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Agilent 1290 Infinity II Flexible Pump User Manual

1. Agilent 1290 Infinity I Flexible Pump User Manual 91 7 Maintenance Replace the Inlet Valve Replace the Inlet Valve When If Inlet valve is defective Tools required p n Description Wrench 14 mm G4220 20012 Torque wrench 2 25 Nm Parts required p n Description G4204 60022 Inlet Valve 1290 Infinity Quaternary Pump Preparations Switch off pump at the main power switch Open the doors Use an optional solvent shutoff valve or lift up solvent filters inside solvent bottles for avoiding leakages Remove the inlet weaver see Replace the Inlet Weaver on page 90 1 With a 14 mm wrench unscrew the inlet valve and 2 Install the new inlet valve and tighten it using a torque remove it wrench with a 14 mm bit set to 10 Nm Next Steps 3 Insert the inlet weaver see Replace the Inlet Weaver on page 90 4 Purge the system to remove air 92 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Replace the Outlet Valve Replace the Outlet Valve When If Outlet valve is defective Tools required p n G4220 20012 G4220 20041 Parts required p n G4220 60028 G4220 20020 Description Wrench 14mm Torque wrench 2 25 Nm Bit Torx 10x25 mm Description Outlet valve primary pump head Internal gold seal for Outlet Valve Preparations Switch off pump at the main power switch Open the doors Use an optional solvent shutoff valve or lift up solve
2. gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path Agilent 1290 Infinity II Flexible Pump User Manual 85 7 Maintenance Install Fittings and Capillaries Solvent can spray under high pressure gt Observe appropriate safety procedures for example goggles safety gloves and protective clothing when opening flow path CAUTION Deformation of fittings and seals Liquid drops under high pressure up to 1200 bar act like solid parts Tightening connections under high pressure can deform or destroy fittings and seals gt Never tighten flow connections under pressure 86 The lifetime of a fitting depends on how firmly it has been tightened firm tightening reduces the lifetime If fitting has been overtightened replace it 1 Install fittings and capillaries 2 Tighten fittings and capillaries Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Remove and Install Doors Remove and Install Doors Parts required p n Description 5067 5745 Door Assy Infinity 180 Left 5067 5746 Door Assembly Infinity 180 Right The figures shown in this procedure exemplarily show the Infinity II Multisampler module The principle of how to remove and or install doors works in the same way for all Infinity II modules 2 For the Installation of the front door Insert the hinges into their guides and move the door
3. writing the pump encoder tag failed 64 extra column volume 40 F fan failed 59 firmware description 1 4 main system 174 resident system 1 74 update tool 175 updates 175 140 upgrade downgrade 140 fittings 86 flow rate limit exceeded 62 frequency range 27 G gasket replace 120 general error messages 54 heat exchanger replace 117 high pressure filter frit replace 136 high pressure filter replace 136 humidity 27 initialization mode selection 193 inlet valve Agilent 1290 Infinity I Flexible Pump User Manual Index release if stuck 102 replace 92 stuck 102 inlet weaver replace 90 inline filter replace parts 138 installation bench space 26 site requirements 23 install doors 87 jet weaver 97 pump head assembly 131 instrument layout 188 insufficient power of drive encoder LED 70 interfaces 48 Infinity Il 179 internet 229 J jet weaver install 97 remove 95 L LAN automatic configuration with Bootp 201 Bootp amp Store 194 Bootp 193 cable 1 0 configuration switch 192 configuration 189 first steps 190 initialization mode selection 193 link configuration selection 200 manual configuration with telnet 212 231 Index manual configuration 211 PC and User Interface Software Setup 216 TCP IP parameter configuration 191 using default 195 using stored 195 leak sensor open 57 leak sensor short 56 leak 59 line frequency 27 line voltage 27 link co
4. 9 G7104 60001 10 64204 68000 11 64204 60004 5067 5716 Parts and Materials 8 Description In Line Filter Assembly for 1290 Infinity Quaternary Pump Frit 0 3 pm for inline filter 5 pk Clamp for In Line Filter Multi Purpose Valve Head Inlet Valve 1290 Infinity Quaternary Pump 1290 Infinity Quaternary Pump Inlet Weaver Assembly Multi channel gradient valve MCGV Blank plug for MCGV Pump head assembly G7104A Outlet valve primary pump head Peristaltic pump with Pharmed tubing Pressure sensor 1300 bar Jet Weaver 380 uL for 1290 Infinity Quaternary Pump OPTIONAL Outlet filter 1290 Infinity Quaternary Pump Frit for 1290 pump outlet filter 2 pk Agilent 1290 Infinity II Flexible Pump User Manual 145 8 Parts and Materials Flow Connections Flow Connections 4 3 2 5 1 6 7 8 Figure 12 Flow connections of the pump 146 Agilent 1290 Infinity II Flexible Pump User Manual Item p n 1 G4220 60035 2 G7120 60007 5067 5760 3 5065 9978 4 5065 4445 5 5067 4656 6 5067 4748 7 G4220 67000 8 5500 1217 5067 6129 5067 5416 Parts and Materials Description Tubing kit 140 mm 2 pk Degasser to MCGV Bottle Head Assembly Solvent Cabinet Kit not shown Tubing 1 mm i d 3 mm o d silicone 5 m Peristaltic pump with Pharmed tubing Capillary ST 0 25 mm x 80 mm Pressure Sensor to Outlet Filter to Pump Head and to Multi Purpose Valve Capillary ST 0 17 mm x 90 mm Multi Purpose Val
