LabSpec 5 LabSpec 5 user manual Page
Contents
1. Intensity cnt 1000 Raman Shift cm When Fast Graph Settings is inactive the titles units and minimum maximum values on all visible axes are not underlined and their formatting EMTEA cannot be modified using a left click Intensity cnt Complete formatting is possible using the Format and Scale dialog window see section 8 1 page 204 for full information Note that the current status of the Fast Graph Settings i e active or inactive is also displayed in the right click menu under Fast Settings When the Fast Graph Settings is active Fast Settings will be ticked Page 197 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA Te Scientific 6 3 Selector Radio Tags for Open Data Objects When multiple data objects e g spectra images multidimensional spectral arrays of a similar type are open within a single display window the Data bar will display a selector radio tag for each object Each radio tag will be displayed in the same colour as the data object o An active object has a color filled radio w tag as shown right o An inactive object has a hollow radio tag im as shown right i Hover the mouse cursor over a radio tag to see the R data object name aN lt CTRL gt Click on a radio tag to hide show an individual object If an object is hidden the radio tag will display a red c2. 8 8 Axes The Axes dialog window allows control over what axes are displayed and how they are displayed within the data window There are two Axes dialog windows depending on the selected dimension see section 8 1 1 page 204 of the data window These two windows are discussed in turn below Available for Spectrum Video Splm Point Map Score Model 8 8 1 Axes Dialog Window for 1D and 2D Windows Options Ticks Grid Values Labels Top Left v Intensity cnt Bottom V iv M Raman Shift I em Right Intensity Style Frame Values 12 Times Main grid Labels 12 Times Sub grid Ticks Fix x gridto 100 Fix Y grid to 3DLook M Cancel 8 8 1 1 Options The five possible display axes are listed with tick boxes to select whether ticks grid lines axis values and labels are shown The fives axes are indicated on the image shown TOP right a 7mMmMr 4AmQ D lt H wzmaz BOTTOM Page 213 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA al Scientific Ticks When the Ticks box is ticked tick marks are displayed on the axis Grid When the Grid box is ticked main and sub grid lines are displayed in the spectrum window The spacing of the grid lines are automatically calculated by LabSpec 5 but can be manually assigned using the Fix X grid
3. When Photo bleaching is active i e the photo a bleaching time is gt 0s the Photo bleaching On icon is displayed For more information about Photo bleaching and how to turn it on and off please see section 3 5 4 4 page 35 Autofocus On When Autofocus is active the Autofocus On icon is AF displayed For more information about Autofocus and how to turn it on and off please see section 3 5 4 8 page 39 Intensity Correction On When Intensity Correction is active the Intensity Correction On icon is displayed For more information about Intensity Correction and how to turn it on and off please see section 3 5 4 14 page 43 Page 199 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA ee Scientific Extended Range On When an Extended Range measurement is active Aj i e the extended range function is set to either Multiwindow or Autoscanning the Extended Range On icon is displayed For more information about Extended Range acquisition and how to turn it on and off please see section 3 5 6 page 49 7 3 Detector The Detector display shows the detector temperature in degrees celsius C and on systems equipped with multiple detectors can be used to switch from one detector to another 7 3 1 Detector Temperature The detector icon is colored according to the temperature status of the active detector relative to
4. data sets e g XYZ datacubes o Trace displays a single image from a multi image single data object within the window The scroll bar on the right hand side can be used to scroll through each image corresponding to the data object the axis value for the image is displayed in the top right hand corner of the window This mode is only available for multidimensional spectral arrays and is intended for display of 3D data sets e g XYZ datacubes Dimension Select the display dimension from the Dimension drop down box The number of dimensions used for data display refers to the number of non intensity axes For example a simple spectrum is considered a 1D display because it constitutes the intensity dimension plus one other dimension the spectral axis typically Raman shift or wavelength A video image is considered a 2D display because it constitutes the intensity dimension plus two spatial axes X and Y Three display dimensions are available o 1D simple graph presentation MEZGHEZIE typically used for spectra including Spectrum Splm Point and Model windows Page 206 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA _ Scientific o 2D simple image presentation typically Score modelA used for images including video map and score windows Multiple images can be overlaid in this m
5. if a spectrum with Raman shift cm units is already open in a data window when a spectrum with wavelength nm units is opened a new data window will be created for it Page 207 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA al Scientific 8 1 2 Scale The Scale section of the Format and Scale dialog window allows control over the scaling of data within the data window Each available axis for the active data object is displayed in the Scale section with a number of options for its scaling Typical axes displayed here are o Hor the X axis typically the spectral axis for spectra or the X um axis for images o Ver the Y axis typically the intensity axis for spectra or the Y um axis for images o Intens the intensity axis for images From and To Displays the start From and stop To axis values which will be displayed These can be manually adjusted by typing in desired values and clicking OK Freeze When Freeze is ticked the axis scaling is fixed and will not be affected by scale normalization see section 4 4 1 page 91 its right click shortcut Rescale or its keyboard shortcut lt CTRL gt N The axis scaling can also be fixed using the Fast Graph Settings and selecting Fixed for the axis see section 6 2 page 197 Auto When Auto is ticked the axis will autoscale to display
