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Agilent 1260 Infinity Bio-inert Quaternary Pump

1. Figure 9 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 66 Bio inert Quaternary Pump User Manual Troubleshooting and Diagnostics 6 Module Status Indicator The module status indicator indicates one of six possible module conditions e When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis e A green status indicator indicates the module is performing an analysis run mode e A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running e An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis e A red blinking modules with on board LAN or yellow blinking modules without on board LAN indicator indicates that the module is in resident mode e g during update of main firmware A fast red blinking modules with on board LAN or fast yello
2. Agilent Technologies 2011 2012 Printed in Germany 05 12 G5611 90000 Rev C x Agilent Technologies
3. e e Gd Bio inert Quaternary Pump User Manual Installing the Pump Unpacking the Pump 28 Delivery Checklist 28 Accessory Kit 29 Optimizing the Stack Configuration 30 One Stack Configuration 30 Installing the Pump 33 Connecting Modules and Control Software 36 Connecting Modules 36 Connecting Control Software and or G4208 A Instant Pilot 37 Flow Connections of the Pump 38 Priming the System 41 Initial Priming 41 Regular Priming 43 Changing Solvents 44 This chapter gives information about the preferred stack setup for your system and the installation of your module te Agilent Technologies 27 3 Installing the Pump Unpacking the Pump CAUTION If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Delivery Checklist 28 Ensure all parts and materials have been delivered with the pump The delivery checklist is shown below To aid in parts identific
4. 15 Purge the system with 30 mL of solvent in order to achieve a low pressure ripple see Regular Priming on page 43 116 Bio inert Quaternary Pump User Manual Maintenance 9 Exchanging the Outlet Valve When If internally leaking Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 1924 Wrench open 14 mm Parts required p n Description G5611 60067 Outlet Valve Bio inert Preparations Switch off pump at the main power switch Remove the front cover Before exchanging the outlet valve you can try to clean it in an ultrasonicator for 5 10 min Place the valve in an upright position in a small beaker with alcohol Bio inert Quaternary Pump User Manual 117 9 Maintenance Exchanging the Outlet Valve 1 Using the 1 4 inch wrench disconnect the valve capillary from the outlet valve Using the 14 mm wrench loosen the valve and remove it from the pump body Valve capillary Outlet valve 2 Do not disassemble the outlet valve as this can damage the valve Bio inert identifier ring Plastic jacket NOTE The bio inert outlet valve can be identified by a ring which is normally covered by the plastic jacket 3 Reinstall the outlet valve and tighten the valve Reconnect the valve capillary 118 Bio inert Quaternary Pump User Manual Maintenance Exchanging the Purge Valve Frit or the Purge Valve When Tools required OR Parts require
5. Remote cables p n Description 03394 60600 Agilent module to 3396A Series integrators 3396 Series Il 3395A integrator see details in section Remote Cables on page 158 03396 61010 Agilent module to 3396 Series III 3395B integrators 5061 3378 Agilent module to Agilent 35900 A D converters or HP 1050 1046A 1049A 01046 60201 Agilent module to general purpose BCD cables p n Description 03396 60560 Agilent module to 3396 integrators G1351 81600 Agilent module to general purpose Bio inert Quaternary Pump User Manual Identifying Cables 11 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable Agilent module interface board to general purposes RS 232 cables p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m Bio inert Quaternary Pump
6. Appendix 13 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information Bio inert Quaternary Pump User Manual 185 13 Appendix Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Ud
7. 7 72 Error Information Temperature Limit Exceeded 88 Servo Restart Failed 89 Pump Head Missing 89 Index Limit 90 Index Adjustment 90 Index Missing 91 Stroke Length 91 Initialization Failed 92 Wait Timeout 93 Degasser cannot read signal 94 Degasser limit notreached 94 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions Bio inert Quaternary Pump User Manual Error Information What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook Bio inert Quaternary Pump User Manual 7 73 7 Error Information General Error Messages 74 General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well The timeout threshold was exceeded Probable cause 1 The analysis was completed successfully and the timeout function switched off the module as requested 2 Anot ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Shut Down
8. 3 Dirty or defective sensor 4 Rear of the module is exposed to strong direct sunlight Bio inert Quaternary Pump User Manual Suggested actions Reinstall the top foam Please contact your Agilent service representative Please contact your Agilent service representative Ensure that the rear of module is not directly exposed to strong sunlight 79 7 Error Information Module Error Messages 80 These errors are pump specific Restart Without Cover The module was restarted with the top cover and foam open The sensor on the main board detects when the top foam is in place If the module is restarted with the foam removed the module switches off within 30 s and the error message is generated Probable cause Suggested actions 1 The module started with the top cover and Please contact your Agilent service foam removed representative 2 Rear of the module is exposed to strong Ensure that the rear of module is not directly direct sunlight exposed to strong sunlight Solvent Zero Counter Pump firmware version A 02 32 and higher allow to set solvent bottle fillings in the data system If the volume level in the bottle falls below the specified value the error message appears when the feature is configured accordingly Probable cause Suggested actions 1 Volume in bottle below specified volume Refill bottles and reset solvent counters 2 Incorrect setting Make sure the limits are set correctly Bio in
9. Suggested actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required An external instrument has generated a shut down signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Bio inert Quaternary Pump User Manual Probable cause 3 Shut down in an external instrument with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing Remote Timeout Error Information 7 Suggested actions Check external instruments for a shut down condition Check the vacuum degasser for an error condition Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built in A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready
10. p n 01046 60201 Pin Universal Pin Agilent Signal Name Active module TTL 1 White Digital ground A Q 1 2 B ou Brown Prepare run Low 00 pees DE KEY 3 Gray Start Low oo poe 00 S EE te 4 ee Shut down Low og 5 Pink Not connected oo 26 6 Yellow Power on High 5 15 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 160 Bio inert Quaternary Pump User Manual Identifying Cables 11 BCD Cables One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 Violet 2 BCD 7 80 a Blue 3 BCD6 40 m Yellow 4 BCD 4 10 SS Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low Bio inert Quaternary Pump User Manual 161 11 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module n 1 1 BCD 5 20 ae 2 2 BCD 7 80 K 3 3 BCD 6 40 4 4 BCD 4 10 g 5 5 BCDO 1 6 6 BCD 3 8 z 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 162 Bio inert Quaternary Pump User Manual CAN L
11. the error message is generated However the pump will function as an isocratic pump in this configuration The error message reappears after each switch on Probable cause Suggested actions 1 Gradient valve disconnected Reconnect the gradient valve Bio inert Quaternary Pump User Manual 83 7 84 Error Information Valve Fuse Valve Fuse 0 Channels A and B Valve Fuse 1 Channels C and D The gradient valve in the quaternary pump has drawn excessive current causing the electronic fuse to open Probable cause 1 Defective gradient valve 2 Defective connection cable front panel to main board 3 Defective main board Inlet Valve Fuse Suggested actions Restart the quaternary pump If the error message appears again exchange the gradient valve Please contact your Agilent service representative Please contact your Agilent service representative The active inlet valve in the module has drawn excessive current causing the inlet valve electronic fuse to open Probable cause 1 Defective active inlet valve 2 Defective connection cable front panel to main board 3 Defective main board Suggested actions Restart the module If the error message appears again exchange the active inlet valve Please contact your Agilent service representative Please contact your Agilent service representative Bio inert Quaternary Pump User Manual Error Information 7 Valve Failed MCGV SSV
12. 141 Active Inlet Valve 142 Accessory Kit Bio inert G5611 68755 144 Starter Kit Bio inert 145 Solvent Cabinet 146 Bottle Head Assembly 148 Hydraulic Path 149 System Tool Kit 151 1 Identifying Cables 153 Cable Overview 154 Analog Cables 156 Remote Cables 158 BCD Cables 161 CAN LAN Cables 163 External Contact Cable 164 Agilent Module to PC 165 Agilent 1200 Module to Printer 166 12 Hardware Information 167 Electrical Connections 168 Interfaces 170 Setting the 8 bit Configuration Switch without On board LAN 13 Appendix 181 General Safety Information 182 The Waste Electrical and Electronic Equipment Directive Batteries Information 186 Radio Interference 187 Sound Emission 188 Agilent Technologies on Internet 189 Bio inert Quaternary Pump User Manual Contents 176 Contents 8 Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 1 Introduction Introduction to the Pump 10 Overview of the Hydraulic Path 11 Hydraulic Path 12 How Does the Pump Work 13 How Does Compressibility Compensation Work 15 How Does Variable Stroke Volume Work 16 Bio inert Materials 17 Early Maintenance Feedback 19 Instrument Layout 20 This chapter gives an introduction to the module instrument overview and internal connectors phe Agilent Technologies 1 introduction Introduction to the Pump Introduction to the Pump The bio inert quaternary pump comprises an op
13. 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C Bio inert Quaternary Pump User Manual Hardware Information There is no configuration possible on main boards with on board LAN These are pre configured for e 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table 10 RS 232C Connection Table 12 Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Bio inert Quaternary Pump User Manual 173 12 Hardware Information 174 Instrument PC DCD 1 1 DCD RX 2 et 2 RX TX 3 X 3 Tx DTR 4 Sf 4 DTR GND 5 y 5 GND DSR 6 6 DSR RTS 7 V 7 RTS cis 8 slias RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 19 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows eas
14. 92 Error Information Initialization Failed The module failed to initialize successfully within the maximum time window A maximum time is assigned for the complete pump initialization cycle If the time is exceeded before initialization is complete the error message is generated Probable cause Suggested actions 1 Blocked active inlet valve Exchange the active inlet valve 2 Defective pump drive assembly Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Bio inert Quaternary Pump User Manual Error Information 7 Wait Timeout When running certain tests in the diagnostics mode or other special applications the pump must wait for the pistons to reach a specific position or must wait for a certain pressure or flow to be reached Each action or state must be completed within the timeout period otherwise the error message is generated Possible Reasons for a Wait Timeout Pressure not reached Pump channel A did not reach the delivery phase Pump channel B did not reach the delivery phase Pump channel A did not reach the take in phase Pump channel B did not reach the take in phase Solvent volume not delivered within the specified time Probable cause Suggested actions 1 Purge valve open Ensure that purge valve is closed 2 Leak at fittings purge valve active inlet Ensure pump components are seated correctly valve outl
15. Information 51 Optimizing Performance 55 When to Use a Degasser 56 Operational Hints for the Multi Channel Gradient Valve MCGV 57 When to use the Seal Wash Option 58 Choosing the Right Pump Seals 59 Optimize the Compressibility Compensation Setting 60 Bio inert Quaternary Pump User Manual Contents 6 Troubleshooting and Diagnostics 63 Overview of the Module s Indicators and Test Functions 64 Status Indicators 66 User Interfaces 68 Agilent Lab Advisor Software 69 7 Error Information 71 What Are Error Messages 73 General Error Messages 74 Module Error Messages 80 8 Test Functions and Calibration 95 Introduction 96 System Pressure Test 97 Leak Rate Test 102 9 Maintenance 107 Introduction to Maintenance 108 Warnings and Cautions 109 Overview of Maintenance 111 Cleaning the Module 112 Checking and Replacing the Solvent Filter 113 Exchanging the Active Inlet Valve AIV or its Cartridge 114 Exchanging the Outlet Valve 117 Exchanging the Purge Valve Frit or the Purge Valve 119 Removing the Pump Head Assembly 122 Seal Wear in Procedure 125 Maintenance of the Pump Head 126 Reinstalling the Pump Head Assembly 129 Exchanging the Multi Channel Gradient Valve MCGV 131 Exchanging the Optional Interface Board 134 Replacing the Module s Firmware 136 Bio inert Quaternary Pump User Manual 10 Parts for Maintenance 137 Pump Head Assembly with Seal Wash Option 138 Outlet Valve 140 Purge Valve Assembly
16. PTFE frits pack of 5 5182 0716 Screw Cap Vial 2 mL amber glass write on spot 100 pk 5182 0717 Blue screw caps 100 pk 5063 6507 2x Chip Column I D Assy 5041 2168 2x Solvent inlet filter 20 um pore size G5611 68710 Capillary Fitting Starterkit ID 17 mm Bio inert Bio inert Quaternary Pump User Manual 145 10 Parts for Maintenance Solvent Cabinet Solvent Cabinet Item p n Description 1 5065 9981 Solvent cabinet including all plastic parts 2 5043 0207 Name plate 1260 3 5065 9954 Front panel solvent cabinet 4 5042 8567 Leak pan 5 9301 1420 Solvent bottle transparent 6 9301 1450 Solvent bottle amber 7 G1311 60003 Bottle head assembly 146 Bio inert Quaternary Pump User Manual Parts for Maintenance 10 Solvent Cabinet i cap Se Figure 15 Solvent Cabinet Parts Bio inert Quaternary Pump User Manual 147 10 Parts for Maintenance Bottle Head Assembly Item p n Description G1311 60003 Bottle head assembly 5063 6598 Ferrules with lock ring 10 Pk 2 5063 6599 Tube screw 10 Pk 3 Wire marker 4 5062 2483 Solvent tubing 5 m 5 5062 8517 Inlet filter adapter 4 Pk 5041 2168 Solvent inlet filter 20 um pore size Figure 16 Bottle Head Assembly Parts 148 Bio inert Quaternary Pump User Manual Hydraulic Path Parts for Maintenance 10 FM For bio inert modules use bio inert parts only Item p n 1 Aea wo N Sz Bio inert Quaternary Pump User Manual G5611 6050
17. User Manual 155 11 Identifying Cables Analog Cables 156 C BO One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module H O Shield Shield Analog E Center Center Analog Bio inert Quaternary Pump User Manual Agilent Module to General Purpose Identifying Cables 11 p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog 3 Red Analog _ ee Bio inert Quaternary Pump User Manual 157 11 Identifying Cables Remote Cables 158 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL E 9 1 White Digital ground NC 2 Brown Prepare run Low 80 7 3 3 Gray Start Low x NC 4 Blue Shut down Low oO e o gt NC 5 Pink Not connected le NC 6 Yell