5. Probable cause 1 The waste container is full 2 Inappropriate setting for waste counter Flow rate limit exceeded Error ID 29164 Suggested actions Empty waste container e Reset waste counter e Adjust waste counter limit The flow rate of the quaternary pump has exceeded the limit while the pump runs in pressure controlled mode e g during a pressure test e Parameter None Probable cause 1 Leak 2 Bottle empty 3 Shutoff valve closed if applicable 4 Drift of pressure sensor unlikely for short tests taking some minutes 62 Suggested actions Check for leaks in the pump and flow path Fill solvent bottle Open shutoff valve Replace pressure sensor Agilent 1290 Infinity II Flexible Pump User Manual Error Information 6 Quaternary pump shutdown during analysis Error ID 29199 The quaternary pump has been shut down by the control software or control module during an analysis e Parameter 0 for off 1 for standby Probable cause Suggested actions 1 Pump has been shut down Restart pump Reading the pump encoder tag failed Error ID 29201 Reading the pump encoder tag has failed e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Defect connection between encoder and Please contact your Agilent service main board representative 2 Missing or defect tag Defect connection Please contact your Agilent service between tag and encoder representative
6. ote Agilent Technologies Notices Agilent Technologies Inc 2014 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 67104 90000 Rev B Edition 12 2014 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document
7. TCP IP Statu Controllers Figure 42 214 connected to PC with controller software e g Agilent 134 40 27 95 ChemStation here not connected 255 255 248 0 134 40 24 11 134 40 27 99 255 255 248 0 134 490 24 1 no connections Telnet Change IP settings Agilent 1290 Infinity II Flexible Pump User Manual LAN Configuration 11 7 When you have finished typing the configuration parameters type exit and press Enter to exit with storing parameters cx WINDOWS system32 cmd exe Agilent Technologies G4296A DEGHBHQH06 gt exit Connection to host lost CoN Figure 43 Closing the Telnet Session If the Initialization Mode Switch is changed now to Using Stored mode the instrument will take the stored settings when the module is re booted In the example above it would be 134 40 27 99 Agilent 1290 Infinity II Flexible Pump User Manual 215 11 LAN Configuration PC and User Interface Software Setup Setup PC Setup for Local Configuration This procedure describes the change of the TCP IP settings on your PC to match the module s default parameters in a local configuration see also Initialization Mode Selection on page 193 FIXED IP address Local Area Connection Properties 2 x Internet Protocol General General Connect using You can get IP setings assigned automatically if your network supports this capability Otiierwise you need to ask your network administrator
8. instrument configuration information and diagnostic tests By the push of a button a detailed diagnostic report can be generated Upon request the user can send this report to Agilent for a significantly improved troubleshooting and repair process The Agilent Lab Advisor software is available in two versions e Lab Advisor Basic Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Infinity II Series pump The Lab Advisor Advanced features can be unlocked by purchasing a license key and include real time monitoring of instrument actuals all various instrument signals and state machines In addition all diagnostic test results calibration results and acquired signal data can be uploaded to a shared network folder The Review Client included in Lab Advisor Advanced allows to load and examine the uploaded data no matter on which instrument it was generated This makes Data Sharing an ideal tool for internal support groups and users who want to track the instrument history of their analytical systems The optional Agilent Maintenance Wizard Add on provides an easy to use step by step multimedia guide for performing preventive maintenance on Agilent 1200 Infinity and Infinity II Series instruments The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help fil
9. 2 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Agilent 1290 Infinity II Flexible Pump User Manual 69 6 6 Error Information Drive position limit Error ID 29234 Internal error Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Internal error Please contact your Agilent service representative Insufficient power of drive encoder LED Error ID 29235 Insufficient power of drive encoder LED Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Drive encoder error Error ID 29237 29238 29239 29215 An error has occurred for the pump drive encoder Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative 70 Agilent 1290 Infinity II Flexible Pump User Manual Error Information Writing the multi purpose valve tag failed Error ID 29241 Writing the multi purpose valve tag failed e Parameter None Probable cause Suggested actions 1 Multi purpose valve head tag defect Replace multi purpose valve head 2 Multi purpose valve tag head reader is Please contact your Agilent service defect representative Unknown multi purpose valve type Error ID 29242 The type infor
