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C11440-22CU C14400-22CU01 Instruction manual
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1. 52 12 2 1 WHEN USING COOLING WATER OTHER THAN RECOMMENDED 52 TROUBLESHOOTING CHECKLIST eese 53 13 1 IMAGE IS NOT 53 13 2 ALTHOUGH IMAGES ARE TRANSFFERED 53 SPEGIFILAT ION 54 14 1 CAMERA nennen 54 14 2 CONDENSATION 57 14 3 SPECTRAL RESPONSE CHARACTERISTICS TYP 58 14 4 INTERFACE SPECIFICATIONS nennen nnns 59 14 4 1 CAMERA LINK INTERFACE 59 14 4 2 OUTPUT TIMING SPECIFICATIONS 000 62 DIMENSIONAL OUTLINES unse ee 66 C ee NUN 66 192 1344307225 UU T za E Irre 67 WARRANT E 68 CONTACT INFORMATION ee 69 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 4 OVERVIEW C11440 22CU C11440 22CUO1 is equipped with the new scientific image sensor an advanced CMOS device that realizes the multiple benefits of high resolution high readout speed and low noise all at once The camera provides 4 0 megapixels resolution at 100 frame s and up to 25 655 frame s by sub array readout while achieving 1 0 electrons median 1 6 electrons r
2. u CL Port C Data Ht rer mer ert CL Port D Data reo mau CL Port E Data tet wat zet heor freos CL Port Data reu wu 7 CL Port G Data et i mst mar est c eg CL Port H Data reu teu msu meu reso r2ossufrzos CL Port I Data t ren eat Haosa t ito CL Port J Data tu tou mau mou resu zoos uto uito HAMAMATSU 65 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 15 DIMENSIONAL OUTLINES 15 1 C11440 22CU Unit mm 6550 3 66 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 15 2 C11440 22CU01 Unit mm EP HAMAMATSU 67 11440 22 0 11440 22 001 Instruction manual Ver 1 5 16 WARRANTY Hamamatsu Photonics have fully inspected this system and checked that its performance conforms to specifications In the unlikely event of breakdown or other malfunction contact Hamamatsu subsidiary or local distributor 1 Unless otherwise stated by Hamamatsu subsidiary or local distributor this system is under warranty for 24 months from the delivery date Degradation with cosmic rays the radiation X rays gamma rays UV light etc of FL 400 is excep
3. Te Tee T Te CL Lvalid CL Port A Data CL Port B Data CL Port C Data CL Port D Data CL Port E Data CL Port F Data CL Port G Data CL Port H Data CL Port Data CL Port J Data HAMAMATSU 63 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 64 3 4x4 binning readout Camera Link Timing 4x4 Binning C L Fvalid C L Lvalid C L Fvalid C L Lvalid 255 V256 TxClock 85 MHz CL Fvalid CL L valid V254 V509 V1 103 clock 311 clock V255 CL Port A Data L Lower byte tot mor reor CL Port B Data U Upper byte wu Hsu mou msu H5000 voto TN ps pe es pes E Teen Dac Dec esc Te s E m Dc Dec sce e Te os 2 MEN CK 77177122 Duc Tec ee c Tes ios E i HAMAMATSU VO C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 4 Sub array readout Camera Link Timing Vertical Sub Array 2 same size regions readout C L Fvalid C L Lvalid C L Fvalid V o1 w V 02 Wo1 w 1 Wo1 2 V o2 w 2 Wo1 1 V o2 w 1 _ Vo V o2 w C L Lvalid Tx Clock 85 MHz 1 CL Fvalid 410 clock _ CL Lvalid V offset width Top region V offset2 Bottom region CLPortAData Lilowerbyte ror ret mor teor CL PortBData U Upper byte mu mou msu
4. 1 End the imaging or transmission of image data with the application software 2 Turn off the circulating water cooler In the case of water cooling 3 Turn off the camera and peripheral devices HAMAMATSU 23 11440 22 0 11440 22 001 Instruction manual Ver 1 5 10 DESCRIPTION OF VARIOUS FUNCTIONS 10 1 THEORY OF CMOS IMAGE SENSOR The pixel of a CMOS image sensor is composed of the photodiode and the amplifier that converts the charge into voltage Entered light is converted to charge and converted to voltage in the pixel The voltage of each pixel is output by switching the switch one by one Figure 10 1 The scientific CMOS image sensor used in this camera has an on chip CDS correlated double sampling circuit which plays an important role in achieving low noise In addition CMOS image sensor realizes both low noise and high speed readout simultaneously by a split readout scheme in which the top and the bottom halves of the sensor are readout independently and the data of each horizontal line is read by 2 lines of column amplifier and A D in the top and the bottom in parallel and simultaneously Digital data out Digital data out Figure 10 1 Structure of CMOS image sensor 24 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 The exposure and the readout method of CMOS image sensor which this camera adopts is rolling shutter In the rolling shutter the exposure and readout are done line by
5. The unauthorized reproduction or distribution of parts or all of this manual is prohibited e f one of the following problems occurs please contact Hamamatsu Photonics See the CONTACT INFORMATION We will deal with the problem immediately e Some contents of the manual are dubious incorrect or missing Some pages of the manual are missing or in the wrong order e The manual is missing or dirty HAMAMATSU 69
6. External trigger mode Edge trigger 112 Vn 22048 1 int Vn 2048 30 1H x1H 8 104 1 Vn 2x1H 1Hx10 Exp1 External trigger mode Level trigger 112 Vn 2048 1 int Vn 2048 30 1H x1H 85 2104 1 Vn 2x1H 1Hx4 Exp2 External trigger mode Eu trigger 1 int Vn 2048 30 1H x1H readou 1 Vn 2x1H 1Hx18 28 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 Value of frame rate unit 1 1 Normal readout 2 2 1 4 4 1024 1536 2048 Free running mode External trigger mode Edge trigger External trigger mode Level trigger External trigger mode Synchronous trigger readout e The calculation formula and frame rate value of Start trigger mode External trigger mode are same as the free running mode About this mode refer to 10 2 5 4 Start trigger mode mmm mm ee eee dnt E eee ee HAMAMATSU 29 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 2 2 At Slow Scan 1 Camera Link USB 3 0 Vn Number of vertical line The center of the set area is the middle of the sensor Exp1 3 021 ms to 105 1H 32 4812 x 10 e The Exp1 value must be to the calculation formula below in units of seconds Calculation formula Value of Frame rate Frame rate Free running mode 1 Vn 2x1H External trigger mode 1 Vn 2x1H Exp1 1H x10 2048 Edge trigger Level trigger External trigger mode 1 Vn 2x1H 1Hx18 2048 Sy
7. The camera has the free running mode which the exposure and readout timing can be set by software command and controlled by an internal microprocessor The free running mode has normal readout mode in which the exposure time is longer than the 1 frame readout time and electrical shutter mode in which the exposure time is shorter than the 1 frame readout time These readout modes are automatically switched depending on the exposure time setting ee EOC E TEC ET ETT ECC CCCTEE EC CCCCCECE EE COCO CET d RI I D CE TEE EEC CCOCCE LCCC CCCEE EEE Ri edu nde I o I EEC OCOCEC nnee eneen EEC CCCCTE EEE I dende er 10 2 4 1 Normal readout mode The normal readout mode is suitable for observation monitoring field of view and focus adjustment and animation because it can operate with full resolution which is faster than the video rate at Camera Link 100 frame s at USB 3 0 30 frame s In addition the exposure time can be extended to collect more signals and increase the signal to noise ratio if the object is dark In the normal readout mode the exposure time is the same or longer than the 1 frame readout time In this mode the frame rate depends on the exposure time and it becomes frame rate 1 exposure time The maximum exposure time is 10 s Internal exposure timing setting 0H 1H 1023H 1024H 2046H 2047H Sensor readout _ L Camera Link data output __ L L USB 3 0 d
8. 0 AC adapter This is the cord to supply a power supply Use the accessory AC adapter 2 Cooling water hose at Water cooling Option It connects the camera and circulating water cooler The insert position of WATER IN OUT on the camera WATER connector is not specified A CAUTION Please see 8 Water cooling for details 16 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 3 Camera Link interface cable 1 Option This is the cable to connect the Camera Link interface connector 1 of the camera and the Camera Link interface connector 1 on the computer 4 Camera Link interface cable 2 Option This is the cable to connect the Camera Link interface connector 2 of the camera and the Camera Link interface connector 2 on the computer Hamamatsu recommends A11255 05 optional Camera Link interface cable for this camera The camera complies with EMC direction with using A11255 05 Camera Link interface cable Be careful that the camera with other interface cable may not fulfill the EMC directive requirements 5 USB 3 0 interface cable Option This is the cable to connect the USB 3 0 interface connector of the camera and the USB 3 0 interface connector on the computer e Hamamatsu recommends A12467 03 optional USB 3 0 interface cable for this camera The camera complies with EMC direction with using A12467 03 Camera Link interface cable Be careful that the camera with other interface cable may not ful
