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Ettan IPGphor II Control Software

1. Hide Window Ctrl W Stops and exits the dialog Edit Cut Ctrl X Cuts selected text Copy Ctrl C Copies selected text Paste Ctrl V Pastes text Options Folders Ctrl F Allows the user to set the default folder for saved protocol files Help Help Ctrl F 1 Help on use of the software About Program ID information Using the Tab key on the PC allows the cursor to scroll through active or editable fields In the Protocol handling dialog Ctrl 4 will move the cursor down into the editable fields then Tab will allow scrolling through those fields Ctrl T will move the cursor out of the individual step lines Shift Tab allows the cursor to scroll backwards creating a new protocol 13 Creating a new protocol Protocol name To name a protocol highlight and delete the words New protocol and then enter the name you wish to identify the file by Protocol name New protocol The filename can contain an indefinite number of characters but only the first 16 characters will be displayed on the Ettan IPGphor II Isoelectric Focusing Unit display For example the protocol could be identified with the length of the IPG strip and the pH range that is being run Protocol name 24 cm 5 5 6 7 Range of parameters allowed for each step Rehydration time Rehydration time can be set from 1 minute to 99 hrs 59 minutes Temperature during rehydration Temperature during rehydration can be set between 15 C and 30 C This value c
2. New protocol a ni F Rehydration J O Hrs 5 0 Min Jj 20 C IEF R 50 p jstrip max di 20 c J j Number of Step Grad Voltage Hrsfvhrs Hrsfyhrs Min Step 1 j Gradient 1j 100 Jg Hs Jo as 2 4 LI Step 2 e Gradient J 0 J Hrs do 2 0 Step 3 t Gradient lo jus go do Step 4 Gradient So Hrs do Ao 4 A zi Step 5 tj Gradient 0 J Hs 10 Jo Step 6 21 Gradient So Hrs do to gt Step 7 Gradient 10 J Hs go 70 Step 8 e Gradient J 0 Jj Hrs Jo 0 Step 9 1 Gradient 0 J Hrs do 3 0 pll Ettan IPGphor Il Control Software e protocol handling dialog um 80 6500 47 p12 Protocol handling dialog menus and keyboard commands Drop down menus at the top left of the Protocol handling dialog can be accessed by clicking on the selected header to view the drop down menu options or can be accessed though the following keyboard commands menu bar description Protocol New Ctrl N Creates an empty protocol Open Ctrl 0 Opens a protocol from file Save Ctrl S Saves captured run data Print Ctrl P Prints the current protocol Load Ctrl L Loads the current protocol displayed to the Ettan IPGphor Il Isoelectric Focusing Unit Load All Loads retrieved protocols files 1 10 to the Ettan IPGphor Il Isoelectric Focusing Unit batch Retrieve All Retrieves all stored protocols files 1 10 from Ettan IPGphor II Isoelectric Focusing Unit batch
3. Protocols folder and click the Open button The stored protocol will now be displayed in the Protocol handling dialog If necessary make any adjustments to the values in the fields before proceeding to load the protocol Loading protocols To load the protocol displayed in the Protocol handling dialog first select a Protocol The Protocol 1 10 chosen here designates the protocol number on the Ettan IPGphor II Isoelectric Focusing Unit which will be overwritten by your loaded protocol Select the Protocol by using the up down buttons located to the left of the field Load current protocol to instrument Protocol Writing audi e To load a protocol either click the Load button select Load from the Protocol drop down menu or use the keyboard command Ctrl L The software will prompt the user that the protocol occupying that protocol number on the Ettan IPGphor II Isoelectric Focusing Unit itself will be overwritten If this is acceptable click i i xi 4re you sure Current protocol in instrument will be overwritten Cancel to continue creating a new protocol A yellow highlighting of the Writing icon indicates the protocol is being loaded The IPGphor application window is updated with the loaded protocol Load current protocol to instrument Protocol Writing al ug If the PC and Ettan IPGphor II Isoelectric Focusing Unit are in communi cation the protocol will be loaded i
4. Starts a Webserver Monitoring the Ettan IPGphor II Control Software application window from a Webserver is possible To activate the Webserver the Ettan IPGphor II Control Software program must be exited and restarted Stops the Webserver To deactivate the Webserver the Ettan IPGphor II Control Software program must be exited and restarted Help Help Ctrl F1 Help on use of the software About Program ID information Using the Tab key on the PC can scroll active or editable fields Shift Tab will move the cursor back Ettan IPGphor Il Control Software e IPGphor application window um 80 6500 47 p10 Setting Serial Interface Parameters The baud rate and the communication port used to communicate with the Ettan IPGphor II Isoelectric Focusing Unit can be set or checked by selecting Port under the Options drop down menu A baud rate of 1200 or 9600 can be used but must match the baud rate set on the unit A baud rate of 1200 is the default setting on the Ettan IPGphor II Isoelectric Focusing Unit Setting the default folder The default location to which log files will be saved is set to C MPGphor Logfiles This option can be changed by selecting Folder under the Options drop down menu Navigate to the folder or location you wish for saved data to be stored and click on the Select Cur Dir button Whenever files are saved from the IPGphor application window the new default folder will be displayed as the new l