18. and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative Bio inert Quaternary Pump User Manual Error Information 7 Index Missing The encoder index position in the module was not found during initialization During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is not recognized within a defined time the error message is generated Probable cause Suggested actions 1 Disconnected or defective encoder cable Please contact your Agilent service representative 2 Defective pump drive assembly Please contact your Agilent service representative Stroke Length The distance between the lower piston position and the upper mechanical stop is out of limits pump During initialization the module monitors the drive current If the piston reaches the upper mechanical stop position before expected the motor current increases as the module attempts to drive the piston beyond the mechanical stop This current increase causes the error message to be generated Probable cause Suggested actions 1 Defective pump drive assembly Please contact your Agilent service representative Bio inert Quaternary Pump User Manual 91 7
19. assembly 138 purge valve 141 solvent cabinet 146 starter kit 145 system tool kit 151 physical specifications 25 piston chamber 11 piston 13 48 power supply indicator 66 power consideration 22 power consumption 25 192 power cords 23 power switch 34 pressure above upper limit 81 pressure below lower limit 82 pressure pulsation 16 60 pressure range 59 priming withapump 43 56 with a syringe 56 proportioning valve high speed 11 PTFE frit 119 ump error messages 80 ump head assembly 122 138 ump head missing 89 ump configuration 83 ump head 126 ump piston 48 urge valve frit 48 urge valve 119 141 p p p p p p p p radio interference 187 reassembling the pump head 129 remote cable 158 removing pump head assembly 122 repairs replacing firmware 136 136 restart without cover 80 RS 232C cable 165 communication settings 177 S safety class 182 safety information lithium batteries 186 safety general information 182 standards 25 symbols 184 sapphire piston 13 seal wash option 138 sealwash 10 11 58 when to use 58 seal alternative material 59 selection valve failed 82 servo restart failed 89 shut down 74 simple repairs 108 site requirements power cords 23 snap fasteners 39 solvent inlet filters 48 solvent zero counter 80 solvent cabinet 48 146 solvent filter checking 113 replacing 113 solvent filters prevent blocking 49 solvent information 51 solvents change 44 sound emission 1
20. condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Bio inert Quaternary Pump User Manual Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation 75 7 76 Error Information Synchronization Lost Leak During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system A leak
21. formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Bio inert Quaternary Pump User Manual In This Guide In This Guide This manual covers the Agilent 1260 Infinity Bio inert Quaternary Pump G5611A 1 Introduction This chapter gives an introduction to the module instrument overview and internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Pump This chapter gives information about the preferred stack setup for your system and the installation of your module 4 Using the Pump This chapter explains the operational parameters of the module 5 Optimizing Performance This chapter gives hints on how to optimize the performance or use additional devices 6 Troubleshooting and Diagnostics This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces 7 Error Information This chapter describes
22. gasket Support ring 7 Insert the pistons and carefully press them into the seals Piston 6 Place the support rings on the piston housing pistons not installed and snap the pump head and piston housing together Note the correct position of the pin on the support ring Pump head bio inert identifier engraving Support ring Piston housing 8 Tighten the lock screw Lock screw 128 Bio inert Quaternary Pump User Manual Maintenance 9 Reinstalling the Pump Head Assembly When Tools required Parts required When reassembling the pump p n Description 8710 2392 Hexagonal key 4 0 mm 15 cm long T handle p n Description G5611 60165 Pump Head with Seal Wash Option Bio inert 79846 65501 Pump head grease 1 If needed apply a small amount of grease on the back of 2 Slide the pump head assembly onto the pump drive and the screws Normally the grease added during use a 4 mm hexagonal key to tighten the pump head manufacturing is sufficient for a long time screws stepwise with increasing torque Pump drive an J g Pump head IKAN bio inert identifier lt A engraving ji ZEN Pump head screws Bio inert Quaternary Pump User Manual 129 9 Maintenance Reinstalling the Pump Head Assembly 3 Reconnect all capillaries tubes and if installed the active inlet valve cable to its connector Outlet valve capillary Sw Active inlet valve c
23. in the degasser tubes oF O N Open the purge valve of the pump Set the flow rate to 5 mL min Select channel Al Turn the flow on Observe if the solvent in the tubing of channel A1 is advancing towards the pump If it isn t disconnect the solvent tubing from the solvent selection valve attach a syringe with a syringe adapter and pull the liquid through the degasser Reattach the tubing to the solvent selection valve Pump 30 mL isopropanol to remove residual air bubbles Switch to the next solvent channel and repeat steps 5 and 6 until all channels have been purged Turn the flow off and close the purge valve Bio inert Quaternary Pump User Manual Installing the Pump 3 Regular Priming When When the pumping system has been turned off for a certain time for example overnight air will rediffuse into the solvent channel between the vacuum degasser and the pump Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time Preparations Switch the system on The purge tool of the LabAdvisor or Instrument Utilities can be used for automatically purging the pump 1 Open the purge valve of your pump by turning it counterclockwise and set the flow rate to 5 mL min Flush the vacuum degasser and all tubes with at least 10 mL of solvent Repeat step 1 and 2 for the other channel s of the pump Set the required composition and flow rate for your app
24. on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download Bio inert Quaternary Pump User Manual 189 Index Index 8 bit configuration switch without On Board LAN 176 A accessory kit 29 144 active inlet valve cartridge 114 active inlet valve 114 142 active seal wash 10 58 adapter 114 Agilent Diagnostic software 69 Agilent Lab Advisor software 69 Agilent Lab Advisor 69 Agilent oninternet 189 algae growth 49 alternative seal material 59 ambient non operating temperature 25 ambient operating temperature 25 analog signal 1 74 analog cable 156 apgremote 1 4 AUTO mode 16 ball screw drive 13 battery safety information 186 BCD cable 161 bench space 24 bio inert 190 multi channel gradient valve bottle head assembly buffer application changing solvents Communication settings RS 232C 177 compensation sensor open 78 compensation sensor short 78 compressibility compensation 15 60 condensation 24 configuration one stack 30 connections flow 38 control software 37 D damaged parts 28 defect on arrival 28 degasser cannot read signal 94 degasser limit not reached 94 degasser operational hints 56 degasser when to use 56 Diagnostic software 69 dimensions 25 dual piston in series design 11 E electrical con
25. or EMC regulations Connecting Modules 1 Place the individual modules in a stack configuration as shown in Figure 5 on page 31 2 Ensure the power switches on the front of the modules are OFF switches stand out 3 Plug a CAN cable into the CAN connector at the rear of the respective module except vacuum degasser 4 Connect the CAN cable to the CAN connector of the next module see Figure 6 on page 32 5 Press in the power switches to turn on the modules 36 Bio inert Quaternary Pump User Manual Installing the Pump 3 Connecting Control Software and or G4208 A Instant Pilot With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN Usually the detector is producing the most data in the stack followed by the pump and it is therefore highly recommended to use either of these modules for the LAN connection 1 Ensure the power switches on the front of the modules in the stack are OFF switches stand out 2 If there are no other 1260 with LAN port in the HPLC stack install a G1369B LAN board into the extension slot of the pump 3 Connect the LAN enabled module with a LAN cable to the data system 4 Plug the CAN connector of the Instant Pilot into any available CAN port of the 1260 system 5 Plug a CAN cable into the CAN connector of the Instant Pilot 6 Connect the CAN cable to the CAN connector of one of the modules 7 Press in the power switc
26. samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the metal top cover of the module No serviceable parts inside gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides Bio inert Quaternary Pump User Manual 109 9 Maintenance CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use ac
27. the life time of the seals and the pump head parts gt Note the correct installation of the backup ring inside the support ring 126 Bio inert Quaternary Pump User Manual Maintenance 9 Maintenance of the Pump Head 1 Remove the seal holder and the seal wash support rings from the piston housing Remove the seal holder from the support ring assembly a3 es Seal holder bio inert identifier ring Seal wash support ring bio inert identifier ring Piston housing 2 Using the steel side of the insert tool remove the seal wash gasket and the secondary seal from the support ring The removed seal will be damaged and cannot be re used Seal wash support ring Secondary seal 3 Using the plastic side of the insert tool press the wash seal spring pointing upwards into the recess of the support ring 8 Insert tool Wash seal Se with titanium spring zan Support ring lt k 4 Ifthe backup seal has been removed from the seal holder insert it in the correct orientation as shown below Please ensure that the cut end faces the seal holder Seal holder Cut end D E Backup seal Bio inert Quaternary Pump User Manual 127 9 Maintenance Miaintananra nf the Pim Waad Maintenance of the Pump Head 5 Place a seal wash gasket in the recess of the support ring Put the seal holder on top of the gasket Backup seal Seal holder Seal wash
28. used for verifying the internal tightness of the pump and helps identifying parts which may have caused a leak System requirements Minimum software revisions e Lab Advisor B 01 04 SP1 G1310B 1260 Isocratic Pump G1311B 1260 Quaternary Pump G4280B 1220 Isocratic Pump G4281B 1220 Gradient Pump e Lab Advisor B 01 04 SP2 G1311C 1260 Quaternary Pump VL G4220A 1290 Binary Pump G4220B 1290 Binary Pump VL G5611A 1260 Bio inert Quaternary Pump Minimum firmware revision A 06 34 for G5611A and A 06 33 for all other pumps This test does not work in emulation mode In case of an emulated module convert to the original type first Test Principle A solvent can be chosen from available solvent channels and a maximum target pressure can be defined at which the test will be run Typically this is the maximum pressure specified for the pump The test can be run with any solvent compatible to the pump Before the test the pump is flushed with solvent in order to remove air bubbles as air bubbles are compressed during the test and therefore would appear as leaks Using a degasser is highly recommended Bio inert Quaternary Pump User Manual Test Functions and Calibration 8 pressure piston 2 at rear position piston 1 at rear position piston 2 delivers piston 1 delivers about 100 bar below target pressure 4 time Initially the pressure is increased to about 100 bar below the target press
29. valve To waste Seal Piston 1 Piston 2 104 Bio inert Quaternary Pump User Manual Test Functions and Calibration 8 Potential Causes of Leak Rate Test Failure Secondary Leak If a leak is found for movement of piston 2 secondary leak the following reasons are possible Probable cause Suggested actions 1 System not flushed properly Flush system for several minutes 2 Degassing efficiency is low Check degasser performance 3 Purge valve not closed or defect Check purge valve 4 Blank nut not installed tightly Tighten or replace blank nut 5 Outlet valve leaking read below Replace outlet valve 6 Leak at piston 2 or seal in chamber 2 Inspect piston replace piston and or seal Primary Leak If a leak is found for movement of piston 1 primary leak any leak described for piston movement 2 will cause a failure for piston 1 as well as the liquid can move through the outlet valve to chamber 2 Such cases need to be identified as described before Additionally following causes are possible Probable cause Suggested actions 1 Leak at piston 1 or seal in chamber 1 Inspect piston replace piston and or seal 2 Leak at inlet valve Replace inlet valve or inlet valve cartridge AIV only Internal Outlet Valve Leak A leak of the outlet valve will be identified separately internal outlet valve leak by calculating the difference between leak rate 1 and leak rate 2 If the second leak rate is higher than the first one this is due to a f
30. volumes result in a lower flow ripple improve composition ripple The module uses a processor controlled spindle system for driving its pistons The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume By default the stroke volume for the pump is set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended Bio inert Quaternary Pump User Manual Introduction 1 Bio inert Materials For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials in the flow path also referred to as wetted parts which are widely accepted by life scientists as they are known for optimum inertness towards biological samples and ensure best compatibility to common samples and solvents over a wide pH range Explicitly the complete flow path is free of stainless steel and free of other alloys containing metals like iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction does not contain metals at all Table 1 Bio inert Materials Module Materials Agilent 1260 Infinity Bio inert Quaternary Pump Titanium gold platinum iridium G5611A zirconium oxide ruby PTFE PEEK Agilent 1260 Infinity Bio inert High Performance Autosampler Ups
31. was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Loose or leaking purge valve inlet valve or Ensure pump components are seated correctly outlet valve If there are still signs of a leak exchange the appropriate seal purge valve inlet valve outlet valve 4 Defective pump seals Exchange the pump seals Bio inert Quaternary Pump User Manual Leak Sensor Open Error Information 7 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause 1 Leak sensor not connected to the main board 2 Defective leak sensor 3 Leak sensor incorrectly routed being pinched by a metal component Leak Sensor Short Suggested actions Please contact your Agilent service representative Please contact
