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Multiskan FC - Thermo Scientific

1. Thermo Scientific Multiskan FC User Manual Rev 1 4 Cat no N07710 Copyright Copyright 2009 Thermo Fisher Scientific Corporation First edition published in 2008 All rights reserved Printed in Finland Reproduction of the accompanying user documentation in whole or in part is prohibited Patents This product is protected by the following patent US 6111636 Device for Measuring Optical Density Trademarks Immulon Microtiter Multiskan and SkanIt are registered trademarks of Thermo Fisher Scientific All other trademarks and registered trademarks are the property of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions No liability for consequential damages Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use this product Power failure The system requires uninterrupted power supply in order to operate correctly Thermo Fisher Scientific has no
2. Contents Chapter 16 Ordering Information ooooooooooooooooooooooooooooc 115 STSCI ELC E tik canta ena 115 List of spare parts and accessories senat 116 List of Thermo Scientific plates Mma 116 Vera Oo ea NN Br 117 Appendix A System LOO an Ann An PP 119 Appendix B Certificate of Decontamination o oooooo oom om 121 10 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Chapter 1 Introduction to the Multiskan FC The Multiskan FC Figure 1 1 is a high quality filter based microplate photometer It is used to measure absorbance from appropriate 96 or 384 well plate formats in the wavelength range of 340 to 850 nm Optional incubation up to 50 C can be used The instrument also allows shaking The instrument has a sophisticated internal software It can also be connected to Thermo Scientific SkanIt Software for Multiskan FC Furthermore the Multiskan FC can be connected to plate handling devices The Multiskan FC is available in the following configurations e Multiskan FC e 96 well plate reading with internal shaking e Multiskan FC with incubator e 96 and 384 well plate reading with shaking and incubation Figure 1 1 Multiskan FC microplate photometer Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 11 Introduction to the Multiskan FC Intended use Intended use 12 Thermo Scientific Multiskan FC User Manual The Multiskan FC is a microplate photometer for me
3. It is also possible to get this error if either one of the measure head or plate motors has lost steps so that the position is wrong Contact service Do not try to set the instrument serial number Using the PUT command delete some unused user Thermo Scientific Multiskan FC User Manual 111 Troubleshooting Guide Error and warning codes Cont Code Explanation This error may be reported in response to a PUT command 13 such an error during startup that the execution of the MEA and SCA commands is precluded 14 The distance between plate wells is too short for scan measurement 15 The sampling time for a single result is too long for scan measurement The plate cannot be moved as slowly as the sampling time requires 16 Failed to adjust a suitable lamp intensity for a filter 17 Too high dark signal level The dark level is checked once for each measured plate row column 18 Measure signal saturation 19 No filters defined 20 The USB memory stick printer interface is not working 21 The USB memory stick printer interface firmware is corrupted 22 XY table position calibration failed This may be reported after startup 23 Command parser optimization failure 24 Temperature reference is out of limits Incubation is not available if this error is reported 25 Parameters memory not found 26 Parameters memory erase failure 27 Parameters memory write failure 28 Temperature is not changing 29 One or more heat
4. Performing self diagnostics 2 Do not plug unplug the printer cable during Importing Exporting data from to the USB memory stick 3 Do not plug unplug the USB memory stick during Printing or Performing self diagnostics A To start a ready made protocol shown on the info text bar with the quick keys F1 F3 follow these steps Results Settings Untitled Plate format 96 Wells Measurement 405nm 1reading Shaking No Incubation No OK Edit Demot Demo2 Demo3 1 Press for example the F1 key Demo in the Main menu 2 If the plate carrier is inside the instrument press the PLATE in out button Insert the assay microplate so that the Al corner is positioned in the top left corner of the plate carrier Figure 6 18 Figure 6 18 Multiskan FC plate carrier Thermo Scientific Multiskan FC User Manual 61 Starting Ready made Protocols Starting a ready made protocol from the list 2 Z O N gt ct O oE E ay Starting a ready made protocol from the list A ok a V E 62 Thermo Scientific Multiskan FC User Manual Press the START button a Enter the unknown count with the number keys 5 Press the START or OK button to accept the selection and to start the measurement Note If you want to cancel the run press the F2 key A 6 The microplate is measured and the results are automatically calculated according to the predefined protocol
5. 4 Select Export Results As Text or Print Results and press the OK button 5 The Define export Print box is opened Select the information you want using the Down arrow key and tick the check box with the OK button f Processing Results y Settings Define export Run information Instrument status Plate layout Quality control Results as matrix Results as list Move OK Select Accept Cancel 6 Press the F1 key to accept the desired data Data is exported to the USB memory stick or printed to the external printer depending on the selection Run information includes all protocol and processing parameters The naming of an export file is automatic with a running number that is the instrument searches for the first number available Note The data format should be UTF 8 when the txt file is opened in for example Microsoft Excel A Note The internal software uses 32 bit floating point numbers in processing A The calculated results or stored protocols can be exported to the USB memory stick via the USB port Figure 2 8 on page 18 by using the FILE key Runs including protocol information and data can be exported Protocols can be exported and imported to another Multiskan FC instrument with the same configuration Import of runs is only possible to the same instrument as backup In the main level of Results the results or protocols to be exported can be defined by FILE Export A pro
6. H half bandwidth 108 header 59 90 how to decontaminate the instrument 85 93 95 96 97 105 121 how to install the Multiskan FC 13 14 18 19 94 109 how to maintain the instrument 62 64 93 how to pack for service 13 105 how to shut down 85 93 how to switch on 18 how to unpack 13 Ignore from beginning 38 41 43 44 45 46 Ignore from end 38 41 43 44 45 46 importing a protocol 83 importing results or protocols 82 83 Thermo Scientific Multiskan FC User Manual 125 incubation 11 24 29 30 34 35 65 66 67 112 parameters 29 30 34 67 incubator 11 24 29 34 66 87 109 115 installation 3 13 14 16 18 19 33 100 109 110 category 109 110 installing the filter wheel 16 100 instrument settings 58 90 101 intended use 3 12 internal memory 24 interpretation 29 35 50 51 52 53 65 73 74 79 parameters 65 introducing a filter to the internal software 33 89 K keypad 19 25 53 54 94 107 keys 25 26 29 53 54 61 62 63 66 67 69 70 73 74 75 76 80 87 89 107 kinetic 24 26 33 34 35 37 38 39 40 41 43 51 52 54 56 71 79 113 123 calculations 35 37 38 39 40 71 measurements 24 26 34 37 123 rate 38 41 43 kinetic measurements 24 26 34 37 123 Kinetic rate 38 41 43 L lamp 14 17 23 93 97 100 101 102 103 104 105 108 111 112 116 lamp and filter wheel chamber 14 17 97 10
7. 3 4 5 The instrument has to be powered ON Press the PLATE in out button so that the plate carrier goes in Switch the power OFF Open the lamp and filter wheel chamber cover Push the transport lock bar through the hole in Figure 13 26 into the hole in the measurement head Figure 13 26 Refitting the transport lock bar into the measurement head Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 103 Maintenance Refitting the transport lock 104 Thermo Scientific Multiskan FC User Manual 6 Push the transport lock bar in as far as possible Figure 13 26 7 Fasten the transport lock bar and tag clockwise using the Allen key supplied Figure 13 27 Ensure that the transport lock bar is completely tightened Figure 13 27 Fastening the transport lock 8 Close the lamp and filter wheel chamber cover The transport lock bar and tag are now refitted Figure 13 28 CAUTION Remove Transport Lock Before Use Figure 13 28 Transport lock bar and tag refitted Thermo Fisher Scientific Maintenance Maintaining a system log Maintaining a A system log which includes a short summary of the use maintenance procedures error messages and other information about the use of the system log system can be very useful in properly maintaining the system Refer to Appendix A System Log Copy the table as many times as necessary but leave the blank original insi
8. Note During the run you can press the STOP button to abort the run A 7 Press the F2 key to close the result table and then press the Left arrow key twice to return to the Main menu Note The main parameters are locked when a run measured data for this protocol exists A Refer to Chapter 13 Maintenance To start a ready made protocol from the protocol list follow these steps Caution 1 Do not switch the power off during Performing self diagnostics 2 Do not plug unplug the printer cable during Importing Exporting data from to the USB memory stick 3 Do not plug unplug the USB memory stick during Printing or Performing self diagnostics A 1 Press the OK button on the Protocol row in the Main menu OR Press the FILE key in the Main menu and select Open using the Down arrow key and then press the OK button Thermo Fisher Scientific Starting Ready made Protocols starting a ready made protocol from the list Created 17 06 2008 13 00 14 17 06 2008 13 01 16 17 06 2008 13 02 02 17 06 2008 13 02 34 17 06 2008 13 02 50 17 06 2008 13 03 02 17 06 2008 13 03 40 Helicobacter 17 06 2008 13 04 44 HIV 1 2 17 06 2008 13 05 30 Cell prolif Clamydia Demot Demo Demo3 Endotoxin Example protocol list 17 06 2008 13 02 02 17 06 2008 13 02 34 17 06 2008 13 02 50 16 06 2008 09 26 14 17 06 2008 13 03 40 17 06 2008 13 04 44 17 06 2008 13 05 30 MTT 17 06 2008 13 05 54 17 06 2008 13 05 54 Move
9. OK Edit 4 Prev Next Cancel Remove 3 Select the empty filter position by pressing the Right arrow key until the filter wheel on the display rotates to the empty position and the text Empty is shown Aa Press the OK button Enter the wavelength of the filter for example 492 with the number keys and then press the OK button 5 Press the F1 key to accept the filter settings Note The instrument starts the filter initialization A 6 Press the Right arrow key to return to the Main menu Thermo Scientific Multiskan FC User Manual 89 Modifying Settings Printer Removing a filter Printer Startup 90 Thermo Scientific Multiskan FC User Manual You can remove a filter from the instrument settings by selecting the position and pressing the F3 Remove key Note The filter must physically be removed from the filter wheel A You can add a header in the Printer parameter window for example the laboratory information to all of the reports to be printed You can do this by selecting Yes for the Enable header The maximum length of the header is about 50 characters In the Startup parameters the Plate position setting defines the plate carrier position after the instrument is switched on You can set the startup temperature The instrument starts to warm up to the set temperature immediately after it has been switched on Note The set startup temperature is only activated when the instrument is powered
10. si AN N Select the Shaking row in the Main menu using the Down arrow key and press the OK button Press the OK button at the Mode item and select for example Before measurement as the shaking mode using the Down arrow key and then press the OK button Select the Speed item using the Down arrow key and press the OK button Select for example Fast as the shaking speed using the Down arrow key and then press the OK button Select the Time hh mm ss item using the Down arrow key and press the OK button Select for example 20 seconds 00 00 20 as the shaking time by using the number keys and then press the OK button Press the F1 key to accept the selection and return to the Main menu You can set the incubation time and temperature in the Incubation window Note The Incubation row is only active with the Multiskan FC with incubator model A Thermo Fisher Scientific Thermo Fisher Scientific Protocol Creation Examples Programming incubation Protocol Untitled Sy Plate format 96 Wells l Measurement 405nm 1 reading D gt Shaking No IEB Incubation No Mo OK Edit Demo1 Demo2 Demo3 Select the Incubation row in the Main menu using the Down arrow key and press the OK button Press the OK button on the Temperature row and use the Down arrow key to select the preferred temperature from the list The temperature range is from 25 to 50 C Then press the OK button 3 If Wait fo
11. switched on The temperature set in the protocol overrules the startup temperature If the protocol does not include incubation the startup temperature remains A Thermo Fisher Scientific Chapter 12 Emergency Situations Handling In case there is any abnormal situation during the operation such as fluids spilling inside the instrument follow these steps emergency ani p situations 1 Switch OFF the instrument Figure 3 10 on page 21 2 Unplug the instrument immediately from the power supply Figure 2 8 on page 18 3 Carry out appropriate corrective measures However do not disassemble the instrument 4 Ifthe corrective measures taken do not help contact authorized technical service or your local Thermo Fisher Scientific representative Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 91 Emergency Situations Handling emergency situations 92 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Chapter 13 Maintenance Regular ond Contact local authorized technical service or your local Thermo Fisher Scientific representative for assistance if necessary preventative i ji maintenance Maintenance This chapter contains an outline of the points mentioned in the checklist checklist below Table 13 13 Table 13 13 Maintenance checklist Item gt sy x zi 3 A gt Keep the instrument free of dust See General on page 94 v Avoid disturbing any of the opt
12. thermo com readingroom In an effort to produce useful and appropriate documentation we appreciate your comments on this user manual to your local Thermo Fisher Scientific representative Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 3 About This User Manual safety symbols and markings Safety symbols and markings Safety symbols and markings used on the Multiskan FC Bc Gal leh O 4 Thermo Scientific Multiskan FC User Manual These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated The following symbols and markings appear on the type label and the instrument itself Power ON a Power OFF a Serial number a Catalog number a Date of manufacture a Consult instructions for use a WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC A A black label with the following text Figure 3 10 on page 21 CAUTION WARNING DISCONNECT SUPPLY BEFORE SERVICING and AVERTISSEMENT COUPER L ALIMENTATION AVANT L ENTRETIEN ET LE DEPANNAGE 4 Thermo Fisher Scientific About This User Manual safety symbols and markings Warning and other The following symbols and markings appear in this user manual markings used in the documentation Warning Risk of electric shock A Warning Biohazard risk A Warning Hot surface risk o
13. 0 001 Abs 1 0 3 3 Abs 2 3 4 Abs CV lt 0 2 0 3 3 Abs CV lt 1 0 3 4 Abs Approx 30 s 6 s 96 well plate fast mode 12 s 96 well plate normal mode 15 s 96 well plate fast mode dual wavelength 11 s 384 well plate fast mode 33 s 384 well plate normal mode 25 s 384 well plate fast mode dual wavelength Instrument is stable due to autoblanking procedure Thermo Fisher Scientific Technical Specifications safety specifications Table 14 17 Incubator Performance specifications Incubator Temperature range Ambient C 4 C to 50 C Liquid warm up time Liquid warm up time lt 40 min from 25 C to 37 C 96 well plate Uniformity Safety specifications In conformity with the requirements Thermo Fisher Scientific 200 ul water well 1 C across the plate at 37 C This section describes the safety specifications for the Multiskan FC instrument Multiskan FC bears the following markings Type 357 100 240 Vac 50 60 Hz 100 VA max CE mark CSA monogram Multiskan FC conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B July 2004 2002 96 EC Waste of Electrical and Electronic Equipment Safety performance EN 61010 1 2001 Ed 2 including US and CA National differences EN 61010 2 010 2003 Ed 2 EN 61010 2 101 2002 Partic
