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Contents
1. 00 00 001 i A Histogram foom Foun nas a a O WE k ex To exit the TS2 Software interface press either the close X or minimize _ buttons at the top right corner of the software see red circle above To power down the system exit the TS2 Software interface by pressing either the close X or minimize _ buttons at the top right of the software Then using the Windows Start menu select Shut Down Refer to the TS2 Software User Manual for further instructions on using the software iBox Spectra Imaging System 11 Selecting Emission Filters See the Installing Emission Filters section of this manual for instructions on installing individual emission filters Once all filters have been installed select the Lighting and Filters menu in the TS2 Software and click the Reset Wheel button under the Filters tab to realign and calibrate the filter wheel within the system Using the Overhead Epi Lighting To operate the built in overhead white light use the Lighting and Filters menu in the TS2 Software and select White under Epi Illumination Using the Warming Plate NOTE When the warming plate is plugged into the outlet in the darkroom and the darkroom is turned on the warming plate will receive a constant supply of power The warming plate must be turned off or unplugged after use as the software does not control the function of the warming plate Ensure that the warming plate on of2. TS2 screen Once the captured image appears on the screen touch the i button to open the image information screen and press the Set as White Balance button Touch the i button again to close the image information screen The system s white balance is now calibrated iBox Spectra Imaging System 13 Service Procedures Return Procedure A Returned Goods Authorization RGA number must be obtained from UVP Customer Service before returning any product Replacement Parts and Accessories To order accessories or replacement parts for the Box Spectra Imaging System contact UVP s offices Part Description Part Number Fuses Fuse 3 15A for Darkroom 56 0022 04 Qty 2 Recommended Excitation Filters Filter Assembly GFP 455 495nm Manual BioLite 38 0340 03 Contact UVP for Additional Excitation Filters Emission Filters Filter 515nm Long Pass 50mm Square 38 0364 01 Contact UVP for Additional Filters Troubleshooting No Power to the Darkroom or Warming Plate 1 Recheck the main power cord connection to the Box Spectra darkroom as well as the power cables between the darkroom and warming plate or optional handheld lamps 2 Check the fuses located at the back of the unit next to the power port A small flat head screwdriver or similar tool will be required Push the bottom tab of the fuseholder up until the fuseholder s bottom pops out Then push the top tab down until the top pops out The entire fu
3. darkroom Insert the knurled side of the fiber optic cable through the hole in the darkroom from the inside of the darkroom out leaving the two light guides in the darkroom Remove any existing plates or screws from the mounting bracket so that the epi light guide bracket appears as shown below Epi Light Guide Bracket iBox Spectra Imaging System Position the light guide in the mounting bracket as shown below Make sure the cable protruding from the light guide faces toward the front of the darkroom toward the darkroom door and that the light emitting portion of the light guide is facing toward the platform tray Cable faces the front of the darkroom Light emitting portion of the light guide is facing platform tray Light guide fits snugly in mounting bracket Attach the mounting bracket plate to the mounting bracket using two of the screws provided Make sure the tab on the plate faces up Mounting bracket plate with tab facing up Use 2 screws to secure plate to bracket 8 Insert the fiber optic bundle into the silver connector on the front of the BioLite Attach the cable to the silver connector with the black set screw Cable Connection iBox Spectra Imaging System 10 Using the System Powering Up the System Set the black power switch on the back of the system to the ON I position Once plugged into a surge protected wall outlet and the black power switch on the back of
4. on the warming plate surface 2 Using the TS2 Software click to turn ON the Live Preview button Adjust the exposure aperture zoom if applicable and focus controls on the bottom portion of the TS2 Software screen until an ideal image is visible Setting the White Balance Prior to capturing images using the iBox Spectra calibrate the system s white balance White balance calibration in essence is the process of removing unrealistic color casts so that objects which appear white in person are rendered white in the captured image This ensures that the white color in the image is as close to neutral white as possible and all of the colors in the image also appear true iBox Spectra Imaging System 12 To calibrate the white balance GV ae CA DO Place a focus target or business card on the warming plate inside the darkroom From the main TS2 screen turn on the epi white illumination as well as the Saturation Warning Using the Live Preview and Focus functions focus the image Remove the focus target or business card from the darkroom Place a sheet of clean white paper on top of the warming plate inside the darkroom and shut the darkroom door Access the Preferences menu from the main TS2 screen and ensure that the Autocapture Image Contrast is set to Best Press OK to close the Preferences menu After ensuring that the epi white illumination is still on touch the Auto Capture button on the left side of the main
