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II. Configuring the MAGIC 2002

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1. MAGIC Pump Configuration fessure Units LOAD Event INJECT Event Pump Com Port COM 3 z Pump B Com Port COM 4 z Max pressure in PSI 5200 AMin pressure in PSI P After entering all of the data click on OK PSI x Load z Inject 7 59 Enter the minimum operating pressure The pumps will shut off if the pressure drops below this value Entering a value of 0 disables this function Select the LOAD Event This should always be set to Load Select the INJECT Event This should always be set to Iniect Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 45 MAGIC Detector Configuration Screen Select COM 5 for Click on OK after selecting the Detector Com the Detector Com Port Port MAGIC Detector Configuration Detector Com Port com 5 z Figure 46 MAGIC Temperature Controller Configuration Screen Select COM 6 for the Click on OK after select the Temperature Controller Temperature Controller Com Com Port Port Controller Com Port feom 6 z Figure 47 Detector Configuration Screen UV 1 Enter UV 1 for the Detector Name Select Analog for the Detector Model Detector Configuration Detector Name Juv 1 Select SS420 for the aT Click on this symbol Acquisition Source ah and select Acquisition Source s
2. lamps on of wavelengths AGIC Temp Controller MAGIQ Pump Extemal Events MAGIC2fetector or off when Status Enabled is Status Enabled Manual Set checked Allows setting of number Number of wavelengths AutoZero V Deuterium On of wavelengths Dual UV 190 450 nm when used with guning Tungsten On This box shows when we Manual Set Wavelength Absorption Sample Energy Reference Energy the detect utton e detector pa 0 00000 is running a method Displays or sets ms oe Must have the primary Status wavelength Enabled information checked Displays primary and Displays primary and Displays primary and secondary absorbance in secondary sample secondary reference real time when Status energy when Status energy when Status Enabled is checked Enabled is checked Enabled is checked Displays or sets the secondary wavelength information 35 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com III System Shutdown When the system is not in use an orderly shutdown procedure should be followed to help maintain the instrument Daily Shutdown At the end of the day run the shutdown procedure If a group of samples is being analyzed simply attach the shutdown procedure to the end of the sequence The Shutdown procedure allows the pumps to flow at a rate of 10 ul min at 50 B with the detector lamps off Short Term
3. A rtisan Artisan Technology Group is your source for quality TecmoogyGroup new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment 7 EQUIPMENT DEMOS HUNDREDS OF InstraV ea REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED s Z tia Contact us 888 88 SOURCE sales artisantg com www artisantg com Michrom BioResources Inc 1945 Industrial Drive Aubum CA 95603 TEL 530 888 6498 FAX 530 888 8295 Micro Analytical Gradient Integrated Chromatograph 2002 MAGIC 2002 Operator s Manual Version 2 0 Part No 602 00000 00 1 99 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com TABLE OF CONTENTS Page Warranty amp Service Agreement Information U I a 5 Safety Information ic aaa 6 System Requirements n d a IDD au aaa 9 L
4. 56 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 40 EZChrom Elite Client Server Screen Right mouse click on the new instrument icon and select Configure EZChrom Elite Client Server a alle x se sl 2 P The Enterprise Figure 41 Instrument Configuration Screen Type in the name of the instrument MAGIC 2002 Click on Configure Select MAGIC for the type of instrument Geryernene 57 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 42 MAGIC Configuration Screen Double clicking on each icon on the Available Modules side will move them over to MAGIC Configuration the Configured Modules side Double clicking on each icon on the Configured Modules side will open the configuration screen for each module MAGIC Pump MAGIC Pump MAGIC MAGIC Detector Temperatu ais OF H Event MAGIC Event Detector Detector Configuration Detector ars iB Detector Detector Temperatu Analysis Options Auto Configuration Cancel Help Move all of the Modules from the Available module side to the Configured module side Move three additional detector modules as shown above one for each of the four channels of A D input available with the MAGIC Elite data system Configure the Event Configura
5. Instrument Setup 20 000 21 000 Figure 17 Instrument Setup of Data Acquisition Channels Screen Same for all channels Select to turn on the data acquisition channel for the method run Instrument Setup Enter the Select the amount of sample rate for r E e a Iw iEa time you data want to acquisition delay the Faster sample mec Z start of the rates larger data data files more acquisition resolution after a trigger has been Enter the received amount of time to acquire data Note The screens for data acquisition will only be displayed in the method if the data system option was purchased as part of the MAGIC control software 29 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 18 Instrument Setup of Detector Screen Select which lamp is on for the method run Note If you select Dual Vis for number of wavelengths you must have both lamps on Select this if you want the detector to auto zero ona wavelength change during the method run Enter the time and wavelength for the method run Normally this will be set at time zero with no changes during the method run Instrument Setup M E s s M Dual UV 190 450 nm Po Select the number of wavelengths for the method run Choices are Single Dual UV or Dual
6. does not fit into the grove in the cap insert it should be removed and replaced with a new o ring If the swollen oversized o ring is not torn or cut it can be stored in a drawer and will return to its original size in a few days The MAGIC 2002 is shipped with 3 spare o rings Turn on the Helium source to the instrument Helium should be regulated to the instrument between 20 and 200 PSI This pressure is then regulated within the instrument to 6 PSI Please note that once the Helium source is on Helium will be delivered to the wash Bottle To allow Helium to be delivered to the Solvent A and Solvent B bottles open the Helium valve located above the solvent bottles Refer to Figure 5 page 16 To open the valve place the valve in the On position A moderate bubbling of gas should be observed in the A and B bottles with the opening of the Helium valve Prior to pressurizing the solvent bottles sparging should be performed To sparge the bottles degas solvents simply select Sparge Vent on the Sparge Vent valve next to the Helium On Off valve After one to three minutes of sparging the Sparge Vent valve should then be turned to Pressurize While the solvent bottles are pressurizing the instrument should be checked for Helium leaks Check for gas leaks around the solvent bottle caps and all associated fittings Also check for Helium leaks around the inlet fitting to the instrument the Helium On Off valve and the Sparge Vent valve The sy
7. unless otherwise specified If the instrument needs to be changed to 230V AC remove the power cord to the instrument Remove the voltage selection switch block using a small screwdriver Change the fuses so that a 2 0A fuse is on the side labeled 110V 120V and a 2 5A fuse is on the side labeled 220V 240V Insert the voltage selection block so that the white down arrow under the 220V 240V lines up with the white up arrow on the voltage selection socket on the MAGIC 2002 Switch the voltage select switch to the 230V AC setting The MAGIC 2002 can run at 100V AC by keeping the original fuses in place at the 115V AC setting and simply changing the setting on the back of the UV Vis Detector from 120V AC to 100V AC 40 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com CHAPTER 3 OPTIONS I Selecting Flow Range Kits for Optimum HPLC Conditions Gradient delay volume equilibration volume sample injection volume and extra column volume can be optimized using the MAGIC 2002 to achieve the true theoretical gains in sensitivity with microbore and capillary columns The MAGIC 2002 has been especially designed to be flexible for changing between analytical 100 5000u1 min microbore 20 1000ul min and capillary flows 0 1 20ul min with the use of Flow Range Kits Analytical Microbore or Capillary Each Flow Range Kit optimizes the system for that particular flow range to allow for the
8. 3 Disk At least 80 MB free disk space Note Service packs and DCOM95 are available on the Elite 2 1 CD ROM Note Do not use Open GL Screen Savers with EZChrom Elite MAGIC 2002 HPLC Control System OR MAGIC 2002 HPLC Control Data System Analytical Flow Range Kit OR Microbore Flow Range Kit OR Capillary Flow Range Kit Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com LIST OF SPARE PARTS AND CONSUMMABLES SPARE PARTS Part Number 004 32012 01 004 61027 00 004 32033 00 003 5 1003 00 003 5 1004 00 003 5 1005 00 003 5 1006 00 CONSUMMABLES Part Number 602 25011 99 602 25027 88 602 25028 88 200 39000 00 004 25 106 00 004 25131 88 004 25003 00 004 25004 00 004 25005 00 004 25006 00 004 25027 00 004 65023 00 004 25094 00 004 25008 00 004 25009 00 004 32001 00 004 32002 00 004 32004 00 Description O ring Viton Bottle 1000mL Plastic coated Bottle Nalgene 2L Fuse GMA 5x20 5A 250VAC Fuse GMA 5x20 3 5A 250VAC Fuse GMA 5x20 2 5A 250VAC Fuse GMA 5x20 2A 250VAC Description Pump head Rebuild Replacement Check Valve Inlet Replacement Check Valve Outlet Replacement Deuterium Lamp 2u Titanium Filer Assy Repair Kit Helium Check valve Assembly Upchurch 1 16 SS Male Nut Upchurch 1 16 SS Ferrule Valco 1 16 SS Male Nut Valco 1 16 SS Ferrule Union ZDV Peek 10 32 Upchurch 10 32 Delrin Plug Optimize 10 32 Delrin
9. Continued Plugged tubing in the Helium system Isolate the plugged tubing and clear the blockage or replace the tube No pressure with flow set in the Magic control software One or both purge valves are open to the waste compartment Close the purge valve by turning clockwise until tight No analytical column is installed with column inlet tubing attached Install analytical column and attach column inlet tubing to head of column There is a leak at a fitting on a pump head purge valve or the 10 port valve Shut flow off to pumps via the control software Turn solvent selection valves to the vertical or Off position Remove the fitting and thoroughly dry ferrule and ferrule seat using a Kimwipe Retighten fitting Pump head check valve s failed Replace check valve s with new or rebuilt check valve s Pump head seals are worn or scored Run pumps isocratically in wash solvent at 50 l min 5 pump B for 30 minutes Save pressure data Now run pumps isocratically in Wash solvent at 50 ul min 95 pump B for 30 minutes Save pressure data Fax data to Michrom BioResources Inc for diagnosis by technical support personnel Pump head piston s are worn or scored Operate pumps isocratically in wash solvent at 50 l min 5 pump B for 30 minutes Save pressure data Now run pumps isocratically in wash solvent at 50 l min 95 pump B for 30 minutes Save p