14. 59 61 62 81 90 113 printing or exporting data 81 program overview 29 programming 33 34 37 53 65 66 68 71 72 73 a plate layout 37 68 Thermo Fisher Scientific a wavelength 33 65 an interpretation 53 73 calculations 71 72 incubation 66 precalculations 71 shaking 34 66 protocol 3 18 20 26 27 29 30 31 33 35 36 39 48 50 51 53 54 55 56 58 61 62 63 64 65 68 73 75 76 77 79 82 83 84 90 112 0 quality control 12 29 35 53 54 57 75 79 parameters 53 75 guantitative assay 51 raw data 29 40 54 55 79 Reaction 38 42 44 45 Readings from 38 45 ready made protocols 26 61 62 refitting the transport lock 103 105 releasing the transport lock 14 remote control 19 27 result handling 55 71 results in list format 29 57 S saving a new active protocol 65 76 service 13 23 91 93 94 103 105 106 111 112 113 114 115 pack for 13 105 setting the date 87 Settings parameters 59 shaker 24 34 107 shaking 11 24 29 30 34 65 66 107 speed 24 34 66 shutdown 85 93 single measurement 26 123 source data 40 51 52 73 spare parts 116 specifications 3 106 107 108 109 general 107 performance 108 109 safety 106 109 speed 24 26 34 66 starting a ready made protocol 61 62 Thermo Fisher Scientific from the list 62 with the guick keys F1 F3 61 startup 18 23 29 58 59 87 90 1
15. Demo2 Demo3 System settings The Multiskan FC instrument s internal software version serial number and model are shown in the System settings window Model 1 refers to Multiskan FC and Model 2 to Multiskan FC with incubator You can set the date date and time format and language in the System settings parameter window s OK Select the item to be changed with the Up and Down arrow keys and press the OK button Setting the date You can change the date in the System settings The Left and Right arrow keys are used to move from one position to Lee another in the Date setting Enter the values with the number keys to i Changing the You can set the internal software language by moving to Language and language selecting the preferred language from the drop down list To change the internal software language follow these steps Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 87 Modifying Settings Filters 1 Inthe Main menu select the Settings menu using the Left arrow key 2 Select the System row and press the OK button 3 Select the Language item using the Down arrow key and press the OK button Settings System parameters Date 08 07 2008 10 57 38 G Multiskan FC Date format SMEERT Version 10048 X Chinese SN Empty Time format Frangais French Model 2 Deutsch German n O Language Hakia Japanese Portugu s Portuguese Pyccknn Russian Espa ol Spanish
16. Move OK Select 4 Select the internal software language for example Francais using the Down arrow key and then press the OK button to accept the selection 5 Press the F2 key to confirm the selection and to close the system parameters 6 Return to the Main menu using the Right arrow key Fi Ite FS Multiskan FC comes with installed filters of 405 mm 450 mm and 620 mm You can introduce a filter to the internal software by moving to the Filters settings The filter wavelength must be between 340 and 850 nm The Filters settings window shows the current filters of the filter wheel 88 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Introducing a filter to the internal software Thermo Fisher Scientific Modifying Settings Filters This section provides an example on how to enter the information of an additional filter which has been added to the filter wheel To introduce a filter to the internal software follow the given steps Note Before introducing the additional filter to the internal software make sure the filter is physically inserted into the next free filter position in the filter wheel and the filter wheel is placed into the filter wheel slot A 1 Inthe Main menu select the Settings menu using the Left arrow key 2 Select the Filters row using the Down arrow key and press the OK button settings Filter settings Wavelength nm 405 Comment
17. OK Load 2 Select the ready made assay protocol you want to run from the protocol list using the Down arrow key and then press the OK button vE Note The protocol name selected is shown on the Protocol row in the Main menu A B 3 If the plate carrier is inside the instrument press the PLATE in out button Insert the assay microplate so that the Al corner is positioned in the top left corner of the plate carrier PLATE in out 4 Press the START button Enter the unknown count with the number keys ct 6 Press the START or OK button to accept the selection and to start the measurement 23 EH onimo m Note Ifyou want to cancel the run press the F2 key A 7 The microplate is measured and the results are automatically calculated according to the predefined protocol Note During the run you can press the STOP button to abort the run amp Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 63 Starting Ready made Protocols Starting a ready made protocol from the list a Note The main parameters are locked when a run measured data for 8 Press the F2 key to close the result table and then press the Left arrow key twice to return to the Main menu this protocol exists A Refer to Chapter 13 Maintenance 64 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Chapter 7 Protocol Creation Examples This section provides examples on how to
18. Qualy control MO ani Mana 57 FRESE CS LOIS TO KENA Bani aan aan enakan ema e nastar 57 Seins MENT isai A oie 58 Chapter 6 Starting Ready made Protocols oom 61 Starting a ready made protocol with the quick keys F1 F3 61 Starting a ready made protocol from the list ooo 62 Chapter 7 Protocol Creation Examples o oooooo oo mom mmaa 65 Opening Sa NEW protocol hama ea ea aa asi 65 Programming Wav eni ana EA 65 Programming shaking alan 66 Programming INCU DATON nana Bebas 66 Programmins plate ayol ayia maan aa us 68 Programming calculations and interpretations o 71 Programming precaleulations3 soal sasa 71 Programming calculations oooooo WWW 72 Programming an INLET PLEta ON see aan alan Ben igs Aadine asOG rile tothe protodol naa 75 Saving a new active Protocol Ka Ea 76 Chapter 8 VIEWING Resume kak kan 79 Yen resume E 79 Chapter 9 Printing Exporting and Importing o ooooo oooo ooo oo om mmm 81 8 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Chapter 10 Chapter 11 Chapter 12 Chapter 13 Chapter 14 Chapter 15 Thermo Fisher Scientific Contents Prinonosor CX POLIS data sman 81 EXPOnting Ai protocol sange ate hua ecards 82 Imporunga protocol naa an 83 ON ai 85 SIMUL do an erate 85 Modifying Settings o ooooooo oo oom 87 DY Ce NSU IN Osha Tana Suess cps NN Nan An TA Na 87 SANNE eh el scarey a een Re
19. This applies to both the protocol and startup temperature Contact service Contact service Contact service Select filter 1 Thermo Fisher Scientific Cont Code 52 53 54 55 56 b 08 59 60 61 Code 100 101 102 Warnings and Thermo Fisher Scientific Explanation Filter information in the protocol does not match the filter wheel The file already exists The file does not exist The media is full End of file Other file error none of the previous Firmware update aborted by user Printer not attached The printer is out of paper Printing error Troubleshooting Guide Warnings and cautions Suggested action Edit the protocol or add the correct filter to the filter wheel Save with another file name Contact service You have to delete some protocols or runs If Defragmenting file system messages start to appear on the display you should already at this Stage make more space by deleting some protocols Or runs Contact service Contact service Connect the external printer Add paper to the printer Retry and if the error persists contact service Table 15 19 Warning codes reported Explanation Unable to comply with the defined kinetic interval The timer referenced in the WAI timer command is not anymore running Your timing requirement is not met Temperature is not changing cautions the user Suggested action The minimum timing depen
20. a new active protocol that is open in the menu follow these steps 1 Press the FILE key in the Main menu 2 Select Save As using the Down arrow key and press the OK button The Save Protocol As dialog opens 3 Enter the protocol name for example Test by using the number and letter keys and then press the OK button Thermo Fisher Scientific Protocol Creation Examples Saving a new active protocol Processing Plate layout Yes Note You cannot use the protocol name Undefined A Note The results of an Undefined protocol are not saved unless you save the active protocol including the measured run data with a new name A Der Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 77 Protocol Creation Examples Saving a new active protocol 18 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Chapter 8 Viewing Results Viewing results The results can be viewed in several formats depending on the protocol settings raw data in list and table format calculated results in list and table format and graphs calibration or kinetic curves You can disable enable samples and sample replicates from the raw data in table format After the run has finished the data view of a run is automatically shown The order of the data views results to be shown is according to the following list depending on which calculations were used in the protocol e Assay
21. ao mM OOOCOOCOOs Q O O O x OK Add Accept Cancel Edit 2 Press the OK button to start filling the plate from well A1 3 Select the Well type item in the Add series starting from well Al window and press the OK button 4 Select for example a Blank sample using the Up arrow key and then press the OK button Thermo Fisher Scientific Thermo Fisher Scientific Y F3 Protocol Creation Examples Programming a plate layout Processing Add series starting from well Well type Fill direction Number of Move OK Select Cancel 5 Press the F1 key to accept the selection and return to the Layout window well A2 oN Select well B1 using the Down and Left arrow keys and press the OK button to start filling the plate from well B1 7 Select the Well type item in the Add series starting from well B1 window and press the OK button 8 Select for example a Calibrator sample using the Up arrow key and then press the OK button 9 Select the Number of item using the Down arrow key and press the OK button 10 Select for example 3 by using the number keys and then press the OK button Sh ee Processing 1 Add series starting from well B1 Well type Calibrator Fill direction Down Replicate direction Down Number of B Replicates 1 4 gt Move OK Select Cancel 11 Press the F3 Conc key to program the concentrations for the calibrators 12 Se
22. be entered would be Sample Raw BLA Operator lt Constant 0 100 The following example shows how to add a Quality Control QC rule to a protocol i Processing Quality control parameters Enable quality control Raw BLA lt 0 100 24 gt Move OK Edit Accept Cancel 1 Select the Quality Control row in the Processing menu using the Down arrow key and press the OK button 2 Press the OK button on the Enable quality control and select Yes using the Up arrow key and then press the OK button 3 Select the Rule 1 row using the Down arrow key and press the OK button 4 Select Sample and press the OK button Thermo Scientific Multiskan FC User Manual 75 Protocol Creation Examples Saving a new active protocol gt E vE Gg H ok WXYZ Saving a new active protocol Pr A c a 16 Thermo Scientific Multiskan FC User Manual A A 10 Select BLA blank and press the OK button Select Raw and press the OK button Select Operator using the Right arrow key and press the OK button Select lt using the Down arrow key and press the OK button Select Constant using the Right arrow key and press the OK button Enter the constant for example 0 100 using the number keys and press the OK button Press the F1 key to accept the Rule 1 formula Press the F1 key to accept the Quality control parameters and to return to the Processing menu To save
23. filter ray Spring position holding screws Figure 13 21 Filter wheel parts Figure 13 22 Individual filter showing the arrow a Note Arrow pointing up Figure 13 23 Inserting a filter into the next free position in the filter wheel Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 99 Maintenance Adding individual filters to the filter wheel 100 Thermo Scientific Multiskan FC User Manual p Insert a new filter or an additional optional filter into the next free position in the filter wheel Figure 13 23 on page 99 When inserting the filter the arrow must be pointing upwards as in Figure 13 23 on page 99 The wavelength of the filter is marked on the filter package as well as on the filter side that is the three digits before the last digit of the number sequence xxxxx NNNXx where NNN is the wavelength The filter positions are marked on the other side of the filter wheel The filter wheel has at present three filters factory installed Table 13 14 Note Do not change the positions of the factory or earlier installed filters Insert the optional filters into filter wheel positions after the earlier installed filters A Table 13 14 Example of the filters in the filter wheel positions 1 8 Filter wheel position Wavelength nm 1 CO Y O or amp S WY RO 405 example of factory installed filter 450 example of factory installed filter 620 example of factory i
24. for the samples are needed A Note The Replicate number is the same for samples for example for When filling the plate with the Add series function note that adding a group of samples of the same type to another location will delete the first group If you want to add the same well type into several separate locations on the plate layout use the Edit function For instructions on how to program the plate layout refer to Programming a plate layout on page 68 Preprocessing You can define the preprocessing parameters in the Preprocessing parameters Window Preprocessing includes two different types of data processing precalculations and kinetic calculations Processing N Preprocessing parameters Precalculation Kinetic Meal Mea2 Meal Mea2 Meal Mea2 Meaz Meat Mea2 Meat Move OK Select Cancel Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 37 Menus and Parameters Processing menu 38 Thermo Scientific Multiskan FC User Manual Preprocessing is used with dual two wavelength measurements and kinetic measurements for example to subtract the reference wavelength data in dual measurements or to reduce data for kinetic measurements in kinetic calculations Table 5 8 Processing Preprocessing parameters Processing Preprocessing Preprocessing L Pre Calculation L No L Meal Mea2 L Meal Mea2 L Meal Mea2 L Meal Mea2 L Mea2 Meal L Mea2 Meal L Kine
25. functions and provides data reduction as well as reporting functions USB 1 1 2 0 compatible 96 and 384 well plates Linear shaking 3 speeds Slow 5 Hz 15 mm Medium 11 Hz 3 mm and Fast 20 Hz 8 1 mm Thermo Scientific Multiskan FC User Manual 107 Technical Specifications Performance specifications Performance This section provides the performance specifications for the relevant ag measurement technique and other instrument capabilities specifications Table 14 16 Photometry Performance specifications Photometry Optical system Wavelength range Filters Half bandwidth of filters Wavelength accuracy Detector Linearity 96 well plate 405 nm Linearity 384 well plate 405 nm Measurement range Absorbance resolution Accuracy 405 nm normal mode Precision 405 nm normal mode Startup time Measurement time Long term stability 108 Thermo Scientific Multiskan FC User Manual Quartz halogen lamp Osram 64222 6V 10W interference filter in the filter wheel fiber fiber end optics photodetector signal processing 340 850 nm 8 position filter wheel The filters are optional The standard filters are 405 nm 450 nm and 620 nm Additional filters can be ordered separately 3 9nm 2nm One silicon photodetector 0 4 Abs 2 normal mode 0 3 Abs 2 fast mode 0 3 Abs 2 with normal mode 0 2 5 Abs 2 with fast mode 0 6 Abs
26. instrument A Immediate Although the Multiskan FC is constructed from high quality materials you must immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage and wipe with deionized distilled water 94 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Cleaning a filter A Cleaning of the instrument A gt e Thermo Fisher Scientific Maintenance Cleaning a filter To clean filters follow these steps 1 Remove particles for example dust with clean pressurized air 2 If necessary clean the filters with a lintfree tissue soaked in 70 ethanol Caution Do not use any other liquids to clean the optics Avoid any harsh treatment A 3 Wipe over the filter in a straight movement from inside to out Do not use any circular movements when cleaning the filter 4 Repeat the procedure if the filter is not clean Clean the instrument regularly as stated below Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur A 1 Turn the power OFF and unplug the instrument 2 Use disposable gloves 3 Clean the instrument outside and the plate carrier with a soft cloth dampened with water or mild detergent 4 Ifyou have spilt infectious agents
