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Leica MZ10 F User Manual

1. Assembling the Focusing Column with a Transmitted Light Base 16 An Overview of the Leica MZ10 F 44 Assembling the Focusing Column with an Incident Light Base 17 The Correct Interpupillary Distance 45 Motorized Focus Restricting the Travel Path 18 Using the Eyepieces 46 Optics carrier 19 Focusing 47 Iris Diaphragm 20 Adjusting the Resistance of the Focus Drive 48 Tube 21 Changing Magnification Zoom 49 Eyepieces 22 Double Iris Diaphragm 50 Objective 23 Ratchet Steps and Magnification Levels 51 UV Protection Screen Assembly 24 Parfocality More Comfort and Convenience for Your Work 52 Transmitted Light Base TL ST 25 Transmitted Light Base TL BFDF Before First Use 26 Transmitted Light Base TL BFDF 27 Magnification Factors of the Eyepieces 54 TL RC TL RCI 28 Health Notes 55 IsoPro Manual Mechanical Stage Assembly 29 Dioptric Correction 56 Leica IsoPro Motorized Mechanical Stage Assembly 31 Dioptric Correction and Parfocality 57 Leica EL6000 Safety Notes 35 Graticules 58 Leica EL6000 Preparations 38 Leica EL6000 Connection to the Fluorescence Lamp Housing 41 Photography amp Video 60 Photo Tubes and C mounts 61 Trinocular Video Phototube 50 62 Trinocular Video Phototube 100 63 Leica MZ10 F User Manual O Optics Accessories The Different Types of Objectives Bases Leica TL ST Transmitted Light Base Controls Leica TL ST Transmitted Light Base
2. Put a dust cover over the microscope when it will not be used for a long time Use dust caps to protect tube openings tubes without eyepieces and eyepieces Keep accessories in a dust free place when not in use Leica MZ10 F Cleaning polymer components Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsu itable cleaning agents and techniques can damage polymers User Manual Permitted measures gt Clean the microscope or parts of it using warm soapy water then wipe using distilled water For stubborn dirt you can also use ethanol industrial alcohol or isopropanol When doing so follow the corresponding safety regulations Remove dust with a pneumatic rubber bulb or with a soft brush Clean objectives and eyepieces with special optic cleaning cloths and with pure alcohol 108
3. Wickmann Series 181 www wickmann com Schurter Series SPT 5X20 www schurter ch Repairs Opening or working on the Leica EL6000 compact light source in any manner other than described above changing lamps changing fuses may be carried out by the supplier of the instrument or the supplier s authorized repre sentatives 87 Leica EL6000 Replacing the Lamp The procedure for replacing the lamp does not differ from the installation procedure outlined on Page 38 except for the fact that the exis ting lamp has to be removed first Replacing the lamp The lamp used in the instrument beco mes very hot during operation and has high internal pressure when hot Before chan ging the lamp it is mandatory to allow the inst rument to cool off for at least 20 minutes 1 Pull the power plug out of the socket so that the EL6000 is disconnected from the power system 2 Unscrew both screws of the cover using a 3 mm Allen key Leica MZ10 F 3 Remove the housing cover 4 Carefully lay the Leica EL6000 on its side so that the opening faces you This makes it easier to replace the lamp 5 Pull the pressure bolt back towards the front plate using the lever 6 Pull out the lamp and disconnect the plug from the coupling in the instrument 7 Install the new lamp as outlined on Page 38 beginning with item 6 User Manual 88 Fluorescence Filter Changer The patented FLUOIII filter syst
4. Important Safety Notes User Manual The individual modules of the Leica M stereo microscopy series include an interactive CD ROM with all relevant user manuals in 20 other languages Keep it in a safe place and readily accessible to the user User manuals and updates are also available for you to download and print from our web site www leica micro systems com This User Manual describes the special func tions of the individual modules of the Leica MZ10 F fluorescence stereomicroscope and the Leica fluorescence module for the Leica M stereomicroscopy series and contains impor tant instructions for their operational safety maintenance and accessories The Safety concept booklet contains additional safety information regarding the service work requirements and the handling of the stereomi croscope accessories and electrical accessories as well as general safety instructions Leica MZ10 F You can combine individual system articles with articles from external suppliers e g cold light sources etc Please read the user manual and the safety requirements of the supplier Before installing operating or using the instru ments read the user manuals listed above In particular please observe all safety instructions To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals User Manual Special manuals Specia
5. Electrical safety For indoor use only Supply voltage 100 240 V 10 Frequency 50 60 Hz Power consumption Max 210 VA Fuses 5x20 2 5 A slow blowing breaking capacity H Ambient temperature 0 40 C Relative humidity 10 90 Overvoltage category Il Pollution degree 2 Leica MZ10 F User Manual 37 Leica EL6000 Preparations Setting up the instrument Set up the Leica EL6000 so that the front is readily accessible and visible The ventilation slots on the sides and back panel of the device must not be covered gt Maintain a free room of at least 150 mm on the rear panel of the instrument Instructions for lamp replacement The lamp contains mercury Be absolutely certain to follow the handling instructions and safety notes provided with the lamp Maintain cleanliness when inserting the lamp Remove any clinging packaging materials The lamp used in the instrument beco mes very hot during operation and has high internal pressure when hot Before chan Leica MZ10 F ging the lamp it is mandatory to allow the inst rument to cool off for at least 20 minutes The Leica EL6000 compact light source A comes with a factory supplied lamp but the lamp is not installed when shipped This minimizes the danger of damage to the lamp during transport Before transporting the Leica EL6000 compact light source you first have to remove the lamp User Manual Inserting the la
6. Leica MZ10 F User Manual MICROSYSTEMS General Notes Safety concept Use in clean rooms Servicing Before using your microscope for the first time The Leica M series can be used in clean rooms gt Repairs may only be carried out by Leica please read the Safety concept brochure without any problems included with your instrument It contains additional information about handling and Cleaning care Do not use any unsuitable cleaning agents chemicals or techniques for cleaning Microsystems trained service technicians Only original Leica Microsystems spare parts may be used Bezpe nostn koncepce Responsibilities of person in charge of Sicherheitskonzept 5 Ohutusesk Never use chemicals to clean colored instrument afety conce Concent de securits surfaces or accessories with rubberized gt Ensure that the Leica stereomicroscope Concetto di sicurezza i i f i parts This could damage the surfaces and is operated maintained and repaired by Biztons gi eldirasok p Velho daino specimens could be contaminated by abra authorized and trained personnel only oncepcja bezpieczenstwa A Conceito de seguran a ded particles Bezpe nostn pokyny Vamos koncept Turvallisuus S kerhetskoncept feica ESME ga re gt In most cases we can provide special solu tions on request Some products can be modified and we can offer other accesso ries for use in clean rooms Leica MZ10 F User Manual
7. Open shutter or rotate intensity switch towards the right Check light guide Light guide damaged defective Repair or replace If these actions do not fix the problem send the Leica EL6000 compact light source to Leica service with an exact description of the malfunction User Manual 86 Leica MZ10 F Leica EL6000 Cleaning and Maintenance Before carrying out cleaning tasks switch off the Leica EL6000 compact light source Use only a dry lint free cloth for cleaning When cleaning ensure that no dust or other foreign bodies enter the device through the ventilation slots In case of damage even external damage immediately stop using the Leica EL6000 compact light source and send it to service If it will not be used for a long time protect the Leica EL6000 compact light source from dust using a suitable cover such as the plastic bag from the original packaging Leica MZ10 F Spare parts and accessories The only parts of the Leica EL6000 compact light source that may be replaced by the user are the instrument fuses and the lamp gt The replacement of the lamp is described on Page 88 Replacing the instrument fuse 1 Disconnect the Leica EL6000 from the power supply 2 Openthe drawer with the instrument fuses located close to the power supply input 3 Replace the instrument fuse User Manual Permitted replacement fuses Fuse 5x20 2 5 A slow blowing breaking capacity H
8. reinsert the lamp holder User Manual Precautionary measures When inserting the lamp ensure that the cables are inside the two metal clamps This prevents the cables from getting caught during insertion Leica TL BFDF Transmitted Light Base Controls Extension plate of the Transmitted Light Base TL Connector for cold light sources BFDF fiber optic light guide active f 10 mm end tube f 13 mm 1 Adapter plate for easy assembly of focu sing drives Standard stage 10 447 269 3 Buttonto toggle between bright field and dark field Button to toggle between bright field and dark field Leica MZ10 F User Manual 70 Leica TL BFDF Transmitted Light Base Operation Light intensity control Please observe the user manual in parti cular all safety regulations from the manufacturers of the light guide and cold light source gt Switch on the cold light source according to the manufacturer s user manual and adjust the brightness Transmitted light control The Leica TL BFDF transmitted light base has a control that switches the light from bright field to dark field Leica MZ10 F Bright field Bright field is suitable for examining translu cent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background Turn the control as far as it will go towards BF bright field Dark field In dark fi
