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Model 370 Series Steri-Cycle CO2 Incubator Direct Heat with

1. a 45 1 4 2 a3 RH 3g 42 6 a5 REI 44 D 40 43 4RS 485 RS 485 U JI 1 42 a JI 2 FZ J1 3 43 Ji 4 45 gt 1 5 5 55 21 6 7 4 L TEMP 4 ANALOG CO2 n DUTPUT 5 BOARD 7 02 5 J13 1 5 JI3 2 47 5 J13 3 4 e old 213 4 4 J13 5 450 J1 341 sl J13 7 e J13 8 27 1 J7 2 455 7 3 J7 4 2 7 5 58 J7 6 e 0 t 8 REMOTE ALARM CONNECTIONS shown in alarn state OPTIONAL RS 485 OUTPUT KIT 1800085 OPTIONAL RH SENSOR KIT 1900081 OPTIONAL ANALOG OUTPUT KITS 190512 4 to 20 190543 0 to SVDC 190544 to IVDC OPTIONAL GAS GUARD KIT 1900086 2 PRESSURE SWITCH 1 TANK EMPTY 360213 PRESSURE SWITCH 2 TANK EMPTY 360213 SOLENOID 3 WAY 12VDC 250121 MODEL 381 IR SYSTEM 231204 Electrical Schenotic Model 371 and 381 Heat Sterilization Incubator 371 70 0 D REV 5 Page 2 of 3 Thermo Scientific Steri Cycle CO Incubator 9 5 77
2. 40 52 5 e 44 REMOTE ALARM CONNECTIONS 41 1 ot 43 RJ 11 shown in olorn state II 39 2 1 e 42 x 6 43 as REI AL 24 OPTIONAL RS 485 OUTPUT KIT 242 43 185 485 E 45 0 lt 2 RS 485 6 f 4 47 J1 2 MM r h JI 3 4 OPTIONAL RH SENSOR KIT 48 5 1900091 DASS RUP 3598 49 J1 6 m E 50 2 51 MP TEMP _ 2 52 9 7 ANALOG C02 OPTIONAL ANALOG OUTPUT KITS 10 OUTPUT 190512 4 to 20nA 23 J BOARD 190543 0 to SVDC at 190544 0 to IVDC S Q 02 55 56 46 OPTIONAL GAS GUARD KIT 13 1 1900086 N C 97 213 2 7 NLoNer PRESSURE SWITCH 1 TANK EMPTY 360213 58 48 H 13 3 e a old PRESSURE SWITCH 2 TANK EMPTY 360213 8 J13 4 43 60 213 5 EU 51 13 6 4 SOLENOID 3 WAY 12VOC 61 33 52 250121 52 eae MODEL 380 63 IR CO2 SYSTEM Senis L 231204 RA dua e i JI 3 i S sb 65 J7 4 JE P 5 7 6 67 68 o 26 a 69 70 8 71 N 72 73 74 Electrical Schenatic Model 370 and 380 Heat Sterilization Incubator 370 70 0 D REV Poge 2 of 3 9 2 Steri Cycle CO Incubator Thermo Scientific
3. WIRE REFERENCE CHART 78 ca coor COLOR 79 1 16 BROW 21 18 RED 42 2273 BLACK 80 2 16 BLUE 22 18 GREE 43 2273 RED 3 18 GRN YEL 23 18 GRANGE 44 2273 GREEN 81 4 16 BLAC 24 18 CREE 45 22 BLACK 82 5 18 WHITE 25 22 BLUE 46 20 RED 93 6 18 BLAC 26 22 BLUE 47 20 RED j 16 YELLOW 27 22 BROW 48 20 BLLE 84 8 18 ORANGE 28 22 BROW 49 20 BLLE 85 9 18 RED 29 22 YELLOW 50 20 ORANGE 0 18 BLUE 30 22 YELLOW 51 20 ORANGE 86 l 18 RED 31 22 RED 52 20 BLACK 87 2 32 22 RED 53 18 BROWN 39 sss s eS 33 22 GREE 54 24 4 BLACK 4r les sss 34 22 GRAY 55 24 4 WHITE 88 24 35 22 PURPLE 56 24 BLACK 90 6 36 22 GRANGE 57 24 4 RED 7 18 ORANGE 37 2273 BLAC 58 24 4 GREEN x 8 18 BLACK 3 22 3 RED 59 92 9 18 YELLOW 38 22 3 WHITE 60 93 20 40 22 2 RED 41 22 2 BLACK 94 95 96 97 98 99 00 02 MODEL DESCRIPTION 370 STERI CYCLE CO2 INCUBATOR 03 380 STERI CYCLE I R CO2 INCUBATOR 3598 STERI CYCLE T C CD2 INCUBATOR 04 3589 STERI CYCLE I R 02 INCUBATOR 05 35B4 STERI CYCLE CO2 INCUBATOR RH DISPLAY 3585 STERI CYCLE I R CO2 INCUBATOR RH DISPLAY UO 3540 STERI CYCLE T C CO2 INCUBATOR 115 VOLT 07 3542 STERI CYCLE I R CO2 INCUBATOR 115 VOLT
4. e 000000000000000000000000 e Stacking Stacking bracket bracket Bottom Incubator back Figure 1 8 Installed Brackets on Back of Unit Z 10 11 12 15 14 Thread one 1 4 x 20 bolt and washer included with the stacking brackets into the hole behind the cover plate Do not tighten Refer to Figure 1 9 Remove the cover plate from the same area on the other side of the top unit Thread the other 1 4 x 20 bolt and washer into this hole Tighten the bolts on both sides Assemble the door hinges to the unit Secure with the screws Plug the door cord into the connector as previously Secure the cover plate Cover plate and bolt hole to secure units together Figure 1 9 Bolt Hole Install the cover plate on the other side of the unit The stacked incubators are ready for service Thermo Scientific Preliminary Cleaning Installing the Shelves Thermo Scientific Section 1 Installation and Start Up Remove vinyl from shelf channels duct sheets and air duct if present 2 Using a suitable laboratory disinfectant clean all interior surfaces Caution Before using any cleaning or decontamination method except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment Accidental spills of hazardous materials on or inside this unit ar
5. right door swing Figure 5 2 Door Swing Thermo Scientific Steri Cycle COz Incubator 5 3 Section 5 Routine Maintenance 5 4 Reverse Door Swing continued Steri Cycle COz Incubator 10 Locate the small cover plate securing the door cord Figure 5 3 Remove the Cover plate and bolt hole screws from the plate and disconnect with door connector the door cord from the connector behind the plate Figure 5 3 Cover Connector Remove the four screws securing the 9 door hinges to the unit Place the door on a padded surface to prevent scratches Remove the four screws securing the inner door hinges and the two Phillips screws located between the hinges See Figure 5 4 Retain the screws Remove the two screws securing the door strike and the four Phillips screws located on this side of the door Figure _ 5 4 Rein he screws Figure 5 4 Screw Locations Move the door strike to the opposite side Orient as Figure 5 5 Install the four nylon hole plugs into the holes on either side of the strike A Y Rotate the inner door 180 and secure the door hinges to the unit Install the two Phillips screws into the is holes between the hinges eu Figure 5 5 Locate the cord cover on the bottom of the outer Knob Orientation door See Figure 5 6 Remove the eight screws securing the cover arrange the cord toward the opposite side and rein
6. 8 2 Section 9 Electrical Schematics 9 1 Section 10 Warranty Information 10 1 Section 11 Declarations of Conformity 11 1 Thermo Scientific Steri Cycle CO Incubator vill Thermo Scientific Section 1 Installation and Start Up Chamber Gas Sample Port Control Panel Sterilization Cycle Button Power Switch See Section 1 Inner Door Leveling lt Legs 4 Door Heater See Section 5 Cable Figure 1 1 Steri Cycle Components Outer Door Reversible to opposite swing see Section 5 Inner Door Reversible to opposite swing see Section 5 Chamber Gas Sample Port Used for sampling chamber CO content using a FYRITE or similar instrument Main Power Switch Control Panel Keypad displays amp indicators Figure 1 2 Leveling Legs Used to level the unit Power Switch Mains disconnect Sterilization Cycle Button Switch to initiate sterilization cycle Note The incubators are stackable Information follows A Steri Cycle CO Incubator 1 1 Section 1 Installation and Start Up Control Panel Silence Mutes the audible alarm Components Alarm Indicator Light pulses on off during an alarm condition Mode Select Switch Used to select Run Setpoints Calibration and System Configuration Modes Message Center Displays system s
7. 1 1 Control Panel Components 1 2 Keypad Operation mua a 1 3 Displays idi oie aaa taa DET dd ua meted deci vede 1 4 Installing the Incubator 1 4 Stacking the Incubators loss usa 1 5 Preliminary Pel ursi mies 1 7 Installing the Shelves see tengo omic eae 1 7 Access Port Filter amp CO Sensor Cover Plate 1 8 Installing the Air Sample Filter 1 8 Installing the HEPA Filter ae sca 1 8 Leveling the pated ue ead aca ir 1 9 Connecting the Unit to Electrical Power 1 9 Filling the Humidity Pan 1 10 Connecting the Gas Supply 1 11 Incubator Start ne esed 1 12 Setting the Operating Temperature 1 12 Setting the Overtemp Setpoint 1 13 Setting the CO2 Setpoint 1 14 Calibration Peres uad mi sad sa EE LE a 2 1 Temperatures E aaa ER EY A 2 1 Thermal Conductivity CO System 2 2 Infrared CO2 System e vate e S esa a OC LP S 2 3 Relative Humidity sube S Aoi ee keh AR SEEN 2 4 Steri C
8. Description 190699 its Removable feather gasket 190670 Duct sheet and shelf channel kit L91650 Micro board main control 230135 Fuse TD 5 x 20mm accessory outlet 115V 230120 0 5A fuse TD 5 x 20mm accessory outlet 230V 156117 iA Motor 2 pole 115VAC 50 60 Hz 190609 eis CHEESE Display board 290184 Probe 2252 Ohm 25 C 1 8 x2 224166 anie t Copper shelf 224175 5 Lick wie a dre Stainless steel shelf 130077 sees tte Stopper with 3 8 hole 290168 Thermal conductivity T C type CO sensor 281204 Infrared type sensor 250087 valve kit with assorted fittings 231203 RH sensor assembly RH display option 230180 12A DPDT circuit breaker switch power 400119 Switcher 40W 12 5 12V 196018 34 position control to display ribbon cable 360213 Pressure switch Gas Guard option 25012 oou Gas valve Gas Guard option 190519 4 20mA output board 1905495 eee a 0 5V output board 100544 SR SS 0 1V output board 4201042 AG 1KVA transformer 350239 0 vc eee Inner door switch 3602 Outer door switch 360236 04 Lars t Sterilization initiation switch TOO T ave Sa ue diui ey a Blower wheel 1909851 Access port filter assembly 770001 Disposable filter 99 9
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10. 78 WISE REFERENCE CHART GA COLOR ND GA COLOR NO GA COLOR 79 1 16 BROW 21 18 RED 42 22 3 BLACK 80 2 16 BLUE 22 18 GREE 43 22 3 RED 81 3 16 GRN YEL 23 18 ORANGE 44 22 3 GREEN 4 16 24 18 GREE 45 22 BLACK 82 5 16 WHITE 25 22 BLUE 46 20 RED 93 6 18 BLAC 26 22 BLUE 47 20 RED 7 16 YELLOW 27 22 BROW 48 20 BLUE 84 8 18 ORANGE 28 22 BROW 4 20 BLLE 05 8 18 RED 29 22 YELLOW 50 20 ORANGE 0 18 BLUE 30 22 YELLOW 51 20 ORANGE 1 2 x L 86 18 RED 3l 22 RED 52 20 BLACK 87 2 32 22 RED 53 18 BROWN 99 3 33 2 GREE 54 24 4 BLACK 4 Sx 34 22 GRAY 55 24 4 WHITE 89 5 e ere 35 22 PURPLE 56 24 BLACK 90 6 36 22 ORANGE 57 24 4 RED K 7 18 ORANGE 37 22 3 BLACK 58 24 4 GREEN 81 8 18 BLACK 38 22 3 RED 59 g2 g YELLOW 39 22 3 WHITE 60 20 40 22 2 RED Q 93 41 2272 BLACK GA 95 55 97 98 99 00 01 02 03 04 05 MODEL DESCRIPTION 3541 STERI CYCLE TC 2 INC 230 06 3543 STERI CYCLE IR CO2 INC 230V 07 5 S1 9962 04 25 09 CCS CHG 190783 WHT DISPLAY 190608 BLU 4 IN 3759 01 22 07 GJG GJG LON ADDED DH 8000 SERIES MODELS 3 N 3124 08 04 03 JNL LUN CHANGED 290137 TEMP PROBE 290184 E ecvr ica Schenat 2 IN 2970 09 04 01 GLM LON CHG HARNESS FOR MICRO TD SENSE VOLTAGE LOSS 1 14 2915 04 02 01 GLM DN
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12. JO aq 9 1 JOSUBS 200 eu Ajuo sued Ie puooes e JO IM H1 4osu s 200 2U Jee euo poued jeu uonippe uj Joqe Bundeoxe su dx 5 je Jo diusueunpjow JO u BuruJojuoo uou o ue oJd sped 151 ou Buunq ponad Je eu Buunp saumo juenbesqns Aue spu x uono oid y si jueuudinbe 1 euin ewes y A jejeuurxoJdde p y AjueJue y os Buiddius siu Ajroej uno peddius si juswdinbs ejep eu SUJUOLU OM 5 AUeJeM 941 xALNVSSVM YOLVENONI 319A9 19915 2131133195 H3HSI3 Thermo Scientific Steri Cycle Incubator 10 2 Beclaration nf Conformity Manufacturer s Name Manufacturer s Address Product Description Product Designations Year of Initial Marking CE Affected Serial Numbers Thermo Fisher Scientific Ashevitle LLC 401 Millcreek Road Marietta Ohio 45750 U S A Steri Cycle Incubator 370 2001 Release 1 Release Level REL shown on Serial Tag This product conforrns to the following European Union Directive s EMC LVD 89 336 EEC 73 23 This product conforms to the following Ha
13. Gas Guard Operation continued Humidity Readout P N 1900091 Steri Cycle COz Incubator Audible and visual alarms occur on the control larm panel when the gas guard switches from one Indicator gt 777 supply to the other The audible alarm sounds Silence u until the operator presses the Silence key on Key the control panel The visual alarm in the ne Message Center reads Tank 1 Low while the audible alarm is sounding but the message is Figure 6 6 Alarm Response removed when the operator presses the Silence key However the Tank Low indicator on the control panel stays lighted until the condition is resolved The unit operates normally Caution Both the audible and visual alarms described above do not ring back after the Silence key is pressed If the Gas Guard system does not detect an adequate gas supply at CO2 Inlet 1 or Inlet 2 a visual and audible alarm again occurs on the control panel The visual alarm in the Message Center reads Tank 1 amp 2 Low The audible alarm continues to ring until the Silence key is pressed The audible alarm ring backs every 15 minutes after the alarm is silenced if the Gas Guard continues to detect that both gas supply pressures are below 10 psig 0 690 bars Model 370 380 Series incubators can be equipped with a humidity sensor to monitor the relative humidity inside the chamber The sensor is located on the chamber ceiling and provides a signal that is displayed in 1
14. Low CO Alarm Limit High CO Alarm Limit CO Alarm Relay Configure Mode Chart 3 1 Page 2 of 3 0210 Press to return to previous parameter Press to retur to previous para Press to return Scroll for Parameters M y eme CO2 HI Scroll for Parameters um CO2 RLY XXX Scroll for Parameters Section 3 Configuration Numbers increase Press Enter to save the setting Numbers decrease Numbers increase Press Enter to save the setting Numbers decrease CO2 alarm relay ON Press Enter to save the setting C02 alarm relay OFF to previous parameter Numbers increase Press Enter to T C CO Zero Number T C Z save the setting Scroll forParameters Numbers decrease Press to return Numbers increase Press Enter to save the setting T C Co Span Number Mode Press to return to previous parameter T C S Scroll for Parameters Numbers decrease f Y IH Numbers increase Press Enter to save the setting Scroll for Parameters RH Low Alarm Limit Mode Press to return to previous parameter Us Numbers decrease RH alarm relay ON Press Enter to save the setting RH alarm relay OFF RH Alarm Relay Press to return to previous parameter RH RLY XXX Scroll for Parameters 1 ey refer to next page Thermo Scientific Steri Cycle CO Incubator
15. lentitic Thermo Sc odel 370 371 380 and 381 Steri Cycle Incubator 370 200 9 D Rev 6 Page 1 of 2 8 8 Steri Cycle Incubator Section 8 Spare Parts 2 JO Z 9 G 6 6 002 0ZE Leg pue 082 LZE OLE sued 060 1028 71130 HOLIMS 8000 BNN 11 130 09470 IWS BONY 4000 83NNI TIVL30 OE Oz0pv2 18021115 3901 31445 OLE 3901 TANG pr 01 3001 123781 202273817741 cv 9 6510214 TANIA 3801 193 20917 3801 LOFNI ASSV 31714 JVI YANNI 1 10 000S0LE ATGNISSY TINVd 1081NO2 10093 10 00020 ATGNISSY dYYM 301831X3 1 10 0001016 ATGNASSY 139 WOVE 002 OLE 405835 T us vert ATGNISSY 331015 8000 YINNI 1 S1 CeEo061 1188355 8000 0183103 2 10 71 0061 401 801843113 1 16 8060061 ve WOVE 801931X3 1 18 1080061 INN 310H M JLV1d 8000 55719 1 91 2810061 9N01 9 41815 01431 MINIT 1804 9 S11081 13MSV9 38021115 YOOd YANNI 017801801 39021115 13 6 9 xv3ugiv3u 0167201601 62 INI OLE 801 012 8000 950061 82 WIL 180843 sieooe 12 A18N3SSV 8000 YANNI 9 8321038 ONIGAL X 9175 920078 831114 VId3H 3101504510 100011 8 YIHSVM 1 15 18021115 00 2 1 9800 L U3HSVM YIGINO
