s 4 35 0 Oh 0m30s 2 29 54 A B insert delete C pgm OK D enter To delete or insert a program step press B insert delete insert delete step 2 65 0 Oh Om30s 3 55 0 Oh Om30s 4 35 0 Oh 0m30s 2 29 KS A B delete input C quit D enter Enter the number of the step that you want to insert delete Note Additional steps will be inserted before the selected step number The following steps will be shifted by one position insert delete step 3 2 65 0 Oh Om30s 3 55 0 Oh Om30s 4 35 0 Oh 0m30s 2 29 5 A B delete input C quit D enter Note If you have entered a wrong number the field can be cleared with B delete input Confirm the step number with D enter Instruction Manual TGradient Thermocycler 11 2009 15 Biometra An Analytik Jena Company insert delete step 3 2 65 0 Oh Om30s 3 55 0 Oh Om30s 4 35 0 Oh 0m30s 2 29 5 A insert B delete C quit D enter Press A insert to insert
15. e 1 of 30 temp C 49 9 55 0 60 0 gt 50 0 55 0 60 0 time Oh 0m23s gt 0h 5m 0s lid 98 9 C A info B start stop C programs D Press A info to call up the current remaining time calculation Additional information about active pgm Status Step 2 Cycle 3 of 30 Remaining time 2h 57m Stop or pause key B in main menu A gradient B C quit D The current remaining run time id displayed in line 4 7 4 View current gradient temperatures While the TGradient is cycling you can view the current gradient temperatures To retrieve detailed information about the temperatures in the different rows press A info in the main screen Instruction Manual TGradient Thermocycler 11 2009 23 Status Remaining time 2h 57m A gradient B Additional information about active pgm Stop or pause key B in main menu Biometra An Analytik Jena Company Step 2 Cycle 3 of 30 C quit D Then press A gradient remaining time 2h56m A gradient B Temperatures of current gradient 7126 74924249 6 5073 51 4 52 6 53 8 C T712 55 0 56 2 57 3 58459 1159 A9 C quit D Note The temperatures can be displayed online during each gradient step see section 7 4 As the program moves to the next step the temperature data of the last gradient are stored in the temporary memory Thus you can check the temperatures of the last gradient in consecutive steps Note If there has not been yet a gradie
16. e heated lid When only few samples are put in the block place additional tubes in the four corner positions This is to evenly distribute the lid pressure and prevents single tubes from excessive pressure Use of few tubes may result in damage of the tubes by excessive pressure Use only suited plastic ware in the TGradient thermocycler Tubes and plates must show good fit when placed in the thermoblock Only use tubes that are suited for high temperatures tight lids The TGradient Thermocyler contains no user serviceable parts Do not open the housing instrument Service and repair may only be carried out by the Biometra Service department or otherwise qualified technical personal Do not use the instrument when damages of the housing block cable or other parts are visible The TGradient Thermocycler must not be used with explosive flammable or volatile liquids Appropriate safety regulations must be observed when working with infectious or pathogenic material The thermoblock and the heated lid will reach high temperatures during operation Both thermoblock and heated lid can burn you Rapid heating of the thermoblock can cause liquids to boil explosively Always wear safety goggles during operation Close the lid before starting a program It is not necessary to apply oil into the opening of the block in order to improve the heat transfer between the block and the sample tubes gt PP If you still decide to
17. etra An Analytik Jena Company Main display Prog step temp C 25 2 C time lid 25 4 C A B start stop C programs D To select a program press B start stop start 3 subdirect 4 subdirect Main direct 5 Subdirect 6 subdirect 7 subdirect A C quit D enter Choose directory with cursor keys gt ft JL or enter main directory with D enter Note The path for the program that was modified most recently is automatically pre selected 3 subdirect 4 subdirect Main direct 5 Subdirect 6 subdirect 7 subdirect A C quit D enter Enter subdirectory with D enter Directory 5 program no Name Edit A list B C quit D enter Enter the number of the program you want to start Alternatively press A list to select a program from the list of all programs in this subdirectory Scroll through the list with cursor keys J Instruction Manual TGradient Thermocycler 11 2009 21 Ceman il empty empty empty empty list mb A HME B forward Biometra An Analytik Jena Company C quit D enter Confirm highlighted program with D enter The number and name of both subdirectory and th e selected program will be displayed 3 0 Directory program no start block Name TEST 1 preheating lid on signal on A list B C quit D start To start the program press D start 7 2 Dis