10. EZChrom Elite Client Server Screen To configure the hardware click on Tools select Interface Configuration ys EZChrom Elite Client__ srver D xj File Edit View BEES ES HESL aj as v System Administration Mode Curt System Administration Wizard ale at Options is MEE S aN PG Se ney aah ad rk Naot a feat ee a Displays the instrument interface configuration Figure 37 Interface Configuration Screen Highlight the SS420 A D icon and click on Properties Interface Configuration E SOND Help 55 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 38 SS420 Configuration Screen Select Board 1 Enter the Base address Normally set to 360 Select the Interrupt level This must match the switch settings on the SS420 board After entering all of the data click on OK to exit Select the Base frequency Normally set to 10 Select the full scale range for each channel Normally set to 0 1 v After setting the Interface configuration the computer must be restarted for the new hardware settings to take effect When the computer restarts click on Start go to Programs go to Chromatography and select EZChrom Elite Figure 39 EZChrom Elite Client Server Screen Select New Instrument EZChrom Elite Client Server x sil ss Sel
11. Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 50 Detector Configuration Screen Pressure Enter Pressure for the Detector Name Select Analog for the Detector Model Detector Configuration Detector Name Pressure oe a SQ symbol Select SS420 for the Acquistion Source 55420 3 ee Sek 1 Acquisition Source Pst Analog Input Chan D Enter PSI for the Y Axis Units Click on OK after entering all of the data Enter 147 for the Y Axis Multiplier After all eight modules have been configured click OK on the MAGIC Configuration screen to save these configurations and return to the MAGIC EZChrom Elite Client Server screen Double click on the MAGIC 2002 icon to open the MAGIC software 62 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com HI Configuring the MAGIC 2002 Instrument for Control Only After installation of the EZChrom Elite and MAGIC 2002 software it is necessary to configure the computer hardware To configure the MAGIC 2002 hardware first open EZChrom Elite by clicking on Start going to Programs going to Chromatography and selecting EZChrom Elite Figures 51 through 61 present step by step instructions for configuring the computer for instrument control Figure 51 EZChrom Elite Client Server Screen To configure the hardware click on Tools select I
12. Purging Pumps to Remove Air 38 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 28 Pump head REMOVE Do not remove Allen screws Allen screws Do not remove P Do let remove REMOVE Replacing Detector Lamps Prior to changing either detector lamp D2 or Tungsten close the controller software and power the instrument off Remove the cover from the UV lamp compartment as shown on page 21 Refer to Figure 11 page 22 Disconnect the appropriate plug from the connector and remove the lamp by unscrewing the associated thumb screw s Replace the lamp reconnect the plug to the connector and place the cover back on the UV lamp compartment The instrument can now be powered on V Re Installing The MAGIC 2002 If an instrument has been moved and or needs to be installed by someone other than a Michrom BioResources Inc specialist follow the procedure below for Helium connection and power connection Refer to the section in this manual entitled System Startup Chapter 2 Section 1 page 23 for guidelines to getting the system operational 39 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Helium Helium must be connected to the MAGIC 2002 for proper solvent delivery to the pumps Helium is connected to the rear panel of the MAGIC using a 1 8 i
13. Shutdown If the instrument will be shutdown for a few days or if running solvents that are too corrosive to leave in the pumps or column run the shutdown procedure using wash solvent rather than solvents A amp B Long Term Shutdown If the instrument is to be shutdown for more than a few days a more extensive shutdown procedure may be necessary First flush the system with wash solvent for approximately 30 minutes at the appropriate flow rate for the column in use Second turn the pumps off and close the controller software Turn the solvent select valves to the upward Off position so that no solvents are selected Power off the instrument and PC If Helium is still being supplied to the instrument wash solvent will continue to be flushed through the pump head pistons If the instrument is to be shut down for an indefinite period of time or to be moved then the system should also be depressurized and the Helium source shut off To depressurize the system first turn the Helium Valve to Off and choose Sparge Vent on the Helium panel Unscrew the Delrin plug on the cap insert of the wash bottle to allow the gas to escape Retighten the Delrin plug once the gas has escaped IV System Maintenance Proper maintenance of the instrument will help prolong the life of the MAGIC 2002 and its components Change solvents on a regular basis to help to keep contamination problems and or particulate formation from occurring Routinely check al
14. The Outlet check valve arrow should be pointing away from the pump head The Inlet check valve arrow should be pointing toward the pump head Reinstall the housing to the pump 37 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com head and the tubing to the housing Do not over tighten Once the new check valve is in place follow the procedure outlined on page 24 under Solvent Flow to the Pump Heads and then purge the pumps to remove air as described on page 26 under Purging Pumps to Remove Air Figure 27 Pump head Outlet check valve housing Clear plastic sealing surface Clear plastic sealing surface Inlet check valve housing Replacing Pump heads Prior to replacing a pump head stop all pump flows and solvent flows close EZChrom Elite software and turn the power to the instrument off Remove the tubing Refer to Figure 28 page 39 by using a 4 open ended wrench and then remove the pump head by unscrewing the four Allen screws To install the new pump head simply reattach the tubing and Allen screws Once the new pump head is installed turn the power to the instrument back on and allow the detector to re initialize prior to re opening the EZChrom Elite software Verify solvent flow to the pump heads by following the procedure outlined on page 24 under Solvent Flow to the Pump heads and then purge the pumps to remove air as outlined on page 26 under
15. control used to change flush out and re equilibrate the system prior to restarting a run 33 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 23 Instrument Status of Temperature Controller Screen Displays the MAGIC column Updates all fields with current When the Status Enabled is not heater temperature when Status instrument operating conditions checked clicking on this button Enabled is checked Allows for sends the value in the Temperature entry of temperature when C field to the MAGIC effective Status Enabled is not checked immediately Instrument Stats Figure 24 Instrument Status of Pumps Screen Updates all fields with current Updates the instrument with Displays or sets the Displays or sets instrument operating conditions the values in all of the fields total system flow rate the B Displays the Instrument Status Al Displays installed pump or sets the heads when Status Injector Enabled is checked valve Note The first position time the software is SEP iY YO Y a lt Also launched you must A AE shows set Status Enabled which before you can ports are send data to the I connected pumps Ti in each J position Displays the status of the pump flow when Status Enabled is checked Allows starting and When used with the Manual Set button Displays the method run Displays total stopping
16. flow when allows purging of the pumps to remove time when Status Enabled system pressure used with the air bubbles Note You must Manual is checked when Status Manual Set button Set to start and stop purging Enabled is checked 34 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 25 Instrument Status of External Events Screen Updates all fields with current Updates the instrument with instrument operating conditions the values in all of the fields hw Instrument Status AGIC Temp Controller MAGIC Pump Extemal Events MAGIC Detector Status Enabled Manual Set Event MAGIC High Flow A MAGIC High Flow B Load Inject Output A Output B Output C NO400 517 9 Displays the status of the events in real time when the Status Putting a check mark in the On and Enabled is checked Displays if the pumps are in High Flow or Change boxes and clicking on Manual Low Flow modes Can be used with the Change box to set events Set will cause that event to happen Figure 26 Instrument Status of Detector Screen Updates all fields with current Updates the instrument with Allows manual auto zero of Displays instrument operating conditions the values in all of the fields the detector when used with lamp when checked the Manual Set button status or allows I Manual Displays the h Instrument Status iol x Set to turn current number a
17. position directly opposite the R setting If no test certificate is available a good starting point is R3 with a flow rate of 100 l min Confirm flow through the column prior to connecting it to the flow cell inlet tube Once the system pressure has stabilized check the flow cell outlet tube to ensure some liquid is flowing through the column and flow cell If no measurable flow is obtained in 15 20 minutes there may be a leak or blockage in the system Check the fittings around the injector from the column to the flow cell and the flow cell outlet fittings Measuring and Adjusting Flows Measure the flow rate as described on page 37 under Measuring Flow Rates and adjust the pump flow rate and or the restriction as needed to achieve the desired capillary flow rate When it is necessary to switch from one splitter level to another turn pump flow off and allow the column to depressurize to less than 100 PSI If the measured flow rate is more than the desired capillary flow rate simply decrease the pump flow rate If it is necessary to decrease the pump flow significantly below 50 ul min then it is best to move the tubing going into the Variable Splitter Box to an R value setting that provides less restriction This will allow the pumps to run under optimal conditions of 50 100 ul min yet deliver the appropriate flow through the column If the measured flow rate is less than the desired capillary flow rate move the tubing going into
18. right hand side of the MAGIC 2002 The compartment door is hinged on the bottom Refer to Figure 9 page 21 so that the top of the door moves out and down to allow access to the column UV detector photodiode and flow cell assembly The opening of this door causes the temperature controller to shut off The compartment is designed to handle various internal diameter columns and column lengths up to 250 mm Conventional columns are connected to the flow cell assembly using a Michrom BioResources Inc Standard Column Adapter Michrom BioResources Inc columns are connected directly into the bottom of the flow cell assembly The flow cell assembly Refer to Figure 10 page 21 consists of an outer diode secured to the mounting plate with two thumb screws mounting plate flow cell holder flow cell and flow cell outlet line The flow cell is held in the holder by the outlet line and a locking nut An analytical system consists of a 5 mm path length flow cell 2 4 ul volume with a 0 007 inch ID outlet line 5 ul volume The microbore assembly consists of a 2 mm path length flow cell 0 3 ul volume with a 0 003 inch ID output line 1 ul volume A capillary assembly consists of a 2 mm path length flow cell 0 02 ul volume with a 0 002 inch ID outlet line 0 6ul volume The flow cell output line is attached to the waste line via the stainless steel bulkhead union located on the ceiling outside the column compartment Alternatively the flow cell