16. PRESS ENTER Thermo Scientific Remote Alarms Thermo Scientific Section6 Factory Options A description of the factory option connections to external equipment follows A set of relay contacts is provided to monitor alarms through an RJ11 telephone style connector on the back of the cabinet Refer to Figure 6 5 for the location of the alarm connector The 12 foot telephone cord P N 190388 and RJ11 to screw terminal conversion box P N 190392 are available through the Technical Services department The remote alarm provides a NO normally open output an NC normally closed output and COM common Refer to Figure 6 1 The contacts will trip on a power outage or an overtemperature condition The contacts may also be programmed to trip or not trip on temperature alarms alarms and RH alarms See Section 3 Configuration Mode NO green wire COM yellow wire INC ALAIIM CONTACT ALIS red wire SOV max at max not connected black wire Alarm Contacts Junction Box IMPORTANT USER INFORMATION Caution Stored product should be phone cable to incubator alarm contacts RJ 1i connector protected by an activated alarm system capable of initiating a timely response All outputs shown 24 hours day These alarms provide in alarm condition interconnect for centralized monitoring Figure 6 1 Remote Alarm Contacts CAUTION Contains Parts and Assemblies Susceptible t
17. 830 820 10 0177 V N NOTSTASA 141942530 099 OVI 48 3140 NJ3 A38 amp c 00c 0LE SAC Section 8 Spare Parts Thermo Scientific Steri Cycle CO Incubator 8 2 Section 8 Spare Parts 8 3 8 002 0LE 06457 DIHO vil3IavW 3 i F XX WWI 230 S3 ONY VIS OSEWIN SNTNVAC 031912995 38188810 5531 1 NATL JI4ILNAIDS ESE OSE OL NOWWOD 1 Jeusigouueu L n JISHINGTIS 396884 WAHL WOR MISSIN 0020 3705 10 9 p 310 AND Oddy 0 TWO NILA 04 350 SLL DAO MON 350800 ANY 803 5 01 035077510 38 QL LON SI NOLLYNADANI HITS ONY 3104 14315 531435 OLE WN 8 SNIVINI IN3WIDIC STIL 5 3801 3NDJITIS 020772 9 INOIITIS 2 2 NIV EI ANOSNAS IANLYJIAWIL 81062 e J NNYHIJ 47SHS 1 1 99061 11 901 DI331X3 1 1 80 0061 01 ATAWASSY ONS LING 531955 OLE 112006
18. Model 370 Series Steri Cycle Incubator Direct Heat with Sterilization Cycle Operating and Maintenance Manual 7000370 Rev 19 Preface Model Sensor Voltage 370 T C 115 371 T C 230 380 IR 115 381 IR 230 T C is a thermal conductivity sensor IR is an infra red sensor All units are 50 60 Hz MANUAL NUMBER 7000370 19 25248 1 3945 6 9 09 Updated drawer specs sliding and stationary CCS 18 24891 51 9962 6 2 09 Display board from 190793 to 190609 color updated 370 200 7 amp schematics CCS 17 24900 IN 3923 4 20 09 Updated door interlock switch 370 200 9 D CCS 16 25303 IN 3902 2 3 09 Updated decontamination issues for UL page 1 7 CCS 15 24975 11 3 08 Updated water specs on pages 1 10 and 1 11 CCS 14 24446 1 3848 4 22 08 Updated exploded parts drawings wingnuts to hex nuts white to grey silicone hole plug to snap bushing CCS 13 24344 IN 3824 1 16 08 Removed stud from back panel chg d ground wire placement updated all CAD drawings ccs 12 23 84 IN 3748 11 22 06 Note that vinyl on duct sheets not removed before shipping vinyl color chg CCS 11 23740 10 4 06 Replaced quarter turn fasteners with thumbscrews on top duct CCS 10 23451 IN 3294 6 30 06 Added 5 psig to incoming 2 pressure range CCS 9 10 21 04 Updated sterilization Checkpoints concerning stacked units D Figel CCS 224341 3211 8 25 04 Updated water qual
19. SI 9962 04 25 09 CCS CHG 190793 WHT DISPLAY 190808 BLU 5 IN 3753 01 15 07 GJG GJG LON ADDED MODEL NUMBERS 4 IN 3308 06 15 06 GJG GJG MSB ADDED MODEL NUMBERS 3 3124 08 04 03 JN LON CHANGED 290137 TEMP PROBE 290184 E ectr ical Schenat IC CE co eer cee RE ES Model er een m 370 and 380 We se htm e Heat Steril ization ATTENTION ER ANDA ak se PEP on Gy n eme one SE Incubotor OBSERVE PRECAUTIONS MATERIAL ELECTROSTATIC ThermoFisher PAINT COLOR 3 0 70 0 D REV 6 SENSITIVE DEVICES SCIENTIFIC BOX 649 MARIETTA OHIO 45750 TOLERANCE UNLESS OTHERWISE SPECIFIED XX t XXX 4 DRAWING NUMBER SIZE ANGLES DECIMAL 370 70 0 D Page 3 of 3 Thermo Scientific Steri Cycle COz Incubator 9 3 POWER CONNECTION 230VAC 50 60HZ 4 4 FLA s 42 POWER INLET RFI LINE FILTER 1 460180 9 POWER SWITCH CIRCUIT BREAKER 12 230180 j 4 EXTERIOR CONVIENCE OUTLET 4 44 0 54 FUSE 5 460138 IEL 320 230120 230 0 4 TRANSFORMER 1KVA 420104 15 7 IN
20. increments on the control panel The humidity readout can display continuously or toggle with the temperature readout In addition a low alarm limit can be set on the humidity readout to detect when the humidity pan runs dry Refer to Section 3 Configuration The sensor is capable of measuring relative humidity from 10 to 100 with an accuracy of 5 above 90 See Section 2 Calibration for details on calibrating the humidity readout Factors which may affect humidity levels in the chamber are the water level in the humidity pan frequency of door openings and the fact that the humidity pan is located on the bottom of the chamber versus on a shelf Caution Incubators equipped with a T C thermal conductivity sensor rely on a constant level of relative humidity to accurately measure and control the CO2 concentration in the incubator Thermo Scientific Thermo Scientific Section 7 Specifications Specifications are based on nominal voltages of 115V or 230V in ambients of 22 to 25 Temperature Control 0 1 C Microprocessor PID Control Setpoint Digital Touch pad 0 1 C Range 5 C above ambient to 50 C Uniformity 0 3 C 37 C Tracking alarm User programmable low indicator Overtemp Tracking user programmable action and indicator Display Digital LED 0 1 C increments Temperature Safety Type Extreme temperatu
21. 4 Press the Mode key to return to Run Mode Steri Cycle COz Incubator 3 1 Section 3 Configuration 3 2 Set REPLACE HEPA filter reminder Set Access Code Steri Cycle COz Incubator A HEPA filter replacement timer can be set for a specific amount of time from 1 to 12 months of actual unit running time Time will not accrue when the unit is turned off The default time is 6 months When the allotted time runs out REPLACE HEPA appears in the display and the visual alarm flashes To set the reminder use the following procedure 1 Press the Mode key until the Config indicator lights 2 Press the right arrow until REPL HEPA is displayed 3 Press the up down arrow to choose the number of months desired 4 Press Enter to save the number 5 Press the Mode key to return to Run Mode or right left to go to next previous parameter Note After the reminder has been set check the allotted time remaining by going to Config Mode then pressing the right arrow until NEW HEPA XXX displays This number is the remaining days before the filter replacement time specified runs out For example if 12 months was chosen in the REPL HEPA XX message screen the NEW HEPA number would be 365 days A 3 digit Access Code can be entered to avoid unauthorized personnel from changing the setpoints calibration or configuration A setting of 000 bypasses the access code The factory setting is 000 1 Press the Mode key until the Config indi
22. Incubator 1 11 Section 1 Installation and Start Up Connecting the Gas Supply cont Incubator Start Up Setting the Operating Temperature 1 12 Steri Cycle CO Incubator Warning If higher purity CO is desired inside the incubator greater than 99 596 pure the pressure regulator should be constructed with a stainless steel diaphragm along with specifying the purity of the CO from the gas supplier Follow the manufacturer s instructions to ensure proper and safe installation of the pressure regulator on the gas cylinder Consult your facility safety officer to ensure that the equipment is installed in accordance with the codes and regulations that are applicable in your area A The CO gas supply being connected should be industrial grade 99 596 pure and should not contain siphon tubes Install a two stage pressure regulator at the cylinder outlet The high pressure gauge at the tank should have 0 2000 psig range The low pressure gauge at the incubator inlet should have a 0 30 psig range Input pressure to the incubator must be maintained at 15 psig 103 4 kPa The incubator has serrated fittings on the back of the cabinet to connect the gas supply Refer to Figure 1 14 The fitting is labeled CO2 Inlet 1 Tank Make sure that the connections are secured with clamps Check all fittings for leaks For units having the CO Gas Guard option refer to Section 6 With the incubator properly installed and connected
23. 801 0351 5405835 202 231111 OMG 35048404 ANY 401 SYIHLO OL 035012510 38 01 LON 51 NOILYNYOANI HONS NV NOILIVNUOJNI 531825 OLE 3JWVN 18Vd 1300N AYVLIIYdOYd SNIVINOO 1N3N0200 SIHL A18N3S S V 405835 21 ANVIV S ALY 560614350 A18N3SSV YOSNAS 1 40 U38MVH 1NVIV3S SEOEI ALY 381 u31avov 1 1 1 52 0061 016 4015 Yl 000061 JOD usa 3801 Q3LNIOANOD 211992 INNOW 805835 81 S0cItc 1NV1V3S ALY 560614 350 WOSN3S 81 70212 13svo E001 QNIHSNG dVNS e 1 89006 MANIS SHIL 2 1 X 84 06072 HSVAYT dZ 25 84 20062 83825 SS W E X 22 88 61022 ONIY YILdVOY 21 I 1 92 0061 9818 dOLS IL 1260061 JLW1d 21 OLE 2510061 y 9NINIVUIS3U SMO1138 8110061 21 3NOj1 SA0d 2800061 0114182530 18 4 ON 18 4 805816 OL dWIL IH 991062 S1VI3431VN 30 1118 3801 031010AN00 ZIIZ H HSWMMT dZ 2 amp 10062 631135 0008 01 NOMAO2 0300Y 10 22 10 6518 1 Ha ss 067 ix 2 9 17172 310N 03007 90 06 90 9066 11 1 14 W3IV3H 2610051 03001 40595 21 10 12 60 1962 81 S30 L Vd 405835 21 01 SINN 7 ONIA 8110061 03001 10 90 10 5162 81 011200044 803 03513133 10 72 70 0 VN 40 1118 NOISIAIY 30 0114182 10 444 0 2 18 on 8 8 002 01 YIGWNN OMQ Steri Cycle CO Incubator
24. NI OLE 3WVN 1UVd 1300N AYVL91YdONd SNIVINOD 18380200 SIHL 1 913 0 1 YIHSYN 11 SLE 020078 82 1 9 11111 QHNTG INN SLE 107020078 12 1 ONILLIJ QW3HWING 052 2117020078 92 1 8 OLE 3901 LIINI TANIA 100S6 113M 2027016 S 1 9 ONIONL TANIA 9176 GIOZL 2 13N1 202 01 v2 1 TANVd YY 1 0061 t2 1 JUVNOS I SE1006 22 2 14 0 ONIGNL 26 6 900018 1 831113 VId3H 3181504510 100011 1 1 SLE UIHSVA 301 5 I 2167 83ddVNS 590009 GLE dNYT3 U3ddVNS 10009 081097 1 131100 83404 961097 131100 NI dVNS 20097 1 A21 1083105 590052 1 CAINO 08 101 30V1 1F 202 395022 139 1 68 58 595022 I 38V1 01 83338 069022 1 38V1 LOVINOD HBV IV 186022 6 1 1381 13100 SS 552022 9 1 LNNON 131110 480 5320V 166061 1 ANNO 18 ILE 310 9014 3108 NI dVNS 2 1 9 B B3HSVAM201 1001 113 9F 802 1 UIHSVA 1 14 SS 9 72062 KINO OBETOTRN HSVAYT 47 2 9 10062 9 83425 9 Y 2t 90 67022 i M3UDS dHd SS 2 2 9 11022 1 ALO 0114182 10 Lud ON nva ON SiVIg3IVN 40 1118 13 4 XOVG NONI 0015 03A0M3U OVS Lo 21 zi1 rzec ui 9 531815 008 Ol NOWNOD 03007 oro oro 10 22 10 6s2 N1 5 1t 310N 03009 oro oro 90 06 90 9026 v 025 OL JAWA 8 44112 590052 0 9 2 801 90 vo 61 so 1816 15 131100 Q3S1A38 wa
25. all visible signs of spills Odor may occur during the Sterilization Cycle and is considered normal The Steri Cycle is not intended to sterilize other items instruments etc from the lab During the cycle the unit chamber becomes hot enough to melt samples instruments dishes etc left inside the unit The items listed below also need to be removed 1 HEPA filter 2 Air sample filter 3 Access port filter assembly 4 Water in the humidity pan _ 5 Temp RH recorder probe if applicable 6 IR if applicabl IR sensor if applicable Access Filter S Air Sample Port Filter Filter During the Sterilization Cycle discoloration and tubing 1 of some materials may occur For example stainless steel turns a straw color after a period of exposure to high temperatures This is normal 5 8 Steri Cycle COz Incubator Thermo Scientific Section 5 Routine Maintenance Checkpoints What if Then Cycle does not initiate or terminates in mid cycle Check for alarms SYS IN AIR SNSR ERR TMP CTRL ERR Units are stacked A Sterilization Cycle performed on either unit affects performance on second unit Do not use second unit during Sterilization Cycle The Sterilization Cycle may be performed on both units simultaneously with proper electrical connection No action is taken within 1 minute following display prompt Unit returns to norm
26. periodically during the first week of returning the unit to service Caution The high temperature sterilization cycle may cause the T C CO sensor output to change significantly This is normal and does not indicate damage to the sensor Therefore it is essential that the CO2 calibration is verified before returning the unit to service If an independent instrument is not available to verify calibration the following procedure may be performed After the Sterilization Cycle and before CO is allowed to enter the cabinet 1 Fill and install the humidity pan 2 Allow to stabilize at the desired operating temperature for at least 12 hours 3 Calibrate the display to 0 0 Note If the Cool Phase is canceled the display toggles between CYC CANCELED and COOL PHASE With T C sensor When the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles between CYC CANCELED REPL HEPAs and PRESS ENTER The green LED is lighted but no longer flashes With IR CO2 sensor When the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles between CYC CANCELED POWER OFF and REPLACE IR The green LED is lighted but no longer flashes After the unit is powered back on with the IR sensor reinstalled the display toggles between CYC CANCELED REPL HEPAs and
27. the parts bag shipped with each incubator Figure 1 3 Stacking Brackets 1 Remove the cover plate securing the door cord from the incubator to be on top See Figure 1 4 Disconnect the plug from the connector Cover plate and bolt hole with door connector Figure 1 4 Cover Plate Figure 1 5 Hole Plug Locations 2 Remove the four screws securing the door hinges to the unit Remove the door and set it aside 3 Unscrew the two hole plugs from the top cover of the incubator to be the bottom of the stack Figure 1 5 4 Unscrew and remove the 4 leveling feet from the unit to be stacked on top and lift it onto the bottom unit Align all sides Warning This incubator weighs 260 lbs 118kg Have sufficient personnel available when lifting Lift the unit by the sides of the cabinet base to avoid placing stress on the outer door hinge Figure 1 6 Stacked Units Steri Cycle COz Incubator 1 5 Section 1 Installation and Start Up 1 6 Stacking the Incubators cont Steri Cycle COz Incubator Insert the stacking brackets into the slots at the back of the stacked units as shown in Figure 1 7 Align the slotted holes in the brackets with the mounting holes on the back of the top incubator Secure the brackets with the screws and washers provided in the parts bag See Figure 1 8 Top Incubator back lt s Unit u gt E or ui Figure 1 7 Bracket into Slot
