EHSE Biosafety Safety Manual
Contents
1. Sharps precautions Pathogen Disease s Transmission Standard Risk Infectious Disinfectant Vaccination Vaccinations Routes Controls circle Dose required completed circle Aerosol Gloves Ingestion BSC Inoculation Mask Sharps precautions Aerosol Gloves Ingestion BSC Inoculation Mask Sharps precautions Aerosol Gloves Ingestion BSC Inoculation Mask Sharps precautions Aerosol Gloves Ingestion BSC Inoculation Mask Sharps precautions Aerosol Gloves Ingestion BSC Inoculation Mask Persons conducting the work Person conducting the assessment Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 39 Appendix G Safe Work Procedure Faculty Division Cost Centre Location Function Activity Procedure developed by Approved by Date Personal Protective Equipment Required Please x below to indicate the appropriate PPE required for function activity w Q 0000000606009 60900Q LJ LJ LJ LJ LJ LJ LJ LJ LJ LJ LJ LJ LJ LJ LJ Ll Activity Hazards Identified Risk Score Controls Final Risk Steps in the process task What could cause an injury Score How harmful is it What can be done to minimise the risk of injury Refer to attachment A Apply Hierarchy of Control refer Attachment A Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document2. rcr uu Room numbers cross reference 2 55 Building 42 2 32 33 Building Yellow 2 2 53 53a Building 42 2 34 Building Yellow 2 2 48 Building 42 2 38 39 Building Yellow 2 2 34 34a Building 42 2 68 69 Building Yellow 2 2 54 46 EL Building 42 2 50 Building Yellow 2 2 40 Fume Cupboard Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 30 Charles Darwin University AppendixA PC2 Laboratory Arrangement Building Yellow 3 Laboratory Space Yellow 3 Legend A Fire Extinguishers BioSafety Cabinet Safety Showers Eye Washes First Aid Kits Emergency Egress vd EH p Fume Cupboards EE 1l Hand Wash Sink Cupboard SESE uu I Laminar Flow Unit Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 31 Charles Darwin University AppendixA Quarantine Containment QC2 Laboratory Arrangement Building Yellow 2 Level 2 Parent Facility Number C1012 Room numbers cross reference n n Building 42 2 32 33 Building Yellow 2 2 53 53a C1115 Building 42 2 34 Building Yellow 2 2 48 C1117 Building 42 2 38 39 Building Yellow 2 2 34 34a C1119 Building 42 2 68 69 Building Yellow 2 2 54 46 C1120 n n Building 42 2
3. 6 Implement controls as per risk assessment and complete all relevant training Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 7 Charles Darwin University Hazard Identification Pathogen Risk Groups All microorganisms and pathogenic materials must be assessed against AS NZ 2243 3 2010 to determine which risk group they fall into and subsequently which level PC lab is required for that work Microorganisms falling into Risk Group 2 in AS NZS 2243 3 2010 present moderate individual risk and limited community risk They cause human animal or plant disease but do not pose a serious risk because effective treatment and preventative measures are available and there is limited potential for spread For example Staphylococcus aureus rarely causes life threatening disease in a laboratory situation and is a Risk Group 2 microorganism Human immunodeficiency virus HIV although potentially lethal is also a Risk Group 2 microorganism when not concentrated because in normal laboratory circumstances the risk of transmission is low Classes of Genetic work All work involving genetic work must be assessed against OGTR Guidelines to determine the hazard class of dealing and subsequently the level of PC lab required to conduct the work Classes of dealings involving Genetically Modified Organisms GMOs Register of Micro organisms A register of all micro
4. STAIR 2x44 COMMS ROSCOPE ni STAIR 1 i c hen OFFICE TECHNICIANS m ROOM 1 adi F l i idis PREPARATION 36 oo i 1 26 e 119 ic Y amp Y 7 i Logay 5 rover V E CORRIDOR CORRIDOR E 3 1 608 y neor u 1 E03 A 3 OFFICE ome ornee ores OFFICE ormee OFFICE ES Ree E x ias TECHNICAL a A 1 E08 PREPARATION DOT 1 34 0 AO EN INSTRUMENT ROOM 1 c09 1 43 me 725 TSSTAR 4 LH d NE Ey MUSEUM PREP mue 0 S84 n SHADED i TEACHING LAB 1 TEACHING LAB 2 TEACHING LAB 3 I TEACHING LAB 4 re Ch 1 47 1 46 1 33 1 32 i I MUSEUM 1 1 180 o RR COVERED WALKWAY 1 E07 Charles Darwin me m mivensiry C YELLOW BUILDING 2 LEVEL 1 PLAN LUON 1 3 A8 rA YELLOW BUILDING 2 EEMEL 4 PLAN L2 YE2 01 3 A E CDU 88 Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 33 Charles Darwin University Appendix B Induction Checklist This is designed as an aid to inducting new personnel into the safety practices to be followed in the laboratory facilities All personnel should have access to the Biosafety Safety Manual and be provided with explanations and demonstrations of its content This shall occur before the person commences work in the laboratory Laborato
5. contamination and interruption of airflows cabinets are not designed for protracted storage of materials Wipe the work zone surfaces with fresh disinfectant solution Remove gloves for sterilization or disposal as contaminated waste Allow the cabinet to run for at least another 5 minutes Fit the work opening cover and run the UV lights for another 5 minutes The sump of the Class II cabinet should be cleaned weekly or after any spill Operating Instructions for the BSC in Yellow 2 2 53 Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 12 Charles Darwin University Clyde Apac Class II Cabinet Leave the mains power turned on at all times from the power point Remove the front cover To turn on cabinet a Firmly press run button b Firmly press light button To turn off cabinet a Firmly press light button b Firmly press run button Return front cover Possible ERROR messages Cabinet alarm is activated EXHAUST LOW turn cabinet off and then on again POWER FAILURE press boost button twice Turning on the BSC 1 2 OV e w Remove unnecessary items from the cabinet Run the cabinet with the UV lamp on for 10 15 minutes prior to use with the work opening cover in place Remove the work opening cover and wipe down the work zone surfaces with a suitable disinfectant Run the cabinet for at least 5 minutes so as to clear away any r
6. for when dealing with non infectious museum specimens and plant material microscope slides etc Gloves should be removed on completion of laboratory tasks when using a telephone or when performing any other office duties Hands should be washed with water and liquid soap disinfectant e g Hibicleans and properly dried with paper towel after removing laboratory coat and gloves Hand washing before commencing office work is essential Storage amp Transport of biohazard waste containers Storage All the biohazardous waste material produced by teaching and research facilities covered by this manual is stored upon appropriate containment in the caged area outside Building Yellow 2 Segregation ensures non reactive safe and secure storage Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 25 Charles Darwin University Transport For transport between university campuses or to other locations quarantine infectious waste materials are to be placed in an inner container surrounded by absorbent padding and contained within a durable resistant outer container Please talk to the Technical Services manager or their delegate in Yellow 2 1 26 if you need to do this Unless permission is otherwise obtained the Faculty of Engineering Health Science and the Environment does not have AQIS clearance to use any imported biological products in vivo If
7. in the AS NZS 2243 3 standard CDU has provided this Biosafety Safety Manual to assist researchers and students in continuously improving their ability to use biological hazards in a safe effective manner and also to conform to relevant legislation This manual and the procedures and processes contained within are an element of the risk management process at Charles Darwin University To ensure the proper control of biological hazards at CDU you must always use the following process 1 Identify the biological hazard and governing regulatory requirements When a biological hazard has been identified it must be recorded on your Laboratory Facility Microorganism Biohazard Register and Workplace Hazard and Risk Register After identification of your biological hazard s you must determine what legislation is relevant to the biological hazard you will be using The specific legislation will determine any additional specific controls that may need to be implemented 2 Assessthe risk from the biological hazard using the risk grouping system from AS NZS 2243 3 All biological hazards need to be assigned a risk group 1 4 using AS NZS 2243 3 as the primary assessment tool A risk assessment is to be conducted covering 2 1 2 a j of AS NZS 2243 3 If it is unclear which risk group an organisms or biological hazard belongs to contact your supervisor to assist you It is important to also check whether the biological agent is listed in Table 2 paragra