9. 1 5 11 PRECAUTION WHEN USING CMOS IMAGE SENSOR This camera uses scientific CMOS image sensor Careful attention must be paid to the following points when using CMOS image sensor 1 White spot Subjecting CMOS image sensor to extended exposures may cause failure in part of the silicon wafer resulting in white spots Currently this phenomenon is not currently preventable If CMOS image sensor is at a fixed temperature recurrence of the white spot increases proportionally with the exposure time so this can be rectified with dark subtraction Cosmic ray may generate white spot After acquiring an image using a certain exposure time is loaded CMOS image sensor is exposed to darkness for the same amount of time and another image is obtained After this the difference between the images is determined and the data for the dark portion of the original image is nullified 2 Folding distortion A rough edged flicker may be visible when imaging striped patterns lines and similar subject matter 3 Over light Be careful not to input too strong light such as high energy laser A CAUTION into CMOS image sensor because CMOS image sensor may be damaged by over light 50 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 12 MAINTENANCE 12 1 CARE Perform cleaning of this equipment with the dry soft cloth Then the glass window the image sensor should be cleaned according to
10. C11440 22CUO01 can be attached to F mount lens or an optics system e The depth of the C mount is 7 mm Screwing in the mount too far can scratch the glass surface mmm m mimi mi tdt en Gs nnt B mnt eee eee 2 WATER connector WATER at Water cooling It connects the camera and the circulating water cooler with the cooling water hoses The insert position of WATER IN OUT is not specified A CAUTION Please see 8 Water cooling for details 3 Camera Link interface connector DIGITAL OUT 1 This is connected to the Camera Link interface connector 1 on the computer 4 Camera Link interface connector DIGITAL OUT 2 This is connected to the Camera Link interface connector 2 on the computer 5 Trigger input connector EXT TRIG This is used when the camera is being operated using external synchronization Input is V LVCMOS level and input impedance is 10 When an external trigger is input the trigger is activated at the falling or rising edge of the signal You can choose external trigger polarity between Negative and Positive 6 Timing out connector 1 2 3 TIMING 1 2 3 This is used when peripheral device s require synchronization with the camera Output is 3 3 V LVCMOS level and it is output though BUS TRANSCEIVER IC SN74AVC8T245 Output impedance is 33 7 STATUS lamp STATUS The LED indicates status of camera Turn color Power off G
11. Port F6 Port F7 Port GO Port G1 Port G2 Port G3 Port G4 LVAL D1_10 11 D1 12 D1 13 D1 14 D1 15 MSB D2 0 D2 1 D2 2 D2 3 02_4 02_5 02_6 02_7 02_8 02_9 02_10 D2 11 02 12 02 13 02 14 02 15 MSB 0 03 1 03 2 03 3 03 4 Port G5 Port G6 Port G7 Port HO Port H1 Port 2 Port H3 Port H4 Port H5 Port H6 Port H7 Port 10 Port 11 Port 12 Port 13 Port 14 Port 15 Port I6 Port 17 Port JO Port J1 Port J2 Port J3 Port J4 Port J5 Port J6 Port J7 LVAL 03 5 03 6 03 7 03 8 D3 9 D3 10 D3 11 D3 12 D3 13 D3 14 D3 15 MSB 04 0 D4 1 D4 2 D4 3 D4 4 D4 5 D4 6 04 7 D4 8 D4 9 D4 10 D4 11 4 12 4 13 04 14 D4 15 MSB LVAL Pixel Clock G H l J Pixel Clock ABC PCIk TxCLKln Pixel Clock D E F This signal show the period during which the line part of the image data from CMOS image sensor is in effect This is ON when during the period the line is active LVAL Line Valid signal FVAL Frame Valid signal This signal shows the period during which the vertical part of the image data from CMOS image sensor is in effect This is ON during the period the frame is active This is the image signal data from CMOS image sensor converted A D 00 to D4 has 16 bit data in each MSB shows the most significant bit Please see 14 4 2 OUTPUT TIMING SPECIFICATI
12. which is longer than 10 or not 1H to 1H x Vn 1H x Vn lt 10 s 1H to 10 s 1H x Vn 210 s HAMAMATSU 45 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 3 4 TIMING DIAGRAM Free running mode Internal exposure timing setting l l l N l Sensor readout _____ 3 t Camera Link data output Figure 10 20 Free running mode Edge trigger mode Externaltrigger Sensor readout Camera Link data output Trigger ready output Camera Link Delay 1H S Delaytime is changed by setting of 1H Jitter 1H Figure 10 21 Ex rising edge 46 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 Start trigger mode External trigger LE OH IH 1023H 1024H 2046H 2047H Sensor readout Camera Link data output Trigger ready output Camera Link Delay 1H 9 Delaytime is changed by settingof1H Jitter 1H Figure 10 22 Ex rising edge HAMAMATSU 47 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 3 5 PROGRAMMABLE TIMING OUTPUT By using the programmable timing output synchronizing with external devices is simple A system which needs simple timing signal does not require a delay unit or pulse generator It is possible to program and output a pulse that has an optional pulse width and an optional delay time to the end of readout timing Vsync or Hsync The range of delay is 0 us to 10 5 and range of pulse wid
13. 1x1 2 2 4 4 Digital binning Configurable for each vertical 8 pixels and horizontal 512 pixels 20 ms to 204 8 5 9 7 us to 105 Full Sub array Upper and lower symmetric area 0 06 electron pixel s 0 02 electron pixel s 0 006 electron pixel s 16 bit Less than 3 1 ms to 10 38 96 us to 10 s 1 ms to 105 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 eme ve Free running mode 3 ms to 10 Exposure time Free running mode Slow scan Sub array mode 129 99 us to 10 s External trigger mode 3 ms to 10 s Conversion factor 0 46 electrons count typ Dark offset 100 counts at Normal readout Edge trigger Level trigger Synchronous readout trigger Start trigger External trigger Function External input External signal input SMA connector Camera Link External trigger input level 3 3 V LVCMOS level External trigger output level 3 3 V LVCMOS level External trigger delay function us to 10 10 us steps Global exposure timing output Trigger ready output Programmable timing output 1 Programmable timing output 2 Programmable timing output 3 Continuous High or Low output External input SMA connector External signal output Real time offset correction Image processing function Real time gain correction Real time defect pixel correction Default Camera Link Full configuration Lens mount C mount C11440 22CU F mount C11440 22CUO1 I
14. a Peltier element With a Peltier element when current is supplied one surface is cooled and the other surface is heated CMOS image sensor is positioned on the cooled side and cooling is done by discharging the heat from the heated surface The camera has two cooling modes Air cooling mode and Water cooling mode Cooling mode can be changed by software which is called DCAM Configurator After cooling mode was changed the camera memorizes the last setting as the default setting for cooling The present cooling mode set up of this camera can be checked using DCAM Configurator Air cooling mode The heated side of a peltier element is cooled by a fan inside the Forced air cooled camera When the camera is turned on the fan starts rotating and Default cooling is started Circulating water cooler Optional is used for cooling the heated side of a peltier element Cooling does not start just turning on the camera Cooling water circulation must be started before start operating the camera in water cooling A fan inside the camera does not rotate Please refer to 8 WATER COOLING for instruction of water cooling Water cooling mode 2 Ambient temperature The recommended ambient temperature for camera operation is 20 C Both water cooling or air cooling are available as cooling method CMOS image sensor cooling temperature is more stable under water cooling operation 3 Protection circuit A double prot
15. long exposure time as possible In the synchronous readout trigger mode the camera ends each exposure starts the readout and also at the same time starts the next exposure at the edge of the input trigger signal rising falling edge That is the interval between the same edges of the input trigger becomes the exposure time Sensor readout Camera Link data output 1 i i 1 i l i USB 3 0 data output I l I Global exposuretiming output l 1 Trigger ready output Camera Link i Trigger ready output USB 3 0 Delay 165 58 us Jitter 29 74 Synchronous readout mode and Standard scan mode Delay 552 16 us Jitter 32 48 us Synchronous readout mode and Slow scan mode Delay 38 396 us Jitter 9 74 Fast synchronous readout mode and Standard Scan mode Delay 129 92 us Jitter 32 48 us Fast synchronousreadout mode and Slow scan mode Figure 10 9 Ex rising edge 36 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 2 Pulse count Also in the synchronous readout trigger mode synchronous readout can be controlled by specifying set by command the number of timing pulses to determine the exposure time The following figure shows the exposure timing when the pulse count is set as 3 External trigger Sensor readout Camera Link data output USB 3 0 data output Global