19. tube A standard column adapter P N 602 25026 01 should be used with all non Michrom BioResources Inc columns that use conventional 10 32 end fittings The column inlet line should be connected to the column inlet or guard column inlet or be directed to a waste reservoir prior to pressurizing the MAGIC 2002 solvents with Helium Helium head pressure 6 PSI will cause solvent flow with no system back pressure if the solvent select valves are in the A B or wash postions Note It is recommended that all pump flows be turned off whenever installing or changing a column Solvent Sparging and Detection of Helium Leaks Confirm that all solvent containers have the appropriate solvents and are properly sealed The solvent bottle cap inserts form a seal between the Viton o ring and the bottle tops when pressurized with Helium Inspect the tops of the bottles for chips and or cracks and ensure that each o ring is properly seated in the groove on 23 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com the underside of the cap insert prior to tightening the caps on the bottles The system is shipped with plastic coated bottles with plastic pouring rings removed from the top If other bottles are to be used with the MAGIC 2002 verify they are clean and crack chip free before use Although the Viton o rings are very inert some organic solvents can cause the o rings to swell in size If the o ring
20. 5420 z y Board Number 1 us nau Analog Input Chan A Enter mAU for the Y Avis Multiplier f1e 004 Axis Units Cancel Enter 1e 003 for the Click on OK after Y Axis Multiplier entering all of the data 60 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 48 Detector Configuration Screen UV 2 Enter UV 2 for the Detector Name Detector Configuration Select Analog for the Detector Name fuv2 Detector Model DetectorModet nao j Click on this symbol Acquisition Source 55420 H and select l Board Number 1 Select SS420 for the Mau Pa ooo Analog Input Chan B Acquisition Source YAxisMuliplie e003 OOOO Cancel Help Enter mAU for the Y Axis Units Enter 1e 003 for the Click on OK after Y Axis Multiplier entering all of the data Figure 49 Detector Configuration Screen Fraction Collector Enter Fraction Collector for the Detector Name Select Analog for the Detector Model Detector Configuration Detector Name Fraction Collector E T umama ae a ie symbol Select SS420 for the Acquisition Source 55420 z 8 Board Nanas 1 Acquisition Source rr Asi Units frau Analog Input Chan C Axis Multiplier fi e004 Enter mAU for the Y Axis Units Click on OK after Enter 1e 003 for the entering all of the data Y axis Multiplier 61 Artisan Technology
21. 66 The following table lists the Events by Name the Source and the Setup for each event Table 4 Control Only Event Configuration Name Source Setup Board Input Triggered State Trigger CIO DIO 0 BO Closed Ready CIO DIO 0 A4 Closed MAGIC High Flow A CIO DIO 0 AO Closed MAGIC High Flow B CIO DIO 0 Al Closed Load must be typed in CIO DIO 0 A2 Inject must be typed in CIO DIO 0 A3 MAGIC Get Load CIO DIO 0 Bl Closed MAGIC Get Inject CIO DIO 0 B2 Closed Output A must be typed in CIO DIO 0 A5 Output B must be typed in CIO DIO 0 A6 Output C must be typed in CIO DIO 0 A7 Figure 59 MAGIC Pump Configuration Screen Select COM 3 for Pump A Com Port Select COM 4 for Pump B Com Port Select the Pressure Units Choices are PSI Mpa or Kg M2 Enter the maximum operating pressure The pumps will shut off if the pressure goes above this value MAGIC Pump Configuration After entering all of the data click on OK Enter the minimum operating pressure The pumps will shut off if the pressure drops below this value Pump Com Port Pump B Com Port W essure Units Max pressure in PSI Min pressure in PSI LOAD Event coms zj Entering a value of COM4 Ej Cancel 0 disables this function PSI 7 Help EB fo Select the LOAD INJECT Event Inject B T l 67 Event This should always be set to Load S
22. 88 88 SOURCE www artisantg com CHAPTER 2 BASIC OPERATION Initial installation of the MAGIC 2002 is normally performed by Michrom BioResources Inc personnel or their authorized agents Instrument installation includes power cabling Helium pump heads flow cell column solvent bottles and accessories The gas and solvent connections system controller data system and accessory connections are made on site and system integrity is verified If the MAGIC 2002 system is removed or shipped to a new location note the cabling configuration for proper reinstallation If problems arise after moving the MAGIC 2002 contact Michrom BioResources Inc or the authorized representative in your area for technical support Note The MAGIC 2002 is shipped with two lock down screws for each pump fastened to the back of the instrument These screws are normally removed during installation to allow access to the pump compartments from the front of the instrument Note Although the system can be moved without these lock down screws in place it should not be shipped without replacing these lock down screws I System Startup Column Installation Michrom BioResources Inc s microbore columns 0 5 1 0 and 2 0 mm ID are designed to thread directly into the Flow Cell Holder refer to Figure 9 page 21 Michrom BioResources Inc s capillary columns 0 1 0 2 and 0 3 mm ID are designed to thread into a specially designed Flow Cell Inlet
23. Plug Upchurch Lite Touch 10 32 Nut Upchurch Lite Touch Ferrule Front and Rear Tubing Peek 007 Tubing Peek 010 Tubing Peek 030 10 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com CHAPTER 1 INSTRUMENT DESCRIPTION I Overview The MAGIC 2002 is the first of a new generation of HPLC systems designed to let the user translate methods from analytical to microbore levels with no impact on the separation The MAGIC 2002 s dual pump high pressure binary gradient solvent delivery system will deliver accurate gradient flows from 0 5ul min to 5 0 ml min The system will accept a wide range of column sizes from 50um to 10mm ID and from 50mm to 250mm in length Conventional analytical columns or Michrom BioResources Inc s custom microbore columns or capillary columns can be utilized The built in dual wavelength UV Vis detector has flow cells to cover the entire gradient flow range Auxiliary detectors including fluorescence electrochemical radioactivity or mass spectrometer can be added with no impact on extra column band broadening The MAGIC 2002 design allows for the connection of peripheral devices and accessory items that automate sampling fraction collection and online sample preparation Figure 1 MAGIC 2002 with PC 11 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com II Basic Component
24. Vis Enter the total run time for the method Figure 19 Instrument Setup of Trigger Screen Select the type of trigger that you want to start the method Choices are None Manual and External Use External when you want another device like an autosampler to start the method Instrument Setup 30 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Single Run A single run can be performed by clicking on the dark blue single arrow on the tool bar Refer to Figure 13 page 26 This will bring up the Single Run Acquisition Screen Refer to Figure 20 Figure 20 Single Run Acquisition Screen Single Run Acquisition To verify that the system is operating properly analyze a standard using the appropriate flows for the column of choice Refer to Chapter 3 Section I page 41 If a 1 0mm ID column is in line simply use the method as created in figures 14 19 Prior to starting a run flush the sample loop with A Solvent to ensure that the loop is free of organic solvent greater than 5 Load a syringe with the appropriate amount of test mixture and then load the syringe contents into the sample loop Refer to Chapter 3 Section I for column capacity For example If a 1 0mm ID peptide column is in line load the syringe with 5ul of A Solvent and 10ul of the 10ng ul Synthetic Peptide Test Mix Part Number 910 00002 02 and then load the loop with the syringe
25. annel 4 Uw SN 1085 Parabens Ez Instrument Status MAGIC Temp Controller MAGIC Pump Extemal Events MAGIC Detector I Status Enabled Manual Set Temperature C fo For Help press F1 Purging Pumps to Remove Air The pump purge flow rate for each pump is 1 ml min for microbore pump heads and 5 ml min for analytical pump heads The pumps are purged to remove air in the solvent lines and to replace wash solvent with gradient solvents to the column inlet The minimum purge time required to displace system solvents and or air bubbles is approximately 30 seconds With solvent flow verified to the pump heads Refer to page 24 Solvent Flow to the Pump heads close the purge valves ensure solvent select valves are set to the wash solvent position and direct the column inlet line to a waste container The system purge control is found on the Magic Pump tab in the Control Instrument Status window Refer to Figure 24 page 34 Disable the Status Enabled if checked and click on the Pumps On Purge On for both pumps A and B then click on Manual Set A warning box will be displayed for each pump to open purge valves as a precautionary reminder in case the column is in the solvent pathway Opening the purge valves is not required if the column inlet line is removed from the column prior to purging With both pumps purging at 1 0 ml min pressure should build to between 300 600 PSI and a continuous stream of solvent should be e
26. ber com The Michrom BioResources Inc staff thanks you for your interest in our product line and look forward to serving you in the future 69 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com A rtisan Artisan Technology Group is your source for quality TecmoogyGroup new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment 7 EQUIPMENT DEMOS HUNDREDS OF InstraV ea REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED s Z tia Contact us 888 88 SOURCE sales artisantg com www artisantg com
27. best sensitivity As shown in Table 2 the HPLC column diameter should be matched to the capacity of analytes retained components of the injected sample to insure optimum chromatography Too large a sample load may cause excessive band spreading saturation of detection and or contamination of the column However too small a sample load may cause excessive analyte dilution limited detection signal to noise and or nonlinear recovery due to excess adsorptive surfaces Sample injection volume can also be a rate limiting step since sample volumes greater than 10 of the column volume may contribute to band broadening unless specialized loading techniques are employed Table 2 HPLC Column Capacity amp Flow Conditions Column 5cm Analyte Optimum Flow Rate Optimum ID Bed Vol Capacity Flow Rate Range MAGIC W ul grams ul min ul min Flow Range Kit 50 0 1 lt 10 0 10 0 05 0 50 Capillary 100 0 4 10 10 0 50 0 25 2 50 Capillary 200 1 6 10 10 2 00 1 00 10 0 Capillary 300 3 6 10 10 5 00 2 00 20 0 Capillary 500 10 10 10 12 0 5 00 50 0 Microbore 1000 40 10 10 50 0 20 0 200 Microbore 2000 160 10 10 200 100 1000 Microbore 3000 360 10 10 500 200 2000 Analytical 4600 800 10 107 1000 500 5000 Analytical 41 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com With the analytical or microbore Flow Range Kits installed on the MAGIC 2002 no s