8. issued with a wrap around laboratory gown Gowns shall e Be worn at all times whilst working in the area e Be removed and hung on the coat rack before leaving the laboratory e Be laundered regularly and when contamination is suspected Visitors to the laboratory will be provided with gowns when there is work being undertaken Laundry arrangements are managed through Technical Services Contact Technical Services for more information Eye Protection Safety glasses shall be worn at all times when work is conducted in the laboratory Over glasses will be provided to those who wear prescription spectacles Certain operations such as handling cryogenic liquids and samples and unloading autoclaves require the use of a protective face shield Gloves Gloves shall be worn e When required by a Safe Work Procedure and Risk Assessment e When handling human blood and body fluids e When handling any microorganism e When working in a BSC Gloves do not provide automatic protection This is due to the fact that even new gloves may have their integrity compromised Consider double gloving Gloves shall be changed regularly washing hands between changes The standard glove available in the laboratory is a latex examination glove Latex gloves have been found to have the best integrity greatest flexibility and sensitivity allowing for dexterity and least permeability Allergic reactions to latex gloves which range from mild skin ir
9. properly The following details need to be considered to ensure that the fume cupboard s performance is not compromised e Do not work within ten centimetres of the leading edge The larger the item the further back it needs to be within the fume cupboard to overcome the turbulence created e Do not place storage items behind the area you are working in This is of particular importance where a Perspex screen or lead bricks are used for radioisotopes e Chemicals are not to be stored in fume cupboards e Minimise the amount of items used within the fume cupboard when working e The amount of flammable solvent placed in a fume cupboard should be minimised and the subject of a risk assessment e Do not put large equipment such as ovens in the fume cupboard as they block the baffles and produce regions of zero or low flow in the workspace e Always have the sash as low as possible during the work e Minimise traffic past the front of the fume cupboard as this can cause turbulence and result in fume escape e The laboratory doors near the fume cupboard should be kept closed during its use e The make up air supply and room ventilation should be on whenever the fume cupboard is in use Fume cupboard performance should be checked every 6 months to ensure adequate face velocity an average of gt 0 5 ms and laminar flow This testing is arranged by CDU Technical Services through the Technical Services Manager The cupboard should be checked to ensur
10. purpose of cleaning or facility maintenance including maintenance of BSCs to ensure they are aware of existing hazards All personnel should be provided with explanations and demonstrations of laboratory hazards This shall occur before the person commences work in the laboratory Personnel are asked to verify they have received this information and may be asked to confirm that they have understood it by way of questioning and demonstration This checklist represents a record of induction and should be kept in the Induction folder Room No s Supervisor Department Item Check Demonstrate facilities Laboratory Access requirements Location of fire fighting equipment Location of first aid kit Evacuation procedures Emergency eye wash and safety shower Location of safety documents Personal Protective Equipment requirements Hand washing procedures explained and demonstrated Waste disposal procedures Spill procedures Decontamination requirements Other items User s Name Signature Date Inducted by Supervisor Acknowledgment Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 35 Charles Darwin University Appendix D Safety Training Plan and Assessment Training element competency Date assessed Check WORK PRACTICES AND PROCEDURES Explain the
11. rules about Laboratory access Select explain and demonstrate the correct use of personal protective equipment Explain the use of other safety equipment amp devices pipettes loops etc List factors that may affect susceptibility resistance or consequences of infection Outline the evacuation procedures including the location of fire fighting equipment first aid kits evacuation plans Demonstrate knowledge of the location of all safety documentation Explain and demonstrate hand washing procedures Outline waste disposal procedures Outline spill management procedures Explain the incident reporting procedure Explain the hazard of exposure of service personnel to biological materials Demonstrate knowledge of appropriate selection of decontamination protocols chemicals steam UV radiation etc REGULATORY LEGISLATION STANDARDS and GUIDELINES Demonstrate knowledge of relevant legislation including Acts Regulations Demonstrate knowledge of relevant CDU policy procedure guidelines Interpret and apply the relevant OGTR Guidelines Demonstrate knowledge of the standards guidelines that apply to the classification of biohazardous agents according to risk Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 36 Charles Darwin University Training element competency D
12. v x x Applications Bench tops v v v p p x Liquid disposal v v p p x x Glassware v v v p v v Instruments v v OE v OE x lt x lt x lt Equipment total x x x v x x decontamination With care some plastic and metal components may be affected by contact with these substances Consult User Manual or technical staff for clarification prior to using Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 23 Charles Darwin University From Appendix B to Procedure 2 6 2 Biological Waste Table 1 Disinfectants Antiseptics used in PC2 laboratories Disinfectant Active Constituent Recommended Concentration Chlorhexedine Gluconate 296 equivalent to Chlorhexedine 11 3g L 2 096 w w and Tartrazine MICROSHIELD CHLORHEXIDINE 2 Potassium peroxymonosulfate Sodium 196 solution Virkon polyphosphate f 1 tablet dissolved in 500 ml water Sodium dodecylbenzenesulfonate Bleach commercial Sodium hypochlorite 4 m v minimum Gel Hand Sanitizer Ethanol gt 70 Important things to wipe daily include For students bench tops sink taps pipettes For technicians e Door handles e Preparation area e BSC e Lab equipment including incubator doors and handles Waste Management Chemical Biological and General Waste The waste management procedures used by the School is defined in Technical Servi
13. window should never be moved from the normal operating height unless during loading or unloading of materials apparatus into cabinet e The alarm will be activated whenever the sash window is moved from the normal operating height e Whenever the sash is moved to the correct height from higher or lower positions the light will automatically come on as a signal to the user e The sash window may be opened to maximum position for the purpose of loading unloading of material apparatus into the cabinet When the sash window is fully opened the alarm will sound this may be muted by pressing the MUTE button but it will automatically sound again after 5 minutes to remind the user that it is not safe to work in the cabinet and the light will be turned on to facilitate cleaning Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 11 Charles Darwin University Turning on the BSC 1 Raise the sash to the indicated normal operational height readystate The lamp will turn on when the height is reached 2 Turn on the fan by pressing the FAN button Input Fan PIN if asked This will start the warm up procedure default 3 minutes All buttons are disabled during warm up period 3 The BSC is ready for work Turning off the BSC 4 Turn off the fan by pressing the FAN button Input the Fan pin if asked This will start the post purge procedure default 0 minutes All but
14. you want to import any material from overseas please contact the Technical Services Manager Yellow 2 1 26 ext 6349 and they will help you with the AQIS web site to gain a permit Definitions In vitro in glass referring to a process or reaction carried out in a culture dish or test tube In vitro use includes those procedures which do not involve direct or indirect exposure of the material or its derivatives to humans or animals Examples of in vitro use are The use of media for bacterial culture as long as the bacteria are not exposed to humans or animals The use of cell lines as long as either the cell lines or material associated with that cell line is not exposed to humans or animals In vivo in the living organism is where biological product or its derivatives are directly or indirectly exposed to a living organism Consequently use in micro organisms can be regarded as in vitro unless the treated microorganisms come in contact with animals n vivo use includes those procedures which do involve direct or indirect exposure of the material or its derivatives to humans or animals Examples of in vivo use are vaccine production where the vaccine will be used in animals or humans usein laboratory animals or clinical trials in humans the use of media to produce vaccines or other substances for use in animals or humans the use of sera or albumin for embryo washing where th