16. to keep clearance least 2 cm from both intake and exhaust vents when setting up 1 2 Screw holes for attaching the option They are used for attaching an adjuster pole A11185 01 or a base plate A1186 01 A11185 01 and A11186 01 are the options of C11440 22C 14 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 7 CONNECTION Place the camera the water connectors to be lateral side Do not place A CAUTION the rear panel of the camera which connectors are located to be at the bottom Do not block ventilation openings Refer to the figure when connecting the various cables Overall connection Camera Rear 2 Cooling water hose 2 hoses 1 AC adaptor Interface cable Computer Interface board Circulating water cooler Figure 7 1 HAMAMATSU 15 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 Connection to Camera Link Connection to USB 3 0 Camera Rear Camera Rear OOS EN CD AC adaptor 3 Camera Link Camera Link interface cable 1 interface cable 2 interface cable Computer Computer To connector 2 e When a connection interface is changed from Camera Link to USB 3 0 and vice versa the application software must be closed and the camera must be turned off
17. 0 Less than 100 ms There is invalid readout of 1 frame More than 100 ms There is Delay of 100 ms Figure 10 11 Ex rising edge 10 2 5 5 External trigger delay function In most cases when a delay is needed between the laser pulse emission and the exposure start is needed a delay unit is set between the laser and camera to control trigger timing In each external trigger mode of the camera the delay can be set to the trigger signal input to the camera by command With this setting a range of trigger can be arranged without a delay unit The range for delay time is O us to 10 s 10 us steps 38 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 6 TRIGGER OUTPUT The camera provides a range of trigger output signals to synchronize with an external instrument and the camera becomes the master and the external instrument becomes the slave There are three different trigger output functions as follows Also it can output continuous High output High output fixed or continuous Low output Low output fixed These three different trigger output functions can be selected by software command and they are output from Timing out connector 10 2 6 1 Global exposure timing output shows the global exposure timing where all lines expose at the same time There is a case that one event is divided into two frames because the timing of the exposure in each line is different for the rolling shutter However by u
18. 0 22CU C11440 22CU01 Instruction manual Ver 1 5 13 TROUBLESHOOTING CHECKLIST If an abnormality occurs look up the possible causes in the following tables and if necessary report the details to Hamamatsu subsidiary or local distributor 13 1 IMAGE IS NOT TRANSFERRED Cs chapter AC adapter or other cable is loose Reconnect the cable AC adapter or other cable is broken Replace the cable The correct command has not been sent to the camera Recheck command 13 2 ALTHOUGH IMAGES ARE TRANSFFERED 1 Scratches or discoloration visible on the screen Lens is dirty Wipe the lens 12 2 Image is blurred Chapter Lens is not focused Contact Hamamatsu subsidiary or local distributor Condensation appear Confirm the operating environmental conditions 3 Only shadowed images are output i Measures Chapter Lens mount cap has been left on Remove the cap Amount of light is too much or too low Reduce amount of light 4 All screens overflow Cause o Meaues 3 Chapter Too much amount of light Reduce amount of light Contrast enhancement is too high Reduce gain 5 Noise appears on the screen Exogenous noise Find and remove cause Poor connection of internal connector Contact Hamamatsu subsidiary or Defective circuit system local distributor HAMAMATSU 53 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 14 SPECIFICATIONS 14 1 CAMERA SPEC
19. IFICATIONS 1 Electric specifications Camera veen Imaging device Effective number of pixels Cell size Effective area Full well capacity typ Readout noise typ Dynamic range Cooling method Cooling temperature Frame rate Top standard scan Bottom slow scan Readout mode Light sheet mode Dark current A D bit Linearity Exposure time Standard scan 54 at Forced air cooled at Water cooled at Maximum cooling at Full resolution at 1024 lines at center position at 8 lines at center position at Horizontal 512 pixels at 8 lines at center position Normal readout mode Binning readout mode Sub array readout mode Readout time Exposure time Readout mode at 10 at 20 C at 30 C Free running mode Free running mode Sub array mode External trigger mode Scientific CMOS image sensor 2048 H x 2048 V 6 5 um H x 6 5 um V 13 312 mm H x 13 312 mm V 30 000 electrons 1 0 electrons median 1 6 electrons r m s at standard scan 0 8 electrons median 1 4 electrons r m s at slow scan 37 000 1 Peltier device Forced air cooled Water cooled 10 C Ambient temperature 20 C 20 C Water temperature 20 C 30 typ at Water cooled Water temp 15 C Ambient temp 20 C 100 frame s 30 frame s 200 frame s 60 frame s 25 655 frame s 7 696 frame s 30 frame s 60 frame s 7894 frame s 25 655 frame s
20. ONS for details 0 to D4 15 Digital image data HAMAMATSU 61 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 14 4 2 OUTPUT TIMING SPECIFICATIONS 1 Normal readout Camera Link Timing Normal C L Fvalid C L Lvalid C L Fvalid C L Lvalid V1023 V1024 V1022 V2 V1 VO Tx Clock 85 MHz CL Fvalid 410 clock 4 clock V1024 cen en cn U Upper bte T rev oo eee peso ee T Tee Tn Dr Dee ee e a peo De Deeper Te T een Des Dee Tee er e empero Tes Te Tee or Dev Des Tee ee e Fem D Dee Te T Te Tee Dee Tee ee e Eee er CL Lvalid CL Port A Data CL Port B Data CL Port C Data CL Port D Data CL Port E Data CL Port F Data CL Port G Data CL Port H Data CL Port Data CL Port J Data 62 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 2 2x2 binning readout Camera Link Timing 2x2 Binning C L Fvalid C L Lvalid C L Fvalid C L Lvalid 511 512 510 V2 V1 Vo Tx Clock 85 MHz CL Fvalid 205 clock 209 clock 512 EN rn ea ar mr E U Upper byte vu wu wou vee mo Teese D De Leelee Dee Te Tee Pen edelen oe Tee Te a Dev Te er Teen Dec De elfde Tee wou rau D ot ame Te Joo
21. ORCA Flash4 0 V2 Digital CMOS Camera C11440 22CU C11440 22CU01 Instruction manual Thank you for your purchase e Follow the safety precautions in Chapter 1 in order to avoid personal injury and damage to property when using this camera Be sure to read CAUTION this Instruction manual beforehand in order to use the digital camera correctly The manual describes the correct method of handing the camera and provides cautions in avoid accidents e After reading keep the manual where it can be referred to at any time Ver 1 5 Sep 2015 HAMAMATSU PHOTONICS 95900306 05 Blank page C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 1 SAFETY PRECAUTIONS 1 1 INDICATION OF THE SYMBOLS The following symbols can be found on this camera Direct current Alternating current 1 2 CLASSIFICATION OF WARNING We have classified the warnings symbols that appear in this instruction manual and on the camera as follows for your convenience Make sure that you fully understand them and obey the instructions they contain Improper handling of the camera without observing these warnings could lead to serious injury to the user and even death Improper handling of the camera without observing these cautions CAUTION could lead to personal injury to the user or damage to property This symbol indicates a note to help you get the best performance from the camera Read the contents of the note carefully
22. ata output Global exposure timing output 10 4 32 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 4 2 Electrical shutter mode The electrical shutter mode is used to get a proper signal level when signal overflow happens due to too much input photons in normal readout mode In this mode the fastest frame rate is 100 Hz via Camera Link or 30 Hz via USB 3 0 at full resolution even when the exposure time is short Camera Link Internal exposure time setting 0H 1H 1023H 1024H Exposure 2046H 2047H Sensor readout Camera Link data output Global exposure timing output USB 3 0 Internal exposure time setting Exposure Sensor readout USB 3 0 data output Global exposure timing output m Figure 10 6 HAMAMATSU 33 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 5 EXTERNAL TRIGGER MODE The camera has various external trigger functions to synchronize the camera with the external equipment In the external trigger mode the external equipment becomes a master and the camera becomes a slave 10 2 5 1 Edge trigger mode The edge trigger mode is used so that the exposure starts according to an external signal Exposure time is set by software command In this mode the exposure of the first line begins on the edge rising falling timing of the input trigger signal into the cam