10. Agilent 1290 Infinity II Flexible Pump User Manual 63 6 Error Information Writing the pump encoder tag failed Error ID 29200 Writing the pump encoder tag has failed e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Defect connection between encoder and Please contact your Agilent service main board representative 2 Defect tag Defect connection between tag Please contact your Agilent service and encoder representative Pump drive blocked or encoder failed Error ID 29214 Pump drive blocked or encoder failed e Parameter None Probable cause Suggested actions 1 Blockage of the pump drive Drive encoder Please contact your Agilent service failed representative 64 Agilent 1290 Infinity II Flexible Pump User Manual Error Information 6 Drive current too low Error ID 29205 The current consumption of the pump drive is too low e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Drive motor defect Please contact your Agilent service representative 2 Wrong missing connection of pump drive to Please contact your Agilent service main board representative Drive current too high Error ID 29236 The current consumption of the pump drive is too high e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Blockage of system before pressure sensor Check for blockage of e g outlet valve filter frit Multi Purpose Valve h
11. BootP LogFile It contains a MAC address entry for each device that requests configuration information from BootP 3 Click OK to save the values or Cancel to discard them The editing ends 4 After each modification of the BootP settings i e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service on page 209 or Restarting the Agilent BootP Service on page 210 5 Uncheck the Do you want to log BootP requests box after configuring instruments otherwise the log file will quickly fill up disk space Agilent 1290 Infinity II Flexible Pump User Manual 205 11 LAN Configuration 206 Determining the MAC address directly from the LAN Interface card label 1 Turn off the instrument 2 Read the MAC address from the label and record it The MAC address is printed on a label on the rear of the module 3 Read the MAC address from the label and record it The MAC address is printed on a label on the rear of the module See Figure 23 on page 190 and Figure 24 on page 190 4 Turn on the instrument Assigning IP Addresses Using the Agilent BootP Service The Agilent BootP Service assigns the Hardware MAC address of the instrument to an IP address Determining the MAC address of the instrument using BootP Service 1 Power cycle the Instrument 2 After the instrument completes self test open the log file of the BootP Service using Notep
12. It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 8 Boot Resident Settings On board LAN Mode Select sw1 sw2 SW3 sSw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 1 0 0 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table9 Forced Cold Start Settings On board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 sws TEST BOOT 1 1 0 0 0 0 0 1 186 Agilent 1290 Infinity II Flexible Pump User Manual Hardware Information 10 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides
13. Solvent Type Catalogs In Solvent Type Catalogs click Import Navigate to the location of the solvent calibration table and click Open ao ol A Q The new solvent will now appear in the Solvent Type Catalogs Agilent 1290 Infinity II Flexible Pump User Manual Agilent 1290 Infinity II Flexible Pump User Manual KP e e 20 Gee 5 7 e Troubleshooting and Diagnostics e amp 8 7 5 User Interfaces 48 Agilent Lab Advisor Software 49 Overview about the troubleshooting and diagnostic features te Agilent Technologies 47 5 Troubleshooting and Diagnostics User Interfaces Depending on the user interface the available tests and the screens reports may vary e Preferred tool should be Agilent Lab Advisor Software see Agilent Lab Advisor Software on page 49 The Agilent OpenLab ChemStation C 01 03 and above do not include any maintenance test functions e Screenshots used within these procedures are based on the Agilent Lab Advisor Software 48 Agilent 1290 Infinity II Flexible Pump User Manual Troubleshooting and Diagnostics 5 Agilent Lab Advisor Software The Agilent Lab Advisor Software is a standalone product that can be used with or without chromatographic data system Agilent Lab Advisor helps to manage the lab for high quality chromatographic results by providing a detailed system overview of all connected analytical instruments with instrument status Early Maintenance Feedback counters EMF