9. Operation Leica TL ST Transmitted Light Base Changing bulbs Leica TL BFDF Transmitted Light Base Controls Leica TL BFDF Transmitted Light Base Operation Leica TL RC TL RCI Controls Leica TL RCI The Path folding Mirror Leica TL RCI Color Intensity and Temperature Leica TL RC TL RCI Operation Leica TL RCI Methods in Transmitted Light Leica TL RCI Relief Images Using Filters Leica IsoPro Non motorized Controls Leica IsoPro Motorized Controls System Illumination Leica EL6000 Leica EL6000 About the Instrument Leica EL6000 Operation Leica EL6000 Troubleshooting Leica EL6000 Cleaning and Maintenance Leica EL6000 Replacing the Lamp Leica MZ10 F Fluorescence 65 Filter Changer 90 About Fluorescence Microscopy 91 Simple Filter Holders 92 67 Equipping the Filter Changer 93 68 Observation without Fluorescence 94 69 Commissioning the Fluorescence System 95 70 71 72 MATS TL 97 73 74 Specifications 75 Leica MZ10 F Specifications 99 76 Leica EL6000 Specifications 100 17 Leica TL ST Transmitted Light Base 102 79 Leica TL BFDF Transmitted Light Base 103 80 Leica TL RC TL RCIM 104 81 Appendix Calculating the Total Magnification Field of View Diameter 106 83 Care Maintenance Contact Persons 107 85 86 87 88 User Manual 10 Leica MZ10 F Congratulations Congratulations on obtaining your
10. included with the Leica MZ10 F These filter holders have two empty openings for the observation beam paths The third opening to the illumination beam path is closed gt Use this filter holder if you want to work briefly no more than 15 seconds without fluorescence illumination A So that the filter holder is not heated it must not block the mercury light for longer than 15 seconds Leica MZ10 F Safety Notes b If you want to block the mercury light for longer than that activate the UV shutter Use the filter ifyou want to change to trans mitted light or incident light observation without fluorescence Always place a simple filter holder into the empty filter changer positions in order to protect yourself from UV radiation and protect the system from dust User Manual If you are using fewer than four filter holders with fluorescence filters always insert the included simple filter holders with closed illu mination beam path into the available posi tions Otherwise there is a risk of danger to the eyes from direct UV radiation from the third beam path 92 Equipping the Filter Changer When inserting the filter sets avoid touching the filters in order to avoid finger prints Clean dirty filters immediately using a soft lint free cloth and pure alcohol Leica MZ10 F Removing the simple filter holder The Leica MZ10 F is equipped at the factory with simple filter holders as dummies T
11. knobs with both hands and turn them towards each other until the desired resistance is reached during focu sing Leica MZ10 F User Manual 48 Changing Magnification Zoom All M series microscopes have an integra Zooming ted zoom The name indicates the zoom 1 Look into the eyepieces range covered The MZ10 F covers a zoom range of 10 1 2 Focus on the specimen 3 Rotate the magnification changer until the A The rotary knob for the zoom can be desired magnification is configured used either left or right handed Leica MZ10 F User Manual 49 Double Iris Aperture The built in double iris diaphragm regulates the depth of field Note that The greater the depth of field the darker the image becomes gt In the same way the exposure time beco mes longer for photographs The resolution decreases Iris closed Iris open Leica MZ10 F User Manual 50 Ratchet Steps and Magnification Levels The zoom button can optionally be operated Enabling and disabling ratchet steps Magnifications and either with or without ratchet steps Conti 1 Push the front button backwards to disable fields of view nuous zoom is possible when the ratchet steps the ratchet steps The formula on Page 106 provides additional are disabled which many users find convenient information about the magnifications and field On the other hand when the ratchet steps are 2 Push the rear button forwards to enable the of view
12. new Leica MZ10 F stereomicroscope We are convinced it will fully meet your expectations as never before have we applied our decades of experience in the areas of optics mechanical engineering and ergonomics in such an uncompromising manner The Leica MZ10 F embodies all the qualities you associate with the name Leica Microsystems excellent objectives high quality engineering and reliability Furthermore the modular design ensures that the Leica MZ10 F adapts perfectly to your needs no matter which accessories you require for your tasks The entire imaging system including the zoom objective and ErgoTube is apochromatically correctedwithmuchtechnologicaleffort Contrast sharp ness richness in detail resolution image and color fidelity are optimum In addition the patented illumination beam path guarantees at every zoom level that light utilization is at a maximum and that fluorescence images are intensely luminous on a jet black background Leica MZ10 F Though the reliability and robustness of Leica stereomicroscopes is world renowned the Leica MZ10 F requires a certain degree of care and atten tion Therefore we recommend that you read this manual It contains all the information you need regarding operation safety and maintenance Simply observing a few guidelines will ensure that even after years of intensive use your stereomicroscope will continue to work as smoothly and reliably as on the very first day We wish yo
13. of parfocality is lost thus you have to manually refocus each time you change the zoom level To also use parfocality with dioptric correction refer to the instruc tions on Page 57 Dioptric Correction and Parfocality Leica stereomicroscopes are parfocally matched The prerequisite for this is the correct setting of the diopters and the parfocality The following adjustments have to be carried out only once by each user Preparations gt Move the lever of the video phototube to the observation position and open the diaphragm gt If you are using the microscope carrier AX set it to stereoscopic observation Leica MZ10 F Adjusting 1 Set the dioptric correction for both eyepie ces to 0 Select the lowest magnification and focus on a flat specimen Select the highest magnification and read just the sharpness Select the lowest magnification again but do not look into the eyepieces Rotate the eyepieces counterclockwise in the direction as far as they will go 5 diopter settings User Manual 7 Look into the eyepieces 8 Slowly rotate each eyepiece individually in the direction until each eye sees the object sharply imaged 9 Select the highest magnification and refo cus if necessary Now if you adjust the magnification from the lowest to the highest level the specimen is always brought into sharp focus If not repeat the process Graticules Use Leica grat
14. 9 Leica MZ10 F The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base without the stage mounted Disassembly is performed in reverse order of the following steps User Manual Standard stage L Take the glass plate from the rectangular gap in the standard stage Position the stage on the transmitted light base in such way that the four holes align over those in the base Attach the stage to the base with the four supplied hexagon head screws Insertthe glass plate back into the standard stage TL RC TL RCI The base is delivered with the adapter plate installed The selected stage Leica IsoPro Cross stage or Leica standard stage 10 447 269 and the focusing drive will have to be mounted later Ensure that the instruments are unpacked on a level adequately sized and nonskid underlay Stage assembly The Leica TL RC RCI transmitted light base can be equipped with three different Leica stages The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base without the stage mounted Disassembly is performed in reverse order of the following steps Leica MZ10 F Standard stage 1 Take the glass plate from the rectangular
15. Calculation example Field of view diameter in the specimen NFOV Moxzxqxr User Manual 106 Care Maintenance Contact Persons We hope you enjoy using your high perfor mance microscope Leica microscopes are renowned for their robustness and long service life Observing the following care and cleaning tips will ensure that even after years and deca des your Leica microscope will continue to work as well as it did on the very first day Warranty benefits The warranty covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Leica MZ10 F Contact address However if your instrument should no longer function properly contact your technician your Leica representative or Leica Microsystems Schweiz AG CH 9435 Heerbrugg E mail contact stereo service leica microsystems com User Manual Care gt Protect your microscope from moisture fumes and acids and from alkaline caustic and corrosive materials and keep chemicals away from the instruments Plugs optical systems and mechanical parts must not be disassembled or repla ced unless doing so is specifically permit ted and described in this manual Protect your microscope from oil and grease Do not grease guide surfaces or mechanical parts 107 Care Maintenance Contact Persons continued Protection from dirt Dust and dirt will affect the quality of your results
16. ad understood and observe this User Manual and particularly the safety instructions Safety Instructions continued Repairs service work gt Refer to Safety Concept booklet gt Only original Leica Microsystems spare parts may be used Before opening the instruments switch off the power and unplug the power cable Touching the live circuit can cause injury Transport Use the original packaging for shipping or transporting the individual modules of the Leica M stereomicroscopy series and the accessory components gt In order to prevent damage from vibrations disassemble all moving parts that accor ding to the user manual can be assembled and disassembled by the customer and pack them separately Leica MZ10 F Integration in third party products gt Refer to Safety Concept booklet Disposal gt Refer to Safety Concept booklet Legal regulations gt Refer to Safety Concept booklet EC Declaration of Conformity gt Refer to Safety Concept booklet Health risks Workplaces with stereomicroscopes faci litate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the duration of uninterrupted work asthenopia and musculoskeletal problems may occur For this reason appropriate measures for reduction of the workload must be taken User Manual gt Optimal arrangement of workplace work assignments and work f
