Opti-Check™ - Turner Designs
Contents
1. Contact Customer Support support turnerdesigns com to receive a RMA for service Opti Check User s Manual 13 7 Warranty 7 1 Terms Turner Designs warrants the Opti Check Fluorometer and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the data of shipment from Turner Designs with the following restrictions e Turner Designs is not responsible for replacing parts damaged by accident or neglect Your instrument must be installed according to instructions in the User s Manual Damage from corrosion is not covered Damage caused by customer modification of the instrument is not covered e This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for accidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state e Damage incurred in shipping is not covered 7 2 Warranty Service If you purchased your Opti Check Fluorometer from one of our partners you need to contact them for service If you purchased your instrument directly from Turner Designs and need to obtain service during the warranty period please take the following steps 7 2 1 Write email or call the Turner Designs Technical Support department and describe as precisely a2. User s Manual The Opti Check can be calibrated using a Primary standard A Primary Standard is one that contains the same fluorescent material that you are measuring in your unknown samples The Opti Check will give an actual quantitative concentration reading when a primary standard of known concentration is used for calibration The standard and samples must be in the linear detection range to get accurate quantitative results Refer to Section 5 3 for more details about the linear range and quenching of the samples For Dye tracing applications the calibration is normally performed with a primary standard made from the same dye that is being used for the testing The primary standard will either be made to a known concentration typically in ppb or ug L units or to a Known dilution factor Ideally the Primary Standard and Blank samples used for calibrating will be made with the same water the tests are being performed in 3 4 Calibration Procedure It is recommended for best accuracy that you always calibrate before performing your sample analysis The Opti Check will save the calibration settings for each channel until a new calibration is performed If the temperature of your samples or the Opti Check changes significantly the readings may show a small shift and in this case you should consider recalibrating The Opti Check is designed with ambient light rejection The black sample compartment cover does NOT need to be close
3. 2 3 Cuvette information Cuvette type Size Usage i PaartNow 10 mm Square Required for UV lt 400 nm works for all applications moMac ale PASU height 44 5 mm Do NOT use ai solvents Ronen Opti Check User s Manual 5 3 Instrument Operation and Calibration 3 1 Instrument Power Up To turn on the Opti Check press the lt ON OFF gt button After a 5 second warm up the Opti Check is ready for operation Pressing the lt ON OFF gt button again will turn the unit off or if left idle for 3 minutes the unit will turn itself off to save battery power A set of new batteries will last for over 1 000 sample readings If the batteries have low power or are not positioned properly the following warning message will be displayed Batt Level lt 20 Caution The contrast of the display can change with temperature and will get lighter as the unit gets colder The contrast can be easily adjusted Press the arrow button to darken the contrast and press the 4 arrow to lighten the display contrast 3 2 Detection Channel Configuration The Opti Check can be configured with one or two optical channels To identify the configuration look at the label on the back side of the Opti Check The lt A B gt button allows the user to toggle between the 2 channels The display will show a label in the lower left corner of the Home screen to identify which channel is activated 3 3 Calibration Overview Opti Check
4. within 10 seconds you will be asked if you want to abort the calibration Press the or 4 arrow button to abort or accept the calibration respectively If at anytime during steps 1 4 you want to stop the calibration press lt ESC gt This will return you to the Home screen and will default the instrument to the previous Calibration Opti Check User s Manual 7 3 4 3 Calibration Check Once the Opti Check calibration is accepted press the lt DIAG gt button to see what your standard to blank ratio is The standard to blank ratio is defined as the FS blank as compared to the FS standard The FS signal should be at least 5 times larger than the FS Blank It should be between 5 and 30 e If your FS std FS blk ratio is greater than 30 dilute your Calibration Standard accordingly and recalibrate Check the ratio again e f your ratio is less than 5 you ll have to find a different standard for calibration e If your ratio falls between 5 and 30 after the initial calibration then you can start measuring samples 3 0 Sample Analysis 1 Insert your sample The Opti Check is designed with ambient light rejection The black sample compartment cover does NOT need to be closed when reading samples The orientation and cleanliness of the cuvettes can have an impact on the accuracy of your results Refer to Section 5 for more details 2 Press the lt READ gt button The instrument will measure and average the fluorescence signa