18. g Tgradient with PC please refer to the Tcontrol iT manual 8 5 Block type Display and calculation of gradient temperatures depends on the block version used A temperature gradient can be run with either 48 well and 96 well modules After changing block modules from one type to the other the block type must be set in the instruments software Go to special function 8 block type and set correct block version Instruction Manual TGradient Thermocycler 11 2009 27 Biometra An Analytik Jena Company 9 Maintenance The TGradient was built to operate for a long time without the need for periodical maintenance The housing may be cleaned from time to time with a smooth cotton cloth Do not use strong detergents or organic solvents for cleaning 10 Exchange of modules For exchange of modules you need two hexagonal keys number 3 and number 5 These keys are included in the shipment of the module Remove module Remove power plug Place instrument upside down on a soft surface Remove the 2 screws that close the shutter hexagonal key number 3 Remove the 4 screws that hold the block hexagonal key number 4 unplug connectors from the block control and power supply remove module OOOOOO Install module Follow description in reversed order 28 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company 11 Trouble shooting 11 1Slow heating and cooling The T1 Thermocycler is equipped w
19. ht 8 8 kg Storage conditions Operating conditions br 35 C 12 2 Ordering information TGradient Thermocycler Order No TGradient 48 for 48 x 0 5ml tubes 050 800 TGradient 96 for 96 x 0 2ml tubes strips or 96 well microplates 050 801 TGradient Modules TGradient 48 Module for 48 x 0 5ml tubes 050 810 TGradient 96 Module for 96 x 0 2ml tubes strips or 96 well microplates 050 811 TGradient 384 Module for 384 well microplates non gradient 050 812 For information about tubes strips microplates and other consumables please contact Biometra or your local Biometra distributor 30 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company 13 Instructions for return shipment In case of faults occurring with our TGradient Thermocycler please proceed as follows Return only defective devices For technical problems which are not definitively recognizable as device faults please contact the Technical Service Department at Biometra Tel 49 551 5068610 Fax 49 551 5068666 e mail info biometra de Important Cleanse all parts of the instrument of biologically dangerous chemical or radioactive contaminants If an instrument is contaminated Biometra will be forced to refuse to accept the device The sender of the repair order will be held liable for possible losses resulting from insufficient decontamination of the device Please prepare written c
20. ing on the numbers of cycles and the size of the increment A program with many cycles and large time increments will take a significantly longer time than a standard protocol 6 2 Program touch down For some applications it is useful to start with a higher temperature and to decrease the temperature from cycle to cycle This subsequent lowering of a temperature from cycle to cycle is called touch down To decrease a temperature from cycle to cycle enter a negative temperature increment in the row labeled with dT s Main funct aT C dt s a s 2 5 00 3 5 00 4 0 20 5 00 5 5 00 A B header C pgm OK D enter Note Be sure that the temperature decrease is set in a step that lies within a loop Otherwise there will be no iterative temperature decrease 6 3 Adjust heating and cooling rates Since the TGradient is a extreme fast thermocycler it may be necessary to reduce the heating and cooling ramp for some applications For example this is helpful if cycling protocols from other cyclers shall be used The heating or cooling ramp respectively can be set in the row labeled with A s Main funct dT c dt s Al e s 2 5 00 3 5 00 4 5 00 5 5 00 A B header C pgm OK D enter Note The default is 5 00 gel which means maximum ramping speed 20 Instruction Manual TGradient Thermocycler 11 2009 7 Start Program 7 1 Select and start program Biom