32. your Agilent service representative Please contact your Agilent service representative The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause 1 Defective flow sensor 2 Leak sensor incorrectly routed being pinched by a metal component Bio inert Quaternary Pump User Manual Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative 71 7 78 Error Information Compensation Sensor Open The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Compensation Sensor Short The ambient compensation sensor NTC on the main board in the module has failed short circuit The resistance across the temperatu
33. your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 24 Bio inert Quaternary Pump User Manual Physical Specifications Site Requirements and Specifications 2 Table2 Physical Specifications Type Specification Comments Weight 14 5 kg 32 Ibs Dimensions 180 x 345 x 435 mm height x width x depth Line voltage Line frequency Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL 7 0 x 13 5 x 17 inches 100 240 VAC 10 50 or 60 Hz 5 Wide ranging capability 180 VA 110W 375 BTU 4 55 C 41 131 F 40 70 C 4 158 F lt 95 at 25 40 C 77 104 F Non condensing Up to 2000 m 6562 ft Up to 4600 m 15091 ft For storing the module Installation category II Pollution degree 2 For indoor use only Bio inert Quaternary Pump User Manual 25 2 Site Requirements and Specifications Performance Specifications 26 Table3 Specifications Agilent 1260 Infinity Bio inert Quaternary Pump G5611A Type Specifications Hydraulic system Setable flow range Recommended flow range Flow precision Flow accuracy Pressure Pressure pulsation Compres
34. 0 G5611 60502 G1311 60003 G1322 67300 G5611 67301 G5611 67300 G1311 67304 5062 2461 0100 1847 5067 4741 Description Capillary 400 x 0 17 mm titanium Bio inert pump to injector Capillary 900 x 0 17 mm titanium Bio inert pump to thermostatted autosampler Bottle head assembly Kit of 4 solvent tubes for connection degasser to MCGV including labels Capillary Chamber 1 to Damper Bio inert Capillary Damper to Chamber 2 Bio inert Connecting tube MCGV to AIV Waste tube 5 m reorder pack Adapter AIV to solvent inlet tubes ZDV union Bio inert 149 10 Parts for Maintenance 150 SS ZSA zZ DAO A A amp A A Ly 27 Figure 17 Hydraulic Flow Path Bio inert Quaternary Pump User Manual Parts for Maintenance 10 System Tool Kit HPLC System Tool Kit p n G4203 68708 p n Description 0100 1681 Adapter syringe seal wash tube 0100 1710 Mounting Tool for Tubing Connections 01018 23702 Insert tool 5023 0240 Hex driver 4 slitted 8710 0060 Hex key wrench 9 64 inch 8710 0510 2x Wrench open 1 4 5 16 inch 8710 0641 Hex key set 1 5 mm 8710 0899 Pozidriv screwdriver 8710 1534 Wrench 4 mm both ends open end 8710 1924 Wrench open 14 mm 8710 2392 Hex key 4 mm15 cm long T handle 8710 2393 Hex key 1 5 mm straight handle 10 cm 8710 2394 Hex key 9 64 inch 15 cm long T handle 8710 2409 Wrench open end 5 16 3 8 inch 8710 2411 Hex key 3 mm12 cm long 8710 241
35. 2 Hex key 2 5 mm 15 cm long straight handle 8710 2438 Hex key 2 0 mm 8710 2509 Screwdriver Torx TX8 8710 2594 Double open end wrench 4x5 9301 0411 Syringe Plastic 9301 1337 Adapter syringe solvent tube with fitting Bio inert Quaternary Pump User Manual 151 10 Parts for Maintenance Preventive Maintenance Kit yf For bio inert modules use bio inert parts only p n Description G5611 21503 Piston Seal PTFE Bio inert 2x 01018 22707 PTFE frits pack of 5 5067 4728 Seal cap 0905 1731 2x Wash Seal PTFE for Bio inert Pump 600 bar 01018 07102 Gasket Seal wash 2x 5042 8507 Seal wash pump cartridge silicone tubing 152 Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 11 Identifying Cables Cable Overview 154 Analog Cables 156 Remote Cables 158 BCD Cables 161 CAN LAN Cables 163 External Contact Cable 164 Agilent Module to PC 165 Agilent 1200 Module to Printer 166 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules oie Agilent Technologies 153 11 Identifying Cables Cable Overview 154 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n Description 35900 60750 Agilent module to 3394 6 integrators 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs
36. 3 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 Rs 232c 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Bio inert Quaternary Pump User Manual 177 12 Hardware Information 178 Table 14 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 3 4 5 0 0 9600 1 0 0 9600 0 0 1200 1 0 1 14400 0 1 2400 1 1 0 19200 0 1 4800 1 1 1 38400 Table 15 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 1 7 Bit Communication 8 Bit Communication Table 16 Parity Settings without on board LAN Switches 7 8 0 0 0 1 1 1 Parity No Parity Odd Parity Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity Bio inert Quaternary Pump User Manual Hardware Information 12 Special Settings The special settings are required for specific actions normally in a service case Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in
37. 88 special interfaces 175 special settings boot resident 179 forced cold start 179 specification physical 25 starter kit 145 status indicator 67 stroke length 91 stroke volume 13 16 synchronization lost 76 Bio inert Quaternary Pump User Manual Index system pressure test evaluating WEEE directive 185 results 99 weight 25 system pressure test 65 97 running the test 99 system setup and installation optimizing stack configuration 30 system tool kit 151 T temperature limit exceeded 88 temperature out of range 88 temperature sensor 76 test functions 64 test function introduction 96 timeout 74 troubleshooting error messages 73 64 status indicators 64 66 U unpacking the pump 28 user interfaces 68 V vacuum degasser 10 39 valve failed 85 valve frit 119 valve fuse 84 variable reluctance motor 13 variable stroke volume 16 voltage range 25 wW wait timeout 93 waste electrical and electronic equipment 185 Bio inert Quaternary Pump User Manual 193 www agilent com In This Book This manual contains technical information about the Agilent 1260 Infinity Bio inert Quaternary Pump G5611A The manual describes the following introduction e site requirements and specifications e installing the pump using the pump e optimizing performance e troubleshooting and diagnostics maintenance e parts and materials for maintenance e identifiying cables hardware information appendix
38. 9 Maintenance Exchanging the Multi Channel Gradient Valve MCGV 1 Disconnect the connecting tube waste tube and the solvent tubes from the MCGV Connection tube Solvent tubes Waste tube 2 Press the lower sides of the cover to unclip it Remove the cover 3 Disconnect the MCGV cable unscrew the two screws and remove the valve MCGV cable Screws NOTE The bio inert version of the MCGV has no specific bio label and can be identified by its part number on actuator B 132 Bio inert Quaternary Pump User Manual Maintenance 9 Exchanging the Multi Channel Gradient Valve MCGV 4 Place the new MCGV into position Make sure that channel A of the MCGV is put at the bottom right position Tighten the two screws and connect the cable to its connector MCGV cable Screws 5 Install the MCGV cover 6 Reconnect the waste funnel with the waste tube holder in the top cover Insert waste tube in the holder in the leak pan and clip tube to the MCGV cover Waste funnel yO Waste tube holder Clip 7 Reconnect the tube from the inlet valve to the middle position of the MCGV Connect solvent tubes for channels A D from the MCGV to the degasser outlets Solvent tubes Waste tube Bio inert Quaternary Pump User Manual 133 9 Maintenance Exchanging the Optional Interface Board When Board defective Parts required p n Des
39. AN Cables Identifying Cables 11 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Bio inert Quaternary Pump User Manual 163 11 Identifying Cables External Contact Cable 164 5 10 15 6 B 11 One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent Module Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 a Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected Bio inert Quaternary Pump User Manual Identifying Cables 11 Agilent Module to PC p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible
40. Agilent 1260 Infinity Bio inert Quaternary Pump User Manual Notices Agilent Technologies Inc 2011 2012 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number G5611 90000 Rev C Edition 05 12 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Shoul
41. F Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Bio inert Quaternary Pump User Manual 19 1 Introduction Instrument Layout 20 The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packag
42. G No No No Yes No No AUX G4227A Flex Cube 2 No No No No No G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED Bio inert Quaternary Pump User Manual 171 12 Hardware Information 172 The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369A B LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a connected PC with the appropriate control software If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS
43. LC applications Data above were collected from external resources and are meant as a reference Agilent cannot guarantee for the completeness and correctness of such information Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually increases at higher temperatures In case of doubt additional resources should be consulted Bio inert Quaternary Pump User Manual 53 4 Using the Pump Solvent Information 54 Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 5 Optimizing Performance When to UseaDegasser 56 Operational Hints for the Multi Channel Gradient Valve MCGV 57 When to use the Seal Wash Option 58 Choosing the Right Pump Seals 59 Optimize the Compressibility Compensation Setting 60 This chapter gives hints on how to optimize the performance or use additional devices oie Agilent Technologies 55 5 Optimizing Performance When to Use a Degasser 56 The bio inert quaternary pump has a built in degasser which should always be included to the flow path Operational Hints for the Internal Degasser If you are using the degasser for the first time if the degasser was switched off for some time for example overnight or if the degasser lines are empty you should prime the degasser before runn
44. Suggested actions 1 Pump head not installed correctly screws Install the pump head correctly Ensure nothing not secured or pump head not seated e g capillary is trapped between the pump correctly head and body 2 Broken piston Exchange the piston Bio inert Quaternary Pump User Manual 89 7 90 Error Information Index Limit The time required by the piston to reach the encoder index position was too short pump During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is reached too fast the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative Index Adjustment The encoder index position in the module is out of adjustment During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the time to reach the index position is too long the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head
45. Valve 0 Failed valve A Valve 1 Failed valve B Valve 2 Failed valve C Valve 3 Failed valve D One of the valves of the multi channel gradient valve has failed to switch correctly The processor monitors the valve voltage before and after each switching cycle If the voltages are outside expected limits the error message is generated Probable cause Suggested actions 1 Gradient valve disconnected Ensure the gradient valve is connected correctly 2 Connection cable inside instrument not Please contact your Agilent service connected representative 3 Connection cable inside instrument Please contact your Agilent service defective representative 4 Gradient valve defective Exchange the gradient valve Bio inert Quaternary Pump User Manual 85 7 Error Information Motor Drive Power The current drawn by the pump motor exceeded the maximum limit Blockages in the flow path are usually detected by the pressure sensor in the damper which result in the pump switching off when the upper pressure limit is exceeded If a blockage occurs before the damper the pressure increase cannot be detected by the pressure sensor and the module will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the module is switched off and the error message is generated Probable cause Suggested actions 1 Flow path blockage in front of the damper Ensure the capillaries and frits betwe
46. able Outlet capillary Inlet valve solvent tubing Capillary at bottom of pump head Waste tubing 4 Reinstall the front cover 130 Bio inert Quaternary Pump User Manual Maintenance 9 Exchanging the Multi Channel Gradient Valve MCGV Tools required Parts required Preparations p n Description 8710 0899 Screwdriver Pozidriv 1 p n Description G5611 67701 Multi Channel Gradient Valve MCGV Bio inert Switch off pump at the main power switch Remove the front cover Use a solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages The life time of the multi channel gradient valve can be increased by regularly flushing the valve especially when using buffers If using buffers flush all channels of the valve with water to prevent precipitation of the buffer otherwise salt crystals could drop into an unused channel and form plugs that may cause leaks of that channel Such leaks will interfere with the general performance of the valve When using buffers in combination with organic solvents in the Agilent 1260 Infinity Bio inert Quaternary Pump it is recommended to connect the aequous solutions buffers to one of the bottom ports A and D and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the buffer channel e g A buffer B organic solvent Bio inert Quaternary Pump User Manual 131
47. aking If the frit shows indication of contamination or blockage If pump performance indicates seal wear If scratched If defective Gradient performance problems intermittent pressure fluctuations Pressure ripple unstable run Leak Rate Test for verification Pressure ripple unstable run Leak Rate Test for verification Solvent dripping out of waste outlet when valve closed A pressure drop of gt 10 bar across the frit at a water flow of 5 mL min with open purge valve indicates blockage Leaks at lower pump head side unstable retention times pressure ripple unstable run Leak Rate Test for verification Seal life time shorter than usual check pistons while changing the seals Error condition indicated by red status indicator Bio inert Quaternary Pump User Manual 111 9 Maintenance Cleaning the Module The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth as liquid could drip into the module WARNING Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings 112 Bio inert Quaternary Pump User Manual Maintenance 9 Checking and Replacing the Solvent Fi
48. ate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch The power input socket accepts a line voltage of 100 240 VAC 10 witha line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Bio inert Quaternary Pump User Manual Rear View of the Module Figure 18 Rear view of the pump Bio inert Quaternary Pump User Manual Hardware Information Electrical Connections 12 Config HE Aglen Techeologios Product No 1311B MADANM Sanai No DE9xxx110 OO kg tee Techateglen AL esha Made in Germany 169 12 Hardware Information Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table9 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog optional on board APG Remot