15. 27 Building Yellow 2 2 49 POST tr o E rg er PUNT GRADUATES lt l rein RI ps m m a RESOURCE orrice ornce ornce orrice oFFice orrice orrice orrice orrice oFFice oFFice orrice OFFICE ROOM ex orice 2 61 260 259 258 257 tf 247 235 233 232 230 228 227 226 eat ay LOB Y em J A LEL m t Lr E pod CORRIDOR 2 003 vL ou f ET lt J 2 c02 Lr3 3 q 4 d i t iR ron OFFICE JOFFICE OFFIcE RECEPTION 3 E 2 01 2 03 re ROOM OFFICE 12 50 2 C04 OFFICE OFFICE OFFICE 2 07 2 08 2 09 m UE mae e z ven ait C Yettow BuILDING 2LEvEL2PLAN PP YE2 02 3 A atas AT A3 Finance DmCDU CAD BH F YELLOW BUILDING 2 LEVEL2 PLAN Charl and Asset Servicer Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 32 Charles Darwin University AppendixA Quarantine Containment QC2 Laboratory Arrangement Building Yellow 2 Level 1 MEZZANINE PLAN Parent Facility Number C1012 GENERATOR Room numbers cross reference Building 42 1 21 22 Building Yellow 2 1 28 GENERATOR FUEL TANK LEO FIELD STORE 1 GENERATOR MEZZANINE Site i 1 net Post coLocv ecoLocv ecoLocv ecoLocY AASE Y GRADUATES LAB 1 LAB 2 LAB 3 LB 4 aaa ca t E A 1 01 1 03 1 05 1 07 1 09
16. 946 6848 Lyn Lowe Technical Officer Yellow 3 8946 7537 Ellie Hayward Truc Nguyen Teaching rep 8946 6881 Anna Padovan Research rep 8946 6555 Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 5 Charles Darwin University Access Routine access is only provided to persons that have 1 Completed the General Laboratory Safety Induction 2 Completed the PC2 Laboratory Induction Appendix B 3 Issued with the all required personal protective equipment 4 Have been authorised as a laboratory user by the area leader or their delegate 5 Satisfied all the requirements of the Faculty IBS including all paperwork and approvals AND OR the Darwin Region IBC 6 Satisfied all the requirements of DAFF including all paperwork and approvals The laboratory shall be kept locked at all times when not in use Only authorised persons shall be provided with a key or access card for the laboratory Where access is required for the purposes of maintenance or cleaning this will only be provided to persons that have 1 Completed the Cleaner Maintenance Induction Appendix C 2 Demonstrated that they have any required personal protective equipment 3 Beenauthorised by Faculty IBS 4 Beenauthorised as a laboratory user by the Technical services manager or their delegate All records of inductions and authorised persons will be maintained by the facility lab ma
17. a Jg c ta oe WORLD Scenery BIOSAFETY SAFETY MANUAL Includes PC2 Physical Containment QC2 Quarantine Containment General Laboratory Version No 2 0 Version Date 16 January 2015 Approved by Faculty of EHSE Workplace Health and Safety Committee Chair signature Next Review Date 16 January 2016 Developed using existing Charles Darwin University and Newcastle University documents and forms Charles Darwin University Contents Dipl me 4 Nature OF WORK C 5 i e rci 5 Approval S soiien 5 Wo crime 5 ha I Me 6 Nc a ES 6 Risk Managemient esistere etes eM et E ei Mer ue 6 Hazard Identification enero ntn ta rata rad uia rna nd tx uuu Fa aka tuna Ea Yao Ra uaa 8 Pathogen RISK Groups e dt ot e c i et e e eee ades cete duae decades E Rn Ou ecu Ds a i eoe ea 8 Classes of Genetic WOEK rr rr E TERR RE RESI PU ues tee ds Peru ke Ree meine 8 Register of Micro orgaNi SMS ceto ee seien eee desee eet cete esten e deese exe ceto esa eee rud erede eut oet eges E eA 8 Risk ASSCSSING i e T Y 8 Assignment Of RiSk GroUp ccccessessscecceecessesenaeceeececesse
18. and Release your grasp of the first glove you removed Pull your second hand free from its glove Dispose of the gloves into a bio hazard bag Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 21 Charles Darwin University Working Alone this is for research staff and students only In general working alone in a PC2 laboratory does not in itself increase the risk of being injured In addition Risk Group 2 microorganisms do not pose an immediate threat to the health of anyone exposed to them For these reasons it is only work with Risk Group 3 or higher organisms that is considered of sufficient risk to preclude working alone The primary concerns with people working alone are e The possibility that they undertake activities without appropriate training or approvals or without using standard practices e The increase in risk associated with persons actually being alone particularly with regard to personal safety e The lack of access to the emergency response support particularly first aid in the event that the person is injured or requires medical attention The following issues therefore need to be considered when contemplating work after hours eThe nature of the work eThe capacity of the person to conduct it that is their experience and training e Additional risk factors such as any medical conditions eThe means of communic
19. ard interface suitable for novice to intermediate users ChemGold III is the advanced interface suitable for advanced users Health Safety and Environment run training sessions for CDU staff For further information email Health Safety and Environment or phone 8946 6473 Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 9 Charles Darwin University Risk Control Controlling risk must be done by applying the hierarchy of controls 1 Elimination does the work with the microorganism need to be done 2 Substitution can a microorganism in a lower Risk Group or of lower overall risk be used to do the work 3 Isolation can the microorganism be completely contained during the work 4 Engineering controls eg use a biological safety cabinet 5 Administrative controls safe work procedures and practices eg laboratory rules like no mouth pipetting Modify the work system or process eg use lower concentrations or smaller quantities 6 Personal Protective Equipment is used eg laboratory gowns gloves Physical Containment of microorganisms represents a combination of engineering and administrative controls coupled with the use of personal protective equipment However elimination and substitution should always be considered prior to starting work with a given pathogen Primary barriers to transmission of infectious agents are provided by e
20. ate assessed Check Demonstrate knowledge of the requirements applicable to Quarantine Containment Demonstrate knowledge of Primary and Secondary barriers APPROVALS Describe the role of and function of the Institutional Biosafety Committee Outline the procedure for getting approval for working in PC2 facilities Outline the procedure for getting approval for working in QC2 facilities LAB SKILLS AND EQUIPMENT Demonstrate the correct use of Class II Biosafety cabinets Describe the limitations other extractive equipment such as fume cupboards Demonstrate the correct use of autoclave User s Name Signature Date Completed by Supervisor Signature Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 37 Charles Darwin University Appendix E Register of Microorganisms Laboratory Project Chief Investigator Microorganism Risk Location Box Culture Source Strain Type MSDS Import Comment Group No No Y N Permit No Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 38 Appendix F Microorganism Risk Management Form Microorganism Summary Hazard Identification amp Risk Assessment This should be completed for each disease causing microorganism with reference to available safety data
21. ation available Note CDU Security should be notified so that they are aware of persons present in the building Where after hours work is necessary this should be authorised by Head of School supervisor or Technical Services Manager or their delegate When doing after hours work you must sign in sign out the afterhours book for your area Work Procedures Inoculating Loops These should be sterile before and after use To avoid spatter and aerosol generation whilst sterilising loops in a Bunsen flame slowly draw the wire through the tip of the blue cone starting at the base of the wire and ending with the loop The loop should be completely closed Note there is the potential to contaminate loop handles when taking samples from deep tubes If this is likely to occur handles should be decontaminated by standing in disinfectant solution This can be avoided by using sample tubes and loop wires of appropriate lengths where possible Sharps Sharps are essentially anything that has the potential to penetrate the skin but are typically needles scalpels Pasteur pipettes and broken glassware The main risk with using sharps is self inoculation e Keep the use of sharps to a minimum e Do not bend needles or try to recap them after use Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 22 Charles Darwin University e Use blunt cannulas wh
22. aving the laboratory after removing laboratory gown e Between glove changes e Whenever you suspect contamination Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 18 Charles Darwin University Hand Washing Technique 1 Move from the palms to the inside surfaces of the thumb changing from left to right hand 2 Intertwine fingers of both hands and work them back and forth to full length of fingers on each side 3 Move over to the backs of the hands and then to the wrists giving it a few twists around the wrist 4 From the wrist move the hand on top over the backs of the fingers including the thumb on the hand below 5 Intertwine the fingers of both hands again to cover the webs of the fingers 6 Rub the nails and fingertips back and forth over the palm of the opposite hand Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Charles Darwin University Personal Protective Equipment Clothing amp Footwear Requirements It is a University requirement that enclosed footwear must be worn at all times in the laboratory no bare feet thongs or sandals at any time The main door of the laboratory indicates this requirement which applies to any person entering Laboratory Gowns Each person carrying out work in a PC2 designated area will be