6. individual data objects e g spectra images multidimensional spectral arrays to be selected from a group of open objects In addition the Data bar allows control of quick window formatting tools This is an active toolbar and the number of data object selector radio tags see section 6 3 page 198 will automatically update according to number of open objects in the currently selected window Quick Select Single or Multiple data view Quick graph settings Selector radio tags for each open data object JS VRP ARIES 6 1 Quick Select Single or Multiple Data View The Single and Multiple Data Graph icons allow fast selection of overlay mode for the active window Note that when clicked an icon will be locked t4 B down Only one icon can be active and locked xy down at a time The overlay mode can also be set using the Format and Scale dialog window see section 8 1 page 204 6 1 1 Single Data Graph ty When the Single Data Graph icon is active and locked only the active data object will be displayed in the window For example if there are three spectra open only the active spectrum will be displayed Selecting a different spectrum using the Data bar see section 6 3 page 198 will cause the selected spectrum to be displayed Page 195 Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA Explore the future HORIBA os Scientific In the example shown
7. line longer or shorter To clear the existing line and create a new default line at the center of the video image do one of the following o Click on the Center Cursors icon in the ae ae Icon bar see 4 4 3 page 92 o Right click and select Center cursor The Shift Hor Line and Adjust Hor Line cursors should be used in conjunction with the Mapping Properties dialog window see section 4 5 5 page 97 Available for Video 5 19 Rectangular Mapping o Activates the Rectangular Mapping cursor allowing a rectangular area for mapping to be defined on the active video image When this icon is active and the mouse is hovered N gt over the existing rectangle the cursor changes to the Shift Rectangle cursor Page 187 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA os Scientific Left click and drag with the Shift Rectangle cursor to move the line to the desired position on the video image When this icon is active and the mouse is hovered N gt PE Xa ii Pal over the rectangle drag points the cursor changes to the Adjust Rectangle cursor Left click and drag with the Adjust Rectangle cursor to adjust the rectangle size To clear the existing rectangle and create a new default rectangle at the center of the video image do one of the following o Click on the Center Cursors icon in the RE we Icon bar see 4 4
8. the temperature set in the Detector dialog window see section 3 5 7 page 57 o Actual temperature is between set E temperature and 5 C above the set temperature detector can be used o Actual temperature is between 5 C and E 10 C above the set temperature Detector can be used but data acquired in this state may exhibit slightly reduced quality signal to noise o Actual temperature is greater than 10 C E above the set temperature it is recommended that the detector is not used Note that use of a detector at high temperature will not damage it but data acquired in this state will exhibit significantly reduced quality signal to noise Page 200 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA a Scientific 7 3 2 Switching Detector On systems equipped with multiple detectors click v JY_32 on the detector icon to view a list of available Symphony InGads detectors The currently active detector is 7u indicated with a tick To switch detector select the desired detector from v JY 32 the list of available detectors Note that a detector initialization message may be displayed when the Ea u detector is switched 7 4 Progress Bar The Progress Bar indicates whether a hardware software process is active and displays the approximate progress of an active process It is also possible to temporarily pause a process usi
9. to and Fix Y grid to options in the Style section of the Axes dialog window see section 8 8 1 2 page 214 Values When the Values box is ticked axis values are displayed along the axis Labels When the Labels box is ticked the axis title and units are displayed adjacent to the axis Custom labels can be used by typing in the desired axis label in the Labels text box To restore the default labels for an axis delete the text in the box 8 8 1 2 Style The Style section allows the formatting of the axis components frame ticks grid lines values and labels to be set Frame Select the line style for the axes frame using the Frame drop down box Main grid Select the line style for the main grid lines using the Main grid drop down box Sub grid Select the line style for the sub grid lines using the Sub grid drop down box Fix X grid to When the Fix X grid to box is ticked the main grid lines for the X axis will be spaced at the interval displayed in the box To adjust the spacing type the desired grid line spacing into the text box 3D look When the 3D look box is ticked the axes frame is displayed in a 3D style Values Select the font style for the axis values from the Values drop down box Labels Select the font style for the axis values from the Labels drop down box Ticks Select the line style for the ticks using the Ticks drop