49. ater at a rate of 5 mL min with an open purge valve When using the quaternary pump at low flow rates for example 0 2 mL min check all 1 16 inch fittings for any signs of leaks Whenever exchanging the pump seals the purge valve frit should be exchanged too When using buffers or other salt solutions flush the system with water before switching it off The seal wash option should be used when salt concentrations of 0 1 M or higher will be used for long time periods Check the pump pistons for scratches when changing the piston seals Scratched pistons will cause micro leaks and will decrease the lifetime of the seal Pressurize the system according to the wear in procedure after changing the piston seals see Maintenance of the Pump Head on page 126 Consider recommendations given in the solvent information section see Solvent Information on page 51 Bio inert Quaternary Pump User Manual Using the Pump 4 Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module e Use a sterile if possible amber solvent bottle to slow down algae growth e Filter solvents through filters or membranes
50. ation please refer to chapter Parts and Materials for Maintenance Please report missing or damaged parts to your local Agilent Technologies sales and service office Delivery Checklist p n Description G4800 64500 Agilent LC Hardware Documentation DVD G1311 60003 Bottle head assembly 4x M85550 Lab Advisor CD 5065 9981 Solvent cabinet including all plastic parts G4203 68708 HPLC System Tool Kit G5611 68707 Starter Kit Bio inert HPLC 959961 902 Column Eclipse Plus C18 4 6 x 100 mm 3 5 ym optional Bio inert Quaternary Pump User Manual Installing the Pump 3 p n Description 699975 902 Column Poroshell 120 EC C18 4 6 x 50 mm 2 7 um optional 883975 902 Column SB C18 4 6 x 150 mm 5 pm optional G1369 60002 LAN Communication Card Power cord Accessory Kit Accessory Kit Bio inert p n G5611 68755 p n Description 5062 2461 Waste tube 5 m reorder pack 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 5181 1519 CAN cable Agilent module to module 1 m G5611 60502 Capillary 900 x 0 17 mm titanium Bio inert pump to thermostatted autosampler 5042 9954 Tubing clip 2x re order 4 pk G5611 605003 Capillary 400 x 0 17 mm titanium Bio inert pump to injector Bio inert Quaternary Pump User Manual 29 3 Installing the Pump Optimizing the Stack Configuration If your module is part of a complete Agilent 1260 Infinity Liquid Chromatograph you can ensure optimum performance by installin
51. cessory units tested and approved according to the safety standards appropriate for the type of external equipment CAUTION Sample degradation and contamination of the instrument Metal parts in the flow path can interact with the bio molecules in the sample leading to sample degradation and contamination gt For bio inert applications always use dedicated bio inert parts which can be identified by the bio inert symbol or other markers described in this manual gt Do not mix bio inert and non inert modules or parts in a bio inert system 110 Bio inert Quaternary Pump User Manual Overview of Maintenance Maintenance 9 The following pages describe maintenance simple repairs of the pump that can be carried out without opening the main cover Table8 Simple Repair Procedures Procedure Typical Frequency Notes Checking and Replacing the Solvent Filter on page 113 Exchanging the Active Inlet Valve AIV or its Cartridge on page 114 Exchanging the Outlet Valve on page 117 Exchanging the Purge Valve Frit or the Purge Valve on page 119 Exchanging the Purge Valve Frit or the Purge Valve on page 119 Maintenance of the Pump Head on page 126 Exchanging pistons see Maintenance of the Pump Head on page 126 Exchanging the Optional Interface Board on page 134 If solvent filter is blocked If internally leaking If internally leaking If internally le
52. ch are widely accepted by life scientists as they are known for optimum inertness towards biological samples and ensure best compatibility to common samples and solvents over a wide pH range Explicitly the complete flow path is free of stainless steel and free of other alloys containing metals like iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction does not contain metals at all However there are no materials which combine suitability for versatile HPLC instrumentation valves capillaries springs pump heads flow cells etc with complete compatibility to all possible chemicals and application conditions This section gives recommendations which solvents should be preferred Chemicals which are known to cause issues should be avoided or exposure should be minimized e g for short term cleaning procedures After using potentially aggressive chemicals the system should be flushed with compatible standard HPLC solvents Bio inert Quaternary Pump User Manual 51 4 52 Using the Pump PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance mechanical and thermal stability and is therefore the material of choice for biochemical instrumentation It is stable in the specified pH range and inert to many common solvents There is still a number of known incompatibilities to chemicals like
53. ch cleans pistons and seals for the use of buffer solutions Bio inert Quaternary Pump User Manual 11 1 Introduction Overview of the Hydraulic Path Hydraulic Path Damper Outlet valve Pump head Purge valve MCGV Active inlet valve Figure 2 Hydraulic Path of the Bio inert Quaternary Pump 12 Bio inert Quaternary Pump User Manual Introduction 1 Vacuum chamber Degasser From solvent bottles To sampling unit and column To waste Figure 3 Hydraulic Path of the Quaternary Pump How Does the Pump Work In the pump the liquid runs from the solvent reservoir through the degasser to the MCGV and from there to the inlet valve The pump assembly comprises two substantially identical piston chamber units Both piston chamber units comprise a ball screw drive and a pump head with one sapphire piston for reciprocating movement in it A servo controlled variable reluctance motor drives the two ball screw drives in opposite directions The gears for the ball screw drives have different circumferences ratio 2 1 allowing the first piston to move at twice the speed of the second piston The solvent enters the pump head close to the bottom limit and leaves it at its top The outer diameter of the piston is smaller than the inner diameter of the pump head chamber allowing the
54. chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc When used above room temperature it is sensitive to bases and various organic solvents which can cause PEEK to swell As normal PEEK capillaries are very sensitive to high pressure especially under such conditions Agilent uses stainless steel cladded PEEK capillaries in order to keep the flow path free of steel and to ensure pressure stability to at least 600 bar In case of doubt please consult common literature about the chemical compatibility of PEEK Titanium Titanium is highly resistant to oxidizing acids e g nitric perchloric and hypochlorous acid over a wide range of concentrations and temperatures which is due to a thin oxide layer on the surface which is stabilized by oxidizing compounds Reducing acids e g hydrochloric sulfuric and phosphoric acid can slightly corrode titanium which increases with acid concentration and temperature For example the corrosion rate of 3 HCl about pH 0 1 at room temperature is about 13 um year At room temperature titanium is resistant to concentrations of about 5 sulfuric acid about pH 0 3 The addition of nitric acid to hydrochloric or sulfuric acids significantly reduces corrosion rates Titanium is subject to corrosion in anhydrous methanol wh
55. connector Disconnect the solvent inlet tube from the inlet valve be aware that solvent may leak out of the tube due to hydrostatic flow 4 Unscrew the adapter from the active inlet valve 114 Bio inert Quaternary Pump User Manual Maintenance 9 5 Using a 14 mm wrench loosen the active inlet valve and remove the valve from the pump head ae i KS y O te lt lt 7 N A Lt N gt a f lt all Ea DA L a H P AE A Dat L lt A ie a ea Bio inert identifier ring J 6 Using a pair of tweezers remove the valve cartridge from the actuator assembly 7 Before inserting the new valve cartridge clean the area in the actuator assembly Flush the cartridge area thoroughly with alcohol 8 Insert a new cartridge into the actuator assembly make sure the valve cartridge is completely inserted into the actuator assembly 9 Insert the new valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight 10 Position the valve such that the solvent inlet tube connection points towards the front 11 Using the 14 mm wrench tighten the nut by turning the valve into its final position not more than a quarter turn 12 Reconnect the adapter at the active inlet valve Bio inert Quaternary Pump User Manual 115 9 Maintenance 13 Reconnect the solvent inlet tube to the adapter Reconnect the active inlet valve cable to the connector in the Z panel 14 Reinstall the front cover
56. cription 1 G1351 68701 Interface board BCD with external contacts and BCD outputs CAUTION Electronic boards are sensitive to electrostatic discharge ESD and should be handled with care so as not to damage them Touching electronic boards and components can cause electrostatic discharge ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components 134 Bio inert Quaternary Pump User Manual Maintenance 9 Exchanging the Optional Interface Board 1 Switch off the pump at the main power switch unplug the pump from line power 2 Disconnect cables from the interface board connectors 3 Loosen the screws Slide out the interface board from the pump BCD interface board Figure 10 Exchanging the Interface Board 4 Install the new interface board Secure screws 5 Reconnect the cables to the board connector 6 Reconnect the pump to line power Bio inert Quaternary Pump User Manual 135 9 Maintenance Replacing the Module s Firmware When Tools required OR OR Parts required Preparations 136 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to
57. ctor lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces Some modules have module specific interfaces connectors They are described in the module documentation Bio inert Quaternary Pump User Manual 175 12 Hardware Information Setting the 8 bit Configuration Switch without On board LAN 176 The 8 bit configuration switch is located at the rear of the module Modules that do not have their own LAN interface e g the TCC can be controlled through the LAN interface of another module and a CAN connection to that module Figure 20 Configuration switch settings depend on configured mode All modules without on boa
58. d Preparations Bio inert Quaternary Pump User Manual Frit when piston seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across the frit at a flow rate of 5 mL min of water with purge valve opened Purge valve if internally leaking p n 8710 0510 8710 1924 p n 1 01018 22707 1 G5611 60061 1 5067 4728 Description Wrench open 1 4 5 16 inch Wrench open 14 mm Pair of tweezers Toothpick Description PTFE frits pack of 5 Purge Valve Bio inert Seal cap OPTIONAL Switch off pump at the main power switch Remove the front cover Use a solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages 119 Maintenance Exchanging the Purge Valve Frit or the Purge Valve Using a 1 4 inch wrench disconnect the pump outlet capillary from the purge valve Disconnect the waste tube Beware of leaking solvents due to hydrostatic pressure 2 Using the 14 mm wrench unscrew the purge valve and remove it 3 Remove the seal cap from the purge valve Bio inert identifier ring Valve body l PTFE frit with grove Seal cap 120 Bio inert Quaternary Pump User Manual Maintenance Reinstall the seal cap including the gold seal 6 5 Place a new frit into the purge valve with the orientation of the frit as shown below slit in frit points to the front Before reinstallation alway
59. d Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or asa commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per
60. e Special Pumps G1310B Iso Pump 2 Yes No Yes 1 G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes Yes No G1361A Prep Pump 2 Yes No Yes No Samplers G1329B ALS 2 Yes No Yes No G2260A Prep ALS G1364B FC PS 2 Yes No Yes No G1364C FC AS G1364D FC uS G1367E HiP ALS G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler G4226A ALS 2 Yes No Yes No Yes Yes Yes Yes Yes Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330B THERMOSTAT for G1330B CAN DC OUT for CAN slaves 170 Bio inert Quaternary Pump User Manual Hardware Information 12 Table9 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes G4212A B DAD 2 No Yes Yes 1 Yes G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 1 Yes G1362A RID G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with additional G1369C LAN Card G1316A C TCC 2 No No Yes No Yes G1322A DEG No No No No No Yes AUX G1379B DE
61. e is leaking If the pump is determined to be the source of the leak run the Pump Leak Rate Test Bio inert Quaternary Pump User Manual System Pressure Test failed Test Functions and Calibration 8 The test will fail if the sum of all leaks in the system pump autosampler or column compartment and connections exceeds the test limit After isolating and fixing the cause of the leak repeat the System Pressure Test to confirm the system is pressure tight Probable cause 1 Purge valve open 2 Loose or leaky fittings 3 Pump Damaged pump seals or pistons 4 Loose purge valve 5 Autosampler Loose or leaky fitting 6 Autosampler Rotor seal injection valve 7 Autosampler Damaged metering seal or piston 8 Autosampler Needle seat 9 Column compartment Loose or leaky fitting 10 Column compartment Rotor seal in optional valve Bio inert Quaternary Pump User Manual Suggested actions Close the purge valve Tighten the fitting or exchange the capillary Run the Leak Rate Test to confirm the leak Tighten the purge valve nut 14 mm wrench Tighten or exchange the fitting or capillary Exchange the rotor seal Exchange the metering seal Check the piston for scratches Exchange the piston if required Exchange the needle seat Tighten or exchange the fitting or capillary Exchange the rotor seal 101 Test Functions and Calibration Leak Rate Test 102 Introduction The Leak Rate Test is
62. ease contact your Agilent service representative 88 Bio inert Quaternary Pump User Manual Error Information 7 Servo Restart Failed The pump motor in the module was unable to move into the correct position for restarting When the module is switched on the first step is to switch on the C phase of the variable reluctance motor The rotor should move to one of the C positions The C position is required for the servo to be able to take control of the phase sequencing with the commutator If the rotor is unable to move or if the C position cannot be reached the error message is generated Probable cause Suggested actions 1 Disconnected or defective cable Please contact your Agilent service representative 2 Blocked active inlet valve Exchange the active inlet valve 3 Mechanical blockage of the module Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly 4 Defective pump drive assembly Please contact your Agilent service representative 5 Defective main board Please contact your Agilent service representative Pump Head Missing The pump head end stop in the pump was not found When the pump restarts the metering drive moves forward to the mechanical end stop Normally the end stop is reached within 20 seconds indicated by an increase in motor current If the end point is not found within 20 seconds the error message is generated Probable cause
63. ed Bio inert Quaternary Pump User Manual Optimizing Performance 5 Table5 Solvent Compressibility Solvent pure Compressibility 10 6 bar Acetone 126 Acetonitrile 115 Benzene 95 Carbon tetrachloride 110 Chloroform 100 Cyclohexane 118 Ethanol 114 Ethyl acetate 104 Heptane 120 Hexane 150 Isobutanol 100 lsopropanol 100 Methanol 120 1 Propanol 100 Toluene 87 Water 46 Bio inert Quaternary Pump User Manual 61 5 Optimizing Performance Optimize the Compressibility Compensation Setting 62 Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 64 Status Indicators 66 Power Supply Indicator 66 Module Status Indicator 67 User Interfaces 68 Agilent Lab Advisor Software 69 This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces phe Agilent Technologies 63 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each mes
