instruction manual for bench top centrifuge
Contents
1. INSTRUCTION MANUAL FOR BENCH TOP CENTRIFUGE Z 300 Z 400 Labnet International PO Box 841 Woodbridge NJ 07095 Phone 732 417 0700 Fax 732 417 1750 Z 300 Z 400 Jan 2002 General Information 1 1 Precautions and hazards 1 2 Description 1 3 Safety standards 1 4 Technical data Z 300 Technical data Z 400 1 5 Accessories supplied with each centrifuge unit 1 6 Warranty Installation 2 1 Unpacking the centrifuge 2 2 Transport 2 2 Required space 2 3 Installation How to install and load a rotor 3 1 Mounting and securing a swing out rotor 3 2 Mounting and securing an angle rotor 3 3 Mounting and securing a hematocrit rotor 3 4 Overloading of rotors 3 5 Removing of the rotor Operation 4 1 Power switch 4 2 Lid release 4 3 Lid lock 4 4 Preselection of speed 4 5 Preselection of operating time 4 6 Keyboard Starting the centrifuge quick key 4 7 Key stop Temperature 5 1 Temperature Safety facilities 6 1 Imbalance Service and Maintenance 7 1 Service and inspection of the centrifuge 7 2 Cleaning the centrifuge 7 3 Cleaning the centrifuge after breakage of glass tubes bottles 7 4 Disinfection Breakdown 8 1 Emergency lid release 8 2 Check list Trouble shooting Appendix 9 1 Chart for determining g values General Information 1 1 Precautions and hazards Before putting the centrifuge into operation please read this instruction manual carefully The centrifuge must not be operated by unqualifi2. eye measuring and insert them into the tube holes of the rotor The difference in weight between the tubes should not exceed 2 3 grams It is also allowed to operate a 6 place rotor with 2 or 4 loaded tubes only but it is important that the 2 or 4 occupied bore holes are opposite each other see figure 1a and 1b When using tubes without lid in angle rotors please note that the tubes should only be filled partly i e 60 75 of the maximum tube volume Incorrect Correct sketch 3 Mounting and securing a hematocrit rotor Clean the motor shaft and the rotor mounting hole with a piece of cloth and place the rotor on the motor shaft ensuring that the pins align correctly with the rotor slots Hold the rotor with one hand and secure the rotor to the shaft by turning the rotor nut counter clockwise see figure 7 When loading the rotor make sure that the sealing is in good condition The rubber should not be brittle dirty or damaged by the capillaries If necessary replace the sealing order number of the sealing 23 5147 To remove or to close the rotor lid press both locking bolts together Overloading of rotor The max load permitted for a rotor which is determined by the manufacturer as well as the max speed allowed with the rotor see indications on the rotor itself must not be exceeded The liquids with which the rotors are loaded should have an average homogeneous density of 1 2 g per ml or less when the rotor
3. key stop if you want to interrupt a centrifuge run The centrifuge decelerates according to the fix adjusted brake intensity You can not change the brake intensity 5 Temperature Features 5 1 Temperature During centrifugation heat is generated by air friction between the rapidly spinning rotor and the air inside the rotor camber The temperature rise depends on the rotor swing out or angle rotor bucket type ambient temperature running time and the speed of the rotor The continous air flow through the centrifuge housing is restricting the temperature rise of the samples to the standard value of 40 C with each rotor even at maximum speed 6 Safety facilities 6 1 Imbalance In case of unequal loading of opposite buckets tube racks or tube holes the operation will be interrupted during the acceleration phase The rotor will be decelerated to standstill Additionally the error message ERROR appears on the preset display speed If the actual display speed shows error no 1 the difference in weight of the samples is too big Fill the tubes and load the rotor as described under point 3 If the actual display speed shows error no 2 there can be several reasons for The imbalance switch is not adjusted correctly The imbalance switch is defective 7 Service and Maintenance Service and inspection of the centrifuge Centrifuge service and inspection should be done regularly and only by authorize
4. kg dm 4 000 Nm Electrical connection Current Connected load 230 V 50 Hz 1Ph 120 V 60 Hz 1Ph 3 8 A 40A 650 Watt 480 Watt Interference suppression VDE 0871 interference degree B Service dept at Hermle 0 74 26 96 22 55 Address of service Address of agent 1 5 1 6 Accessories supplied with each centrifuge unit 2 Replacement fuses 1 Instruction manual 1 Tool for removing the rotor Warranty The centrifuge has been subjected to thorough testing and quality control In the unlikely event of any manufacturing faults occuring the centrifuge and rotors are covered by warranty for a period of one year from date of delivery This warranty becomes invalid in case of wrong operation use on non appropriate spare parts or accessories and non authorized modification of rotor or centrifuge The manufacturer reserves the right for any technical modifications of the product in respect to technical improvement Installation 2 1 Unpacking the centrifuge The centrifuges are supplied in a carton Remove the tightening straps Open the carton and take the centrifuge together with the styropore packing out of the carton The instruction manual and the accessories mentioned under 1 5 should be kept with the centrifuge Transport Avoid impacts during transportation and do not drop the unit to perserve it from being damaged Required space The centrifuge should be insta