5. communication between the PC and the Ettan IPGphor II Isoelectric Focusing Unit is broken the software will not recognize communication error but will no longer be collecting the data output from the instru ment Check the connections Once connec tion is reestablished the software will continue collecting data Closing the program is not necessary Note In the event of a power failure the soft ware cannot collect data from the Instrument and the data will not be recoverable um 80 6500 47 e p20 Old file logs from the last run are deleted prior to the instrument starting the run The software notifies the user of this action The instrument prompts the user to indicate how many IPG strips will be run The indicator icon for Run changes to green when the run has been started Run Pause The Ettan IPGphor II Isoelectric Focusing Unit display shows the run has started and the red HV light turns on While the run is starting the Instrument Log displays the instrument ID the firmware version the name of the protocol and the programmed steps of the protocol Data is collected at time 0 at the occurrence of a step change and at the desired time interval that has been set prior to the run The update interval can be adjusted during the run but previous data points cannot be deleted or added The graph will be updated as data points are collected and the x and y axis scale will change as the run progresses Note A
6. dialog will change slightly as the two fields hrs and minutes are not required to program this step Only the total VHrs for that step will be entered Number of Step Grad Volage Hrsjvhrs Hrsfwhrs Min Step 1 Gradient j100 1j vhs do creating a new protocol Editing numbers in the Voltage Hrs VHrs and minutes fields For fields that require a time or number to be entered use the up and down y buttons to the left of the field to change the value 1 digit at a time Alternatively you can highlight and use the keyboard to enter the desired value Saving protocols Once a protocol is created it can be saved for future reference To save a file either click on the Save button use the keyboard shortcut Ctrl S or select Save from the Protocol drop down menu The default location for the saved files is in the local C directory under CMPGphor Protocols The file will be specified by the protocol name and saved as a custom pattern Excel file xls If desired the user can change the default directory by selecting Folder under the Options drop down menu The default protocols folder will be shown If desired navigate to the location where files are to be stored and click on the Select Cur Dir button Printing Protocols A protocol may be printed from the Protocol handling dialog To print a protocol either use the keyboard command Ctrl P or select Print from the Protocol drop down
7. selecting Report or ID from the Run drop down menu Commu nication can also be checked by exiting and then restarting the Ettan IPGphor II Control Software program To exit the Ettan IPGphor II Control Software program select Exit from the Logfile drop down menu use the control keyboard commands Ctrl Q or press the X button located in the top right corner of the window The software will notify the user that any unsaved protocols that may have been created will be lost LT x Any unsaved log and protocol will be lost Continue exiting Click OK to continue to exit as the software must be exited and connec tion to the instrument established before proceeding 10 The Ettan IPGphor Il Control Software The Ettan IPGphor II Control Software has two main components an IPGphor application window and a Protocol handling dialog The IPGphor application window is used when controlling the Ettan IPGphor II Isoelectric Focusing Unit viewing the data as the IEF run progresses and printing or exporting collected data The second Protocol handling dialog is visible when Edit Protocol is selected The Protocol handling dialog allows for creating saving and loading protocols The IPGphor application window and Protocol handling dialog can be resized maximized or minimized 11 IPGphor application window Once the Ettan IPGphor II Software Control program is launched the appli cation window will appear In this main window
8. the Ettan IPGphor II is controlled and readings taken at selected intervals to be logged and graphed This window also allows the user to Open and view stored log files for previous runs Save print and export the collected data Open the Protocol handling dialog to create new protocols Start stop and pause the Ettan IPGphor Il Isoelectric Focusing Unit being controlled Report the status of the Ettan IPGphor Il Isoelectric Focusing Unit instantaneous report of run conditions when requested Capture the ID of the instrument serial number and firmware version Select the default log file directory Set the baud rate and the communication port Adjust the graph update interval Select the protocol to be run Enable the PC coupled to the Ettan IPGphor Il Isoelectric Focusing Unit to be monitored remotely through a web browser Fig 4 IPGphor application window e IPGphor application window LC io Logfile Protocol Run Options Help Edit Protocol Instrument log Serial e e Protocols Update interval Step markers 4 5 minutes di Current pA Hours Minutes Description of IPGphor application window Fig 4 Instrument Log The instrument log records data retrieved from the Ettan IPGphor II Isoelectric Focusing Unit once a run has started Edit protocol The edit protocol button takes the user to the Protocol handling dialog Here the user can create save load and store new