17. ating the lamp supply is shut Switch on delay Approx 60 s after lighting the protection off After cooling off the lamp power supply in the lamp Leica EL6000 compact light source is automatically Interface switched on again Type Remote control input for inter Protection screen If no light guide is plugged into the light output of nal shutter the instrument the light output is automatically Design 9 pin D Sub socket closed off so that no light can escape Remote signal level Shutter closed 8 to 0 8V Shutter open 2 4 to 8V Input current Approx 1 mA Footswitch Shutter closed Connection between pin 2 and 4 Current Approx 1 2 mA Shutter frequency Max 10 Hz Leica MZ10 F User Manual 101 Leica TL ST Transmitted Light Base Light source Halogen lamp 12V 20W Quick illuminant change Yes Illuminated area 50mm Power supply Input voltage 100 240 V frequency 50 60 Hz energy consumption 30 W max ambient temperature 10 40 C Connections Power plug power switch Weight 7 4 kg Illumination types Bright field Yes Dark field Yes single sided Oblique light No Relief Contrast System RC No CCIC Constant Color Intensity Control No Internal shutter lamp control No Integrated filter holder Yes Coated optics for increasing the color Yes temperature Matching of high num aperture No Remote cont
18. ca MZ10 F Cross stage assembly 1 Place the mechanical stage onto the base Pull the upper part of the mechanical stage carefully towards the user fixing the lower part onto the transmitted light base Attach the mechanical stage evenly to the three threaded holes Now move the mechanical stage as far as it will go in the direction of the column Insert the glass plate into the mechanical stage User Manual Leica IsoPro Motorized Mechanical Stage Assembly Basics 1 Unpack the mechanical stage from the 2 Secure the motorized mechanical stage to The transmitted light bases ofthe LeicaTL series transport packaging and position it on the the base using three M4 screws TL BFDF TL RC TL RCI are supplied with an transmitted light base installed extension plate The selected stage Leica IsoPro Cross stage or standard stage 10 447 269 and the focusing drive will have to be mounted later The motorized cross stage is a sensitive preci sion instrument During installation avoid subjecting the stage to impact or severe vibra tions 1 Microscope base 3x M4 screws 2 Motorizedcross stage Leica MZ10 F User Manual 31 Leica IsoPro Motorized Mechanical Stage Assembly cont d 3 Remove the two M4 screws and 4 Remove the two M4 screws and the sleeve 5 Remove the four shock absorbing cartons the holder from the cross stage from the mechanical stage from the mechanical stage A After remo
19. can be used to close the illumination beam path This feature protects the mercury lamp which should not be switched on and off too frequently If for instance you need to inter rupt your work you do not have to switch off the high pressure mercury burner to preserve sensitive specimens from being burned out or bleached out Switching the high pressure mercury burner on and off frequently decreases its life and causes delays as the lamp can only be switched back on after it has cooled Instead you can close the UV shutter and reopen it when you return to your workplace User Manual Close the UV shutter when you do not want to view the specimen with fluorescence illumina tion but only with transmitted light Shutter open Shutter closed 94 Commissioning the Fluorescence System After switching on the high pressure mercury Using the protection screen burner you must adjust the arc lamp immedia For observation with fluorescence light tely see User Manual M2 216 1 Do not switch always position the protection screen on the supply unit until you feel confident using correctly to protect the observer the Leica stereomicroscope Follow all safety instructions when handling Using the Leica EL6000 mercury lamps and the lamp housing Be sure to observe the instructions for the Leica EL6000 starting on Page 82 1 Switch on the supply unit see User Manual M2 216 1 2 Wait 2 3 minutes 3 Adjust t
20. ccordance with EN 61010 2 101 2002 EN 61010 1 2001 IEC 1010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use The instruments and accessories described in this manual have been safety tested and checked for possible hazards The responsible Leica affiliate or the main plant in Wetzlar must be consulted whenever the device is altered modified or used in conjunc tion with non Leica components that are outside of the scope of this manual User Manual Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims If any safety defects or malfunctions of the Leica EL6000 are identified the instrument must be immediately disconnected from the power system and secured against additional use For repair the Leica EL6000 must be sent to the supplier or an authorized representative of the supplier Leica EL6000 Safety Notes continued The Leica EL6000 compact light source generates high energy light with invisible components There is a risk of being dazzled or blinded by light Never look directly into the light guide output of the instrument or into the output of the light guide connected to the inst rument Always ensure that the output of the connected light guide is securely connected to the system to be illuminated before switching on the compact light source Furthermore before switching on the compact light source d
21. d light bases TL RC and TL RCI can be equipped with up to three filters available as accessories simultaneously By customer request the filters are also available as one off items 1 Switch off the light source or click Leica TL RCI the button for the shutter Leica MZ10 F 2 Take the empty filter from an available filter slot in the filter holder 3 Insert the desired filter 4 Switch the light source back on User Manual Daylight filter for Leica TL ST A daylight filter is also available for the Leica TL ST transmitted light base 79 Leica IsoPro Non motorized Controls Operating the Leica IsoPro Cross stage 1 To move the stage in the X direction rotate the outer knob 2 To move the stage in the Y direction rotate the inner control ring Leica MZ10 F User Manual 80 Leica IsoPro Motorized Controls IsoPro X Y Stage DCI module Leica PSC Controller 1 Sub D interface for Leica SmartMove 1 Quick control memory function 2 3CTL2 interfaces 2 Fine control in X direction a 3 USB interface type B 3 Fine control in Y direction 1 Leica IsoPro Motorized Cross stage 4 Socket for grounded power cable 2 Housing with motorization Leica MZ10 F User Manual 81 System Illumination Leica EL6000 Leica EL6000 About the Instrument The Leica EL6000 compact light source is inten ded for biological routine and research work including the examination of sp
22. diameters with consideration given to enabled photographs measurement results ratchet steps the position of the magnification changer and etc can be reproduced more accurately the eyepiece and objective combination used Leica MZ10 F User Manual 51 Parfocality More Comfort and Convenience for Your Work All Leica stereomicroscopes are parfocally matched meaning that you can view a focused specimen from the lowest to the highest magnification without having to refocus The focus needs to be readjusted only if you want to view a specimen detail that is located higher or lower Requirements for parfocal work If you are using an eyepiece with dioptric correction the procedure differs from this description For more information refer to Page 57 Leica MZ10 F Parfocality 1 Enlarge the view to the maximum level 2 Focus on the specimen You are done Even if you select a smaller working distance the specimen remains pin sharp The parfocality is maintained until you focus on another level of the specimen User Manual Eyepieces Magnification Factors of the Eyepieces An eyepiece not only makes it possible to look passively into the micro scope but also has a critical effect on the maximum magnification The magnification factor is between 10x and 40x The following eyepieces are available for the M series Magnification Dioptric Correction Order number 10x 5 diopter se
23. e available light is divided as follows gt 50 is available for the two eyepieces gt 50 of the light is diverted to the video photo beam path Assembly Fasten the trinocular tube 50 to the optics carrier instead of the binocular observation tube Leica MZ10 F User Manual 62 Trinocular Video Phototube 100 Use Toggle With its third beam path the trinocular video Turn the controller on the right side of the phototube 100 enables you to either view or tube into the horizontal position in order to photograph a specimen This means that 100 guide all available light into the eyepieces of the light is available to one or the other beam You can now observe the specimen path The other beam path remains opaque or black gt Turn the controller on the right side of the tube into the vertical position in order to Assembly guide all available light into the camera Fasten the trinocular tube 100 to the optics You can now photograph the specimen carrier instead of the binocular observation tube Leica MZ10 F User Manual 63 Objectives and Optical Accessories The Different Types of Objectives To meet the various requirements regarding imaging properties there is a choice of high quality interchangeable planachromatic and planapochromatic objectives and also lower priced interchangeable achromatic objectives gt Achromatic objectives are particularly suited for specimens wit
24. e stereomicroscopes Intended use gt Refer to Safety Concept booklet Non intended use gt Refer to Safety Concept booklet Never use fluorescence stereomicroscopes or their components for surgical procedures e g on the eye unless they are specifically intended for that purpose The instruments and accessories described in this User Manual have been tested for safety and potential hazards The responsible Leica affiliate must be consulted whenever the inst rument is altered modified or used in conjunc tion with non Leica components that are outside of the scope of this manual Leica MZ10 F Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims Place of use gt Refer to Safety Concept booklet Electrical components must be placed at least 10 cm away from the wall and from flammable substances gt Avoid large temperature fluctuations direct sunlight and vibrations These condi tions can distort measurements and micro graphic images gt In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus User Manual Responsibilities of person in charge of instrument gt Refer to Safety Concept booklet Ensure that The M series stereomicroscopes and accessories are operated maintained and repaired by authorized and trained person nel only gt All operators have re