5. TURNER e DESIGNS Reliable Instruments for an Unreliable Worle Opti Check Handheld Fluorometer November 11 2011 P N 998 0861 Revision A TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 Table of Contents 1 Introduction 1 1 Description 1 2 Quick View Diagram 1 3 Inspection and Setup 1 4 General Information Precautions amp Cleaning Instrument Specifications 2 1 General Specifications 2 2 Optical Configurations for Applications 2 3 Cuvette Information Instrument Operation and Calibration 3 1 Instrument Power Up 3 2 Detection Channel Configuration 3 3 Calibration Overview 3 4 Calibration Procedure 320 Sample Analysis 3 6 Diagnostic Information Sample Analysis Guidelines 4 1 Handling Samples 4 2 Positioning Samples 4 3 Linear Range and Quenching 4 4 Temperature Considerations 4 5 Data Quality Applications 5 1 Fluorescein 5 2 PTSA Quick Diagnosis Guides 6 1 Power Battery Screen Issues 6 2 Reading and Calibration Issues Warranty 7 1 Terms 7 2 Warranty Service 7 3 Out of Warranty Service Opti Check User s Manual o1o1 OI RWW WwW o CO N O OD i OOO O 11 12 13 14 15 1 Introduction 1 1 Description The Opti Check is a small lightweight highly durable handheld fluorometer ideal for quick measurements in the field Dual channel capability enables measurement for both cooling tower and boiler
6. d when calibrating or reading samples The orientation and cleanliness of the cuvettes can have an impact on the accuracy of your results Refer to Section 4 Sample Analysis Guidelines for information to help ensure the best results for your analysis 3 4 1 Assign a Calibration Standard Value This defines the numeric value that you want the standard to read For example if you calibrate with a primary standard that has a concentration of 100ppb then you will set the value to 100 and the implied unit of measure will be in ppb 3 4 1 1 Press the lt STD VAL gt button 3 4 1 2 Use the and 4 arrow buttons to set the standard value Pressing the arrow button once quickly will make a small incremental change to the value Holding either arrow button will allow you to change the value using fast scrolling 3 4 1 3 When finished Press the lt ENT gt or lt ESC gt button to accept the value and to return to the Home screen 3 4 2 Perform the Calibration 3 4 2 1 Press the lt CAL gt button 3 4 2 1 Press lt ENT gt to start the calibration 3 4 2 3 Insert your blank sample and press lt ENT gt The Opti Check will average the reading for 10 seconds and set the blanking zero point 3 4 2 4 Insert the standard sample and press lt ENT gt The reading is averaged for 10 seconds and the Standard Calibration value is set 3 4 2 5 Press lt ENT gt when the calibration is complete to accept the calibration If lt ENT gt is not pressed
7. dard 400 ppb Opti Check User s Manual 11 6 Quick Diagnosis Guide 6 1 Power Battery Screen Issues The Opti Check is generally a trouble free solid state instrument However there have been a few issues reported that can easily be diagnosed and or resolved in the field Please use this guide to address some common Power Battery Screen Issues If you are Opti Check does not experiencing appear to turn on any of the issues on the left Opti Check screen is not illuminated Replace batteries with new batteries There does not appear to be light visible inside the sample compartment when the READ button is pressed You may use your Opti Check and proceed with calibration sampling Is the issue resolved After replacing the batteries the screen is not illuminated The following message displays Batt Level lt 20 Caution Press and hold the button to darken The unit is on and when READ button is pressed the instrument powers off Opti Check screen displays unusual characters the display contrast Does this correct the issue After replacing the batteries the screen is full of black boxes Press and hold the button to lighten the display contrast Contact Customer Support support turnerdesigns com to receive a RMA for service Opti Check User s Manual 6 2 Reading and Calibration Issu