28. to previous paramete 2 6 Steri Cycle COz Incubator Clart24cdr Thermo Scientific Turn Audible Alarm ON OFF New HEPA Filter Thermo Scientific Section 3 Configuration Several features available in the Configuration Mode allow custom setup of the incubator These features are listed with descriptions below All features may not be necessary in all applications but are available if needed To enter Configuration mode press the Mode key until the Config indicator lights Press the right and or left arrow until the appropriate parameter appears in the message center See Chart 3 1 for more detail The audible alarm can be turned on or off The factory setting is ON Press the Mode key until the Config indicator lights 2 Press the right arrow until AUDIBLE XXX displays in the message center 3 Press up down arrow to toggle AUDIBLE ON OFE 4 Press Enter to save the setting 5 Press the Mode key to return to run mode or right left to go to next previous parameter When the REPLACE HEPA reminder displays and the visual alarm flashes the specified time has elapsed and the HEPA filter should be replaced To clear the display and reset the timer after replacing the HEPA filter with a new one follow the steps below 1 Press the Mode key until the Config indicator lights 2 Press the right arrow until NEW HEPA displays in the message center 3 Press Enter to restart the timer and clear the REPLACE HEPA alarm
29. xd 1 LN CA 33 n POWER SUPPLY 18 lt lt 400119 400132 FOR e EE ote KIT 190779 in 04 ns ze Wl J354 2 40 1 2 J9 2 L2 246 28 4 DISPLAY BRO ASSY 190609 3 RIBBON CABLE 25 195018 Ai gt 26 J4 2 e 7 J4 3 e J4 4 Lum 44 5 2 J4 6 205 J4 7 al 2 J4 8 32 J4 9 33 4 J4 10 34 2 OPEN A 35 3 CLOSED 36 1 SVDC POWER INLET RFI LINE FILTER 460180 POWER SWITCH CIRCUIT BREAKER 124 230180 EXTERIOR CONVIENCE OUTLET 460024 TRANSFORMER LKVA 420104 INTERIOR FAN MOTOR 115 SO 60Hz 156117 COMPONENT COOLING FAN 900135 amp CONTROL T STAT COR 400151 OVERTEMP SAFETY MANUAL RESET Open on Rise 400150 CHAMBER HEATERS BACK 39 1 56W 132058 BOTTOM 28 11242 132059 LEFT 23 92W 132060 RIGHT 23 92W 132061 COLLAR HEATER 48 192 132062 DOOR HEATER 89 356 132057 STERILIZATION PROCESS INDICATION 02 SOLENOIO 12VDC 250085 DOOR SWITCH INNER 360239 Shown w door open STERILIZATION INITIATE BUTTON 360236 DOOR SWITCH OUTER Shown w door open 360230 TEMP PROBE 290184 2252 OHM 25 MODEL 370 02 SENSOR 290168 Electrica Model 370 and 380 Heat Sterilization Incubator Schematic 370 70 0 D REV 6 Page of 3 Thermo Scientific Steri Cycle COz Incubator 9 1 39 E
30. 1 6 SS33V 4600061 8 8 v l SALT VId3H 31815064510 LL 1 MALTS VId3H 00S G 6 1094 9 Yd ALIGINMH SIOZEC S OUST 82 STSHS ONI SLIVZZ Y e 133HS 1210 av TQUM E2061 QNIN 0 SNOIITIS E EIOIOI e e kr LAN SS 02 1 vOOEZ l ALO 14192530 19 4 ON m SvIedivW d TII8 S3133S 0008 SOS OL NOWADJ 03007 5 079 40 22 10 65 v 1 0300Y asn Or 579 80 0 90 SOEE NI 81062 Dl 8089 1 002 INF 0 06 0 velE NI 13345 1210 WO SVL 30 MOX dll O3A0Na NOT 902 820 10 71 50 2962 1 NOTLINGDSd 203 35V3 138 820 820 1071177 Y N 0 NUISIA38 30 NDIIdI33JS30 ddV OYJ 48 diva ON N23 33 Steri Cycle CO Incubator 002 0 A0 Thermo Scientific v 00C 0 05 lt V1l13I VW t Ma 35 C aS 0536 a ESE zySE ISE OSE OL NOWWOD 1 S INTINYA 031312395 3SIM33HID SS3 NI DNV3J UI 2IJILN3I2S JeusiJouueu 81 0 vr 310 JNO 620 00 TWO NNO NSS FULL ONO 18815 5318 OLE SAWN MOSNSS 3aniva3dWal OLE velO62 11 901 1 802006 OI ATAWASSY dV TS 1409 55327 2
31. 3 11 Section 3 Configuration Configure Mode Chart 3 1 Page 3 of 3 refer to previous page To Configure Display Temperature Display Humidity Tank Select Gas Guard 3 12 Steri Cycle CO Incubator Display temperature ON Scroll for Parameters Press e Display temperature OFF to previous parameter Display humidity ON Press Enter to save the setting Press to return to previous parameter Scroll for Parameters Display humidity OFF Tank 1 prime P Enter t wwsux SEL X Sus Press to return J to previous parameter Tank 2 prime Gas Guard ON Press to return aS Gas Guard OFF to previous parameter Numbers increase Press Enter to Ent RS 485 XX save the RS485 address Scroll for Parameters Press to return to previous parameter Numbers decrease Chart3 1 3 cdr Thermo Scientific Section 4 Alarms The Model 370 380 Series incubator alarm system is shown in the table below When an alarm is active the message appears in the LED message center Pressing Silence disables the audible alarm for the ringback period However the visual alarm continues until the incubator returns to a normal condition The alarms are momentary alarms only When an alarm condition occurs and then returns to normal the incubator automatically clears the alarm condition and the message center Y Table 4 1 Alarm and Description Description No alar
32. 370 Series incubators can be equipped with a built in Gas Guard system that will operate with a CO gas supply The Gas Guard uses two pressure switches to continuously monitor the pressures of two independent CO supplies and automatically switches from one supply to the other when the supply pressure drops below 10 psig 0 690 bar The Gas Guard s design does not facilitate use by multiple incubators The CO gas supplies must be equipped with two stage pressure regulators with gauges The high pressure gauge at the tank should have a 0 2000 psig range and the low pressure gauge should have a 0 30 psig range The gas supply to the incubator must be maintained at 15 psig 1 034 bar 5 psig Gas pressures below 15 psig will cause nuisance alarms to occur on incubators equipped with the built in Gas Guard 6 4 Steri Cycle 602 Incubator Thermo Scientific Connecting Gas Supplies De activating Gas Guard Gas Guard Operation Thermo Scientific Section 6 Factory Options The inlets for the Gas Guard are located on the rear of the cabinet Using 1 4 ID tubing connect one of the CO supply tanks to the fitting labeled CO2 Inlet 1 Tank Connect the second CO supply tank to the fitting labeled CO2 Inlet 2 Tank Install 3 8 hose clamps to secure the 1 4 ID tubing to the fittings on the rear of the drawer See Figure 6 5 Optional RS 485 002 Inlet r 2 Tank Accessory Outlet 75 watts max r
33. 6006 5 31vld 001 55012 8 1 74 T33HM 3 078 1110061 L SaldvOY Nv3 SSYAB E800061 9 B3HSVM NOT331 _ 21006 S O3ONVH 1337 SS 02 7 1 5 0019 y e Oc v l 33JVdS OSc C 990 21 E M HM 511001 2 y MIN ONIA SS 160 2 ALO 119149530 Lavd ON 1avd N S VIa3IVN 3D 1118 531435 0008 01 03007 5 979 979 40 22 10 BSLE NI 2 0300Y GSW SCD 90 0 90 I NOILINGDsd A03 03Sv3133 ANG 820 890 10 11 7 Y N 0 NOISIA33 JU NDIIdI33JS30 OVI 48 310 UN N23 A38 G 7 D02 OLE S38 IMO Section 8 Spare Parts Thermo Scientific Steri Cycle CO Incubator 84 Section 8 Spare Parts 8 3215 UIBNNN ONIMVYO 3 E 5 002 OLE XX 230 031112145 JISIMYJHIO SSIINN 1 NVH1701 S319NV VIN INIVd seo 3woS 179276 aaa 820 ava OSLSY OIHO VLI3IBVN 699 21411 31265 40ySijOULIOUL 21311N313S 834511 ONUJHI NOW NOISSINU3d N3LLIUA 8 5 002 016 U38NQN OMG ANOHJIM 3504804 ONIUDIOVJQNVW 803 350 A19W3SSV T3NYd 32 8 OLE 31111 ONQ YON 3504804 ANY 03 SYIHLO Ol 415012510 38 01 LON 51 NOILVNSOJNI HONS ONY NOI LYAHO
34. 7 CO line air sample 760175 HEPA filter 5 5 diameter 760199 HEPA VOC Filter Replacement Kit 760200 HEPA 117036 silicone plug 101018 O ring 1900094 HEPA VOC Filter Replacement Kit 760200 HEPA 190985 access port filter 770001 filter Thermo Scientific Steri Cycle COz Incubator 8 1 8 lt 062 F XXX OS Sb OIHO V1LJIAYW X08 T XX WNIJ30 S3 NY TAM QEN 09112349 3SINS3HIU SSIM 32948371 JI4ILNAIDS EVGE ZVGE VSE OVGE 01 NDNND3 JLH INIA Jeusi 4 sro tog ava pa 31007 18315 531935 OLE WN Leva SSHSVM 2 115 OLE 6900 4 01 901 01831 3 1 16 80 0061 6 3D lavd OLE 5 AMDT Eld 006071 8 ONIHSNA 14 5 _ 51600 4 INI 20 04 200061 9 33HSVA NOWAL 4 0082 f S A333S SHL SS B E X 2E Ol 900055 v SSWAOASNVAL VAX 1 olioz lVISOWB3HL 061007 2 SOLOW AMO 411961 0119187530 Lave ON E SVISdIVN 30 1119 002 0 NO NNDHS HOG 150 2 O3ADW3Y S23 OVS INF 80 11 0 531935 0008 804 01 NOWNOD 0300Y SSW 9 9 OCS 0 22 10 6SZENI ALON 03007 GSW 979 9 9 90 0 90 SDEE NI NOTLINGOdd 803 3573793 JNO
35. BS 709 Jee euo poued uenem u uonippe uj oqe su dx s ouueu je Jo diysuewyJom JO u Buluuojuoo uou eq o ue oJd sued 5 Buunq poued ysi eu Buunp juenbesqns Aue o uonoejoud uenem y p J AI p si jueuudinbe noA ewes y AjueJuew eui OS Buiddius siu peddius si jueuudinbe ino ejep SY M OM 5 AjueueM Su L VSN YOLVENONI 319A9 14315 91311343195 YAHSIS 10 1 Steri Cycle 02 Incubator Thermo Scientific 60 r 9M L006 5 10 220 1963009 WSN episino uoneoidde 5 pue s s uoneJedo AjueJiew jueuudinbe uo suonsenb noA Apea 94 9M E9 P ELE 0 epeueSO pue VSN 008 1e sadas eoiuuoe p nb s eo n es jueuudinbo J AlJU A 1d pue uonejejsur uononu su paud SeAuJe jueuudinbe noA uoneJedeJd BAISUBYSJGWOD uM
36. C i Locate the opening in the top left corner on the inside of the chamber Remove the tape covering the opening on the outside of the unit Locate the stopper with filter in the hardware bag Lift the metal port cover and install the assembly in the opening inside the chamber See Figure 1 12 Also in the hardware bag is the CO sensor cover plate Install using the thumbscrews Refer to Figure 1 12 for the location e Thumbscrews Access Port Filter Air Sample and stopper Filter assembly J Figure 12 Filter and Plate Installing the Air Sample Filter Remove the filter from the shipping bag Install the air sample filter assembly to the black hose barb behind the top duct Insert the other end of the filter assembly onto the metal tubing on the top duct Refer to Figure 1 13 Installing the HEPA Caution Be careful when handling the filter The media can be damaged if Filter it is mishandled To avoid damage to the incubator do not operate the unit without the HEPA filter in place 1 8 Steri Cycle COz Incubator Thermo Scientific Installing the HEPA Filter continued Leveling the Unit Connecting the Unit to Electrical Power Thermo Scientific Section 1 Installation and Start Up 1 Remove the filter from the shipping box Remove the plastic coating from the
37. F CHG 130825 FAN 900135 amp REV FLA Mode D 1 23 01 Gy Gy DNF RELEASED FOR PRODUCTION 1 REV DATE BY CAD APPD DESCRIPTION OF REVISION 37 and 38 FREUT Heat Sterilizotion LI HI Y PU G E ATTENTION retain Dm m nef em D FT OBSERVE PRECAUTIONS 2 MATERIAL a ELECTROSTATIC ThermoFisher PAINT COLOR 371 70 0 D REV 5 SCIENTIFIC TOLERANCE UNLESS OTHERWISE SPECIFIED DRAWING NUMBER SIZE Dy 3 Ei 3 SENSITIVE DEVICES BOX 649 MARIETTA OHIO 45750 ANGLES DECIMAL oot 371 70 0 D age p Thermo Scientific Steri Cycle CO Incubator 9 6 E SERES DERI L006 OSI 60 7 eH uoneuuojul Jojnqujsip Jno 19ejuoo YSN eui episino suon eoidde eioeds pue 5 uonejedo Ajueuew jueuudinbe uo suonsenb noA JeMsue Apes 949M 9 OvZ epeue2 pue VSN 008 Je jueumnedeg sanag eoiuuooe INOA eseejd p nb s eores jueuudinbo J pue uoneJedo jueuudinbe SeAuJe jueuudinbe uoneuuojui djay si eoo 220 INOA Ssjonpoud 550 510104 1soj o
38. HS NO13J1 CLOOEL 00 LOP X 01 882 YJHSVA JAVM W3uSv 1v14 SS 01682 950061 0 LAN 9813201 201 313 SS 2 8 610019 6 83825 GH SShul 8 1 1 x 66005 8 83425 SdITIIHd GH SSQUI VIE X 2 9 m 3595 NOTAN 2 1 X 02 7 1 060066 M3U2S SHL SS B E X 26 018 900056 83425 SS dHd 2 1 0 920067 HOLIMS 4201831NI 62092 020 26 11 82 45 usur 117130 BONY HOIH _ 21002 1H913H JONIH 69 SS 60191 oi 55 19 14 31 MHL Z x sie 92 x oz 99060 6 13MSV9 39NIH 8000 YINNI EN ONIHSNG dVNS 2 1 SS UJHSVM 4201 IVNYJINI 9 UJHSVM 1V1J SS 88 3995 dHd SS X 2 98 345 SS B E X 26 98 _ 26022 3825 SS v x 2 98 16022 2 3825 IN quj SS P E 0251 _ 96002 ON 0114182530 1UVd ON 1998 KQ SIVIYJLYW JO 1119 8 9 Steri Cycle COz Incubator Thermo Scientific FW 09 DN WY POWER CONNECTION 115VAC 50 60HZ 9 6 FLA d 2 0 3 6 5 FUSE 5 230135 yg J TE 10 seal 5 6 J3 E Ra
39. ITIT eS f s O Power Cord Remote 002 Inlet Air Connector Alarm Contacts 4 Tank Intake 30V max 1A max RJ II telephone style connectors Used with the optional Gas Guard System R CO units only Figure 1 14 Back of Cabinet Fill the humidity pan with sterile distilled water to within 1 2 of the top Place the pan directly in the center of the incubator floor For applications requiring high humidity the pan should be placed against the left side wall of the incubator The side ducts have been modified to allow the pan to be placed against the wall Optimum humidity is achieved by capping the CO sample port This will however cause condensation in the chamber To enhance RH recovery from door openings place a second humidity pan in the right side duct For best operation of the incubator sterilized distilled demineralized or de ionized water should be used in the humidity pan Water purity should be in the resistance range of 50K to 1M Ohm cm or a conductivity range of 20 0 to 1 0 uS cm Refer to ASTM Standard D5391 93 or D4195 88 for measuring water purity Distillation systems as well as some types of reverse osmosis water purity systems can produce water in the quality range specified Tap water is not recommended as it may contain chlorine which can deteriorate the stainless steel Tap water may also hav
40. O2 The short term exposure limit for 15 minutes or less is 30 000 ppm 3 CO2 Carbon dioxide monitors are recommended for confined areas where concentrations of carbon dioxide gas can accumulate Warning This incubator is designed to be operated with CO gas only Connecting a flammable or toxic gas can result in a hazardous condition Gases other than should not be connected to this equipment CO2 gas cylinders have a UN1013 label on the cylinder and are equipped with a CGA 320 outlet valve Check the gas cylinder for the proper identification labels The gas supply being connected to the incubator should be industrial grade 99 5 pure Do not use CO2 gas cylinders equipped with siphon tubes A siphon tube is used to extract liquid CO2 from the cylinder which can damage the pressure regulator Consult with your gas supplier to ensure that the CO cylinder does not contain a siphon tube Gas cylinders should also be secured to a wall or other stationary object to prevent them from tipping A two stage CO2 pressure regulator is required to be installed on the outlet valve of the gas cylinder Input pressure to the incubator must be maintained at 15 psig 103 4 kPa 5 psig for proper performance of the CO control system A single stage CO pressure regulator will not maintain 15 psig 103 4 kPa to the incubator as the pressure in the CO2 cylinder decreases therefore a two stage regulator is recommended Steri Cycle CO