64. ed for most applications However applications that use normal phase solvents for example hexane are not suited for the standard seal and require a different seal when used for a longer time in the pump For applications that use normal phase solvents for example hexane we recommend using bio inert PE piston seals and bio inert PE wash seals For normal phase applications these seals have less abrasion compared to the standard seals Polyethylene seals have a limited pressure range of 0 200 bar When used above 200 bar their lifetime is reduced significantly DO NOT apply the seal wear in procedure performed with new standard seals at 600 bar Bio inert Quaternary Pump User Manual 59 5 Optimizing Performance Optimize the Compressibility Compensation Setting 60 The compressibility compensation default setting is 100 x 10 bar for the pump This setting represents an average value Under normal conditions the default setting typically reduces the pressure pulsation to values below 1 of system pressure that are sufficient for most applications and for all gradient analyses The compressibility settings can be optimized by using the values for the various solvents described in Table 5 on page 61 If the solvent in use is not listed in the compressibility tables when using isocratic mixtures of solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the com
65. ems When using salt solutions and organic solvents it is recommended to connect the salt solution to one of the bottom ports of the MCGV and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the salt solution channel Regular flushing with water of all MCGV channels is recommended to remove all possible salt deposits in the valve ports Bio inert Quaternary Pump User Manual 57 5 Optimizing Performance When to use the Seal Wash Option 58 Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent The seal wash option is strongly recommended when buffer concentrations of 0 1 M or higher will be used for long time periods in the pump The seal wash option comprises a support ring secondary seal gasket and seal holder for both piston sides Place a wash bottle filled with 90 water 10 isopropanol above the pump in the solvent cabinet The peristaltic pump moves a flow through the pump head removing all possible buffer crystals from the back of the pump seal This mixture prevents growth of algae or bacteria in the wash bottle and reduces the surface tension of the water Bio inert Quaternary Pump User Manual Optimizing Performance 5 Choosing the Right Pump Seals The standard seal for the pump can be us
66. en the pump head and damper inlet are free from blockage 2 Blocked active inlet valve Exchange the active inlet valve 3 Blocked outlet valve Exchange the outlet valve 4 High friction partial mechanical blockage in Remove the pump head assembly Ensure there the pump drive assembly is no mechanical blockage of the pump head assembly or pump drive assembly 5 Defective pump drive assembly Please contact your Agilent service representative 6 Defective main board Please contact your Agilent service representative 86 Bio inert Quaternary Pump User Manual Encoder Missing Error Information 7 The optical encoder on the pump motor in the module is missing or defective The processor checks the presence of the pump encoder connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable cause 1 Defective or disconnected pump encoder connector 2 Defective pump drive assembly Inlet Valve Missing Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative The active inlet valve in the module is missing or defective The processor checks the presence of the active inlet valve connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable cause 1 Disconnected or defective cable 2 Disconnected or defective connection cable front
67. er it is likely that the protection has been impaired Bio inert Quaternary Pump User Manual Appendix 13 the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing
68. ert Quaternary Pump User Manual Pressure Above Upper Limit Error Information The system pressure has exceeded the upper pressure limit Probable cause 1 Upper pressure limit set too low 2 Blockage in the flowpath after the damper 3 Defective damper 4 Defective main board Bio inert Quaternary Pump User Manual Suggested actions Ensure the upper pressure limit is set to a value suitable for the analysis Check for blockage in the flowpath The following components are particularly subject to blockage inline filter frit needle autosampler seat capillary autosampler sample loop autosampler column frits and capillaries with small internal diameters e g 50 um ID Please contact your Agilent service representative Please contact your Agilent service representative 7 81 7 82 Error Information Pressure Below Lower Limit The system pressure has fallen below the lower pressure limit Probable cause Suggested actions 1 Lower pressure limit set too high Ensure the lower pressure limit is set to a value suitable for the analysis 2 Air bubbles in the mobile phase Ensure solvents are degassed Purge the module Ensure solvent inlet filters are not blocked 3 Leak Inspect the pump head capillaries and fittings for signs of a leak Purge the module Run a pressure test to determine whether the seals or other module components are defective 4 Defective damper Please c
69. et valve or piston seals If there are still signs of a leak exchange the appropriate seal purge valve active inlet valve outlet valve piston seal 3 Flow changed after starting test Ensure correct operating condition for the special application in use 4 Defective pump drive assembly Please contact your Agilent service representative Bio inert Quaternary Pump User Manual 93 7 94 Error Information Degasser cannot read signal The pump board gets no or wrong pressure signals from the built in degasser Probable cause 1 Degasser board defect missing or not connected to the pump main board 2 Degasser sensor defect or not connected to degasser board Degasser limit not reached Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative This error is thrown if the degasser does not become ready after 8 min i e is higher than 180 mbar Probable cause 1 Liquid in degasser tubing 2 Leak in degasser tubing or chamber 3 Degasser vacuum pump defect Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 8 Test Functions and Calibration Introduction 96 System Pressure Test 97 Running the Test 99 Evaluating the Resul
70. flow path this problem is less common How to Prevent and or Reduce the Algae Problem Always use freshly prepared solvents especially use demineralized water which was filtered through about 0 2 um filters Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle Solvent bottle amber p n 9301 1450 supplied with the instrument for your aqueous mobile phase If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase Bio inert Quaternary Pump User Manual Using the Pump 4 Solvent Information Solvent Information Observe the following recommendations on the use of solvents e Follow recommendations for avoiding the growth of algae see Algae Growth in HPLC Systems on page 50 Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Avoid or minimize the use of solvents which may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Solvent information for parts of the 1260 Infinity Bio inert LC system For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials see Bio inert Materials on page 17 in the flow path also referred to as wetted parts whi
71. formation on safety legal and web ee Agilent Technologies 181 13 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation 182 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenev
72. frequency range 25 G general error messages 74 gradient valve 131 H hints for successful use 48 humidity 25 hydraulic path 149 index limit 90 index adjustment 90 index missing 91 initialization failed 92 initialization 15 inlet valve fuse 84 inlet valve missing 87 installation pump module 33 Bio inert Quaternary Pump User Manual installation bench space 24 instrument layout 20 interface cables 35 interfaces 170 internet 189 introduction to the pump 10 L LAN cable 163 leak rate test 102 running the test 104 leak rate test evaluating results 104 leak sensor open 77 leak sensor short 77 leak 76 line frequency 25 line voltage 25 lithium batteries 186 M maintenance feedback 19 overview 111 pump head 126 replacing firmware 136 136 materials bio inert 17 message ignition without cover 79 79 remote timeout 75 missing pressure reading 83 missing parts 28 motor drive power 86 m ulti channel gradient valve MCGV 131 191 Index non operating altitude 25 non operating temperature 25 0 operating Altitude 25 operating temperature 25 operational hints MCGV 57 optimization stack configuration 30 optional interface board 134 outlet valve 117 140 overview pump 11 P packaging damaged 28 parts identification cables 153 parts accessory kit 144 active inlet valve 142 bottle head assembly 148 damaged 28 hydraulic path 149 missing 28 outlet valve 140 pump head
73. g the following configurations These configurations optimize the system flow path ensuring minimum delay volume One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1260 Infinity LC System in the following configuration see Figure 5 on page 31 and Figure 6 on page 32 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required 30 Bio inert Quaternary Pump User Manual Installing the Pump 3 Optimizing the Stack Configuration Solvent cabinet oe e Pump S 900 000 888 Local user interface oe p Autosampler Thermostatted column compartment Detector Figure 5 Recommended Stack Configuration Front View Bio inert Quaternary Pump User Manual 31 3 Installing the Pump Optimizing the Stack Configuration AC power CAN bus cable to local user interface Remote cable CAN bus cable LAN to control software location depends on detector EEEEELLJ Analog detector signal 1 or 2 outputs per detector Figure 6 Recommended Stack Configuration Rear View 32 Bio inert Quaternary Pump User Manual Installing the Pump 3 Installing the Pump Parts required Preparations p n Description 1 Pump 1 Data System 1 G4208A Instant Pilot 1 Power cord For other cables see text below a
74. gae Growth in HPLC Systems 50 How to Prevent and or Reduce the Algae Problem 50 Solvent Information 51 This chapter explains the operational parameters of the module App Agilent Technologies 47 4 Using the Pump Hints for Successful Use of the Quaternary Pump 48 Always place the solvent cabinet with the solvent bottles on top of the quaternary pump or at a higher level When using salt solutions and organic solvents in the quaternary pump it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the salt solution channel Regular flushing of all MCGV channels with water is recommended to remove all possible salt deposits in the valve ports Before operating the quaternary pump flush the pump and vacuum degasser see Regular Priming on page 43 This is especially recommended if it has been turned off for some time for example overnight and volatile solvent mixtures are used in the channels Prevent blocking of solvent inlet filters Never use the pump without solvent inlet filter Prevent the growth of algae see Prevent Blocking of Solvent Filters on page 49 Regularly check the purge valve frit and column frit A blocked purge valve frit can be identified by a black or yellow surface deposits or by a pressure greater than 10 bar when pumping distilled w
75. hes to turn on the modules The Agilent control software can also be connected to the system through a LAN cable which requires the installation of a LAN board For more information about connecting the Instant Pilot or Agilent control software refer to the respective user manual For connecting the Agilent 1260 Infinity equipment to non Agilent 1260 Infinity equipment see Introduction to the Pump on page 10 Bio inert Quaternary Pump User Manual 37 3 Installing the Pump Flow Connections of the Pump Parts required Description 1 Other modules 1 Parts from accessory kit 2 wrenches 1 4 5 16 inch for capillary connections Preparations Pump is installed in the LC system WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere 38 Bio inert Quaternary Pump User Manual Installing the Pump 3 1 Remove the front cover by pressing the snap fasteners on both sides N Place the solvent cabinet on t
76. ich can be avoided by adding a small amount of water about 3 Slight corrosion is possible with ammonia gt 10 Fused silica Fused silica is inert against all common solvents and acids except hydrofluoric acid It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH larger than 12 the use of flow cells with sapphire windows is recommended Bio inert Quaternary Pump User Manual Using the Pump 4 Gold Gold is inert against all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia a mixture of concentrated hydrochloric and nitric acid Zirconium Oxide Zirconium Oxide ZrOg is inert against almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert against almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications PTFE PTFE polytetrafluorethen is inert against almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Sapphire Ruby and Al 03 based ceramics Sapphire Ruby and ceramics based on AlgO3 are inert against almost all common acids bases and solvents There are no documented incompatibilities for HP
77. ing an analysis The degasser can be primed either by drawing solvent through the degasser with a syringe or by pumping with the pump Priming the degasser with a syringe is recommended when e degasser is used for the first time or solvent tubes are empty or e changing to solvents that are immiscible with the solvent currently in the vacuum tubes Priming the degasser by using the pump at high flow rate 3 5 mL min is recommended when pump was turned off for some time for example during night and volatile solvent mixtures are used or e solvents have been changed Bio inert Quaternary Pump User Manual Optimizing Performance 5 Operational Hints for the Multi Channel Gradient Valve MCGV In a mixture of salt solutions and organic solvent the salt solution might be well dissolved in the organic solvent without showing precipitations However in the mixing point of the gradient valve at the boundary between the two solvents micro precipitation is possible Gravity forces the salt particles to fall down Normally the A channel of the valve is used for the aqueous salt solution and the B channel of the pump is used for the organic solvent If used in this configuration the salt will fall back into the aqueous solution and will be dissolved When using the pump in a different configuration e g D salt solution A organic solvent the salt can fall into the port of the organic solvent and may lead to performance probl
78. ing technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 2 Site Requirements and Specifications Site Requirements 22 Physical Specifications 25 Performance Specifications 26 This chapter provides information on environmental requirements physical and performance specifications wit Agilent Technologies 21 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 2 on page 25 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Hazard of electrical shock or damage of your instrumen