23. ces Procedure 2 6 Waste Management and related procedures 2 6 1 Chemical Waste 2 6 2 Biological Waste and 2 6 3 General Waste These procedures specify the treatment regime for each particular type of waste before disposal Specific information on handling storing and labelling requirements as well as accidental containment measures are also given Refer to the Flowchart at the end of Procedure 2 6 for an overview of the waste management process Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 24 Charles Darwin University The bulk of the quarantine and potentially infectious biological laboratory waste of the School is disposed of after autoclaving on site via authorised waste management contractors To protect the community and the environment the following standard safety measures apply 1 Waste must be segregated contained and properly packaged at the point of production This approach reduces double handling and minimises the risks associated with contamination and spillage breakage 2 Bags for disposal should be sealed by laboratory staff and not left open at the end of the day nor left lying in laboratories where they can be inadvertently removed by cleaning staff 3 Quarantine or potentially infectious material should not leave the laboratory until chemically treated and or autoclaved 4 All medical waste must be kept separate from the quara
24. d understood the contents of this manual before you commence any work within the PC2 facilities The manual is arranged so that sections align with legislation standards and industry wide practices Each section contains relevant information ideas and suggestions of risk management in that topic and some references to other information or resources which you should peruse or use This manual should be seen as additional to any other Laboratory Safety information available or provided at Charles Darwin University The information contained within this manual is comprehensive but not exhaustive and you may need to do further research reading of other material to ensure all contingencies are covered This manual is applicable to any spaces for designated as approved for PC2 and Quarantine work in Building Yellow 2 and Yellow 3 Casuarina Campus This manual refers to a number of additional sources of information including e Safety Data Sheets This provides safety information about chemical substances and some biological agents in use Available from supplier or ChemWatch e Equipment folder which contains operating manuals procedures and test and maintenance records e Induction folder which is a record of induction and training undertaken by personnel using the laboratory e Risk Management folder which contains risk assessments safe work procedures of procedures undertaken in the laboratory These folders are located in the foll
25. e clean up between procedures Plan work so as to place all materials in or close to the cabinet and within easy reach of the operator Allow the cabinet to run for a further 5 minutes before use Turning off the BSC At the end of work leave the cabinet running and conduct the following procedures Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 13 Charles Darwin University Transfer cultures to a container for storage or incubation Disinfect and remove all unnecessary materials to reduce the potential for cross contamination and interruption of airflows cabinets are not designed for protracted storage of materials Wipe the work zone surfaces with fresh disinfectant solution Remove gloves for sterilization or disposal as contaminated waste Allow the cabinet to run for at least another 5 minutes Fit the black cloth and run the UV lights for another 5 minutes The sump of the Class II cabinet should be cleaned weekly or after any spill B SO urge w UV lamps in Biological Safety Cabinets The BSC is fitted with germicidal ultraviolet UV lamps in the work zone UV can be a useful adjunct to surface cleaning procedures but should not be seen as a replacement for good cleaning technique e Laboratory gowns and safety glasses face shield suitable for UV work must be worn at all times when UV is operating All work opening covers should be in
26. e embryo is destined for implantation either in humans or animals the use of biological material for the culture of organisms or in a cell line which will be exposed to animals plants or humans theuse of bovine pituitary extract in the production of skin grafts for experimental or clinical use in animals or humans Non infectious waste Non infectious material such as waste paper and plastic products is collected in the waste bins lined with black plastic bags Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 26 Charles Darwin University Infectious waste generated in the laboratory consists of several types each of which has different disposal routes Sharps All sharps areto be disposed of by placing them in the sharps containers which will be on the benches in the facility When these reach 7596 full they should be replaced For replacement and removal of sharps containers contact Laboratory Manager or Technical Services Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 27 Charles Darwin University Vaccinations this is for research staff and students The table below provides a list of Risk Group 2 pathogens where prophylactic vaccination is available and may be indicated Table 2 Vaccinations that are available for infectious a
27. e is has a current test sticker prior to it being used Records of testing are maintained in the Equipment folder in the facility Pipettes Mechanical or electronic pipettes are to be used for all pipetting tasks Never pipette anything by mouth Because pipette tips can pierce a biohazard bag they should be treated as sharps when possible do not remove them from the pipettes by hand and dispose of them in a sharps container at the bench The action of pipetting can form aerosols 1 Pipette slowly particularly when using pipettes for mixing to avoid aspirating aerosol or liquid into the pipette body 2 Where aerosol transmission is a risk carry out pipetting operations in a BSC 3 Filtered tips or filter plugs may be required to avoid sample cross contamination Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 15 Charles Darwin University Use and care of pipettes General use instructions Never overwind the mechanism when setting the volume Avoid bringing the body of the pipette into contact with the vessel you re pipetting from Spray or wipe the body of the pipette over with disinfectant after use and store it upright If infectious liquids are aspirated into the pipettes do not continue to use the unit Disassemble the unit in a BSC wearing gloves and decontaminate the components by soaking in disinfectant solution NOTE Unde
28. e microorganism but may also be a function of the type of operations undertaken with it For example diagnostic blood samples can be handled under Level 2 containment conditions but concentrated cultures of HIV must be handled under Level 3 conditions As a result selection of risk control measures should be based on an assessment of the tasks to be undertaken and information about the pathogen s involved NOTE All human specimens must be risk assessed for work at PC2 level Safety Information Safety data sheets SDS or other safety information related to the microorganisms and chemicals are available in the SDS folder In addition there are a number of on line sources of safety information including e Public Health Agency of Canada Laboratory Biosafety and Biosecurity http www phac aspc gc ca lab bio index eng ph e Public Health Agency of Canada Pathogen Safety Data Sheets and Risk Assessment http www phac aspc gc ca lab bio res psds ftss index eng ph e Centre for Disease Control http www cdc gov od ohs biosfty bmbl4 bmbl4toc htm e Office of Gene Technology Regulator http www ogtr gov au e Quarantine DAFF http www daff gov au quarantine e AS NZS 2243 3 2010 Safety in the laboratory Part 3 Microbiology e ChemWatch the chemical management system in use at Charles Darwin University It allows access to Safety Data Sheets SDS database and manages chemical inventories manifests ChemFFX is the stand