41. Press Enter to save the setting 5 Press the Mode key to return to Run or the right left arrow key to go to next previous parameter Steri Cycle CO Incubator 3 3 Section 3 Configuration Set Low Alarm The low CO alarm limit is the deviation from the CO setpoint which imi i will cause a low alarm The setpoint is variable from 0 5 Limit tracking alarm below setpoint to 5 0 CO below setpoint The factory setting is 1 0 CO below setpoint A minus sign in the display indicates that the alarm setting is below the setpoint 1 Press the Mode key until the Config indicator lights 2 Press the right arrow until CO2 LO X X displays in the message center 3 Press up down arrow to change the low CO alarm limit 4 Press Enter to save the low alarm limit 5 Press the Mode key to return to Run mode or right left to go to next previous parameter Set High Alarm The high CO alarm limit is the deviation from the CO setpoint which deat lac Limit tracking i ee above setpoint 1 Press the Mode key until the Config indicator lights 2 Press the right arrow until CO2 HI X X displays in the message center 3 Press up down arrow to change the high CO alarm limit 4 Press Enter to save the high CO2 alarm limit 5 Press the Mode key to return to run mode or right left to go to next previous parameter 3 4 Steri Cycle COz Incubator Thermo S
42. Serial Numbers Release 1 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s 89 336 EEC LVD 73 23 EEC This product conforms to the following Harmonized International and Nationa Standards LVD EN 61326 1 1997 EN 61010 1 1993 EN 50081 1 92 Amendments 1 and 2 EN 50082 1 97 EN 61010 2 010 CSA C22 2 No 1010 1 UL 61010A 1 5204 Ma Richard L Miller Regulatory Compliance Manager ThermoFisher SCIENTIFIC 16 April 2007 Rev 2 Aleclratinn of Contormity Manufacturer s Name Thermo Fisher Scientific Asheville LLC Manufacturer s Address 401 Millcreek Road Marietta Ohio 45750 U S A Product Description Steri Cycle Incubator Product Designations 381 Year of Initial Marking 2001 Affected Serial Numbers Release 1 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s 89 336 EEC LVD 73 23 EEC This product conforms to the following Harmonized International and National Standards EMC LVD EN 61326 1 1997 EN 61010 1 1993 EN 50081 1 92 Amendments 1 and 2 EN 50082 1 97 EN 61010 2 010 CSA C22 2 No 1010 1 UL 61010A 1 ZZ lt C z Richard L Miller CQE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 16 April 2007 Rev 2 Steri Cycle CO Incubator 10 3 Thermo Scientific Controlled Environment Equipment 401 Millcreek Road Mariett
43. TERILIZATION PHASE starts again when the chamber temperature reaches 140 C CANCELED COOL PHASE starts if the chamber temp dropped below 139 C For further information on CANCELED COOL PHASE see page 5 9 Thermo Scientific Steri Cycle COz Incubator 5 9 Section 5 Routine Maintenance 5 10 Sterilization Cycle Steri Cycle Incubator Warning The Sterilization Cycle heats the incubator interior surfaces to 140 C Contact with any surface inside the outer door during this cycle may result in burns 1 Empty the water from the humidity pan and place the pan back into the incubator Remove any samples instruments dishes etc from the chamber 2 Press and hold the large green sterilization cycle button on the right side of the unit Figure 5 10 for approximately 3 seconds until the LED lights Sterilization Cycle Button Figure 5 10 Sterilization Button Location 3 Enter the access code if applicable An access code is recommended to prevent accidental cycle initiation 4 Pre Sterilization with T C CO sensor The display toggles between REMOVE 5 REMOVE WATER and PRESS ENTER If Enter is not pressed within 1 minute the display returns to SYSTEM OR 5 Remove the HEPA filter air sample filter and access port filter assembly Figure 5 11 Tubing can remain in the unit during the sterilization cycle In addition remove the temp RH recorder probe and IR sensor if applica
44. TERIOR FAN MOTOR LISVAC 50 60Hz 156117 8 l 4 COMPONENT COOLING FAN 900135 d amp CONTROL T STAT COR 400151 o OVERTEMP SAFETY MANUAL RESET Open on Rise 400150 0 CHAMBER HEATERS BACK 39 156W 132058 BOTTOM 28 1120 gt l LEFT 23 924 132 RIGHT 23 92W 132061 2 5 COLLAR HEATER 48 1920 132052 3 D pe d 2 d DOOR HEATER 89 3550 4 132057 4 5 6 3 1 6 R 2 F2 17 0 LN 472 5 J3 3 POWER SUPPLY Z 20 400119 m BA 8 LAN STERILIZATION PROCESS INDICATION 400132 FOR Ca KIT 190779 21 04 O 55 E CO2 SULENDID 12700 i amp amp mE 250085 Wu 5 pa 22 Y 13 8 joi 23 226 25 2 23 J9 3 24 246 5 4 DISPLAY BRD ASSY 190609 34 27 RIBBON CABLE 5 es 0008 SWITCH INNER 360239 196018 Shown w door open 28 J4 2 5 29 LU L STERILIZATION INITIATE BUTTON 360236 30 J4 4 2 3 5 426 DOOR SWITCH OUTER 1 Shown w door open 360230 32 J4 6 33 4 7 31 TEMP PROBE 2252 OHMS 25C 290184 34 J4 8 32 Electrical Schematic 35 J4 9 4 COM J4 10 4 2 OPEN 371 and 38 MODEL 371 m ipa 37 5 S SENSOR Heat Sterilization 290168 Incubator 38 5 SVDC E N 371 70 0 0 REV 5 Poge 1 of 3 9 4 Steri Cycle CO Incubator Thermo Scientific Xe GOD DEP Ts COs BD cops BS Rea 26202 EO cle sO
45. a won 10 62 00 620 14 2 NOISIA3U 30 0119182530 ag ON 2PGE IvGE OpGE 8666 01 NOWHOD 71 8 5 Steri Cycle COz Incubator lentitic Thermo Sc F 4 0616 OIHO vlldluvA 679 FN SH TONY 31312345 1513410 553180 3328783101 DI4ILNAIDS J usijouusu L BYOLVENONT 31435 OLE INN 1tYc 13008 SNIYINOO 1N3W0200 STHL S d 3399 LNAOW 11083 1 0313 1007 4314 0 193 8 1 udldvdy 14 8 HOLIMS t 0810227 A TENISSY d 609061 262061 SOE G02 3925 dHd SS B E X 9 702 141425 AC 118 609061 Ol AVIdSIG LHM 61061 9H2 S SINN 4112 550019 2 6187 9 11180 1 061 00020 6 92098415 Ol 92680904415 1215402 1 14 NISN 03141004 38 Ol 13738 310N 8 1 002 018 2818 OMG Thermo Scientific Section 8 Spare Parts Steri Cycle COz Incubator 8 6 Section 8 Spare Parts 8 7 Tunt OSLSP OIHO V11318VM 69 9 00 01 TY INN ENSE APSE ISE OSE BESE OLNONMOO I YIBNNN 9NIMVUG 031312145 3S51MH3HIO SS3TN0 32NY83101 DI4dILNAIDS VIN C1NIVd Jeusijouueu L 00670 25 10 12 31VQ NOT QddV 820 0v2 31 0 NAG INADH 988890 OOH MOY 80 MWIN NOL RIA INOHLIM 1504804 ONIYNLIVINNYW
46. a Ohio 45750 United States www thermofisher com
47. al operation SYSTEM OK Need to cancel cycle in progress Hold down green cycle initiation button for 3 seconds Water is not removed from humidity pan during cycle Steam is produced and may cause burns Listed components are not removed before initiating cycle Components cannot withstand the sterilization cycle temperatures and are destroyed Unit is not powered off before removing the I R sensor if applicable Damage to the sensor may result Dummy 1 sensor is not installed The sensor cable cannot withstand sterilization cycle temperatures and is destroyed properly The outer door is opened during the heat or sterilization phases An outer door alarm occurs CLOSE DOOR in the display plus an audible cannot be silenced and visual alarm The outer door is open longer than 20 seconds during phases listed above Cycle is canceled unit goes to CANCELED COOL PHASE The outer door is open during the cool down phase when the temp is 60 C or greater An outer door alarm occurs Power interruption during the HEAT PHASE HEAT PHASE resumes if the chamber temperature was less than 90 C when power was interrupted HEAT PHASE resumes if the chamber temperature was greater than 90 C when power was interrupted and the temperature dropped less than 1 C CANCELED COOL PHASE starts if the chamber temp drop is more than 1 C Power interruption during the STERILIZATION PHASE HEAT PHASE begins if the chamber temperature has not dropped below 139 C S
48. ate to within 5 Thermo Scientific Steri Cycle COz Incubator 2 5 Section 2 Calibration Chart 2 1 Calibrate Mode Press MODE to light CAL 777 Re ET Silence Mode CALIBRATE Enter Inject CO 2 Scroll for Parameters C 4 n 9 To Calibrate a Numbers increase 0 Press MODE to move 1 Bises perating to SYS CONFIG sa hielo Temperature mode save the setting Press to return lt d Numbers decrease to previous parameter I 7 Numbers Increase Press Enter to T C CO Mode CO2CAL Emer save the setting when Thermal Conductivity sensor Is in use Press return to previous paramete Scroll for Param eters Numbers decrease pw t Numbers Increase IR Press Enter to 2 IR CAL XXX Enter when IR sensor is in use Scroll for Parameters save the setting Press to return lt Numbers decrease to previous paramete f Numbers increase D Percent RH EM RH o X3 ae D when RH option is in use SLE TEE save the setting Press to return lt Numbers decrease
49. atible w Model 1535 Alarm Monitor only Certifications Refer to the Declaration of Conformity at the end of this manual Thermo Scientific Section 7 Specifications 7 4 Steri Cycle 602 Incubator Safety Specifications Indoor Use Only ya bus pasa SE 2 000 meters Temperature 5 C to 40 C Humidity 80 RH at or below 31 C decreasing linearly to 50 RH at 40 C Mains Supply Fluctuations Mains supply voltage fluctuations not to exceed 10 of nominal voltage Installation Category II Pollution Degree 22 Class of Equipment N Installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laborato ries the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply Pollution Degree describes the amount of conductive pollution present in the operat ing environment Pollution degree 2 assumes that normally only non conductive pollu tion such as dust occurs with the exception of occasional conductivity caused by con densation Thermo Scientific Section 8 Spare Parts Part
50. ay toggles between POWER OFF and REPLACE IR When the unit is powered back on with the IR sensor reinstalled the display returns to SYSTEM Run Set Cal Contig cacacaca Silence Mode REMOVE HEPAS aD U Run Set Cal Config ZZ7 Silence PRESS ENTER 2 Run Set Cal Config lt REMOVE WATER P Figure 5 14 Displays with IR Sensor Removed Steri Cycle 02 Incubator 5 11 Section 5 Routine Maintenance Sterilization Cycle continued 5 12 Steri Cycle CO Incubator 6 After ENTER is pressed the Heat Phase initiates The green LED flashes and the display toggles between STERILIZING and HEAT PHASE During this period the HEAT light will be on and the cabinet heats to sterilization temperature Run Set Cal Config flashing Run Set Cal Config Sco es ode HEAT PHASE Figure 5 15 Heat Phase 27 Silence flashing 7 When the air temperature in the unit reaches 140 0 C the Sterilization Phase begins and the display changes to STERILIZING Run Set Cal Config Heat Temp 777 Ss oe m 140 Silence Mode STERILIZING mea 1 ele Figure 5 16 Sterilization Phase 8 After approximately 2 hours a 5 second audible ton
51. ble Discard the filters Temp RH A recorder probe N sensor HEPA ib plate Filter Access f Temp sensor Port Filter Air Sample inserted into and stopper Filter HEPA collar from assembly behind top duct Figure 5 10 Filter Locations Pre Sterilization with IR CO2 sensor If an IR sensor is connected the display toggles between POWER OFF and REMOVE IR If power is not turned off within 1 minute the display returns to SYSTEM OK Thermo Scientific Sterilization Cycle Thermo Scientific continued Section 5 Routine Maintenance Note To remove the IR sensor if applicable first power the unit off Then turn the two thumbscrews on the top duct until the cover plate disengages Secured on the inside of the plate is a dummy sensor Figure 5 12 on inside of plate A Figure 5 12 Dummy Sensor Figure 5 13 IR Sensor with Cable Grip the IR sensor and carefully pull it downward Unscrew the cable from IR sensor Figure 5 13 Set the sensor aside See Step 9 for the IR sensor disinfection procedure Connect the dummy sensor to the cable Fit the installed dummy sensor up into the previous sensor location When the power is turned on with the IR sensor removed the display toggles between REMOVE HEPAs REMOVE WATER and PRESS ENTER If ENTER is not pressed within 1 minute the displ
52. cator lights 2 Press the right arrow until ACC CODE XXX displays in the message center 3 Press up down arrow to change the access code 4 Press Enter to save the access code 5 Press the Mode key to return to the Run mode or right left to go to next previous parameter Thermo Scientific Set Low Temp Alarm Limit tracking alarm Enable Low Temp Alarm to Trip Contacts Thermo Scientific Section 3 Configuration The low temp alarm limit is the deviation from the temperature setpoint which causes a low temp alarm The low temp alarm is variable from 0 5 below setpoint to 5 below setpoint The factory setting is 1 below setpoint A minus sign in the display indicates that the alarm setting is below the setpoint 1 Press the Mode key until the Config indicator lights 2 Press the right arrow until TEMP LO X X displays in the message center 3 Press up down arrow to change the low temp alarm limit 4 Press Enter to save the low temp alarm limit 5 Press the Mode key to return to Run mode or right left to go to next previous parameter The low temperature alarm can be programmed to trip the remote alarm contacts A setting of ON allows this an OFF setting blocks the low temp alarm from tripping the contacts The factory setting is ON 1 Press the Mode key until the Config indicator lights 2 Press the right arrow until TMP RLY XXX displays 3 Press the up down key to toggle the setting ON OFE 4