79. ing the Pump 3 Installing the Pump 4 Connect the required interface cables to the quaternary pump see Connecting Modules and Control Software on page 36 Configuration switch Slot for interface board T K lso oS E CAN CAN Analog pressure APG remote RS 232C 3D Asie Technologies Product No 13418 Seat OB Vaxxt1 Power 5 Connect all capillaries solvent tubes and waste tubing see Flow Connections of the Pump on page 38 6 Press the power switch to turn on the module NOTE The power switch stays pressed in and a green indicator lamp in the power switch is on when the module is turned on When the line power switch stands out and the green light is off the module is turned off 7 Purge the quaternary pump see Initial Priming on page 41 NOTE The pump was shipped with default configuration settings To change these settings see Setting the 8 bit Configuration Switch without On board LAN on page 176 Bio inert Quaternary Pump User Manual 35 3 Installing the Pump Connecting Modules and Control Software WARNING Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety
80. keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third part control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Diagnostic Software Instant Pilot G4208A Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 10 Parts for Maintenance Pump Head Assembly with Seal Wash Option 138 Outlet Valve 140 Purge Valve Assembly 141 Active Inlet Valve 142 Accessory Kit Bio inert G5611 68755 144 Starter Kit Bio inert 145 Solvent Cabinet 146 Bottle Head Assembly 148 Hydraulic Path 149 System Tool Kit 151 This chapter provides information on parts for maintenance App Agilent Technologies 137 10 Parts for Maintenance Pump Head Assembly with Seal Wash Option 138 BIO Linert For bio inert modules use bio inert parts onl
81. lication and close the purge valve 5 Pump for approximately 10 minutes before starting your application Bio inert Quaternary Pump User Manual 43 3 Installing the Pump Changing Solvents When Parts required Preparations CAUTION When the solvent of a channel is to be replaced by another solvent that is not compatible solvents are immiscible or one solvent contains a buffer it is necessary to follow the procedure below to prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system p n Description 1 Purging solvent s see Table 4 on page 45 1 5067 4741 ZDV union Bio inert Remove the column and replace it by a ZDV fitting Prepare bottles with appropriate intermediate solvents see Table 4 on page 45 1 Ifthe channel is not filled with buffer proceed to step 4 Place the solvent intake filter into a bottle of water Flush the channel at a flow rate suitable for the installed tubing typically 3 5 mL min for 10 min 4 Modify the flow path of your system as required for your application For delay volume optimization see the Rapid Resolution System manual Buffer salt of aqueous buffers may precipitate in residual isopropanol Capillaries and filter may be cloged by precipitating salt gt Don t perform steps 5 to 7 for channels run with aqueous buffer as solvent 44 Replace the solvent bottle by a bottle of isopropanol Flush the channel at a flow rate sui
82. low back through the outlet valve Probable cause Suggested actions 1 Leak at outlet valve Replace the part which has failed and re run the test Bio inert Quaternary Pump User Manual 105 8 Test Functions and Calibration Leak Rate Test 106 Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 9 Maintenance Introduction to Maintenance 108 Warnings and Cautions 109 Overview of Maintenance 111 Cleaning the Module 112 Checking and Replacing the Solvent Filter 113 Exchanging the Active Inlet Valve AIV or its Cartridge 114 Exchanging the Outlet Valve 117 Exchanging the Purge Valve Frit or the Purge Valve 119 Removing the Pump Head Assembly 122 Seal Wear in Procedure 125 Maintenance of the Pump Head 126 Reinstalling the Pump Head Assembly 129 Exchanging the Multi Channel Gradient Valve MCGV 131 Exchanging the Optional Interface Board 134 Replacing the Module s Firmware 136 This chapter describes the maintenance of the module oie Agilent Technologies 107 9 Maintenance Introduction to Maintenance The module is designed for easy repair The most frequent repairs such as piston seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack 108 Bio inert Quaternary Pump User Manual Maintenance 9 Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents
83. lter When Parts required CAUTION A functional solvent filter is essential for a good pump performance and for protecting the LC system If solvent filter is blocked p n Description 5041 2168 Solvent inlet filter 20 um pore size See Bottle Head Assembly on page 148 for related parts Small particles can permanently block the capillaries and valves of the module Damage of the module gt Always filter solvents gt Never use the module without solvent inlet filter If the filter is in good condition the solvent will freely drip out of the solvent tube hydrostatic pressure If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube 1 Remove the solvent filter from the inlet filter adapter and replace it by a new one Bio inert Quaternary Pump User Manual 113 9 Maintenance Exchanging the Active Inlet Valve AIV or its Cartridge When If internally leaking backflow Tools required Description Wrench 14mm Pair of tweezers Parts required p n Description G5611 60025 Active Inlet Valve AIV Bio inert without cartridge G5611 60020 Cartridge for AIV Bio inert G1311 67304 Connecting tube MCGV to AIV Preparations Switch off pump at the main power switch and unplug the power cable Use a solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages 1 Remove the front cover Unplug the active inlet valve cable from the
84. maximum pressure that is compatible to your system For example do not include columns a standard pressure flow cell up to 20 bar or a 400 bar autosampler to a 600 bar pressure test Running the test from the Agilent Lab Advisor 1 Select the System Pressure Test from the Test Selection menu 2 Start the test and follow the instructions Make sure to release the pressure by slowly opening the purge valve when the test has finished Evaluating the Results on page 99 describes the evaluation and interpretation of the System Pressure Test results For detailed instructions refer to the Agilent Lab Advisor software Evaluating the Results The test fails if the leak rate between pump and blank nut is higher than the limit of 3 uL min If the System Pressure Test fails e Ensure that all fittings between the pump and the blank nut are tight e Repeat the test Bio inert Quaternary Pump User Manual 99 100 Test Functions and Calibration Often it is only a damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened If the test fails again insert the blank nut at the outlet of the previous module in the stack e g autosampler outlet if TCC has been tested before and repeat the test Exclude each module one by one to determine which modul
85. nd Cable Overview on page 154 Locate bench space Provide power connections Unpack the module Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Bio inert Quaternary Pump User Manual 33 3 Installing the Pump Installing the Pump 1 Place the module on the bench in a horizontal position 2 Ensure the power switch on the front of the module is OFF switch stands out Status lamp ik Name plate O Power switch Serial number Figure 7 Front of Pump 3 Connect the power cable to the power connector at the rear of the module 34 Bio inert Quaternary Pump User Manual Install
86. nections descriptions of 168 electronic waste 185 electrostatic discharge ESD 134 EMF early maintenance feedback 19 encoder missing 87 error messages degasser cannot read signal 94 error messages degasser limit not reached 94 Bio inert Quaternary Pump User Manual Index error messages pump error 80 error messages compensation sensor open 78 compensation sensor short 78 encoder missing 87 fan failed 79 ignition without cover 79 79 index adjustment 90 index limit 90 index missing 91 initialization failed 92 inlet valve fuse 84 inlet valve missing 87 eak sensor open 77 eak sensor short 77 eak 76 missing pressure reading 83 motor drive power 86 pressure above upper limit 81 pressure below lower limit 82 pump configuration 83 pump head missing 89 remote timeout 75 selection valve failed 82 servo restart failed 89 shut down 74 solvent zero counter 80 stroke length 91 synchronization lost 76 temperature limit exceeded 88 temperature out of range 88 timeout 74 valve failed 85 valve fuse 84 wait timeout 93 exchanging active inlet valve 114 active inlet valve cartridge 114 inlet valve 111 multi channel gradient valve MCGV 131 optional interface board 134 outlet valve 117 111 pistons 111 pump seals 111 purge valve frit 119 111 purge valve 119 111 external contact cable 164 F fan failed 79 firmware updates 136 136 upgade downgrade 136 upgrade downgrade 136 flow connections 38
87. nnect the solvent tubing from the inlet valve 5 Remove the capillary at the bottom of the pump head Bio inert Quaternary Pump User Manual 123 9 Maintenance 6 Using a 4 mm hexagonal key stepwise loosen the two pump head screws and remove the pump head from the pump drive r Pump head screws TO A 124 Bio inert Quaternary Pump User Manual Maintenance 9 Seal Wear in Procedure Parts required CAUTION p n Description 0100 1847 Adapter AIV to solvent inlet tubes 5022 2159 Restriction capillary Seal damage gt This procedure is required for Standard seals pack of 2 p n 5063 6589 but it will damage the PE seals The stainless steel restriction capillary is acceptable for a temporary test within a bio inert system In case of concerns a PEEK capillary e g PEEK capillary 150 cm length 0 13 mm i d p n 0890 1915 and PEEK fittings e g PEEK Fittings 10 PK p n 5063 6591 may be used instead 1 Place a bottle with 100 mL of isopropanol in the solvent cabinet and put a tubing including bottle head assembly into the bottle 2 If an active inlet valve is installed screw the PEEK adapter 1 4 28 to 10 32 p n 0100 1847 to the AIV and connect the inlet tube from the bottle head directly to it 3 Connect the Restriction capillary p n 5022 2159 to the purge valve Connect its other end to a waste container 4 Open the purge valve and purge the system for 5 minutes wi
88. ontact your Agilent service representative 5 Defective main board Please contact your Agilent service representative 6 Defective active inlet valve AIV Replace AIV cartridge Pressure Signal Missing The pressure signal of the damper is missing The pressure signal of the damper must be within a specific voltage range If the pressure signal is missing the processor detects a voltage of approximately 120mV across the damper connector Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative Bio inert Quaternary Pump User Manual Error Information 7 Missing Pressure Reading The pressure readings read by the pump ADC analog digital converter are missing The ADC reads the pressure signal of from the damper every Ims If the readings are missing for longer than 10 seconds the error message is generated Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Pump Configuration At switch on the quaternary pump has recognized a new pump configuration The quaternary pump is assigned its configuration at the factory If the gradient valve is disconnected and the quaternary pump is rebooted
89. op of the quaternary pump wo Put the bottle head assemblies into solvent reservoirs containing your mobile phase and place the bottle in the solvent cabinet 4 Connect the inlet tubes from the bottle head assemblies to the inlet connectors A to D typically the left connection of the channel of the vacuum degasser Fix the tubes in the tube clips of the pump 5 Connect the solvent tubes to the outlet connectors typically right connection of the channel of the vacuum degasser 6 Using a piece of sanding paper connect the waste tubing to the purge valve and place it into your waste system 7 Ifthe pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system 8 Connect the pump outlet capillary pump to injection device to the outlet of the purge valve Bio inert Quaternary Pump User Manual 39 3 Installing the Pump Flow Connections of the Pump 9 Prime your system before first use see Initial Priming on page 41 Bottle head assembly Tubings Solvent cabinet Outlet Purge valve Waste tubing Outlet capillary to autosampler Figure 8 Flow Connections of the Pump 40 Bio inert Quaternary Pump User Manual Installing the Pump 3 Priming the System Initial Priming When Parts required Preparations Before a new degasser or new
90. ow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators p n 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready Bio inert Quaternary Pump User Manual Identifying Cables 11 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low NC 4 Blue Shut down Low e o NC 5 Pink Not connected le NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 A D Pin Agilent Signal Name Active module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Bio inert Quaternary Pump User Manual 159 11 Identifying Cables Agilent Module to General Purpose
91. panel to main board 3 Defective active inlet valve Bio inert Quaternary Pump User Manual Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Exchange the active inlet valve 87 7 Error Information Temperature Out of Range The temperature sensor readings in the motor drive circuit are out of range The values supplied to the ADC by the hybrid sensors must be between 0 5 V and 4 3 V If the values are outside this range the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Temperature Limit Exceeded The temperature of one of the motor drive circuits is too high The processor continually monitors the temperature of the drive circuits on the main board If excessive current is being drawn for long periods the temperature of the circuits increases If the temperature exceeds the upper limit the error message is generated Probable cause Suggested actions 1 High friction partial mechanical blockage in Remove the pump head assembly Ensure there the pump drive assembly is no mechanical blockage of the pump head assembly or pump drive assembly 2 Partial blockage of the flowpath in front of Ensure the outlet valve is not blocked the damper 3 Defective pump drive assembly Please contact your Agilent service representative 4 Defective main board Pl
92. pressibility settings When using mixtures of solvents it is not possible to calculate the compressibility of the mixture by interpolating the compressibility values of the pure solvents used in that mixture or by applying any other calculation In these cases the following empirical procedure has to be applied to optimize your compressibility setting Inappropriate settings would mainly affect retention times of peaks eluted at the beginning of a gradient Therefore optimize settings for the solvent at the beginning of the gradient For mixtures containing up to 50 water use compressibility settings of water Start the pump with the required flow rate 2 Before starting the optimization procedure the flow must be stable Check the tightness of the system with the pressure test 3 Your pump must be connected to a data system or Instant Pilot with which the pressure and ripple can be monitored or connect an external measurement device to the analog pressure output see Electrical Connections on page 168 4 Starting with a compressibility setting of 10 x 10 bar increase the value in steps of 10 Re zero the signal display as required The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition If ChemStation classic view is used and the ripple shown is positive then the compressibility setting should be decreased If it is negative it should be increas