5. the main power cable into the back of the darkroom and the other end into a surge protected power outlet Note It is recommended to leave the power switch on the back of the system in the ON position except when the system will not be used for an extended period of time one day or longer Note Do not position the system so that it is difficult to access the power cable and operate the main power switch at the back of the unit 2 If installing optional epi handheld lamps place the lamps in the brackets located at the top of the darkroom Plug the handheld lamps into the outlets provided inside the darkroom Place the power switches located on the outside of the handheld lamps in the ON position Installing the Warming Plate 1 Place the warming plate inside the darkroom on top of the Roll Out Warming Plate Tray 2 Plug the jumper cord into the outlet located in the upper left back panel of the darkroom 3 Plug the other end into the warming plate NOTE When the warming plate is plugged into the outlet in the darkroom and the darkroom is turned on the warming plate will receive a constant supply of power iBox Spectra Imaging System Installing Emission Filters To install the 50mm emission filter and any other emission filters 1 Carefully remove the filter from the protective plastic case holding the filter at the edges to avoid placing fingerprints on the glass surface 2 The filter wheel is located on the right sid
6. the system is placed in the ON I position the iBox Spectra is always powered on Press the Computer Power button on the front of the system to power on the internal Power Indicator Lights Computer P r computer touch screen and access controls for the Ston camera lenses lighting and other functions The Power Indicator Lights on the front of the unit indicate when the system s main power and optional ultraviolet illumination are on Main Power will illuminate green when the black main power switch on the back of the unit is in the ON I position this does not indicate that the internal computer or touch screen are on Ultraviolet On will illuminate red when any optional ultraviolet illumination is active within the darkroom The POWER button will illuminate blue when the internal computer and touch screen are on Note The black main power switch on the back of the unit must be in the ON I position in order for the internal computer and touch screen to function Note It is recommended to leave the power switch on the back of the system in the ON position except when the system will not be used for an extended period of time one day or longer Operating the TS2 Software Interface Upon startup the internal computer will proceed through the boot up process When complete the screen will display the Windows desktop The TS2 Software screen similar to the one below will automatically open shortly thereafter
7. al Source is a 150 Watt quartz halogen visible light source designed for use with ferrule fiber optic bundles It features a closed optical path to tightly control the output spectrum allowing consistent and repeatable measurements with superior signal to noise The BioLite features a six position dimmer permitting variable intensity The user inserts the excitation filter into the slot at the front of the BioLite to control the wavelength of light emitted TS2 Software Image analysis and hardware functions for the iBox Spectra are controlled by the TS2 Touch Screen Software interface In addition to image preview capture and save functions the TS2 Software controls camera and lens functions exposure aperture focus and zoom image printing lighting and filter selection and other user preferences For additional information refer to the TS2 Software User TS Share Sorgen Manual included with the system or available online at http uvp com manuals iBox Spectra Imaging System 6 Focus Target The UVP Focus Target provides sharp detailed images to aid in adjusting the lens and camera settings for ideal imaging results Focus Target Optional Equipment UVP offers a variety of optional equipment to support the needs of varying laboratory environments Refer to Replacement Parts and Accessories at the end of this manual for optional equipment part numbers Thermal Printer The thermal printer provides archive qu
8. ality 256 grayscale prints and five optional cost effective print sizes Thermal Printer Anesthesia Unit The anesthesia system is an optional component designed to safely anesthetize up to five small animals at a time inside the darkroom The patented non rebreathing technology safely prevents backflow of gases into the darkroom Anesthesia units are available with either isoflurane or sevoflurane as well as with or without oxygen regulators Contact UVP for ordering information Anesthesia kit iBox Spectra Imaging System f Setup Instructions Components When unpacking the iBox Spectra the following items will be included iBox Spectra darkroom Manual BioLite MultiSpectral Light Source Warming plate 455 495nm excitation filter and 515LP emission filter UVP flash drive Power and USB cables Keyboard and mouse Supporting documentation ON PED A W S When unpacking and setting up the system two people are required to move the darkroom Place the darkroom on a flat surface which can provide adequate support for up to 100 pounds WARNING Do not attempt to perform any setup procedures while the system is plugged in or powered on unless otherwise instructed CAUTION Do not install the system in areas with high moisture dust or high temperatures Keep the equipment away from motors or any other large magnetic equipment apparatus This system is designed for indoor use only Connecting the Power Cables 1 Plug