53. ccess the only replaceable fuse in the incubator Thermo Scientific 1 Turn off the incubators power switch and unplug the power cord 2 Remove the two screws from the top of the control panel Grasp the control panel on each side and pull straight outward 4 Figure 5 8 shows the location of the fuse See Table 5 1 following for replacement fuse specifications 5 Secure the control panel and return the unit to service If the fuse blows after restoring power to the incubator contact the Technical Services Department Table5 1 Fuse Replacement Chart Fuse Voltage Manufacturers P N Amperage Rating Rupture Speed IEC Letter Code 115V ACC BUSS GMC 1 0A 1 0 Amp Time Lag T 230VAC ACC 8055 BK GMC 500mA 0 5 Amp Time Lag T Steri Cycle CO Incubator 5 5 Section 5 Routine Maintenance Replace Air Sample 1 Connect one end of the air sample filter to the hose barb on the Filter chamber ceiling 2 Connect the other end of the filter to the metal tubing on the HEPA filter adapter Figure 5 8 Air Sample Filter Figure 5 8 Connect to Metal Tubing Electronics Section 1 Turn off the incubators power switch and unplug the power cord 2 Remove the three screws from the back of the cabinet top 3 Slide the top backward about an inch and lift it off 5 6 Steri Cycle COz Incubator Thermo Scientific Section 5 Routine Maintenance Major Components Refer to Figure 5 9 Some components shown are factory installed op
54. cess port 1 1 4 inch 32mm removable silicone plug COP inlet eee 1 4 6 4mm barbed Sample port Front mounted barbed Electrical 115 Volt models 115VAC 50 60 Hz 1PH 9 6 FLA Operating range 90 125VAC 230 Volt models 230VAC 50 60 Hz 1 PH 4 4 FLA Operating Range 180 250VAC Power switch circuit breaker 2 Pole 12 0 Amp Accessory outlet Voltage equal to the cabinet input 75 Watts maximum 0 5ma leakage current Alarm contacts Deviation of temperature CO2 power NO and NC Thermo Scientific Section 7 Specifications Dimensions Exterior 26 1 W x 39 5 H x 25 0 F B 66 100 63 5 Interior 21 3 W x 26 8 H x 20 0 e a a 54 0cm x 68 1cm x 50 8cm Weight NE Ged s 260 Ibs 118kg Net shipping 315 165 143kg Copper Options Perforated shelf Customer Installed P N 224166 solid copper Humidity pan Customer Installed P N 237015 solid copper Copper interior components kit Factory installed Replace stainless steel duct work shelves and humidity pan with solid copper P N 1900095 Optional Data Outputs Analog Factory installed Stock 190544 0 1 volt Analog Factory installed Stock 190543 0 5 volt Analog Factory installed Stock no 190512 4 20mA Digital Factory installed Stock 1900085 RS 485 Comp
55. cientific Section 3 Configuration Enable Alarms to High and Low CO alarms can be programmed to trip the remote alarm Trip Co ntacts contacts A setting of ON allows this a setting of OFF blocks alarms from tripping the contacts The factory setting is ON 1 2 Press the Mode key until the Config indicator lights Press the right arrow until CO2 RLY XXX displays in the message center Press up down arrow to toggle the setting ON OFF Press Enter to save the setting Press the Mode key to return to Run Mode or right left to go to next previous parameter Set New Zero Number Ifa new T C sensor is being installed the two numbers on the for T C CO Sensors factory installed sticker on the T C cell must be entered to calibrate in the unit Note For the technician s convenience a label containing the two numbers on the T C cell is affixed inside the electronics drawer A Thermo Scientific Press the Mode key until the Config indicator lights Press the right arrow until T CZ XXXX displays in the message center Press up down arrow to change the zero number to match the sticker Press Enter to save the setting Press the Mode key to return to Run mode or right left to go to next previous parameter Steri Cycle CO Incubator 3 5 Section 3 Configuration Set New Span Number Ifa new T C sensor is being installed the two numbers on the 5 for T C Sensors factory installed stick
56. de you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over the telephone without a service call When more extensive service is necessary we will assist you with direct factory trained technicians or a qualified service organization for on the spot repair If your service need is covered by the warranty we will arrange for the unit to be repaired at our expense and to your satisfaction Regardless of your needs our professional telephone technicians are available to assist you Monday through Friday from 8 00 a m to 6 00 p m Eastern Time Please contact us by telephone or fax If you wish to write our mailing address is Thermo Scientific Controlled Environment Equipment 401 Millcreek Road Box 649 Marietta OH 45750 International customers please contact your local Thermo Scientific distributor Steri Cycle CO Incubator Thermo Scientific Preface Warranty Notes Information You Should Know Before Requesting Warranty Service Locate the model and serial numbers A serial tag is located on the unit itself For equipment service or maintenance or with technical or special application inquiries contact T
57. e Over Temperature Press to return to previous parameter Numbers increase Press Enter Percent C0 Mode to save setting C02 XXX Scmllfor Parameters Press return Numbers decrease to previous parameter Steri Cycle CO Incubator 1 15 Section 2 Calibration After the unit has stabilized several different systems can be calibrated In the calibration mode the air temperature CO2 and RH levels can be calibrated to reference instruments To access the calibration mode press the Mode key until the Cal indicator lights Press the right and or left arrow until the appropriate parameter appears in the message center See Chart 2 1 at the end of this section for more detail Calibration frequency is dependent on use ambient conditions and accuracy required A good laboratory practice would require at least an annual calibration check On new installations all parameters should be checked after the stabilization period Prior to calibration the user should be aware of the following system functions While the unit is in the calibration mode all system control functions are stopped so the unit remains stable Readout of the system being calibrated will appear on the message center If no keys are pressed for approximately five minutes while in calibration mode the system will reset to Run mode so control functions are reactivated Caution Before making any calibration or adjustments to the unit it is imperative tha
58. e Mode key until the Config indicator lights 2 Press the right arrow until RH RLY XXX displays in the message center 3 Press up down arrow to toggle the setting ON OFF 4 Press Enter to save the setting 5 Press the Mode key to return to Run mode or right left to go to next previous parameter Enabling Temp RH to On units equipped with the RH option the upper seven segment display be Displaye d the control panel can be configured to display Temp continuously RH continuously or toggle between Temp and RH If the units does not have RH the upper display always displays temperature If temperature is set to ON and the RH is set OFF temperature displays continuously temperature is set to OFF and RH is set to ON RH displays continuously If both are turned ON the display toggles between the two The factory setting defaults to toggle mode if the RH option is present 1 Press the Mode key until the Config indicator lights 2 Press the right arrow until DISP TMP XXX or DISP RH XXX displays in the message center 3 Press up down arrow to toggle the setting ON OFF 4 Press Enter to save the setting 5 Press the Mode key to return to Run mode or right left to go to next previous parameter Thermo Scientific Steri Cycle COz Incubator 3 7 Section 3 Configuration Select a Prima ry Tank On units equipped with the Gas Guard option a primary tank can be selected The primary tank will be either Tank 1 or 2 The
59. e a high mineral content which would produce a build up of scale in the pan High purity or ultra pure water is not recommended as it is an extremely aggressive solvent and will deteriorate the stainless steel Thermo Scientific Filling the Humidity Pan continued Connecting the Thermo Scientific Gas Supply Section 1 Installation and Start Up High purity water has a resistance of above 1M to 18M Ohm Even high purity water can contain bacteria and organic contaminants Water should always be sterilized or treated with a decontaminant safe for use with stainless steel as well as safe for the product prior to being introduced into the humidity pan Caution Distilled or de ionized water used in the humidity pan must be within a water quality resistance range of 50K to 1M Ohm cm to protect and prolong the life of the stainless steel Use of water outside the specified range will decrease the operating life of the unit and may void warranty Check the level and change the water frequently to avoid contamination Do not allow the water level to fluctuate significantly Dry outs will have an adverse effect on the humidity level and calibration of the T C units Warning High concentrations of gas can cause asphyxiation OSHA Standards specify that employee exposure to carbon dioxide in any eight hour shift of a 40 hour work week shall not exceed the eight hour time weighted average of 5000 PPM 0 5 C
60. e sounds signaling that sterilization is complete The Cool Phase begins The display toggles between STERILIZING and COOL PHASE Run Set Cal Config Mode STERILIZING mU Run Set Cal Config T SS ca COOL PHASE eS V Silence Figure 5 17 Cool Phase Thermo Scientific Sterilization Cycle Thermo Scientific continued Section 5 Routine Maintenance 9 Completed Sterilization Cycle with T C CO sensor When the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles between CYC COMPLETE REPL 5 and PRESS ENTER The green LED is lighted but no longer flashes Completed Sterilization Cycle with IR sensor When the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles between CYC COMPLETE POWER OFF and REPLACE IR The green LED is lighted but no longer flashes Clean the IR sensor with either isopropanol or Lysol No Rinse Sanitizer When using isopropanol simply spray the sensor do not saturate and allow to dry With Lysol spray the sensor do not saturate and allow to sit for a couple of minutes Wipe dry with a clean soft cloth Do not saturate the sensor or immerse the sensor in a cleaner Power the unit off open the chamber and remove the IR plate Pull dow
61. e the responsibility of the user 1 Install the large sheet metal side ducts with the tabs facing into the center of the chamber with their slots up There are no right side or left side ducts simply turn one of them to fit the opposite side Tilt the side ducts as they are placed into the chamber so the tops fit into the top air duct then guide them into the vertical position Figure 1 10 shows the side duct as it would be oriented for the right side of the chamber Cm ge Side toward side duct Shelf Channels il Side toward shelf qaaaon annaa 8 3 a S IE i 5 P i e Shelf channel a front slot 5 5 Side un Side Duct 8 Right Side Duct t m with Shelf Channel Installed Figure 1 10 Shelf Channels and Side Ducts Figure 1 11 Channels and Slots Note that there is no difference in the left and right side shelf channels Install the shelf channels by placing the channel s rear slot over the appropriate rear tab on the side duct Pull the shelf channel forward and engage the channel front slot into the side duct s appropriate forward tab Refer to Figure 1 11 Figure 1 10 shows one of the channels installed on the right side duct Steri Cycle CO Incubator 1 7 Section 1 Installation and Start Up Access Port Filter amp 1 Sensor Cover Plate
62. echnical Services at 1 800 438 4851 or 1 740 373 4763 USA and Canada Outside the USA contact your local distributor Repairs NOT Covered Under Warranty Calibration of control parameters Nominal calibrations are performed at the factory typically 1 C for temperature 1 for gases and 5 for humidity Our service personnel can provide precise calibrations as a billable service at your location Calibration after a warranty repair is covered under the warranty Damage resulting from use of improper quality water chemicals or cleaning agents detrimental to equipment materials Service calls for improper installation or operating instructions Corrections to any of the following are billable services 1 electrical service connection 2 tubing connections 3 gas regulators 4 gas tanks 5 unit leveling 6 room ventilation 7 adverse ambient temperature fluctuations 8 any repair external to the unit Damage resulting from accident alteration misuse abuse fire flood acts of God or improper installation Repairs to parts or systems resulting from unauthorized unit modifications Any labor costs other than that specified during the parts and labor warranty period which may include additional warranty on sensors blower motors water jackets etc Thermo Scientific Steri Cycle CO Incubator Thermo Scientific Section 1 Section 2 Table of Contents Installation and Start Up
63. ee Eo co eau erm so Se Power Remote C02 Inlet Air Connector Alarm Contacts 4A Tank Intake 30V max 1A max RJ Il telephone style connectors Used with the optional Gas Guard System IR CO units only Figure 6 5 Unit Back Connections The built in Gas Guard is turned ON when shipped from the factory In addition the Tank Sel for the Gas Guard is specified as Tank 1 when shipped Refer to Section 3 Configuration to de activate the Gas Guard or change the Tank Sel from 1 to 2 If the Gas Guard system is not used the incubator functions normally by supplying CO from the supply tank connected to Inlet 1 With the Gas Guard in operation the incubator uses the gas supplied by the tank connected to Inlet 41 until the pressure drops below 10 psig 0 690 bars At this time the Gas Guard automatically switches to the gas supplied through CO2 Inlet 2 In addition the incubator automatically changes the Tank Sel in Configure Mode from 1 to 2 to indicate that the incubator is now using gas supplied through Inlet 2 If the gas supply to Inlet 1 is replenished the incubator continues using the gas supplied through Inlet 2 unless the operator changes the Tank Sel from 2 to 1 through Configure Mode Refer to Section 3 Configuration Steri Cycle CO Incubator 6 5 Section 6 Factory Options 6 6