23. ation At the Water cooling if ambient temperature and ambient humidity become high condensation will take place easily Use the camera under the environment where condensation will not take place referring to the following graph Relations between ambient temperature and ambient humidity Water cooling water temp 20 Maximum cooling water temp 15 C Ambient humidity 10 15 20 25 30 39 40 Ambient temperature C 4 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 2 CHECK THE CONTENTS OF PACKAGE When you open the package check that the following items are included before use If the contents are incorrect insufficient or damaged in any way contact your local dealer without attempting to operate the camera Camera C11440 22CU or C11440 22CU01 AC adapter Power supply cord for AC adapter Lens mount cap attached to the camera C11440 22CU C11440 22CU01 Before Use Booklet C11440 22CU C11440 22CUO01 Instruction manual CD ROM QC sheet Option Cooling water hose 2 hoses A10788 04 SMA BNC cable A12106 05 SMA SMA cable A12107 05 Camera Link interface cable A11255 05 USB 3 0 interface cable 12467 03 USB 3 0 interface board M9982 21 Adjuster pole for C11440 22C A11185 01 Base plate for C11440 22C A11186 01 Camera Link interface board M9982 20 e The cable listed in option is highly recommended for use with the camera The camera system may not con
24. ccording to the table below Settings Interface Position Position 1 1 Normal readout 512 pixels 32 pixels 8 lines 4 lines 2x2 binning readout 256 pixels 16 pixels 4 lines 2 lines 4x4 binning readout 128 pixels 8 pixels 2 lines 1 lines Please refer to 10 2 22 2 FRAME RATE CALCULATION about the frame rate of each 26 readout mode HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 2 FRAME RATE CALCULATION 10 2 2 1 At Standard Scan 1 Camera Link Vn Number of vertical line The center of the set area is the middle of the sensor Exp1 1 004msto10s 1H 9 74436 x 10 Calculation formula Value of Frame rate Calculation formula Frame rate frame s Free running mode 1 Vn 2x1H External trigger mode 1 Vn 2x1H Exp1 1H x10 2048 Edge trigger Level trigger External trigger mode 1 Vn 2x1H 1Hx18 2048 Synchronous trigger readout External trigger mode 1 Vn 2x1H 1Hx5 2048 Fast Synchronous trigger readout HAMAMATSU 27 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 2 USB 3 0 Vn Number of vertical line The center of the set area is the middle of the sensor Exp1 1 004msto10s Exp2 1 05msto10s 1H 9 74436 x 10 int The decimal point is rounded down e The Exp1 and Exp2 values must be input to the calculation formula below in units of seconds Free running mode 1 int Vn 2048 30 1H x 1H 1 int Vn 2048 100 1H x1H
25. e trigger mode the next frame can start after the previous frame exposure is done Thus the camera cannot accept a trigger for the next frame during the exposure period To reduce useless time to be as short as possible it is necessary to know the period when the camera can accept a trigger for the next frame The trigger ready output shows the trigger ready period when the camera can accept an external trigger in the external trigger mode 40 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 7 SLOW SCAN can change from the standard scan Default to the slow scan with a command Read out noise is reduced with slow scan Please refer to Figure 10 2 2 FRAME RATE CALCULATION about the frame rate value and calculation formula with slow scan 1 1 F t nn E 10 2 7 1 Timing Diagram The read out time is 33 ms at full frame and the frame rate is 30 frames s at fastest The timing diagram for slow scan is similar to the timing diagram for the standard scan except readout time Please refer to the timing diagram of standard scan for each trigger mode Internal exposure timing setting DH 1H 1023H 1024H 2046H 2047H Readout time Sensor readout SI gt Camera Link data output Global exposure timing
26. ection circuit protects this camera s thermoelectric cooling device If the heat dissipater becomes abnormally hot the protection circuit sets off a buzzer alarm and stopping current supply to Peltier element simultaneously When the protection circuit is activated immediately turn off the power switch Then investigate cause and remove the cause of the overheating and restart the camera 22 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 9 2 PREPARATION FOR IMAGING Use the following procedure when start operating the camera pe nnen LE EP EE e Please change cooling method cooling mode if needed The present cooling mode set up of this camera can be checked using DCAM Configurator 9 2 1 WHEN USING AIR COOLING 1 Connect the equipment as shown in Figure 7 1 before operating of the camera 2 Turn camera 3 Check cooling fan is operating properly and air is circulating 9 2 2 WHEN USING WATER COOLING 1 Connect the equipment as shown in Figure 7 1 before operating of the camera 2 Turn camera 3 Turn on the circulating water cooler 4 Check cooling water is circulating suitably 5 Turn on the cooling switch of the camera from application software Please refer to the manual of application software for ON OFF of the cooling switch of a camera 9 3 END OF IMAGING Follow the procedure below when imaging is finished
27. era 1023H and 1024H in the following figure The exposure of the second line is begun after the readout time of one line passes 1022H and 1025H in the following figure and the exposure is begun one by one for each line 1 1 1023H 1024H 2046H 2047H L USE 3 0 data output i Global exposure timing output Trigger ready output Camera Link Trigger ready output USB 3 0 Delay 87 7 us litter 9 74 us Delay 292 33 us litter 32 48 us Slow scan mode Figure 10 7 Ex rising edge 34 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 5 2 Leveltrigger mode The level trigger mode is used to control both exposure start timing and exposure time length by inputting external trigger pulses In the mode the camera starts exposure at the start of high or low period of the input trigger pulse and stops exposure at the end of high or low period of the input trigger pulse The example below is for the trigger level High The exposure of the first line begins when the trigger signal becomes High and the exposure of the second line begins after the readout time of line one passes Each exposure begins one by one for each line The exposure of the first line is finished when the trigger signal becomes low and signal readout is begun The exposure time of each line is defined by the time that the input trigger is high The minimum trigger pulse width i
28. exposure timing output Trigger ready output Camera Link Trigger ready output USB 3 0 Delay 165 58 us Jitter 9 74us Synchronous readout mode and Standard scan mode Delay 552 16 us litter 32 48 us Synchronous readout mode and Slow scan mode Delay 38 96 us Jitter 9 74 us Fast synchronous readout mode and Standard Scan mode Delay 129 92 ps litter 32 48 ps Fast synchronous readout mode and Slow scan mode Figure 10 10 Pulse count HAMAMATSU 37 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 5 4 Start trigger mode The start trigger mode is to start operating the camera by a trigger input for a continuous imaging It is useful to secure the frame rate as fast as possible when continuous image acquisition and not to sacrifice the exposure time For example when it is necessary to measure the phenomenon after stimulation it is possible to start continuous image acquisition at the stimulation timing The start trigger mode is to start operating the camera by a trigger input for continuous imaging and it works at the highest frame rate because it is operated in internal trigger mode In the start trigger mode the camera starts exposure and switches to internal trigger mode by the edge of an external trigger signal rising falling edge 1024H 2046H 2047H Global exposure timing output Trigger ready output Camera Link Trigger ready output USB 3
29. fill the EMC directive requirements HAMAMATSU 17 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 8 WATER COOLING e Improper handling of the camera without observing these cautions NCAUTION s 9 9 could lead to personal injury to the user damage to property 8 1 1 2 3 CAUTIONS Cooling water It is recommended to use soft water except deionized water for cooling water If you plan on using water other than soft water as recommended for example antifreeze etc please refer to description of cooling water which is written in 12 Maintenance or contact Hamamatsu subsidiary or local distributor Recommendation ambient temperature Hamamatsu recommends 20 for Circulating water temperature For the appropriate temperature range of the cooling water confirm with the instruction manual of your circulating water cooler Condensation Use the camera under the environment where condensation will not take place referring to the following graph Relations between ambient temperature and ambient humidity Maximum cooling water temp 15 Water cooling water temp 20 C 70 60 50 x 40 5 5 30 4 2 lt 20 10 0 15 20 25 30 35 40 Ambient temperature HAMAMATSU 4 5 6 7 8 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 Handling of the circ