28. bing diagram Figure 32 for the standard setup Figure 31 Auto Sampler Wiring Autosampler Wiring EXT INJECT INPUT EXT START OUT A B c par Autosampler MAGIC GND EXT INJECT INPUT NO normally open COM Figure 32 Auto Sampler Plumbing HPLC Autosampler Plumbing inject 46 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Operating with a Michrom BioResources Inc Auto sampler Whenever an auto sampler will be used to trigger the MAGIC 2002 it is necessary to set the trigger in the control software to External Program the auto sampler to run according to the desired parameters with the correct number of injections IV Trapping Options Michrom BioResources Inc has designed trap cartridges that allow automated sample preparation concentration desalting buffer exchange and detergent removal prior to analysis by the MAGIC 20021 Configuration on the MAGIC The traps are designed along with a trap holder to take the place of the sample loop port 10 and port 3 on the injection valve Using a Trap Cartridge To achieve the best results using a trap some sample handling requirements are necessary The sample should be loaded in the solution which ensures solubility of all the components of interest Solvent A yet has very low chromatographic mobility The trap should be washed with a solution similar to the sample loading
29. chnology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 22 Run Sequence Screen Run Sequence 9 9 e Instrument Status The Instrument Status window is where direct control of each of the MAGIC 2002 components can be performed Manually setting individual parameters can also be performed as well as monitoring the status of the current operating conditions during a single method or sequence run To open the Instrument Status window click on Control Refer to Figure 13 page 26 and select Instrument Status Each component of the MAGIC 2002 system has a separate tab Refer to Figures 23 26 page 34 35 in which to enter data and or monitor status If no check mark is present in the Status Enabled box data can be entered into any white field When the Manual Set button is clicked all of the values in the fields are sent to the instrument and take effect immediately When there is a check mark in the Status Enabled box the fields reflect the current instrument conditions The Status Enabled box should always be checked during a single method or sequence run Although the Manual Set function can be used to change conditions manually during a run these changes will not be saved with the method or data file making it difficult to interpret results at a later time If a method run is not going as expected no peaks or sample elutes at time zero the method should be stopped and Manual
30. contents Once the sample loop has the appropriate contents start the run An example of a typical test chromatogram on 31 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 100 a 1 0mm ID peptide column Part Number 900 61211 00 is provided below Refer to Figure 21 Figure 21 Michrom BioResources Inc Peptide Standard Chromatogram J 100 T T T T T T T T T T T 0 0 2 5 5 0 7 5 10 0 12 5 15 0 17 5 20 0 22 5 25 0 27 5 30 0 Minutes Column Type __ MAGIC MS C18 Size 5u 200A Part _ 900 61211 00 Dimensions _ 1 0 X 150mm Gradient 5 B 65 B in 20 min Solvent A 2 98 0 1 ACN H20 TFA Solvent B 90 10 0 09 ACN H2O TFA Flow Rate 50ul min Test Mix _ Part 910 00002 02 Synthetic Peptide Test Mix Sequences Sequences link individual methods together for multiple automated runs Sequences can be created method by method or by multiple runs of the same method To create a sequence click on File Refer to Figure 13 page 26 choose Sequence then choose New Sequence and follow the Sequence Wizard Save the sequence once the wizard is finished To run a sequence click on the green double arrows button on the tool bar Refer to Figure 13 page 26 This will pull up the Run Sequence Screen Refer to Figure 22 page 33 Please refer to the EZChrom on line manual on the CD ROM for details 32 Artisan Te
31. d prior to running it in the single run or sequence run mode Methods will be saved automatically with a met extension unless otherwise specified 27 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 14 Instrument Setup of Temperature Controller Screen Enter the desired temperature for the method Acceptable range is from 20 to 60 C Instrument Setup Figure 15 Instrument Setup of Pumps Screen Select the type of Enter the gradient information for the method run pump head installed on the Instrument Setup D xj MAGIC Select which pump or pumps that you want flowing in the method Enter total run time for the method Select the injector valve position This is the position the injector will be in while the method waits for a trigger Selects between table and graphic display of the gradient information Must select table to edit the gradient 28 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 16 Instrument Setup of External Events Screen Select the active state of the output Choices are Open or Closed Enter the amount of time that you want the output to be active Enter the time that you want the output to occur Select the output that you want to occur during the method run
32. e into the sample loop of the instrument be sure the sample is in solvent which is less than 5 organic in composition The D lamp has expired Replace the D gt lamp with a new one The active procedure does not specify the lamp being ignited and a wavelength selected Verify that the detector is turned on and a wavelength selected in the active procedure The programmed gradient does not call for sufficient B organic solvent to be delivered to enhance peak elution Program the gradient to deliver at least 65 B solvent 90 organic to sufficiently enhance peak elution 53 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com SYMPTOM POSSIBLE CAUSE SOLUTION No peaks are observed when a sample is run Continued The sample loop contains a high percentage of organic solvent This has caused the peaks to elute in the solvent front Flush the sample loop with a low percentage of organic solvent 2 with the valve in the load position Irreproducible retention times There is a leak at a fitting on a pump head purge valve or the 10 port valve Shut flow off to pumps via the control software Turn solvent selection valves to the vertical or Off position Remove the fitting and thoroughly dry ferrule and ferrule seat using a Kimwipe Pump head check valve s failed Replace check valve s w
33. el Pump skid Face Plate Injection Valve Panel Column Compartment Opened Flow Cell Assembly with Photodiode Instrument Setup of Temperature Controller Screen Instrument Setup of External Events Screen Instrument Status of Pumps Screen Fraction Collector Wiring Step 2 SS420 Configuration Screen EZChrom Elite Client Server Screen UV Vis Lamps Compartment Opened EZChrom Elite Client Server Screen Instrument Status Preview Screen Instrument Setup of Data Acquisition Channels Screen Instrument Setup of Detector Screen Instrument Setup of Trigger Screen Instrument Status of Temperature Controller Screen Instrument Status of External Events Screen Instrument Status of Detector Screen Auto sampler Plumbing Diagram Fraction Collector Wiring Step 1 EZChrom Elite Client Server Screen Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figures Continued Page 45 MAGIC Detector Configuration Screen I a 60 46 MAGIC Temperature Controller Configuration Screen a 60 47 Detector Configuration Screen UV 1 I 1 60 48 Detector Configuration Screen UV 2 II I 61 49 Detector Configuration Screen Fraction Collector U a 61 50 Detector Configuration Screen Pressure UU I a a a 62 51 EZChrom Elite Client Server Screen a 63 52 Interface Configuration Screen 63 53 CIO DIO Configuration Screen n ur rr aaa a 64 54 EZChrom Elite Cl
34. elect the INJECT Event This should always be set to Iniect Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 60 MAGIC Detector Configuration Screen Select COM 5 for Click on OK after selecting the Detector Com the Detector Com Port Port MAGIC Detector Configuration Detector Com Port COM 5 z Figure 61 MAGIC Temperature Controller Configuration Screen Select COM 6 for the Click on OK after select the Temperature Controller Temperature Controller Com Com Port Port Controller Com Port Jcom 6 z After all four modules have been configured Click OK on the MAGIC Configuration screen to save these configurations and return to the MAGIC EZChrom Elite Client Server screen Double click on the MAGIC 2002 Icon to open the MAGIC software 68 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com The contents of this operator s manual were compiled with the intent to provide the instrument user with all of the information necessary to operate the MAGIC 2002 HPLC and the associated peripheral devices and accessories available from Michrom BioResources Inc Further clarification and or additional information can be obtained from the Michrom BioResources Inc technical support staff at the following numbers Telephone 530 888 6498 Fax 530 888 8295 e mail michromser psy
35. en ports 5 and 8 on the injection valve Connect the column inlet tube to port 9 on the injection valve 10 11 Close the controller software and power the unit off Install the Capillary Flow Cell Assembly in place of the Microbore Flow Cell Assembly Turn the power to the instrument back on wait 2 3 minutes for detector to reinitialize and then restart the controller software Checking for Leaks After installing a column check the new setup for leaks To do this turn the solvent select valves on and start the pumps flowing at 100 uL min at 50 B Check all fittings around the injection valve the mixer and the MAGIC Variable Splitter for leaks and confirm flow through the column inlet tube Column Installation With the capillary system it is especially important to minimize the introduction of air to the flow cell Once flow through the column inlet tube is confirmed allow the system to run for approximately 3 5 minutes Turn the pump flow off and dry the end of the tube Connect the column to the column inlet tube Refer to the Column Test Certificate for an approximation of flow parameters to achieve the desired flow rate Choose the MAGIC Variable Splitter level and set the flow rate in the control software according to the test certificate Change the 44 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com waste line on the MAGIC Variable Splitter to the W
36. ent bottle for wash solvent 40 40 20 ACN ProH H 0 Main Power Switch Contains power entrance block main fuses power switch and voltage select switch Flow Cell Outlet Access Port Provides accessibility to flow cell outlet for flow measurements or connection to peripheral devices mass spectrometer fraction collector etc Injection Valve Access Port Provides accessibility to injection valve for auto sampler or other in line devices 14 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com HI Hardware Description This section provides the user with a better understanding of the MAGIC 2002 HPLC system and its components Solvent Degas Pressurization System The Helium input line is located on the rear instrument panel Refer to Figure 3 page 13 There is an additional Helium output for attachment of accessory devices such as the Michrom BioResources Inc Pulseless Flow Module The solvent degas system internally regulates pressure to 6 PSI The system gas is split to independently degas and pressurize the A B solvent bottles and to pressurize the wash solvent bottle Solvent Bottle Degas Pressurization System There are two switching valves above the solvent bottles on the inside front door of the pump compartment Refer to Figure 5 page 16 The first valve is a Helium shutoff valve and is used to introduce or interrupt Helium flow to the A B solvent bottles Please n