27. kinetic calculations or a curve fit Thermo Scientific Multiskan FC User Manual 39 Menus and Parameters Processing menu Kinetic calculations 40 Thermo Scientific Multiskan FC User Manual The most common example of precalculation is the subtraction of the reference wavelength where absorbance values of Measurement 2 measured with Filter 2 is subtracted from the absorbance values of Measurement 1 measured with Filter 1 Also the sum multiplication and ratio precalculations between the absorbance values of Measurement 1 measured with Filter 1 and the absorbance values of Measurement 2 measured with Filter 2 are available Meal Mea Meals MeaZ Meal Mea MWeal Nea Mea Meal Meal Meal Kinetic calculations can be used when the number of readings is more than 1 in the measurement parameters see Measurement parameters on page 33 Also the results of a kinetic calculation can be used as source data for further calculations The available kinetic calculation types are e Average rate e Maximum rate e Time to maximum rate e Time to change e Maximum of well e Time to maximum Average rate Average rate is also known as normal rate The average kinetic rate slope of the absorbance Abs vs time curve is calculated by linear regression linear least squares method or LLS using all the measurement readings within the selected raw data and time range You can define the following settings Thermo Fisher
28. on the instrument decontaminate y p g the instrument Refer to Decontamination procedure on page 96 Caution Do not use any solutions containing hypochlorite such as bleach on any of the stainless steel surfaces as this may cause permanent damage to the finish A Thermo Scientific Multiskan FC User Manual 95 Maintenance Disposal of materials Disposal of materials Decontamination procedure A 96 Thermo Scientific Multiskan FC User Manual Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Refer to local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used plates disposable gloves syringes disposable tips and so on as biohazardous waste Be cautious and always use gloves A If you have spilt infectious agents carry out the decontamination procedure Warning The decontamination procedure should be performed by authorized trained personnel in a well ventilated room wearing disposable gloves protective glasses and clothing A Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed It is strongly recommended to perform the complete decontamination Sly procedure before relocating the instrument from one laboratory to another Example of decontaminants e Ethanol 70 e Virkon
29. operator e MINUS SIGN operator e SLASH SIGN operator e and pare found under the 1 key Use the CLEAR C key to delete written text or numbers Use the F1 F3 keys to select the corresponding action from the info text bar Figure 4 14 The information on the info text bar is updated according to the active menu Info text Protocol1 Protocol2 Protocol 3 bar Function button Figure 4 14 Info text bar of the Multiskan FC user interface In the Main menu the F1 F3 function keys are reserved for ready made protocols Demonstration protocols Demol Demo3 for example single measurement kinetic measurement dual endpoint measurement are set for quick selection F1 F3 keys You can change the protocols for the F1 F3 keys with the FILE key in the protocol list view Press the FILE key for example for saving the active protocol in the Main menu Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol New Save Save as Export Export all protocols Import Print and Delete Use the HELP for viewing more detailed instructions Thermo Fisher Scientific Operating the Internal Software Display and keys for navigating and editing Press the PLATE in out button to move the plate carrier in or out Press the START button to start microplate reading using the currently selected protocol Press the STOP button to terminate the reading
30. process Data from the plate in progress is lost It can also be used to terminate the possible computer remote control Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 27 Operating the Internal Software Display and keys for navigating and editing 28 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Thermo Fisher Scientific Main Protocol Plate format Measurement Shaking Incubation Chapter 5 Menus and Parameters This section describes the menus and the relevant main parameters of the internal software for creating and editing protocols The internal software includes the Main Processing Results and Settings menus The user menu layout is displayed in Table 5 2 Table 5 2 Program overview Processing Results Settings Plate layout Raw data System Preprocessing Calculated results Filters L Calculation Calibration curve Printer Interpretation Quality control L Startup Quality control Results in list format Main menu You can specify the instrument related parameters in the Main menu The Main menu contains the Protocol Plate format Measurement Shaking and Incubation parameters Processing Results Settings Untitled 96 Yells Measurement 405nm 1 reading lt gt Shaking No 1 37 0 C Incubation No OK Edit Demol Demo2 eee lt Info text bar The clock on the Main tab shows the real time The Incubation icon a
31. quality control if failed The results of a failed run are flagged e Interpretation e Quantitative results Precalculated results e Raw data Results Raw data Protocol De10 10 09 2008 13 24 00 Thermo Fisher Scientific At CTRL1 Filter 1 405nm 2 A B C D E F G H 4 gt Move 4 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 5 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 OK Edit 6 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 7 8 9 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 Close 10 1 12 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 1 Press the F3 Menu key to enable menus of different data views in order to view them Thermo Scientific Multiskan FC User Manual 79 Viewing Results Viewing results 2 Select another data view using the Up and Down arrow keys and press the OK button AVE ov E 3 Press the FILE key in the data view to print or export a data view Select Export As Text or Print using the Down arrow key if necessary and then press the OK button 4 Press the F2 key to close the data view 5 Select another row from the Results menu using the Up and Down arrow keys and press the OK button To view different data views or to print refer to Steps 2 4 AVE 6 Press the F2 key to close the data view 7 Press the Left arrow key to return to the Main menu 80 The
32. responsibility whatsoever for system malfunctions arising from power failures About This User Manual Intended users The Multiskan FC as standalone or with SkanIt Software can be used in research and routine test laboratories by professional personnel How to use this This user manual is for the following instruments Multiskan g FC Multiskan FC with incubator Cat no 51119000 51119020 user manual 51119050 51119100 51119120 and 51119150 It has been designed to give you the information you need to e Review safety precautions e Install the Multiskan FC e Navigate and edit in the Multiskan FC user interface e Make a protocol and measure e Perform basic cleaning and maintenance procedures e Troubleshoot the instrument performance This user manual also describes all the features and specifications of the Multiskan FC instrument as well as ordering information Read the manual in its entirety before operating the instrument Keep the user manual for future reference The user manual is an important part of the instrument and should be readily available during use of the instrument Keep the user manual together with the instrument in case you distribute it onwards For more For PC software related issues refer to the Thermo Scientific SkanIt information Software for Multiskan FC User Manual Cat no N07713 For the latest information on products and services visit our websites at http www thermo com http www
33. sample using the Up arrow key and then press the OK button Press the F1 key to accept the selection and return to the Layout window Thermo Fisher Scientific Programming calculations and interpretations Programming precalculations Thermo Fisher Scientific Protocol Creation Examples Programming calculations and interpretations 24 Press the OK button to start filling the plate from well F1 25 Select the Well type item in the Add series starting from well F1 window and press the OK button 26 Select for example an Unknown sample using the Up arrow key and then press the OK button 27 Select the Number of item using the Down arrow key and press the OK button 28 Select for example 91 using the number keys and then press the OK button 29 Press the F1 key to accept the selection and return to the Layout window The filled wells will show the following colors for blank wells green for calibrators bright green for controls and blue for unknowns Processing Well A1 Name BLA Replicate 1 1 00006066GGGS A p Move OK Add Accept Cancel Edit Delete 30 Press the F1 key again to accept the plate layout and to return to the Processing menu In this section examples of programming calculations or interpretations are described Preprocessing carries out calculations that are normally made as a first step in result handling that is preprocessing and kinetic calculations Pre
34. solution 1 3 e Glutaraldehyde solution 4 e Chloramine T e Microcide SQ 1 64 Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde negatively affect the enzyme being used in EIA tests resulting in bad test results A To decontaminate the instrument follow these steps 1 Prepare the decontaminant 2 Empty the plate carrier Ensure that you are wearing disposable gloves 3 Switch OFF the power and disconnect the power supply cable Thermo Fisher Scientific Adding individual filters to the filter wheel A A Thermo Fisher Scientific 10 Maintenance Adding individual filters to the filter wheel Disinfect the outside of the instrument or to remove stains using a cloth dampened with 70 ethanol Place the instrument in a large plastic bag Ensure that the lamp and filter wheel chamber cover and measurement chamber door are open and the plate carrier is out Place a cloth soaked in the prepared decontaminant solution into the bag Ensure that the cloth does not come into contact with the instrument Close the bag firmly and leave the instrument in the bag for at least 24 hours Remove the instrument from the bag After decontamination clean the instrument using a mild detergent After performing this decontamination procedure enclose a signed and dated Certificate of Decontamination both inside the
35. the Main menu shows the name of the active protocol You can open another protocol by pressing the OK button on the Protocol row or by pressing the FILE key The list of protocols saved in the internal software will appear You can also open a particular run of the protocol in question protocol with measured data with the Menu function Also the runtime is shown on the Protocol row For more details on opening a protocol refer to Opening a new protocol on page 65 Processing Results Settings Cell prolif Clamydia Demot1 Demo2 Demo3 Endotoxin Helicobacter HIV 1 2 MTT Created 17 06 2006 13 00 14 17 06 2008 13 01 16 17 06 2008 13 02 02 17 06 2006 13 02 34 17 06 2008 13 02 50 17 06 2008 13 03 02 17 06 2008 13 03 40 17 06 2008 13 04 44 17 06 2008 13 05 30 17 06 2008 13 05 54 Modified 17 06 2008 13 01 16 17 06 2008 13 02 02 17 06 2008 13 02 34 17 06 2008 13 02 50 16 06 2008 09 26 14 17 06 2008 13 03 40 17 06 2008 13 04 44 17 06 2008 13 05 30 17 06 2008 13 05 54 un OK Load a Move The maximum amount of protocols available is 99 Table 5 4 Protocol structure Protocol 1 Runt Run2 Protocol 2 Runt Run2 Run3 A measured run of a protocol includes run data and protocol information for that run You can start the measurement of an active protocol with the name Undefined but the results of an Undefined protocol are not saved unless you save the active protocol
36. the following parameters e Plate layout with well information e Preprocessing for example kinetic calculations e Calculation for example curve fit e Interpretation for example limit calculation e Quality control rules for quality control TIM Results Settings Plate layout Preprocessing Calculation Interpretation Quality control Move OK Edit Demot Demo2 ee e Info text bar See the info text bar for required actions You can set the plate map of the protocol in the Plate layout window The calculations of the results are made based on the plate layout Processing Well A1 Name EMPTY HOOOOOOOOOO0O amp 4p Move OK Add Accept Cancel Thermo Scientific Multiskan FC User Manual 35 Menus and Parameters Processing menu 36 Thermo Scientific Multiskan FC User Manual Table 5 7 Processing parameters Processing Plate layout Plate layout L Well A1 Name EMPTY L Well type L Blank L Calibrator L Control L Unknown L Empty L Fill direction L Down L Right L Replicate direction L Down L Right L Number of Li l Replicates Li Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol You can start filling the plate layout on any of the wells of the plate You start the fill procedure from the well marked with a blue sguare You can Start filling a series of samples by pressing the OK button in the highlig
37. well position X First well location Y Corner distance X Corner distance Y Table 5 6 384 well plate dimensions 384 well plate Plate height Plate length Plate width First well position X First well location Y Corner distance X Corner distance Y Dimension mm 14 35 85 48 127 76 14 38 11 24 99 63 Dimension mm 14 35 85 48 127 76 12 13 8 99 103 5 67 5 Thermo Fisher Scientific Menus and Parameters Main menu Measurement You can define the measurement filter s number of readings and para meters measurement mode in the Measurement window Filters Filter 1 defines the first main filter Filter 2 defines the second reference filter Measurement parameters Filter 1 nm 05 No of readings 1 100 Filter 2 nm No filter Mode Fast 4 gt Move OK Edit Accept Cancel You can select the suitable filter from the list of available filters You must physically install the filter into the filter wheel as well as introduce the additional filter to the internal software of the instrument in the Settings window Refer to Introducing a filter to the internal software on page 89 Measurement modes There are two measurement modes Normal and Fast The measurement head stops on each well in the Normal measurement mode The Normal mode produces the most accurate results with measurement times that are fast enough for most purposes The head moves continuously in the Fast meas
38. y denotes the expected value The minimum number of calibrators for this fit type is two Extrapolation is only available for the linear regression fit type Four parameter logistic The four parameter logistic method uses the fit model below a b b4 1 xc In the equation above y is the signal x the concentration a the response at high asymptote 6 the response at low asymptote c the 1 concentration corresponding to 50 specific binding and d the slope factor The minimum number of calibrators for this fit type is five The Concentration logarithmic transformation is not available for this fit type Extrapolation is not available for the four parameter logistic fit type Cubic spline This method is a smoothed point to point method where the adjacent calibration points are connected together using cubic polynomials see the equation below and optimizing the connecting points as smoothly as possible to avoid sharp angles The results are calculated by first searching for the correct interval and then using a bisectional method to find the answer from the corresponding equation The minimum number of calibrators for this fit type is five Yi Ai bx GX x Extrapolation is not available for the cubic spline fit type Only the means of the measured calibrator replicates can be used not the individual replicate values Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 49 Menus and
39. 0 101 103 104 105 cover 14 17 97 100 101 103 104 105 layout 29 35 36 47 50 54 56 light source 14 17 23 93 97 100 101 102 103 104 105 108 111 112 116 limit 35 44 51 52 73 linear regression 40 48 49 72 linear shaking 107 main parameters 29 30 62 64 65 maintaining a system log 93 105 maintenance 3 62 64 93 94 105 checklist 93 maximum of well 38 40 46 maximum rate 38 40 41 42 43 44 measurement 29 30 31 33 34 40 41 43 45 46 56 65 126 Thermo Scientific Multiskan FC User Manual chamber 19 85 97 105 chamber door 19 85 97 105 mode 33 parameters 33 40 technique 108 time 33 108 measuring 2 12 123 memory stick 18 22 61 62 82 112 menu 25 26 29 35 54 58 layout 29 Multiskan Verification Plate 93 117 navigating 25 normal mode 33 108 123 0 opening a new protocol 31 65 optical 2 17 23 93 94 108 115 123 density 123 system 23 93 94 108 ordering information 3 115 P packing 13 105 106 instructions 105 list 13 materials 106 photometric measurement 3 photometry 108 123 plate 12 107 116 117 format parameters 32 in out switch 27 103 layout 29 35 36 37 47 50 56 57 65 68 71 type 107 point to point 48 50 precalculations 39 40 65 71 72 precision 108 preprocessing parameters 37 38 52 printer 18 29 58 59 61 62 81 82 87 90 112 113 printing 18 29 58