30. firm to CE marking regulation if other type of cable is used with e The cooling is set as air cooling at factory setting It is possible to change the cooling setting from Air cooling to Water cooling by the special software included e Handle the circulating water cooler and the cooling water according to an instruction manual of the circulating water cooler HAMAMATSU 5 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 3 INSTALLATION Avoid using or storing this camera in the following places When ambient temperature for using this system might fall below 0 C or rise S 40 e When the ambient temperature for storing this system might fall below 10 C or above 50 e Where the temperature varies extremely e In direct sunlight or near a heater e Where the humidity is 70 or more or where there is dripping water Close to a strong source of magnetism or ratio waves e Where there is vibration e Where it might come into contact with corrosive gases such as chlorine or fluorine e Where there is a lot of dust Do not place the camera the rear panel of the camera which connectors are located to at the bottom Do not block ventilation openings o oo r A A r P DT O 4 2 in cloth or other material or in any way allow the camera s ventilation ports to become blocked If the camera is be
31. g shocks by dropping it for example Doing so can damage the camera At the water cooling Be careful water does not splash on the camera Cut off the power supply of the circulating water cooler and the camera when you remove and install the cooling water hoses Operating environment This system is designed and tested for use in an industrial environment If this system is used in residential areas EMI electro magnetic interference may occur This system must not be used in residential areas eooe e AE HAMAMATSU 3 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 ACAUTION Disposal When disposing of the camera take appropriate measures in compliance with applicable regulations regarding waste disposal and correctly dispose of it yourself or entrust disposal to a licensed industrial waste disposal company In any case be sure to comply with the regulations in your country state region or province to ensure the camera is disposed of legally and correctly Cooling water is recommended to use soft water except deionized water for cooling water Follow instruction manual which is attached to your circulating water cooler for an appropriate temperature range of cooling water If you plan on using water other than soft water as recommended for example antifreeze etc please refer to description of cooling water which is written in 12 Maintenance or contact Hamamatsu subsidiary or local distributor Condens
32. h 2048 pixels x 2048 pixels with very low readout noise which was impossible can now be achieved 2 Cooling structure In the camera CMOS image sensor is cooled down by the peltier element to suppress the dark current If CMOS image sensor is exposed to the atmosphere condensation of the moisture from the air might occur However the camera has a special hermetic chamber structure to isolate the sensor from the atmosphere and the chamber is filled with nitrogen gas 3 Pixel number and pixel size CMOS image sensor has 6 5 um x 6 5 pixel sizes that is equivalent to conventional CCD image sensor 2 3 inch 1 3 megapixels Also the camera can observe a wider field of view because the pixel number is about 3 times that of the conventional CCD image sensor 2 3 inch 1 3 megapixels 4 Readout method The camera has a variety of readout modes In addition to full resolution readout mode sub array readout and binning readout are supported HAMAMATSU co C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 5 6 7 Frame rate readout speed CMOS image sensor which this camera adopts realizes both low noise 1 0 electrons median 1 6 electrons r m s and high speed readout 100 frame s with 2048 pixels x 2048 pixels simultaneously by a split readout scheme in which the top and the bottom halves of the sensor are readout independently and the data of each horizontal line is read by 2 lines of column am
33. image with moderate transfer speed FESS ee es ne thet he ne ueteres ems s esi mue eu suus mu eum neue ym eum eum nn be nn ne nnn nen nn When a connection interface is changed from Camera Link to USB 3 0 and vice versa the application software must be closed and the camera must be turned off 8 Camera readout modes The camera has two scan speed in Normal area mode and two readout direction in Light sheet mode HAMAMATSU 11440 22 0 11440 22 001 Instruction manual Ver 1 9 Camera operation modes The camera has three operation modes 1 the free running mode in which the exposure and readout timing are controlled by the internal microprocessor and 2 the external trigger mode in which the exposure and readout timing are decided by an external trigger 3 the start trigger mode is used to start operating the camera by a trigger input for a continuous imaging 5 HAMAMATSU 11 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 6 NAME AND FUNCTION OF THE PARTS 1 C11440 22CU 2 C11440 22CU01 12 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 e Place the camera the water connectors to be lateral side Do not place A CAUTION the rear panel of the camera which connectors are located to be at the bottom Do not block ventilation openings D Lens mount C11440 22CU can be attached to C mount lens or an optics system
34. ing operated in an enclosed environment ensure clearance of at least 2 cm from both the intake and exhaust vents when setting up SQ To prevent overheating the camera s interior do not wrap the camera Be careful not to drop off the camera or not drop underfoot when making it move because it is approx 2 kg 6 HAMAMATSU eS m eS gt C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 Contents SAFETY PRECAUTIONS vs 1 1 1 INDICATION OF THE SYMBOLS ente tete tette tette 1 1 2 CLASSIFICATION OF WARNING 9 1 CHECK THE CONTENTS OF 5 INSTALLATION 6 OVERVIEW 9 FEATURES ee 9 NAME AND FUNCTION OF THE 5 12 CONNECTION 15 WATER COOLING 18 8 1 CAUTIONS neee ennen ennen ananas 18 8 2 CONNECTION OF WATER COOLING HOSES een 20 8 3 DISCONNECTION OF WATER COOLING 21 OPERATION es 22 9 1 PRECAUTIONS eccccececececesecececscscecececeveveveveveusesesesesesesesessesssssssssecsteessscssevevevevevenes 22 9 2 PREPARATION FOR IMAGING nanne 23 9 2 1 WHEN USING AIR COOLING ennennnerennennnen ener ensen serene vennen eneen sense 23 9 2 2 WHEN USING WATER COOLING vt 23 9 3 END OF IMAGING 23 DESCRIPTION OF VARIOUS FUNCTIONS anne
35. ing water cooler Remove the hose on circulating water cooler side Follow the instruction on the circulating water cooler when you disconnect the hose from the circulating water cooler Remove water or water drop inside the hose and the camera by air Blow Air from one side of hose Prepare water absorption sheet such as Waste Towel or so and catch pan on another side of hose in order to avoid water drop or water splash Blow Air until no water drop come out Remove the camera from the microscope if the camera is used on the microscope It is not necessary to remove the camera from the microscope if it is possible to remove the hoses from the camera as it is Place the camera on the stable table Put the lens cap on to protect the sensor Change the WATER connector direction to be downward Prepare water absorption sheet such as Waste Towel or so and catch pan Remove hoses one by one and wipe water Disconnect hoses with pushing button while being careful not to splash water Figure 8 4 e Cooling water may be left inside the camera even after hoses are removed AC AUTION In such case remove water inside by blowing air from connectors Be careful not to splash water onto the camera HAMAMATSU 21 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 9 OPERATION 9 1 PRECAUTIONS Be careful of the following when you operate the camera 1 Cooling method Cooling of this equipment is done using
36. ion of the cooling water hose Replace the water hose with a new one whenever it cannot keep the Keep 1 0 L min flow rate for water circulation due to the hose deterioration HAMAMATSU 19 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 20 1 2 3 4 5 CONNECTION OF WATER COOLING HOSES Water cooling hose WATER connector Figure 8 3 Place the camera on the stable table Connect water cooling hose into the WATER connector on the camera e Insert the hose fully into the WATER connector on the camera as shown in Figure 8 3 e Confirm the hose stops at it Set the camera onto a microscope If the camera is used on the microscope If it is easy to connect the hose onto the camera after the camera is set onto the microscope then it is OK to connect the hose after the camera is set on the microscope Connect the hose onto the circulating water cooler Follow the instruction on the circulating water cooler when you connect the hose onto the circulating water cooler Turn on the circulating water cooler and confirm the cooling water is flowing normally e Stop the circulating water cooler when the water flow is abnormal CAUTION or water drop or splash is found HAMAMATSU 1 2 3 4 5 6 7 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 DISCONNECTION OF WATER COOLING HOSES Turn camera power and all peripheral devices including circulat