10. 3 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA a Scientific 8 1 Format and Scale The Format and Scale dialog window allows control over the window display format and the data scaling within the window Note that depending on the data window selected the Format and Scale dialog window may slightly differ from that shown below Format and Scale Style Internal Help Extemal C Cancel Mode Overlay Dimension 1D M Use scroll bars V Always show scroll bars I One data per view Preserve scale ratio IF Use data only with the same X axis Scale From To Freeze Auto Rel Log 99 588 1500 46 EH 2 HS foo COCO Available for Spectrum Video Splm Point Map Score Model 8 1 1 Style The Style section of the Format and Scale dialog window allows control over the appearance of the data window and how data is displayed within it Internal Select the internal area color and fill option from i zeiee ees the Internal color drop down box Note that when a data window is copied as an image it will be copied with the default internal white background INTERNAL AREA 2 E a The internal area is shaded in the image shown right External Select the external area color and fill option from Spim the External color drop down box Note that when a data window is copied as an image it will be copied
11. 3 page 92 o Right click and select Center cursor The Shift Rectangle and Adjust Rectangle cursors should be used in conjunction with the Mapping Properties dialog window see section 4 5 5 page 97 Available for Video 5 20 Line Mapping A Activates the Line Mapping cursor allowing a line for line mapping to be defined on the active video image When this icon is active and the mouse is hovered i gt over the line the cursor changes to the Shift Line cursor Left click and drag with the Shift Line cursor to move the line to the desired position on the video image When this icon is active and the mouse is hovered N gt over the line drag points at each end of the line the cursor changes to the Adjust Line cursor Left click and drag with the Adjust Line cursor to make the line longer or shorter or to rotate the line about the opposite drag point To clear the existing line and create a new default line at the center of the video image do one of the following Page 188 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA Scientific o Click on the Center Cursors icon in the ae ae Icon bar see 4 4 3 page 92 o Right click and select Center cursor The Shift Line and Adjust Line cursors should be used in conjunction with the Mapping Properties dialog window see section 4 5 5 page 97 Avail
12. AXIS SHOULD HAVE UNITS RUNNING FROM NEGATIVE AT THE TOP THROUGH TO POSITIVE AT THE BOTTOM AS DISPLAYED RIGHT Available for Spectrum Video Splm Point Map Score Model 8 6 Image Colors The Colors dialog window allows control of the color palettes used for image rendition and adjustment of image brightness and contrast Colors Colors Palette Red Oi Kel Zel is CE CE ma Help IV Green CHE EC we CEH CECEN 7 Ble Cancel Contrast Brightness M Neg Q9 Calc RCGCB B Available for Spectrum Video Splm Point Map Score Model Page 210 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA a Scientific 8 6 1 Colors LabSpec 5 uses an RGB palette for its image rendition by default red R green G and blue B components are used to create the colored images displayed When a color Red Green or Blue is ticked it will be used to create the colored images For correct color rendering of images based on the selected color palette see section 8 6 2 page 211 ensure that all three colors Red Green and Blue are ticked When Neg is ticked the negative image will be displayed for the selected color palette 8 6 2 Palette Available color palettes are listed in this section Left click on one of the palette radio buttons to select the palette There
13. HORIBA ae Scientific Left click and drag with the Move Delete Points Video video cursor to move the existing multipoint position to a new position To clear all existing points and set point 1 at the center of the video image do one of the following o Click on the Center Cursors icon in the re eit Icon bar see 4 4 3 page 92 o Right click and select Center cursor Note that the automated multipoint acquisition is RR a started by using the Mapping Acquisition icon see section 4 5 4 page 96 The data is acquired as a multidimensional spectral array with the standard Splm Point and Map windows Available for Video Page 186 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA Scientific 5 18 Hor Line Mapping Activates the Horizontal Line Mapping cursor allowing a horizontal line for line mapping to be defined on the active video image When this icon is active and the mouse is hovered R gt pe over the line the cursor changes to the Shift Hor Line cursor Left click and drag with the Shift Hor Line cursor to move the line to the desired position on the video image When this icon is active and the mouse is hovered N gt lt gt over the line drag points at each end of the line the cursor changes to the Adjust Hor Line cursor Left click and drag with the Adjust Hor Line cursor to make the
14. able for Video 5 21 Circle Mapping o Activates the Circle Mapping cursor allowing a circle for mapping to be defined on the active video image When this icon is active and the mouse is hovered N gt over the line the cursor changes to the Shift Circle cursor Left click and drag with the Shift Circle cursor to move the line to the desired position on the video image When this icon is active and the mouse is hovered ls gt gt Xa i Faj over the circle area drag points at each edge of the circle area the cursor changes to the Adjust Circle cursor Left click and drag with the Adjust Circle cursor to adjust the circle size To clear the existing circle and create a new default circle at the center of the video image do one of the following o Click on the Center Cursors icon in the RE we Icon bar see 4 4 3 page 92 o Right click and select Center cursor The Shift Circle and Adjust Circle cursors should be used in conjunction with the Mapping Properties dialog window see section 4 5 5 page 97 Available for Video Page 189 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA os Scientific 5 22 Ver Line Mapping Activates the Vertical Line Mapping cursor allowing a vertical line for line mapping to be defined on the active video image When this icon is active and the mouse is hovered Is gt pe o