40. 01 108 112 stored curve 47 50 56 switching on 18 symbols 4 system 2 5 12 23 29 43 58 59 87 88 93 94 105 108 13511551195 123 log 105 settings 87 System 29 59 87 105 115 119 system log 93 105 T time to change 38 40 44 time to maximum 38 40 43 46 time to maximum rate 38 40 43 transmittance 123 transport 13 14 15 16 97 103 104 105 107 114 damage 13 package 13 97 troubleshooting 111 type label 4 94 U uniformity 109 USB 18 19 22 61 62 81 82 83 107 112 116 123 memory stick 18 22 61 62 81 82 83 112 memory stick port 22 81 82 83 user interface 3 26 107 V verification tools 117 viewing results 79 W warm up time 109 warning codes 111 113 warranty 13 wavelength 11 23 33 38 39 40 59 65 71 88 89 100 108 123 accuracy 108 range 11 108 Window 38 41 43 44 45 Thermo Scientific Multiskan FC User Manual 127 Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 128 Notes Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 129 Notes 130 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Notes Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 131 Thermo Fisher Scientific Oy Microplate Instrumentation Ratastie 2 RO Box 100 Fl 01621 Vantaa Finland www thermo com N07710
41. 16 accuracy 2 94 108 adding a QC rule 54 75 adding individual filters to the filter wheel 97 application 2 5 12 autoblanking 23 108 autocalibration 23 123 average rate 33 38 39 40 Baseline readings 38 45 C calculated results 29 54 56 79 81 82 calculation parameters 29 31 35 36 38 47 48 51 54 55 68 715 725 79579 calibration 29 49 50 54 56 57 79 112 curve 29 56 57 Change 38 45 93 Change type 38 45 changing the lamp 93 101 102 changing the language 87 cleaning the filters 95 connecting to a computer 18 connecting to a printer 18 cubic spline 48 49 curve fit 35 39 41 47 48 57 72 cutoff 51 52 D decontamination 85 93 95 96 97 105 121 123 Certificate of 97 105 121 procedure 85 93 95 96 97 105 121 detector 23 108 123 disposal of instrument 105 disposal of materials 96 Thermo Fisher Scientific E enabling QC 54 75 endpoint measurement 26 123 environmental requirements 14 error 19 105 111 112 113 123 codes 111 messages 19 105 123 exporting a protocol 82 exporting results or protocols 55 56 57 80 81 82 83 F factor 47 48 49 50 filter wheel 14 16 17 19 23 33 88 89 90 93 97 98 99 100 101 103 104 105 108 111 112 filters 16 19 23 29 33 58 59 87 88 89 93 94 95 97 98 100 101 108 112 115 four parameter logistic 48 49 G glossary 123
42. 96 A Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 105 Maintenance Disposal of the instrument 106 Thermo Scientific Multiskan FC User Manual Warning The used lithium Li battery is regulated waste and must be disposed of according to strict EPA Environmental Protection Agency requirements The Li battery has to be changed by an authorized service technician only Instructions for changing the Li battery are described in the service manual A Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The proposals for the procedures vary by country Pollution degree 2 see Safety specifications on page 109 Method of disposal Electronic waste Contaminated waste Infectious waste WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo Fisher Scientific s compliance with these Directives the recyclers in your country and information on Thermo Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEEROHS aA Regarding the original packaging and packing material
43. Note You can only import the runs to the same instrument they were created with You can also import the protocol to an instrument with the same configuration A 3 Press the FILE key and select mport using the Down arrow key and then press the OK button Select the protocol you want to import from the USB protocol list using the Up or Down arrow key and press the OK button EE 5 Press the F2 key to close the protocol list and return to the Main menu 84 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Chapter 10 Shutdown Shutti ng down To shut down the Multiskan FC follow these steps 1 Remove any plates still in the instrument ape 2 Press the PLATE in out button Make sure the plate carriage goes in out inside the instrument and check that the measurement chamber door closes properly 3 Switch OFF the instrument 4 Ifyou have spilt infectious agents on the instrument disinfect with y 8 70 ethanol or some other disinfectant see Decontamination procedure on page 96 Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 85 Shutdown shutting down 86 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Chapter 11 Modifying Settings You can modify settings related to the system filters printer or startup in the Settings menu Processing i Settings Sea System Filters Printer Startup Move OK Edit Demo1
44. Parameters Processing menu 50 Thermo Scientific Multiskan FC User Manual Factor Stored curve Interpretation parameters Point to point The point to point method connects the adjacent response concentrations coordinates that is the calibration points together using a straight line see the equation below which is different for each of the intervals The results are calculated by first searching for the correct interval and then using the corresponding equation The minimum number of calibrators for this fit type is two N 4 bx Extrapolation is not available for the point to point fit type Only the means of the measured calibrator replicates are used in the calculations The replicates are visible in the graph but they are not used in calculations Measured absorbances are multiplied with a factor given by the user to obtain the concentrations The concentration is calculated according to the following equation Concentration Factor x absorbance You can use a stored curve for an assay a run You can only use a stored curve with the protocol it was created with After the curve has been stored the plate layout may be modified to contain for example only unknowns You can save the curve from the curve window using FILE Save calibrators and load it using FILE Load curve Note The stored curve must be older than the measured run data This means that if a new curve is stored the earlier saved assay run data c
45. Plate square well Immulon 4 HBX square well Immulon Strip Assemblies in frames Flat 1 B Medium Binding 1 x 12 Flat 1 B Medium Binding 2 x 8 Flat 2 HB High Binding 1 x 12 Flat 2 HB High Binding 2 x 8 Flat 4 HBX High Binding Extra 1 x 12 Flat 4 HBX High Binding Extra 2 x 8 Immulon Strips Flat 1 B Medium Binding 1 x 12 Flat 2 HB High Binding 1 x 12 Flat 4 HBX High Binding Extra 1 x 12 Microtiter Breakable Strip Assemblies in frames Flat Universal Binding 1 x 8 Thermo Fisher Scientific Verification Thermo Fisher Scientific tools Cont Code 95029180 95029350 95029100 Ordering Information Verification tools Item Flat Enhanced Binding 1 x 8 Microtiter Solid Strip Assemblies in frames Flat Universal Binding 1 x 8 Flat Enhanced Binding 1 x 8 Table 16 23 Codes for verification tools Code Item Ouantity 24072800 Multiskan Verification Plate 1 Thermo Scientific Multiskan FC User Manual 117 Ordering Information Verification tools 118 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Appendix A System Log Instrument name number User Date Comments PHOTOCOPIABLE Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 119 System Log 120 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Appendix B Certificate of Decontamination Name Address Tel Fax Name Serial no A I confirm t
46. Scientific Thermo Fisher Scientific Menus and Parameters Processing menu Processing Preprocessing kinetic param Average rate Kinetic rate Ignore from beginning Ignore from end Move OK Edit e Kinetic rate Select to view the results in either seconds s or minutes min The Kinetic rate is always calculated per second but if you select per minute the time is automatically converted to minutes after the calculations e Ignore from beginning Ignores a defined number of points readings counted from the first reading e Ignore from end Ignores a defined number of points readings counted from the last reading Maximum rate If the maximum rate is selected the software searches the data for the maximum rate found in each well To obtain the maximum rate a series of linear curve fits will be performed for different segments of the measurement value absorbance vs time curve Figure 5 15 The first segment starts at the first data point within the selected time and measurement range the second segment starts at the second data point and so on until all the data points have been analyzed All the rate calculations are evaluated to determine the maximum rate In other words the LLS fit of m span points are sequentially fitted through each of the data points There will be n m 1 curves produced from this You can specify the number of data points in a segment with the Window setting The
47. an interpretation to the protocol see Programming an interpretation on page 73 Quality control Quality control QC is used to program rules for quality control and parameters thereby ensure that the assay works as expected You can set a maximum of four QC rules per protocol Processing Results Settings Plate layout Preprocessing Calculation Interpretation Quality control OK Edit Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 53 Menus and Parameters Results menu Table 5 11 Processing parameters Quality control Processing Quality control Quality control L Enable quality control L Yes L Rule 1 L Rule 2 L Rule 3 L Rule 4 LNo Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol The rules formulas can be entered with a QC rule editor The editor contains the following items Sample Select the sample to be checked It can be any of the calibrators or controls present in the layout or the blank If the sample has replicates the average value of these is automatically selected for the QC check Both raw and calculated data of a sample can be used as a criteria Operator One of the following can be chosen to be used in the formula CG gt or lt Constant A constant can be entered with the number keys on the keypad Function SD or CV of a sample can be added SD calculates t
48. annot be recalculated against the new curve A You can define the qualitative calculation classification in the Interpretation window fi Processing i Settings Plate layout Preprocessing Calculation Interpretation G Quality control Move OK Edit Demol Demo2 Demo3 Thermo Fisher Scientific Thermo Fisher Scientific Menus and Parameters Processing menu Table 5 10 Processing parameters Interpretation Processing Interpretation Interpretation L Source data L Interpretation 1 L Limit 1 L Sample LBLA L Raw Blanked Dual Kinetic Concentration L CALI CAL12 L Raw Blanked Dual Kinetic Concentration L CTRLI CTRLS5 L Raw Blanked Dual Kinetic Concentration L Operator L L Constant L 1 00 L Function LSD CV L Delete L Interpretation 2 L Limit 2 L Sample LBLA L Raw Blanked Dual Kinetic Concentration L CALI CAL12 L Raw Blanked Dual Kinetic Concentration L CTRLI CTRL5 L Raw Blanked Dual Kinetic Concentration L Operator Leds epi L Constant L 1 00 L Function LSD CV L Delete L Interpretation 3 The pop up menu appears when you double click Sample Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol Interpretation classification can be used in a qualitative assay a run where no absolute concentration is calculated and it can also be used for a quantitative assay a run when the interpretation is made based
49. ansport lock out 4 Keep the transport lock bar and tag Figure 2 5 on page 16 for future relocation or transportation of the instrument Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 15 Installation Installation setups Figure 2 5 Transport lock bar and tag removed Installing the filter To install the filter wheel follow these steps wheel 1 Unpack the filter wheel Figure 2 6 It is delivered in a filter wheel box Check that all filters are clean and undamaged Position slot TS3580 Figure 2 6 Multiskan FC filter wheel Caution Do not touch the filters with your bare hands A Caution When installing the filter wheel do not touch any other mechanical or electronic part A 16 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Installation Installation setups 2 Place the filter wheel into the filter slot so that the filter wheel numbers 1 8 face outwards Figure 2 7 A magnet locking mechanism will automatically lock the wheel in the correct position and the optical filter position sensor will make sure the correct filter is used during measurement Figure 2 7 Placing the Multiskan FC filter wheel into the filter wheel slot If the filter wheel seems to be jumping in the filter wheel slot you have inserted the wheel incorrectly and the magnet is rejecting it Turn the wheel over 3 Close the lamp and filter wheel chamber cover Con
50. aseline The base value is the average of results of the given baseline count from the beginning or end of results Then select whether the change from the base value is evaluated as a relative or an absolute value and define that percentage or value This change is then added to the base value to create a required change value The average of the sliding window is compared to the limit The result is the exact interpolated time where the given change occurs Thermo Fisher Scientific Thermo Fisher Scientific Menus and Parameters Processing menu You can define the following settings i Processing Preprocessing kinetic param Type Time to change Baseline readings 100 Ignore from beginning 0 Readings from Beginning Ignore from end 0 Change 0 00 Window 2 Change type Absolute Reaction Undefined 24 gt Move OK Edit Ignore from beginning Ignores a defined number of points readings counted from the first reading Ignore from end Ignores a defined number of points readings counted from the last reading Window The number of consecutive readings to use for evaluation Reaction Define whether the reaction is supposed to produce increasing or decreasing signal levels There are three options for this setting e Undefined Searches for the defined change in signal regardless of the rate polarity positive or negative and returns the corresponding time e Increasing Searches for the in
51. asuring absorbance from suitable microplates and strips in 96 well plate formats and optionally microplates in 384 well plate formats that meet the ANSI SBS standards The Multiskan FC as standalone instrument or with SkanIt Software can be used in research or routine test laboratories by professional personnel The Multiskan FC is part of an analyzing system for the end user who is thus responsible for validation of the whole system for producing reliable and safe results If the assay performance is essential for the analysis the test result has to be ensured with internal quality controls or with an alternative test It is encouraged to use Good Laboratory Practices GLP during the analyzing process Use for self testing is excluded Caution Operate the instrument only with software and hardware specifically designed or selected for it Thermo Fisher Scientific assumes no liability for the use of third party software applications A Thermo Fisher Scientific A What to do upon delivery How to unpack Checking delivery for completeness or Thermo Fisher Scientific A damage Chapter 2 Installation This chapter describes the installation setups of the Multiskan FC instrument Warning The Multiskan FC weighs 8 5 kg 18 7 lbs and care must be taken when lifting it A This section covers the relevant procedures to be carried out upon arrival of the instrument Move the packed instrument to its site of o
52. bserved with this instrument Warning Do not touch switches or electrical outlets with wet hands Switch the instrument OFF before disconnecting it from the mains supply 4 Defects and This section describes about defects and abnormal stresses abnormal stresses Warning If the instrument is not functioning properly it may create electromagnetic perturbation which could impair the operation of other devices or equipment in the usual environment A Whenever it is likely that the protection has been impaired the instrument should be made inoperative and be secured against any unintended operation Contact authorized technical service immediately The protection is likely to be impaired if for example the instrument e Shows any visible damage e Fails to perform the intended functions e Has been subjected to prolonged storage under unfavorable conditions e Has been subjected to severe transport stresses 114 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific List of filters Thermo Fisher Scientific Chapter 16 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Table 16 20 Codes for filters Code 1423405 1423755 1424055 1424145 1424505 1424925 1425205 1425305 1425405 1425505 1425605 1425705 1425955 1426205 1426305 1426505 1426905 1427405 1427505 Item Optical filter 340 nm Optical filter 375 nm Optical
53. calculation is used with dual two wavelength measurements for example subtracting the reference wavelength from the assay measurement wavelength Thermo Scientific Multiskan FC User Manual 71 Protocol Creation Examples Programming calculations and interpretations Processing No No Interpretation No Quality control Wo OK Edit Down arrow key and press the OK button ox 1 Select the Preprocessing row in the Processing menu using the 2 Select the Precalculation type and press the OK button Select for OK example Meal Mea2 using the Down arrow key and press the OK button Processing Preprocessing parameters Precalculation Kinetic Meal Mea2 Meal Mea Meal Mea2 Mea2 Meal Mea2 Meal Move OK Select Cancel 3 Press the F1 key to accept the precalculation 4 Press the Left arrow key to return to the Main menu Programming To program calculations follow these steps calculations vE OK 2 Select Type and press the OK button vE 72 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific 1 Select the Calculation row in the Processing menu using the Down arrow key and press the OK button 3 Select for example Linear regression as the calculation type calibrator curve fit using the Down arrow key and then press the OK button Protocol Creation Examples Programming calculations and interpretations Processing Calculation param
