"TRAINING" - 8 APPLICATIONS - user`s manual
Contents
1. BLUESTAR FORENSIC Latent bloodstain reagent BLUESTAR FORENSIC TRAINING Ref BL 508 TR DESTROYS DNA USE FOR TRAINING PURPOSE ONLY The BLUESTAR FORENSIC is a patented powerful new la tent bloodstain reagent that has been designed to reveal blood either fresh dried or still humid washed away blood pure or diluted in large or minute quantity The use of chemicals to investigate crime scenes is well known and latent blood reagents such as Amido Black Benzidine Leucomalachite Leuco Crystal Violet Fluorescein Luminol Phenolphthalein Herma Glow etc have been ap plied for many years by forensic experts around the world to detect blood in minute quantity blood cells still present in washed away bloodstains or blood otherwise invisible to the human eye Two main categories of such chemicals exist for this purpose Those which react to the proteins and amino acids contai ned in the biological fluid DFO Amido Black etc Those which detect an enzymatic activity Benzidine teucomatachite green Ceucocrysrat viotet cumin Fluorescein ete However all these existing chemicals suffer from some major drawbacks Some are simply too toxic or too highly flammable to be used safely Others severely compromise or prevent DNA typing are not sensitive enough and are difficult to use Still others are highly unstable have a very short life are difficult to maintain in storage or2. Active life Best results are obtained when the product is used within 3 hours after mixing the tablets in water There is therefore no requirement to rush the investigation due to immediate product deterioration Covered area A 125 ml 4 fl oz dose is generally sufficient to investigate a 25 m 250 sq ft area to search a vehicle or an object clothes rug knife etc A liter 32 fl oz dose is generally sufficient to investigate about a 200 m 2 000 sq ft area Mixing procedure Open the spray bottle add 125 ml 4 fl oz of distilled water 2 Take a white tablet out of the white top tube and close the tube immediately Take a beige tablet out of the o top tube and close the tube immediately DO NOT switch caps The white cap goes on the white top tube and the orange cap on the orange top tube 3 Add the pair of tablets to the distilled water If you need more working solution use 125 ml 4 fl oz per pair of tablets 4 Twist the head with its plunger on the spray bottle firmly 5 Allow about or 2 minutes for complete dissolution and mixing of the chemicals swirling gently with a circular motion of your hand Do NOT shake the container upside down Lighting conditions The BLUESTAR FORENSIC latent bloodstain reagent pro duces a very bright and long lasting blue chemiluminescence that does not require total obscurity to be visible However at high blood dilutions the investigation will be much eas
3. any combination of the above To solve these problems Professor Loic Blum Ph D a re cognized authority in chemiluminescence and the research team of the Enzymatic and Biomolecular Engineering Laboratory of the French National Center for Scientific Research CNRS at the University Claude Bernard of Lyon UCBL France developed the new latent bloodstain reagent BLUESTAR FORENSIC When placed in contact with the iron contained in the heme nucleus of blood hemoglobin the BLUESTAR FORENSIC zes enzymatic peroxydase activity and emits an in tense blue 430 nanometer chemiluminescence visible in the dark The BLUESTAR FORENSIC can also produce a chemilumi nescence when reacting to some household detergents such as bleach or copper but differences in intensity emission spectrum and reaction time allow for visual differentiation by experienced users The BLUESTAR FORENSIC latent bloodstain reagent is based on a new proprietary patented formula that is totally DNA typing compatible extremely sensitive stable easy to use and produces a very bright and long lasting chemilumi nescence However the specific BLUESTAR FORENSIC TRAINING formula does DESTROY DNA and is there fore intended only for training purposes Do NOT use BLUESTAR FORENSIC TRAINING for actual investiga tions The BLUESTAR FORENSIC latent bloodstain reagent is extremely sensitive Blood is detected on any background material animal or
4. ier and the risks of missing a clue much lower if the product is applied in total darkness Indoors Close all the windows block all outside light sour ces and turn off all the lights Outdoors Wait for night time and turn off all area lights inan urban environment If necessary screen off distant light sources or even a very bright moon and work facing away from parasite lights Wait for at least minute to allow your eyes to adjust to darkness Once your pupils have dilated you will be able to better observe the BLUESTAR FORENSIC reaction Vaporization In order to prevent biological contaminations of the revea led traces wear personal protective equipment safety gog gles gloves dust respirator protective clothing Do not spray toward another person The BLUESTAR FORENSIC latent bloodstain reagent is designed to be vaporized from waist height in a fine mist Adjust the spray nozzle to obtain the finest mist possible Nery tittte of i it fs actually needed Over spraying does NOT result in improved blood detection and in case the DNA is only available in very low quantities over spraying might dilute it too much for collecting exploitable samples thus compromising its analysis Check how the product reacts by spraying BLUESTAR FORENSIC on a testing sample This test will also help you become familiar with the reaction on blood Spray lightly horizontally ahead of you at least 50 em 2 away from