21. ith a strong ventilator for the cooling of the heat sink The inlet of this fan is located at the bottom side of the instrument Be sure that the inlet is not clogged by dust or other material e g a sheet of paper placed under the cycler can be attached to the inlet as the fan is in operation Dirt can be removed easily from the inlet with a conventional vacuum cleaner 11 2 Adaptation of protocols from other cyclers Since the T1 Thermocycler is an extremely fast thermocycler it may be necessary to reduce the heating and cooling ramps to run protocols from other cyclers For the setting of the heating and cooling ramps see section 6 3 Alternatively the time settings may be extended Note Protocolls that have been optimized with a TGradient thermocycler can be run without changes 11 3Slow heating and cooling The TGradient is equipped with a strong ventilator for the cooling of the heat sink The inlet of this fan is located at the bottom side of the instrument Be sure that the inlet is not clogged by dust or other material e g a sheet of paper placed under the cycler can be attached to the inlet as the fan is in operation Dirt can be removed easily from the inlet with a conventional vacuum cleaner 11 4Restart due to unrecognized power failure High voltage fluctuation can lead to an automatic restart of the thermocycler In this case the cycler restarts at the step where there power failure has occurred To avoid voltage fluctuation
22. ity G ttingen den 20 08 2004 im Sinne der EG Richtlinie Uber elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 73 23 EWG following the EC directive about electrical equipment for use within certain limits of voltage 73 23 EEC und and im Sinne der EG Richtlinie f r die elektromagnetische Vertr glichkeit 89 336 EWG following the EC directive about the electromagnetic compatibility 89 336 EEC Hiermit erkl ren wir da folgende Thermocycler und Thermocycler Module Herewith we declare that the following thermocyclers and thermocycler moduls Typen TGradient 48 TGradient 96 TGradient Modul 48 TGradient Modul 96 TGradient Modul 384 TGradient Modul in situ types TGradient 48 TGradient 96 TGradient module 48 TGradient module 96 TGradient module 384 TGradient module in situ Best Nr Order No 050 800 050 801 050 810 050 811 050 812 050 813 den grundlegenden Anforderungen der corresponds to the basic requirements of EG Niederspannungsrichtlinie 73 23 EWG und der EC low voltage directive 73 23 EEC and the EG Richtlinie ber die elektromagnetische Vertr glichkeit 89 336 EWG entsprechen EC directive about the electromagnetic compatibility 89 336 EEC Folgende harmonisierte Normen wurden angewandt The following harmonized standards have been used EN 55011 1998 A1 1999 A2 2000 EN 55022 1998 A1 2000 A2 2003 EN 61000 3 2 2000 EN 61000 3 3 1995 A1 2001 EN 61000
23. nstrument is operated as described in the manual Biometra will repair or replace each part which is returned and found to be defective This warranty does not apply to wear from normal use failure to follow operating instructions negligence or to parts altered or abused 32 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company 15 Equipment Decontamination Certificate To enable us to comply with German law i e 828 StriSchV 17 GefStoffV and 19 ChemG and to avoid exposure to hazardous materials during handling or repair will you please complete this form prior to the equipment leaving your laboratory COMPANY INSTITUTE ADDRESS TEL NO FAX NO E MAIL EQUIPMENT Model Serial No If on loan evaluation Start Date Finish Date Hazardous materials used with this equipment Has the equipment been cleaned and decontaminated YES NO delete Method of cleaning Decontamination NAME POSITION HEAD OF DIV DEP INSTITUTE COMPANY SIGNED DATE PLEASE RETURN THIS FORM TO BIOMETRA GmbH OR YOUR LOCAL BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING INSTRUMENTS WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER Instruction Manual TGradient Thermocycler 11 2009 33 Biometra An Analytik Jena Company 16 EU Konformitatserklarung EC Declaration of Conform