54. creasing defined change in signal and returns the corresponding time If no increasing rate is found the result is NaN not a number e Decreasing Searches for the decreasing defined change in signal and returns the corresponding time If no decreasing rate is found the result is NaN not a number Baseline readings The Baseline readings parameter is the number of initial readings calculated from the beginning first or end ast of the measurement used for the baseline calculation The baseline can also be zero which means that the comparison will be made against zero In the Baseline readings the software chooses as many measurement points as exist in the baseline and calculates the mean thereof The mean equals the baseline value If ast is selected the change is searched starting from the end of the measurement Readings from There are two options for this setting Beginning default or End Change The change in signal is specified and compared to the baseline Change type The relative change from the baseline in percent or the absolute change The default is Absolute Thermo Scientific Multiskan FC User Manual 45 Menus and Parameters Processing menu 46 Thermo Scientific Multiskan FC User Manual Maximum of well The maximum of well is used to search for the maximum measurement value in each well You can define the following settings fz 3 z 1 Processing 1 Preprocess
55. d commands A Note Do not use the instrument if it appears that it does not function Note The instrument does not verify the logic flow of the received When an error is detected the current operation is terminated After an error it is best to abort the current run and restart from the beginning after the problem is fixed The error Table 15 18 and warning codes Table 15 19 that may appear in SkanIt Software for Multiskan FC are presented below Table 15 18 Error codes reported Code Explanation 0 The command was executed successfully 2 The instrument did not recognize the commana it received 3 The arguments of the received command are not valid 4 Plate positioning error 5 Measure head positioning error 6 Filter wheel positioning error 1 AD converter offset voltage is too high 8 AD converter noise level is too high 9 Lamp failure 10 Non volatile parameters lost 11 Attempt to set the instrument serial number when it already has been set 12 Not enough memory for a new user defined parameter Continued Thermo Fisher Scientific Suggested action Contact the PC software vendor Contact the PC software vendor Check that there is nothing preventing free plate movement Contact service Check that there is nothing preventing free measure head movement Contact service Check that the filter wheel is in place Contact service Contact service Contact service Replace the lamp
56. de the user manual How to pack for To pack the Multiskan FC for service follow the instructions presented below service e Inform about the use of hazardous materials e Remove any microplate before decontamination Also remove the filter wheel and put it into its transportation box Decontaminate the instrument e Refit the transportation lock Refer to Refitting the transport lock on page 103 e Close the lamp and filter wheel chamber cover and the measurement chamber door e Pack the instrument according to the packing instructions e Use the original packaging for shipping e Enclose a dated and signed Certificate of Decontamination see Appendix B both inside and attached to the outside of the package in which you return your instrument or other items e Enclose the return authorization number RGA given by your local Thermo Fisher Scientific representative e Indicate the fault after you have been in touch with your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department Disposal of the Ifthe Multiskan FC is exposed to potentially infectious chemical instrument samples toxic or corrosive chemicals or radioactive chemicals waste management of the complete instrument must be carried out to ensure that there is no risk of contamination A Warning Decontaminate the instrument before disposal Refer to Decontamination procedure on page
57. ds on the combination of measure parameters plate movement parameters number of wavelengths and number of measured wells Make sure you started the timer with a long enough wait time Please note that the cooling of the incubation chamber takes time If you get the warning of Temperature is not changing it means that the incubator chamber is cooling slowly It is possible to Start the run despite of the warning message in case the incubation temperature is not critical for the application This instrument is designed to provide full user protection When correctly installed operated and maintained it will present no hazard to The following recommendations are given for added user safety Electrical Ensure that the power supply cable supplied with the unit is always used If a correct type of mains cable is not provided use only cables certified by the local authorities Thermo Scientific Multiskan FC User Manual 113 Troubleshooting Guide Warnings and cautions The power plug should only be inserted into a socket outlet provided with a protective ground contact Never use an extension cable without a protective ground wire Warning Only authorized technical service personnel are allowed to open the instrument Disconnect the instrument from all voltage sources by disconnecting the power supply cable before opening it A The same precautions applicable when using any electrical equipment should naturally be o
58. e category less than Limit 1 for example Neg Interpretation 2 is a typed name for the sample result in the category of higher than Limit 1 but less than Limit 2 for example Pos Interpretation 3 is a typed name for the sample result in the category of higher than Limit 2 for example High pos The interpretation limits can be entered with an editor The editor contains the following items e Sample You can select a sample to be used for a limit calculation The Sample types can be blank BLA Calibrator CAL1 CAL2 or Control CTRL1 CTRL5 If the sample has replicates the average value of these is automatically selected Both raw and calculated data of a sample can be used as a criteria e Operator One of the following can be chosen to be used in the formula or Thermo Fisher Scientific Menus and Parameters Processing menu e Constant is the numeric value used for the calculation A constant can be entered with the number keys on the keypad e Function can be a standard deviation SD or a coefficient variance percentage CV SD calculates the standard deviation of the sample CV calculates the coefficient of variation for the sample e Delete deletes the created formula digit by digit interpretation 3 g high positive Limit 2 Interpretation 2 g positive Limit 7 Interpretation 1 e g negative Figure 5 17 Interpretation and limits For instructions on how to program
59. er mistallA On nee n EE 19 Installation of SkanIt Software for Multiskan FC oooo 19 Multiskan FG Main Parts nana maa 21 FLORE Me ea BR Nu 21 Baek kn naa n AU 21 AEV IEN D TN an E 22 USB memo sick POr rinri aa E 22 Optical Stelan E OT REN 23 TELO LE a RE RN E E E eee 24 VAIS ENEAS E ENE AAN A AA ET ENE IEE N E TN 24 Internal MenO sean am sn 24 Operating the Internal Software ooo oooooooo oom 25 Display and keys for navigating and editing oo 25 Key PC CON kantin edema BA Ka nek nanda nban DAS 25 Menus and Para Meter aa aka 29 Mn men ea ne oa 29 Protocol nenen BNN NN BB 31 Thermo Scientific Multiskan FC User Manual 7 Contents Plate format parane ter maen 32 Measurement para Sie MA Ne 33 JEEE a aan AAA SA studies 33 Measurement modes Keke tan edukasi skala 33 Shan pa am ee en aa 34 Pee tills on ae en E 34 Processie menu na aa EA 35 PlateslavOut parameter reciia aeni n R T 35 Preprocessing parameter Se aa 37 Presale OS es aeaneenacteectace 39 Kinetic CalculatiOns Mao sada 40 Caloun on parameters ea BA laman ANN BN 47 TV TUS AA AN NAN AN 48 PACT OL Pn AN EN UN PENA TN E PAN 50 0 Lean Enon ne ere ROR An en ee Eo ee mee oN 50 Tnterpretatom parameter en E E 50 SOC Pi NA NE AAA TON AA AN AA AA An 52 Interpreton amd lini Sue RERE B3 52 Quality control parameters sorea EU 53 Reile ae ion O O an BN 54 EEES e E AEAN EE an EE S T EAE EE 55 e EE l E E E E A TE E T 56 Cahibratiom CUVEE anen a a a NOTAE 56
60. ers not ok 50 File open error File not found 51 Filter 1 not selected in measure parameters Continued 112 Thermo Scientific Multiskan FC User Manual Suggested action parameters to make room for new parameters The internal memory is full Take a backup by exporting the protocols runs Refer to Chapter 9 Printing Exporting and Importing Release space from the internal memory by deleting protocols runs from the internal memory When you connect to the instrument with the VER command the instrument should not report any error in the response If it does try to eliminate the error If you are unable to eliminate the error contact Service Check the parameters of the PLA command used Use the MEA command instead of the SCA command Use a shorter sampling time in the MPA command Check that there actually is a filter in all used filter positions If the error persists contact service This error may also be caused by a measure head or plate position error see errors 4 and 5 Contact service Contact service Define at least one filter you are using Contact service Contact service Contact service You should never see this error This is not fatal the instrument still works but command parsing is a bit slower Contact service Contact service Contact service Contact service Contact service Use a at least a 4 C higher incubation temperature than the environmental temperature
61. eters Linear regression Four parameter logistic Cubic spline Point to point Factor Move OK Select Cancel 4 Press the F1 key to accept the selection 5 Press the Left arrow key to return to the Main menu Programming an The following example shows how to add an interpretation to a interpretation Protocol The interpretation limit in this example is the absorbance of the negative control 0 05 Abs The samples below the limit are categorized as negative Neg and the samples above it as positive Pos Down arrow key and press the OK button oK 1 Select the Interpretation row in the Processing menu using the OK 2 Select Source data and press the OK button 4 OK 3 Select for example Blanked using the Down arrow key and then press the OK button Processing Interpretation parameters Interpretation 3 Limit 2 Interpretation 2 Limit 1 Interpretation 1 source data Concentration Move OK Select Cancel button ox 4 Select Interpretation 1 using the Up arrow key and press the OK Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 73 Protocol Creation Examples Programming calculations and interpretations p Eg 3 WXYZ 5 6 Enter the category name for example Neg by using the letter keys and press the OK button Select Limit negative using the Up arrow key and press the OK button The limit editor is opened Process
62. ettings refer to Chapter 11 Modifying Settings ec Startup System OK Edit Demol Demo2 a Move Settings Info text bar Thermo Fisher Scientific Menus and Parameters Settings menu Table 9 12 Settings parameters Settings System L Date L 22 09 2008 14 19 01 L Date format l dd mm yyyy l dd mm yyyy ly dd mm yyyy L yyyy mm dd L mm dd yyyy L Time format L 12 hour L 24 hour L Language L English L Russian L French Ls Spanish L Chinese L Japanese ls Portugese L German Filters L Wavelength L 405 1 L 450 2 L 620 3 L Comment Printer L Printer parameters L Enable header L Yes L No L Header text L For example Laboratory 123 Startup L Plate position L In L Out L Temperature C L No L 25 50 Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 59 Menus and Parameters Settings menu 60 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Starting a ready made protocol with the quick keys F1 F3 A PLATE in out Thermo Fisher Scientific Chapter 6 Starting Ready made Protocols Use the F1 F3 function keys reserved for ready made demo or favorite protocols to measure routine assays quickly Three default protocols can be connected to the F1 F3 keys You can change these protocols from Protocol gt OK gt FILE gt Quick Key gt Set Clear F1 F3 Caution 1 Do not switch the power off during
63. f burns A Warning Risk of injury to the user s A Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application A Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest A DE EEP Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 5 About This User Manual safety symbols and markings 6 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Thermo Fisher Scientific Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Contents aren ae KBR eet er 3 Howto Use this user manual Banda 3 Formore mtor On ka 3 Satery symbolsand markingS islan sunan ame am aa 4 Safety symbols and markings used on the Multiskan FC 4 Warning and other markings used in the documentation 5 Introduction to the Multiskan FG 11 IST ted 60 Cove 1 E nn ce eee PE ee ey ee 12 instal AO aa ea aa ai 13 What t ODOM VCE san mann 13 One Upacara EAO 13 Checking delivery for completeness or damage oo 13 Environmental reguyirements ibn aan hana 14 Insan ea one 14 Releasing rhetransportlOeks oo o moo mmk ena 14 Installing the filter Wheel es aa 16 Connecting the power supply cable ooo oWoo 17 Connecting t a Computer AN NN E 18 Corinecing toa Printer ea en aan 18 SWIAT ON ne 18 Pertorming the operational check meises anna 18 Setungs aft
64. filter 405 nm Optical filter 414 nm Optical filter 450 nm Optical filter 492 nm Optical filter 520 nm Optical filter 530 nm Optical filter 540 nm Optical filter 550 nm Optical filter 560 nm Optical filter 570 nm Optical filter 595 nm Optical filter 620 nm Optical filter 630 nm Optical filter 650 nm Optical filter 690 nm Optical filter 740 nm Optical filter 750 nm Thermo Scientific Multiskan FC User Manual 115 Ordering Information List of spare parts and accessories List of spare parts and accessories List of Thermo Scientific plates 116 Thermo Scientific Multiskan FC User Manual Code NO7710 N07713 5187100 1410101 N04001 Code 9503060 3395 3455 3859 9502227 95029330 95029780 95040000 8999 8 59 6310 6505 6309 6506 6405 6508 6301 6302 6404 95029390 Continued Table 16 21 Codes for spare parts and accessories Item Ouantity Multiskan FC User Manual 1 Skanit Software for Multiskan FC User Manual 1 Skanlt Software for Multiskan FC Research Edition 1 Halogen lamp 6V 10W 1 USB A B device cable 1 longer USB cables available from PC stores Table 16 22 Codes for plates Item MultiFrame Immulon Plates 96 Well Flat 1 B Medium Binding Flat 2 HB High Binding Flat 4 HBX High Binding Extra Microtiter 96 Well Plates Flat Universal Binding Flat Enhanced Binding Flat Universal Binding Sterile Lid Microtiter 384 Well Plates Round 384 Plate Immulon 1 B
65. hat the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B I confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological e Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals PHOTOCOPIABLE Please include decontaminating solution used Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 121 Certificate of Decontamination 122 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Glossary absorbance optical density A logarithmic function of the transmission of a wavelength of light through a liquid log b dimension Ads decontamination Removal or neutralization of radiologic bacteriological chemical or other contamination disinfection The destruction of pathogenic bacteria usually with an antiseptic chemical or disinfectant EIA Enzyme immunoassay An immunoassay using a color changing enzyme substrate system for indicating results A d
66. he standard deviation of the sample CV calculates the coefficient of variation of the sample Delete deletes the created formula digit by digit For instructions on how to add a QC rule to a protocol refer to Adding a QC rule to the protocol on page 75 Results MENU You can view the results of a measured run assay in several formats in the Results menu depending on the protocol settings in the Processing menu raw data in list and table format calculated results in list and table format and graphs calibration and kinetic curves 54 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Thermo Fisher Scientific 1 Raw data Menus and Parameters Results menu F Processing K Results wa Settings Abs Raw data f Abs gt Calculated results Calibration curye Quality control Results in list format gt Move OK Edit Demot Demo2 Demo3 Info text bar The assay data that is linked to the protocol consists of raw data and all the information needed for calculations programmed in the Main and Processing menus The result of the previously run assay protocol measured protocol is shown as default if exists When another protocol is selected from the Main menu the result view is cleared to avoid any conflicts Note The instrument measures the absorbance with four decimals Absorbance data is presented with three decimals However all measured decimals are used in furt