9. Portrait or Landscape and the number of copies 4 Printer setup E xl Printer selection j R amp D Lab HP LaserJet 8000 PCL Orientation di Portrait Copies Ap Printer and print orientation selections can be made either by clicking on the up down buttons y located to the left of the respective fields to scroll through the available choices or by clicking in the field to produce a pop up menu Select the number of copies by using the up down buttons located to the left of that field Exporting the log files to Excel To export the log files to Excel either select Export from the Logfile drop down menu or use the keyboard command Ctrl E When opening in Excel keep the default values Delimited Delimiters Tab Text Qualifier and Column data General Note Before exiting the Ettan IPGphor II Control Software make sure that all data collected has been saved printed or exported Closing the software will result in the loss of unsaved data exiting the software 16 Exiting the software To close the program either select Exit from the Logfile drop down menu use the keyboard command Ctrl Q or press the X button in the top right corner of the window When the software is closed a dialog box appears to confirm whether the program should be exited Any unsaved log and protocol will be lost Continue exiting 4 run is ongoing If you exit now you will loose data Continue exiting
10. Unit display If the correct protocol is not on the display use the Ettan IPGphor II Isoelectric Focusing Unit keypad or use the up down button located to the left of the field to select the desired protocol Adjusting the log file data capture time The time interval that data is captured from the Ettan IPGphor II Isoelec tric Focusing Unit can be adjusted by changing the Update interval on the IPGphor application window Use the up down button to adjust the inter val or click on the time field and select one of the time options with the pop up menu 1 minute You can also adjust the interval by selecting 2 minutes Graph update interval from the Options 3 minutes drop down menu Allowed intervals are 1 4 minutes 2 3 4 5 10 15 20 25 and 30 minutes For long runs it is recommended to set the 10 minutes interval for at least 10 minutes to avoid 15 minutes Instrument log data printouts that are many 20 minutes pages long di uo Once everything has been properly set the CL software can be used to control the Ettan IPGphor II Isoelectric Focusing Unit Starting a run To start the Ettan IPGphor II Isoelectric Focusing Unit either click the start button in the application window select Start from the Run drop down menu use the keyboard command Ctrl 1 or press Start on the Ettan IPGphor II Isoelectric Focusing Unit Run Pause e p19 Ettan IPGphor Il Control Software e running a protocol Note f
11. When OK is clicked a second dialog box requesting the user to stop the Ettan IPGphor Isoelectric Focusing Unit appears Click stop the unit and continue exiting x Stop ongoing run e p23 Ettan IPGphor Il Control Software e troubleshooting Note f communication between the PC and the Ettan IPGphor Il Isoelectric Focusing Unit is broken the software will not recognize a communication error but will no longer be collecting the data output from the instru ment Check the connections Once connec tion is reestablished the software will continue collecting data Closing the program is not necessary Note In the event of a power failure the soft ware cannot collect data from the Instrument and the data will not be recoverable um 80 6500 47 p24 17 Troubleshooting Printing seems to hang when using the control software Network printer was selected and the computer is not logged into the network Log in or if printing is no longer required press Cancel until the login dialog box disappears Cannot monitor through a web browser e Only one Ettan IPGphor Il Control Software copy can be monitored from a remote web browser From the server computer go to the Option menu and Enable or Disable the Webserver as necessary Restart the correct Ettan Il IPGphor Software copy that correlates to the Ettan IPGphor Il Isoelectric Focus ing Unit to be monitored Software copies are denoted by IPGphor x where x
12. an be controlled in 1 C increments pA The pA limit of the IEF run can be set from 1 to 200 pA strip 50 pA strip is the recommended value for IEF for all IPG strips This value can be changed from 1 200 pA in 1 pA increments Temperature of the run Temperature during IEF can be set between 15 C and 30 C This value can be controlled in 1 C increments Since strips are run in high concentrations of urea colder temperatures can lead to formation of urea crystals that block proper focusing Ideally IPG strips should be run at temperatures between 20 C and 25 C Step Grad The two options for the voltage change pattern are Step which sets the voltage at the selected value for the new step and then holds the voltage constant for the step duration and Gradient which increases the volt age linearly with respect to time from the value set in the previous step to the value set for the current step If the first step is set in gradient mode the voltage will increase starting from 0 Volts e p13 Ettan IPGphor Il Control Software creating a new protocol Note Depending on the pA limit of the IEF run the conductivity of the IPG strip rehydration solution and the sample Ettan IPGphor Il Isoelectric Focusing Unit may not operate at the set maximum voltage um 80 6500 47 e p14 Voltage The voltage of any step may be set from 1 8000 Volts A value of 0 in this field will cause the program to end rega
13. cusing Unit from a PC From the PC protocols can be created stored and loaded to the Ettan IPGphor II While isoelec TM tric focusing of the Immobiline DryStrip gels progresses the software records the isoelectric focusing IEF parameters over time When the IEF run is completed graph and log files are produced to document all steps of the IEF run The graph and log files can be printed or saved If desired the log file can be exported to programs such as Microsoft Excel Multiple Ettan IPGphor II units may be controlled from one PC by using a RS232 expansion module Expansion modules are available from National Instruments Each Ettan IPGphor II unit s individual IEF para meters are recorded independently See hardware requirements and installation instructions pl Ettan IPGphor Il Control Software um 80 6500 47 p2 2 Ettan IPGphor Il Control Software computer requirements and installation The Ettan IPGphor II Control Software is designed to work with PC s that have Windows 98 SE Second Edition or higher Windows NT or Windows 2000 3 Connecting the Ettan IPGphor Il to the PC Use the serial cable that comes with the Ettan IPGphor II Isoelectric Focusing Unit to connect the serial port located in back of the Ettan IPGphor II instrument to the appropriate COM port of the PC or RS232 expansion module 4 Ethernet connections and web browser for monitoring the Ettan IPGphor Il from a remot