29. eh coun toes usununce aun ode icr iei US nennen enn 23 Waste Manageme eM 24 Chemical Biological and General WaSte cccccccccsssessssececccecessesnsseeececsssesesaeaeeeeecesseseaaeseeeeeeesseseaaeaeeeeseesees 24 Quarantine and Deemed Quarantine Waste ccsccccecssececsesneeecseseeecsesaeeeeseaeeeceesaeeeeseaaeeeeseaaeeeeeeaaeeeeseaas 25 Personal Safety and Waste Manageme n cscssccccccecsssessssececeescessesesecececeseesesaeaeeeeseessesauaeeeeeesseeseseeaeeesess 25 Storage amp Transport of biohazard waste containers esses eene nnn nnne neni nn nnns nnn 25 Definitions acre T 26 Vaccinations this is for research staff and students 28 1 E E E 28 AppendixA PC2 Laboratory Arrangement Building Yellow 2 sssssssssssssssssssrresssssseserresssssserernessssssreernesse 30 AppendixA PC2 Laboratory Arrangement Building Yellow 3 eese 31 AppendixA Quarantine Containment QC2 Laboratory Arrangement Building Yellow 2 Level 2 32 AppendixA Quarantine Containment QC2 Laboratory Arrangement Building Yellow 2 Level 1 33 AppendixB Induction Checklist cedro bo e EREE aee a aee E EDU ets 34 AppendixC Cleaner Maintenance Personnel Induction Checklist esee 35 AppendixD Safety Training Plan and Assessment sse nennen nnns nenne neni n
30. ere possible e Discard sharps into sharp containers Decontamination For the safety of all laboratory users items used in conjunction with infectious material must be decontaminated when they are finished with This is achieved by e wiping with a disinfectant e Soaking in a disinfectant sterilant bactericide viricide or e Autoclaving pressure steam sterilising The choice of decontamination procedure will depend on the microorganisms involved the presence of other materials chemicals radioisotopes organic material etc and the equipment to be cleaned Chemical disinfectants are used for routine decontamination and spills It is important that a decontaminant is effective against the microorganisms being handled is selected and available before work commences Table 1 is provided to assist with the selection of disinfectants Table 1 Disinfectant selection a bold tick indicates that the disinfectant is preferred for the application agent Virkon Hypochlorite Alcohols Formaldehyde QUATS lodophors bleach Gas Concentration 296 1 596 70 8596 5g m 0 1 296 0 596 Contact time mins 10 20 10 20 10 30 600 900 10 30 10 30 Effective against Vegetative bacteria v v v v v v Bacterial spores v p p v P x Lipophillic viruses v v v v v v Hydrophilic viruses v v x lt v x x Fungi v v x v p v HIV v v x v v v HBV v v p
31. esidual aerosols If necessary use plastic backed absorbent sheeting to reduce clean up between procedures Plan work so as to place all materials in or close to the cabinet and within easy reach of the operator Allow the cabinet to run for a further 5 minutes before use Turning off the BSC P NOM Pw At the end of work leave the cabinet running and conduct the following procedures Transfer cultures to a container for storage or incubation Disinfect and remove all unnecessary materials to reduce the potential for cross contamination and interruption of airflows cabinets are not designed for protracted storage of materials Wipe the work zone surfaces with fresh disinfectant solution Remove gloves for sterilization or disposal as contaminated waste Allow the cabinet to run for at least another 5 minutes Fit the work opening cover and run the UV lights for another 5 minutes The sump of the Class II cabinet should be cleaned weekly or after any spill Westinghouse Class II Cabinet Turning on the BSC 1 2 3 pi Remove unnecessary items from the cabinet Run the cabinet with the UV lamp on for 10 15 minutes prior to use with the black cloth in place Remove the black cloth after switching off the UV lamp and wipe down the work zone surfaces with a suitable disinfectant Run the cabinet for at least 5 minutes so as to clear away any residual aerosols If necessary use plastic backed absorbent sheeting to reduc
32. ess it is decontaminated Do not bring in anything other than your practical manual into this space especially not personal equipment like mobile phones calculators or pens You will be provided with a Laboratory gown and have access to appropriate gloves These items MUST be left in the laboratory at the conclusion of working session Eating drinking smoking applying cosmetics and handling contact lenses is forbidden at all times in the laboratory Storage of food items in the lab is prohibited unless they are used as part of experimental activities Long hair is to be tied back at all times Pipetting by mouth is strictly prohibited Always use the pipettes supplied Laboratory doors must be kept closed when work is in progress Doors must be locked when facility is not in use All benches and equipment must be decontaminated at the end of each working session Use the Virkon provided and or decontamination solution specified by lecturer technician All cultures solutions and materials must be clearly identified including dates Only self adhesive labels are to be used to prevent moistening labels with the tongue Smelling sniffing of any cultures is prohibited Gloves are to be removed as per the glove removal procedure outlined in this manual and placed in the Biohazard bin Wash your hands as per the hand washing procedure outlined in this manual before you leave the facility Your safety and that of others depends on the level of y
33. euaeeeeececessesaeaeeeeecsseceesaeaesesecsseesaaeaeeeeseuseeseuaeaeeesesuseesenaeas 8 Safety Informatiot uote excede ecce ec tette cdeavasadessauouadeevanadecedd asdesvatalecesuensteseueatccssdeeclcavaveseess ike 9 RiSk CODnIFolunssonsi ud acr GUa6 ROrcR D EDU IU MG SLE IINE KI du AUR S rU dE CRD S D EUR RDUM DNE CC EON DN Mud 10 Engineering COonlrols iisaassasiukc ka CH vh 4o aXu EUR ERERUEEE ADAM SAN ER IE UR UE EUER NI REN RAS EE UE FRI FRI RES SALE UE DA ERR M MEE 10 Biological Safety Cabinet BSC 00 ectetuer eset e EIS e Seni Rd vete iN noe ed 10 UFIToHrklep D I 11 Operating Instructions for the BSC in Yellow 3 1 05 esses nennen nnne nennen nnn 11 Operating Instructions for the BSC in Yellow 2 2 34a cccccccccccccsssssssecececesscsssneseceeecessesssaeaeeeeeesssesesaeaeeeeseesees 12 Operating Instructions for the BSC in Yellow 2 2 53 esses ennemis nnn 12 UV lamps in Biological Safety Cabinets cccccssscccececessesssaesecececesseeaaecececesseeeaeaeeeeecesseseaaeaeeeeseesseseaaeeesess 14 Testing amp Maintenance of BSC sessi eene nennen enne thi nass asses eise tasa dass isis sesta a asas assess sa aan ns 14 FUME GUPDOGIS eR 14 PIDettes oec eeu E ENEE ees PORE nec aues ien cect Geene dalled eacideSoucudete uad usu ide EE ee 15 Administrative COMI OS iios kxend p on UC dU ronde nas GUI n ObdbilboR KS SEDI M DNE 17 General Laboratory Rules torrente
34. gents those shown in bold type are recommended for health care laboratory workers Bacteria Viruses Clostridium tetani Hepatitis B Corynebacterium diphtheriae Influenza recent isolates Measles Mumps Rubella Neisseria meningitidis Varicella Mycobacterium tuberculosis i Coxiella burnetii Vaccinia Salmonella typhi Hepatitis A Polio Ihese should only be considered Risk Group 2 when present in clinical or food samples Work with wild polio virus is subject to additional restrictions imposed by the World Health Organisation Where any of these pathogens is be used in the laboratory all personnel using the facility should be considered as candidates for vaccination Advice and vaccinations can be provided through the Faculty IBS medical practitioner and the costs borne by the project funds The vaccination provider will maintain records of vaccination Many users will have received vaccinations for a number of the agents listed above A vaccination record for each user Appendix H will be maintained by the user and Faculty IBS There may also be a recommendation for health surveillance such as chest X rays serum sampling etc Work with pathogens may pose elevated risk to certain classes of people Whilst the risks of exposure is the same for all persons who adopt the specified control measures people who are more vulnerable to infection such as those who are pregnant or diabetic or otherwise immu
35. ining volatile toxic or radioactive chemicals Use of BSC Points that should be noted about BSC operation include the following e Check that the BSC is in test before use certification sticker should be on the BSC e All materials should be externally decontaminated before they are introduced to a BSC eKeep clean and dirty materials separated inside the cabinet e Minimise rapid air movement near the cabinet opening such as that caused by people walking past to maintain the laminar air flow e Do not use Bunsen burners in Class Il cabinets use disposable loops or electric heating instead e Don t use centrifuges inside a BSC eKeep the front intake grilles clear eKeep the exhaust discharge clear at least 60 cm in order to allow free air flow and access for maintenance Do not store items on top of top exhaust cabinets e Before starting work at the BSC adjust the laboratory stool to ensure that your e Forearms remain parallel to the work surface most of the time e Lower back is supported e Head is upright e Feet can reach the footrests Operating Instructions for the BSC in Yellow 3 1 05 ESCO Class II Cabinet Using Sash Window e The sash window should be fully closed when the cabinet is not in use This helps keep the work zone interior clean e The sash window should always be in the normal operating height at all times when cabinet is in use Even if the cabinet is left unattended and the blower is on the sash