5. can preset the operating time with the knob 3 During the run and with the centrifuge lid closed you have to press additionally key preset 1 to change operating time during the run The preselected running time will be indicated on the time display 5 At the end of a run the preset operating time will be kept for further runs For continuous runs turn the knob clockwise to the limit stop The continuous run will be indicated on the digital indication with two minus signs You can stop a continuous run with key stop 4 6 Keyboard Starting the centrifuge quick key Key quick for short time runs Key lid to open the centrifuge lid If the green LED of the key lid is lighting the lid is closed correctly Key start to start the centrifuge Key stop to stop the centrifuge before the preset operating time has expired or to stop the centrifuge at continuous run Figure 6 Starting the centrifuge The rotor has to be fixed correctly and completely loaded see point 3 Close the centrifuge lid As soon as the green LED of the key lid is lighting the centrifuge can be started Therefore press key start quick key Short time runs For short centrifuge runs you can start the run with the key quick Press the key quick The centrifuge starts and keeps running as long as you press the quick key The operating time will be indicated in seconds on the digital indication time Key stop Press the
6. center of the rotation axis fo the bottom o outermost portion of the test tube should be used ACF is expressed relate to ihe force of the earth s gravity BB AL atildiddila util ole hi a a HERMLE centrifuges the name that s setting the standard 5 8 Lb Peso d penn a 1 Peta telelatel a seadae r E k TECTITI m TTT nye E p iT TTT
7. d and qualified personnel Use only original spare parts Maintenance and cleaning Maintenance The maintenance of the centrifuge confines esentially to keeping clean the rotor chamber the rotor and the accessories as well as to the regular lubrication at the rotor inserts bolts of the swing out rotor Vaseline available in nearly each store is the most suited lubricat However please ensure that the vaseline has to be free of resin and acid The use of lubricants containing molykote and graphite is not allowed Please pay special attention to anodized aluminium parts Breakage of rotors can be caused even by slight damages In case the rotor buckets or tube racks get in touch with corrosive liquids the respective spots and parts have to be cleaned carefully Corrosive liquids are for example alkaline soup solution alkaline amino strong acids solutions containg heavy metals waterfree and chlorinated solvents salt solutions e g sea water Cleaning The purpose of a thorough cleaning is beside hygienical reasons the avoidance of corrosion by soiling In order to avoid the damage of anodized parts such as rotors reduction plates ect only neutral cleaning agents with a ph value 6 8 should be used Never use an alkaline cleaning agents ph gt 8 After cleaning please ensure that all parts are dried thoroughly by hand or in a warm air cabinet max temperature 50 C It is recommended that all anodized aluminum par
8. ed correctly The green control lamp of the key lid will light as soon as the lid is closed correctly When the rotor starts accelerating the control lamp of the key lid extinguishes and the lid cannot be opened 4 4 Preselection of speed RCF You can preset the speed between 250 rpm amd 13500 rpm in steps of 250 rpm ec rateta e goa00gn0n00g000 booooagoocoaoad oo0000000000 bossacaaccas oooo0og00nca0 ooqan0ognaoago Ga000g0nogo0gan booog0gogocgagga oooo0gogogogog When the centrifuge lid is open you can preset the required speed or rcf with the knob 2 If the centrifuge lid is closed and during the run the speed can be changed as follows Press key preset 5 hold it and at the same time change speed with the knob 2 The preselected speed will be indicated in the speed display 4 The centrifuge will accelerate only to the max allowed speed of the inserted rotor The allowed max speed for all rotors please see table under 220 86 V01 220 27 V02 220 50 V05 220 81 V05 220 82 V01 220 59 V06 max Speed 3 500 rom 4 000 rpm 3 500 rom 3 500 rpm 6 000 rpm 13 500 rpm with bucket 611 015 3500 rom 220 72 V04 220 50 V05 220 81 V05 220 82 V01 220 59 VOG max Speed 4 000 rpm 3 500 rpm 3 500 rpm 6 000 rom 13 500 rom 4 5 Preselection of operating time You can adjust the desired operating time between 1 and 60 minutes or hold With the centrifuge lid open you