14. d first visa260runtime exe The uninstall Ettan IPGphor Il Control Software program will start first if a previous version of the Ettan IPGphor II Control Software is already installed setup exe e Install IPGphor setup exe The default installation of the Ettan IPGphor II Control Software will be on the local C drive within the folder titled Amersham Biosciences An additional folder labeled IPGphor will be created on the C drive This is the default folder in which saved protocols and saved log files will be stored The program also loads a sample file of a protocol and a log file The sample files will be loaded in the folders C IPGphor Protocols and C lPGphor Logfiles respectively Ettan IPGphor Il Control Software e software installation Note The uninstaller application will not notify you when rebooting is necessary If you plan to re install NI VISA you should always reboot the machine after running the uninstaller Note To use the printing feature in Ettan IPGphor Il Control Software Internet Explorer 5 5 or later must be installed um 80 6500 47 e p4 Installing and uninstalling the Ettan IPGphor Il Control Software to control multiple Ettan IPGphor II Isoelectric Focusing Units Although up to 4 Ettan IPGphor II units can be controlled from a single PC 4 separate copies of the Control Software must be installed on that single PC To carry out the installation of a 2nd copy of the Control S
15. dditional data points can be collected while the unit is running by selecting the Report from the drop down menu However collecting multiple data points over a 1 minute interval may result in multiple y values on the same plot e running a protocol To pause the protocol either click the Pause button on the application window select Pause from the Run drop down menu use the key board command Ctrl 2 or press the Stop button on the Ettan IPGphor II Isoelectric Focusing Unit control panel The indicator icon for Pause changes to green while the Ettan IPGphor II Isoelectric Focusing Unit is in pause mode and the red HV light on the Ettan IPGphor II Isoelectric Focusing Unit turns off Run Pause To stop the protocol either click the sp button on the application window select Stop from the Run drop down menu use the keyboard command Ctrl 3 or press the Stop button twice on the Ettan IPGphor II Isoelectric Focusing Unit control panel Both the Run and Pause indicator icons darken and the red HV light on the Ettan IPGphor II Isoelectric Focusing Unit turns off Monitoring the run As the run progresses the Ettan IPGphor II Isoelectric Focusing Unit conditions are recorded in the Instrument log window and the data points graphed in real time The user may scroll through the log file values by using the scroll bar to the right of the log window Step markers The Step markers button functions to indicate where a ste
16. e default protocol folder with the default name Instrument protocols date and time A message displayed in the dialog box indicates protocols 1 10 on the Ettan IPGphor II Isoelectric Focusing Unit are being retrieved Once retrieved the user is prompted to save the protocols to disk with a Save As dialog box Files are saved within the default protocol folder with a default name Instrument protocols date time The files are stored as a custom pattern Excel text file Null protocols retrieved from the Ettan IPGphor II Isoelectric Focusing Unit i e those lacking any steps are not saved In order to be saved to disk a protocol must contain programmed steps If the Ettan IPGphor II Isoelectric Focusing Unit is not in proper commu nication with the computer an error message will appear i x Retrieving protocols Failed Check connection to instrument Check that the cable between the Ettan IPGphor II Isoelectric Focusing Unit and the PC is connected properly Communication can be confirmed by selecting Report or ID from the Run drop down menu Loading retrieved Ettan IPGphor II protocols Load all allows the user to reload stored protocols 1 10 to the Ettan IPGphor II Isoelectric Focusing Unit that have been retrieved as indicated in the section above When Load all is selected from the Protocol drop down menu Protocol handling dialog the software opens the Protocols folder on the C dri
17. e location One Ettan IPGphor II instrument can be monitored through a web browser This is only true within an internal network setting and cannot be viewed external to your computer network Internet Explorer 5 5 or later is required to monitor the Ettan IPGphor II instrument from another PC The web browser must be set for LAN settings and the IP address of the Ettan IPGphor II instrument must be accessible Contact your network administrator for assistance in establishing the connection 5 Hardware requirements If multiple Ettan IPGphor II Isoelectric Focusing Units are to be controlled by the Ettan IPGphor II Control Software the user must add a RS232 expansion box Such expansion modules are available from National Instruments Select the appropriate expansion module to fit with your computer connections e g USB to RS232 PCMCIA to RS232 Ethernet to RS232 Up to 4 Ettan IPGphor II units can be controlled from one PC software installation 6 Software installation Insert the Ettan IPGphor II Control Software CD The installation procedure will start up automatically Follow the onscreen instructions If the installer does not start up automatically select Run from the Windows Start menu and enter X Install bat where X designates the CD drive that contains the Control Software CD Installation performs the following actions O National Instruments Program NI VISA 2 6 Run time engine is installe