37. line Therefore the exposure timing is different on one screen Figure 10 2 But even if the object moves during the exposure the affect of rolling shutter is very small Rolling shutter Exposure length is same for all lines Exposure timing is different for all lines Figure 10 2 Readout timing of Rolling shutter HAMAMATSU 25 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 NORMAL AREA MODE 10 2 1 READOUT METHOD SCAN MODE 1 2 3 4 Readout direction The camera readout the image sensor from the center line to the top and from the center line to the bottom simultaneously center line is depicted in red line in the diagram Figure 10 3 Normal area mode readout direction Normal readout Perform charge readout from camera individually for all pixels Binning readout With this camera 2 2 binning readout and 4 4 binning are available by adding the signal of adjacent pixels in the digital domain Binning readout is a method for achieving high sensitivity in exchange for losing resolution Sub array readout Sub array readout is a procedure only a region of interest is scanned It is possible to increase the frame rate by reducing the number of vertical lines scanned When a target area is placed in the center of the screen sub array readout can perform the fastest readout In sub array readout binning configuration is enabled Size and a position of the readout area can be configured a
38. m s readout noise performance Moreover the camera delivers high sensitivity through its on chip micro lens 37 000 1 high dynamic range that make the camera suitable for almost any scientific application from bright field imaging to low light fluorescence imaging across a wide spectral range Various external trigger functions and timing output functions ensure proper timing control with peripheral equipment to cover a wide range of applications The camera is the new scientific digital camera for life science microscopy semiconductor inspection x ray scintillator readout or industrial imaging 5 FEATURES 1 Readout noise In the camera the pixel amplifier is optimized it has high gain from optimizing the semiconductor process and the difference among pixel amplifiers are greatly minimized In addition there is on chip CDS correlated double sampling circuit which plays an important role in achieving low noise Moreover the sensor features a split readout scheme in which the top and bottom halves of the sensor are readout independently and the data of each horizontal line is read by 2 lines of column amplifier and A D in the top and the bottom in parallel and simultaneously As a result it achieves very fast readout speed while keeping very good low noise performance The camera has lower readout noise 1 0 electrons median 1 6 electrons r m s than the conventional cooled CCD camera Moreover high speed readout 100 frame s wit
39. n 24 10 1 THEORY OF CMOS IMAGE SENSOR eet tette 24 10 2 NORMAL AREA MODE nnee 26 10 21 READOUT METHOD SCAN MODE nenn 26 10 2 2 FRAME RATE CALCULATION 27 10 2 3 CONFIGURING EXPOSURE TIME IRR IR RRRIRRRIRIRRRRRI RR RR 31 10 2 4 FREE RUNNING 32 10 2 5 EXTERNAL TRIGGER MODE aa 34 10 2 6 TRIGGER OUTPUT 39 1022 7 SLOW SCAN a ete e E n 41 10 2 8 GLOBAL RESET En nn nun na EEE nn nn En RU EU Ran aaa paar una 42 10 3 LIGHT SHEET MODE e e reer r areae r renmenm enaena ennea a nannan akoako n knakar nean 44 10 3 1 READ OUT DIRECTION ss set rie vn telen 44 10 3 2 FRAME RATE CALCULATION cccccccccecccccccecceueccecutensceueatenucecscuneatenuunsaesutenses 45 10 3 3 READ OUT TIME OF THE HORIZONTAL LINE 45 10 3 4 TIMING DIAGRAM ss 46 10 3 5 PROGRAMMABLE TIMING OUTPUT 48 10 4 REAL TIME CORRECTION FUNCTIONS Re 49 PRECAUTION WHEN USING CMOS IMAGE 50 NANN O 51 12 1 51 HAMAMATSU 7 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 13 14 15 16 17 12 2 INFORMATION ON COOLING WATER FOR THE CIRCULATING WATER COOLER M
40. nchronous trigger readout External trigger mode 1 Vn 2x1H 1Hx5 2048 Fast Synchronous trigger readout Camera Link 30 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 3 CONFIGURING EXPOSURE TIME The exposure time setting can be done by absolute value The actual exposure time setting is defined by the following formula and the camera automatically calculates a larger and closest value from the specified exposure time setting 1 Standard scan Exp1 1 ms to 10 s at sub array 38 96 us to 10 s 2 Slow scan Exp1 3 ms to 10 s at sub array 129 99 us to 10 s 2 Exp1 x 10 32 4812 us round up at decimal point tr 1 32 4812 us x Exp2 Available setting range of the exposure time is the following Standard scan Free running mode 1 ms to 10 s ms to 10 s Free running mode at Sub array 38 96 to 10 s 129 99 us to 10 s External trigger mode 1 ms to 10s ms to 10 s 38 96 Standard scan and 129 99 ys Slow scan is the minimum exposure time when sub array is set to 8 lines vertically symmetric 4 lines in top half and 4 lines in bottom half with respect to the horizontally center axis The minimum exposure time vary depend on vertical line number of sub array setting HAMAMATSU 31 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 4 FREE RUNNING MODE
41. ness or forming foreign substances It cause lower flow rate or water flow stop Distilled water Deionized water When using the camera inside clean room it is possible to use distilled water or deionized water by conducting periodical check However please notice it increases possibility of corrosion inside cooling water path lowering flow rate or water flow stop e Monthly check Check water impurity non existence of sliminess foreign particle is not mixed with water or not adhered inside water path and no unusual odor If you find any of the issues please exchange cooling water and clean cooling water path Soft water from tap e It is possible to use soft water from tap with conducting periodical change of cooling water and check up However please notice it increases possibility of corrosion inside cooling water path lowering flow rate or water flow stop e Monthly check Check water impurity non existence of sliminess foreign particle is not mixed with water or not adhered inside water path and no unusual odor If you find any of the issues please exchange cooling water and clean cooling water path Exchange cooling water every 3 months e Clean cooling water path every 6 months Bottled water One example of soft water which is commonly available is mineral water Hardness less 92 than 70 Please check hardness of water by referring product information of bottled water manufacturer HAMAMATSU C1144
42. nterface USB 3 0 Super Speed 4 Calculated from the ratio of the full well capacity and the readout noise 2 Digital binning processing in the camera 3 Original mode based on 80 bit mode 2 Power supply specifications Input power supply Camera Typical output Power consumption Input power supply 100 VtoAC240V 50 60 2 5A AC adapter Typical output DC 12V 834A Power consumption 70 VA e Fluctuations of input power supply voltages are not to exceed 10 of the nominal voltage HAMAMATSU 55 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 3 Operating environment Ambient operating temperature 0 C to 40 Ambient storage temperature 10 C to 50 C Ambient operating humidity at Forced air cooled Less than 70 no condensation Less than 50 at ambient temperature 20 C Less than 40 DEN at ambient temperature 20 C Ambient storage humidity Less than 90 no condensation Operating space Indoor altitude up to 2000 m 4 Dimensional outline and weight Dimensional outline 85 mm W x 85 5 mm H x 125 mm Weight Approx 2 0 kg Camera only careful not to drop off the camera or not drop underfoot when making it move because it is approx 2 0 kg 5 Applicable standards EN61326 1 2013 Class 56 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 14 2 CONDENSATION At Water cooling if ambient temperatu
43. output Figure 10 13 HAMAMATSU 41 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 8 GLOBAL RESET 10 2 8 1 Timing diagram Global reset function enables to reset the electric charge of all pixels at the same time Then all pixels can start exposure at the same time Global reset can work with Edge trigger mode and Level trigger mode Edge trigger mode External trigger FLO FL Sensor readout Camera Link data output USB 3 0 data output Global exposure timing output Trigger ready output Camera Link Trigger ready output USB 3 0 Delay 87 7 us jitter 9 74 us Delay 292 33 us litter 32 48 us Slow scan model Figure 10 14 42 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 Level trigger mode External Trigger jo L4 L o Sensorreadout Global exposure timing output USB 3 0 data output Trigger ready output Camera Link Trigger ready output USB 3 0 Delay 87 7 us jitter 9 74 5 Delay 292 33 us Jitter 32 48 us Slow scan mode Figure 10 15 HAMAMATSU 43 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 3 LIGHT SHEET MODE 10 3 1 READ OUT DIRECTION With normal area mode the camera readout from the center line to the top line and to the bottom line simultaneously With light sheet mode the camera readout from the top to the bottom line or from the bottom to the top line Light sheet mode works wi