14. a higher plate number The pressure rises with the inverse square of the particle size and proportionally with the length of the column This is the reason that the 1290 Infinity LC system was designed to go to 1200 bar so that it can run sub two micron particles and column length can be increased to 100 mm or 150 mm There are even examples of 100 mm and 150 mm columns linked to give 250 mm length Resolution increases with the square root of N so doubling the length of the column will increase resolution by a factor of 1 4 What is achievable depends on the viscosity of the mobile phase as this relates directly to the pressure Methanol mixtures will generate more back pressure than acetonitrile mixtures Acetonitrile is often preferred because peak shapes are better and narrower in addition to the lower viscosity but methanol generally yields better selectivity certainly for small molecules less than about 500 Da The viscosity can be reduced by increasing the temperature but it should be remembered that this can change the selectivity of the separation Experiment will show if this leads to increase or decrease in selectivity As flow and pressure are increased it should be remembered that frictional heating inside the column will increase and that can lead to slightly increased dispersion and possibly a small selectivity change both of which could be seen as a reduction in resolution The latter case might be offset by reducing the temperature of
15. analytical efficiency The established multipurpose valve enhances laboratory efficiency by adding useful functionalities for example mixer in out switch filter backflush or automatic purge and BlendAssist software simplifies your workflow with accurate buffer additive blending Status indicator Pressure sensor Pump head Inlet weaver Power switch Leak drain Figure 1 Overview of the Flexible Pump 10 Agilent 1290 Infinity II Flexible Pump User Manual Introduction 1 Features High performance in terms of accuracy and precision for flow and composition e High power range combining high pressure up to 1300 bar and high analytical flow rates up to 5 mL min for high chromatographic performance e Integrated high efficiency degasser with low internal volume is based on PTFE AF technology and has a fast change over of solvents for purging and priming the pump High solvent mixing efficiency before reaching the pump head due to the established Inlet Weaver technology e Active damping with independently controllable high resolution pump drives and firmware embedded tuning algorithms significantly reduce ripples and associated UV noise switchable V380 Jet Weaver for extra mixing volume e A multipurpose valve enables automatic software embedded functionalities such as switching the optional mixer in and out flushing back the inline filter or automatic purging The unique Intelligent System Emulation T
16. capillary connections from ports 1 3 and 6 Remove the clamp with the inline filter Use the 9 64 inch hex key for opening the valve head N Replace parts as required 3 Reassemble the valve head and mount it to the valve drive Bearing ring Rotor seal Stator ring Stator head Stator screws Agilent 1290 Infinity I Flexible Pump User Manual 135 7 Maintenance Replace the High Pressure Outlet Filter or Filter Frit Replace the High Pressure Outlet Filter or Filter Frit When For removing blockages and leaks in the high pressure filter assembly The outlet filter should be replaced as required depending on the system usage Other parts are covered by the Agilent Preventive Maintenance PM Service Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 1924 Wrench open 14 mm Torque wrench Torque wrench head 14 mm for torque wrench Parts required p n Description G4204 60004 Outlet filter 1290 Infinity Quaternary Pump 5067 5716 Frit for 1290 pump outlet filter 2 pk 1 Remove the capillary from the high pressure outlet filter 2 Remove the high pressure outlet filter from the filter to the pressure sensor 1 and from the high pressure holder outlet filter to the Multipurpose valve 2 136 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Replace the High Pressure Outlet Filter or Filter Frit 3 Unscrew the hi
17. for the appropriate IPiisettings a Xircom CreditCard Ethemet 10 100 Modem 56 C_Dbierrarrtatiireseautomatically Use the following IP address IP adiere 192 168 254 1 Subnet mask 255 255 248 0 Default gateway p Cc Install Uninstall Properties Ji ER Use the folo General Transmission Control Protocol Intemet Protocol The default pEenered ONS You can get IP settings assigned automatically if your network supports ie ne cali Dre is De ies connie Altemate DNS this capability Otherwise you need to ask your network administrator for the appropriate IP settings Show icon in taskbar when connected Obtain an IP address automatically C Use Wetoporine scares automatic IP address via DHCP tli I requires special IT setup of the network Obtain DNS server address automatically C Use the following DNS server addresses Figure 44 Changing the TCP IP settings of the PC 216 Agilent 1290 Infinity II Flexible Pump User Manual LAN Configuration 11 User Interface Software Setup Install you user interface software according the provided User Interface Software Setup Guide Agilent 1290 Infinity II Flexible Pump User Manual 217 11 LAN Configuration PC and User Interface Software Setup Setup 218 Agilent 1290 Infinity II Flexible Pump User Manual Agilent 1290 Infinity Il Flexible Pump User Manual 12 Appendix General Safety Information 220 General Safety Infor