67. her calculations A Note Results over 4 Abs are calculated but they are struckthrough and interpretations for these results are not given A You can see the unprocessed data raw absorbances of the assay in the Raw data window Raw data Protocol De10 10 09 2008 13 24 00 Atl CTRL1 Filter 1 405nm 1 2 3 4 5 6 7 10 1 12 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 B C D E F G H 0 204 0 204 0 204 0 204 amp 4b Move OK Edit You can disable enable samples and sample replicates from the raw data by pressing the OK button Select Disable and press the OK button You can print or export the selected data For further information refer to Chapter 8 Viewing Results Thermo Scientific Multiskan FC User Manual 55 Menus and Parameters Results menu Calculated results Calibration curve 56 Thermo Scientific Multiskan FC User Manual The calculated results are shown in the Calculated window Processing Settings Interpretations Protocol De10 10 09 2008 13 24 00 At CTRL1 Filter 1 405nm 1 2 5 9 10 11 12 AREN Nes Nes Nes Nes Nes Nes Nes 4 gt Move Note Blank subtraction is made automatically if the plate layout includes blanks The blank used in the calculations is always an average of the blanks included in
68. hted well You can edit and delete a single well with the Edit function Well type Fill direction Number of Move OK Select Cancel Note Blank subtraction is made automatically if the plate layout includes blanks The blank used in the calculations is always an average of the blanks included in the layout A You can determine the sample type in Well type The sample types are Blank Calibrator Control Unknown or Empty Thermo Fisher Scientific Menus and Parameters Processing menu Note The results will not be calculated for the Empty sample type A Note The calibrator concentrations are programmed in the Plate layout window You can define calibrator concentrations when you create a calibrator series or activate a single calibrator A calibration concentration of 0 is not accepted A me Fill direction can be either to the right or downwards from the active well in the Plate layout window Fill replicate direction can be either the same or different from the fill samples direction in the Plate layout window You can define the number of samples belonging to the group for example the number of different calibrators or the number of different unknown samples in Number of You define the number of replicates for each sample type in Replicate different controls when using Add series starting from the well programming Use the F3 Edit key to program each sample individually if a different replicate number
69. iagnostic test method to measure or detect a substance using antibody antigen reactions ELISA Abbreviation for enzyme linked immunosorbent assay error message Indication that an error has been detected fast mode The detector moves over the well without stopping filter An optically wavelength selective component initialization tests So called self tests which are carried out before operation to ascertain that the necessary instrument adjustments have been carried out kinetic measurement Measurement over a time period at certain intervals normal mode The detector stops over the measured well optical density absorbance log 1 transmittance log Wo dimension O D photometer A device measuring absorbance or optical density photometry The measurement of the properties of light particularly luminous intensity Thermo Fisher Scientific self tests Initialization tests and adjustments that the instrument performs before operation as well as autocalibration single measurement Synonym to endpoint measurement assay method The measurement is only done with a single reading transmittance The ratio of transmitted and incident light 4 Wlo USB Universal serial bus Thermo Scientific Multiskan FC User Manual 123 Glossary 124 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Index A absorbance 11 12 33 40 41 50 55 73 75 108 123 accessories 13 1
70. ical system components including v optical covers See General on page 94 Clean and check the condition of the filters and the filter wheel position 2x Slot Wipe away spilled saline solutions solvents acids or alkaline solutions v from outer surfaces immediately to prevent damage and wipe with deionized distilled water See Immediate on page 94 If any surfaces have been contaminated with biohazardous material v disinfect with a mild sterilizing solution See General on page 94 Clean the case of the instrument periodically See Cleaning of the v Instrument on page 95 Clean the plate carrier when necessary See Cleaning of the v instrument on page 95 Change the lamp when blown See Changing the lamp on page 101 v Ensure proper shutdown See Chapter 10 Shutdown v v Decontaminate the instrument when relocating the instrument or v sending it for service See Decontamination procedure on page 96 Perform verification with the Multiskan Verification Plate Cat no 2x 24072800 v Service the instrument regularly See Cleaning of the instrument on page 95 and Maintaining a system log on page 105 vV depending on the laboratory conditions and the use and configuration of the instrument Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 93 Maintenance Regular and preventative maintenance General Routine and service procedures must be performed by the user to
71. including the measured run data with a new name Note If you make any changes to the processing parameters of a measured run in the Processing menu the changes are made to that run only If you want to make changes to the protocol and thereby to the future runs you must open the protocol make the changes and press the FILE key Save to save the changes A Note When you press the START button the software always loads the saved protocol regardless of what parameters are active on the display a For instructions on how to start and create protocols refer to Chapter 6 Starting Ready made Protocols and Chapter 7 Protocol Creation Thermo Scientific Multiskan FC User Manual 31 Menus and Parameters Main menu Plate format You can select the plate format 96 or 384 in the Plate format window parameters 32 Thermo Scientific Multiskan FC User Manual Note The 384 format is only available if the instrument version supports 384 well plate measurements A Processing Protocol Plate format Results Untitled 96 Wells Measurement 405nm 1 reading shaking No o 370 C Incubation No OK Edit Demotl Demo2 Demo3 Settings Note Use only plates manufactured according to ANSI SBS standard dimensions A The dimensions of the available plates are given in Table 5 5 and Table 5 6 Table 5 5 96 well plate dimensions 96 well plate Plate height Plate length Plate width First
72. ing Interpretation parameters 4 gt Move OK Edit Accept Cancel oK 7 Select Sample and press the OK button vE 8 ad gt E D BE z wxyz a 74 Thermo Scientific Multiskan FC User Manual Select CTRL 1 by using the Down arrow key and press the OK button Select Raw and press the OK button Select Operator using the Right arrow key and press the OK button Select and press the OK button Select Constant using the Right arrow key and press the OK button Enter the constant for example 0 050 using the number keys and press the OK button Press the F1 key to accept the formula Select Interpretation 2 using the Up arrow key and press the OK button Thermo Fisher Scientific i wee OK WXYZ to y y Va Adding a QC rule to the protocol Thermo Fisher Scientific Protocol Creation Examples Adding a OC rule to the protocol 16 Enter the category name for example Pos positive using the letter keys and then press the OK button 17 Press the F1 key to accept the interpretation Processing Interpretation parameters Interpretation 3 Limit 2 Interpretation 2 Limit 1 Raw CTRL1 0 050 Interpretation 1 Neg Source matrix Move OK Edit Accept Cancel The rules are normally given in the kit insert and can read for example the absorbance of Blank has to be smaller than 0 100 Abs In this case the rules to
73. ing kinetic param Maximum of we Ignore from beginning Ignore from end Move OK Edit e Ignore from beginning Ignores a defined number of points readings counted from the first reading e Ignore from end Ignores a defined number of points readings counted from the last reading Time to maximum The time to maximum is used to calculate the time elapsed before the maximum measurement signal in each well is reached You can define the following settings A Processing y Preprocessing kinetic param Time to maximum Ignore from beginning Ignore from end Move OK Edit e Ignore from beginning Ignores a defined number of points readings counted from the first reading e Ignore from end Ignores a defined number of points readings counted from the last reading Thermo Fisher Scientific Thermo Fisher Scientific Calculation parameters E Menus and Parameters Processing menu You can define the calculation parameters in the Calculation window The available calculation types are curve fits and factor You can select different calibrator curve fit types for quantitative calculation or a factor You can also use a stored curve for quantitative calculation Processing Calculation parameters No Linear regression Four parameter logistic Cubic spline Point to point Factor Move OK Select Cancel Note Blank subtraction is made automatically if the plate layo
74. kan FC User Manual 43 Menus and Parameters Processing menu 44 Thermo Scientific Multiskan FC User Manual You can define the following settings Processing Preprocessing kinetic param Ignore from beginning 0 Ignore from end 0 Window 2 Reaction Undefined 4 gt Move OK Edit Accept Cancel e Reaction Define whether the reaction is supposed to produce increasing or decreasing signal levels There are three options for this setting Undefined Searches for the absolute maximum rate and returns the corresponding time Increasing Searches for the maximum increasing rate and returns the corresponding time If no increasing rate is found the result is NaN not a number e Decreasing Searches for the maximum decreasing rate and returns the corresponding time If no decreasing rate is found the result is NaN not a number e Ignore from beginning Ignores a defined number of points readings counted from the first reading e Ignore from end Ignores a defined number of points readings counted from the last reading e Window The number of consecutive readings to use for evaluation Time to change The time to change is used for calculating the time required to reach a defined change in the signal in each well The time is given in seconds or minutes from the first reading First define the base value by entering the number of readings from the start in b
75. lect the Cal 1 item using the Down arrow key and press the OK button Thermo Scientific Multiskan FC User Manual 69 Protocol Creation Examples Programming a plate layout p EA Os to wxyz D Bgn 7 WxyZ Ea OK WXYZ F r A F 70 Thermo Scientific Multiskan FC User Manual Enter for example 7 1 00 as the Cal 1 concentration using the number keys and then press the OK button Select the Cal 2 item using the Down arrow key and press the OK button Enter for example 2 2 00 as the Cal 2 concentration using the number keys and then press the OK button Select the Cal 3 item using the Down arrow key and press the OK button Enter for example 3 3 00 as the Cal 3 concentration using the number keys and then press the OK button fh Processing Concentrations Unit Cal 1 1000 Cal 5 0 000 Cal 9 Cal 2 2000 Cal 6 0 000 Cal 10 Cal 4 0 000 Cals 0 000 Cal 12 Move OK Edit Accept Cancel 18 19 20 2d VIE 2 Press the F1 key to accept the concentrations and return to the previous view i e the Add series starting from well B1 window Press the F1 key to accept the concentrations and to return to the Layout window Press the OK button to start filling the plate from well E1 Select the Well type item in the Add series starting from well E1 window and press the OK button Select for example a Control
76. libration curve window You can print or export the selected data For further information refer to Chapter 8 Viewing Results Quality control QC You can view the quality control data in the Quality control window If the QC rules are not fulfilled and the assay is therefore not valid the QC view is not opened until after the measurement Result Quality control Rule Result 1 RawfBLA lt 0 080 0 0040 0 08 PASS 2 ConciCTRLI gt 20 11 2 FAIL J 4 More You can print or export the selected data For further information refer to Chapter 8 Viewing Results Results in list format The assay results can be viewed in list format in the Results in list format window Results Results as list 405nm Interpretation Abs 0 204 Neg 0 204 Neg 0 204 Neg 0 204 0 000 0 025 405nm Interpretation Close Next page You can print or export the selected data For further information refer to Chapter 8 Viewing Results Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 57 Menus and Parameters settings menu Setting S MENU You can set the instrument parameters in the Settings menu 58 Thermo Scientific Multiskan FC User Manual The values shown in the Settings menu remain in the instrument memory and are instrument specific not protocol specific You can define System Filters Printer and Startup settings For instructions on how to modify different s
77. lso shows the current temperature only Multiskan FC with incubator model See the info text bar for the required actions of the F1 F3 keys The action text on the info text bar changes according to the level of the internal software A detailed program overview of the Main menu is shown in Table 5 3 Thermo Scientific Multiskan FC User Manual 29 Menus and Parameters Main menu Note The parameters of the Main menu are locked when the run measured data for the protocol exists After the measurement the plate format measurement shaking and incubation parameters cannot be changed For more information refer to Chapter 6 Starting Ready made Protocols A Table 5 3 Main parameters Protocol L Protocol Created Modified Runs Plate format L 96 Wells L 384 Wells Measurement L Filter 1 nm L No filter L 405 1 L 450 2 L 620 3 L Filter 2 nm L No filter L 405 1 L 450 2 L 620 3 L Mode L Fast L Normal L No of readings 1 100 Li Shaking L Mode L No L Before measurement L Background Incubation L Temperature C L No L 25 50 L Wait for warm up L Yes L No L Time hh mm ss L 00 00 00 Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol 30 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Thermo Fisher Scientific Protocol Menus and Parameters Main menu The Protocol row in
78. may be very hot Wait for the lamp to cool down before replacing it A or electronic part A Caution When handling the lamp do not touch any other mechanical 3 Turn aside the two clamps which keep the lamp in place Figure 13 24 on page 102 and Figure 13 25 on page 102 4 Lift the lamp up with the terminal socket 5 Loosen the two screws of the terminal socket and pull the lamp from the terminal socket Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 101 Maintenance Changing the lamp amp z 4 Figure 13 25 Lamp changed Caution Do not touch the reflective surface of the new lamp or the bulb itself A 6 Refit the terminal socket to the contacts of the new lamp approved by the manufacturer OSRAM 64222 6V 10W 7 Tighten the screws of the terminal socket and put the new lamp in place 102 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Maintenance Refitting the transport lock Note Place the lamp and terminal socket into the correct position 1 Refer to Figure 13 25 on page 102 a 8 9 Turn the two clamps in place to lock the lamp into position Close the lamp and filter wheel chamber cover 10 Plug in the instrument and switch the power ON Refitting the When you relocate the instrument or ship it for service make sure you transport lock refit the transport lock To refit the transport lock follow these steps l
79. necting the To connect the power supply cable follow these steps power supply cable Warning Never operate your instrument from a power outlet that has N no ground connection Never use a power supply cable other than the Thermo Scientific power supply cable designed for your region A 1 Ensure that the ON OFF switch Figure 2 8 on page 18 at the back of the instrument is in the OFF O position 2 Connect the power supply to a correctly installed line power outlet that has a protective conductor that is grounded 3 Connect the power supply cable to the power input socket Figure 2 8 on page 18 at the back of the instrument Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 17 Installation Connecting to a computer P t USB port for printer USB port for PC P E ee E an ocu USB port for memory stick 7 RA USB USB POWER PRINTER PC 24V DC Figure 2 8 Connecting the power supply cable Connecting tO a Ifyou are using a computer with the Multiskan FC connect the communication cable to the USB port for the computer Figure 2 8 computer P A a Connecting toa Ifyou are using a printer connect it to the USB port for the printer Figure 2 8 The external USB printer protocol that can be used is printer ppcis Switchin ON Check that all the cables are properly fitted according to the installation properly g instructions Switch the instrument ON Caution 1 Do not swi