64. er installed the air inside the chamber meets the Class 100 air cleanliness standard for par ticulates of 0 5 micron size or larger per cubic foot of air Further infor mation on the Class 100 classification of air quality is available from Thermo Upper and Lower Displays These 7 segment displays vary depending on options present and configuration chosen The upper display can display temp or RH or toggle between them The bottom display shows CO continuously Maintain a minimum three inch clearance behind the incubator for electrical and gas hook ups In addition a three inch ventilation space is needed on each side 2 Locate the unit on a firm level surface capable of supporting the unit s weight of 260 165 118kg 3 Locate the unit away from doors and windows and heating and air conditioning ducts 4 Lift the unit only by the sides of the cabinet base Do not attempt to lift it by the front and back This places stress on the outer door hinges Thermo Scientific Thermo Scientific Stacking the Incubators Section 1 Installation and Start Up Warning Install stacked units against a wall or similar structure A Warning With incubators in a stacked configuration do not leave both exterior doors open at the same time A Warning If the units have been in operation turn them both off and disconnect the power before beginning any service work Two stacking brackets shown at left are included in
65. er on the T C cell must be entered to calibrate the in the unit Note For the technician s convenience a label containing the two numbers on the T C cell is affixed inside the electronics drawer A Press the Mode key until the Config indicator lights Press the right arrow until T CS XXXX displays in the message center Press up down arrow to change the span number to match the sticker Press Enter to save the setting Press the Mode key to return to Run mode or right left to go to next previous parameter Setting Low RH On units with the RH option installed a low RH alarm limit may be Alarm Limit entered The low RH alarm limit is the RH in the cabinet that causes a low RH alarm The setpoint is variable from setpoint 0 to 90 RH The factory setting is 0 RH which disables the alarm 1 2 3 6 Steri Cycle COz Incubator Press the Mode key until the Config indicator lights Press the right arrow until RH LO XX displays in the message center Press up down arrow to change the RH low alarm limit Press Enter to save the RH low alarm limit Press the Mode key to return to Run mode or right left to go to next previous parameter Thermo Scientific Section 3 Configuration Enable RH Alarms to The low RH alarm be programmed to trip the remote alarm contacts Trip Co ntacts A setting of ON allows this a setting of OFF blocks the RH alarm from tripping the contacts The factory setting is ON 1 Press th
66. erefore if a temperature setpoint of 37 C has been entered the humidity pan has been filled and the CO control is to run between 0 1096 with a T C CO sensor the CO setpoint may be entered immediately Otherwise it is important to allow the unit 12 hours to stabilize at the temperature setpoint before entering the setpoint All models of the incubator have a CO2 setpoint range of 0 0 to 20 0 The incubator is shipped from the factory with a setpoint of 0 0 At this setting all control and alarms are turned off To change the CO setpoint 1 Press the Mode key until the Set indicator lights 2 Press the right arrow until CO2 XX X is displayed in the message center 3 Press the up down arrows until the desired CO setpoint is displayed 4 Press Enter to save the setpoint 5 Press the Mode key until the Run indicator lights to go to Run mode or press the right left arrow keys to go to next previous parameter Thermo Scientific Thermo Scientific Section 1 Installation and Start Up set Mode Press MODE to light SET Run Set Cal Config Heat Temp om c3 ca 49 Scroll for Parameters To Set Press MODE to move Operating to CALIBRATE Temperature mode Numbers increase to save setting Scroll for Parameters Numbers decrease Press to return to previous parameter Numbers increase Press Enter to save setting Scroll for Parameters Numbers decreas
67. f the unit has been in service turn it off and disconnect the power cord connector before proceeding with any maintenance A Caution Before using any cleaning method except those recommended by the manufacturer users must check with the manufacturer that the proposed method will not damage the equipment A Use an appropriate disinfectant All articles and surfaces must be thoroughly cleaned rinsed with sterile water and rough dried Warning Alcohol even a 7096 solution is volatile and flammable Use it only in a well ventilated area that is free from open flame If any component is cleaned with alcohol do not expose the component to open flame or other possible hazard Do not spray the T C sensor with flammable solutions The internal temperature of the CO sensor is approximately 150 C when the unit is in operation Allow sufficient time for the sensor to cool before cleaning Do not use strong alkaline or caustic agents Stainless steel is corrosion resistant not corrosion proof Do not use solutions of sodium hypochlorite bleach as they may also cause pitting and rusting A HEPA filter replacement kit see parts list section should be on hand prior to cleaning the incubator interior Steri Cycle COz Incubator 5 1 Section 5 Routine Maintenance Remove the shelves access port filter assembly HEPA filter air sample filter and tubing and the left and right duct sheets Discard the HEPA filters and the access port fil
68. factory settin w Gas Guard Option 1 2 Press the Mode key until the Config indicator lights Press the right arrow until TNK SEL X displays in the message center Press up down arrow to toggle setting between 1 and 2 Press Enter to save the setting Press the Mode key to return to Run mode or right left to go to next previous parameter Disabling the Gas On units equipped with the Gas Guard option the Gas Guard system may Guard System be turned ON or OFF if not in use The factory setting is ON 1 2 3 8 Steri Cycle COz Incubator Press the Mode key until the Config indicator lights Press the right arrow until GAS GRD displays in the message center Press up down arrow to toggle the setting ON OFE Press Enter to save the setting Press the Mode key to return to Run mode or right left to go to next previous parameter Thermo Scientific Set an RS485 Communications Address Thermo Scientific Section 3 Configuration On units with the RS485 option direct communication with the Model 1535 alarm system can be established Each piece of equipment connected to the 1535 must have a unique address An address of 0 24 can be entered for the incubator A setting of 0 is an invalid address that the 1535 ignores The factory setting for the RS485 address is 0 1 2 Press the Mode key until the Config indicator lights Press the right arrow until RS485 XX is displayed in the message ce
69. filter using caution not to touch the filter media 3 Install the filter as shown in Figure 1 13 Refer to Section 5 for HEPA filter maintenance behind top duct Air Sample Filter Figure 1 13 Air Sample Filter Installation Check for level by placing a bubble style level on one of the shelves Turn the hex nut on the leveler counterclockwise to lengthen the leg or clockwise to shorten it Level the unit front to back and left to right See the serial tag on the side of the unit for electrical specifications or refer to the electrical schematics at the end of this manual Caution Serial tag amp rating is based on amperage draw during sterilization cycle Normal operating amperage is much less Ensure that electrical circuit will handle amp draw of sterilization cycle Connect the incubator to a grounded dedicated circuit The power cord connector is the mains disconnect device for the incubator Position the incubator so the unit can be easily disconnected Steri Cycle COz Incubator 1 9 Section 1 Installation and Start Up 1 10 Connecting Unit to Elect Power cont Filling the Humidity Steri Cycle Incubator Pan Plug the provided power cord into the power inlet connector on the back of the cabinet Figure 1 14 then into grounded dedicated electrical circuit Optional A r RS 485 CO2 Inlet eom 2 Tank 75 watts max T
70. he calibration 7 Press the Mode key to return to Run or the right or left arrows to go to the next previous parameter Models 380 and 381 have an infrared IR CO sensor Infrared CO sensors are not effected by chamber atmosphere temperature or humidity However the light detector in the sensor is effected by wide temperature changes Therefore changing temperature setpoints could necessitate a recalibration of the CO Chamber temperature should be allowed to stabilize before checking CO concentrations with an independent instrument especially on start up IR CO Sensor Stabilization Times Start Up Allow the temperature and the of the cabinet to stabilize at least 12 hours before proceeding Presently Operating Allow CO to stabilize at least 2 hours at setpoint before proceeding Steri Cycle CO Incubator 2 3 Section 2 Calibration 2 4 Infrared System continued Relative Humidity Steri Cycle CO Incubator me Measure the CO concentration in the chamber through the gas sample port with a Fyrite or other independent instrument Several readings should be taken to ensure accuracy 2 Press the Mode key until the Cal indicator lights 3 Press the right arrow until IR CAL XX X appears in the message center 4 Press the up down arrow to adjust the display to match the independent instrument reading 5 Press Enter to store the calibration 6 Press the Mode key to return t
71. ity description for humidity pan CCS 8 21730 51 9181 6 15 04 Added alternate 250085 solenoid valve CCS 7 21980 IN 3156 11 07 03 Added min max load and analog output boards max external cable length CCS 6 21273 IN 3147 10 13 03 Factory default for gas guard to ON from OFF CCS 5 21574 IN 3124 8 12 03 Updated temp sensor from 290137 to 290184 glass encapsulated CCS 4 212371 3068 2 24 03 Upgraded inner door components 370 200 9 CCS 20959 IN 3048 10 1 02 Added spacers to power supply mounting CCS 3 20374 IN 2994 11 12 01 Updated 200 6 drawing Kaizen board hardware CCS 20460 Corrected 4 20mA board temp output to 12 5 162 5 C 2 20276 IN 2970 9 24 01 Clarified Step 2 of Section 4 4 CCS 1 19948 IN 2947 9 10 01 Updated 602 inlet labels 200 9 exploded parts drawing CCS 6 28 01 Updates per J Leach art amp list on page 5 4 reference to 4 3 not 4 4 in Alarm section ccs 0 3 1 01 Original manual CCS REV ECR ECN DATE DESCRIPTION By Thermo Scientific Steri Cycle CO Incubator i Preface CAUTION Contains Parts and Assemblies Susceptible to Damage by Electrostatic Discharge ESD Important Read this instruction manual Failure to read understand and follow the instructions in this manual may result in damage to the unit injury to operating personnel and poor equipment performance Caution All internal adjustments and maintenance must be performed by qualified service personnel Material in this manual is for information pu
72. le 0 1V 0 5V or 4 20mA signals Negative display readings will output OV The outputs do not have isolated grounds See Table 6 1 for output specifications of the three boards Table 6 1 Analog Output Board Specifications 190512 4 20 mAOutput 190544 0 1VOutput 190543 0 5VOutput Scaling 4 20 mA Equals Scaling 0 1 V Equals Scaling 0 5V Equals Temperature 12 5 162 5 0 0 150 0 C 0 0 150 0 C RH 0 100 RH 0 100 RH 0 100 RH C02 0 0 100 0 C02 0 100 0 02 0 100 0 C02 6 2 Steri Cycle Incubator Thermo Scientific Analog Output Boards Thermo Scientific continued Section 6 Factory Options For the 0 1V and 0 5V boards the recording device must supply a load gt 1000 ohm For the 4 20mA board the recorder must supply a load of lt 100 ohm To wire in the analog output board use a shielded 22 gauge 3 conductor wire P N 73041 maximum length 50 ft 15 2m This wire is readily available from other vendors including Alpha P N 2403 and Deerborn P N 972203 Caution Accuracy of the output at the board terminal strip to the incubator display is 1 unit There is no calibration from the incubator Calibration to the incubator display must be at the instrument connected to the output board A Front of incubator e coca Analog Board SS E p Microprocessor Board o Blower Motor eus Wire thru port Back of incubator Figure 6 3 Anal
73. libration is used to calibrate various system parameters Configuration allows for custom setup of various options Table 1 1 Selections Under Each Mode Pun SETPOINT CONFIGURATION E a E 1 ww Wami EE CO Hi Alam C0 Z amp 545 po RS485 Address units only Base Unit Displays IR units only Option Displays T C units only Steri Cycle CO Incubator 1 3 Section 1 Installation and Start Up 1 4 Keypad Operation continued Displays Installing the Incubator Steri Cycle CO Incubator Scroll for Parameters Arrows Steps the operator through parameters of SET CAL and CONFIG Modes The right arrow goes to the next parameter the left arrow returns to the previous parameter Up Arrow Increases or toggles the parameter value selected in SET CAL and CONFIG Modes Enter Must press Enter key to save to memory all changed values Down Arrow Decreases or toggles parameter values selected in SET CAL and CONFIG Modes Silence Key Press to mute audible alarm See Section 4 for alarm ringback times Message Center Displays system status Mode at all times Displays CLASS 100 or SYSTEM OK during normal operation or alarm mes sages if the system detects an alarm condition see Section 4 Alarms The display message CLASS 100 is a timing mechanism indicating that under normal operating conditions with the HEPA filt
74. m condition exists Temp Otemp Set point Air Temp Sensor Fault Temperature Controller Failure C02 Sensor Fault Replace HEPA filter reminder set time expired See Section 3 TMP CTRL ERR 0 min C02 SNSR ERR in REPLACE HEPA 0 min DOOR OPEN 15 min 15 min Io es No ES No No No Inner Door is Open C02 is higher than CO2 High Tracking Alarm C02 is lower than CO2 Low Tracking Alarm TEMP is lower TEMP Low Tracking Alarm RH is lower than RH Low Limit Alarm RH option Tank 1 is low switch to Tank 2 Gas Guard only Tank 2 is low switch to Tank 1 Gas Guard only Both tanks are low Gas Guard only All alarm delays and ringback times are 30 seconds C02 IS HIGH 15 min Programmable C02 IS LOW 15 min Programmable TEMP IS LOW 15 min Programmable RH IS LOW in Programmable TANK2 LOW TANK 1 and 2 LOW When multiple alarm conditions occur active messages are displayed in the message center one at a time updating at 5 second intervals Pressing Silence during multiple alarms causes all active alarms to be silenced and to ring back in 15 minutes The TEMP IS LOW alarm is disabled when the Temp set point is 10 C The CO alarms are disabled when the CO set point is 0 096 Thermo Scientific Steri Cycle CO Incubator 4 1 Section 4 Alarms Temp Controller Failure 4 2 TMP CNTR ERR Sensor Fault Alarms Steri Cycle 602 Incubator In addition to other safety features designed int