18. eagents Urea 500g 17 1319 01 CHAPS g 17 1314 01 Triton X 100 500 ml 17 1315 01 Dithiothreitol DTT 1g 17 1318 01 Bromophenol Blue Og 17 1329 01 Immobiline DryStrip Cover Fluid 000 ml 17 1335 01 Immobiline DryStrip gels 7 cm pH 4 7 2 7 6001 10 pH 3 10 2 17 6001 11 pH 3 10NL 2 7 6001 12 pH 6 11 2 7 6001 94 11 cm pH 4 7 2 8 1016 60 pH 3 10 2 8 1016 61 pH 6 11 2 17 6001 95 13 cm pH 4 7 2 7 6001 13 pH 3 10 2 7 6001 14 pH 3 10NL 2 7 6001 15 pH 6 11 2 7 6001 96 18 cm pH 4 7 2 7 1233 01 pH 3 10 2 7 1234 01 pH 3 10NL 2 7 1235 01 pH 3 5 4 5 2 7 6001 83 pH 4 0 5 0 2 7 6001 84 pH 4 5 5 5 2 17 6001 85 pH 5 0 6 0 2 7 6001 86 pH 5 5 6 7 12 7 6001 87 pH 6 9 2 7 6001 88 pH 6 11 2 17 6001 97 ordering information product quantity code number 24 cm pH 3 5 4 5 12 7 6002 38 pH 4 0 5 0 12 7 6002 39 pH 4 5 5 5 2 7 6002 40 pH 5 0 6 0 2 7 6002 41 pH 5 5 6 7 2 7 6002 42 pH 3 7 NL 2 7 6002 43 pH 3 10 2 7 6002 44 pH 3 10 NL 2 7 6002 45 pH 4 7 12 7 6002 46 pH 6 9 12 7 6002 47 Cleaning solution IPGphor Strip Holder 950 ml 80 6452 78 2 D Electrophoresis Principles and Methods 1 80 6429 60 Equilibration tube set for 24 cm IPG Strips 12 80 6467 79 IEF electrode strips 100 18 1004 40 e p27 Ettan Immobiline IPGphor and PlusOne are trademarks of Amersham Biosciences Limited Amersham and Amersham Biosciences are trademarks of Amersham plc MicroSoft Windows SE Excel Wind
19. fect Selecting ID from the Run drop down menu can capture the serial number of the Ettan IPGphor II Isoelectric Focusing Unit currently controlled by the software The ID of the instrument controlled will be displayed in the Instrument Log window This is helpful when multiple Ettan IPGphor II units are controlled from one PC Fig 5 The Protocol handling dialog protocol handling dialog 12 Protocol handling dialog To create a protocol click the Edit Protocol button in the top lefthand corner of the Ettan IPGphor II Control Software application window or select Open Protocol Window from the Protocol drop down menu The Protocol handling dialog will open to allow the user to create an IEF protocol Fig 5 The window will appear with default values of 0 for the rehydration time gradient steps 20 C for the rehydration and IEF set temperature 100 Volts 50 pA and 5 minutes run time are the default settings for step 1 The values for these parameters can be changed by either using the up n and down buttons to the left of each field or by inserting a new value for the field To change the time of the step it frequently is quicker to highlight and enter the desired value by using your keyboard than it is to use the up and down arrows to change the value I Protocol handling fd Protocol Edit Options Help Load current protocol to instrument Protocol Writing ou gi e Protocol name
20. is the number 1 4 assigned to the Ettan IPGphor II Isoelectric Focusing Units being monitored f a proxy server is used right click on Internet Explorer and select Internet Options under the Tools drop down menu Select the Connection tab Select LAN settings Mark bypass proxy server for local addresses or contact your network administrator The viewer will only work within a firewall Communication error e Check that the Ettan IPGphor II Isoelectric Focusing Unit is switched on Check that the serial cable is properly connected to the PC and the Ettan IPGphor II Isoelectric Focusing Unit Check to see that no other program is trying to access the communication port Close all programs that may be accessing the COM port Ordering information ordering information product quantity code number Ettan IPGphor Il Isoelectric Focusing Unit Basic unit Order strip holders separately 1 80 6505 03 Fuses 1 6 A 250V 5 x 20 mm 5 80 6108 69 Cable Serial 9 Pin F F with Null Modem Adapter 1 80 6506 36 Ettan IPGphor Il Control software 1 80 6506 55 Strip Holder Covers 7 cm 2 80 6455 44 11cm 2 80 6455 63 13 cm 2 80 6455 82 18 cm 2 80 6456 01 24 cm 2 80 6479 76 Strip Holders for use with Immobiline DryStrip gels 7 cm complete 6 80 6416 11 1 cm complete 6 80 6416 30 3 cm complete 6 80 6416 49 8 cm complete 6 80 6416 68 24 c
21. m complete 6 80 6469 88 7 cm complete 1 80 6416 87 1 cm complete 80 6417 06 3 cm complete 80 6417 25 8 cm complete 1 80 6417 44 24 cm complete 80 6470 07 Manifold Kit 80 6498 38 Immobiline DryStrip Reswelling Trays 7 18 cm 80 6371 84 7 24 cm 80 6465 32 IPG Buffer pH 4 7 ml 7 6000 86 pH 3 10 L ml 7 6000 87 pH 3 10 NL 1 ml 7 6000 88 pH 3 5 5 0 1 ml 7 6002 02 pH 4 5 5 5 1 ml 7 6002 04 pH 5 0 6 7 1 ml 7 6002 05 pH 5 5 6 7 1 ml 7 6002 06 pH 6 11 1 ml 7 6001 78 Rehydration Solutions DeStreak Rehydration Solution 1 ml 7 6003 19 DeStreak Reagent 5x3 ml 7 6003 18 e p25 Ettan IPGphor Il Control Software ordering information um 80 6500 47 p26 product quantity code number Ettan preparation kits and reagents 2 D Fractionation Kit 10 preps 80 6501 04 Albumin and 1gG Removal Kit 10 preps RPN6300 Protease Inhibitor lml 80 6501 23 Nuclease Mix 0 5 ml 80 6501 42 Thiourea 100 g RPN6301 lodoacetamide 25g RPN6302 Sample Grinding Kit 50 samples 80 6483 37 2 D Clean Up Kit 50 samples 80 6484 51 SDS PAGE Clean Up Kit 50 samples 80 6484 70 2 D Quant Kit 500 assays 80 6483 56 ini Dialysis Kit 1 kDa cut off 250 pl 80 6483 75 ini Dialysis Kit 1 kDa cut off 2 ml 80 6483 94 ini Dialysis Kit 8 kDa cut off 250 ul 80 6484 13 ini Dialysis Kit 8 kDa cut off 2 ml 80 6484 32 PlusOne r