25. ecimens from the human body for the purpose of gaining information The Leica EL6000 compact light source is intended exclusively for operating mercury short arc lamps of type HXP R120W 45C VIS from OSRAM in conjunction with a suitable light guide for the instrument Any other use of the instrument shall be considered noncompli ant use Leica MZ10 F Special features The Leica EL6000 compact light source has an automatic beam cover This ensures that the light beam output of the instrument when the light guide is detached This prevents the user from being harmed by the intense light beam generated by the instrument The Leica EL6000 compact light source has a built in shutter It can be operated at the inst rument itself by means of a pushbutton or viaa remote control input The shutter is open when the open LED is illuminated The brightness can be varied in 5 increments 0 100 For this purpose a mechanical dimming unit that can be operated using a rotary switch on the front of the unit is installed in the device User Manual Heat absorbing filter When the cover is open the holder for the heat filter can be accessed Here hardened heat absorbing filters for protecting the light guide and various conversion filters with a diameter of 32 mm for adapting the spectrum to the application can be inserted The Leica EL6000 comes factory equipped with a heat absorbing filter 83 Leica EL6000 About
26. ed ambient temperature 0 to 40 C Permitted relative humidity Operational data 10 to 90 non condensing Power input Design Switched socket IEC320 C13 EN 60320 C13 Power supply voltage 100 to 240 VAC 10 Power frequency 50 to 60 Hz Application area Altitude for use Enclosed rooms max 2000 m above sea level Protective class Fuse protection 2x T2 5 A Breaking capacity H manufacturer Wickmann Type of protection IP20 EN 60529 EMC Emitted interference EN 61326 Class B Immunity to interference EN 61326 Appendix A Voltage fluctuations flicker EN 61000 3 3 Harmonic currents EN 61000 3 2 The instrument fulfills the requirements of EC Directive 89 336 EEC and the EMC law of September 18 1998 Series 181 Power consumption Max 210 VA Lamp Type Mercury short arc reflector lamp Designation OSRAM HXP R120W 45C VIS Power consumption 120 W Leica MZ10 F Service life manufacturer information User Manual Average value 2000 h 100 Leica EL6000 Specifications cont d Light output Protective functions Design Type Storz long Safety circuit If the cover is open all components in the instru Special feature Self locking if no light guide is ment except for the power input and power plug plugged in are disconnected from the power supply voltage Fan Overheating In the event of overhe
27. eica MZ10 F Tilted mirror The built in mirror features one flat and one concave side and can be rotated and moved The concave side has been specially designed for the optical requirements of objectives with a high numerical aperture The black rotary knob on the left side of the transmitted light base can be used to rotate the built in path folding mirror and move it forwards back The concave cutout on the handle indicates the concave side of the mirror allowing intuitive operation at any time without eye contact The angle of light incidence in the specimen plane changes depending on the tilt and position of the mirror As a result switching between transmitted light bright field oblique illumination and dark field like illumination is possible User Manual Functions of the rotary knob The rotary knob 1 fulfills the following tasks Turning the path folding mirror from the flat to the concave side Slightly tilting to guide the light beam through the specimen plane at a steeper or flatter angle Moving the path folding mirror forwards back 73 Potentiometer for controlling the CCIC Constant Color Intensity Control illumina tion intensity Potentiometer for controlling the color temperature Leica MZ10 F Leica TL RCI Color Intensity and Temperature The transmitted light base TL RCI has two electronic potentiometers that control the color intensity 1 and color temperature 2 T
28. eld illumination a ring illuminator is used in such a way that the direct light does not reach the objective without a speci men Only the structure of semitransparent opaque objects disperses the light making the object visible against a dark background Turn the control as far as it will go towards DF dark field Fingertip with bright field illumination User Manual Identical subject with dark field illumination Leica TL RC TL RCI Controls Vertical column transmitted light base adapter plate 1 Power switch l l IL 2 Power connection socket 1 reat sink of the eee halogen illumi 3 USBsocket type B Nation only TL RCI 4 USB socket type A Extension plate for focusing drives 5 2 CAN bus 6 Standard stage 10 447 269 Filter holder Control of top and bottom flaps of the Rottermann Contrast 6 Button for mirror and horizontal move ment of the mirror 7 Transmitted light base Screws for changing the halogen lamp Mm A WwW NN Leica MZ10 F User Manual 72 Leica TL RCI The Path folding Mirror Inverted world Depending on the properties of the specimen refractive index of the environment and the perception of the viewer it is possible that the switches described below for positive and inver ted relief contrast are to be operated in reverse This means that the lower switch rather than the upper switch controls the inverted relief contrast and vice versa L
29. em consists of a filter changer for barrier and excitation filters a UV shutter that can be activated deactivated and a filter insert for a filter holder with your choice of filter The filter changer can hold a total of 4 filter sets The filter sets for fluorescence are labeled The scope of delivery of the Leica MZ10 F inclu des four filter holders for empty filter positions and transmitted or incident light observation without fluorescence These filter holders have two empty openings for the observation beam paths The third opening to the illumination beam path is closed Leica MZ10 F Ifyou are using fewer than four filter holders with fluorescence filters always insert the included simple filter holders with closed illu mination beam path into the available posi tions Otherwise there is a risk of danger to the eyes from direct UV radiation from the third beam path A selection of the available filters including simple filter holder bottom right User Manual A The simple filter holders also protect the system from dust Filter sets for fluorescence The filter sets for fluorescence contain on one filter holder two barrier filters to the visual beam paths and one excitation filter to the illu mination beam path The filter sets are labeled 90 About Fluorescence Microscopy Functional principle Fluorescent substances light up when irradia ted with short wave excitation light and fluo resce
30. ept in the correct position at all times and permits smooth changeover between bright field and opaque transmitted light User Manual Bright field Bright field is suitable for examining translu cent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background Move the slider backwards until the desired effect is achieved Inclined transmitted light Transmitted light that traverses the object obli quely will provide additional resolution and information when observing semitransparent opaque objects gt Slowly pull the slider towards yourself until the desired effect is achieved Leica TL ST Transmitted Light Base Changing lamps Changing the halogen lamp Before you change the lamp it is absolutely necessary to unplug the power plug from the base to prevent the risk of electric shock The halogen lamp becomes very hot during operation Therefore to avoid being burned let the base cool off for approx 10 minutes after switching it off Do not touch new halogen lamps with your bare fingers this drastically reduces the service life of the lamp Leica MZ10 F Changing lamps 1 Unscrew the two screws on the heat sink and pull the heat sink out along with the lamp Carefully pull out the lamp and mount by pulling them upwards Disconnect the lamp from the mount Insert the new lamp into the mount and
31. gap in the standard stage Position the stage on the transmitted light base in such way that the four holes align over those in the base Attach the stage to the base with the four supplied hexagon head screws Insert the glass plate back into the standard stage User Manual IsoPro Manual Mechanical Stage Assembly Leica IsoPro Cross stage Before the Leica IsoPro Cross stage is moun ted to the base the axis containing the control buttons is attached either on the left or the right side of the cross stage Leica MZ10 F Left or right operation If the controls are to be mounted on the left hand side the gear rod on the bottom side of the cross stage must be unscrewed and reat tached in reverse H The install the operating elements skip over the first three items of the following description User Manual Assembly 1 Take the glass plate from the mechanical stage Turn the mechanical stage around and place it onto a non slip surface Change the gear rod from the left to the right hand side Leica lsoPro Manual Mechanical Stage Assembly cont d Control assembly La Take the glass plate from the mechanical stage and turn it around 2 Attach the axis with the control buttons to the desired side The fastener snaps into the cross stage magnetically 3 Attach the axis with the two supplied hexa gon head screws 4 Attach the cover rail to the mechanical stage Lei