75. mpanies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermos compliance with this directive the recyclers in your country and information on Thermo products will be available at www thermo com Always use the proper protective equipment clothing gloves goggles etc VY Always dissipate extreme cold or heat and wear protective clothing VY Always follow good hygiene practices v Each individual is responsible for his or her own safety Thermo Scientific Steri Cycle 602 Incubator iii Preface Do You Need Information or Assistance on Thermo Scientific Products If you do please contact us 8 00 a m to 6 00 p m Eastern Time at 1 740 373 4763 Direct 1 800 438 4851 Toll Free U S and Canada 1 740 373 4189 FAX http www thermo com Internet Worldwide Web Home Page service led marietta thermofisher com Service E Mail Address Our Sales Support staff can provide information on pricing and give you quotations We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you Our products are listed on the Internet and we can be contacted through our Internet home page Our Service Support staff can supply technical information about proper setup operation or troubleshooting of your equipment We can fill your needs for spare or replacement parts or provi
76. n on the dummy unscrew the cable from the top of the dummy and press the dummy into the clip on the plate See Figure 5 12 Screw the cable onto the top of the IR sensor The cable is keyed and can only be inserted one way Reinstall the sensor by pushing it as far as it will go into the hole After the unit is powered back on with the IR sensor reinstalled the display toggles between CYC COMPLETE REPL HEPAs and PRESS ENTER Run Set Cal Config mode eve COMPLETE Enter 1 Run Set Cal Config p an nter k Mode REPLACE Sera for Parameters Run Set Cal Config lea e Sac i Figure 5 18 Sensor Reinstalled Steri Cycle 02 Incubator 5 13 Section 5 Routine Maintenance 5 14 Sterilization Cycle continued Steri Cycle Incubator 10 Open the chamber and install a new HEPA filter air sample filter and access port filter assembly Press ENTER 11 When ENTER is pressed the LED goes out the display returns to SYSTEM OK and the HEPA filter replacement timer is reset 12 Fill the humidity pan with 3 liters of sterile distilled water within the recommended purity range See Section 1 13 Allow to stabilize for at least 12 hours at the required temperature and CO level It is recommended that the temperature and CO2 calibrations be verified
77. nce 5 1 Clean Cabinet Exterior 5 2 Clean GlaSS Doors a ee adea vue a CO a 5 3 Clean Humidity epee ep du e RIEN Rd 5 3 Reverse Door Swing 5 3 HEPA Filter Maintenance 5 5 Replace Power 5 5 Replace Air Sample Filter 5 6 Electronics Section Rca Gade cue ea e 5 6 Major 5 7 Sterilization Cycle Stace E I RS ees 5 8 vii Steri Cycle CO Incubator Thermo Scientific Table of Contents Section6 Factory Options 6 1 Remote Alarms eoa MO d th M s a kaos 6 1 RS485 I terfaee A Ud AREIS usta 6 2 Analog Output Boards 6 2 CO Gas Guard 252 6 RUE ia See De 6 4 Connecting Gas Supplies 6 5 De activating Gas Guard 6 5 Gas Guard Operation 6 5 Humidity bI RAM ead teehee ee lees 6 6 Section 7 Specifications 7 1 Section8 Spare Parts 8 1 Exploded Part Drawings
78. nter Press up down arrow to move the RS485 address Press Enter to save the RS485 address Press the Mode key to return to Run mode or right left to go to next previous parameter Steri Cycle COz Incubator 3 9 Section 3 Configuration Configure Mode Press MODE to light CONFIG To Contigure Audible Alarm NEW HEPA Filter REPLACE HEPA Timer Access Code Low Temp Alarm Limit Low Temp Alarm Relay 77 Heat Tomy Se Se eae Br Press MODE to return to RUN mode Press to return ej lo previous parameter Press to return to previous parameler fe mii ee Pam mms less e To reum 10 previous paramelal bor E Plass lt to retum 1a previous parameter tonll ter Press to return 1o previous parameter way e Press to return 10 previous parameter continue on next page 3 10 Steri Cycle CO Incubator Audible alarm ON Prass Enter to save the setting Audible alarm OFF Press Enter to testart the timer and clear the REPLACE HEPA alarm Numbers increase Press Enler lo save the chosen of months Numbers decrease Numbers increase Press Enter lo save the setting Numbers decrease Numbers increase Press Enter To save the setting Numbers decrease Temp relay ON Press Fnter to save the setting lemp relay Thermo Scientific refer to previous page To Configure
79. o Damage by Electrostatic Discharge ESD Steri Cycle COz Incubator 6 1 Section 6 Factory Options RS485 Interface All incubator models can purchased with the RS485 communications option This option allows the incubator to be directly connected to a P N 1900085 Model 1535 alarm system without the use of a communications module A junction box is provided with each RS485 option Refer to Figure 6 2 for wiring details Figure 6 5 shows the location of the RS485 connector on the back of the incubator cabinet To allow the incubator and the 1535 to communicate an address must be allocated on the 1535 Refer to Section 5 8 of the 1535 operating manual The same address number must be assigned to the incubator Refer to Section 3 of this manual A l to additional 1535 to additional 1535 black red modules modules 1 green wire eur 2 1 red wire ZZ 3 yellow wires 00 1 black wire 1535 Junction Box to incubator RJ 11 connector Figure 6 2 RS485 Wiring Analog Output Boards Warning The electronics section contains hazardous voltages Only P N 190512 190543 190544 qualified personnel should access this area The analog output board is an option that allows the incubator to output analog signals representing the air temperature of the cabinet CO2 content and relative humidity depending upon which systems are in the incubator There are three different analog output board options availab
80. o Model 370 Series incubators a thermostat is also provided to monitor the cabinet s temperature In the unlikely event of a temperature control failure the thermostat will turn off all heaters at a cabinet temperature of 160 C 5 This is intended to be a safety feature to protect the incubator and 15 not intended to protect the cell cultures or the equipment inside the chamber should a temperature control failure occur Should such a failure occur contact the Technical Services Department or your local distributor The microprocessor in Model 370 Series incubators continually scans all available sensors to ensure that they are operating properly Should an error be detected the incubator sounds an alarm and displays the appropriate message Contact the Technical Services Department 1 888 213 1790 or your local distributor Thermo Scientific Preventive Maintenance Your equipment has been thoroughly tested and calibrated before shipment Regular preventive maintenance is important to keep your unit functioning properly The operator should perform routine cleaning and maintenance on a regular basis For maximum performance and efficiency it is recommended the unit be checked and calibrated periodically by a qualified service technician The following is a condensed list of preventive maintenance requirements See the specified section of the instruction manual for further details We have qualified service technicians using NIST
81. o Run mode All Model 370 380 Series incubators can be equipped with an optional direct readout relative humidity sensor This is a readout only of the chamber relative humidity level It does not provide any control of the relative humidity in the cabinet Relative Humidity Stabilization Times Start Up Allow 12 hours for the relative humidity and temperature in the chamber to stabilize before proceeding Already Operating Allow at least 2 hours after temperature display reaches setpoint for relative humidity to stabilize before proceeding 1 Place an accurate independent instrument in the center of the chamber Allow at least 30 minutes for RH to stabilize 2 Press the Mode key until the Cal indicator lights 3 Press the right arrow key until RH CAL XX appears in the message center 4 Press the up down arrow to match the display to the independent instrument 5 Press Enter to store the calibration 6 Press the Mode key to return to Run mode Thermo Scientific Section 2 Calibration Relative Humidit If a reliable RH measuring device is not available the display can be 5 p co ntinued calibrated to a typical level 1 Follow the RH stabilization periods outlined above 2 With a full humidity pan and stable temperature the relative humidity in the chamber will be 95 3 Using Step 3 5 of the relative humidity sensor adjustment adjust the display to 95 This calibration method should be accur
82. og Output Board Location To install the analog board 1 Turn off the incubator and unplug it from the wall outlet 2 Remove the screws from the back of the cabinet top to access the electronics area Slide the top back and off 3 Locate the Analog Output board Refer to Figure 6 3 Steri Cycle CO Incubator 6 3 Section 6 Factory Options Analog Output Boards 4 Each system monitored Temp CO2 RH requires two conductors Refer to Figure 6 4 Feed the wires through the auxiliary wire continued through port see above on the back panel This port is a circular fitting adjacent to the remote alarm and RS485 connectors _ f je 1 off 3 5 die d B a ees Ad a S m 0 40 to Zac E roc UUUUUUU UUUUUUUUUUUT e OOOOO Figure 6 4 Board Conductors 5 Strip the ends of each conductor and wire it to the appropriate terminals of connectors J2 and or J3 on the analog board Refer to Figure 6 4 6 When wiring is completed slide on the top of the unit 7 Secure with screws removed earlier and return the unit to service Gas Guard Warning can be lethal in high concentrations Refer to warnings in P N 1900086 Section 1 of this manual A Model
83. oors may be cleaned using the same disinfectant as used on the incubator interior It is imperative that they be rinsed with sterile distilled water to remove the disinfectant residue The doors should then be dried with a soft cloth Some precautions in the cleaning and care of the incubator glass doors Moisture leaches alkaline materials sodium Na from the surface of the glass Evaporation of the moisture concentrates the alkaline and may produce a white staining or clouding of the glass surface Cleaning chemicals with a PH above 9 and heat autoclaving accelerate the corrosion process Therefore it is very important to rinse and dry the glass doors after cleaning Autoclaving the glass doors should be avoided There is no simple method for repairing corroded glass In most cases the glass must be replaced Clean Humidity Pan Clean the humidity pan with soap and water and a general use laboratory disinfectant Rinse with sterile water and spray with 70 alcohol The pan may be autoclaved Reverse Door Swing For side by side operation or changing lab layouts the inner and outer doors are field reversible The procedure is written from the prospective of changing the door swing from a left to a right swing See Figure 5 2 All screw holes are pre drilled for reversing the door The tools required are a Phillips and a flatblade screwdriver This procedure takes about 30 minutes
84. p condition occurs Model 370 380 Series incubators are equipped with a secondary temperature monitoring system to monitor the air temperature inside the cabinet This system is designed as a safety device to turn off all heaters in the event of a temperature control failure Temperature control in the incubator will be 1 of the overtemp setpoint The overtemperature is set by the factory default at 40 C However the overtemp can be set up to 55 C in 0 5 increments If the incubator operating temperature setpoint is set above the overtemp setpoint the overtemp setpoint will automatically update to 1 C above the temperature setpoint It is recommended that the overtemp setpoint be maintained at 1 C over the operating temperature setpoint Thermo Scientific Steri Cycle CO Incubator 1 13 Section 1 Installation and Start Up Setting the Overtemp Setpoint continued Setting the Setpoint 1 14 Steri Cycle CO Incubator To set the Overtemp setpoint Press the Mode key until the Set indicator lights 2 Press the right arrow until Otemp XX X is displayed in the message center 3 Press the up or down arrow key until the desired Overtemp setpoint is displayed 4 Press Enter to save the setting 5 Press the Mode key until the Run indicator lights or press the right or left arrow to go to the next or previous parameter All T C CO cells are calibrated at the factory at 37 C high humidity and 10 CO Th
85. re safety action and indicator Sensor Thermostat independent of temp control system Indicator Message center audible and visual alarms Relative Humidity CO Control Humidity pan natural vaporization Humidity w 95 RH at 37 C Display Optional in 1 increments Alarm Low RH with optional RH monitor Control 0 1 microprocessor PID control SeRSO Raa Aan es T C or IR Beadability cite REUS 0 196 Ranges esse Rete 0 to 20 Inlet pressure 15 psig 1 bar 5 psig 0 3 bar Display Digital LED 0 1 increments Steri Cycle COz Incubator 7 1 Section 7 Specifications 7 2 Steri Cycle 602 Incubator Shelves Dimensions 18 5 x 18 5 47cm x 47cm Construction Stainless steel belt sanded both sides Surface area 2 4 sq ft 0 22 sq m per shelf Max per chamber 38 4 sq ft 3 6 sq m Loading 35 Ibs 16kg slide in and out 50 lbs 23kg stationary Standard 225 Pun nee wht SRP ade a ou eA 4 Maximum d 16 Construction Interior volume 6 5 cu ft 184 liter Interior Type 304 stainless steel shiny finish Exterior yaya 18 gauge cold roll steel Outer door gasket Four sided molded magnetic Santoprene Inner door gasket Bulb silicone Insulation Mineral wool Fittings Ac