9. ed persons not familar with the correct use and intended purpose of the machine Please use only the original spare parts For personal and enviromental safety special attention has to be paid to the following precautions The Hermle Centrifuges are neither explosion proof nor intert gas shielded and should therefore never be operated in explosion hazardais locations Never stay in the safety zone of 30 cm around the centrifuge or deposite dangerous goods inside this zone during centrifugation The centrifugation of the flammable or explosive or radioactive samples is not allowed Further more do not spin samples reacting chemically with each other with high energy when explosed to air Never spin toxical or phatological material without adequate safety precautions i e centrifuging of buckets tubes without or with defective hermetic sealing is not allowed The enduser is engaged to performe appropriate disinfection procedures in case dangerous goods have contaminated the centrifuge or its accessories The general universal laboratory precautions should be observed in case infection materials are centrifuged If necessary please contact your local security officer It is prohibited to run the centrifuge with rotors not suited for this centrifuge model Under no circumstances the lid at the centrifuge should be opened while the rotor is still turning respectively running with a speed of more than 2 meter per second The following rules mus
10. he general requirements set by German law for medical apparatus MedGV group 3 The following standards have been considered for the production of our centrifuges Accident prevention rules for centrifuges UVV VBG 7z Accident prevention rules for electrical equipment amp installations UVV VBG 4 International Standart IEC 1010 1 and IEC 1001 2 D European Standart PR EN 61010 1 and PR EN 61010 2 2 Electrical interference suppression according to interference degree B as per VDE 0871 1 4 Technical data Manufacturer Hermle Labortechnik GmbH Type Z 300 Dimensions Width Depth Height 35 5 cm 33 cm 47 4 cm Weight 27 kg Noise level 65 dB A Max speed Max Volume Max RCF Admiss density Admiss kinetic energy 13 500 rpm 400 ml 17310 xg 1 2 kg dm 3 052 Nm Electrical connection Current Connected load 230 V 50 Hz 1Ph 120 V 60 Hz 1Ph 3 8A 40A 580 Watt 460 Watt Interference suppression VDE 0871 interference degree B Service dept at Hermle 0 74 26 96 22 55 Address of service Address of agent 1 4 Technical data Manufacturer Hermle Labortechnik GmbH Type Z 400 Dimensions Width Depth Height 41 cm 37 5 cm 56 5 cm Weight 33 kg Noise level 65 dB A Max speed Max Volume Max RCF Admiss density Admiss kinetic energy 13 500 rpm 1 000 ml 17310 xg 1 2
11. is running at maximum speed To spin liquids of a higher density the speed should be reduced according to the following formula 3 2 Reduced speed N ied higher density value x max speed N na Example 2 x 4000 3360 rpm In case of any questions please contact the manufacturer 3 5 Removing of the rotor A Take off the rotor lid and hold the rotor with one hand Turn the rotor nut clockwise until the rotor is loose and take the rotor vertically off the shaft ATTENTION Never operate the centrifuge with rotors or buckets which show any signs of corrosion or mechanical damage Never operate with strongly corrosive materials which could damage rotor and buckets 4 Operation 4 1 Power switch Connect the cord plug to the appropriate wall socket After connecting the digital displays will light up The control panel is equipped with a stand by function Lid release When the green control lamp of the key lid lights the rotor stands still and centrifuge lid is ready to open Press key lid see figure 5 to open the lid The green control lamp will extinguish as soon as the lid will be opened or the unit started Control lamp rotor is stationary Shows that the lid is closed correctly Key lid To open the centrifuge lid Figure 5 4 3 Lid lock Close the centrifuge lid after the rotor has been fixed correctly as described The centrifuge can only be started when the lid is clos
12. it is missing Reason A magnet of the indicator ring 1s missing Action Check indicator ring and call service Error no Rotor is not mentioned in the rotor chart Reason Rotor s not authorized for this unit Action Insert only rotors authorized for this unit Error no Power failure Reason Power failure while rotor s in motion Action Open and reclose the lid restart the centrifuge Error no Radius correction Reason Radius correction value too big Action Adjust the radius correction to the correct value Error no Relay for the frequency converter cannot be released Reason Defect on the power board Action Call service Error no Memory failure Reason Internal or external memory failure Action Restart the unit if the failure occurs again call service Error no Overspeed Reason Overspeed sensor or engine speed sensor defective Action Call service Error no Engine speed sensor signal is missing Reason Engine speed sensor defective or parting of a cable at the sensor Action Call service Error no 70 Interface of the frequency converter Reason Communication of controller power board interface cable and frequency converter s not working Action Call se