32. h high contrast structures gt Flat field planachromatic objectives are particularly well suited for studying flat objects such as wafers and thin sections gt With planapochromatic objectives the finest structures are visible with high contrast The sophisticated apochromatic correction allows these objectives to attain the highest color brilliance and fidelity We recommend the planapochromatics of the M series article numbers 10 450 027 to 10 450 030 Leica MZ10 F User Manual 65 Leica MZ10 F User Manual 66 Leica TL ST Transmitted Light Base Controls Extension plate of the Transmitted Light Base TL ST Rear side of the transmitted light base TL ST 1 Screws for changing the halogen lamp 2 Power connection socket 1 Adapter plate for easy assembly of focu 3 Power switch sing drives Removable glass plate 3 Controller for light intensity Adjustment for path folding mirror Leica MZ10 F User Manual 67 Leica TL ST Transmitted Light Base Operation Light intensity control The left control adjusts the intensity of the 12V 20W halogen illumination 1 Switch on the illumination of the base at the power switch 2 Focuson the specimen 3 Set the illumination to the desired intensity using the left control Leica MZ10 F Transmitted light control The transmitted light base TL ST has a slider that automatically moves the path folding mirror in the base when moved The mirror is k
33. he arc lamp in accordance with User Manual M2 216 1 Leica MZ10 F User Manual 95 Dimensional Drawings MZ10F dimensioned drawings in mm a_i Leica MZ10 F User Manual 97 Specifications Leica MZ10 F Specifications Specifications Zoom ratio 10 1 Optics carrier magnification 0 8x 8x Design engineering CMO Common Main Objective optics Standard magnification 8x 80x 10x eyepiece amp 1 0x objective Standard objective Planapochromatic 1 0x NA 0 125 Illumination TripleBeam Third separate illumination beam path with automatic adjustment via zoom to the field of view Filter Changer Illumination control FLUOIII 4 positions manual Manual excitation shutter Diameter of the field of view Maximum resolution 131 1 31 mm max min 1 334 750 Ip mm Numerical Aperture Click stops for 0 25 max 1x 1 6K 2x 2 5x 3 2x 4x 5x 6 3x Leica MZ10 F User Manual 99 Leica EL6000 Specifications Dimensions WxHxD Electrical safety Widthxheightxdepth 130 mm x210 mm x 290 mm in accordance with EN 61010 1 2001 Environmental conditions Pollution degree Permitted ambient temperature 20 to 85 C Overvoltage category Permitted relative humidity 10 to 90 non condensing Operation Permitt
34. he controller for the color temperature simul taneously serves as an electronic shutter To interrupt your work click the potentio meter 2 Click again to switch on the illumination The electronics returns the color tempera ture to the previously configured setting User Manual Using a USB mouse only TL RCI The Leica USB mouse controls the CCIC and dimming function of the TL RCI base Connect the mouse to the corresponding USB port of the base The scroll wheel of the mouse is occupied by default with the CCIC control system and is used to control the illumination intensity To switch the illumination on or off briefly click the scroll wheel To enter or exit dimming mode click and hold down the scroll wheel for longer than 2 seconds 74 Leica TL RC TL RCI Operation Setting the relief contrast The two switches on the left side of the trans mitted light base TL RC TL RCI activate two built in flaps The outer switch 1 controls the inverted relief contrast and the inner switch 2 adjusts the positive relief contrast 1 Switch for adjusting the inverted relief contrast 2 Switch for adjusting the positive relief contrast 3 Path folding mirror Leica MZ10 F Depending on the flap position a part of the opening of the built in Fresnel lenses are covered which results in the different contrast effects Phase structures typically act as spatial relief type image
35. hese must be removed before inserting a filter 1 Grasp the recess of the dummy in the filter changer and pull it out User Manual Inserting the filter 1 Place the filter into the filter changer Ensure that the contour of the filter holder matches the contour of the microscope 2 Rotate the filter changer by hand until it latches into place If the filter holder has not been inserted correctly the filter changer does not rotate 3 Insert a total of four filters Slot for filter slide There is a slot in the filter changer system for an individually selectable filter for example a neutral density filter 93 Observation without Fluorescence We recommend that you first familiarize H yourself with the controls of your Leica fluorescence microscope in transmitted light without the presence of fluorescence illumina tion After switching on the high pressure mercury burner you must adjust the arc lamp immediately see User Manual M2 216 1 We recommend that you first familiarize H yourself with the basic functions of your Leica stereomicroscope as they are described in the previous section of this User Manual These include the binocular tube the eyepieces the zoom magnification changer the focusing drive and others The following describes the specific functions of the fluorescence system only Leica MZ10 F Observation without Fluorescence The FLUOIII filter system includes a UV shutter that
36. icrosystems offers a variety of adapters that can be used together with the 50 and 100 trinocular tubes Leica MZ10 F Leica DFC cameras However if you require absolute control over the camera and need the capability for measu rement evaluation and more in addition to photography the digital Leica DFC cameras are exactly right for you Together with the Leica Application Suite they provide virtually limit less freedom of use For additional informa tion about Leica cameras refer to the camera s documentation User Manual Leica Application Suite The Leica Application Suite or LAS for short is as it were the digital extension of the Leica M series microscopes In addition to capturing images it lets you control the microscope illu minator stages cameras and more For additi onal information refer to the LAS online help 60 Photo Tubes and C mounts Application All Leica DFC cameras are equipped with a standardized C mount interface In turn the C mount adapter for the respective trinocular tube is connected to this interface This adapter creates a solid mechanical connection between the microscope and camera and ensures opti mum rendering of the microscopic image on the image sensor of the camera Usually the ideal is for the digital camera to capture as much of the field of vision as possi ble while excluding as much of the black edge of the field of vision as possible To do so the mag
37. icules make length measurements and counting easier particularly for work stations that are not equipped with a digital camera and LAS software The Leica graticules for length measurements and numbering are fitted in mounts and are inserted into the eyepieces 1 Screw the insert off of the eyepiece Leica MZ10 F Clamp the graticule on the insert applying moderate pressure Ensure that the grati cule fits tightly Screw the insert and graticule firmly into place and replace the eyepiece in the tube User Manual 4 You can now align the graticule by rotating the eyepiece in the tube and then tighte ning it using the clamping screw Use with the Leica AX carrier Due to the convergence angle in the Li stereoscopic image measurements can provide only approximate values Photography amp Video Photography amp Video For most microscope users digital documen tation has become an invaluable part of their work Research results can be presented in an attractive manner measurements on the digi tal image provide clarity and in conjunction with the motorized IsoPro cross stage even images of large specimens can be captured step by step and automatically joined to create a new complete image Adapters If camera control using the Leica Application Suite is not required conventional mirror reflex and rangefinder cameras from third party manufacturers can be used For this purpose Leica M
38. im the light output all the way using the intensity switch left limit stop of the switch to prevent damage to the connected system To prevent damage to the light guide connected to the device the heat absor bing filter inserted into the device must be free of damage gaps cracks etc If the heat absor bing filter is not inserted or is defective the service life of the light guide will be decreased Leica MZ10 F The interior of the lamp inserted into the unit contains highly toxic substances At the end of its service life dispose of the lamp according to applicable regulations The manu facturer of the lamp provides corresponding information for example in the lamp s shipping documents Follow the manufacturer s instruc tions in case the lamp bursts The Leica EL6000 compact light source is designed for a voltage range of 100 240 VAC 50 60 Hz Within this voltage and frequency range the instrument always adapts to the connected power supply Opera ting the instrument with a power supply voltage outside this range can destroy the inst rument and the connected components User Manual Only fuses of the specified type and the listed rated current may be used as replace ments The use of incorrect fuses may result in a fire hazard The instrument is intended exclusively for operation in dry rooms Do not use the inst rument in rooms with explosion hazard Leica EL6000 Safety Notes continued
39. ingle circular image field when looking at a specimen If you are still a novice microscope user you may need a short time to become accustomed to this Not to worry after a little while it will become automatic Reference value The distance between eye and eyepi ece measures approx 22 mm for 10 23B wide field eyepieces for persons wearing glasses Leica MZ10 F Adjusting the interpupillary distance 1 Look into the eyepieces 2 Hold the eyepieces with both hands Push the eyepieces together or separate them until you see a circular image Slowly approach the eyepieces with your eyes until you can see the complete image field without corner cutting User Manual 45 Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer Simply push them into the tube and they are ready to use Each eyepiece offers a certain magnification factor that has a determinative effect on the total magnification Furthermore all Leica eyepieces can be equipped with practical grati cules that enable measuring and quantifying of specimens Dioptric Correction A built in dioptric correction is available for eyeglass wearers For more information refer to Page 56 Leica MZ10 F If you do not wear glasses 1 2 Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise If an eyepiece is equipped with the integ rated dioptric correction turn the va