9. e of the darkroom Press in on the slide out filter wheel door and the door will pop out slightly Pull the filter wheel assembly out until it stops 3 Manually rotate the filter wheel to the desired position Place the included filter in Position 1 NOTE Before placing the filter in the filter wheel ensure that the text on the edge of the filter is positioned so it is right side up when facing the installer 4 Note the position of all installed filters for entry into the Lighting and Filters menu in the TS2 Software refer to the TS2 Software User Manual for more information 5 Once all filters have been loaded slide the filter wheel assembly back into the system Press the filter wheel assembly door until the assembly locks into place Additional and replacement emission filters are available through UVP Refer to the Replacement Parts and Accessories section of this manual for ordering information Installing the BioLite MultiSpectral Light Source 1 Plug the power cord into the receptacle on the back of the BioLite and the other end into a surge protected power outlet Filter Cartridge Insert the filter cartridge into the filter port NOTE Filters and their cartridges are directional Make sure that the label is facing toward the front of the BioLite Because of the intense light of the BioLite only tempered interference filters should be used Remove the plug from the access port at the upper left side of the
10. f switch is turned on The warming plate will show the temperature of the warming plate in the display In several minutes the warming plate will reach 37 C NOTE The warming plate has an over limit switch that prevents the unit from exceeding 41 C Should the unit reach 41 C the plate must be returned to a temperature of 25 C before the unit reheats NOTE Allow the warming plate to reach room temperature prior to operating If the unit is extremely cold when switched on the warming surface temperature can exceed the preset temperature indicated on the LCD display Using the Sample Viewer Window The Sample Viewer Window built into the darkroom door allows users to view the interior of the darkroom without opening the darkroom door To open the Window press firmly on the top of the Window cover to release the pressure sensitive clasp and open the viewer The Window glass provides a clear view to the warming plate surface for sample viewing without opening the darkroom door In the case that ultraviolet illumination is being used in the darkroom the Viewer Window also blocks the user from harmful exposure to UV light NOTE To avoid light leakage close the Sample Viewer Window prior to capturing a light sensitive image Image Focusing Prior to capturing an image prepare the image focus 1 Turn on the white epi illumination and place the Focus Target see the Focus Target section of this manual for more information
11. ging System 3 Introduction The iBox Spectra Small Animal Imaging System is a high resolution imager designed as a quick screening device for accurate repeatable imaging of small animals The self contained imaging system contains a built in 15 6 touch screen and computer as well as a light tight darkroom with TS2 Software for image acquisition In addition the iBox Spectra comes equipped with a warming plate to help maintain the animal at a constant 37 C The iBox offers a color camera and motorized lens for in vivo applications providing real time live preview images Included as standard with the system is a Manual BioLite which offers a direct lighting source using fiber optic bundles to tightly control the output spectrum for consistent repeatable measurements The Manual BioLite utilizes matched excitation 455 495nm and emission 515 long pass filters for visualization of small animals The darkroom has a sample viewer window built into the darkroom door integrated overhead epi white lighting for sample placement and a motorized five position slide out emission filter wheel Images can be saved to the internal hard drive to an external USB storage device or to a wired or wireless network drive for later analysis or enhancement for publication iBox Spectra Imaging System 4 System Components Refer to the packing slip and pictured components for specific parts and components included with the system Power Indicator Ligh
12. iBox Spectra Small Animal Imaging System Installation and User Instructions LUV Pp An Analytik Jena Company UVP LLC Ultra Violet Products Ltd 2066 W 11th Street Unit 1 Trinity Hall Farm Estate Upland CA 91786 Nuffield Road Cambridge CB4 1TG UK Phone 800 452 6788 Phone 44 0 1223 420022 Fax 909 946 3597 Fax 44 0 1223 420561 Web Site www uvp com 81 0304 03 Rev D iBox Spectra Imaging System 2 Table of Contents Table Ol COMMIS oss e e eee o eas 2 INTOducION ita o A e Mane LS eae eee 3 SYSTEM ComponeniS srie dd ica 4 ODIO Mal EGUID IIS ollo lc 6 Setup TSTEUC TIONS ccs tt el dd do lo ied di a dol 7 o a 7 InStallingthe Warming Plate 7 Stalin EMmISSION FIEIS aa a a E E io ironia 8 Installing the BioLite MultiSpectral Light SOUFCE ooonncccnnccccononncnonnncnnnnonncnnnnonononnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnonnncnonnnanenons 8 USING TN SM id 10 Hd NS E tepenase cata nlnneataat anton steaduede areca nectasdeearenicesa 10 Operating the TS2 Software eta e e de de do E 10 Selecting Emis Som PIES O o Ta 11 Using the Overhead Epi LIA e aa o 11 BE o O Mise Seems sania aa 11 Usina he Samm ple Viewer WAC OW rss ad 11 Mage FOCUS MO o es o de cis de e eta lo e cies al ach oe cos e es lol done 11 EU O re 11 Service Procedures oi o cia 13 Ai a AAA A A soegeaun tes 13 Replacement Pans AnG ACCESSONES it toscas 13 THOUDIESMOO IM ie e il do li and e eb 13 TECHNICAL Uppsala 14 iBox Spectra Ima