22. menu A dialog box will appear Select the printer the print orientation Portrait or Landscape and the number of copies F Printer setup l ix Printer selection R amp D Lab HP LaserJet 8000 PCL Orientation e Portrait Copies J 1 Printer and print orientation selections can be made either by clicking on the up down buttons located to the left of the respective fields to scroll through the available choices or by clicking in the field to produce a pop up menu Select the number of copies by using the up down buttons located to the left of that field e p15 Ettan IPGphor Il Control Software creating a new protocol um 80 6500 47 e p16 Opening previously saved protocols Multiple numbers of protocols can be saved Once saved protocols can be recalled either by clicking the Open button by using the keyboard command Ctrl O or by selecting Open from the Protocol drop down menu Any steps programmed in the current protocol file will be deleted and the software prompt notifies the user of this action Any changes in current protocol will be deleted Continue Cancel If changes to the current protocol do not need to be saved click and continue To save any changes to the current protocol click cancel to abort opening the stored protocol Save the current protocol before attempting to reopen the stored protocol To continue opening a stored protocol select the saved file from the
23. menus at the top left of the Control Software application window can be accessed by clicking on the selected header to view the drop down menu options or can be accessed though the following keyboard commands menu bar description Logfile Open Ctrl 0 Opens a log file from a previously saved text file Save Ctrl S Saves captured run data as a text file Print Ctrl P Prints the current log data shown in the Instrument Print Graph Ctrl G Print All Ctrl A Export Ctrl E Exit Ctrl Q Protocol Log window Prints the graph currently displayed Prints the Instrument Log data and the graph currently displayed Exports log data to spreadsheet Stops and exits the program Open Protocol Ctrl W Window Run IPGphor control functions Opens the Protocol handling dialog Start Ctrl 1 Starts a protocol indicate no of IPG strips to be run Stop Ctrl 3 Stops a protocol Pause Ctrl 2 Pauses a protocol Report Retrieves current data from the Ettan IPGphor Il instrument and displays it in the Instrument Log window ID Retrieves the Ettan IPGphor Il instrument serial number amp firmware revision Options Folders Ctrl F Allows the user to set the default folders for log files Port Set serial port options and Baud rate Graph Update Interval Webserver Enable Webserver Disable Com port number 1200 or 9600 Baud Selectable update rates at 1 2 3 4 5 10 15 20 25 or 30 minute intervals
24. nto the designated protocol number on the instrument The display on the Ettan IPGphor II will indicate the new file name and save the protocol into the protocol number that was designated when loading Communication problem Load protocol probably failed Please check connection and if instrument is switched on If the PC and Ettan IPGphor II Isoelectric Focusing Unit are not in proper communication an error message will appear Check that the cable between the Ettan IPGphor II Isoelectric Focusing Unit and the PC is connected properly Communication can be confirmed by selecting Report or ID from the Run drop down menu Retrieving all preprogrammed IPGphor protocols Files that exist within the Ettan IPGphor II Isoelectric Focusing Units memory can be captured to the PC This allows the archiving of proto cols that are no longer frequently used and frees up needed file space on the Ettan IPGphor II Isoelectric Focusing Unit Under the Protocol drop down menu select Retrieve all A communica tion window will appear to confirm the action to continue to retrieve the files Retrieving all protocols will take up to 40 seconds Continue NN The software will indicate it is reading protocols 1 10 by displaying a message in the top portion of the dialog Reading protocols e p17 Ettan IPGphor Il Control Software creating a new protocol um 80 6500 47 p18 Files are saved under th
25. o Go to the client computer Open the web browser and enter the address http xxx IPGphor htm where xxx is the TCP IP address of the server computer obtained in step 1 above and IPGphor is the file name that is found on the window title of the Ettan IPGphor II Control Software that is webserver enabled possible alternatives are IPGphor IPGphor1 IPGphor2 IPGphor3 and IPGphor4 e Ettan IPGphor Il Control Software Ettan IPGphor Il Control Software 7 Overview The Ettan IPGphor II Control Software can be used to control up to 4 Ettan IPGphor II units at one time each running a different set of run parameters In addition to creating saving and editing protocols the soft ware monitors voltage current and VHrs volt hours of the run and graphs them as the run proceeds over time The x and y scale values used to graph the Log Data change over time and are dependent on the maxi mum values reached Contextual help will appear as the mouse is moved across active buttons to guide the user in navigating and using the soft ware More information for several of these functions can be obtained by right clicking over the active button hot spot 8 Opening the Ettan IPGphor Il Control Software From the Windows Start menu select Programs Select the IPGphor program from the menu When controlling multiple Ettan IPGphor II units off one PC each individual Ettan IPGphor II Control Software program 2 3 or 4 must also be