40. l manuals are provided for a number of accessories User Manual M2 216 1 for lamp housing with high pressure mercury burner User Manual for ebq 100 isolated supply unit for 100W high pressure mercury burners User Manual M2 267 1 for the motorized focus system Symbols Used Warning Safety hazard This symbol indicates especially impor tant information that must be read and complied with Failure to comply can cause the following Hazards to personnel Functional disturbances or damaged inst ruments Warning of hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following Hazards to personnel Functional disturbances or damaged inst ruments Leica MZ10 F Danger due to hot surface This symbol warns against touching A accessible hot surfaces e g those of light bulbs Important information This symbol indicates additional infor mation or explanations that are intended to provide clarity Explanatory notes gt This symbol within the text stands for addi tional information and explanations Figures 1 Numbers in parentheses within the descriptions relate to the figures and the items within those figures User Manual Safety Instructions Description The individual modules fulfill the highest requi rements for observation and documentation with the Leica fluorescenc
41. ld dark field Yes yes Yes yes Oblique light Relief Contrast System RC Yes yes Yes yes CCIC Constant Color Intensity Control No Yes Internal shutter lamp control Yes Yes Integrated filter holder Yes Yes Coated optics for increasing the color temperature Yes Yes Matching of high num aperture Yes Yes Remote control options Yes Yes AntiShock Pads Yes Yes Dimensions of base WxHxD 340x390x95 mm 340x440x95 mm Single sided With cold light source Leica CLS150 LS Concave mirror With external light source Leica MZ10 F User Manual 104 Appendix Leica MZ10 F User Manual 105 Calculating the Total Magnification and Field of View Diameter NFOV Field number of the eyepiece Field numbers are printed on the eyepieces 10x 21 16x 14 25x 9 5 40x 6 Example MO 1x objective ME 25x 9 5 eyepiece Parameter MO Magnification of the objective ME Magnification of the eyepiece Z Magnification of the changer position q Tube factor e g 1 5x for coaxial incident light 1 6x for 45 ErgoTube r Factor 1 25x if the planachromatic and planapochromatic objectives of the MZ125 MZ16 are used on the MS5 MZ6 MZ75 or MZ95 Calculation example Magnification in the binocular tube MTOT VIS MO x MExzxqxr or 1x25x4x 1 5 x 1 25 187 5x Leica MZ10 F Z Zoom position 4 q Coaxial incident light 1 5x tube factor r Factor 1 25x
42. low changing tasks frequently Thorough training of the personnel giving consideration to ergonomic and organizati onal aspects The ergonomic optical design and cons truction of the Leica M stereomicroscopy series are intended to reduce the exertion of the user to a minimum Safety Instructions continued Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups Leica MZ10 F Light sources Safety instructions Protective measures of the manufacturer gt UV protection screen in front of the speci men plane prevents the user from looking directly into the UV rays Dummy filter carriers in the free positions of the rapid filter changer prevent direct UV radiation from reaching the eyes UV filters are installed in the observation beam paths to protect the eyes The stray light protection on the lamp housing prevents irradiation of the hands User Manual Warning UV radiation could damage the eyes There fore gt Never look into the light spot on the speci men plane without a UV protection screen gt Never look into the eyepieces if no excita tion filter is in the beam path Fill empty filter positions with dummy filter carriers Do not select a white strongly reflective background for the specimen Safety Instruction
43. lue to the 0 mark User Manual If you wear glasses 1 Hold the eyepiece firmly and rotate the eyecups clockwise towards the rear as otherwise the viewing distance is too great 2 Ifan eyepiece is equipped with the integ rated dioptric correction turn the value to the 0 mark By the way one beneft of viewing with eyeglasses is a drastically lower risk of bacterial transmission The soft material of the eyecup also ensures that your glasses will not be scrat ched even if they contact the eyepiece 46 Focusing Focusing raises or lowers the stereomicroscope Focusing Coarse fine adjustment using the focusing drive The specimen detail is gt The inner coarse adjustment is used for The sharpness is adjusted using the coarse fine brought into sharp focus as soon as it is in the covering great distances adjustment The resolution of the coarse fine focal point of the objective adjustment is 1 um The outer fine adjustment is used for fine gt The focusing drive can be operated either focusing The coarse fine adjustment carries a load of left or right handed up to 15 kg Leica MZ10 F User Manual 47 Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too loose or too tight Does the equipment tend to slide downwards The resistance can be adjusted individually depen ding on the equipment weight and personal preferences as follows 1 Grip the outer drive
44. mid posi motor into one of the three available CTL2 tion interfaces 4 Connect the PC where applicable and X Y 2 Plug the CTL2 plug of the Leica PSC control Stage DCI Module using a suitable USB ler into another CTL2 interface cable The third CTL2 interface is available for connecting other instruments such as the Leica foot switch 10 447 398 or TL RCIM transmitted light base 10 446 352 k The 15 pin Sub D interface is intended for use with the Leica SmartMove controller 11 501 197 3 Plug a power cable into the socket provided and into a grounded power socket As soon as all instruments have been plugged into the control box and it has been plugged Leica MZ10 F User Manual 34 Leica EL6000 Safety Notes The manufacturer assumes no responsi bility or liability for any use outside of the intended use or use outside of the specifica tions from Leica Microsystems Wetzlar GmbH or any risks resulting from such use In such cases the Declaration of Conformity shall be invalid This IVD device is not intended for use in the patient environment defined by DINVDE 0100 710 Itis also not intended to be combined with medical devices as defined by EN 60601 1 If a microscope is electrically connected to a medical instrument in accordance with EN 60601 1 the requirements defined in EN 60601 1 1 shall apply Leica MZ10 F General safety notes This safety class 1 device was built and tested in a
45. mp 1 Pull the power plug out of the socket so that the EL6000 is disconnected from the power system 2 Unscrew both screws of the cover using a 3 mm Allen key 3 Remove the housing cover Leica EL6000 Preparation continued 4 Carefully lay the Leica EL6000 on its side so 5 Pull the pressure bolt back towards the Ensure that the groove in the contact surface of that the opening faces you front plate using the lever the lamp matches up with the corresponding plug in the lamp mount This makes it easier to insert the lamp 6 Insertthe lamp Leica MZ10 F User Manual 39 Leica EL6000 Preparation continued 7 When the lamp makes full contact with the 9 Check to make certain that a heat absor lamp mount release the lever bing filter has been inserted into the shaft in order to protect the connected light 8 Connect the plug of the lamp with the guide coupling in the instrument Ensure that no wires are touching the reflector 10 Close the housing cover and insert the ofthe lamp screws Leica MZ10 F User Manual Leica EL6000 Connection to the Fluorescence Lamp Housing Always connect the light guide to the lamp 2 Insert the adapter into the fluorescence 4 Insert the short end of the light guide into housing of the stereomicroscope in order lamp housing and retighten the locking the lamp housing of the stereomicroscope to prevent danger to the user from the high screw as far as it
46. nce microscopy takes advantage of this property Specimens without self fluorescence are stained with a fluorescent substance One such dye is green fluorescent protein GFP which is used in molecular biology Leica MZ10 F The Leica M Series The Leica M series fluorescence stereomicro scopes allow entire fluorescent specimens to be studied unprepared and in three dimensi ons The fully apochromatic optics system and the large zoom range make the Leica M series stereomicroscopes your first choice The paten ted TripleBeam third beam path for fluores cence illumination and the patented FLUOIII filter system generate highest quality fluore scence images The very bright high pressure mercury burner and specially selected filter sets enable you to distinguish the finest structures and enhance the quantity of information for incident light fluorescence User Manual The filter system The FLUOIII filter system consists of a rapid filter changer for barrier and excitation filters and a filter insert for a filter holder with your choice of filter A total of four filter sets which are labeled can be used in the rapid filter chan ger An empty filter cartridge is also available for individual filter combinations Each filter set includes two barrier filters to the visual beam paths and an excitation filter to the illumination beam path 91 Simple filter holders Simple filter holders Three simple filter holders are
47. need to be readjusted even if the maximum travel path is changed Exception If the power fails while the moto rized focus is moving the position data are lost In this case the calibration must be repeated using the Leica LAS software or the Leica SmartTouch To do so please consult the respective manual Leica MZ10 F Restricting the bottom travel range 3 Push the limit stop to the height of the 1 Move the motorized focus into the lowest motorized focus position you want to reach Itis easiest to move the limit stop by keeping the 2 Unscrew the screw of the limit stop on the screwdriver inserted and moving it upwards side of the focusing column 4 Tighten the screw of the limit stop User Manual 18 Optics Carrier Tools used Assembling the Optics Carrier Hex socket screwdriver 4 mm 1 Place the microscope carrier on the focu sing column so that the screw fits into the Please use the planapochromatic objec thread provided and the lug fits into the tives of the M series article numbers 10 groove 450 027 to 10 450 030 with the microscope carrier lf you have any inquiries regarding compatibility and adaptation of other models please contact your Leica consultant 3 Insert the optics carrier into the microscope carrier and fasten it with the locking screw 2 Press the microscope carrier backwards to the focusing column and screw it in place using your other hand Leica MZ10 F User Man