80. nn Gwe aoe nate ier ten ee erat 87 Tiare rine the Anp Ae es cea cleat care re rae aioe ele N 87 ne an 88 Introducing a filter to the internal software oo 89 Remon AT ter ia ata tan ARA 90 an an NN AN GA NG PAN 90 SN UU NN na AN E SN EA 90 Emergency Situations ooooooo oo oo oo oom 91 Handling emergency situations oooo W 91 EVV AEST ANN NA AAA 93 Regular and preventative maintenance La mena 93 Maintenance cheda Se na AB 93 ST Nan NA A E 94 Kanan tea KN KN a NN ANN aan AN PA NA 94 Cea 11 A eni tai aa aa A aan 95 Cleaning of thie Tastee te ea an en aa 95 Disposak 0rTiateraSu ebeg 96 Decontamination prOcedlitek annisa intan BIN EA 96 Adding individual filters to the filter wheel ooo 97 Chaneine the mp nent c eter near Rr gtr see waren nn trees 101 Retittitie the transport lock saus 103 Mantan aS y Stein IOS uan ba ea 105 FLOW topick TOR SEA Beta me Rene 105 Disposak Ore instrutneht senen ses a Ra AR EN 105 Technical Specifications oooooo ooooo oo ox 107 General Special On Rn Bum 107 Pertormance specifications aan ea RE 108 Sate S erna One na aa ee 109 In conformity with the reguirements ooooo 109 Troubleshooting Guide oooooo oo mena 111 Errorand Warminto cod esteem maha 111 Warninos and canjttOnSu en E T E 113 ra ata 113 Defects and abnormal stresses oo oooooWooWooo 114 Thermo Scientific Multiskan FC User Manual 9
81. nstalled filter 6 Write down in Table 13 14 the additional filters you have installed 9 into the filter wheel It is recommended to add the filters in ascending wavelength order Place the filter spring into its original position and fasten the four screws symmetrically Slide the filter wheel back into the filter wheel slot with the filter numbers 1 8 facing outwards Figure 2 7 on page 17 The magnet locking mechanism will automatically lock the wheel in the correct position Refer to Installing the filter wheel on page 16 Close the lamp and filter wheel chamber cover 10 Switch the instrument ON Thermo Fisher Scientific Maintenance Changing the lamp 11 Add the filters to the internal software s general instrument parameters These parameters are set in the Settings menu Refer to Settings menu on page 58 and Table 13 14 o Note The measurement results will be incorrect if the filter parameters 9 differ from the actual filters in the filter wheel A Now the filters are ready for use Changing the lamp To replace the lamp that has burnt out follow these steps Caution Only use the lamp approved by the supplier Cat no 1410101 Lamp guartz halogen lamp Osram 64222 6V 10W 4 1 Turn the power OFF and unplug the instrument 2 Open the lamp and filter wheel chamber cover Warning If the instrument has been in use and you need to replace a burned lamp the lamp and its surroundings
82. on the calculated concentration In a qualitative classification analysis interpretation the results are divided into separate categories The data is categorized using cutoff values Limit 1 and Limit 2 for up to three different categories Thermo Scientific Multiskan FC User Manual 51 Menus and Parameters Processing menu Source data Interpretation and limits 52 Thermo Scientific Multiskan FC User Manual The limits cutoff values can be set as a number a formula a specified sample or a formula containing both samples and numbers The absorbances or concentrations of the samples are compared to the limits and defined for example as negative or positive You must define the Source data for the interpretation calculation The source data used for interpretation can include the following data types e Raw absorbances Raw e Blank subtracted absorbances Blank e Precalculated absorbances Dual e Preprocessed kinetic values Kinetic e Calculated concentrations For further information refer to Preprocessing parameters on page 37 Processing Interpretation parameters Interpretation 3 Limit 2 Interpretation 2 Pos Limit 1 Raw CTRL1 0 050 Interpretation 1 Neg Source data Move OK Edit Accept Cancel An interpretation calculation always contains at least one limit and two categories Refer to Figure 5 17 on page 53 Interpretation 1 is a typed name for the sample result in th
83. open a new protocol how to program main parameters wavelength shaking incubation the plate layout and precalculation calculation and interpretation parameters and how to save the protocol Processing Results Settings Protocol Untitled amp Plate format 96 Wells 3 Measurement 405nm 1 reading D gt Shaking No J szoc Incubation No Mo OK Edit Demo1 Demo2 Demo3 Saving a new active protocol on page 76 A Note Ensure that you save the changes you make in each step Refer to Opening anew To open a new protocol follow these steps protocol Fite 1 Press the FILE key in the Main menu OK 2 Select New and press the OK button Prog ramming a To program a wavelength follow these steps wavelength Vv oK 1 Select the Measurement row in the Main menu using the Down arrow key and press the OK button 2 Press the OK button at the Filter 1 nm item and select for OK VW OK example 450 as the filter 1 value using the Down arrow key and then press the OK button Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 65 Protocol Creation Examples Programming shaking Programming shaking va sw Cs vV E vE BE o wxyz Programming incubation 66 Thermo Scientific Multiskan FC User Manual 3 Press the F1 key to accept the selection and return to the Main menu To program the shaking parameters follow these steps
84. peration To prevent condensation the instrument should be left in its protective plastic wrapping until the ambient temperature has been reached Unpack the Multiskan FC instrument and accessories carefully with the arrows on the transport package pointing upwards Place the instrument onto a laboratory bench Caution Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so may cause misalignment and will void the instrument warranty A Retain the original packaging for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use If you relocate your instrument or ship it for service refer to How to pack for service on page 105 Check the enclosed packing list against order Visually inspect the transport package the instrument and the accessories for any possible transport damage If any parts are missing or damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy Thermo Scientific Multiskan FC User Manual 13 Installation Installation setups Environmental When you set up your Multiskan FC avoid sites of operation with requirements excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture o
85. prevent unnecessary wear or hazards and are described below at the frequency with which they should be applied Always ensure that the electrical supply in the laboratory conforms to that specified on the type label of the instrument To guarantee the continuous reliability and accuracy of the Multiskan FC avoid disturbing any of the optical system components A misalignment of the light path affects measurements e Keep the filters clean to ensure proper function and accurate results e Prevent any liquid from entering the instrument e Keep the instrument free of dust and other foreign matter e Avoid touching the lens surfaces filters or detectors with your bare hands e Perform the operational check regularly see Performing the operational check on page 18 In the event of any damage contact your local Thermo Fisher Scientific representative for service Abrasive cleaning agents are not recommended because they are likely to damage the paint finish It is recommended that you clean the case of the instrument periodically to maintain its good appearance see Cleaning of the instrument on page 95 Clean the keypad surface with a mild laboratory detergent Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used A Caution Do not autoclave any part of this
86. r large temperature fluctuations Make sure e the working area is flat dry clean and vibration proof and leave additional room for cables covers and so on e the ambient air is clean and free of corrosive vapors smoke and dust e the ambient temperature range is between 10 C 50 F and 40 C 104 F e the humidity is low so that condensing does not occur relative humidity is between 10 and 80 non condensing potentially damaging liquids or gases are present A Caution Do not operate the instrument in an environment where Installation setu pS This section describes the installation setups that have to be carried out before instrument operation Releasing the The instrument comes with a transport lock transport lock Caution Make sure the transport lock has been released before you put the instrument into operation A To release the transport lock follow these steps 1 Open the lamp and filter wheel chamber cover Figure 2 2 t AUTION t Lock ove Transpor Rem B Fa Use Figure 2 2 Transport lock inside the instrument and transport lock tag 14 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Installation Installation setups 2 Unfasten the transport lock bar counterclockwise using the Allen key supplied Figure 2 3 Figure 2 3 Unfastening the transport lock 3 Pull the transport lock bar out Figure 2 4 Figure 2 4 Pulling the tr
87. r warm up is set to Yes the assay will not start until the temperature is reached If Wait for warm up is set to Vo the assay will start a Select the Time hh mm ss item using the Down arrow key and press the OK button 5 Select for example 5 min 00 05 00 as the incubation time by using the number keys and then press the OK button Incubation parameters Temperature C Wait for warm up Temperature Time hh mm ss Move OK Edit Accept Cancel a 6 Press the F1 key to accept the incubation parameters F1 Thermo Scientific Multiskan FC User Manual 67 Protocol Creation Examples Programming a plate layout Programming a plate layout 68 Thermo Scientific Multiskan FC User Manual You can start filling the plate layout on any of the wells of the plate You start the fill procedure from the well marked with a blue square You can start filling a series of samples by pressing the OK button in the highlighted well You can edit and delete a single well with the Edit function In this example e ablank sample e Calibrator 1 Conc 1 e Calibrator 2 Conc 2 e Calibrator 3 Conc 3 e acontrol sample and e 91 unknown samples are programmed To create the plate layout of this example protocol follow these steps 1 Select the Plate layout row in the Processing menu using the Right arrow key Then press the OK button to open the Layout window D gt Well A1 Name EMPTY Co 0 wu
88. rements This will lengthen the lifetime of the lamp Detector and preamplifier Measurement signal Opening for blank reading Background reading point Microwell Plate carrier Fiber guide Light source Figure 3 12 Optical system Autocalibration The lamp intensity in the Multiskan FC instrument is regulated to be suitable for each filter during startup This adjustment procedure is called autocalibration Thermo Scientific Multiskan FC User Manual 23 Multiskan FC Main Parts Incubator Incubator The optional incubator is only available with the Multiskan FC with incubator model The incubator chamber consists of thermal elements on the top and bottom of the incubator chamber The upper electrical thermal element prevents condensation on the microplate that is being incubated Background incubation is also possible during kinetic measurements The incubation temperature ranges from 25 to 50 C max Warning The incubator thermal plates may be hot A Shaker The linear shaker operates at three different speeds Table 3 1 Table 3 1 Shaking speeds Speed name Speed Slow 5 Hz amplitude 15 mm Medium 11 Hz amplitude 3 mm Fast 20 Hz amplitude 1 mm Background shaking is also possible during kinetic measurements Internal memory There is memory for 99 assay protocols and at least assay results for 100 microplates 24 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Chap
89. rmo Scientific Multiskan FC User Manual 41 Menus and Parameters Processing menu Time s Figure 5 15 Determining the maximum rate with window value 2 You can define the following settings Preprocessing kinetic param Maximum rate Reaction Undefined Kinetic rate Ignore from beginning Ignore from end Window W Move OK Edit e Reaction Define whether the reaction is supposed to produce increasing or decreasing signal levels There are three options for this setting e Undefined default The reaction can be increasing decreasing or both for example first increase and then decrease that is peaking The software does not produce any warnings in either case It searches for the absolute maximum rate If the highest absolute rate value is increasing it is shown as a positive number If the maximum Cc rate is decreasing it will be presented with the prefix e Increasing The software searches for the maximum increasing rate The rate is shown as a positive number If no increasing rate is found the result is NaN not a number e Decreasing The software searches for the maximum decreasing rate The rate is shown as a positive number If no decreasing rate is found the result is NaN not a number 42 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Menus and Parameters Processing menu e Kinetic rate Select to view the results in either second
90. rmo Scientific Multiskan FC User Manual Thermo Fisher Scientific Chapter 9 Printing Exporting and Importing The calculated results or stored protocols can be printed after the measurement using the FILE key The printout is sent to the external printer according to the general settings of the instrument The printed data can be selected The report to be printed can be defined by FILE Print Define Report in the main level of Results Each active data table can also be defined by using FILE Print import fails A Note It is recommended to format the USB memory stick if export or Printing Or This section provides information on how the active run data measured exp orting data results can be printed or exported to the USB memory stick Follow these steps 1 Ifyou are exporting data insert the USB memory stick into the USB memory stick port of the instrument OR If you are printing data ensure that the printer is connected and switched on In the Main menu press the Right arrow key continuously till you get to the Results menu Processing A tate Settings Abs Raw data f Abs gt Calculated results Results in list format Move OK Edit Demo Demo2 Fite 3 Press the FILE key Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 81 Printing Exporting and Importing Printing or exporting data vE Exporting a protocol 82 Thermo Scientific Multiskan FC User Manual
91. s s or minutes min The kinetic rate is always calculated per second but if you select per minute the time is automatically converted to minutes after the calculations e Ignore from beginning Ignores a defined number of points readings counted from the first reading e Ignore from end Ignores a defined number of points readings counted from the last reading e Window Select the number of consecutive readings to use for evaluation The highest reaction rate for each well is calculated using a sliding window A window defines how many measurement points are included in the measurement calculations The size of this window is given in the Window parameter box For example if the number of measurements is ten and the Window parameter is three the system will calculate the first rate using the measurements to 3 the second rate using the measurements 2 to 4 and so on up to measurements 8 to 10 The maximum rate will be the maximum value among these calculated rates Time to maximum rate The time to maximum rate is calculated similarly to the maximum rate but the result is reported as the time in seconds from the first reading to the point on the line where the maximum rate occurs instead of the rate itself Refer to Figure 5 16 on page 43 1 4 1 2 1 0 6 _ ol a ee Abs Time fs Time to Max Rate Figure 5 16 Determining the time to maximum rate Thermo Fisher Scientific Thermo Scientific Multis
92. s use the recycling operators known to you For more information contact your local Thermo Fisher Scientific representative Thermo Fisher Scientific General specifications Thermo Fisher Scientific Chapter 14 Technical Specifications Thermo Fisher Scientific reserves the right to change any specifications without prior notice as part of our continuous product development program Table 14 15 General specifications General specifications Overall dimensions Weight Operating conditions Transportation conditions Storage conditions Mains power supply Power consumption Heat dissipation Display Keypad User interface Computer interface Plate types shaker ca 290 mm W x 400 mm D x 210 mm H 11 4 W x 15 7 D x 8 3 H 8 5 kg 18 7 lbs 10 C to 40 C maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Indoor use only 40 C to 70 C packed in transport packaging 25 C to 50 C packed in transport packaging 100 240 Vac 50 60 Hz nominal 100 VA max standby 8 VA 341 BTU max High contrast color display with 480 x 272 dots Four arrow keys OK button three function keys F1 F3 FILE and HELP keys 0 9 number keys a z letters with the number keys C key START STOP and PLATE in out buttons The instrument can be under PC control and run on Skanit Software for Multiskan FC which controls all the instrument
