Safety Stand User`s Manual
Contents
1. cables to the output on the rear or the front panel of the electroporator depending on the BTX electroporator being used 2 Using aseptic technique pipette the correct volume of cell suspension and reagents into the cuvette or flatpack chamber If using a cuvette replace the cap 3 Open the safety stand shield and place the cuvette or flatpack chamber between the metal contacts Adjust the distance between the contacts using the black roller Note the adjustment will remain the same after the first time do not readjust the roller with every cuvette however check contact periodically to ensure good current flow Close the shield 4 Switch on the BTX electroporator Check that all instrument settings and connections are correct Deliver the electroporation pulse by pressing the appropriate START or PULSE button depending on the BTX electroporator being used 5 After the pulse is delivered remove the cuvette or flatpack chamber from the safety stand Use a pipette to remove the pulsed cells Treat the pulsed cells according to the protocol 6 Discard the cuvette pipette and or flatpack chamber in a biohazard container Refer to your country s waste management organization for proper disposal practices 7 Unplug safety stand from electroporator Clean plastic lid and metal contacts with ethanol and deionized water as needed APPENDIX A SPECIFICATIONS Safety Stand Electrical amp Technical Specifications Standard2. Capabilities Voltage Range Frequency Pulse Length Time Constants Range Pulse Number Range Operating Temperature Intended Use Relative Humidity Maximum Altitude Pollution Degree Insulation Category Physical Characteristics Overall Dimensions LxWxD Weight toll free 800 272 2775 s 0 to 3000 VDC 0 to 250 VAC 1 MHz 10 usec to 10 sec 1 to 99 depending on voltage 5 to 40 C Indoor use only 20 to 80 2 000 m 6 562 ft II CATI 12 7 x 10 2 x 17 8 cm 5x4x7in 1 kg 2 3 Ibs local 508 893 Compatibility Generators ECM 399 630 830 and 2001 Monitoring The Enhancer 3000 Monitoring System Note Depending on buffer composition volume and generator capability Note For compatibility with older models call BTX technical support APPENDIX B REPLACEMENT PARTS Order No Model Description 45 0207 630B Safety Stand 45 0213 BT630 Acrylic Safety Stand Cover 45 0215 Metal Replacement Kit 45 0208 660 Cuvette Rack 45 0140 613 Bulk Pack of Cuvettes 1 mm gap 24 pkgs of 100 45 0141 623 Bulk Pack of Cuvettes 2 mm gap 24 pkgs of 100 45 0142 643 Bulk Pack of Cuvettes 4 mm gap 24 pkgs of 100 45 0125 620 Disposable Cuvettes 2 mm gap pkg 50 45 0126 640 Disposable Cuvettes 4 mm gap pkg 50 45 0109 485 Flatpack Chambers 0 56 mm gap pkg 50 45 0110 486 Flatpack Chambers 1 53 mm gap pkg 50 45 0059 VIP3000SC The Enhancer 3000 Monitoring System Meets requir
3. Safety Stand HARVARD APPARATUS The Electroporation Experts INTRODUCTION The BTX Safety Stand is a safety device for use with the disposable cuvettes and the flatpack chambers Its unique design ensures that no current can be delivered unless the lid is closed therefore protecting the user from high voltages Up to two cuvettes can be electroporated at once thus decreasing experimentation times It is compatible with the BTX electroporators listed below IMPORTANT Read all Instructions Warnings and Precautions prior to use FOR RESEARCH PURPOSES ONLY tollfreee 800 272 2775 ioca 508 893 Compatible BTX electroporators ECM 830 ECM 630 ECM 399 ECM 2001 Note For compatibility with older models call BTX technical support Order No Model Description 45 0207 630B Safety Stand 8999 www btxonline com Safety Stand GENERAL INFORMATION Warranty BTX Harvard Apparatus warranties this BTX Safety Stand for a period of 90 days from date of purchase At its option BTX Harvard Apparatus will repair or replace the item if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchase IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incide