37. ent is turned off Proper precautions should be taken when working with electronic components in the instrument Never try to repair or replace an instrument component not described in this manual without first consulting Michrom BioResources Inc or its authorized representative in your area Good Laboratory Practices Maintaining Records Michrom BioResources Inc recommends that records of system operating conditions be maintained At a minimum keep a chromatogram of a standard test mixture along with the associated system conditions Careful comparison of retention times peak shapes column pressure peak sensitivity and baseline noise can provide valuable clues to identifying and solving problems Chemical Toxicity The large volume of toxic and flammable solvents used and stored in laboratories can be potentially dangerous In addition the potential hazards posed by samples should be recognized Special care should be taken to follow all precautions necessary to ensure proper ventilation storage handling and disposal of both solvents and samples Become familiar with the toxicity data and potential hazards associated with all chemicals by referring to the manufacturers Material Safety Data Sheets MSDS Sample Preparation Filter samples that might contain particulate matter using a 0 45um filter Always consider the solubility of your sample in the mobile phase Sample precipitation can plug the system by obstructing the flow th
38. h pump head top and bottom are the wash solvent lines for solvent flow behind the pump head piston seals The inlet solvent line from the solvent select valve is located on the lower left side of the pump head An inlet check valve is the interface between the inlet line and the pump head The outlet check valve is located on the upper left side of the pump head and is the solvent path to the lower right side of the pump head Solvent exits the pump head from the upper right side and is directed into the bottom of the purge valve via a stainless steel tube The purge valve Refer to Figure 7 houses the pressure transducer There are three solvent lines attached to the purge valve The stainless steel inlet line from the pump head is located on the bottom of the purge valve The PEEK line to the mixer is located on the top of the purge valve The Teflon line on the right side of the purge valve is routed to the waste container The purge valve in the closed position completely clockwise until finger tight allows solvent to flow through the PEEK line exiting the top of the purge valve to the mixer The volume from the purge valve to the mixer is approximately 65ul The purge valve turned counter clockwise to the open position allows for the venting of solvent and air to waste via the Teflon line exiting the purge valve to the left Further discussion on displacing air bubbles from the pumps using the purge valves is found in Chapter 18 Artisan Tec
39. hnology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 2 Section I under System Startup It is not necessary to fully open the valve multiple full revolutions of the valve during purging Variable Volume Mixer Solvent passes from the pumps to the dual stage variable volume mixer Refer to Figure 8 page 20 located below the column oven compartment adjacent to the 10 port injector valve The first stage is a static vortex mixing tee that is followed by a high pressure static mixer cartridge The cartridges are designed to provide effective gradient delay volumes with effective mixing to cover the gradient range of the MAGIC 2002 Refer to Table 1 The housing for the mixer cartridge is mounted onto the valve actuator panel of the instrument for easy accessibility Table 1 MAGIC Mixer Cartridges Cartridge Size Physical Total Sweep Out Volume Effective Flow Rate Volume Range 50 ul 18 ul 50ul 20 100u1 min 100 ul 36 ul 100u1 40 200u1 min 200 ul 72 ul 200u1 100 500p1 min 500 ul 180 ul 500u1 500 2500u1 min 1000 ul 360 ul 1000u1 500 5000u1 min To reduce baseline noise and maximize reproducibility for slow lt 1 min high sensitivity gradient runs use a larger mixer volume For fast gt 1 min gradients and faster system re equilibration use a smaller mixer volume Injector Valve The flow input from the mixer routes to the 10 port injector va
40. ient Server Screen eee 64 55 EZChrom Elite Client Server Sereen a 65 56 Instrument Configuration Screen ur L 65 57 MAGIC Configuration Screen ur Iurraea lt TSsCUL a 66 58 Event Configuration Screen 66 59 MAGIC Pump Configuration Screen u a a 67 60 MAGIC Detector Configuration Screen 68 61 MAGIC Temperature Controller Configuration Screen 1 1 68 Tables Page l Miker Cartridees nn ae s Fa ae alate asua a ul uuu 19 2 Flow Rate Optimization by Column Dimension sss 41 3 Data Acquisition Event Configuration II aaa 59 4 MAGIC Control Only Event Configuration 67 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com WARRANTY amp SERVICE AGREEMENT MAGIC Warranty Policy Michrom BioResources Inc the Company warrants the Micro Analytical Gradient Integrated Chromatograph 2002 MAGIC 2002 for a period of one year from the date of delivery by the Company to the original buyer against defects in materials and workmanship under normal use and maintenance as described in the operating instructions within this manual This warranty covers all of the MAGIC 2002 components excluding expendable items such as columns check valves pistons piston seals lamps trap cartridges rotors purge valves bottles and tubing The exclusive remedy for defects in material and workmanship at the option of Michrom BioResources Inc shall be replacing or repairing the
41. ist of Spare Parts r sua Ia aa uu 10 Chapter 1 Instrument Description ssi ua aaa 11 L Overview ee ee DDD eee tne A L 11 II Basic Components I ur e aaa a 12 III Hardware Description ss a uuu a 15 Chapter 2 Basic Operation sr sraoe u aaa 23 I System Startup rr ur ia aa 23 IL Operating the MAGIC20027M I aa 27 III System Shutdown 36 IV System Maintenance 36 V Re Instalinga MAGIC 20072 I T a 39 Chapter 3 Options L 41 L Selecting Flow Range Kits for Optimum HPLC Conditions _ 41 II Capillary Flows I uurra usura u 42 III Autosampler a ur usr aaa aaa aaa 45 IV Trapping Options rd ura XeLl aaa 47 V FBraction Collector bd 47 AppendiX rr 51 I Troubleshooting Guide 51 II Configuring the MAGIC 2002 for use with Data Acquisition _ 55 Il Configuring the MAGIC 2002 for Control only 63 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figures OS Oye YN BRR RRWWWWWWWHWWWNNNNNNNNNNE EPSP ee eee KRONE SODBNIANUEREWDNESVBDIAANEWNHKE SCOMINIDNKRWNKE SO Instrument Setup of Pumps Screen Run Sequence Screen Fraction Collector Wiring Step 3 EZChrom Elite Client Server Screen Interface Configuration Screen Interface Configuration Screen MAGIC Configuration Screen Event Configuration Screen MAGIC Pump Configuration Screen MAGIC 2002 with PC Right Side Panel Helium Panel Solvent Select Panel amp UV Vis Lamps Pan
42. ith new or rebuilt check valve s Pump head seals are worn or scored Run pumps isocratically in Wash solvent at 50 ul min 5 pump B for 30 minutes Save pressure data Now run pumps isocratically in Wash solvent at 50 ul min 95 pump B for 30 minutes Save pressure data Fax data to Michrom BioResources Inc for diagnosis Run pumps isocratically in Wash solvent at 50 ul min 5 pump B for 30 minutes Save pressure data Now run pumps isocratically in Wash solvent at 50 wl min 95 pump B for 30 minutes Save pressure data Fax data to Michrom BioResources Inc for diagnosis by technical support personnel Air bubble trapped in pump head Disconnect column inlet tubing Set the pumps on purge via the software and open and close both purge valves on pumps to release trapped air bubbles 54 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Il Configuring the MAGIC 2002 Instrument for use with Data Acquisition After installation of the EZChrom Elite and MAGIC 2002 software it is necessary to configure the computer hardware To configure the MAGIC 2002 hardware first open EZChrom Elite by clicking on Start going to Programs going to Chromatography and selecting EZChrom Elite Figures 36 through 50 present step by step instructions for configuring the computer hardware for instrument control and data acquisition Figure 36
43. l solvent levels and fill the reservoirs as needed so as not to run the system dry Follow the proper shutdown procedure to help prolong the life of the pump heads and detector lamps 36 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Solvent Replacement Whenever replacing solvents the system will need to be depressurized prior to opening the bottles First turn all pump flows off and place the solvent select valves in the upward Off position Turn the Helium to the Off position on the Helium panel and the Sparge Vent valve to Sparge Vent Turn the main Helium supply off and then vent the wash bottle by loosening the Delrin plug and allowing the gas to escape The solvents can be replaced once the system has been completely depressurized Once the solvents are replaced then the system should be re pressurized and checked for Helium leaks as discussed on page 22 under Solvent Sparging and Detection of Helium leaks Measuring Flow Rates Flow measurement readings should be taken with the column in line for consistent back pressure Flow measurements are taken from the flow cell outlet tube for accurate readings To take a flow measurement the following items are needed a union Part Number 004 25027 00 the manual injection port from port 1 of the injection valve and a syringe with appropriate volume to see 2 5 minutes of flow Prior to taking flow measurements allow the
44. lve Refer to Figure 8 page 20 This valve is for sample loading and provides additional ports for flexibility in sample handling The standard loop volume for microbore flows is 20 ul although loops ranging from 0 5 1000ul are available Michrom BioResources Inc trap cartridges for concentrating and desalting samples see Trapping Options in Chapter 3 can also be used as sample loops There is an electronic switch on the left side of the valve for manual load inject positioning of the valve The injector sensing positioning is also controlled from the software via both Control Instrument Status and Method Instrument Setup menus The injector position and port connections can also be observed on the Control Instrument Status screen Samples can be manually loaded with a syringe through the manual injection port located on valve port number one Valve port one is located on the top of the valve and is accessible with the door closed The volume from the mixer to the end of the column inlet line with a 20 ul loop is approximately 40 ul 19 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 8 Injection Valve Panel Manual Injection Manual Switch Injection Port 10 Port Injection Valve Column Compartment and Flow Cell The column compartment is a temperature controlled forced air heated oven The column oven compartment door is located in the top portion on the front
45. mp B Solvents are delivered to the pump heads from either the solvent bottles on the door or the wash solvent on the right side instrument panel The solvent from the A bottle travels through the left side solvent select valve Refer to Figure 6 into the upper pump head Solvent from the B bottle travels through the right side solvent select valve into the lower pump head If the solvent select valves are positioned inward toward Wash then the wash solvent travels through the pump heads If the solvent select valves are in the upward or Off position no solvent is going to the pump head The solvent select valves interrupt solvent flow to the pump heads that occurs due to Helium head pressure on the solvents Positioning the valves in the Off upward position allows the operator to change the column injector loops mixer or pump heads without depressurizing the A B Solvent bottles Never turn the solvent select valves to the upward position if the pumps are running Operating the pumps without solvent supplied to the pump heads may cause permanent damage to the pump head seals and pistons Figure 6 Solvent Select Panel amp UV Vis Panel Solvent UV Vis Lamp Selection Panel Compartment A Pump B Pump Upper Pump Lower Pump Solvent Solvent Selection Valve Selection Valve The pump status indicators are on the left portion of each pump faceplate Refer to Figure 7 page 18 The power indicator LED light emi