93. shown in Figure 3 11 Lamp and filter wheel chamber cover open Plate carrier OUT Figure 3 11 Multiskan FC side view USB memory The instrument is equipped with a USB port for an external memory stick p ort stick Figure 2 8 on page 18 You can transfer measurement data to the PC and assay protocols from an instrument to another with the USB memory stick 22 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Optical system Thermo Fisher Scientific Multiskan FC Main Parts Optical system The optical system of the Multiskan FC instrument comprises the following components Figure 3 12 Light source The light source is a quartz halogen lamp Osram 64222 6V 10W The lamp is normally off between measurements to prolong the service life the lamp switches on for the measurement Interference filters The filter is a special interference filter The wavelength is selected from one to eight filters held in the filter wheel Fiber The fiber is flexible quartz fiber Fiber end optics Fiber end optics produces a highly focused light beam which passes through the well to the detector Detector housing The detector housing consists of a silicon photodetector an amplifier and lens optics Signal processing The measurement electronics employed has very low noise and a wide dynamic range Autoblanking The dynamic autoblanking procedure ensures that the lamp can be off between measu
94. tch the power off during Performing self diagnostics 2 Do not plug unplug the printer cable during Importing Exporting data from to the USB memory stick 3 Do not plug unplug the USB memory stick during Printing or Performing self diagnostics A Perfo rming the Before you take the instrument into use make time to perform the operational wins operational check check Switch the instrument ON 2 Check that the plate carrier comes out from the instrument and the Self diagnostics passed text is shown for a while on the display 18 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Settings after installation Installation of Skanit Software for Multiskan FC Thermo Fisher Scientific K lt Installation settings after installation 3 Press the START button twice 4 Check that the plate carrier moves in and the measurement chamber door closes properly The instrument starts the measurement After the measurement the plate carrier comes out No error messages should appear on the display 5 Press the F2 key to close the result table 6 Press the Left arrow key twice to return to the Main menu If you want to change the language or add additional filters to the filter wheel modify the required settings in the Settings menu Refer to the instructions in Settings menu on page 58 For installing SkanIt Software for Multiskan FC refer to the Skanlt Soft
95. ter 4 Operating the Internal Software This section describes the display and keys on the keypad for navigating and editing the internal software of the Multiskan FC instrument Display and keys The keypad and display are shown in Figure 4 13 for navigating and editing SCIENTIFIC MULTISKAN FC Menu Plate format Uk ells Display Gs Measurement 405nm 1 reading Shaking Info text bar Function keys in out Number keys with letters Arrow keys and OK button START STOP and PLATE in out control buttons Figure 4 13 Keypad and display of the Multiskan FC Keys Action The relevant keys for navigating and editing are described in detail below The keys also have other functions depending on the level in the internal software The active row is colored blue Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 25 Operating the Internal Software Display and keys for navigating and editing q4PAYV ox Sel p gt L 4 26 Thermo Scientific Multiskan FC User Manual Use the Left Right Up and Down arrow keys to navigate You can speed up the selection by holding down the arrow key continuously Use the OK button to select and edit the highlighted item It is mostly used for setting and accepting the active parameter Use the character keys to enter numerical data and text e SPACE CHARACTER 5 character code which prints a space e PERIOD decimal point e PLUS SIGN 4
96. the layout A Note The results of precalculations are not available in the data view They are only available in exported or printed reports A The kinetic timing is relative This means that each time point is calculated from the first measurement point with the filter You can print or export the selected data For further information refer to Chapter 8 Viewing Results The calibration curve is shown in the Calibration curve window 25 Abs Calibrators from Plate 19 09 2008 09 46 32 Fit Linear regression Abs 0 0173 0 9711 Cone R2 0 9999 Cone mM OK Edit iban Manu The report also includes the calculated fit parameters of the curve You can use both the curve on the plate and a previously created calibration curve of the same assay protocol You can store a curve for future use with the FILE function You can load a saved curve for the active protocol with the FILE function Ifa stored curve is in use for calculation the calibrators measured with the current plate can be taken into use with the Menu function Thermo Fisher Scientific Menus and Parameters Results menu Note The plate layout must include calibrators to enable them for further calculations for example curve fits Refer to Curve fits on page 48 A rn Note Verify the correctness of the calibration curve A ms You can disable and enable calibrator replicates from the curve by pressing the OK button in the Ca
97. tic L Type L No L Average rate L Kinetic rate L s L min Es Ignore from beginning Lo Ls Ignore from end Lo L Maximum rate L Kinetic rate L s L min L Ignore from beginning Lo l Ignore from end Lo L Window L2 L Reaction L Undefined ls Increasing L Decreasing L Time to maximum rate L Kinetic rate L s L min ky Ignore from beginning Lo ks Ignore from end Continued Thermo Fisher Scientific Precalculations Thermo Fisher Scientific Menus and Parameters Processing menu Cont g Processing Preprocessing Lo L Window L2 L Reaction L Undefined L Increasing 3 Decreasing L Time to change Ls Ignore from beginning Lo L Ignore from end Lo L Window L2 L Reaction L Undefined ks Increasing L Decreasing L Baseline readings L 100 Ls Readings from b Beginning L End R Change L 0 00 Ls Change type L Absolute L Relative L Maximum of well Ls Ignore from beginning Lo ls Ignore from end Lo L Time to maximum L Ignore from beginning Lo ine Ignore from end Lo Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol Precalculation is used in the context of dual two wavelength measurements when Filter 1 main filter and Filter 2 reference filter are defined in the Main menu The purpose of the precalculation is to merge the data from the two measurements into one data set which can then be further processed for example using
98. tocol can be transferred from an instrument and imported to another instrument To export a protocol follow these steps Thermo Fisher Scientific Printing Exporting and Importing Printing or exporting data 1 Insert the USB memory stick into the USB memory stick port of the instrument Press the OK button on the Protocol row in the Main menu OR Press the FILE key and select Open using the Down arrow key and press the OK button Select the protocol you want to export from the protocol list using the Up or Down arrow key Move OK Select Cancel 4 Press the FILE key and select Export using the Down arrow key Then press the OK button 5 If you also want to export the runs that are created measured with the protocol press the OK button If not press the Right arrow key and then the OK button E gt Protocol information will be exported 7 Va 6 Press the F2 key to close the protocol list and return to the Main menu Importing a protocol To import a protocol follow these steps 1 Insert the USB memory stick into the USB memory stick port of the instrument Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 83 Printing Exporting and Importing Printing or exporting data ox 2 Press the OK button on the Protocol row in the Main menu OR vV ox Press the FILE key in the Main menu and select Open using the Fite Down arrow key Then press the OK button av E
99. transport package and attached to the outside of the package This section explains how to replace a filter in the filter wheel or add an additional filter to the filter wheel Caution Only use filters approved by the instrument manufacturer A Note The filter is a consumable and should be changed regularly A l 2 Switch the power OFF Open the lamp and filter wheel chamber cover Caution When handling the filter wheel do not touch any other mechanical or electronic part A 3 Lift the filter wheel from the filter wheel slot Figure 2 7 on page 17 and Figure 13 19 on page 98 Do not touch the filter surfaces Thermo Scientific Multiskan FC User Manual 97 Maintenance Adding individual filters to the filter wheel Ly Figure 13 19 Removing the filter wheel 4 Remove the filter spring by unscrewing the four 4 spring position holding screws Figure 13 20 and Figure 13 21 on page 99 Caution Do not touch the filter glass surfaces with your bare hands A Caution The magnet in the middle of the filter wheel attracts both the screwdriver and the screws Make sure the screwdriver or the screws do not scratch the filters A gt D Figure 13 20 Removing the spring position holding screws 98 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Maintenance Adding individual filters to the filter wheel Filter spring Filter Filter wheel Next free position for additional
100. ubation time and temperature in the Incubation window Incubation parameters Temperature C Wait for warm up Temperature Time hh mm ss 00 00 00 Move OK Edit Accept Cancel Incubation is only active if the instrument is eguipped with an incubator Therefore the Incubation icon and row is only visible in the Main menu of the instruments with an incubator indicating the present temperature The temperature range is from 25 to 50 C Refer to Table 14 17 on page 109 If Wait for warm up is set to Yes the assay incubation is not started until the temperature is reached Please note that the cooling of the incubation chamber takes time If you get the warning of Temperature is not changing it means that the incubator chamber is cooling slowly It is possible to start the run Thermo Fisher Scientific Thermo Fisher Scientific Processing menu Plate layout parameters Menus and Parameters Processing menu despite of the warning message in case the incubation temperature is not critical for the application The limits for the incubation time are 00 00 00 99 59 59 The temperature in Kelvin is calculated according to the following equation K t 273 16 where K denotes Kelvin and the temperature in degrees Celsius C You can specify the calculation parameters in the Processing menu These parameters supplement the parameters programmed in the Main menu The Processing menu contains
101. uch as dust occurs with the exception of occasional conductivity caused by condensation A EMC performance EN 61000 6 3 2001 Generic standards Emission standard for residential commercial and light industrial environments EN 61000 6 1 2007 Generic standards Immunity standard for residential commercial and light industrial environments EN 61326 1 2006 Product family standard Electrical equipment for measurement control and laboratory use EN 61326 2 6 2006 Product family standard Electrical equipment for measurement control and laboratory use In vitro diagnostic IVD medical equipment Test standards Performance limits EN 55022 1998 A1 2000 EN 61000 3 2 2000 A2 2005 EN 61000 3 3 1995 A1 2001 ANSI C63 4 2003 EN 61000 4 2 1995 A1 1998 A2 2001 EN 61000 4 3 2002 A1 2002 EN 61000 4 4 2004 EN 61000 4 5 1995 A1 2001 EN 61000 4 6 1996 A1 2001 EN 61000 4 8 1993 A1 2001 EN 61000 4 11 1994 A1 2001 Class B 150 kHz 1 GHz Class A Class B 450 kHz 1 GHz 4 kV CD 8 kV AD Criteria B 3 V m 80 MHz 2 7 Ghz Criteria A 1 kV Criteria B 2 kV line to ground 1 kV line to line Criteria B 3 Vims 190 kHz 80 MHz Criteria A 3 A m Criteria A 30 500ms Criteria B 60 100 ms Criteria B 100 10 ms Criteria C 100 20 ms Criteria C 100 5000 ms Criteria C Thermo Fisher Scientific warning codes Chapter 15 Troubleshooting Guide properly A Error an
102. ular Requirements for In Vitro Diagnostic IVD Medical Equipment The safety specifications are also met under the following environmental conditions in addition to or in excess of those stated in the operating conditions Altitude Up to 2000 m Temperature 5 C to 40 C Humidity Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Mains supply fluctuations 10 from nominal Installation category ll according to IEC 60664 1 see Note 1 overvoltage category Pollution degree 2 according to IEC 60664 1 see Note 2 Thermo Scientific Multiskan FC User Manual 109 Technical Specifications Safety specifications 110 Thermo Scientific Multiskan FC User Manual Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 2 The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution s
103. urement mode The Fast mode produces fast results with a slightly narrower linear absorbance range than with the Normal mode Number of readings sets the number of measurements per well One means that each well is measured only once and two or more means that the measurement is repeated kinetic The number of readings can be anything between 1 and 100 If the Number of readings is higher than 1 the measurement is considered kinetic and the interval time between the readings must be set Kinetic calculations can be added to the protocol For instructions on how to program a wavelength for example refer to Programming a wavelength on page 65 Thermo Fisher Scientific Thermo Scientific Multiskan FC User Manual 33 Menus and Parameters Main menu Shaking parameters Incubation parameters 34 Thermo Scientific Multiskan FC User Manual You can define the shaking parameters in the Shaking window No Before measurement Background Move OK Select Cancel You can select between no shaking shaking before measurement and background shaking The shaking speed can be set to Slow Medium or Fast Refer to Table 3 1 on page 24 Background shaking is active during the intervals of kinetic measurements The shaker shakes in sections of 5 seconds with the slow speed For instructions on how to program shaking refer to Programming shaking on page 66 You can define the incubation parameters inc
104. ut includes blanks The blank used in the calculations is always an average of the blanks included in the layout A Note The plate layout must include calibrators to enable them for further calculations for example curve fits Refer to Curve fits on page 48 A Thermo Scientific Multiskan FC User Manual 47 Menus and Parameters Processing menu Table 5 9 Processing parameters Calculation Processing Calculation Calculation L Type L No L Linear regression L Conc Transfor L Linear Us Logarithmic L Meas Transfor L Linear b Logarithmic L Extrapolation L Yes L No L Four parameter logistic L Cubic spline L Conc Transfor L Linear ls Logarithmic L Meas Transfor L Linear L Logarithmic L Point to point L Conc Transfor L Linear ie Logarithmic L Meas Transfor L Linear l Logarithmic L Factor L Value L 1 000 Depending on the level of the internal software the FILE key opens a list of actions possible for the Current protocol Curve fits The curve fits types of the calibrators are e Linear regression e Four parameter logistic e Cubic spline e Point to point 48 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Menus and Parameters Processing menu Linear regression Linear regression is a term usually reserved for the simple linear model involving a response y that is a continuous variable and a single explanatory variable x related by the equation y atbx where
105. ware for Multiskan FC User Manual Cat no N07713 The instrument has one computer interface USB When the instrument is connected to SkanIt Software the Remote mode active text is shown on the display The STOP button on the keypad can be used to terminate the computer remote control Thermo Scientific Multiskan FC User Manual 19 Installation Installation of Skanlt Software for Multiskan FC 20 Thermo Scientific Multiskan FC User Manual Thermo Fisher Scientific Front view Lamp and filter wheel chamber cover Measurement chamber door Back view USB port for memory stick Thermo Fisher Scientific Chapter 3 Multiskan FG Main Parts This chapter describes the main parts of the Multiskan FC instrument The front view of the Multiskan FC instrument is shown in Figure 3 9 rzy Display Keypad Figure 3 9 Multiskan FC front view The back view of the Multiskan FC instrument is shown in Figure 3 10 USB port for printer USB port for PC ON OFF switch Cooling outlet D Ry ga 4 7 bed USB USB POWER IN PRINTER PC 24VD e ce E renere REF 51119100 LSN J2s7 z000307 100 240 VAC Ea 50 60Hz 100 VA Manufactured in Finland Thermo Fisher Scientific Oy ara 2008 05 Figure 3 10 Multiskan FC back view Thermo Scientific Multiskan FC User Manual 21 Multiskan FC Main Parts Side view Side view The side view of the Multiskan FC instrument is

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