8. e change in concentration At concentrations 10 times higher than the upper limit the readings will start to decrease even though the sample concentration is increasing This effect is known as sample quenching and is due to light absorption losses in the sample See the Figure 1 graph below Opti Check User s Manual 9 When you start to see visual color in the sample this is an indicator that the sample may be above the upper limit of linearity The linearity can be verified by diluting a sample 1 1 or another convenient dilution ratio If the sample is within the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the original sample concentration was above the linear range Cal Standard Value Numeric value assigned to represent the signal level detected from the Solid Secondary Calibration Standard Blanking Level Blanking is done usinga P filtered sample of the water F being tested Distilled water Bank Calibration i can be used for in vivo Standard Concentration chlorophyll measurements X since readings are semi quantitative f or Calbration Standard gt 3 1 Blanking Level Valid Calibration Blank Ratio Figure 1 Linearity and Calibration Response Curve 4 4 Temperature Considerations Fluorescence is temperature sensitive As the temperature of the sample increases the flu
9. es Please use this guide to address some common Reading Calibration Issues When reading the blank there is negative result lt 2 or positive result gt 2 and never 0 Readings for the sample and or blank are highly variable Readings for the sample are 0 If you are experiencing any of the issues above try the following Check the sample well and make sure that it is dry Dry sample well if necessary using a non abrasive wipe or lint free cloth Wipe down the cuvettes with a lint free cloth and make sure they do not have moisture on the outside Check the cuvettes for scratches Replace cuvette if scratched Make sure that your cuvette is at least 2 3 full with solution Tap the bottom of the cuvette to purge any air bubbles from solution that may cause interference Mark the cuvette near the top to make sure cuvette position doesn t change Was the issue resolved Yes Check your FS std FS blk ratio me You may use your by pressing the lt DIAG gt button Opti Check and proceed with calibration sampling Your FS std FS blk ratio is greater than 30 Your FS std FS blk ratio is less than 5 Dilute your calibration standard by 2x and Use a higher standard concentration and recalibrate Check the ratio again recalibrate Check the ratio again Is your FSstd FSbIk ratio now between 5 30 Yes I ve tried multiple times and can t make it work
10. l for 5 seconds 3 The reading result will be displayed on the top line of the Home screen 4 The top left corner will then display WAIT for 5 seconds Once WAIT disappears another sample reading can be performed 3 6 Diagnostic Information _ Press lt DIAG gt to access the diagnostic screens 2 Press lt ENT gt to toggle to the FS Full Scale values for the blank BIk and standard STD calibration points The STD value should be at least 5 times larger than the Blk value to ensure consistent results This is often referred to as the Standard to Blank Ratio 3 Press lt ESC gt when finished to return to the Home screen Opti Check User s Manual 8 4 Sample Analysis Guidelines 4 1 Handling Samples 4 1 1 Take care not to spill samples into the sample chamber Wipe up any spills promptly 4 1 2 The cuvette MUST BE DRY on the outside when taking readings Any moisture or condensation on the outside of the cuvette can affect the reading 4 1 3 Fill the cuvette with at least 2mL solution volume or at least 2 3 full Significant error in the readings can result if the cuvette contains less than this minimum volume 4 1 4 The Opti Check is very sensitive and even small amounts of material from a previous sample may contaminate the sample and result in errors Use a clean cuvette for all readings If you are using the same cuvette for your samples it is very important that you thoroughly clean the cuvette bet
11. orescence decreases For best accuracy make sure your samples are at the same temperature as the calibration standard If you have the ability to measure sample temperature a temperature coefficient may be applied to your measurements see section 2 2 4 5 Data Quality The Opti Check is only as accurate as the standards that are used to calibrate it This is why it is important to take care when preparing standards samples and blank One should follow good laboratory practices when preparing all solutions and samples Opti Check User s Manual 10 5 Applications 5 1 Fluorescein Fluorescein is a fluorescent tracer dye that provides an accurate cost effective method for monitoring industrial boiler applications Fluorescein dye can be detected using the Blue channel on the Opti Check between 485 540nm The linear detection range is 0 to 400 ppb active ingredient in potable water Turner Designs offers Fluorescein Dye PN 10 508 Calibration standard 10 ppb and Fluorescein Dye PN 10 509 Calibration standard 400 ppb 5 2 PTSA PTSA is a fluorescent tracer dye that measures in the UV range on the Opti Check between 365 400nm It can provide an accurate cost effective method for monitoring industrial cooling tower applications and it can also be used as a standard for UV sensors The linear detection range is 0 to 1000 ppb Turner Designs offers PTSA PN 10 608 Calibration standard 100 ppb and PTSA PN 10 609 Calibration stan