93. rd LAN e default should be ALL DIPS DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 e DIP 1 DOWN and DIP 2 UP allows special RS 232 settings e for boot test modes DIPS 1 2 must be UP plus required mode For normal operation use the default best settings Switch settings provide configuration parameters for serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN Bio inert Quaternary Pump User Manual Hardware Information 12 The following tables represent the configuration switch settings for the modules without on board LAN only Table 12 8 bit Configuration Switch without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 RSVD SYS RSVD RSVD FC The LAN settings are done on the LAN Interface Card G1369A B Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 1
94. re compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Bio inert Quaternary Pump User Manual Fan Failed Error Information 7 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Probable cause 1 Fan cable disconnected 2 Defective fan 3 Defective main board 4 Improperly positioned cables or wires obstructing fan blades Open Cover The top foam has been removed Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Ensure the fan is not mechanically blocked The sensor on the main board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable cause 1 The top foam was removed during operation 2 Foam not activating the sensor
95. s check the gold seal in the seal cap A deformed seal cap should be exchanged Insert the purge valve into the pump head and locate the pump outlet capillary and the waste tube tf FA Outlet capillary Purge valve Waste tube 7 Tighten the purge valve and reconnect outlet capillary and waste tubing Bio inert Quaternary Pump User Manual 121 9 Maintenance Removing the Pump Head Assembly When Exchanging the seals Exchanging the pistons Exchanging seals of the seal wash option Tools required p n Description Wrench 1 4 inch 8710 2392 Hexagonal key 4 0 mm 15 cm long T handle Preparations Switch off pump at the main power switch and unplug the power cable Use a solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages CAUTION Damage of the pump drive Starting the pump when the pump head is removed may damage the pump drive gt Never start the pump when the pump head is removed 122 Bio inert Quaternary Pump User Manual Maintenance Removing the Pump Head Assembly 1 Remove the front cover by pressing the clip fasteners on both sides of the cover 2 Disconnect the active inlet valve cable 3 Using a 1 4 inch wrench remove the outlet capillary Outlet valve capillary Outlet capillary Capillary at bottom of pump head Waste tubing Inlet valve solvent tubing 4 Remove the waste tubing and disco
96. s upwards delivering to the system After a controller defined stroke length that depends on the flow rate the drive motor is stopped and the active inlet valve closes The motor direction is reversed and moves the first piston up until it reaches the stored upper limit and at the same time the second piston moves downwards Then the sequence starts again moving the pistons up and down between the two limits During the up movement of the first piston the solvent in the pump chamber is pressed through the outlet valve into the second pump chamber The second piston draws in half of the volume displaced by the first piston and the remaining half volume is directly delivered to the system During the drawing stroke of the first piston the second piston delivers the drawn volume to the system For solvent compositions from the solvent bottles A B C D the controller divides the length of the intake stroke in certain fractions in which the gradient valve connects the specified solvent channel to the pump input For specifications of the quaternary pump see Performance Specifications on page 26 How Does Compressibility Compensation Work The compressibility of the solvents in use will affect retention time stability when the back pressure in the system changes for example ageing of column In order to minimize this effect the pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent
97. sage a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Tests and Calibrations 64 Bio inert Quaternary Pump User Manual Troubleshooting and Diagnostics 6 System Pressure Test The System Pressure Test is a quick test designed to determine the pressure tightness of the system i e the high pressure flow path between pump and column After exchanging flow path components e g pump seals or injection seal use this test to verify the system is pressure tight see System Pressure Test on page 97 Leak Rate Test The Leak Rate Test is a diagnostic test designed to determine the pressure tightness of the pump components When a problem with the pump is suspected use this test to help troubleshoot the pump and its pumping performance see Leak Rate Test on page 102 Bio inert Quaternary Pump User Manual 65 6 Troubleshooting and Diagnostics Status Indicators Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status indicator iglieat Dochociogioe 1297 haliatty SS a nF Power supply indicator
98. serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used Bio inert Quaternary Pump User Manual 183 13 Appendix Safety Symbols Table 19 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the A instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced G by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up WARNING es alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions A CAUTION alerts you to situations that could cause loss of data or damage of equipment CAUTION gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 184 Bio inert Quaternary Pump User Manual
99. sers can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues The Instrument Utilities is a basic version of the LabAdvisor with limited functionality required for installation use and maintenance No advanced repair troubleshooting and monitoring functionality is included Bio inert Quaternary Pump User Manual 69 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 70 Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 7 Error Information What Are Error Messages 73 General Error Messages 74 Timeout 74 Shut Down 74 Remote Timeout 75 Synchronization Lost 76 Leak 76 Leak Sensor Open 77 Leak Sensor Short 77 Compensation Sensor Open 78 Compensation Sensor Short 78 Fan Failed 79 Open Cover 79 Module Error Messages 80 Restart Without Cover 80 Solvent Zero Counter 80 Pressure Above Upper Limit 81 Pressure Below Lower Limit 82 Pressure Signal Missing 82 Missing Pressure Reading 83 Pump Configuration 83 Valve Fuse 84 Inlet Valve Fuse 84 Valve Failed MCGV SSV 85 Motor Drive Power 86 Encoder Missing 87 Inlet Valve Missing 87 Temperature Out of Range 88 2 Agilent Technologies n
100. sibility compensation Recommended pH range Gradient formation Composition range Composition precision Communications Materials in flow path Active seal wash Dual piston in series pump with proprietary servo controlled variable stroke drive floating pistons and active inlet valve integrated 4 channel degassing unit 0 001 10 mL min in 0 001 mL min increments 0 2 10 mL min lt 0 07 RSD or lt 0 02 min SD whatever is greater based on retention time at constant room temperature 1 or 10 pL min whatever is greater Operating range up to 60 MPa 600 bar 8700 psi up to 5 mL min Operating range up to 20 MPa 200 bar 2950 psi up to 10 mL min lt 2 amplitude typically lt 1 3 at 1 mL min isopropanol at all pressures gt 1 MPa 10 bar User selectable based on mobile phase compressibility 1 13 short term 14 Low pressure quaternary mixing gradient capability using proprietary high speed proportioning valve Delay volume 600 900 uL dependent on back pressure 0 95 or 5 100 user selectable lt 0 2 RSD at 0 2 and 1 mL min Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN optional Titanium Gold Platin Iridium Sapphire PEEK PTFE Included 1 For solvent compatibility refer to section Solvent information for parts of the 1260 Infinity Bio in ert LC system in the manual Bio inert Quaternary Pump User Manual 09
101. skiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 186 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA Bio inert Quaternary Pump User Manual Appendix 13 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Bio inert Quaternary Pump User Manual 187 Sound Emission Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 188 Bio inert Quaternary Pump User Manual Agilent Technologies on Internet Agilent Technologies
102. solvent to fill the gap inbetween The first piston has a stroke volume in the range of 20 100 ul Bio inert Quaternary Pump User Manual 13 1 From solvent bottle Pump chamber 1 Introduction Overview of the Hydraulic Path degasser Piston 1 Ball screw drive Gear Motor with encoder 14 depending on the flow rate The microprocessor controls all flow rates in a range of 1 uL min 10 mL min The inlet of the first pumping unit is connected to the active inlet valve The outlet of the first piston chamber unit is connected through the outlet valve and the damping unit to the inlet of the second piston chamber unit The outlet of the purge valve assembly is then connected to the following chromatographic system Pump chamber 2 Purge valve gt To column Inlet valve To waste Piston 2 Figure 4 Principle of the Pump Bio inert Quaternary Pump User Manual Introduction 1 When turned on the pump runs through an initialization procedure to determine the upper dead position of the first piston The first piston moves slowly upwards into the mechanical stop of the pump chamber and from there it moves back for a defined distance The controller stores this piston position in memory After this initialization the pump starts operation with the set parameters The active inlet valve opens and the down moving piston draws solvent into the first pump chamber At the same time the second piston move
103. solvent tubing can be used it is necessary to prime the system Isopropanol IPA is recommended as priming solvent due to its miscibility with nearly all HLPC solvents and its excellent wetting properties Description 1 lsopropanol Connect all modules hydraulically as described in the respective module manuals Fill each solvent bottle with 100 mL isopropanol Switch the system on When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can bear health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used The purge tool of the LabAdvisor or Instrument Utilities can be used for automatically purging the pump If the pump is not able to aspirate the solvent from the bottles a syringe can be used to draw the solvent manually through tubing and degasser Bio inert Quaternary Pump User Manual 41 3 42 Installing the Pump When priming the vacuum degasser with a syringe the solvent is drawn through the degasser tubes very quickly The solvent at the degasser outlet will therefore not be fully degassed Pump for approximately 10 minutes at your desired flow rate before starting an analysis This will allow the vacuum degasser to properly degas the solvent
104. ssed during the test and therefore would appear as leaks Using a degasser is highly recommended Then the flow path is blocked by a blank nut at any position between the purge valve and the TCC outlet Bio inert Quaternary Pump User Manual 97 8 Test Functions and Calibration pressure flow leak rate time In the first phase of the test the pump delivers flow at a rate of 200 uL until a pressure of 50 bar below the defined maximum pressure is reached In the second phase the pump delivers a small flow which is increased stepwise If there is a leak in the system the pressure will drop initially as the low flow cannot compensate the leak flow As soon as the pump flow rate exceeds the leak flow rate the pressure will increase again and the test is stopped at about 20 bar below the maximum pressure The point in phase 2 where the lowest pressure is reached and stays constant for a short time corresponds to the leak rate that is provided as a test result A leak rate smaller than 3 uL min is good enough for operating the pump reliably 98 Bio inert Quaternary Pump User Manual Test Functions and Calibration 8 Running the Test Parts required CAUTION p n Description 01080 83202 Blank nut Damage to pressure sensitive parts Even columns that are suitable for high pressures are sensitive to pressure drops that occur during this test gt Do not include any pressure sensitive parts to the flow path and choose a
105. table for the installed tubing typically 3 5 mL min for 5 min Swap the bottle of isopropanol with a bottle of solvent for your application Repeat steps 1 to 7 for the other channel s of the pump Install the desired column set the required composition and flow rate for your application and equilibrate the system for approx 10 minutes prior to starting a run Bio inert Quaternary Pump User Manual Installing the Pump 3 Table 4 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of When switching between lsopropanol the system reverse phase and normal Miscible with almost all phase both times solvents After an installation To clean the system when using buffers After changing aqueous solvents After the installation of normal phase seals P N 0905 1420 Ethanol or methanol HPLC grade water HPLC grade water Hexane 5 isopropanol Alternative to isopropanol second choice if no isopropanol is available Best solvent to re dissolve buffer crystals Best solvent to re dissolve buffer crystals Good wetting properties Bio inert Quaternary Pump User Manual 45 3 46 Installing the Pump Priming the System Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 4 Using the Pump Hints for Successful Use of the Quaternary Pump 48 Prevent Blocking of Solvent Filters 49 Al
106. tation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only WARNING The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed 22 Bio inert Quaternary Pump User Manual CAUTION Site Requirements and Specifications 2 Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never opera
107. te your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Bio inert Quaternary Pump User Manual 23 2 Site Requirements and Specifications WARNING Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Bench Space The module dimensions and weight see Table 2 on page 25 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 3 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use
108. th isopropanol at a flow rate of 2 mL min 5 Close the purge valve and set the flow to a rate adequate to achieve a pressure of 350 bar Pump 15 min at this pressure to wear in the seals The pressure can be monitored using your instrument control software or tool 6 Turn OFF the pump slowly open the purge valve to release the pressure from the system disconnect the restriction capillary and reinstall the bottle with the solvent for your application 7 Rinse your system with the solvent used for your next application 8 Replace the purge valve frit see Exchanging the Purge Valve Frit or the Purge Valve on page 119 Bio inert Quaternary Pump User Manual 125 9 Maintenance Maintenance of the Pump Head When When maintaining seal wash option Tools required p n Description 8710 2392 Hexagonal key 4 0 mm 15 cm long T handle Parts required p n Description 01018 23702 Insert tool 0905 1731 Wash Seal PTFE for Bio inert Pump 600 bar 01018 07102 G5611 21503 5067 4695 Gasket Seal wash Piston Seal PTFE Bio inert Sapphire piston Preparations Switch off pump at the main power switch Remove the front cover Use a solvent shutoff valve or lift up solvent filters for avoiding leakages Remove the pump head see Removing the Pump Head Assembly on page 122 Remove the wash solvent tubings from the support ring inlet and outlet CAUTION Seal life time Wrongly inserting the backup ring will strongly reduce