13. ll camera settings are factory pre set for optimum performance when viewing samples under low light conditions Contact UVP Technical Support prior to making any adjustments to the camera settings Darkroom The darkroom is light tight to provide optimal imaging conditions Darkroom features include Epi overhead LED white light Roll out warming plate tray Sample viewer window built into the darkroom door Five position emission filter wheel Brackets and built in power ports for installing optional epi illumination lamps Safety interlock switch with manual override to disable UV when darkroom door is opened LCD Touch Screen The iBox Spectra contains a fully integrated 15 6 inch color touch screen The touch screen allows the user to perform a variety of tasks including previewing capturing saving and printing images as well as selecting preference options without the use of an external mouse or keyboard For users who prefer not to use the touch screen interface an external keyboard and mouse are included as standard These components can be plugged into any available system USB port Warming Plate The warming plate houses electrical heating elements which maintain the surface of the warming plate at a constant temperature of 37 C to keep animals warm during the imaging process The warming plate plugs into a dedicated port inside the iBox darkroom BioLite MultiSpectral Light Source The Manual BioLite Multispectr
14. seholder can now be pulled out Inspect the thin wire within each glass fuse to see if there is a break in the wire If so replace the fuse s If fuses are blowing repeatedly contact UVP Technical Support for additional troubleshooting Error Messages Appear on the Screen 1 An error message that is related to the TS2 software interface or Microsoft Windows may appear on the screen If the message is related to Microsoft Windows such as a reminder to activate or update the copy of Windows please contact your system administrator for assistance 2 If an error message appears repeatedly and your system administrator does not recognize it as a Microsoft Windows error contact UVP Technical Support for further assistance Care and Cleaning Use only mild soap or detergent solution for cleaning Do NOT use oil or petroleum based cleaners for the cabinet Ensure that the system is turned OFF and unplugged during cleaning iBox Spectra Imaging System 14 Technical Support UVP offers free lifetime technical support on all of its products and software Should you have any questions regarding the product s use operation or repair contact UVP s offices at the locations below or visit WWW UVP COM If you are in North America South If you are in Europe Africa the America East Asia or Australia Middle East of Western Asia Call 800 452 6788 or 909 946 Call 44 0 1223 420022 and ask for 3197 and ask for Technical Suppor
15. t Customer Service during regular during regular business days between business days between 9 00 am and 7 00 am and 5 00 pm PST 5 30 pm E mail your message to vai l Fax Customer Service and send it to Fax Customer Service and send it to 909 946 3597 44 0 1223 420561 Write to Ultra Violet Products Ltd Unit 1 Trinity Hall Farm Estate Nuffield Road Cambridge CB4 1TG UK Write to UVP LLC 2066 W 11 Street Upland CA 91786 USA iBox is a registered trademark of UVP LLC Spectra and BioLite are trademarks of UVP LLC
16. ts 15 6 LCD Touch Screen with Built In Computer Camera and Lens BUVP Built Into Darkroom Five Position Slide Hood Out Emission Filter Wheel Main Power Button Built In Overhead White Lights Safety Interlock Switch amp Override Sample Viewer Window USB and SD Card Access Port BioLite Access iBox Wide Access Door spectra 5 Roll Out Warming Plate Tray Specifications Power Requirements 100 115V 50 60Hz 3 1 Amps at 120 Volts 230V 50 60Hz 1 55 Amps at 230 Volts Mains supply voltage fluctuations are not to exceed 10 percent of the nominal supply voltage Pollution Degree 2 Installation Category II Altitude Up to 2000m Ambient Temperature 5 C to 40 C Humidity Maximum relative humidity of 80 for temperatures up to 31 C decreasing linearly to 50 maximum relative humidity at 40 C Built In Touch Screen Computer Operating System Windows 7 64 Bit Connectivity Ports 2 USB 1 SD SDHC MMC side of system 4 USB VGA and Ethernet rear of system Wireless Networking Capability 802 11 b g n USB Flash Drive Capacity 2 GB minimum Internal Hard Drive 250 GB minimum Software TS2 Touch Screen Software iBox Spectra Imaging System 5 Camera and Lens The iBox Spectra is equipped with the MultiCam 310C camera a scientific grade color CCD camera with a resolution of 2 0MP 1600x1200 The MultiCam 310C is equipped with a 12 5 75mm f 1 2 zoom lens A
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