46. nch brass Swagelok nut and ferrule and 1 8 inch PEEK tubing Refer to Figure 3 page 13 The other end of the tubing is connected to a Helium source regulator set between 10 and 200 PSI DO NOT TURN HELIUM ON TO THE INSTRUMENT AT THIS TIME Install solvent bottles A and B filled with the appropriate solvents in the front left door of the instrument Refer to Figure 2 page 12 Install the wash solvent bottle filled with the appropriate solvent in the waste compartment located in the rear of the right side panel of the instrument Refer to Figure 4 page 14 Turn the Helium On Off valve located on the inside of the left front door to the Off position Refer to Figure 5 page 16 Turn the Pressurize Sparge Vent valve to the Pressurize position Set Helium flow to the MAGIC 2002 by opening the valve on the Helium source Turn the Helium On Off valve to the On position Refer to Figure 5 page 16 The filters in the bottom of the solvent bottles will begin to bubble sparge Helium into the solvents This sparging will continue until the bottles are fully pressurized Power The MAGIC 2002 can accept voltages of 100 120V AC and 220 240V AC The power entrance to the MAGIC 2002 is located at the rear of the right side panel Refer to Figure 4 page 14 This is where the power cord to the instrument is inserted and where the voltage selection switch is located The MAGIC 2002 is shipped with 115V AC as the default voltage selection
47. nnector PC I O The connection between the Data System PC board or the I O PC board and the MAGIC 2002 15 pin I O connector Remote I O Remote I Os that are programmable in a Procedure A B C 1 e Fraction Collector start and external input and output for auto sampler interfacing PC RS 232 Connection between the PC RS 232 board and the MAGIC 2002 78 pin UV Outputs The connections between MAGIC 2002 and external devices to collect UV data Pressure Outputs The connections between the MAGIC 2002 and external devices to collect pressure information Helium Connections The location for connection of Helium to the MAGIC 2002 The recommended input is 20 200 PSI 13 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com DESCRIPTION OF RIGHT SIDE PANEL Figure 4 Right Side Panel Flow Cell Outlet Access Port Air Inlet for Forced Air Oven Waste Compartment Wash Bottle Waste Container Injection Valve Access Port Main Power Switch Waste Wash Compartment Compartment designed to house the waste container and wash solvent bottle Waste Container 2 liter Nalgene bottle Note All Solvent waste lines should go into the waste bottle and the bottle should be emptied when the waste solvent reaches the waste lines If not waste solvent may back flow out to the manual injection port Wash Solvent Bottle 1 liter solv
48. nt Server Screen Right mouse click on the new instrument icon and select Configure EZChrom Elite Client Server P The Enterprise Figure 56 Instrument Configuration Screen Type in the name of your instrument MAGIC 2002 Click on Configure Select MAGIC for the type of instrument JSI ria E 65 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 57 MAGIC Configuration Screen Double clicking on each icon on the Available Modules side will move them over to the Configured Modules Detector MAGIC Pump Event MAGIC Configuration Detector MAGIC Temperatu as shown in Figure 57 Configure the Double clicking on each icon on the Configured Modules side will open the configuration screen for each module MAGIC Pump MAGIC MAGIC Detector Temperatu Move the four Modules from the Available modules side to the Configured modules side Event Configuration first Refer to Figure 58 and Table 4 pages 66 67 and then continue on until all the modules have been properly configured as shown on the following pages Refer to Figures 59 61 pages 67 68 Figure 58 Event Configuration Screen The Event Configuration should look like this when all events are configured Even Conlin Hn Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com
49. nterface Configuration ys EZChrom Elite Client File Edit View BES g Ea i Interface Configuration v System Administration Mode Cur Sustem Administration Wizard ar Options Pare e Maren Se aE A gh a ig Displays the instrument interface configuration Figure 52 Interface Configuration Screen Highlight the CIO DIO icon and click on Properties Interface Configuration 5420 A D Fissi 63 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 53 CIO DIO Configuration Screen Place a check mark here to install the board Select CIODIO24 for the board CIO DIO Configuration Model Set the Base Address to 320 by clicking on the switches to arrange them as shown Set the Connector to 0 Click on Port B to change it from an Output to an Input Click on OK after entering all of the data After setting the Interface configuration the computer must be restarted for the new hardware settings to take effect When the computer restarts click on Start go to Programs go to Chromatography and select EZChrom Elite Figure 54 EZChrom Elite Client Server Screen Select New Instrument EZChrom Elite Client Server 64 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 55 EZChrom Elite Clie
50. ote that this shutoff valve does not stop Helium flow to the wash solvent bottle The second valve is the Pressurize Sparge valve In the sparge position this valve allows Helium to freely enter the solvents and remove Oxygen degas solvents Excess air and Helium are vented from the solvents and bottles When the Pressurize Sparge valve is in the pressurize position the bottles are sealed and placed under 6 PSI of head pressure on the solvents Prior to opening a bottle to replace solvent the Helium shutoff valve must be closed and the Pressurize Sparge valve turned to the Sparge position This action allows the bottles to vent and releases system pressure The Helium to the solvent bottles has two functions First it serves to degas solvents by removing air that can cause problems with pumping separation and detection in the HPLC Second it equalizes the pressure on the two solvents This pressure equalization provides the stability required for the reproducible solvent delivery system necessary to form gradients between solvents of varying viscosities 15 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 5 Helium Panel Sparge Vent Helium On Off Valve Valve Wash Bottle Pressurization System The delivery of Helium to the wash bottle is constant at 6 PSI There is no shutoff valve for this gas line When replacing the wash solvent depressurize the wash bottle prior
51. our local power supply Never run the instrument at more than 8 below the nominal line voltage The power cord must be inserted into a power outlet with a protective earth contact ground When using an extension cord make sure that it is also grounded Do not change the external or internal grounding connections Tampering with or disconnecting these connections could endanger you and or damage the instrument Never operate the instrument with the top cover removed except during troubleshooting procedures under the direction of a Michrom BioResources Inc service representative Do not turn the instrument on if you suspect that it has incurred electrical damage Disconnect the power cord and contact Michrom BioResources Inc or its authorized representative in your area Damage can result if the instrument is stored for prolonged periods of time under unfavorable conditions e g temperature extremes humidity etc Prior to prolonged storage or shipment the instrument should be thoroughly flushed with wash solvent 40 40 20 Acetonitrile n propanol H2O to remove salts buffers acids bases or other chemicals that could cause corrosion or precipitation in the system Always disconnect the power cord before attempting any type of electronic maintenance Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Capacitors inside the instrument may still be charged even if the instrum
52. outlet line can be routed to an auxiliary detector and or fraction collector by attachment through the access port in the upper right side using a zero dead volume union or tee See Spare Parts List page 10 20 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 9 Column Compartment Opened Flow cell Assembly with Flow cell Outlet Photodiode al Tube Column Inlet Tube Figure 10 Flow Cell Assembly with Photodiode Outlet Tube Photodiode Flow Cell Flow Cell Holder 21 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com UV Vis Detector The UV Vis detector is located above the upper pump A solvent pump in the pump compartment Removing the UV VIS compartment front cover allows access to the UV lamp compartment Refer to Figure 11 The UV Vis lamp compartment houses the pre aligned Deuterium D2 lamp UV and Tungsten lamp Vis The MAGIC 2002 operates in either single or dual wavelength modes The lamp compartment cover must be installed for the lamp to operate If the cover is removed the lamp will turn off The lamp will restart re fire and the wavelength will reset when the door is reinstalled Figure 11 UV Vis Lamps Compartment Opened D Lamp UV Tungston Lamp Vis D Lamp Hour Meter 22 Artisan Technology Group Quality Instrumentation Guaranteed 8
53. parts determined to be defective This warranty is void in the event the MAGIC 2002 is altered or modified other than by or at the direction of Michrom BioResources Inc The foregoing warranty is exclusive and all other warranties whether expressed or implied including any warranties of merchantability and warranties of fitness for purpose but without limitation thereto are expressly excluded Under no circumstances shall the company be liable for any consequential damages or any indirect or incidental damages Service Agreements After the expiration of the warranty period extended service agreements are available for purchase through Michrom BioResources Inc Please contact Michrom BioResources Inc for detailed information on current service agreement options Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com SAFETY INFORMATION This manual contains warnings and precautionary statements that can prevent personal injury instrument damage and loss of data if properly followed Specific Hazards Every instrument has specific hazards associated with it Reading and complying with the following precaution instructions will help ensure the instrument s safe long term use and the operator s personal safety 1 Before supplying power to the MAGIC 2002 HPLC High Performance Liquid Chromatograph check to ensure that the voltage and fuses are set appropriately for y
54. pecial procedures are required to achieve optimum performance II Capillary Flows The Capillary Gradient Flow Range Kit includes microbore pump heads a 100 ul mixer cartridge a pre column splitter a capillary flow cell assembly and a post column splitter The capillary system is designed to run the microbore pump heads at optimal flows while delivering the desired capillary flows by utilizing a specially designed pre column splitter Figure 29 MAGIC 2002 as Capillary Unit 42 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com The MAGIC Variable Splitter The MAGIC Variable Splitter MVS is designed for use with the MAGIC 2002 Refer to Figure 30 The MAGIC Variable Splitter utilizes a unique approach for capillary HPLC The MVS consists of an off line variable splitter box a splitter tee and capillary FSLP Fused Silica Lined PEEK tubing The variable splitter box contains five different packed bed restrictors which vary in pressure drop in the same way a packed bed HPLC column does This provides a reproducible split ratio allowing a consistent flow rate across the capillary column Unlike inline splitters the MVS was designed to add no extra system volume thus allowing fast gradient separations at capillary flow rates Figure 30 MAGIC Variable Splitter Optimum Input Flow Range 50 100 ul min Effective Input Flow Range 20 200 ul min Capilla