48. nge fit tightly and accurately You can extend the overall magnification range 2 Remove the plastic tube guard using available 10x 16x 25x and 40x wide field eyepieces for persons wearing glasses 4 Securely tighten the clamping screws Leica MZ10 F User Manual 22 Objective Tools used Attaching the objective 2 Screw the objective clockwise into the gt No tools required 1 Remove the protective cover on the optics optics carrier Carrier Hold the objective firmly during assembly and disassembly so that it does not fall onto the stage plate This applies particularly to the 2x planapochromatic objective which is very heavy Remove all specimens from the stage plate first Leica MZ10 F User Manual 23 UV Protection Screen Assembly Tools used Assembly 2 Fasten one side of the holder for the UV Allen key 1 Screw the adapter for holding the UV protection screen on the adapter The other protection screen to the fluorescence axis side holds the protection screen Intended use 3 Bring the holder into the correct position by turning it on its center joint Tighten the screw on the center joint to fasten the UV protection screen in the selected position in front of the objective The UV protection screen in front of the speci men plane prevents the user from looking directly into the UV rays Safety Notes UV radiation can damage the eyes There fore it is mandatory to install the UV filte
49. nification factor of the C mount adapter must match the image format of the sensor as closely as possible see table Leica MZ10 F Ifthere is unwanted shading at the corners even with a compatible C mount adapter installed it can be corrected using the Shading function of the camera software Alternatively you can also use a C mount adap ter with higher magnification This primarily avoids the critical border area of the field of vision and concentrates on the center of the field of vision Camera optimal suitable large smaller image field image field DFC290 0 5x 0 63x DFC420 0 5x 0 63x DFC490 0 63x 0 8x DFC500 0 63x 0 8x User Manual Cameras from third party suppliers In addition to Leica DFC cameras with the stan dardized C mount interfaces you can connect third party cameras to the microscope using a T2 bayonet adapter To do so instead of the C mount adapter simply use the correspon ding SLR adapter with T2 connection However these third party cameras are not integrated into the Leica Application Suite and have to be operated using the corresponding software from the camera manufacturer The Leica digital cameras are detailed in a sepa rate user manual along with instructions for their assembly and use 61 Trinocular Video Phototube 50 Use With its third beam path the trinocular video phototube 50 enables you to simultaneously view and photograph a specimen Th
50. ontrast Negative relief contrast Suitable for semitransparent and transparent Suitable for semitransparent and transparent 1 Push the path folding mirror all the way specimens The phase structures look like hills specimens These settings result in a negative back towards the column relief contrast Phase structures look like valleys The effect can be strengthened or weakened by 2 Turn the path folding mirror into the notch gently tilting the path folding mirror The effect can be strengthened or weakened by position at an angle of 45 gently tilting the path folding mirror Leica MZ10 F User Manual 77 Leica TL RCI Relief Images cont d Dynamic relief contrast Suitable for semitransparent and transparent specimens By tilting the path folding mirror slightly you can move the gaps over the entire field of view and quickly toggle between positive and nega tive relief images The dynamic effect makes it easy to distinguish phase structures from amplitude structures Leica MZ10 F Constraints The relief methods provide good results from mid zoom to high magnifications and with 1x 1 6x and 2x objectives In the lower zoom half and with weaker specimens the object field may not be uniformly illuminated We recommend using the transmitted light base with 1x or higher objectives and not objectives with a long focal length User Manual Using Filters Filters for Leica TL RC and TL RCI The transmitte
51. r and adjust it correctly Always position the UV protection screen so that the operator can never look directly at the light spot Leica MZ10 F User Manual 24 Transmitted Light Base TL ST Unpacking the base The base is delivered with the adapter plate installed Make sure the instruments are unpa cked on a flat sufficiently dimensioned and non slip surface Leica MZ10 F Focusing drive and column 1 Unscrew the extension plate from the base using the Allen key provided User Manual Attach your focusing drive column from below using the four hexagon head screws Reattach the adapter plate to its origi nal position using the six hexagon head screws Transmitted Light Base TL BFDF Before First Use Removing the transport anchors Before you can use the transmitted light base for the first time it is absolutely ne cessary to remove the two transport anchors Anchor of mirror Anchor of switching slide Leica MZ10 F User Manual 26 Transmitted Light Base TL BFDF Standard delivery The base is delivered with the adapter plate installed The selected stage Leica IsoPro Cross stage or Leica standard stage 10 447 269 and the focusing drive will have to be mounted later Stage assembly The Leica TL BFDF transmitted light base can be equipped with three different stages gt Leica IsoPro Manual Cross stage gt Leica IsoPro Automatic Cross stage Leica standard stage 10 447 26
52. rol options No AntiShock Pads Yes Dimensions WxHxD 340x430x85 mm Leica MZ10 F User Manual 102 Leica TL BFDF Transmitted Light Base Light source External via cold light source Illuminated area 40 mm Connections Connection for cold light guide active f 10 mm end tube f 13 mm Weight 5 8 kg Illumination types Bright field Yes Dark field Yes Oblique light No Relief Contrast System RC No CCIC Constant Color Intensity Control No Internal shutter lamp control Yes Integrated filter holder No Coated optics for increasing the color No temperature Matching of high num aperture Yes Remote control options Yes AntiShock Pads Yes Dimensions WxHxD 340x390x90 mm With cold light source Leica CLS150 LS Leica MZ10 F Concave mirror With external light source User Manual 103 Leica TL RC TL RCI Leica TL RC Leica TL RCI Light source External via cold light source Halogen lamp 12 V 20 W Quick illuminant change Yes Illuminated area 35mm 35mm Power supply 100 240 V frequency 50 60 Hz energy consumption 30 W max ambient temperature 10 40 C Connections Connection for cold light guide active f 10 mm end tube f 13 mm 1xUSB type A 1xUSB type B 2xCAN BUS Weight 6 0 kg 7 2 Kg Illumination types Bright fie
53. s in the positive relief cont rast like hills in the inverted relief contrast like valleys User Manual Increased contrast without relief is attained if both diaphragms are set to 45 A gap like illuminated area is created By tilting the path folding mirror slightly you can move the gap over the entire field of view and quickly toggle between positive and negative relief images The dynamic effect makes it easy to distinguish phase structures from amplitude structures 75 Leica TL RCI Methods in Transmitted Light Vertical bright field illumination Suitable for stained amplitude specimens with sufficient contrast The light beams are deflected vertically through the specimen This results in an accurate bright field with maximum brightness Leica MZ10 F Inclined transmitted light Suitable for semitransparent opaque speci mens such as foraminifera and fish eggs Move the path folding mirror until the desired data are visible User Manual Single sided dark field Suitable for fixed specimens and fine structures The flatter the angle at which the light beams are deflected into the specimen plane the darker the substrate appears A dark field like transmitted light is created Outlines fine edges and structures are bright in contrast with the dark background through diffraction of the light beams on the dark background Leica TL RCI Relief Images Starting position Positive relief c
54. s continued Supply unit Always unplug the supply unit from the power supply gt When installing and disassembling the lamp housing gt Before opening the lamp housing gt When replacing the high pressure mercury lamp and other parts such as the heat absorbing filter or the collector During maintenance work on the supply unit Leica MZ10 F Lamp housing Never open the lamp housing while the lamp is switched on Risk of explosion UV exposure blinding Before opening the lamp housing allow it to cool off for at least 15 minutes Danger of explosion Never cover the air duct on the lamp housing Fire hazard User Manual Mercury lamp p Follow the user manual and safety instruc tions provided by the lamp manufactu rer and in particular the section on how to proceed if a lamp breaks and releases mercury For transport remove the mercury lamp transport it in its original packaging and protect moving parts in the lamp housing using the transport anchors When it has reached the end of its rated life follow the manufacturer s specifications and the minute meter on the supply unit To minimize the risk of explosion replace a discolored mercury lamp promptly Leica Microsystems assumes no liability for damage caused by exploding incor rectly installed or improperly used mercury lamps Table of Contents Assembly Quick Start Guide
