Manual euth unit ENG.indd
Contents
1. USER s Manual for the UNO Euthanasia UNIT Before operating the UNO Euthanasia Unit please read this manual On the trolley of the UNO Euthanasia Unit 3 gasbottles can be placed and secured safely One should at least place 1 gasbottle with Carbogen mixture 95 O 5 CO and 1 gasbottle CO on the trolley and connect these A third gasbottle could be a spare canister with CO or a different kind of gas for special purposes T The hoses on the UNO Euthanasia Unit are to be connected to the gasbottle by a so called 2 stage pressure reducing valve With this pressure reducing valve you can reduce the outlet pressure of the bottle to lt O 5bar Plug the UNO Euthanasie Unit into a wall socket with earth 220V 50Hz Switch on the UNO Euthanasie Unit see figure A Figure A Programs 1 min Carbogen 4 min CO Signalizing gasses 1 min Carbogen 1 min CO gt CO C arbogen Manual control manual Euth unit 06 2004 L 5 Adjust the pressure of the gas supply per gas this should be done by following the below mentioned procedure Sa After the UNO Euthanasia Unit has been switch on by pushing the red button Figure A the green button marked Carbogen Manual control is pushed This will light up which means dat de valve for this gas is open Now open the 2 stage pressure reducing valve on the gasbottle with Carbogen2. and reduce the pressure to 0 5 1 0 bar depending on the size of the container Now again push on the green button Carbogen at the manual control The green light will extinguish showing that the valve for this gas is closed When the valve for the Carbogen closes de pressure on the 2 way pressure reducing valve will go up because there is no longer a gasflow present NOTE Wenn the valve opens you should hear a click of the magnet in this valve In case that the light bulb in the green button is defect you can still hear the valve opening up 5b The procedure described under 5a is now to be repeated for the CO2 gas For this gas you should also adjust the 2 way pressure reducing valve to 0 5 1 0 bar depending on the size of the container 5c After you have completed the procedures described in 5a and 5b the UNO Euthanasia Unit is ready for use 6 Programs Program 1 1 min Carbogen 4 min CO This program is to be used to euthanise mice and rats in accordance with the procedure described below Procedure The UNO Euthanasia Unit contains a program that uses 2 different gasses meant to reduce the animal s stress to a minimum during the euthanasia proces of mice or rats During the euthanasia the following is important The animal can stay in their cage because the UNO Euthanasia Unit includes a transparant polycarbonate container in which the complete rat or mousecage can be
3. lves on the gasbottles Contact UNO gt UNO Roestvaststaal BV PO Box 15 6900AA ZEVENAAR The Netherlands Phone 31 316 524451 Fax 31 316523785 Email info unobv com manual Euth unit 06 2004 LI B
4. placed First Carbogen gas is introduced at the bottom of the cage by means of a PLC controlled valve Carbogen is a gas mixture of 95 O 5 CO This causes a high concentration O in de cage and in the container while the 5 concentration CO does not cause any reaction at the CO receptors in the animal lungs The SpO level in the blood becomes maximal After 1 min the Carbogen flow is stopped and 100 CO is introduced into the cage In this way the high concentration O is slowly replaced by CO This relatively slow replacement of O by CO is responsable for the reduction of stress for the animal Program 2 1 min Carbogen 1 min CQ This program is used for a very light anesthesia in mice and rats for very short procedures like f i bloodsampling manual Euth unit 06 2004 LI B 7 Manual control The different gasses can be introduced into the container cage separately from the programs It is NOT possible to introduce 2 gasses simultaneously 8 RESET To shut down a program or cancel manual control the particular button is to be pushed in again 9 Safety I is not possible to run 2 programs and or manual controls simultaneously Technical information Admissible temperature of surroundings 0 C till 50 C do not autoclave unit Max consumed power 40 Watt Voltage 220 230 Volt at 50Hz Malfunctioning Turn the UNO Euthanasia Unit off disconnect the unit from the electrical power and close all the va
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