55. r Wiring Step 3 Output 1 red black Output 2 red black ete State wal Fraction Collector Outputs Output 1 red Output 2 black Output 1 black Output 2 red This wiring configuration will show downward tick marks at the beginning of each collected peak and upward tick marks at the end of each collected peak in the EZChrom Elite data acquisition screen 50 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix I Troubleshooting Guide SYMPTOM POSSIBLE CAUSE SOLUTION No power to instrument Power cord is not properly Plug power cord into plugged into instrument instrument Power entrance fuses are blown Replace fuses with known good 5 A and 3 5 A fuses Power to instrument but no lights on pump panels Pump fuses blown at pump power entrance Replace fuses with known good 500 mA fuses Computer does not communicate with the instrument Communication cables are not connected from the computer to the instrument Plug all cables into the proper ports on the back of the computer and on the back of the instrument EZ Chrom Data System only operates in the Demo Mode The EZ Chrom license key is not plugged into the printer port on the back of the computer Plug the EZ Chrom license key into the printer port in the back of the computer The Magic Control sof
56. ressure data Fax data to Michrom BioResources Inc for diagnosis by technical support personnel 52 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com SYMPTOM POSSIBLE CAUSE SOLUTION No pressure with flow set in the Magic control software Continued Air bubble trapped in pump head Disconnect column inlet tubing Set the pumps on purge via the software and open and close both purge valves on pumps to release trapped air bubbles Wavy or choppy UV baseline trace Flow cell leak Remove column photodiode and flow cell assembly Thoroughly dry quartz flow cell and reinstall into flow cell holder and bracket Ensure proper alignment of flow cell in bracket before reinstalling assembly in column compartment Air bubble trapped in flow cell Run wash solvent at twice normal flow rate for about 30 minutes Re equilibrate at initial run conditions Lack of efficient mixing Check to ensure that a mixer cartridge of sufficient volume is installed in the mixer cartridge holder No peaks are observed when a sample is run The 10 port valve is in the inject position when the sample is loaded from the syringe Make sure that the 10 port valve is in the load position when the sample is loaded from the syringe The sample was prepared in an inappropriate solvent for the application Prior to loading the sampl
57. ressure in an HPLC system However if a leak does occur it should be corrected as soon as possible in order to maintain system integrity and operator safety It is recommended that eye and skin protection be utilized as standard laboratory attire when working on an HPLC system and that the system be shut down and returned to atmospheric pressure before attempting maintenance Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com SYSTEM REQUIREMENTS Power Gas System control Location Environmental Physical PC Requirements Controller Kit Flow Range Kit 100 240 VAC 47 63 hz 600Va Helium 20 200 PSI high pressure input Windows 95 or NT 4 0 Full Digital Control Ventilation slots air inlet and air outlet for the column forced air compartment require at least 2 inches of clearance The air inlet to the instrument is located in the front of the waste wash bottle compartment See Figure 4 page 14 The air outlet cabinet fan from the instrument is located on the top rear panel See Figure 3 page 13 Temperature 20 25 degrees Celsius Humidity 20 90 Size 19 w x 26 h x 23 d Weight 140 lbs CPU Pentium 166 MHz minimum Memory Minimum Requirements NT Win95 Clients 32MB 24MB EZServers 64MB N A Network Microsoft networking TCP IP req for client server O S Win95 service pack 1 DCOM95 1 1 req for client server WinNT 4 0 service pack
58. rough the injector and or column This obstruction may result in damage to parts of the system If possible samples should be dissolved in the starting mobile phase Solvent A or a solvent of lower chromatographic mobility than the mobile phase Solvent Requirements Many chemical manufacturers provide high purity or spectro quality reagents that are free of chemical impurities Typically HPLC grade solvents do not require filtration Choose a mobile phase that is compatible with the sample and a column that is specific for your separation requirements The structural materials that comprise the solvent fluid pathway in the MAGIC 2002 include stainless steel ceramic sapphire quartz and PEEK Caution should be taken when using strong acids strong bases non volatile buffers THF Tetrahydrofuran and Chlorinated solvents Degassing the Solvents Degas the solvents as instructed under the Basic Operation section in this manual Chapter 2 Section I Solvent Disposal All solvent waste lines on the MAGIC 2002 terminate in the solvent waste container in the waste compartment on the right side of the instrument Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Empty the waste container on a regular basis Many solvents have special disposal requirements Follow all governmental regulations when disposing of any chemical High pressure Systems and Leaks Little danger arises from the p
59. ry Column Output Flow Range 0 2 25ul min Optimum Split Flow Range 1 1 to 1 100 Effective Back pressure Range 50 4500 PSI Flow Rate Reproducibility 0 1 43 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Capillary Flow Range Kit Setup If the MAGIC 2002 was purchased as a capillary unit the system will come with the Capillary Flow Range Kit installed However if the MAGIC 2002 is set up as a Microbore unit the following procedure should be followed 1 Turn flows to the pumps off and close the solvent select valves 2 Remove both loops from the injector valve the mixer to valve tube 12 and the column inlet tube Connect the splitter tee to the injection valve port 4 using the Lite Touch fitting on the 10 cm Fused Silica Lined PEEK tubing FSLP Connect the splitter tee to the mixer output using the Lite Touch fitting at the end of the 5 cm stainless steel tubing Thread the third line from the splitter tee with micro filter in place out through the lower access port in the right side panel and connect to port R3 on the MAGIC Variable Splitter Connect the Teflon waste line to port W3 on the MAGIC Variable Splitter and place the outlet of the waste line into the MAGIC 2002 waste container Connect the sample PEEK loop or capillary trap loop of choice between ports 3 and 10 on the injection valve Connect the bypass PEEK loop betwe
60. s The MAGIC 2002 is a fully integrated HPLC system optimized for speed sensitivity recovery and capacity without compromising reproducibility or resolution Figure 2 MAGIC 2002 with doors open 2 Channel UV Vis Detector Dual Piston Pumps Mixer Body Solvent Degas System FEATURES Dual Range Piston Pumps Micro flow or analytical pump heads deliver degassed solvents at precise flows down to 1 of total flow Unique Flow Dynamics Thorough mixing rapid flush out optimized post column collection with minimum volumes Custom Columns Michrom BioResources Inc custom columns thread directly into micro or capillary flow cells eliminating extra column volume conventional columns can also be used with a standard column adaptor Microbore P N 602 25026 01 Analytical P N 602 25026 02 12 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com DESCRIPTION OF INSTRUMENT REAR PANEL Figure 3 Rear Panel Input Configuration A series of switches for On Off control of the Data System inputs External Inputs The location for the input of external signals to the data acquisition system i e auxiliary detectors The input configuration switch for the external input must be placed in the on position for the data system to accept an external input signal Data System The connection between the Data system PC board and the MAGIC 2002 25 pin A D co
61. s are stable II Operating the MAGIC 2002 The MAGIC 2002 control and data acquisition system is based on the EZChrom Elite Client Server platform The purpose of this section is to provide step by step instructions for creating or editing a MAGIC 2002 method This section also describes how to use the Instrument Status window for direct access and control of each of the MAGIC 2002 s components If the MAGIC 2002 was purchased with only the control software the information in this section will provide the necessary information for running the instrument If the system was purchased with the control and data system option detailed explanations of all the data system functions can be found in the EZChrom Elite on line manual on the CD ROM Creating or Editing a Method The method is edited in the Instrument Setup window To access the Instrument Setup window click on Method and select Instrument Setup or click on the button on the toolbar Each component of the MAGIC system has a separate tab for entering data Refer to Figures 14 19 pages 28 30 To create or edit a method click on the appropriate tab position the cursor in the appropriate field and enter the appropriate data An example of a method typically used for a 1 0mm ID column is provided on the following pages The values that can be edited are in white and items that cannot be edited are gray After creating a new method or editing an existing method save the metho
62. solution in sufficient volume to remove all of the salts and other potential interfering components without eluting the analytes of interest The trap should be back flushed with a gradient to strip the components of interest from the trap in very concentrated bands For detailed information on various traps and their uses please refer to the specific trap literature available from Michrom BioResoutrces Inc V Fraction Collector Although a wide variety of fraction collectors can be used with the MAGIC 2002 HPLC System Michrom BioResources Inc offers a Micro Fraction Collection System optimized for use with the MAGIC 2002 Please refer to the manual that comes with the fraction collector for information on operation features and options Refer to Figure 33 35 page 48 50 47 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 33 Fraction Collector Wiring Step 1 Fraction Collector DET red black MAGIC UV Outputs Channel 1 red black 48 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 34 Fraction Collector Wiring Step 2 Fraction Collector Input 1 red black MAGIC Remote I O Output C red black 49 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 35 Fraction Collecto