44. plifier and A D in the top and the bottom in parallel and simultaneously Real time correction functions When using the camera there is a case that shading caused by uneven illumination or optics is not negligible in the image Also there are a few pixels in CMOS image sensor that have slightly higher readout noise performance compared to surrounding pixels For those cases the camera has real time offset level shading and defective pixel correction features to further improve image quality The correction is performed in real time without sacrificing the readout speed at all Interface This camera has both Camera Link and USB 3 0 interface Camera Link Interface Camera Link interface is able to transfer large volumes of data It can transfer 4 megapixels image with 100 frame s In order to realize such large volume data transfer the camera uses Camera Link Full Configuration Deca Mode which is an expanded version of Camera Link Full Configuration It enables to transfer maximum 85 MHz x 10 Taps 8 bit image data to computer as fast as 100 frame s In order to use this interface a Camera Link interface board which supports Full Configuration Deca Mode is required m USB 3 0 Interface USB 3 0 interface is able to transfer 4 megapixels image with 30 frame s It is versatile interface which is suitable to use when fast data transfer is not required This interface does not require a Camera Link interface board It transfers
45. re and ambient humidity become high condensation will take place easily Use the camera under the environment where condensation will not take place referring to the following graph Relations between ambient temperature and ambient humidity Water cooling water temp 20 Maximum cooling water temp 15 C Ambient humidity 15 20 25 30 35 40 Ambient temperature Figure 14 1 HAMAMATSU 57 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 14 3 SPECTRAL RESPONSE CHARACTERISTICS TYP 25 o o S 3 Tu 2 Q i 600 700 800 900 Wavelength nm Figure 14 2 58 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 14 4 INTERFACE SPECIFICATIONS 14 4 1 CAMERA LINK INTERFACE The camera is based on Camera Link interface Camera Link full configuration Deca mode that transfers 80 bit data in parallel 8 bit x 10 port It is an extended interface of Camera Link full configuration Standard 1 Pin assignments of Camera Link connector 1 SDR 26 Camera connector Frame grabber connector Channel Link signal HAMAMATSU Inner Shield X0 1 2 Xclk X3 SerTFG CC1 2 CC3 CC4 Inner Shield Inner Shield 0 1 2 Xclk X3 SerTC SerTFG CC1 CC2 CC3 CC4 Inner Shield 59 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 60 2 Pin assignmen
46. reen Blinking Initialization Green lighting Power on Orange lighting Data transfer Red lighting Heat up When the camera heats up stop operation and unplug the AC NCAUTION adapter immediately HAMAMATSU 13 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 Power switch POWER The power is turned on off When the power switch is set to the camera turns on and starts initialization and the lamp blinks in green When the initialization is completed the lamp color stays in green When the camera transfers data and the lamp color is orange When the power switch is set to OFF the camera returns to the power off state and the lamp turns off 9 DC power input connector DC IN This is the power supply terminal Use the accessory AC adapter USB 3 0 interface connector USB 3 0 This is connected to the USB 3 0 interface connector on the computer When a connection interface is changed from Camera Link to USB 3 0 and vice versa the application software must be closed and the camera must be turned off To prevent overheating inside the camera do not wrap the camera in cloth or other material block the camera s ventilation minimi m m i i i i ki i li lik i i i i i lik i i ii i iii i iii i ik i i li i lik i ili i ik it lili ik ilk i lik i ilk i lik i ll it lik i ilk iii lk i lik i i lk kik i i ie e f the camera is being operated in an enclosed environment ensure
47. rs Such as the image suddenly disappearing or a strange noise smell or see smoke coming from the camera stop the power supply immediately and contact Hamamatsu subsidiary or local distributor Never attempt to repair the camera yourself BOM OC 2 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 ACAUTION AC adapter When unplugging the AC adapter always pull by the plug but remove the plug from the outlet to avoid causing electric shock or fire When the system is not used for a long period of time unplug the power supply cord from the outlet to avoid damaging the cord and causing electric shock or fire Connecting and disconnecting cables Always turn off the power supply of the peripheral device before connecting and disconnecting cables Fixed the camera When fitting the camera to a tripod or other fixture use the optional base plate Be careful that the fitting screw does not enter more than 8 mm from the surface of the base plate Screwing it in excessively can impair normal operation Lenses C11440 22CU Be careful not to screw the lens more than 7 mm onto the C mount of the camera Doing so can scratch the protective glass Some wide angle lenses in particular can have a thread of 7 mm or more Shipping precautions When transporting the camera by truck ship airplane etc wrap it securely in packaging material or something similar Strong impact Do not subject the camera to stron
48. s 1 ms 50 us Externaltrigger gt en Trigger ready output Camera Link Trigger ready output USB 3 0 Delay 87 7 us litter 9 74 us Delay 292 33 us Jitter 32 48 us Slow scan mode Figure 10 8 Ex rising edge HAMAMATSU 35 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 2 5 3 Synchronous readout trigger mode The synchronous readout trigger mode is used for continuous imaging when it is necessary to control the exposure start timing of each frame from an external source It is useful for confocal microscopy For example when the camera is used with a spinning disk confocal microscope and the camera exposure time is synchronized to the spinning disk s rotation speed it is possible to eliminate uneven illumination called banding noise caused by variation of the spinning disk rotation speed Also it is useful for securing as long exposure time as possible while controlling the exposure start timings by external trigger signals It is possible to work with shorter interval time if you set fast synchronous readout trigger mode Fast synchronous readout trigger mode works with only Camera Link interface operation 1 Normal operation when the pulse count is set as 1 The synchronous readout trigger mode is used for continuous imaging when it is necessary to control the exposure start timing of each frame from an outside source and also when it is necessary to secure as
49. sing the Global exposure timing output the global exposure becomes possible for the phenomenon that happens for this period Global exposure timing output shows the period where all lines expose at the same time 10 2 6 2 Programmable timing output By using the programmable timing output synchronizing external devices is simple A system that needs simple timing signal does not require a delay unit or pulse generator It is possible to program and output a pulse that has an optional pulse width and an optional delay time to the end of readout timing or Vsync The setting range for delay time is 0 us to 10 s and the setting range for pulse width is 1 us to 10 The relation between the parameter which can be set with each reference signal and an output signal becomes below signal Camera outputs a pulse after certain delay from the end of sensor readout Also the Read End pulse width can be set V Camera outputs a pulse after certain delay from the beginning of readout Also the Sync i pulse width can be set HAMAMATSU 39 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 External Trigger T LL Sensor readout Read End fee Delay Pulse Width Vsync ke Delay Pulse Width Figure 10 12 Programmable timing output 10 2 6 3 Trigger ready output The trigger ready output is useful to make the frame intervals as short as possible in external trigger mode For example when the camera is working in the edg