26. ocation for files to be saved Webserver enable The Ettan IPGphor II Control Software can be used to monitor an Ettan IPGphor II Isoelectric Focusing Unit from another PC In order to moni tor the Ettan IPGphor II Isoelectric Focusing Unit the Webserver must be enabled and the Ettan IPGphor Control Software program closed and restarted Internet Explorer 5 5 or later is required On the server computer select Webserver Enable from the Options drop down menu Record the http TCP IP address as indicated on the pop up window Close the Ettan IPGphor II Control Software The Ettan IPGphor II Control Software program should now be restarted In the remote PC open the web browser For Internet Explorer select Internet Options under the Tools menu bar and select the Connections tab Select LAN settings Mark bypass proxy server for local addresses Enter the web address http xxx IPGphor htm where xxx is the TCP IP address of the server computer and IPGphor is the Ettan IPGphor II software installed file IPGphor IPGphor 1 IPGphor 2 IPGphor 3 or IPGphor 4 If established properly the Ettan IPGphor II Control Software application window should be displayed and the Ettan IPGphor II Isoelectric Focus ing Unit run can be monitored from a remote location Webserver disable Selecting Webserver Disable from the Options drop down menu disables Webserver The program needs to be closed and restarted for the change to take ef
27. oft ware select the CD drive containing the Control Software CD then open the folder InstallIPGphor2 and double click on InstallIPGphor2 bat If a 3rd and or 4th copy needs to be installed repeat the procedure by opening the folder InstallIPGphor 3 or InstallIPGphor 4 and double clicking on InstallIPGphor3 bat or InstallIPGphor4 bat The first time the program is started the default port will be COM 1 Select a different COM path 2 through 8 for each Ettan IPGphor II Isoelectric Focusing Unit depending on the COM ports installed on the computer Contact your network administrator for more information Installing and uninstalling NI VISA 2 6 Before you install NI VISA 2 6 we strongly recommend that you unin stall any versions of VISA prior to NI VISA 2 0 1 NI VISA 2 6 should upgrade properly over NI VISA versions 2 0 1 through 2 5 2 After uninstalling NI VISA some DLLs and executables may remain in the NI VISA directories To complete the uninstall process you must restart your machine Installing and uninstalling Ettan IPGphor Il monitor embedded feature on the WEB client In this feature the monitoring of one Ettan IPGphor II unit is possible O From the server computer go to the Option menu and select Webserver enable Write down the TCP IP address from the pop up message You will need this for step 2 below Exit the Ettan IPGphor II Control Software and restart for the change to take effect
28. ows NT and nternet Explorer are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other counties Triton is a trademark of Union Chemicals and Plastics Co Amersham Biosciences Corp 2003 All rights reserved All goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Biosciences group that supplies them A copy of these terms and conditions is available on request Printed in the USA www amershambiosciences com Amersham Biosciences UK Limited Amersham Place Little Chalfont Buckinghamshire England HP7 9NA Amersham Biosciences AB SE 751 84 Uppsala Sweden Amersham Biosciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 USA Amersham Biosciences Europe GmbH Munzinger Strasse 9 D 79111 Freiburg Germany Amersham e Biosciences
29. p transition has occurred e g changing from step 1 to step 2 etc To mark where the steps have occurred click the button so that the green indicator is on Step markers To return to uniform vertical gridline marks along the x axis click the button so that the indicator is darkened Step markers Gi p21 Ettan IPGphor Il Control Software e capturing run data The size of the graph printed is dependent on the size the graph is viewed onscreen To enlarge the graph either expand or maximize the Logfile window um 80 6500 47 p22 15 Capturing run data Storing log files The log of a file can be stored and recalled for future reference To store a log file select Save from the Logfile drop down menu The default file name is the date and time e g 05 02 2003 01 59 43 PM but this can be edited The default location for the saved file is C PGphor Logfiles These files can be reloaded into the software at any time to view data and graphs Printing log files and graphs To print the log file only select Print from the Logfile drop down menu or use the keyboard command Ctrl P To print the graph only select Print Graph from the Logfile drop down menu or use the keyboard command Ctrl G To print the log file and the graph select Print All from the Logfile drop down menu or use the keyboard command Ctrl A A dialog box will appear Select the printer the print orientation
30. protocols as well as access previously saved protocols Serial Indicates the serial number of the Ettan IPGphor II Isoelectric Focusing Unit that is controlled by the software Protocol Indicates the Protocol selected to start from the Control Software Step markers When activated marks the locations of a step change by placing the vertical gridlines on the graph at the time of a step change When inactive uniformly spaced vertical gridlines are displayed on the graph Ettan IPGphor Il Control Software e IPGphor application window um 80 6500 47 p8 Update interval Changes the time interval at which the software captures data from the Ettan IPGphor II Isoelectric Focusing Unit Start Pause and Stop Controls the Ettan IPGphor II Isoelectric Focusing Unit Run and Pause indicators Indicates the status of the Ettan IPGphor II Isoelectric Focusing Unit as it is monitored by the Control Software Graph The Ettan IPGphor II Control Software graphs the data Voltage Volt hours and Current as it is retrieved from the Ettan IPGphor II Isoelectric Focusing Unit The x and y scale values change to the maximum value as the run progresses The default colors for the data markers and trendlines are black for the Voltage red for the accumulated VHrs and light blue for the Current in pA e IPGphor application window Ettan IPGphor Il Control Software application window menus and keyboard commands Drop down