55. scence related parts referto Page 89 C Assembly Assembling the Focusing Column with a Transmitted Light Base The first step is to connect the focusing column Assembling the column adapter Assembling the focusing column ofthe M series to the corresponding base 1 Securely install the column adapter on the 2 Securely screw the focusing column to the column using the four included screws base using the six included screws Tools used Hex socket screwdriver 3 mm Leica MZ10 F User Manual 16 Assembling the Focusing Column with an Incident Light Base When using an incident light base the focu Assembly 3 Screw the base securely onto the focusing sing column and motorized focus are installed 1 Place the focusing column on the side drive directly on the base no extension plate is requi red Tools used gt Hex socket screwdriver 3 mm 2 Insert the four screws provided into the outer holes of the base Leica MZ10 F User Manual Motorized Focus Restricting the Travel Path Depending on the work situation it may be necessary to restrict the maxi mum travel path of the stereomicroscope This prevents the following Injuries when manipulating the specimen because the fingers or hand get pinched Accidental contact between the objective and the specimen and potential resulting damage Readjusting the motorized focus The motorized focus is factory adjusted and normally does not
56. the Instrument continued Compatible light guides The Leica EL6000 compact light source is compatible with liquid light guides that are compatible with a Storz long light inlet and whose transmission curve fits the lamp used Where applicable refer to the lamp s data sheet for its spectral light distribution When connecting the light guide to the light source or the microscope adapter make sure not to kink or damage the light guide Your Leica consultant will be glad to provide additional information about suitable light guides Leica MZ10 F Identification of the instrument The nameplate of the instrument is on its bottom It includes the necessary information for identifying the instrument User Manual The Leica EL6000 compact light source complies with Council Directive 98 79 EC concerning in vitro diagnostics It also conforms to the Coun cil Directives 73 23 EEC concerning electrical apparatus and 89 336 EEC concerning electro magnetic compatibility for use in an industrial environment 84 Leica EL6000 Operation Controls Power switch power indicator Reset button for running time meter Running time meter Shutter control Intensity switch Mm bh WU N Leica MZ10 F Running time meter The running time meter measures the burning time of the lamp not the switch on time of the Leica EL6000 compact light source The running time meter displays its current status in this format ho
57. ttings 10 450 023 16x 5 diopter settings 10 450 024 25x 5 diopter settings 10 450 025 40x 5 diopter settings 10 450 026 Leica MZ10 F User Manual 54 Health Notes Potential sources of infection Direct contact with eyepieces is a poten tial transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using individual eyepieces or detachable eyecups Eyecups can be ordered separately Please contact your Leica partner Leica MZ10 F a 10 23 P aI 29 de y 4 MIA 43 HA ZI A SICA 10450 4 Cica 0460 Separate eyecups are an effective way of preventing infections User Manual Dioptric Correction All Leica eyepieces are also available with built in dioptric correction allowing the microscope to be used without glasses even by those with vision problems The correction comprises 5 diopter settings Leica MZ10 F Using the Dioptric Correction 1 Set the dioptric correction of both eyepi eces to the mid position 0 diopter settings While wearing your glasses look through the eyepieces and focus on the specimen Rotate both eyepieces to the maximum value of 5 Hold one eye closed and rotate the other eyepiece in the direction until the speci men appears sharp Then open the other eye and correct the diopter settings until the image is uniformly sharp User Manual Note that when using dioptric correction the advantage
58. u the best of success in your work after all you are now equipped with the best tool User Manual 12 The Modular Design Everything is Relative The Leica M series provides maximum flexibility in choosing equipment thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades The optics carriers eyepieces bases and more can be combined in any way you choose allowing you to create the microscope that best suits your needs Leica MZ10 F Despite this you will notice that the control elements and individual components do not differ significantly Whichever configuration you choose you will quickly feel right at home User Manual Have a special request Let us know Leica Microsystems enjoys an exceptional repu tation when it comes to devising customer specific solutions If you have a special request that cannot be met with standard parts contact your Leica consultant We have a solution for every problem On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant click here to skip through the instal lation instructions and go directly to the Quick Start Guide on Page 43 Leica MZ10 F If on the other hand you are assembling the microscope yourself continue with the Assem bly chapter which begins on Page 15 User Manual For everything you need to know about the correct use of fluore
59. ual 19 Iris Diaphragm The optional iris diaphragm which can be attached between the optics carrier and tube fulfills the same purpose as those in a camera It regulates the available light which changes the depth of field The depth of field or depth of focus is the area of a specimen that is brought into sharp focus Leica MZ10 F Installing the iris diaphragm Place the iris diaphragm on the optics carrier and tighten the locking screw 1 User Manual Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner A Please note that inserting intermedi ate elements can lead to shading in the border area of the field of view Preparations 1 Unscrew the positioning screw and remove the protective cover Leica MZ10 F Assembling the tube Push the tube for example the inclined binocular tube into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove 2 User Manual 3 While holding the tube only slightly care fully tighten the positioning screw It is automatically brought to the correct posi tion Eyepieces Tools used Preparation Inserting the eyepieces gt No tools required 1 If you want to use an optional graticule 3 Push the eyepieces into the tubes as far as insert it now Page 58 they will go and check to ensure that they Magnification ra
60. urs minutes 1 Press the reset button to set the running time meter of the lamp to 0 The running time meter should be reset after every lamp replacement so that it always displays the current accurate lamp life Discontinue using the built in lamp when it reaches the end of the life specified by the manufacturer User Manual Shutter control gt In the open position the shutter of the Leica EL6000 is open regardless of the signal from the remote control If the shutter control is in the remote posi tion the shutter is controlled by the remote control Intensity switch The intensity switch provides optical dimming of the light in five fixed increments 85 Leica EL6000 Troubleshooting Fault description Power indicator is not illuminated after being switched on instrument shows no function Power switch is illuminated after being switched on instrument shows no function Target system receives too little light or none at all Possible faults No power supply voltage present connecting cable not plugged in correctly defective Remedy Check power supply voltage and connecting cable Fuses in instrument defective Housing cover loose or removed Replace fuses use permitted replacement type only Attach housing cover securely Lamp worn out Shutter closed or intensity switch at left limit stop Light guide not inserted into light guide output properly Replace lamp
61. val keep all the transport anchors in the plastic bag provided for future transport Leica MZ10 F User Manual 32 Leica IsoPro Motorized Mechanical Stage Assembly cont d The bases The TL BFDF TL RC and RCI transmitted light bases can be equipped with three different stages Standard stage manual and automated IsoPro mechanical stage The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base without the stage mounted Disassembly is performed in reverse order of the following steps Leica MZ10 F Cross stage and base Never move the sledge of the motorized cross stage manually in the X direction as otherwise the mechanical system will be damaged 1 Place the mechanical stage onto the base 2 Pull the upper part of the mechanical stage carefully towards the user and fasten the lower part onto the transmitted light base 3 Attach the mechanical stage evenly to the three threaded holes 4 Now move the mechanical stage as far as it will go in the direction of the column 5 Insert the glass plate into the mechanical stage User Manual Leica IsoPro Motorized Mechanical Stage Assembly cont d Cross stage for X Y stage control unit into the power supply the cross stage initiali 1 Plug the CTL2 plug of the mechanical stage zes and automatically drives to the
62. will go energy light 5 Securely tighten the clamping screw 1 Unscrew the positioning screw and remove the protective cover of the fluorescence lamp housing 3 Unscrew the three red protective caps on the light guide Leica MZ10 F User Manual 41 Leica EL6000 Connection to the Fluorescence Lamp Housing continued 6 Insert the long end of the light guide into Only use a light guide with a light input of the light output of the Leica EL6000 There the type Storz long as otherwise damage must be a noticeable click to the instrument and danger to the user can result risk of being dazzled Before you open the shutter the light guide iL must be connected on both sides Other II wise the escaping light can cause injury to eyes and skin and damage to furniture Never look into the light escaping from the light guide 5 Connect the Leica EL6000 to the power system using the power cable Leica MZ10 F User Manual 42 Quick Start Guide An Overview of the Leica MZ10 F Eyepieces with dioptric correction Binocular tube Fluorescence housing Filter Changer Magnification zoom Leica MZ10 F Objective UV protection screen Transmitted light base Focus column 11 UV Shutter 12 System Illumination EL6000 O 0 N Si i VI ph UU N o Leica MZ10 F User Manual 44 The Correct Interpupillary Distance The interpupillary distance is correctly set if you see a s

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