24. nt step in the protocol yet you will get the message No gradient data available To leave the window press C quit 7 5 Pause stop program Main display during operation Prog 0 TEST 1 Step 1 temp C 35 0 C time Oh Om18s lid 98 8 C A info B start stop dir 3 cycle 8 of 30 gt 35 0 gt 0h 0m30s C programs D To pause or stop the active program press B start stop Stop pause directory 3 program no O test 1 A B pause C quit D stop Select B pause to pause program D stop to stop program C quit to return to the program display When you select B pause the following screen is displayed 24 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company Prog 0 TEST 1 dir 3 Step 1 cycle 8 of 30 temp C 35 0 C gt 35 0 time pause lid 98 8 C A info B start stop C programs D The program pauses in the current state The word pause is displayed and alternates with the elapsed time You can proceed with the program with B start stop Instruction Manual TGradient Thermocycler 11 2009 25 Biometra An Analytik Jena Company 8 Special functions Main screen Prog step temp C 25 2 C time lid 25 4 C A B start stop C programs D To change special features of the TGradient press D print signal language standard mode test mode A back B forward C quit D enter Uu e Ww NIE
25. ognized power failUre cccccecseeeeeenceeeeeeeeeeseeeeeeeeeeeeeeneeneneees 29 Adaptation of protocols from other cyclers cessseeeecneeeeeeeeeeenseeeeeeeeeeenenseseneeeeees 29 12 APpeNdIK tee careers anise sede cantatas eaves ee 30 12 1 Technical specifications TGradient 96 u en 30 122 Ordering Mi rma tOM cr fa aa See eee atc area eee 30 13 Instructions for return shipment seeeEEEEEEEERKKEEREEERRREEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE 31 14 WAN PANY SENPRRRREREEREREERE SEEBEREEHRREREFEEEERFEFEFEREERREEEEEREFRFEREERRREEREREFERPEERBLEREREEEEEFEEEFREHEEREREEEREFERREPERERE 32 15 Equipment Decontamination Certificate ununussnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 33 16 EU Konformit tserkl rung EC Declaration of Conformity uusuuussnnnnannnennnennnn 34 17 EE TT E 35 Il Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company 1 Introduction The TGradient is the new high end thermocycler in the Biometra product line It was our goal to develop an instrument that provides fast optimization of reaction parameters as well as a high performance for daily routine analysis Both could be realized by a completely new block architecture and the latest Peltier technology To increase the specificity of a reaction the temperature is one of the crucial parameters With the new TGradient different temperatures can be tested within a single run A gradient
26. on After all pre settings have been made press D enter to open the programming spreadsheet 4 7 Enter temperature and time settings The programming spreadsheet Temp C time gradient C opt gt 1 2 ES 4 A B insert delete C pgm OK D enter Note In this soreadsheet you can enter all parameters for your cycling protocol Furthermore you can set special parameters like touch down ramping rates or time increments For further details on these special parameters see section 5 1 Note You can navigate in the spreadsheet without limitations with the four cursor keys eo TJ Note Each setting is confirmed with D enter The cursor moves automatically to the next field Alternatively you can confirm a value by moving forward with the cursor keys Note At any time you can call up the help function with A Note In an existing protocol program steps can be deleted and inserted For further information on deleting and inserting program steps see section 5 1 Instruction Manual TGradient Thermocycler 11 2009 11 Biometra An Analytik Jena Company Now enter temperature for the first step in the protocol Temp C time gradient C opt gt 1 65 0 2 3 4 A B insert delete C pgm OK D enter Confirm temperature with D enter or just move on with the cursor key If you move to the next row the setting will be stored in the temporary memory Temp C time