63. stem is completely pressurized when no more bubbling is observed in the bottles This may take as little as 10 minutes or as much as several hours depending on the type of solvents used and the solvent level in each bottle Solvent Flow to the Pump heads Prior to turning the pumps on confirm that there is solvent flow to the pump heads To check for flow at the pump heads adhere to the following procedure Turn the Solvent Select valves Refer to Figure 6 page 17 to the desired solvent open the purge valves Refer to Figure 7 page 18 and observe the Teflon waste line exiting the purge valve to the right for flow of solvent and or air bubbles Allow the Helium head pressure to force the solvents through the pumps for 3 5 minutes before closing the purge valves This procedure should be followed for the A B solvents and or the wash solvent any time the solvent inlet lines have gone dry The above process is not necessary during routine solvent changes or when refilling the solvent bottles 24 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Powering up the PC amp the MAGIC 2002 After solvent flow has been established to the pump heads power can be supplied to the computer and instrument The main power switch to the instrument can be found on the right side panel toward the rear of the instrument Refer to Figure 4 page 14 Allow 2 3 minutes for the detector to initiate diagno
64. stic routines before starting the MAGIC 2002 control software on the computer To open the control software double click on the MAGIC 2002 icon At the EZChrom Elite Client Server screen Refer to Figure 12 double click on MAGIC 2002 to access the instrument control and data acquisition windows Figure 12 EZChrom Elite Client Server Screen gt 2 EZChrom Elite Client Server Of x File Edit View Tools Help al 2 Magic 2002 Initial System Operation The initial control of the MAGIC 2002 is through the Instrument Status window To access the Instrument Status window click on Control and select Instrument Status Refer to Figure 13 page 26 The solvent select valves are normally in the Off position prior to initial system startup and need to be manually changed to select wash solvent or solvents A and B prior to enabling the pumps using the Control Instrument Status Magic Pump window Refer to Figure 24 page 34 Note It is necessary to click on status enable to verify pump head type prior to running or purging the pumps 25 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 13 Instrument Status Preview Screen ts Magic 2002 Method untitled met Data 1085pbab 010 Project Default File Edit View Method Data Sequence Analysis Control Reports Window Help a A eee am z S mle Ey Time 17 5812 Minutes Amplitude 5 223 mAU Ch
65. system pressure to stabilize at the desired flow rate and initial gradient conditions Once the system pressure is stable disconnect the flow cell outlet tube from the stainless steel bulkhead union in the ceiling of the column compartment Take the line along with the long PEEK nut and place it through the access port near the bulkhead union on the right panel of the instrument Screw the union onto the PEEK nut that is now in the access port in the right panel Remove the Manual Injection Port Refer to Figure 8 page 20 from port 1 of the injection valve and connect it to the union A syringe can now be placed in the manual injection port to take flow measurements For troubleshooting low or high flows please refer to the Troubleshooting Guide at the end of this manual Once the desired flow rate is achieved and or confirmed a standard can be analyzed to verify that the system is running properly Replacing Check Valves To replace an inlet and or outlet check valve on a pump head all pump flow and solvent flow must first be stopped Remove the old check valve from the check valve housing by loosening the associated tubing from the housing with a 4 open ended wrench and then loosen the housing itself with a 1 2 open ended wrench Refer to Figure 27 page 38 Note the orientation of the old check valve before it is removed Dry the inside of the housing and then place the new check valve inside the housing with the proper orientation
66. the MAGIC Variable Splitter to another R value setting of higher value Change the pump flow rate by factors of 2 to achieve the desired capillary flow through the column another change in restriction may also be necessary Keep in mind that the optimal pump flow conditions are 50 100 ul min Running Samples in the Split Flow Mode Once the desired flow rate has been achieved set the solvent composition to the initial conditions of the desired method Ensure the method contains the proper pump flow rate prior to running a sample Follow the operation procedures as outlined in Chapter 2 under Operating the MAGIC 2002 HI Auto sampler Although a wide variety of auto samplers can be utilized along with the MAGIC 2002 Michrom BioResources Inc offers an auto sampler optimized for use with the MAGIC 2002 Please refer to the manual that comes with each auto sampler for detailed information on operation features and options 45 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Connecting a Michrom BioResources Inc Auto sampler to the MAGIC 2002 The auto sampler will need to be wired to the MAGIC 2002 Please refer to the wiring diagram Figure 31 for connection to External Start Out and External Trigger Input on the rear panel of the instrument The auto sampler can be plumbed to the injection valve of the MAGIC 2002 several different ways Refer to the plum
67. tion first Refer to Figure 43 and Table 3 pages 58 59 and then continue on until all the modules have been properly configured as shown on the following pages Refer to Figures 44 50 pages 59 62 Figure 43 Event Configuration Screen The Event Configuration should look like this when all the events are configured Event Configuration x Configured Configured Configured Configured Configured Configured Configured Configured Colored gt 58 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com The following table lists the Events by Name the Source and the Setup for each event Table 3 Event Configuration Name Source Setup Board Input Triggered State Trigger SS420 1 In 1 Closed Ready SS420 1 Out 5 Closed MAGIC High Flow A SS420 1 Out 1 Closed MAGIC High Flow B SS420 1 Out 2 Closed Load must be typed in SS420 1 Out 3 Inject must be typed in SS420 1 Out 4 Output A must be typed in SS420 il Out 6 Output B must be typed in SS420 l Out 7 Output C must be typed in SS420 1 Out 8 Figure 44 MAGIC Pump Configuration Screen Select COM 3 for Pump A Com Port Select COM 4 for Pump B Com Port Select the Pressure Units Choices are PSI Mpa or Kg M2 Enter the maximum operating pressure The pumps will shut off if the pressure goes above this value
68. to opening the bottle To depressurize the wash bottle place the Helium valve on the Helium panel in the Off position Refer to Figure 5 turn off the Helium source to the instrument and then vent the bottle by loosening the Delrin plug on the cap insert The solvent in the wash bottle serves several functions The wash solvent washes behind the pump piston seals at a flow rate of 5 10 ul min to clear away any particulate matter or salts that may damage the seals or pistons The wash behind the pump pistons continually flows to waste and any leaks that occur because of worn primary piston seals are also vented to waste Note The piston wash continually flows at 5 10 ul min as long as the wash bottle is pressurized with a Helium source The Wash Solvent level should be checked regularly to ensure that the wash bottle solvent is never depleted as this can cause rapid loss of Helium from the source and may damage the pistons and piston seals Another function of the wash solvent is to flush the system of potential harmful mobile phases or to clean the system prior to shutdown To flush the system with wash solvent point the solvent selection valves Refer to Figure 6 page 17 inward toward the wash position 16 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com High Pressure Pumps The system contains two pumps Refer to Figure 2 page 12 an upper pump Pump A and a lower pump Pu
69. tting diode should be on whenever the power to the instrument is on The pump status flow indicator LED should be lit when the pumps are operating The Pmin pressure minimum indicator light will flash when a pump error has occurred due to low system pressure The default Pmin value is Zero therefore the pumps will continue to run even if the purge valves are open or the system has developed a leak The Pmax pressure maximum indicator light will flash when a pump error has occurred due to high pressure The default Pmax is 5000 PSI which should stop 17 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com the pumps without causing system leaks Please contact Michrom BioResources Inc before attempting to change the default Pmin and Pmax settings Outlet Check Valve Figure 7 Pump Skid Faceplate Purge Valve and Pressure Transducer Pump Head Inlet Check Valve The pump heads are located to the right of the purge valve Refer to Figure 7 The pump heads are mounted onto the pump drive by four Allen head screws The MAGIC 2002 offers either analytical heads for gradient flows between 0 1 and 5 ml min or microbore heads for gradient flows between 20 and 1000 ul min Capillary flows are also achieved using the microbore pump heads in conjunction with a pre column flow splitter for gradient flows between 0 1 and 10 ul min The two solvent lines located on the back of eac
70. tware does not communicate with the instrument The instrument needs to be started and internal diagnostics completed before communication is attempted through the control software Close the instrument control software and power off the instrument Power the instrument up and let it warm up for several minutes before attempting to communicate with it through the control software One or both solvent bottles continually sparge or bubble There is a leak in the Helium system internal to the instrument Check for Helium leaks around the bottle caps and bottle cap insert fittings Delrin plug in Wash bottle cap insert is loose or missing There is a leak in the Helium system external to the instrument Check for Helium leaks around the tank and all external fittings to the instrument Tighten fittings as necessary No solvent to pump head inlet No solvent in solvent bottles Fill solvent bottles with appropriate solvent No Helium pressure to solvent bottles Make sure Helium tank valve is open and appropriate Helium pressure is set at the tank regulator He On Off valve on inside left door of instrument is in the Off position Turn the He On Off valve to the On position 51 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com SYMPTOM POSSIBLE CAUSE SOLUTION No solvent to pump head inlet
71. xiting the column inlet line Note Pressure can be viewed from the Magic Pump Status screen or if running with the 4 channel data system option a graphical display of pressure can be obtained on Channel D by using the Preview button on the tool bar 26 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com If the flow and or pressure are inconsistent the A and B purge valves should be opened and closed until no air bubbles are observed in the purge valve exit line and the flow and pressure stabilize Change in pressure should be no greater than 50 PSI Once good flow is established for both pumps with wash solvent the purge prime procedure should be repeated for solvents A B After purging is complete close the purge valves and return the pumps to the control of the method by removing the check marks from the Purge On for both pumps setting the flow rate and B clicking on Manual Set and then clicking on Status Enabled At this time the tubing can be reconnected to the column inlet and the column allowed to equilibrate prior to running samples System Pressure Stabilization Allow the system to equilibrate at the optimum column flow rate at 50 B until pressure and baseline are stable 5 to 15 minutes Set the B to 95 for 5 to 10 minutes to flush the column then set the B to the initial conditions for the method to be executed i e 5 B and wait until the pressure and UV baseline

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