15. all data open in the data window The axis scaling can also be set to autoscaling by using the Fast Graph Settings and selecting Auto for the axis see section 6 2 page 197 Rel When Rel is ticked all data open in the data window will be displayed with identical minimum and maximum intensities The scale values displayed for the axis will correspond to the active data object The data shown right are displayed with normal 1007 scaling 30 g 60 5 404 4 4 20 4 o AA A 500 i 1 000 1500 Raman Shift cm The data shown right are displayed with relative 25 scaling The active spectrum is 24 g 154 E 104 s 0 500 1 000 1500 Raman Shift cm The axis scaling can also be set to relative scaling by using the Fast Graph Settings and selecting Relative for the axis see section 6 2 page 197 Page 208 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA a Scientific Log When Log is ticked the axis will be displayed with logarithmic scaling rather than linear scaling 8 2 Fast Settings When Fast settings is ticked the Fast Graph Settings are active see section 6 2 page 197 Left click on Fast settings to tick activate or untick deactivate this function Available for Spectrum Video Splm Point Map Score Model 8 3 Rescale Left click on Rescal
16. are two types of palette o True color with this palette each color gag component is displayed in its true color In the case of a video image the three components red green and blue are displayed in their individual colors to yield a composite colored image In the case of a map image created from a multidimensional spectral array the cursor intensity image corresponds to the cursor color For example the image generated using the red map analysis cursor will be displayed in a one color red scale In the case of a score image created from a multidimensional spectral array the score image corresponds to the reference component spectrum displayed in the Model window For example the score image for a red spectrum in the Model window will be displayed in a one color red scale This palette should be used for video images and map score images where multiple components are overlaid Page 211 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA E Scientific o False color with one of these palettes image pixels are displayed in different colors depending on their intensity These palettes are useful when an image has regions of both high and low intensity which can be hard to visualize with a traditional true color palette These palettes should only be used for representation of a single component e g a single cursor int
17. d gt fm the mouse is hovered over an existing polygon definition point the cursor changes to the Move Delete Polygon Points cursor Page 191 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA al Scientific Left click with the Move Delete Polgyon Points Video cursor to delete the existing polygon definition point Left click and drag with the Move Delete Polygon Points cursor to move the existing polygon definition point to a new position Page 192 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA ae Scientific To clear all existing polygon definition points and set a single point at the center of the video image do one of the following o Click on the Center Cursors icon in the Re ae Icon bar see 4 4 3 page 92 o Right click and select Center cursor Note that the polygon shape is created by locating nearest neighbours to each polygon definition point Slight adjustment of polygon definition points may be required to obtain the desired polygon mapping shape The Add Polygon Points and Move Delete Polygon Points cursors should be used in conjunction with the Mapping Properties dialog window see section 4 5 5 page 97 Available for Video 5 24 Axes3D K Activates axis drag points on a 3D image allowing its position
18. d profiles maps will be those selected in Options gt Unit see section 3 4 1 page 22 typically counts cnt or counts per second cnt s The units for video images will be arbitrary units a u X Y Z spatial position in a profile or image The units will be micrometers um o P spectral X axis width between a paired set of map analysis cursors The units will be those selected in Options gt Unit see section 3 4 1 page 22 typically Raman shift cm or wavelength nm o W approximate peak width as indicated by the Peak cursor The units will be those selected in Options gt Unit see section 3 4 1 page 22 typically Raman shift cm or wavelength nm Page 202 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA a Scientific 8 Right Click Menus Right mouse clicking on the data windows will display a menu which typically will allow access to additional dialog windows provides shortcuts to other functions within LabSpec 5 or provides information about the current configuration The right click menu is active and its appearance and content will update according to the currently selected window For example the options appearing for a Spectrum window will differ from those appearing for a video image At the end of each function s description a list of windows where the function is available in the right click menu is give