86. rmonized International and Nationat Standards EMC EN 61326 1 1997 EN 50081 1 92 EN 50082 1 97 Richard LVD EN 61010 1 1993 Amendments 1 and 2 EN 61010 2 010 CSA C22 2 No 1010 1 UL 61010A 1 Miller Reguiatory Compliance Manager ThermoFisher SCIENTIFIC 13 November 2007 Rev 2 Declaration of Conformity Manufacturer s Name Manufacturer s Address Product Description Product Designations Year of Initia Marking CE Affected Serial Numbers Thermo Fisher Scientific Asheville LLC 401 Millcreek Road Marietta Ohio 45750 USA Steri Cycle Incubator 380 2001 Release 1 Release Level REL shown on Serial Tag This product conforms to the fallowing European Union Directive s EMC LVD 89 336 EEC T3 23 EEC This product conforms to the foliowing Harmonized International and National Standards EMC EN 61326 1 1997 EN 50081 1 92 EN 50082 1 97 LVD EN 61010 1 1993 Amendments 1 and 2 EN 61010 2 010 CSA 22 2 No 1010 1 UL 81010A 1 7222 0 aa Richard L Miller CQE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 13 November 2007 Thermo Scientific Rev 2 Declaration of Manufacturer s Name Thermo Fisher Scientific Asheville LLC Manufacturer s Address 401 Millcreek Road Marietta Ohio 45750 U S A Product Description Steri Cycle Incubator Product Designations 371 Year of Initial Marking 2001 Affected
87. rposes only The contents and the product it describes are subject to change without notice Thermo Scientific makes no representations or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental arising out of or related to the use of this manual Warning If the incubator is not used in the manner specified in this operating manual the protection provided by the equipment design may be impaired 2003 Thermo Scientific All rights reserved Steri Cycle Incubator Thermo Scientific Preface Important operating and or maintenance instructions Read the accompanying text carefully Potential electrical hazards Only qualified persons should perform procedures associated with this symbol Equipment being maintained or serviced must be turned off and locked off to prevent possible injury Hot surface s present which may cause burns to unprotected skin or to materials which may be damaged by elevated temperatures Marking of electrical and electronic equipment which applies to electrical and electronic equipment falling under the Directive 2002 96 EC WEEE and the equipment that has been put on the market after 13 August 2005 This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 It is marked with the WEEE symbol Thermo Scientific has contracted with one or more recycling disposal co
88. stall the cover Remove the door handle by first carefully prying off the three nylon screw covers Cord cover Figure 5 6 Cord Cover Location Then remove the three screws Set aside Remove the hinges from the outer door Carefully pry off the nylon screw cover from between the hinges Remove the screw Install the hinges on the opposite side of the door Install the screw and screw cover between the hinges Thermo Scientific Section 5 Routine Maintenance Reverse Door Swing 11 Install the door handle opposite the hinges Install screw covers continued 12 To secure the outer door to the unit the Phillips screws on the side of the unit must be removed and installed on the opposite side 13 Secure the outer door hinges to the unit 14 Locate the small cover plate on the side of the unit Refer to Figure 5 7 Remove the two screws and move this plate to the opposite side of the unit Secure the plate o Cover plate Figure 5 7 Cover Plate Location 15 Install the door cord to the connector Secure the plate with the two screws See Figure 5 3 16 Return the unit to service HEPA Filter Replace the HEPA filter when the REPLACE HEPA reminder is Maintenance displayed The REPLACE HEPA reminder can be set to alarm after a specified time from 1 to 12 months The reminder default is the factory recommended setting of 6 months For details see Section 3 Replace Power Fuses a
89. t all reference instruments be properly calibrated Tempe rature Before calibration allow the cabinet temperature to stabilize Place the calibrated instrument in the center of the chamber The instrument should be in the airflow not against the shelf Temperature Stabilization Periods Startup Allow 12 hours for the temperature in the cabinet to stabilize before proceeding Already Operating Allow at least 2 hours after the display reaches setpoint for temperature to stabilize before proceeding Thermo Scientific Steri Cycle COz Incubator 2 1 Section 2 Calibration Temperature 1 Press the Mode key until Cal indicator lights continued 2 Press the right arrow until TEMPCAL XX X appears in the message center 3 Press up down arrow to match display to calibrated instrument 4 Press Enter to store calibration 5 Press the Mode key to return to Run or the right left arrow to go to next previous parameter Thermal Conductivity Models 370 and 371 have a thermal conductivity T C CO2 sensor CO System Thermal conductivity of the incubator atmosphere is not only effected by the quantity of CO present but also by the air temperature and water vapor present in the incubator atmosphere In monitoring the effects of air temperature and absolute humidity must be held constant so any change in thermal conductivity is caused by a change in CO2 concentration Changing temperature or changing from elevated humidit
90. tatus Mode Select Indicators Run Run Menu Set Set Points Menu Cal Calibrate Menu Config Configuration Menu Up and Down Arrows Increases or decreases number values toggles between choices Enter Stores the value into computer memory Heat Indicator Lights when power is applied to heaters Temp Display Programmable to display temperature continuously RH continuously with RH option or toggle between temperature and humidity with RH option See Configuration Section Scroll for Parameters Arrows Moves the operator through selected mode choices Inject Indicator Lights during injection into chamber 2 Display Displays CO percentage continuously Alarm Heat Indicator SIR Up Down Indicator Temp t Indicators Silence Model Select Arrows Display Switch AN Enter Run Set Cal Config Heat Temp mm 36 9 Scroll for Parameters STERILIZING Inject 49 96 CO 96 C0 Scroll for CO Inject Display Parameters Indicator Figure 1 2 Control Panel 1 2 Steri Cycle COz Incubator Thermo Scientific Section 1 Installation and Start Up Keyp ad Ope ration The Model 370 Series incubator has four basic modes which allow Thermo Scientific incubator setup Run Setpoints Calibration and System Configuration e Run is the default mode in which the incubator will normally be during operation Set is used to enter system setpoints for incubator operation Ca
91. ter assembly See Figure 5 1 Filter Access Air Sample Port Filter Filter and stopper and tubing assembly Humidity Pan Figure 5 1 Component Locations 2 Wash the air sample filter tubing shelves and ducts with disinfectant then rinse with sterile water Optionally the shelves and ducts may be autoclaved Wash the inner door gasket with disinfectant This gasket may be removed to be cleaned or replaced Wash the cabinet interior with disinfectant starting at the top and working down Refer to the disinfectant directions for length of time needed before rinsing Wash the inner door both inside and out The cabinet and door must be rinsed with sterile water until the disinfectant has been removed After the cabinet has been rinsed spray with 70 alcohol Install the left and right ducts inner door gasket access port filter assembly and air sample filter and tubing spraying each with 70 alcohol Install a new HEPA filter Install the shelves and spray with 70 alcohol Clean Cabinet Clean the incubator exterior with a damp sponge soft well wrung cloth P spong g Exterior and mild detergent dissolved in water Dry with a soft cloth 5 2 Steri Cycle COz Incubator Thermo Scientific Section 5 Routine Maintenance Clean Glass Doors The chamber glass door and the optional independent inner glass d
92. tions Front of incubator DC power supply AT Power switch Analog Board Pe 4 20mA 0 1V 0 5V Component fan sensor IR or TC thermostat Sample port 2 2 thermostat into chamber board Main power transformer gas solenoid Component fan Filtered line cord 7 1414 connector RS 485 and FF Back of incubator remote alarm contacts 00 manifold and Accessory connectors and Supply tank connectors outlet 75W max wire through put 085 guard factory installed option Figure 5 9 Electronics Drawer Thermo Scientific Steri Cycle COz Incubator 5 7 Section 5 Routine Maintenance Sterilization Cycle Information You Need to Know Before Starting a Cycle The Sterilization Cycle requires approximately 12 hours heat up 2 4 hours sterilize 2 hours and cool down 6 8 hours Additional time is needed to verify the calibration of temperature and CO after the cycle is complete During the Sterilization Cycle the incubator updates the temperature to the analog output board and the 1535 however CO2 will be fixed at setpoint and RH will be fixed at RH Low Limit plus 1 A HEPA filter replacement kit see the spare parts list should be on hand prior to initiating the Sterilization Cycle Information About the Cycle Pre cleaning may be required In order to avoid odors stains on the interior baked on material etc wipe off
93. to power the humidity pan filled and the unit connected to gas supplies system setpoints can be entered The following setpoints can be entered in Set mode Temperature Overtemperature and CO To enter Set mode press the Mode key until the Set indicator lights Press the right and or left arrow keys until the proper parameter appears in the message center See Chart 1 1 for more detail All Model 370 380 Series incubators have an operating temperature range of 10 C to 50 C depending on ambient temperature The incubator is shipped from the factory with a temperature setpoint of 10 C At this setting all heaters are turned off Thermo Scientific Section 1 Installation and Start Up Setting the Operating To change the operating temperature setpoint Temperature continued Press the Mode key until the Set indicator lights 2 Press the right arrow until Temp XX X is displayed in the message center 3 Press the up down arrow key until the desired temperature setpoint is displayed 4 Press Enter to save the setpoint 5 Press the Mode key until the Run indicator lights for Run mode or press the right left arrow keys to go to next previous parameter Setting the Overtemp Caution The independent overtemp system is designed as safety to Setpoint Protect the incubator only It is not intended to protect or limit the maximum temperature of the cell cultures or customer s equipment inside the incubator if an overtem
94. traceable instruments available in many areas For more information on Preventive Maintenance or Extended Warranties please contact us at the number listed below Cleaning and calibration adjustment intervals are dependent upon use environmental conditions and accuracy required Tips for all incubators Do NOT use bleach or any disinfectant that has high chloros _ e Avoid spraying cleaner on the CO sensor Use sterile distilled or demineralized water e Do not use powdered gloves for tissue cultures 401 Millcreek Road Box 649 Marietta Ohio 45750 USA 740 373 4763 USA and Canada 888 213 1790 Telefax 740 373 4189 http www services controlenv thermo com Preventive Maintenance for Model 370 380 Series Incubators Referto Manual Action Daily Weekly Monthly 3to6 Yearly Section Months Check 02 tank levels v Inspect door latch hinges and door gasket seal 1 Check water level in the humidity 74 from top Y 2 Verify and document CO2 humidity and temperature calibration as v applicable See Calibration Disinfect the interior of the incubator See Routine Maintenance Y 1 Replace HEPA access port filter air sample filter and CO2 filters if v applicable or as needed 5 Perform sterilization cycle as needed Thermo Scientific Steri Cycle CO Incubator 4 3 Thermo Scientific Section5 Routine Maintenance Warning I
95. y to room ambient humidity levels will necessitate a re calibration of the control T C CO Sensor Stabilization Periods Some T C CO sensors go through an aging period especially on new installations Calibration should be checked on a weekly basis and adjusted as necessary When stabilization occurs checks may become less frequent Start Up The CO sensor has been calibrated at the factory for 37 C and elevated humidity Allow the temperature humidity and levels in the chamber to stabilize at least 12 hours before checking the concentration with an independent instrument Presently Operating Make sure the chamber doors are closed Allow at least 2 hours after the temperature and displays reach their setpoints for chamber atmosphere stabilization 2 2 Steri Cycle 602 Incubator Thermo Scientific Thermal Conductivity System continued Infrared System Thermo Scientific Section 2 Calibration me Make sure the stabilization periods outlined above are followed 2 Sample the chamber atmosphere through the sample port with an independent instrument Sample the atmosphere at least 3 times to ensure accuracy of the instrument 3 Press the Mode key until the Cal indicator lights 4 Press the right arrow until CO2 CAL XX X is displayed in the message center 5 Press the up down arrows to change the display to match the independent instrument 6 Press Enter to store t
96. ycle 602 Incubator vi Table of Contents Section 3 Configuration 3 1 Turn Audible Alarm ON OFF 3 1 New HEPA Filiere uku ED 3 1 Set REPLACE HEPA filter reminder 3 2 Set Access Code yo anda end dish BS wae bleu bud bow das 3 2 Set Low Temp Alarm Limit tracking alarm 3 3 Enable Low Temp Alarm to Trip Contacts 3 3 Set Low CO Alarm Limit tracking alarm 3 4 Set High CO Alarm Limit tracking alarm 3 4 Enable CO Alarms to Trip Contacts 3 5 Set New Zero Number for T C CO2 Sensors 3 5 Set New Span Number for T C CO Sensors 3 6 Setting a Low RH Alarm Limit 3 6 Enable RH Alarms to Trip Contacts 3 7 Enabling Temp RH to be Displayed 3 7 Select a Primary Tank w Gas Guard Option 3 8 Disabling the Gas Guard System 3 8 Set an RS485 Communications Address 3 9 Section 4 Alarms Tae su IL T LL 4 1 Temp Controller Failure TMP CNTRERR 4 2 Sensor Fault Alarms 4 2 Preventive Maintenance 4 3 Section5 Routine Maintena

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