13. lled on a rigid even surface The Z 300 and Z 400 should only be operated on a stable laboratory table cabinet etc Balance the centrifuge with a spirit level To guarantee the necessary heat dissipation the unit has to be placed in that way that there is a space of minimum 15 cm on each side of the unit Never place the centrifuge in positions subject to excessive heat e g strong sunlight as the performance of the unit is based upon an ambient temperature of 23 C Attention The new safety rules require a safety operation cycle of 30 cm around the centrifuge Mark this area to indicate that no person and now dangerous material e g comprising inflammable or infectious liquids are present during centrifugation Installation Check that the power supply corresponds to that on the manufacturer s rating label which is mounted on the rear panel then connect the power cord to the centrifuge and the socket The line voltage circuit breaher is maximum a 16 Amp type K slow release for commonly used instruments That an ermergency switch is installed out side the room to disconnect the power supply in case of a troubled run The power switch is at the rear side of the unit Switch it on The digital indications on the display are lighting up Press key lid You can open the centrifuge lid now How to install and load a rotor 3 1 Mounting and securing a swing out rotor Clean the motor shaft as well as the rotor mounti
14. lows Switch off the centrifuge and unplug the power cord There is a plastic plug at the left side of the centrifuge housing Behind that plastic plug there is a red cord Remove the plastic plug and pull the red cord The lid can then be opened see figure 15 figure 15 8 2 Check list Trouble shooting The error message will be indicated by a certain number on the digital speed display At the same time ERROR appears on the preset display There is a distinction between two different kinds of errors The digits on the indication speed have the following meaning Error no 1 49 Forced stop If one of those errors occurs the rotor will be braked from the preset speed to 0 As soon as the rotor has stopped the error message can be reset by opening and closing the centrifuge lid Error no 50 99 Emergency stop If this occurs the frequency converter will be switched off This means that the rotor will be stopped brakeless To reset the error message you have to plug off and plug in the power cord If the unit stops due to an error indication you should restart the unit to check if the error occurs again The error numbers which are not listed in this chapter are not in use at the time of publication and they are reserved for future use in widening the error recognition program Error no 1 Imbalance Reason ncorrect loading of the rotor Action Balance your samples Reason ncorrect adju
15. ng hole with a piece of cloth and place the rotor on the motor shaft ensuring that the pins align correctly with the rotor slots see sketch 1 Z lt Incorrect Correct sketch 1 Hold the rotor with one hand and secure the rotor to the shaft by turning the rotor nut counter clockwise When loading the buckets and tube racks you should proceed according to sketch 2 It is very important to load the rotor with the complete set of buckets tube racks The bucket insert bolts of the rotor should be regularly greased with silicone grease Fill the tubes equally by eye measuring and insert them into the tube holes or tube racks The difference in weight between the buckets should not exceed 10 grams Ssss C800 Incorrect Correct sketch 2 It is also allowed to operate for example a 4 place swing out rotor with 2 loaded and 2 unloaded buckets but it is important that the loaded buckets are then opposite each other Mounting and securing an angle rotor Clean the motor shaft as well as the rotor mounting hole with a piece of cloth and place the rotor on the motor shaft ensuring that the pins align correctly with the rotor slots see page 6 sktech 1 Hold the rotor with one hand and secure the rotor to the shaft by turning the rotor nut counter clockwise ATTENTION Before operation secure the rotor lid to the rotor by pressing the snap connector on the rotor nut Load the rotor according to sketch 3 Fill the tubes equally by