27. one step at the selected position Note Additional steps will be inserted before the selected step The following steps will be shifted by one position Press B delete to delete the selected step Note By deleting a step the following steps will be shifted by one position Note The insertion and deletion of steps may have an influence on existing cycles Be sure that all settings are updated to maintain a correct cycle 5 2 Copy program Select the program you want to copy as described in section 4 1 and 4 2 Directory 3 program no 0 Name TEST 1 lid temp 99 C preheating on A B files C pgm OK D enter Press B files Directory 3 program no 0 Name TEST 1 lid temp oC preheating on edit A copy B delete pgm C quit D enter To copy this program to another memory press A copy copy to 3 subdirect 4 subdirect Main direct 5 Subdirect 6 subdirect 7 subdirect A B C quit D enter Select the desired subdirectory with the cursor keys gt ft D The selected subdirectory will highlight 16 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company copy to 3 subdirect 4 subdirect Main direct 5 Subdirect 6 subdirect 7 subdirect A C quit D enter Confirm your selection with D enter copy from directory 3 program 0 to directory 6 program A list B C quit D enter Ch
28. onfirmation that the device is free from biologically dangerous and radioactive contaminants The declaration of decontamination see 15 has to be attached to the outside of the packaging Use the original packing material If not available please use a comparable robust box Be sure that the instrument fits tightly into the box When packaging protect the device in a way to avoid Label the outside of the box with CAUTION SENSITIVE ELECTRONIC INSTRUMENT e Please enclose a note which contains the following a Sender s name and address b Name of a contact person for further inquiries with telephone number c Description of the fault which also reveals during which procedures the fault occurred if possible Instruction Manual TGradient Thermocycler 11 2009 31 Biometra An Analytik Jena Company 14 Warranty This Biometra instrument has been carefully built inspected and quality controlled before dispatch Hereby Biometra warrants that this instrument conforms to the specifications given in this manual This warranty covers defects in materials or workmanship as described under the following conditions This warranty is valid for 24 month from date of shipment to the customer from Biometra This warranty will not be extended to a third party without a written agreement of Biometra This warranty covers only the instrument and all original accessories delivered with the instrument This warranty is valid only if the i
29. oose an empty memory from the list with A list copy from directory 3 program 0 to directory 6 program 6 enter OK target program contains 0 steps A list B C quit D enter Note Before you confirm with D enter the target memory is checked If there is no program in the target memory the message target program contains 0 steps is displayed If the memory already contains a program the number of steps is displayed Note If you copy a program to a memory that already contains a program the former program will be deleted Save a copy of the program in the new directory with D enter 5 3 Delete program Select program as described in section 4 1 and 4 2 TEST 1 Directory 3 program no 0 Name lid temp SG preheating on edit A copy B delete pgm quit D enter To delete this program press B delete pgm Instruction Manual TGradient Thermocycler 11 2009 17 Directory 3 program no 0 Name TEST 1 lid temp oC preheating on delete program 0 enter yes A copy B delete pgm C quit Biometra An Analytik Jena Company D enter Confirm your selection with D enter Note Once you have deleted a program it cannot be restored Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company 6 Further programming options Temp C time C gradient C opt gt 1 65 0 Oh 5m Os 2 65 0 Oh Om30s 3 55 0 Oh Om30s 4 35 0 Oh
30. or the programming of new protocols choose an empty memory Existing programs that are not needed any more can be deleted to provide new memory empty empty empty empty empty list B forward C quit D enter mb GAME Scroll through the list with cursor keys 4 and confirm memory with D enter 4 4 Enter a program name Each program is specified by a program number and a subdirectory number To make retrieval of a program easier you can enter a name for each program existing of letters numbers and symbols Directory 3 program no 0 Name A ABCDEFGHIJKLMNOPORST UVWXYZ C amp A ABC B files C name OK D enter To enter a name for your program press A ABC Instruction Manual TGradient Thermocycler 11 2009 9 Biometra An Analytik Jena Company Directory 3 program no 0 Name A ABCDEFGHIJKLMNOPORST UVWXYZ C Q amp A name B blank C quit D enter Choose a letter from the alphabet with cursor keys gt TU Confirm each letter with D enter Directory 3 program no 0 Name T A ABCDEFGHIJKLMNOPORST UVWXYZ C Q amp A name B blank C name OK D enter The selected letter will appear in the display and the cursor will move one step to the right Select the next letter and confirm with D enter Note What to do if a wrong letter has been accepted To alter the position of the cursor within the name press A name Now you can move the