19. down box Fix Y grid to When the Fix Y grid to box is ticked the main grid lines for the Y axis will be spaced at the interval displayed in the box To adjust the spacing type the desired grid line spacing into the text box Page 214 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA Scientific 8 8 2 Axes dialog Window for 3D Windows Options e C I Ticks c c J Values vie c I Label 2 g SwapX and Re center image Style Frame v Values 12 Times B Ticks M Labels 12 Times Help Cancel 8 8 2 1 Options The schematic in the Options section illustrates the seven available axes six surrounding the 3D image and one for intensity Each axis is configured individually Select an axis by clicking the appropriate radio tag amp When the tick box is ticked the three forward axes are displayed in front of the 3D image Ticks When the Ticks box is ticked tick marks are displayed on the axis Values When the Values box is ticked axis values are displayed along the axis Labels When the Labels box is ticked the axis title and units are displayed adjacent to the axis Custom labels can be used by typing in the desired axis label in the Labels text box To restore the default labels for an axis delete the text in the box Swap X and Y Click on Swap X and Y to reverse the position
20. e to rescale the active window so that all data is visible In normal operation all axes are affected but note that an axis with scaling set to Fixed will not be rescaled To rescale an axis ensure that Freeze or Fixed scaling is turned off see section 8 1 page 204 This function can also be activated with the Scale Normalization icon see section 4 4 1 page 91 or with the lt CTRL gt N keyboard shortcut Available for Spectrum Video Splm Point Map Score Model 8 4 Swap X axis Click on Swap X axis to reverse the X axis display CAUTION WHEN AN XY MOTORIZED SAMPLE STAGE IS PRESENT THE AXIS DISPLAY FOR VIDEO IMAGES SHOULD ALWAYS BE LEFT IN THE DEFAULT CONFIGURATION TO ENSURE CORRECT MAPPING AND EXTENDED VIDEO IMAGING ACQUISITION THE X AXIS SHOULD HAVE UNITS RUNNING FROM NEGATIVE ON THE LEFT HAND SIDE THROUGH TO POSITIVE ON THE RIGHT HAND SIDE AS DISPLAYED RIGHT Y um Available for Spectrum Video Splm Point Map Score Model Page 209 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA ves Scientific 8 5 Swap Y axis Click on Swap Y axis to reverse the Y axis display CAUTION WHEN AN XY MOTORIZED SAMPLE STAGE IS PRESENT THE AXIS DISPLAY FOR VIDEO IMAGES SHOULD ALWAYS BE LEFT IN THE DEFAULT CONFIGURATION TO ENSURE CORRECT MAPPING AND EXTENDED VIDEO IMAGING ACQUISITION THE Y
21. ensity map image or a single score image 8 6 3 Contrast and Brightness Left click and drag the Brightness slider to adjust 9g the brightness for the image display Left click and drag the Contrast slider to adjust the contrast for the image display If All is selected the brightness and contrast adjustments will affect the complete RGB palette If one of R G or B is selected the brightness and contrast adjustments will only affect the selected component R red G green B blue of the RGB palette Click on the Contrast and Brightness Initialization icon to reset the brightness and contrast settings to their default values Note that this operation needs to be done individually for All R G and B E 8 7 Center Cursor Left click on Center cursor to center the active cursor s in the active window This function is useful when cursors are not visible in a window because their position lies outside the range of the window Right click and select Center cursor and the cursor s will be immediately visible in the center of the window This function can also be activated with the Center Cursors icon see section 4 4 3 page 92 Available for Spectrum Video Splm Point Map Score Model Page 212 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA Scientific
22. iconductor Scientific HORIBA HORIBA _ Scientific height FWHM of the peak The Peak cursor displays the X axis position S intensity I and approximate full width at half maximum height W of the peak at the cursor position Video Map Score windows o Cross cross hair cursor displaying the X axis position X and Y axis position Y of the cursor and pixel intensity I at the cursor position For the Map and Score windows the spectrum associated with the cursor position will be displayed in the Point window o Rect rectangular cursor resizeable by left clicking and dragging the drag points displaying the X axis position X and Y axis position Y of the bottom right hand corner of the rectangular cursor For the Map and Score windows the average spectrum from within the rectangle is displayed in the Point window Selected Select the display formatting for the cursor when it is selected i e active using the Selected drop down box Deselected Select the display formatting for the cursor when it is not selected i e inactive using the Deselected drop down box Transparent When Transparent is ticked the cursor is displayed in a transparent mode allowing features behind the cursor to be visualized Fixed width When Fixed width is ticked the width between Double cursors is fixed to its current value In this mode both cursors will move together when dragged by the mouse To adjus
23. ine Select the line style from the Line drop down box for the line surrounding the scale bar Fill Select the fill colour from the Fill drop down box for the scale bar Font Select the font style from the Font drop down box for the scale display text Note that the color of the text is set through the Color drop down box Color Select the color from the Color drop down box for the scale display text Available for Video Map Score 8 17 Imposition The Imposition dialog window allows information from the multidimensional spectral array Map and Score windows to be superimposed on the active video camera image The display color is automatically assigned by LabSpec 5 to match the color of the object being superimposed To start the selected superimposition click OK and then activate a Map or Score window Imposition V Show data limits F Show data points Help F Show data name Cancel Show index Overlay images Font fia Times v Page 222 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA
24. n The possible windows are as follows Spectrum The spectrum display window for individual spectra acquired using the real time display RTD acquisition ie ie and spectrum acquisition i it modes Video The video display window for optical images acquired with the integrated microscope camera s Spim The overlay of all spectra within a multidimensional spectral array Point The spectrum at the current cursor position within a multidimensional spectral array Map The cursor intensity profile image display created from a multidimensional spectral array Score The score profile image created by DCLS modelling of a multidimensional spectral array Model The reference component spectra used for DCLS modelling of a multidimensional spectral array To display the right click menu right click MEZZEST anywhere within the active window Left click ona EAER menu item to open a new dialog window or run the Lattasllinge function i 1 Swap X ats Swap Y axis Image colors 3 Centre cursor J Axes Legend 7 2 Spectrum d Ra Cursor i Rescale Note that setting a function in a right click menu dialog window will only set that function for the active window It is possible to have different settings for different windows For example a Spectrum and Point window can have different format and scale settings by applying different settings through the Format and Scale dialog window for each window Page 20
25. ng the Progress Bar Pause icon When a process is active the Progress Bar lt gt u SG displays a moving circle icon and blue bars which indicate the current progress j j 0 100 complete complete Current completion state Left click on the Pause icon to pause the current BEE process The process will continue until the current section of the process is completed and it will then pause The Pause icon will change into the Start icon Left click on the Start icon to re start a paused SCT process The Start icon will change into the Pause icon When no process is active the Progress Bar is empty 7 5 Cursor Values The Cursor Value display shows a range of information depending on the type of cursor which is active e g the X and Y axis positions of the cursors the spectrum profile image intensity at the cursor position width between two cursors and approximate peak width The following symbols are used to identify information displayed in the Cursor Value display Page 201 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA ee Scientific o S spectral X axis position in a spectrum The units will be those selected in Options gt Unit see section 3 4 1 page 22 typically Raman shift cm or wavelength nm o l intensity The units for spectra and cursor generate
26. ode o 3D for pseudo 3D representation of 2D data where all three axes intensity plus two spatial are visible Only a single image component can be displayed in this mode eS g i D F 5 Note that 3D images are configured using the right click menus Axes see section 8 8 2 page 215 and 3Dimage see section 8 18 page 224 Use Scroll Bars When Use scroll bars is ticked scroll bars will be displayed if part of the data is off scale e g not actually displayed in the data window Always Show Scroll Bars When Always show scroll bars is ticked the scroll bar display section of the data window will always be present even if all data is displayed within the window The scroll bar s will only become active if part of the data is off scale e g not actually displayed in the data window One Data per View When One data per view is ticked each data object associated with the data window will always open into a new data window In this case it is not possible to overlay objects in a single window Preserve Scale Ratio When Preserve scale ratio is ticked the aspect ratio will be retained Typically this function is used for image display only Use Data Only with Same X Axis When Use data only with same X axis is ticked only data objects which share the same X axis units will be opened within the window Objects with other X axis units will be opened into a new data window For example
27. or the legend frame using the Frame drop down box Text Select the font style for the legend text from the Text drop down box Available for Spectrum Spim Point Model Page 216 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA _ Scientific 8 10 Spectrum The Spectrum dialog window allows control of the spectrum display format Spectrum Options Sule Ciy CLA CO fib I Use same style for all spectra Line v Point size f1 Style Select the display style from the following options o Line An o Dot o Bar tn Page 217 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA a Scientific Use same style for all spectra When Use same style for all spectra is ticked all spectra in the current window will share a common line style based upon the current setting in the Spectrum dialog window If Use same style for all spectra is not ticked then the line style setting in the Spectrum dialog window is applied to the active spectrum only In this mode it is possible to have multiple spectra displayed with different line styles Line Select the spectrum display color and where appropriate line style for the spectrum using the Line drop down box Point size Select the
28. point size for the Dot spectrum display Note that the point color is set in the Line drop down box Available for Spectrum Spim Point Model 8 11 Cursor The Cursor dialog window allows control of the cursor behaviour and display format Pointer Options Style Line v Help Selected oneal Deselected I Transparent J Fixed width F Add position to custom info Style Select the cursor style from the Style drop down box The following cursor styles are available Spectrum Model windows o Line single vertical line cursor displaying the X axis position S of the cursor and the intensity I of the spectrum at the cursor position o Cross cross hair cursor displaying the X axis position S and Y axis position I of the cursor o Level cross hair cursor which tracks the intensity of the spectrum displaying the X axis position S and Y axis position l of the cursor In this case the Y axis position is equivalent to the spectrum intensity at the cursor position o Double two vertical cursors displaying the X axis position S of each cursor and the width between the two cursors W o Peak three linked vertical cursors the central one locking to the maximum intensity pixel in a peak and the outer two locking to the pixels closest to the full width at half maximum Page 218 Explore the future Automotive Test Systems Process amp Environmental Medical Sem