4. TX is the ultimate resource for technical information on the use of high voltage bacterial transformation and general electroporation of molecules and drugs into cells We constantly track and monitor scientific publications in this area Our Technical Service group extracts and enters pertinent information such as results and parameters from these papers into a Protocol database This database is available via the BTX website Please visit www btxonline com For technical assistance additional information or an inquiry request for repair service contact BTX Harvard Apparatus Technical Support Customer Service Group at BTX A Division of Harvard Apparatus 84 October Hill Road Holliston MA 01746 1388 U S A Toll Free 1 800 272 2775 U S only Phone 1 508 893 8999 Fax 1 508 429 5732 E mail techsupport btx harvardapparatus com Internet www btxonline com click on customer service If outside the United States and Canada call 1 508 893 8999 or contact your nearest BTX Distributor A complete list of distributors is on our website toll free 800 272 2775 s local 508 893 GENERAL SAFETY INFORMATION Review the following safety precautions to avoid injury and prevent damage to this product or any products connected to it To avoid potential hazard use this product only as specified Only qualified BTX personnel should perform service procedures To Prevent Hazard or Injury ARCING CAN OCCUR AT HIGH VOLTAGES An unfavorab
5. ements of Directive 89 336 EEC for Electromagnetic Compatibility EC and Low Voltage Directive 72 23 EEC for Product Safety APPENDIX C TROUBLESHOOTING Please contact BTX Technical Service at any of the numbers listed below in the event of any failure BTX A Division of Harvard Apparatus 84 October Hill Road Holliston MA 01746 1388 U S A Toll Free 1 800 272 2775 US only Phone 1 508 893 8999 Fax 1 508 429 5732 E mail techsupport btx harvardapparatus com Internet www btxonline com click on customer service CAUTION FOR RESEARCH USE ONLY NOT FOR CLINICAL USE ON PATIENTS Safety Stand Model 630B 8999 www btxonline com WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive a
6. le combination of parameters such as high voltage settings and a small sample volume with a highly conductive medium might lead to flashover between the electrodes ARC and or explosive evaporation of the medium Reduce voltage or pulse length to avoid repeating this condition DO NOT OPERATE WITH SUSPECTED FAILURES If you suspect there is damage to the product have it inspected by qualified BTX service personnel DO NOT CONTACT ELECTRODES To avoid fire or shock hazard observe all ratings and markings on the product or in this manual before using the device AVOID EXPOSURE TO CONTACT Do not insert fingers or try to remove electrode or sample during pulsing sequence WEAR PROPER EYE PROTECTION DURING ELECTROPORATION DO NOT OPERATE IN AN EXPLOSIVE ENVIRONMENT DO NOT OPERATE IN WET DAMP CONDITIONS Safety Terms and Symbols Terms that appear in this manual WARNING Warning statements identify conditions or practices that could result in injury or loss of life CAUTION Caution statements identify A conditions or practices that could result in Symbols that may appear on the products damage to these products or other property A A Danger Attention Protective Functional High Refer to Earth Ground Voltage Manual Terminal Terminal 8999 www btxonline com Safety Stand OPERATION GETTING STARTED A WARNING HIGH VOLTAGE Make sure the BTX electroporator is switched off before continuing 1 Attach the safety stand
7. ntal or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the 90 day warranty period promptly contact BTX Harvard Apparatus 84 October Hill Road Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 Outside the U S call 1 508 893 8999 Goods will not be accepted for return unless an RMA Return Materials Authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs or replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Note BTX electrodes are not recommended for use with power supplies or cables from other manufacturers Such use is completlely at the customer s own risk as it may result in damage create unsafe conditions and will immediately void the 90 day warranty IMPORTANT Read all Instructions Warnings and Precautions prior to use Technical amp Customer Service B
8. vailable for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products Do Not Dispose Product with Municipal Waste and Category 9 monitoring and control instruments Most of our e Special Collection Disposal Required products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any changes as they apply HARVARD APPARATUS The Electroporation Experts 84 October Hill Road e e Holliston MA 01746 toll free 800 272 2775 local 508 893 8999 fax 508 429 5732 emai Te chsupport btx harvardapparatus com gt weowww btxonline com Publication 5502 002 REV CS
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