36. is uncontrolled when printed Page 40 Risk Matrix STEP 1 Consider the CONSEQUENCES STEP2 Consider the LIKELIHOOD STEP3 Calculate the RISK Consider what could reasonably have What is the likelihood of the consequence 1 Select the appropriate column for Step 1 on the matrix happened as well as what actually happened identified in Step 1 happening Consider this below without new or interim controls in place 2 Select the appropriate line for Step 2 rating Look at the descriptions and choose the one Look at the descriptions and choose the one 3 Circle the risk score where the two intersect most suitable most suitable Consequence Description Likelihood Description Major Moderate Minor Insignif Death or extensive injury Major A Is expected to occur Moderate Medical Treatment B Could probably occur Minor First Aid Treatment C Could occur but only rarely Insignificant No Treatment D May occur but probably never will Hierarchy of Control Determine appropriate controls to minimize the risk of injury with priority being the elimination of the hazard s contributing to the occurrence Elimination remove the hazard Substitution use an alternative Isolate separation from hazard Poe oN dp Redesign change equipment or process Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 41 5 Admi
37. nager and retained in the Inductions folders in the relevant building Working safely is a condition of access to the PC2 facility Repeated failure to observe safe working practices and procedures will result in the withdrawal of access privileges Training During their time in the laboratory users will be required to develop and ultimately demonstrate competence in the range of practices required to work safely in the laboratory Appendix D provides a list of these competencies Timing of the training program and assessment will depend on the nature of the work undertaken the current level of experience of the person and their need to work unsupervised These records should also be kept in the Induction folder Risk Management Risk Management is the process of recognising situations that have the potential to cause harm to people or property and doing something to prevent this from occurring or minimising the harm that may be caused The risk management process consists of well defined steps that lead to informed decisions about controlling the impact of risks These steps are Hazard identification Risk Assessment Risk Control Evaluation amp Review a A Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 6 Charles Darwin University This manual covers each of these steps with regard to risks specific to Risk Group 2 microorganisms
38. nclosures such as biosafety cabinets and personal protective equipment The laboratory facilities or work area provide a secondary barrier which acts to contain the agent in the event of a failure of the primary barriers The combination of primary and secondary barriers and work practices constitute the level of containment that must be used to keep the risk of exposure of laboratory workers and the outside environment to acceptable levels An important point that should not be overlooked is that the work practices are integral to the maintenance of these barriers Laboratory equipment and design contribute to safety but only if they are used properly by people who are trained and where necessary supervised It is critical that all laboratory users are trained in the correct use of personal protective equipment in the monitoring of the facility and its equipment and in the practices that should be followed while working in the laboratory All users and lab workers must be advised of all the controls needing to be implemented to control the risks Document the controls as part of a carefully considered risk assessment on the approved form Engineering Controls Biological Safety Cabinet BSC Where there is the potential of generating aerosols containing infectious microorganisms the primary barrier of choice is the Class Il Biological Safety Cabinet Class II BSC There are several standards that apply to the design and use of these cabinet
39. nes Approved by Faculty IBS 4 EXEMPT AND OR NLRD Notifiable Low Risk Dealings OGTR approval required The Laboratories Appendix A provides an illustration of the laboratory configuration equipment arrangements and location of emergency equipment The following description should be read in conjunction with Appendix A Approvals All work in PC2 QC2 spaces must be approved through the formal arrangements and on the approved forms Faculty of EHSE Workplace Health and Safety Committee is the approver of the work in the PC2 QC 2 facilities This will take the form of assessment approval by a Biosafety committee as a sub committee of the Faculty IBS The Biosafety committee will comprise a group of knowledgeable persons who can competently assess the Biosafety risks and hazards and will usually have the following membership Manager of Technical Services research academic teaching academic and any expert invited guest to provide the necessary information required to make a proper informed decision e g mechanical engineer specific scientific expert Contacts Further information about the operation of the PC2 QC2 spaces can be obtained from the following people Name Role Contact No Natasha Lawrence Chair Faculty Workplace Health and Safety 8946 7134 Committee Himi Ibraham Manager Technical Services 0477391292 Martin Boland Chair Faculty IBC 8946 6360 Ellie Hayward Senior Technical Officer 8
40. nistration change work practice 6 Personal Protective Equipment i e gloves glasses hearing protection Safe Work Procedure Action Plan Recommended New Controls Specific Action Accountability Target Date Completion Date Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 42 Appendix H Record of Vaccination Form Name Signature Date Past Illness Year of last Date of last Comments circle childhood vaccination vaccination other Diphtheria Tetanus Polio Measles Yes No Pertussis Yes No Mumps Yes No Rubella Yes No Hepatitis A Yes No Hepatitis B Yes No Tuberculosis Date of test Mantoux Test BCG Vaccine only if Mantoux Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 43 This page intentionally left blank Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 44
41. nns sensn nian ns 36 Appendix E Register of Microorganisms ceseesesesee eene enne nnne nennt nina nass sanis tasa sas s assassin gans 38 AppendixF Microorganism Risk Management Form ccsecsccccccecessesscececeeecesseseaececeesseeseasaeseeeeesseseaaaes 39 Appendix G Safe Work Procedure cccsssscccceceesssesnececececesseseaaeaeeeeecessesaaaeseeecscessesaaaeseeeesseesesaeaeeeeeesseeseaaaas 40 Risk MANI OECD TED LIU DID OL T O E ILS LL ODIS LLLI TOR OORO OTEO 41 Safe Work Procedure Action Plan oer tertie tene eene et eoa ea eran nn eae na NESNESE ee eua a eras Ov teas 42 Appendix H Record of Vaccination Form ccsessscccccecsssssscsececececsssesseaeseeeescssseeuaeeeeecssesseaeaeeeeeessnesessaaeeeeess 43 Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 3 Charles Darwin University Introduction This manual contains information and procedures applicable to field work involving all staff students and volunteers of Faculty of Engineering Health Science and the Environment EHSE at Charles Darwin University engaged in work within in the PC2 facilities This manual is to document safe operating practices required in order to achieve level 2 containment and is the basis for training all laboratory personnel who wish to use the facility Safety in the laboratory is an important part of your work at CDU and you must have read an
42. no compromised should notify and seek advice from the Biosafety committee if necessary Women of child bearing age need to be aware of the risks to an unborn child or themselves of exposure to certain microorganisms such as Toxoplasma gondii Listeria monocytogenes Coxiella burnetii Rubella virus References 1 Australian and New Zealand Standards e AS NZS 2243 3 1995 Safety in laboratories Part 3 Microbiology e AS 2647 2000 Biological Safety Cabinets Installation and use e AS 2252 1 1994 Biological Safety Cabinets Part 1 Biological Safety Cabinets Class I for personnel and environment protection e AS 2252 2 1994 Biological Safety Cabinets Part 2 Laminar flow biological safety cabinets Class I for personnel environment and product protection e AS 2243 8 2001 Safety in laboratories Part 8 Fume Cupboards Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 28 Charles Darwin University e AS 4834 2007 Packaging for surface transport of biological material that may cause disease in humans animals and plants 2 Gene Technology Act 2000 3 Office of Gene Technology Regulator OGTR http www ogtr gov au internet ogtr publishing nsf Content home 1 4 Quarantine Act 1908 and Quarantine Regulations 2000 http www daff gov au agis quarantine legislation quarantine 5 Department of Agriculture Fisheries and Forestry DAFF ht