12. s possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com 7 2 2 Carry out any adjustments or tests as suggested by the Technical Support Department 7 2 3 If proper performance is not obtained you will be issued a Return Authorization number RMA Package the unit write the RMA number on the outside of the shipping carton and ship the instrument prepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be repaired and returned free of charge for all customers in the contiguous continental United States Opti Check User s Manual 14 7 3 Out of Warranty Service Follow steps for Warranty Service as listed above If our Technical Support department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs 845 W Maude Avenue Sunnyvale CA 94085 Opti Check User s Manual 15
13. system applications Simple to operate the Opti Check is a perfect tool for system verification The Opti Check can be configured with two channels Fluorescein PTSA or both 1 2 Quick View Diagrams 4 MO GLASS CUVETTES o 1 3 Inspection and Setup 1 3 1 Inspection Upon receiving your instrument please inspect everything carefully and make sure all accessories are present All shipments include The Opti Check The User s Manual on CD 4 AAA batteries Cuvettes Qty 4 Storage Pouch Opti Check User s Manual 3 1 3 2 Setup Before the Opti Check can be used the supplied batteries must be installed 1 2 3 4 On the back side of the instrument remove the battery panel by loosening the center screw and then gently pulling on the screw There is some resistance due to the o ring which is used for watertight sealing see Section 1 2 for diagram Install the 4 AAA batteries and verify the batteries are positioned correctly Replace the battery panel and press it down into position Hold the battery panel down in place and gently tighten the screw until it is snug Do not over tighten the screw Note Ifthe battery panel is very difficult to press down or remove you can apply a small amount of silicon based grease to lubricate the o ring as needed 1 4 General Information Precautions and Cleaning Opti Check User s Manual The sample compartment cannot accept 10 mm square cuvettes because the cuve
14. tte wall thickness exceeds the 12 mm maximum outer dimension that the Opti Check can accept The 10 mm size is the internal dimension of the cuvette and some plastic cuvettes may be too large Do not force oversized cuvettes into the sample compartment This can damage the sample compartment If the cuvette does not easily fit down inside the Opti Check you will need to purchase different cuvettes Use caution around solvents because they may attack the plastic case of the Opti Check If a sample is accidentally spilled inside the Sample Compartment invert the Opti Check to drain out the excess liquid Then wipe the inside area dry with a clean soft towel or tissue If extra cleaning is needed use a mild detergent to dampen the towel for cleaning Do not submerge the Opti Check in water Do not expose the Opti Check to temperatures outside the specified range of 5 to 40 C or damage may occur to the unit that will not be covered under warranty 2 Instrument Specifications 2 1 General Specifications 4 45cm x 8 9cm x 18 4cm LCD Display 2 x 16 characters Meets IP 67 Standard dustproof and watertight 2 2 Optical Configurations for Application Channel E Minimum F Temperature Application Light Source Detection Limit Linear Range Excitation Emission Coefficients Fluorescein 0 0036 C Blue LED lt 1 ppb 0 400 ppb 485 nm 540 nm Exponential 0 00126 C PTSA UV LED lt 1 ppb 0 1000 ppb 365 nm 400 nm Exponential
15. ween samples A good way to confirm the cuvette cleanliness is to read a blank solution If the reading is higher than the normal blank reading the cuvette is not clean 4 1 5 Any bubbles in the sample will affect the readings Take care not to introduce bubbles into samples Remove any bubbles by lightly tapping with your finger on the outside cuvette wall or cover the top of the cuvette and tilt the sample to help dissipate bubbles 4 2 Positioning Samples The orientation of the cuvette in the sample compartment can give slightly different readings especially for low concentration samples This is due to variations in the walls of the cuvette that are not readily visible to the eye We recommend that the cuvette be marked at the top on one side and positioned in the sample compartment the same way each time for best results 4 3 Linear Range and Quenching The linear range is the concentration range in which the readout of the Opti Check is directly proportional to the concentration of the fluorophore in the sample The linear range begins with the lowest detectable concentration and spans to an upper limit concentration that is dependent on the properties of the fluorescent material and the cuvette optical path length For Fluorescein dye in the 10 mm square cuvette the upper limit of linearity is approximately 400 ppb At concentrations above this upper limit the fluorescence reading will not increase at a linear rate in comparison to th
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