16. rvice Error no 82 83 Cutoff power board frequency converter Reason Overcurrent or undervoltage due to power supply fuctuations Action Restart the unit take care that the power supply is stable Error no Overtemperature at the driving Reason Temperature at the converter or motor too high Action Switch off the centrifuge Wait for about 15 min and switch the unit on again Error no 85 87 Failures Reason Internal defect Action Call service Error no Emergency lid release Reason The centrituge lid has been opened by the emergency lid release during the run Action Close centrifuge lid Danger of accident Reason Control switch of the lid lock is defective Action Call service Error no 94 Voltage loss during run Reason The power supply is below the tolerance for a short moment Action Wait till stillstand of the rotor Open centrifuge lid after the yellow LED lid is lighting Switch off and on the main switch Appendix 9 1 Chart for determining g values RELATIVE CENTRIFUGAL FORCE Speed fin BAM 20 000 8 Relative Centrifugal Force The relative centrifugal force G Force can be estimated using the chart on thes page or by applying the following formula ge naxe oo p whe f adus m Centimeters f speed in APM The radius from the
17. stment of the imbalance switch Action Imbalance switch has to be readjusted call service Error no 2 Permanent imbalance signal Reason Position of the imbalance switch not correct Action Imbalance switch has to be readjusted call service Reason Imbalance switch is defective Action Imbalance switch has to be replaced call service Error no 10 Overtemperature in the rotor chamber more than 50 C Reason Breakdown of the refrigeration system Electronic failure Action Call service Reason Temperature sensor defective Electronic failure Action Call service Error no 11 Temperature sensor Reason Short circuit at the temperature sensor or at the sensor cable Action Call service Reason Chamber temperature s too low below 25 C Action Solenoid valve is not working Call service Error no 20 No rotor identification Reason No rotor inserted Action Insert rotor into the unit Reason Rotor identification sensor defective Action Call service Reason nserted rotor has no indicator ring Action Use a correct rotor Reason Rotor s not fixed correctly to the motor shaft Action Insert rotor correctly The pins has to align correctly with the rotor slots see chapter 3 Error no 21 Start B
18. t be strictly adhered to Do not operate the centrifuge in case it is not installed correctly Never operate the centrifuge in a dismounted state e g without covering sheet metal Do not run the centrifuge when electrical or mechanical assembly groups have been tampered with by unauthorized persons Never use accessories such as rotors and buckets which are not exclusively approved by Hermle Labortechnik GmbH except commercially available centrifuge tubes of glas or plastic Do not spin corrosive samples which may cause damages in material and impair the mechanic resistance never operate the centrifuge with rotors or buckets which show any signs of corrosion or mechanical damage The manufacturer is only responsible for the security and reliability of the centrifuge if The unit is operated according to the instruction manual Modifications repairs and new adjustments are performed by HERMLE authorized persons and the electrical installation of the location where the centrifuge is operated corresponds to the IEC regulations Description The model Z 300 and Z 400 are a universal centrifuge which covers many fields of applications by offering a wide range of accessories The centrifuges are equipped with the Hermle Standart Control panel Speed and running time are set with easy to use control knobs The precise parameters selected are shown on the large digital LED display Safety standards The centrifuge corresponds to t
19. ts are regularly treated with anti corrosion oil so that their durability will be increased and the corrosion risk reduced By humidity and non hermetically closed samples condensate mist may be formed The condensate mist has to be removed regulary with a cloth from the rotor chamber 7 3 Cleaning of centrifuge after breakage of glass tubes glass bottles With high g values there is a possibility that tube breakage will occur Should this happen the centrifuge rotor buckets adapters and the rotor chamber must be thoroughly cleaned and all broken particles removed immediately If this is not done they could scratch the protective coating of the rotor If the rotor chamber has not been properly cleaned this will produce a fine black dust which can cause significant damage to the centrifuge chamber rotor buckets and the samples Disinfection If for example through tube breakage infectious material is spilled into the centrifuge the rotor rotor chamber buckets etc should be disinfected Rotor and swing out buckets must not be autoclaved Rotor and rotor chamber should then be treated with a neutral disinfection agent for example on formalin basis A disinfectant spray should be used to thoroughly clean the rotor chamber rotor bucket tube rack etc 8 Breakdown 8 1 Emergency lid release In case of power failure or any malfunction the lid can be opened manually to protect your samples Please proceed as fol
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