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1. AmpF2STR COfiler pplied PCR Amplification Kit AS ci Biosystems Product P N 4305246 For Research Forensic or Paternity Use Only Insert P N 4305253 REV G Not for use in diagnostic procedures Printed in USA List of Kit Components Sufficient for 100 Tests Reagent Volume Description AmpF STR PCR Reaction Mix 1 1 mLftube 2 tubes of PCR Reaction Mix containing MgClz dATP dGTP dCTP and dTTP Bovine Serum Albumin and 0 05 sodium azide in buffer and salt Store at 2 C to 8 C AmpF STR COfiler 1 1mL 1 tube containing locus specific 5 FAM J OE and NED dye labeled and unlabeled primers in buffer that amplify the STR loci D3S1358 D16S539 THO1 TPOX CSF1PO D7S820 and the gender marker Amelogenin Store at 2 C to 8 C protected from light Primer Set AmpliTaq Gold DNA Polymerase 0 05 mL tube 2 tubes of enzyme with an activity of 5 U uL Store at 15 C to 25 C AmpF STR Control DNA 9947A 0 3 mL 1 tube containing 0 10 ng mL human cell line DNA in 0 05 sodium azide and buffer The genotype of this female DNA is D3S1358 14 15 D16S539 11 12 THO1 8 9 3 TPOX 8 8 CSF1PO 10 12 D7S820 10 11 Store at 2 C to 8 C AmpF STR COfiler Allelic Ladder 50 uL 1 tube of AmpF STR COfiler Allelic Ladder containing the following amplified alleles D3S 1358 alleles 12 to 19 5 FAM dye D16S539 alleles 5 8 15 5 FAM Amelogenin alleles X Y OE dye THO1 alleles 5 to 10 including2. F STR Profiler Plus Kit User s Manual P N 4303501 are followed The user s manual is purchased separately see Required Materials Required Materials Not Included Material P N Description AmpF STR COfiler 4305469 The AmpF STR COfiler User Bulletin contains the supporting User Bulletin documentation for the AmpF STR COfiler Kit Included is information on the kit characteristics a summary of results and interpretation issues and forensic validation and population genetics data AmpF STR Profiler Plus PCR 4303501 The AmpF STR Profiler Plus PCR Amplification Kit User s Manual Amplification Kit User s Manual contains all the necessary typing protocols for use with the AmpF STR COfiler PCR Amplification Kit Included are protocols for amplification in the Applied Biosystems DNA Thermal Cycler 480 and GeneAmp PCR Systems 9600 and 2400 and detection on the ABI PRISM 377 DNA Sequencer and ABI PRISM 310 Genetic Analyzer To obtain optimal results itis critical that the procedures detailed in the user s manual are followed GeneScan 500 ROX Internal Lane 401734 2 tubes each containing 200 uL of size standard Loading buffer is Size Standard included as a separate tube Performance Characteristics The AmpF STR COfiler PCR Amplification Kits are developed and manufactured by Applied Biosystems Each lot of AmpF STR COfiler Kits are carefully tested by Applied Biosystems to e
3. n or by estoppel Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA This product is covered by U S Patent No 5 364 759 owned by Baylor College of Medicine and is sold under license from Baylor College of Medicine Not for re sale Copyright 2006 Applied Biosystems All rights reserved Applied Biosystems AB Design ABI PRISM AmpF STR COfiler GeneAmp and Profiler Plus are registered trademarks and Applera FAM GeneScan J OE NED and ROX are trademarks of Applera Corporation or its subsidiaries in the U S and or certain other countries Product Insert AmpliTaq Gold is a registered trademark of Roche Molecular Systems Inc August 2006 All other trademarks are the sole property of their respective owners
4. nsure that the kits perform according to specifications and are free from interfering contaminants The user of the AmpF STR COfiler Kits will be able to amplify and type two nanograms of AmpF STR Control DNA 9947A when using the reagents provided in the kits and following the protocols described in the AmpF STR Profiler Plus User s Manual on an ABI PRISM instrument The recommended range of input sample DNA is approximately 1 0 2 5 ng At Applied Biosystems the kit components have been used successfully to type samples containing less than one nanogram of human DNA For Research Forensic or Paternity Use Only Not for use in diagnostic procedures NOTICE TO PURCHASER LIMITED LICENSE Use of the AmpF STR COfiler PCR Amplification kit is covered by one or more of the following US patents and corresponding patent claims outside the US 5 079 352 5 789 224 5 618 711 6 127 155 5 677 152 claims 1 23 only and 5 773 258 claims 1 and 6 only and claims outside the US corresponding to US Patent No 4 889 818 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product solely in forensic and paternity testing including reporting results of purchaser s activities for a fee or other commercial consideration and also for the purchaser s own internal research No right under any other patent claim is conveyed expressly by implicatio
5. the 9 3 allele J OE dye TPOX alleles 6 to 13 J OE CSF1PO alleles 6 to 15 OE dye D7S820 alleles 6 to 15 NED dye The allelic ladder contains PCR product do NOT amplify To avoid contamination store the allelic ladder separate from the other kit contents and unamplified DNA Store at 2 C to 8 C protected from light CHEMICAL HAZARD AmpliTaq Gold DNA Polymerase may cause eye and skin irritation Exposure may cause discomfort if swallowed or inhaled Read the MSDS and follow the handling instructions Wear appropriate protective eyewear clothing and gloves Caution The AmpliTaq Gold DNA Polymerase should be removed from the kit upon receipt and stored at 15 C to 25 C Caution Reagents used for amplification must be isolated from any source of contaminating DNA especially from_previously amplified AmpF STR PCR products and the AmpF STR Allelic Ladders Caution The AmpF STR COfiler Primer Set and the Allelic Ladder contain primers that are labeled with a light sensitive dye These reagents should be stored protected from light and not left exposed on the laboratory benchtop Caution Sodium azide is used as a preservative in some reagents It can react with lead and copper plumbing to form explosive metal azides On disposal of the reagent flush with large volume of water to prevent azide build up To obtain the optimized amplification and typing results it is critical that the protocols in the Amp
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