29. right there are three open e spectra but only the active spectrum is n displayed Cc 500 1 doo 1 500 Raman Shift cem 6 1 2 Multiple Data Graph t When the Multiple Data Graph icon is active and locked all open data objects will be displayed in the window For example if there are three spectra open all three will be displayed in an overlay mode in the window In the example shown right there are three open spectra all of which are displayed F T T T T 500 1 000 Raman Shift em T 1500 Note that the overlay mode Overlay Tile Stack Projection or Trace used with the Multiple Data Graph icon will be the mode set in the Format and Scale dialog window see section 8 1 page 204 To adjust the overlay mode right click and select Format and Scale and select the desired mode from the Mode drop down box Page 196 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA al Scientific 6 2 Fast Graph Settings When the Fast Graph Settings icon is active and locked the formatting of axis units and scaling can be quickly modified When Fast Graph Settings is active the titles units and maximum minimum values on all visible axes are underlined Left click on an underlined item to modify its Spectrum spectrum formatting by selecting a format from the drop down list
30. ross o In the example shown right there are three open spectra all of which are displayed TT a T T T T T 500 1 000 1 500 Raman Shift em o In the example shown right there are w three open spectra but one has R been hidden using lt CTRL gt Click and is not displayed The spectrum can be shown again by lt CTRL gt Clicking on its radio tag T T T T 500 1 000 Raman Shift cm T 1 500 Page 198 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA a Scientific 7 Status Bar The Status bar displays information about system status e g progress of an acquisition active options e g intensity correction or extended range settings and cursor values Estimated left time 0 00 05 sec fees AE BES GA 273 BENNE S 1131 67 1 12847 system active detector progress bar cursor values messages options 7 1 System Messages System messages show information relevant to the current operation Typical examples include the estimated time remaining until completion of a measurement current status operation of the autocalibration routine or current status operation of the spectrometer 7 2 Active Options The Active Options icons indicate whether certain data acquisition options are active typically these are options which have a significant effect on the measurement Photo bleaching On
31. s of the X and Y axes in the 3D image display Re center image Click on Re center image to return the image to its default position in the display window This function is useful if the image position has been modified using the Axes3D icon see section 5 24 page 193 8 8 2 2 Style The Style section allows the formatting of the axis components frame ticks values and labels to be set Frame Select the line style for the axes frame using the Frame drop down box Page 215 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA an Scientific Ticks Select the line style for the ticks using the Ticks drop down box Values Select the font style for the axis values from the Values drop down box Labels Select the font style for the axis values from the Labels drop down box 8 9 Legend The Legend dialog window allows control of the legend display and formatting Legend i Options Show Single f Multi oa Text 12 Times Help Cancel The legend is displayed for spectral windows and 100 cae m spectrum2 shows the data object name and its display color 30 Intensity cnt 500 l 1 000 1500 Raman Shift cm Show Tick the boxes for Single and Multi to activate the legend for single and overlay display modes respectively Frame Select the line style f
32. shape and perspective to be modified When this icon is active four drag points are M activated on the 3D image gt bad amp F G 5 Left click and drag the drag points to adjust the position shape and perspective of the image Drag Point 1 Dragging up down adjusts the size of the vertical intensity axis Dragging left right skews the image about its center point keeping the left hand side and right hand side vertical axes static Page 193 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA ee Scientific Drag Point 2 Dragging up down left right adjusts the image position within the display window Drag Point 3 Dragging up down left right skews the image keeping the back and right hand side vertical axes static Drag Point 4 Dragging up down left right skews the image keeping the back and left hand side vertical axes static To restore the 3D image to its default display right click and select Axes and then click on Re center image see section 8 8 2 page 215 Available for Video Solm Map Score when displayed in 3D mode Page 194 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA Scientific 6 Data Bar The Data bar located on the right hand side of the LabSpec 5 graphical user interface GUI allows