43. ntine waste until an appropriate waste bin is ordered 5 Material once autoclaved should be placed in the appropriate bin for removal by a specialised contractor 6 Only non infectious waste can be disposed of in domestic waste No waste material is to be removed from the facility without the clearance of Technical Services Manager or delegate Quarantine and Deemed Quarantine Waste For detailed waste management procedures related to Quarantine and Deemed Quarantine Waste refer to 3 4 AQIS Dealing with Quarantine Material and 5 7 Autoclaves amp Sterilisation Guidelines Personal Safety and Waste Management Standard precautions should apply when handling all laboratory waste products All types of biological waste are deemed to be potentially hazardous either infectious pathogenic or toxic mutagenic The person generating the waste is responsible for the correct segregation and disposal of the waste material Clean up your mess before leaving Protective clothing is mandatory for all laboratory personnel Laboratory coats must be worn in PC2 areas and left in them You should have your own labeled laboratory gown coat Laboratory coats should be removed when laboratory workers leave the work area e g at meal or toilet breaks Disposable gloves must be worn when handling all biological waste products i e quarantine deemed quarantine medical and microbiological wastes and while doing any bench laboratory work except
44. organisms must be kept at all times and must include the full scientific name the nominal risk group and the origin and storage location Appendix E is a template register to be used for that purpose including their nominal risk group origin and storage location Quarantine items are on a separate register Genetic materials are on a separate register Health Canada MSDSs for infectious organisms website www hc sc gc ca pphb dgspsp msds Risk Assessment Assignment of Risk Group Microorganisms are classified according to their level of risk This classification is based on factors such as e Pathogenicity the resulting disease incidence and severity e The route of transmission aerosol ingestion or parenteral injection e The target hosts including issues such as required infectious dose their immune status etc e The concentration of organisms in the media being handled e Agent stability its ability to survive over time or under standard disinfection regimes e The availability of effective prevention and treatment measures Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 8 Charles Darwin University There are a number of sources of categorisation information The primary reference is AS NZS 2243 3 2010 Safety in the laboratory Part 3 Microbiology Note that Risk Groups and hence containment requirements do not just depend on th
45. our personal hygiene FAILURE TO ABIDE BY THE RULES WILL RESULT IN REMOVAL OF ACCESS TO THE FACILTY Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 17 Charles Darwin University Personal Hygiene It is important that hands are washed correctly to minimise or prevent transmission of infectious agents A suggested technique is as follows e Rinse your hands in warm running water e Apply about 5 mls a squirt of antibacterial product to the palm of the hand and rub the palms together to work up lather e Using the method shown on next page wash both hands e Rinse under running water Have the hands pointing down so that water drains from the fingers into the sink This will remove the water and foam but the antibacterial soap will still be resident on the hands Then pat your hands dry and turn the tap off with the paper towel Correct Application of an Alcohol Hand Rub e Squirt onto the palm of the hand e The hands must be rubbed together vigorously ensuring the alcohol comes in contact wth ALL surfaces NOT just the palms or fingers e Pay particular attention to the tips of fingers the thumbs and the surfaces between the fingers e Continue to rub the solution until it is evaporated and the hands are dry 10 15 seconds Never wave hands to hasten drying The 10 15 seconds of rubbing is crucial Hands should be washed e Before le
46. owing areas Teaching lab Yellow 3 1 06 Tissue culture lab Yellow 2 2 34 Plant growth room Yellow 2 2 48 Biohazard lab Yellow 2 2 53 Research labs Yellow 2 2 53 amp 2 2 54 This manual will be reviewed regularly and at least annually by the Faculty Workplace Health and Safety Committee s o that it remains up to date and aligned with current legislation Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 4 Charles Darwin University Nature of Work Yellow 2 has PC2 spaces accredited by Office of the Gene Technology Regulator OGTR These spaces are also certified QC2 spaces Quarantine Containment Level 2 Work that may be undertaken approval processes apply 1 Genetic material approved by Darwin Region IBC Quarantine material approved by Technical Services Manager Pathogens of Risk Group 2 approved by Faculty IBS Human cell cultures and body fluids approved by Faculty IBS Non PC2 QC2 work must comply with these guidelines Approved by Faculty IBS Urge pac M2 Yellow 3 is a PC2 Physical Containment level 2 facility certified by the Faculty Institutional Biosafety Committee IBC Work that may be undertaken approval processes apply 1 Pathogens of Risk Group 2 approved by Faculty IBS 2 Human cell cultures and body fluids approved by Faculty IBS 3 Non PC2 QC2 work must comply with these guideli
47. ph 3 2 6 If your agent is listed in this table you must contact the Faculty Biosafety committee before bringing the agent into the University 3 Identify the containment facility level and containment controls based on the risk group of the organism or material When working with biological hazards in a laboratory animal house or any other facility it is necessary to define the level of physical containment PC 1 4 This is based on the risk group of the biohazard 4 Conduct Risk Assessment using the approved CDU forms Refer to Appendices in this Manual There are 4 different physical containment levels ranging from PC1 to PC4 Each containment level corresponds directly to the 4 risk groups of biological hazards as set out in AS NZS 2243 3 The higher the risk group rating the higher the facility containment level required and the higher the risk to laboratory workers and community The level of physical containment that is identified will dictate the facility s structural requirements containment equipment work practices and PPE requirements that are necessary to work safely with the designated risk group If it is unclear which level of physical containment is required contact your supervisor to assist you NOTE No RISK GROUP 3 or 4 organisms or material will be approved 5 Identify and obtain approval for use of the facility and the biological material through the appropriate authority e g Faculty Darwin Region IBC OGTR DAFF
48. place whenever UV lamps are in use Personnel exposed to UV radiation may suffer eye damage and erythema sunburn e UV lamps should be used for 20 to 30 minutes at the beginning and end of work They should not be left on for extended periods e UV radiation degrades nitrile plastics and rubber products and organic coatings e UV is ineffective in dynamic air streams on dried organic matter and is not penetrating Radiation intensity reduces over time due to degradation of the lamps Where the use of UV is a significant element of surface decontamination procedure regular testing of lamp intensity should be conducted This can be arranged by the Technical Services manager Testing amp Maintenance of BSC BSCs require inspection and testing of airflow and filter performance at least annually as well as after modification including filter changes or relocation Cabinets should also be tested if there is reason to suspect they are not operating correctly Testing and maintenance of biological safety cabinets is the responsibility of the Technical Service manager or delegate Users should ensure that the BSC has a current test sticker on it prior to use Records of testing are maintained in the Equipment folder Because maintenance may require access to the dirty side of the system special decontamination procedures are used to ensure maintenance personnel are protected from the work usually conducted in the BSC Consult the technical
49. rgraduate students are to notify technical staff of all issues related to pipettes DO NOT disassemble any pipettes Ergonomics and pipettes Continuous use of pipettes has the potential to result in forms of occupational overuse syndrome The following points should be observed in order to minimise the risk of this occurring Make sure the laboratory stool height is adjusted so the pipette can reach the work with the forearm and wrist held in a straight line parallel with the work surface Arrange the work to ensure that it can be reached without stretching minimise the amount of pipette travel Break from pipetting regularly at least 5 minutes every 30 minutes When carrying out large numbers of transfers use an electronic pipette in favour of a mechanical one Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 16 Charles Darwin University Administrative Controls General Laboratory Rules e 9o 10 11 12 13 14 Only authorised personnel can access the laboratory Unauthorised persons will be requested to immediately leave the facility Never give access to any person other than an authorised person Fully enclosed footwear must be worn at all times Laboratory gowns coats must be worn at all times All items must be decontaminated before they leave the laboratory Once something enters the PC2 space it cannot be removed unl