109. that remove algae e Exchange solvents every two days or refilter e Ifthe application permits add 0 0001 0 001M sodium azide to the solvent e Place a layer of argon on top of your solvent e Avoid exposure of the solvent bottle to direct sunlight Never use the system without solvent filter installed Bio inert Quaternary Pump User Manual 49 4 Using the Pump Algae Growth in HPLC Systems 50 The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing Blocked solvent filters or deposits on inlet or outlet valves resulting in unstable flow composition or gradient problems or a complete failure of the pump Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure PTFE frits blockage leading to increased system pressure Column filters to plug giving high system pressure Flow cell windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the
110. the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions Bio inert Quaternary Pump User Manual 3 In This Guide 8 Test Functions and Calibration This chapter describes the tests for the module 9 Maintenance This chapter describes the maintenance of the module 10 Parts for Maintenance This chapter provides information on parts for maintenance 11 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules 12 Hardware Information This chapter describes the pump in more detail on hardware and electronics 13 Appendix This chapter provides addition information on safety legal and web 4 Bio inert Quaternary Pump User Manual Contents Contents Introduction 9 Introduction tothe Pump 10 Overview of the Hydraulic Path 11 Bio inert Materials 17 Early Maintenance Feedback 19 Instrument Layout 20 Site Requirements and Specifications 21 Site Requirements 22 Physical Specifications 25 Performance Specifications 26 Installing the Pump 27 Unpacking the Pump 28 Optimizing the Stack Configuration 30 Installing the Pump 33 Connecting Modules and Control Software 36 Flow Connections of the Pump 38 Priming the System 41 Using the Pump 47 Hints for Successful Use of the Quaternary Pump 48 Prevent Blocking of Solvent Filters 49 Algae Growth in HPLC Systems 50 Solvent
111. the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 17 Boot Resident Settings without on board LAN Mode Select SW1 sw2 SW3 SW4 SW5 SW6 SW7 sws No LAN TEST BOOT 1 1 0 0 1 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 18 Forced Cold Start Settings without on board LAN Mode Select SW1 sw2 SW3 SW4 SW5 SW6 SW7 sws No LAN TEST BOOT 1 1 0 0 1 0 0 1 Bio inert Quaternary Pump User Manual 179 12 Hardware Information Setting the 8 bit Configuration Switch without On board LAN 180 Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 13 Appendix General Safety Information 182 The Waste Electrical and Electronic Equipment Directive 185 Batteries Information 186 Radio Interference 187 Sound Emission 188 Agilent Technologies on Internet 189 This chapter provides addition in
112. tional solvent cabinet a vacuum degasser and a four channel gradient pump The four channel gradient pump comprises a high speed proportioning valve and a pump assembly It provides gradient generation by low pressure mixing A solvent cabinet provides enough space for four one liter bottles The pump is optimized for the use of buffer solutions by the active seal wash function which cleans pistons and seals Seal wash not shown Pump head Outlet valve Purge valve Active inlet valve MCGV Degasser Figure 1 Overview of the bio inert quaternary pump 10 Bio inert Quaternary Pump User Manual Introduction 1 Overview of the Hydraulic Path The bio inert quaternary pump is based on a two channel dual piston in series design which comprises all essential functions that a solvent delivery system has to fulfill Metering of solvent and delivery to the high pressure side are performed by one pump assembly which can generate pressure up to 600 bar Degassing of the solvents is done in a built in vacuum degasser Solvent compositions are generated on the low pressure side by a high speed proportioning valve MCGV The pump assembly includes a pump head with an active inlet valve and an outlet valve A damping unit is connected between the two piston chambers A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head The pump includes an active seal wash function whi
113. tream of sample introduction G5667A e Titanium gold PTFE PEEK zirconium oxide Downstream of sample introduction PEEK zirconium oxide Agilent 1260 Infinity Bio inert Manual Injector PEEK zirconium oxide G5628A Agilent 1260 Infinity Bio inert Analytical Fraction Collector PEEK zirconium oxide PTFE G5664A Bio inert Quaternary Pump User Manual 17 1 18 Introduction Table 1 Bio inert Materials Module Materials Bio inert Flow Cells Standard flow cell bio inert 10 mm 13 pl 120 bar 12 MPa for MWD DAD includes Capillary Kit Flow Cells BIO p n G5615 68755 p n G5615 60022 for Agilent 1260 Infinity Diode Array Detectors DAD G1315C D Max Light Cartridge Cell Bio inert 10 mm V o 1 0 pl p n G5615 60018 and Max Light Cartridge Cell Bio inert 60 mm V o 4 0 pl p n G5615 60017 for Agilent 1290 Infinity Diode Array Detectors DAD G4212A B Bio inert flow cell 8 pL 20 bar pH 1 12 includes Capillary Kit Flow Cells BIO p n G5615 68755 p n G5615 60005 for Agilent 1260 Infinity Fluorescence Detector FLD G1321B Bio inert heat exchanger G5616 60050 for 1290 Infinity Thermostatted Column Compartment G1316C Bio inert Valve heads Bio inert Connection capillaries PEEK zirconium oxide sapphire PTFE PEEK fused silica PEEK fused silica PTFE PEEK steel cladded G4235A G5631A G5639A PEEK ceramic Al 03 based Upstream of sample introduction Titani
114. ts 99 System Pressure Test failed 101 Leak Rate Test 102 Running the Test 104 Evaluating the Results 104 Potential Causes of Leak Rate Test Failure 105 This chapter describes the tests for the module gy Agilent Technologies 95 8 Test Functions and Calibration Introduction 96 All tests are described based on the Agilent Lab Advisor Software B 01 04 Other user interfaces may not provide all tests listed here For details on the use of the interface refer to the interface documentation Table 7 Interfaces and available test functions Interface Comment Available Function Agilent Instrument Utilities System Pressure Test Agilent Lab Advisor All tests are available System Pressure Test Leak Rate Test Agilent ChemStation No tests available Agilent Instant Pilot System Pressure Test Bio inert Quaternary Pump User Manual Test Functions and Calibration 8 System Pressure Test Introduction The System Pressure Test is used for checking the tightness of the LC system and identifying leaks between the pump and a position in the flow path following the pump blocked by a blank nut Test Principle A solvent can be chosen from available solvent channels and a maximum pressure can be defined at which the test will be run In contrast to older revisions of this test any solvent can be used Before the test the pump and system are flushed with solvent in order to remove air bubbles as air bubbles are compre
115. type The compressibility compensation is set to a default value and can be changed through the user interface Without a compressibility compensation the following will happen during a stroke of the first piston The pressure in the piston chamber increases and the volume in the chamber will be compressed depending on back pressure and solvent type The volume displaced into the system will be reduced by the compressed volume Bio inert Quaternary Pump User Manual 15 1 16 Introduction With a compressibility value set the processor calculates a compensation volume that depends on the back pressure of the system and the selected compressibility This compensation volume will be added to the normal stroke volume and compensates the previously described loss of volume during the delivery stroke of the first piston How Does Variable Stroke Volume Work Due to the compression of the pump chamber volume each piston stroke of the pump will generate a small pressure pulsation influencing the flow stability of the pump The amplitude of the pressure pulsation depends mainly on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes generate pressure pulsations of smaller amplitude than higher stroke volumes at the same flow rate In addition the frequency of the pressure pulsations is higher This decreases the influence of flow pulsations on quantitative results In gradient mode smaller stroke
116. um Downstream of sample introduction Agilent uses stainless steel cladded PEEK capillaries which keep the flow path free of steel and provide pressure stability to more than 600 bar For ensuring optimum bio compatibility of your Agilent 1260 Infinity Bio inert LC system do not include non inert standard modules or parts to the flow path Do not use any parts that are not labeled as Agilent Bio inert For solvent compatibility of these materials see Solvent information for parts of the 1260 Infinity Bio inert LC system on page 51 Bio inert Quaternary Pump User Manual Introduction 1 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EM
117. ure which has been set for the test Then piston 1 is brought to its rear position An increasing flow is delivered by piston 1 In case of a leak the pressure will drop initially as long as the flow rate delivered by the piston is lower than the leak rate As soon as the flow rate of the piston exceeds the leak rate the measured pressure will increase again Therefore the minimum pressure of that curve segment corresponds to the flow and leak rate at that time and the leak rate is measured Compare to the description of the system pressure test System Pressure Test on page 97 Subsequently piston 2 is moved to its rear position then piston 2 delivers and the measurement is done as described for piston 1 Bio inert Quaternary Pump User Manual 103 8 Test Functions and Calibration Leak Rate Test Running the Test Parts required p n Description 01080 83202 Blank nut Running the test from the Agilent Lab Advisor 1 Select the Leak Rate Test from the Test Selection menu 2 Start the test and follow the instructions Make sure to release the pressure by slowly opening the purge valve when the test has NOTE finished Evaluating the Results Results of the leak rate test are the leak rates measured for pistons 1 and 2 as described for the test principle If any of the leak rates exceeds 3 uL min the test will fail Damper LED iM Pump chamber 1 Pump chamber 2 Purge valve Outlet valve gt To column Inlet
118. use bio inert parts only Item p n Description 1 G5611 60061 Purge Valve Bio inert 2 01018 22707 PTFE frits pack of 5 3 5067 4728 Seal cap 3 Figure 13 Purge valve assembly bio inert Bio inert Quaternary Pump User Manual 141 10 Parts for Maintenance Active Inlet Valve yf For bio inert modules use bio inert parts only p n Description G5611 60025 Active Inlet Valve AIV Bio inert without cartridge G5611 60020 Cartridge for AIV Bio inert 0100 1847 Adapter AIV to solvent inlet tubes 142 Bio inert Quaternary Pump User Manual Figure 14 Active inlet valve Bio inert Quaternary Pump User Manual Parts for Maintenance 10 Active Inlet Valve 143 10 Parts for Maintenance Accessory Kit Bio inert G5611 68755 144 Accessory Kit Bio inert p n G5611 68755 p n 5062 2461 5063 6527 5181 1519 G5611 60502 5042 9954 G5611 60500 Description Waste tube 5 m reorder pack Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste CAN cable Agilent module to module 1 m Capillary 900 x 0 17 mm titanium Bio inert pump to thermostatted autosampler Tubing clip 2x re order 4 pk Capillary 400 x 0 17 mm titanium Bio inert pump to injector Bio inert Quaternary Pump User Manual Parts for Maintenance 10 Starter Kit Bio inert Starter Kit Bio inert HPLC p n G5611 68707 p n Description 9301 1420 3x Solvent bottle transparent 9301 1450 Solvent bottle amber 01018 22707
119. w blinking modules without on board LAN indicator indicates that the module is in boot loader mode e g during update of main firmware In such a case try to re boot the module or try a cold start Bio inert Quaternary Pump User Manual 67 6 Troubleshooting and Diagnostics User Interfaces 68 Depending on the user interface the available tests vary Some descriptions are only available in the service manual Table 6 Test functions available vs user interface Test Instant Pilot G4208A Agilent Lab Advisor System Pressure Test Yes B 02 11 Yes B 01 04 Leak Rate Test No Yes B 01 04 SP1 Bio inert Quaternary Pump User Manual Troubleshooting and Diagnostics 6 Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition u
120. with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m Bio inert Quaternary Pump User Manual 165 11 Identifying Cables Agilent 1200 Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control Module 166 Bio inert Quaternary Pump User Manual Bio inert Quaternary Pump User Manual 12 Hardware Information Electrical Connections 168 Rear View of the Module 169 Interfaces 170 Overview Interfaces 172 Setting the 8 bit Configuration Switch without On board LAN 176 Communication Settings for RS 232C 177 Special Settings 179 This chapter describes the pump in more detail on hardware and electronics phe Agilent Technologies 167 12 Hardware Information Electrical Connections 168 The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropri
121. y Item p n G5611 60165 5067 4695 G1312 60062 G5611 63010 gt wo N 0905 1731 5065 9978 5 01018 07102 6 G5611 26210 7 G4220 24013 8 G5611 21503 9 G5611 25260 10 G5611 60025 G5611 60020 11 G5611 60067 12 5042 1303 13 G5611 60061 14 0515 2118 15 G1311 60161 Description Pump Head with Seal Wash Option Bio inert Sapphire piston Piston housing incl spring Support Ring with Seal Wash Option Bio inert Wash Seal PTFE for Bio inert Pump 600 bar Silicone tubing 1 mm i d 3 mm o d 5 m re order number Gasket Seal wash Seal Holder Bio inert Backup Seal for Support Ring Piston Seal PTFE Bio inert Pump Chamber Housing Bio inert Active Inlet Valve AIV Bio inert without cartridge Cartridge for AIV Bio inert Outlet Valve Bio inert Lock screw Purge Valve Bio inert Pump head screw M5 60 mm 1220 1260 Seal Wash Pump Assembly Bio inert Quaternary Pump User Manual Parts for Maintenance Pump Head Assembly with Seal Wash Option 10 Figure 11 Pump Head with Seal Wash Option Bio inert Quaternary Pump User Manual 139 10 Parts for Maintenance Outlet Valve Outlet Valve FM For bio inert modules use bio inert parts only Linert p n Description G5611 60067 Outlet Valve Bio inert Figure 12 Outlet valve bio inert 140 Bio inert Quaternary Pump User Manual Parts for Maintenance 10 Purge Valve Assembly Purge Valve Assembly FM For bio inert modules
122. y connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as Bio inert Quaternary Pump User Manual Hardware Information 12 e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 input load is 2 2 kOhm against 5 0 V and output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Table 11 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration dete

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