50. ted 2 The warranty only covers defects in the materials and manufacturing of the system You may be liable for repairs during the warranty period in the event of a natural disaster or if you handle the system contrary to the instructions in this manual use it without due caution or try to modify it 3 We will repair the system or replace it subject to availability free of charge within the terms of the warranty REPAIRS 1 If you notice anything wrong with the camera confirm whether or not it is malfunctioning by referring to the troubleshooting checklist in this instruction manual You must first clarify the symptoms in order to avoid any misunderstanding or error 2 If you have any trouble or are unclear about anything contact Hamamatsu subsidiary or local distributor giving the product name serial number and details of the problem If Hamamatsu Photonics consider the problem to be a malfunction we will decide whether dispatch an engineer or have the camera returned to us for repairs 68 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 17 CONTACT INFORMATION Manufacturer HAMAMATSU PHOTONICS K Systems Division 812 Joko cho Higashi ku Hamamatsu City 431 3196 Japan Telephone 81 53 431 0124 Fax 81 53 435 1574 E mail export sys hpk co jp Local contact information worldwide could be found under www hamamatsu com The contents of this manual are subject to change without notice
51. th is 1 us to 10 The relation between the parameter which can be set with each reference signal and an output signal becomes as shown below Output signal Camera outputs a pulse after certain delay from the end of sensor readout Also the pulse width can be set Camera outputs a pulse after certain delay from the beginning of readout Also the pulse width can be set Camera outputs a pulse after certain delay from the ending of readout Also the pulse width can be set Externaltrigger Sensor readout i L Delay Pulse Width Pulse Width Figure 10 23 Programmable timing output 48 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 External trigger 1 Pulse Width Figure 10 24 Programmable timing output referenced with Hsync When you choose Hsync for the reference of programmable timing output camera can output some pulses before start the exposure It is called as Pre Hsync You can set the number of Pre Hsync with a software command 10 4 REAL TIME CORRECTION FUNCTIONS There are a few pixels in CMOS image sensor that have slightly higher readout noise performance compared to surrounding pixels The camera has real time variant pixel correction features to improve image quality The correction is performed in real time without sacrificing the readout speed at all This function can be turned ON and OFF Default is ON HAMAMATSU 49 C11440 22CU C11440 22CU01 Instruction manual Ver
52. th only Camera Link interface operation And the fastest frame rate is 49 frames s Figure 10 16 Normal area mode Figure 10 17 Light sheet mode 1 Top to bottom read out The data is read out from the top to the bottom line 2 Bottom to top read out The data is read out from the bottom to the top line Figure 10 18 Top to bottom read out Figure 10 19 Bottom to top read out Light sheet mode supports three operation modes Also light sheet mode supports sub array read out 1 Free running mode 2 Edge trigger mode 3 Start trigger mode 44 HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 10 3 2 FRAME RATE CALCULATION Calculation formula 1 Exp1 Vn 10 x1H Vn Number of vertical line the number of lines from the middle of the sensor 1 9 7 1510108 1H HLN 266 x 10 HLN 2592 to 26600000 Value of frame rate Horizontal width x Vertical width Frame rate frame s Size and position for sub array read out can be configured according to the table below Settings Vertical 4 lines steps Position 10 3 3 READ OUT TIME OF THE HORIZONTAL LINE Read out time and exposure time can be varied with light sheet mode Vn Number of vertical line 1H HLN 266 10 9 7436 us to 100 ms HLN 2592 to 26 600 000 The range of exposure time with light sheet mode is as shown below The maximum exposure time can be decided according to the readout time of a frame
53. the following 1 Blow the dust from the glass window with an air duster 2 Moisten a lens cleaning paper with a little ethanol and wipe over center area of the window gently Please use a plastic tweezers and take extra care not to scratch glass window with the tweezers Even with plastic tweezers there is possibility to make scratch glass window in case tweezers touch it 3 Confirm whether dust is not left Attach the camera to an optics and check if there is dust or not under the uniform light condition If there is dust on the image please clean the glass window again HAMAMATSU 51 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 12 2 12 2 1 INFORMATION ON COOLING WATER FOR THE CIRCULATING WATER COOLER e Regarding handling cooling water and circulating water cooler please refer to instruction manual attached to the circulating water cooler e Do not use hard water for cooling It cause inside of cooling water circulating path to be calcified or corroded and it result lower flow rate or water flow stop When using hard water please conduct a process to soften water before use it WHEN USING COOLING WATER OTHER THAN RECOMMENDED Pure water e Deionized water is not appropriate for cooling water There is possibility that deionized water absorb component of cooling water path and it may cause corrosion In addition deionized water is easy to be polluted and cause impurity slimi
54. to ensure correct and safe use Failure to observe one of these notes might impair the performance of the camera This symbol indicates a cautionary item that should be obeyed when handling the camera Read the contents carefully to ensure correct and safe use This symbol indicates an action that is forbidden Read the contents carefully and be sure to obey them This symbol indicates a compulsory action or instruction Read the contents carefully and be sure to obey them HAMAMATSU 1 C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 Power supply Use the camera with the voltage indicated on the rating sticker Using a different voltage can damage the camera and lead to fire or electric shock Cables Be careful not to place heavy objects on cables or bend it excessively Doing so can damage the cable and lead to fire or electric shock Power supply cord Use the accessory of the AC adapter when this camera is used Do not touch the plug with wet hand Doing so can lead to electric shock Do not attempt to dismantle or modify the camera Doing so can also lead to damage and even injury as some internal components become very hot Only touch parts as indicated in this manual Do not insert a foreign substance into the camera Do not allow foreign objects such as combustible substances metal objects or water to get inside the camera They can damage the camera and lead to fire or electric shock If an abnormality occu
55. ts of Camera Link connector 2 SDR 26 Camera connector Frame grabber connector Channel Link signal Inner Shield 0 Y1 Y2 Yclk Y3 Terminated 20 21 Z2 Zclk Z3 Inner Shield Inner Shield 0 Y1 2 Yclk Y3 Terminated 20 21 22 Zclk Z3 Inner Shield HAMAMATSU C11440 22CU C11440 22CU01 Instruction manual Ver 1 5 3 Camera Link bit assignments 28 bit solution pin name Plug No 1 Plug No 2 Channel Link X Plug No 3 Channel Link Y Port Channel Link 7 Port Port TxINO TxIN1 TxIN2 TXIN3 TXIN4 TXIN5 TXIN6 TXIN7 TxIN8 TxIN9 TXIN10 TXIN1 1 TXIN12 TXIN13 TXIN14 TXIN15 TXIN16 TXIN17 TXIN18 TXIN19 TXIN20 TxIN21 TxIN22 TxIN23 TxIN24 TxIN25 TxIN26 TxIN27 Port AO Port A1 Port A2 Port A3 Port A4 Port A5 Port A6 Port A7 Port BO Port B1 Port B2 Port B3 Port B4 Port B5 Port B6 Port B7 Port Port C1 Port C2 Port C3 Port CA Port C5 Port C6 Port C7 LVAL FVAL Port DO Port D1 DO 0 DO 1 DO 2 DO 3 DO 4 DO 5 DO 6 7 8 9 10 14 12 13 14 DO 15 MSB D1 0 D1 1 D1 2 D1 3 D1 4 D1 5 D1 6 D1 7 LVAL FVAL D1 8 D1 9 Port D2 Port D3 Port D4 Port 05 Port 06 Port 07 Port EO Port E1 Port E2 Port Port E4 Port E5 Port E6 Port E7 Port FO Port F1 Port F2 Port F3 Port F4 Port F5
56. ulating water cooler Handle the circulating water cooler and the cooling water according to an instruction manual of the circulating water cooler Proper performance may not be achievable if a non recommended circulating water cooler is used Start water cooling and water cooping in operation e Confirm the water is flowing before starting the camera cooling and that the camera does cool Keep 1 0 L min flow rate for water circulation Do not stop coolant while the camera is working Cooling water hose The hose has a blue hose Internal diameter 4 mm External diameter 6 mm and a gray hose Internal diameter 8 mm External diameter 13 5 mm Figure 8 1 If the hose size on circulating water cooler is the same as blue hose remove gray hose from the joint part The gray hose can be removed when blue hose is pulled with pushing the button of the joint on gray hose Figure 8 2 Figure 8 1 Figure 8 2 Connection of the cooling water hose e Follow the instruction in Section 8 2 CONNECTION OF WATER A CAUTION COOLING HOSES and Section 8 3 DISCONNECTION OF WATER COOLING HOSES to connect disconnect the hose Stop water circulation when connecting disconnecting the hose and turn off the power of the camera and the circulating water cooler e Confirm that cooling water stops Prepare water absorption sheet such as Waste Towel or so and catch pan in order to avoid water drop or water splash Deteriorat
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