33. t the width of the Double cursors ensure that Fixed width is unticked Allow selected points When Allow selected points is ticked multiple discrete points within a map or score profile image can be selected When lt CTRL gt is held down the mouse cursor N op changes to the Select Point cursor lt CTRL gt click to select a position in the map or score profile image Multiple positions can be simultaneously selected by clicking additional positions When lt CTRL gt is held down and the mouse cursor p m is hovered over an existing selected point the mouse cursor changes to the Remove point cursor lt CTRL gt click to delete an existing selected point The average spectrum from the selected points will be displayed in the Point window Page 219 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA ows Scientific Add position to custom info When Add position to custom info is ticked the cursor values are included within the Custom tab of the parameters information dialog window see section 4 4 5 page 92 Available for Spectrum Video Map Score Model 8 12 Red Cursor The Red Cursor dialog window allows control of the cursor behaviour and display format for the red SplmRed map analysis cursor See section 5 2 page 165 The Red Cursor dialog window is identical to the standard Cursor dialog windo
34. ver the line the cursor changes to the Shift Ver Line cursor Left click and drag with the Shift Ver Line cursor to move the line to the desired position on the video image When this icon is active and the mouse is hovered ls gt t over the line drag points at each end of the line the cursor changes to the Adjust Ver Line cursor Left click and drag with the Adjust Ver Line cursor to make the line longer or shorter To clear the existing line and create a new default line at the center of the video image do one of the following o Click on the Center Cursors icon in the RE zE Icon bar see 4 4 3 page 92 o Right click and select Center cursor The Shift Ver Line and Adjust Ver Line cursors should be used in conjunction with the Mapping Properties dialog window see section 4 5 5 page 97 Available for Video 5 23 Polygon Mapping o Activates the Polygon Mapping cursor allowing a polygon for mapping to be defined on the active video image When this icon is active and the mouse is hovered N gt over the video image the cursor will change to the Add Polygon Points cursor Page 190 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA ails Scientific Left click with the Add Polygon Points cursor to video add a polygon definition point to the video image When the Add Polygon Points cursor is active an
35. w described above see section 8 11 page 218 The map analysis cursor should be operated in Double mode It is advisable that the color is retained as red to avoid confusion Available for Splm Point 8 13 Green Cursor The Green Cursor dialog window allows control of the cursor behaviour and display format for the green SplmGreen map analysis cursor See section 5 2 page 165 The Green Cursor dialog window is identical to the standard Cursor dialog window described above see section 8 11 page 218 The map analysis cursor should be operated in Double mode It is advisable that the color is retained as green to avoid confusion Available for Splm Point 8 14 Blue Cursor The Blue Cursor dialog window allows control of the cursor behaviour and display format for the blue SplmBlue map analysis cursor see section 5 2 page 165 The Blue Cursor dialog window is identical to the standard Cursor dialog window described above see section 8 11 page 218 The map analysis cursor should be operated in Double mode It is advisable that the color is retained as blue to avoid confusion Available for Splm Point Page 220 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA Scientific 8 15 Image The Image dialog window allows the image color and smoothing options to be set Options f i G C Sye G
36. with the default external white background Intensity ent The external area is shaded in the image shown right Page 204 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA _ Scientific Mode Select the overlay mode for data objects e g spectra images multidimensional spectral arrays from the Mode drop down box A number of overlay modes are available o Single display only the currently active 2 _ B data object in the window Intensity cnt o Overlay display all open data objects in the window g 2 a o Tile display all open data objects in Spectrum spectrum separate tiled sections within the window Intensity ent Intensity cnt ooo Raman Shift cra ms t 1000 1000 Raman Shift cm Raman Shift cra Intensity ent Intensity ent o Stack display all open data objects in Spectrum spectrum the window offsetting each by a certain amount Intensity cnt Page 205 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA oes Scientific o Projection displays a series of images corresponding to the open data object in separate tiled sections within the window This mode is only available for multidimensional spectral arrays and is intended for display of 3D
37. yE W Help cee ye Cancel Color gt Style Select the smoothing interpolation style from the available options o No smoothing image is displayed in raw pixelated form o X smoothing image is displayed with smoothing interpolation in the X dimension o Y smoothing image is displayed with smoothing interpolation in the Y dimension o XY smoothing image is displayed with smoothing interpolation in the X and Y dimensions KS T S Color Select the image display color for the active image component from the Color drop down box Note that the color selected is only displayed when the image is displayed in True Color mode see section 8 6 page 210 for full information about image colors When the color is changed for a DCLS modelling score image the reference component spectrum display in the Model and Point windows will be automatically updated Similarly the score image color will be automatically updated if the reference component spectrum color is modified in the Model window Available for Video Map Score Page 221 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA es Scientific 8 16 Scale Bar The Scale Bar dialog window allows control of display formatting for the image scale bar Scale Bar r Options Line y Help Fi L iz Cancel Font 14 Times N z Color L
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