31. play during operation During preheating of the lid the following screen is displayed Prog O TEST 1 dir 3 step 1 temp C 25 0 C gt 65 0 time lid preheating A info B start stop C programs D Note In the default setting preheating on the block is held constantly at 25 C The program starts as soon as the lid has reached the set temp erature During the preheating phase the display of the lid temperature alternates between the current lid temperature and preheating Prog 0 TEST 1 dir 3 step 1 temp C 25 0 C gt 65 0 time lid preheating A info B start stop C programs D As soon as the block has reached the programmed hold temperature the timer starts to count down 22 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company Prog 0 TEST 1 dir 3 Step 1 cycle 1 of 30 temp C 65 0 C gt 65 0 time Oh 2mi5s gt 0h 5m Os lid 98 9 C A info B start stop C programs D The elapsed time versus programmed hold time is displayed Prog O TEST 1 dir 3 Step 1 cycle 1 of 30 temp C 49 9 55 0 60 0 gt 50 0 55 0 60 0 time Oh 0m23s gt 0h 5m 0s lid 98 9 C A info B start stop C programs D As soon as the block enters a gradient step the three temperatures of the left middle and right sides of the block are displayed 7 3 View remaining run time Display during operation Prog O TEST 1 dir 3 Step 1 cycl
32. the lid is in the extreme up or down position it may happen that the wheel is uncoupled In this situation the clutch mechanism is active in both directions clicking noise in either direction To unlock wheel press metal pin with a ballpen and turn wheel carefully This pin overcomes the automatic clutch mechanism Thus care must be taken not to apply excessive pressure Lid arrested in upper position carefully turn wheel with pressed pin clockwise until you feel normal resistance no more clicking noise clutch is released Release pin and turn lid down until the clutch mechanism is activated clicking noise optimum pressure applied Lid arrested in down position carefully turn wheel with ce pressed pin counter clockwise until you feel normal resistance no more clicking noise clutch is released Release pin and turn wheel counter clockwise until pressure is completely released Open lid Important When the clutch mechanism is active optimum pressure is applied never use pin to further increase pressure This will lead to damage of tubes and instrument Instruction Manual TGradient Thermocycler 11 2009 7 Biometra An Analytik Jena Company 4 Creating a Program 4 1 Select a directory In the TGradient programs can be stored both in the main directory and in individual subdirectories For easy identification of the selected storage place the subdirectories can be named see section 4
33. use oil do not use silicon oil Mineral oil may be used Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company 3 First steps with the TGradient 3 1 The TGradient Thermocycler front View Wheel for the height Knob for release N adjustment of the lid of the heated lid Graphical display Power switch Instruction Manual TGradient Thermocycler 11 2009 3 Biometra An Analytik Jena Company 3 2 The TGradient rear view Parallel printer port RS 232 port Fuse holder A LL for 2 fuses U p d for specifications of fuses ower cor gt Sege see Ge label on the plug rege instrument s backside specifications of fuses 4 Instruction Manual TGradient Thermocycler 11 2009 Biometra An Analytik Jena Company 3 3 The TGradient control panel Graphical display Power Easy to clean keyboard backlit power on Power switch D D P Cursor keys Function keys For free navigation in the e f programming spreadsheet the corresponding functions are Use to scroll through lists specified in the screen Instruction Manual TGradient Thermocycler 11 2009 5 Biometra An Analytik Jena Company 3 4 Initial self test After switching on the TGradient the serial number of the instrument and the software version is displayed TGradient Serial No 1234567 Vers 4 00 op The TGradient checks all programs in
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