50. ritation to anaphylactic shock may be reduced by the use of non powdered and low protein latex gloves Alternatively nitrile gloves can be used which can also provide improved chemical protection Gloves shall be removed before leaving the laboratory or answering the telephone Refer to the following glove removal procedure for approved technique to be used Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 20 Charles Darwin University Instructions for the safe removal of contaminated gloves 1 Pull one glove near your wrist towards your fingertips until the glove folds over 2 Carefully grab the fold and pull towards your fingertips As you pull you are turning the inside of the glove outwards 3 Pull the fold until the glove is almost off 4 To avoid contamination continue to hold the removed glove Completely remove your hand from the glove 5 Slide your finger from your glove free hand under the remaining glove Continue to slide your finger towards your fingertips until almost half of your finger is under the glove 6 Turn you finger 180 and pull the glove outwards and towards your fingertips As you do this the first glove will be encased in the second glove The inside of the second glove will also be turned outwards 7 Grab the gloves firmly by the uncontaminated surface the side that was originally touching your h
51. rte tete ra ie ttt neds dota ae erede Rees eRe nase 17 Personal Hygiene iioii RE REMRLA RDUM RC EE I MEDI RO RUF UNE ORDIN E aS nT MICE cC EN EDU U 18 Correct Application of an Alcohol Hand RUD cceceessssececcceseesesnssecececssecseaeaecececessesesseseeeeecsssesesaeaeeeeeensees 18 Hand Washing Technique tee teet Re PER E ede Re ERR eet ea eR ntn de pa uude et uP Ups aee AEE URP acus 19 Personal Protective EquipmiehtL uossusaasscap cad ika EE Kea iba D Ra Epl MF TURA Ga CE C pA RKR RUM DERE VEM ERAI U ME 20 Clothing amp Footwear REQUuireMentts cccsessccecccecessesssseceeccececseaaaeceseceseeaaeaeceseceseeseaaeseeeesessseseaaeaeeeeesesses 20 Laboratory GOWNS ie ceca RE 20 Eve Prote ctlonicssi c2e Er 20 Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 2 Charles Darwin University GIOVBS ED RERO INE LRIENE cask I CIEN CIPUE NIU 20 Instructions for the safe removal of contaminated gloves ecessesssseceeecesseseeeaeceeseseseseaeeeeeeseesseseaeeeeess 21 Working Alone this is for research staff and students only 22 Work PrOCOQUIeS m 22 Irnioc latinpg LOO DS 5 scritte tute EMEN NU I edi 22 CAE IRI ebeteHad 22 DOC OETA NIE ARNO ic o siaetodtasudads ane ines da dxus a sain ce Gane Gece unenun
52. ry personnel are asked to verify they have received this information and may be asked to confirm that they have understood it by way of questioning and demonstration This checklist represents a record of induction and should be kept in the Induction folder Room No s Supervisor Department Item Check Review the contents of the CDU Biosafety Safety Manual Laboratory Access requirements Location of fire fighting equipment Location of first aid kit Evacuation procedures Emergency eye wash and safety shower Location of safety documents e Laboratory Safety Manual s e MSDS Folder e Equipment Folder e Risk Management Folder Personal Protective Equipment issued e Gown e Safety Glasses e Other Location of other personal protective equipment e Gloves e Respirator e Face shields Hand washing procedures explained and demonstrated Fume cupboard operation explained and demonstrated BSC operation explained and demonstrated Waste disposal procedures Other items User s Name Signature Date Inducted by Supervisor Acknowledgment Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 34 Charles Darwin University Appendix C Cleaner Maintenance Personnel Induction Checklist This is designed as an aid to inducting personnel who require access to the laboratory for the
53. s in Australia Copies of the BSC manuals for the laboratory can be found in the Equipment folder in the facility BSCs should not be confused with other laminar flow benches or fume extractions systems Class Il BSCs are open fronted ventilated containment enclosures intended for work with Risk Group 2 3 and 4 organisms that can be deactivated by a formaldehyde fumigation procedure They are self contained work stations and operate independently of all other air handling systems that may be present The cabinets incorporate High Efficiency Particulate Arresting HEPA filters which are the physical containment barrier that trap sub micron particles such as microorganisms Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 10 Charles Darwin University In Class Il BSCs an inflow of room air into a full width grille at the base of the work opening creates an air barrier A quantity of air equal to that of the barrier air normally about 3096 of the total airflow is exhausted to the room via a HEPA filter The rest is separately HEPA filtered and recirculated within the work zone via vertical downward laminar airflow to provide product protection Class Il cabinets therefore provide personnel environment and product protection However because of the exhaust into the room it is important to note that the BSC is not suitable for handling materials conta
54. services manager for testing and maintenance procedures Fume Cupboards A fume cupboard is essentially a ventilated box with an adjustable work opening that is used to minimise exposure to chemical vapours It provides extraction to remove any fumes produced within the box It is designed to have laminar flow through the front opening i e the flow is to be even and non turbulent through the open face of the cupboard Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 14 Charles Darwin University To obtain an even flow through the face of the fume cupboards baffles are generally installed at the back of the cupboard These baffles are set to extract the air from two or more heights across the back of the fume cupboard If the openings provided by the baffles are blocked by items stored in the cupboard then the air flow through the face of the cupboard can become uneven Whenever anything is placed within the fume cupboard it introduces turbulence This means that the containment of fumes may be affected If a fume cupboard is not used in the proper manner then there may be situations in which fumes escape out of the front of the fume cupboard towards the user instead of being drawn away from the user Unless the room is of sufficient size or appropriately ventilated a fume cupboard will not be able to draw sufficient air and will subsequently not function
55. tons are disabled during post purge period 5 Lower the sash to fully closed position the display will show UV MODE The sash can be lowered immediately after turning off the fan as it will not interrupt the post purge procedure 6 Turn on the UV lamp when present to decontaminate the work area by pressing the UV button 7 Leave the UV lamp on to make sure the decontamination is done effectively The UV lamp can only be turned on after the post purge procedure is finished Operating Instructions for the BSC in Yellow 2 2 34a Gelman Science Class II Cabinet Turning on the BSC 1 2 Qv ur dew Remove unnecessary items from the cabinet Run the cabinet with the UV lamp on for 10 15 minutes prior to use with the work opening cover in place Remove the work opening cover and wipe down the work zone surfaces with a suitable disinfectant Run the cabinet for at least 5 minutes so as to clear away any residual aerosols If necessary use plastic backed absorbent sheeting to reduce clean up between procedures Plan work so as to place all materials in or close to the cabinet and within easy reach of the operator Allow the cabinet to run for a further 5 minutes before use Turning off the BSC pd pO Uv w At the end of work leave the cabinet running and conduct the following procedures Transfer cultures to a container for storage or incubation Disinfect and remove all unnecessary materials to reduce the potential for cross
56. tp www daff gov au 6 Second supplement to Categorisation of biological agents according to hazard and categories of containment 4th Edition 1995 Advisory Committee on Dangerous Pathogens http www hse gov uk hthdir noframes agents htm 7 NIH Guidelines for research involving recombinant DNA molecules 2001 National Institutes of Health http www4 od nih gov oba rac guidelines guidelines html 8 The Australian Immunisation Handbook 7th Edition NHMRC 2000 9 Biosafety in Microbiological and Biomedical Laboratories BMBL 4th Edition 1999 Centre for Disease Control and Prevention amp National Institutes of Health http www cdc gov od ohs biosfty bmbl4 bmbl4toc htm Charles Darwin University Biosafety Safety Manual Version 2 0 January 2015 Note This document is uncontrolled when printed Page 29 AppendixA PC2 Laboratory Arrangement Building Yellow 2 Charles Darwin University PC2 OGTR Spaces in Yellow 2 Hand Wash Sink Legend A Fire Extinguishers A BioSafety Cabinet Fume Cupboards A Safety Showers A Eye Washes Ez First Aid Kits ONTROLLED ROOM 31 DNTROLLED T AMP ROOM STAIR f 5 zes m E Cp rage mesa i P ROOM h b IP RO E GOOM FEMALE 2 18 pa aia es PWD Due _2 18b 2 19 WO MALE 2 206 2 20 Lt A SEQUENCING ROOM 2 41 2 2 21 PC2 OGTR Areas
Download Pdf Manuals
Related Search