31. rdless of what values are set for any subsequent steps Hrs VHrs Each step of the protocol can be programmed to run for a specified number of hours in hours and minutes or for a specific number of VHrs Volt hours is the product of the voltage multiplied by the number of hours for each step Each VHrs step may be set from 1 99 999 VHrs If more than 100 000 VHrs is desired it will have to be split between two steps The maximum value for Hours and minutes is 99 hours and 59 minutes At least 1 minute or 1 VHr must be programmed for a step or the program will end at that step regardless of what values are set for any subsequent steps Editing fields Editing modes for Hrs VHrs and Step Gradient The Hrs VHrs and Step Gradient fields each have two options which can A be selected by using the up down arrows located to the left of each field Alternatively clicking on one of these fields produces a pop up menu allowing you to select one of the options For example To change the voltage transition mode from gradient to step n hold mode click in the Step Grad field you wish to change A pop up menu will appear with the two modes allowed Select a mode by holding down the mouse and highlighting the mode desired Unclick the mouse and the selected mode will appear in the window Number of Step Grad Volage HrsjVvhrs Hrs vhrs Min Step 1 2 v Gradient 100 Hrs lo js Stan mL lin n Live lin iin In the VHrs mode the
32. started from the Programs menu 9 Communication between the Ettan IPGphor Il and PC When the Ettan IPGphor II Control Software is initiated the software confirms it is in communication with the Ettan IPGphor II Isoelectric Focusing Unit If successful a window will pop up indicating communi cation has been established PS x Communication with instrument is established If the software does not recognize connection between it and the Ettan IPGphor II Isoelectric Focusing Units then an error dialog will appear LT x Communication problem Please check connection and settings po Ettan IPGphor Il Control Software Note Check to see that no other program is trying to access the communication port Close all programs that may be accessing the COM port Note f communication between the PC and the Ettan IPGphor II Isoelectric Focusing Unit is broken the software will not recognize a communication error but will no longer be collecting the data output from the instru ment Check the connections Once connec tion is reestablished the software will continue collecting data Closing the program is not necessary Note In the event of a power failure the soft ware cannot collect data from the Instrument and the data will not be recoverable um 80 6500 47 p6 Check that the cable between the Ettan IPGphor II Isoelectric Focusing Unit and the PC is connected properly Communication can be confirmed by
33. user manual 2 D electrophoresis and first dimension IEF Ettan IPGphor II Control Software Amersham um 80 6500 47 Rev A0 06 03 e e B OSC l e n ces Page finder Al IntroduCHOD 2 335 arie ii 1 2 Ettan IPGphor Il Control Software 0 2 computer requirements and installation 3 Connecting the Ettan IPGphor Il to the PC 2 4 Ethernet connections and web browser 2 for monitoring the Ettan IPGphor Il from a remote location Hardware requirements LL 2 Software installl atiohi sia ie ae a 3 Ettan IPGphor II Control Software TOVERI EW ia pala des Dewy Hoe aa i ded deed 6 8 Opening the Ettan IPGphor Il RR RR m RR A ee iS 9 Communication between the Ettan IPGphor Il and PC 6 10 The Ettan IPGphor Il Control Software 6 11 IPGphor application window LL 6 12 Protocol handling dialog wins uo sce negem ia en a a 8 11 I3 Creating anew protocol sss ss nk man nk Ka Lach AU bene hok d 13 LA RUNNING a protocol 4 za sank pax Re E anke ike eae Ga HE Erica s 19 15 Capturing r m data amp eed VIS Suy EZ FPE 22 16 Exiting the Software iussa ek kk ATALA E iE ERU Ich RR RC ALA 23 17e TOUBIESMOOEING iano ca ia mtt dee ede tea edt aos DR a Es 24 Ettan IPGphor Il Control Software um 80 6500 47 pii introduction 1 Introduction to Ettan IPGphor Il Control Software The Ettan IPGphor II Control Software is used to control the Ettan IPGphor II Isoelectric Fo
34. ve Select the instrument protocol set you wish to load A message in the top portion of the window indicates the protocols 1 10 are being loaded and displays the number of lines written to the Ettan IPGphor II Isoelectric Focusing Unit Lines written Closing the Protocol handling window Once a protocol has been loaded click on the Hide Window button to close the Protocol handling dialog Note Pressing Start on the Ettan IPGphor Il Isoelectric Focusing Unit causes the software to automatically save the data displayed in the application window The file is saved in the default directory with the name of the protocol the date and the time the file was saved For example 18 cm 6 11 05 15 2003 03 54 59 PM txt To avoid autosaving the data start the Ettan IPGphor Il Isoelectric Focusing Unit using the software The software prompts the user to select whether the displayed data should be deleted e running a protocol 14 Running a protocol Once a file has been created and loaded to the Ettan IPGphor II Isoelectric Focusing Unit the instrument can be controlled by either using the software or by manually starting the instrument using the IPGphor keypad Prior to starting the software confirm the protocol to be run The soft ware automatically prepares to run the last protocol loaded written to the instrument Select the correct protocol to run by confirming the protocol on the Ettan IPGphor II Isoelectric Focusing

Ettan IPGphor II Control Software

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