5. ls of the scene as well 7 Re spray the blood print to reactivate a bright chemilu minescence reaction 8 Shoot several pictures using different shutter speeds ty pically 30 seconds Biological prints revealed with BLUESTAR FORENSIC TRAINING do not allow for subsequent DNA analysis For other purposes samples of the revealed biological prints are collected using the same methods as for any kind of bio logical prints 5 STORAGE CLEANING amp DISPOSAL Storage The BLUESTAR FORENSIC TRAINING latent blood stain reagent has a 3 year shelf life AFTER MANUFACTU RING expiration date imprinted on each cardboard box Be certain to close the tubes immediately with their respec tive caps after each opening If you wish to use the product after the expiration date we recommend that you perform a test to check the product effectiveness Note The product is warranted for 2 years after the DATE OF PURCHASE DO NOT attempt to store the product AFTER MIXING the tablets with water The mixed product is an active chemical compound that oxidizes Inert gases are constantly released and will in time accumulate under pressure in a sealed container causing swelling and leaking Cleaning Since the BLUESTAR FORENSIC latent bloodstain reagent is designed to be used on presumed blood on crime scenes all precautions and regulations related to the biohazards of blood apply when cleaning Disposal Dispose of unused mix in a
6. sink under running water Dispose of cleaning residues according to local state and federal regulations applying to the biohazards of blood MATE WIAR SAFETY BT HH af E Material safety data sheets MSDS for the BLUESTAR FORENSIC TRAINING are available in POF format on our web site www bluestar forensic com gb download php BLUESTAR l6 Avenue de la Costa PO box 246 Monte Carlo 98000 MONACO Tel 377 97 97 31 77 Fax 377 97 97 31 61 E mail i fo pbluestar forensic com www bluestar forensic com Update 08 30 2006 BLUESTAR 2006
7. the target in a side to side sweeping motion NOT pointing toward the ground Indoors Be attentive not to saturate walls and vertical surfaces in order not to create drippings as if you were Outdoors Py arent jon i Pay attention to wind direction if any Do not spray into the wind but use it to carry a light cloud of pro duct over the area being searched identifying false reactions When reacting to blood the BLUESTAR FORENSIC latent bloodstain reagent emits an intense light blue chemilumines cence in the 420 to 440 nanometer range However false reactions may occur due to the presence of certain house hold detergents chlorine some paints and varnishes cop per certain iron metabolizing plants such as lichens thyme and some tree mosses and certain soils containing iron Such false reactions are easily identifiable by the trained technician because their color brightness and duration dif fer from those of the typical reaction on blood Typically false reactions are markedly dimmer and whiter False reactions due to chlorinated detergents are often in teresting because they may reveal attempts to wash or clean bloodstains and to conceal a homicide 3 PHOTOGRAPHY Photography of latent blood prints developed with chemi luminescence is not fundamentally different from regular day The same four basic elements of photo _ aby ect hang sensitivity aperture opening and interact in exactl
8. vegetable in liquid or solid form Blood may be detected either pure or diluted while fresh or long after it has been deposited dried or even deteriorated Furthermore it allows for viewing the blood traces even if they have been rinsed away or cleaned away even on minute blood amounts The BLUESTAR FORENSIC latent bloodstain reagent pro duces such an exceptionally bright chemiluminescence that complete darkness is not required for use and no special photographic equipment is necessary to record the evidence Standard 35 mm cameras can be used with perfect results The BLUESTAR FORENSIC latent bloodstain reagent is prepared within minutes It is then applied lightly in a mist with a hand held fine spray bottle The blue chemiluminescence produced by the BLUESTAR FORENSIC latent bloodstain reagent is stil visible several minutes after the bloodstains have been treated To facilitate crime scene investigation BLUESTAR FORENSIC can be applied numerous times over the same bloodstains Manual MIXING INSTRUCTIONS Box content Each BLUESTAR FORENSIC TRAINING box holds 2 tubes containing 8 pairs of tablets to make 8 x 125 ml 8 x 4 fl oz of BLUESTAR FORENSIC chemiluminescent solution or liter 32 fl oz if all pairs are used together Required items Prior to mixing the product you will need the following items distilled water spray bottle mister equipped with an adjustable spray noze a
9. y the same way mannen of BLUESTAR FORENSIC detected blood prints is easy and produces excellent results The chemiluminescence produced by the reaction of the BLUESTAR FORENSIC latent bloodstain reagent to blood is bright enough that no special equipment is needed However relatively long exposures may be needed for maxi mum picture quality and a tripod and flexible cable release are highly recommended This will ensure the camera is mo tionless during the time of the exposure A 24 mm lens is recommended Film No special film is required but it is advisable to use low light fast films in order to obtain acceptably short exposure times A film speed of ASA 400 is generally suitable Lighting conditions Total darkness is not required Natural low intensity diffu sed light is preferred Artificial light tungsten or fluorescent produces yellowish or greenish pictures A direct flash should be avoided Instructions to obtain good pictures Set the camera on a tripod perpendicular to the area being photographed 2 Disable the automatic flash and the autofocus mode if the camera has one 3 Use a large lens aperture typically a f 2 8 stop value 4 Set the exposure time to B 5 Focus the lens manually over a spot of light provided by a flashlight over the blood area 6 Turn off all lights Darkness should not be complete Pictures shot in dimmed light will allow you to view not only the trace but other detai
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