18. from the Jet Weaver to the Multi Purpose Valve 2 Open the screw which fixes the Jet Weaver to the front panel Agilent 1290 Infinity I Flexible Pump User Manual 95 7 Maintenance Remove the Jet Weaver 3 Lift up the Jet Weaver 1 and pull it out of the front panel 4 If no other Jet Weaver shall be installed use plastic 2 fittings for closing unused ports of the valve and install the metal lid OR Otherwise continue at Install the Jet Weaver on page 97 Plastic fittings 96 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Install the Jet Weaver Install the Jet Weaver When The optional Jet Weaver 380 uL for 1290 Infinity Quaternary Pump G4204 68000 is available for applications which require highest mixing performance see chapter Optimizing Performance Tools required Description Screwdriver Pozidriv 1 Parts required p n Description 1 G4204 68000 Jet Weaver 380 uL for 1290 Infinity Quaternary Pump containing 2 5067 5416 Capillary ST 0 17 x 120 mm SLV SV Jet Weaver to Multi Purpose Valve Preparations Switch off the pump at the main power switch 1 Open the screw of the Jet Weaver metal lid 2 Remove the metal lid by lifting it up 1 and pulling it out of the front panel 2 Agilent 1290 Infinity I Flexible Pump User Manual 97 7 Maintenance Install the Jet Weaver 3 Insert the Jet Weaver to the
19. high resolution encoder unit is attached to the pump drives which divides a single turn into 65000 steps Each step corresponds to a volume of about 300 pL which allows an extremely precise control Agilent 1290 Infinity II Flexible Pump User Manual Introduction 1 Operating Principle Multisampler TE on ressure senso en Degasser Seal wash pump Multipurpose Inlet weaver Seal wash sensor Figure2 The hydraulic path Agilent 1290 Infinity I Flexible Pump User Manual 13 1 Introduction Positions of the Multi Purpose Valve Positions of the Multi Purpose Valve The Multi Purpose Valve allows easy software controlled switching between different modes of operation Normal Operating Mode Without Mixer In normal operating mode the flow comes from the pump head passes the pressure sensor and arrives at the central port of the Multi Purpose Valve The flow passes the inline filter and leaves the valve through port 4 to the system autosampler etc From pump To system Inline filter Figure 3 Valve position in normal operating mode without mixer 14 Agilent 1290 Infinity II Flexible Pump User Manual Introduction Positions of the Multi Purpose Valve Purge Mode In purge mode the flow is diverted to the waste container From pump To waste Figure 4 Valve position in purge mode Agilent 1290 Infinity I Flexible Pump User Manual 1 1 Introduction Positions of the Multi P
20. limit violation 77 deliver underrun 67 drive current too high 65 drive current too low 65 drive encoder error 70 drive encoder failed 76 drive phases differ too much in electric esistance 76 drive position limit 70 drive timeout 66 fan failed 59 flow rate limit exceeded 62 Agilent 1290 Infinity I Flexible Pump User Manual insufficient power of drive encoder LED 70 leak sensor open 57 leak sensor short 56 leak 59 lost CAN partner 56 maximum stroke too short 72 multi purpose valve failed 68 overcurrent of pump drive 66 pressure below lower limit 60 pressure exceeded upper pressure limit 60 pump drive blocked or encoder failed 64 pump drive encoder defect 68 pump drive encoder error 71 pump drive encoder rollover 69 pump drive error 72 pump drive stop not found 73 pump error 60 quaternary pump shutdown during analysis 63 reading of multi purpose valve tag failed 69 reading the pump encoder tag failed 63 remote timeout 55 seal wash pump was missing when tried to turnon 77 shutdown 54 solvent counter exceeded limit 61 target pressure not reached for quaternary pump degasser 61 timeout wait for composition 73 timeout wait for flow 75 timeout wait for pressure 75 timeout wait for run volume 74 timeout wait for volume 74 timeout 54 unknown multi purpose valve type 71 valve hardware overcurrent MCGV 78 waste counter limit exceeded 62 writing the multi purpose valve tag failed 71
21. main power switch Open the doors Use an optional solvent shutoff valve or lift up solvent filters inside solvent bottles for avoiding leakages For best performance and life time use lower channels A and D for aqueous solvents in buffer applications 1 Use the mounting tool for removing tubing connections between the degassing unit and the MCGV 2 Remove the inlet weaver see Replace the Inlet Weaver on page 90 3 Remove the cover from the MCGV 100 Agilent 1290 Infinity II Flexible Pump User Manual 7 Maintenance Replace the Multi Channel Gradient Valve MCGV 4 Disconnect the MCGV cable 1 unscrew the two screws 2 3 and remove the valve 5 Place the new MCGV into position NOTE Make sure that channel A of the MCGV is put at the bottom right position 6 Tighten the two screws 1 2 and connect the cable to its connector 3 Next Steps Install the inlet weaver see Replace the Inlet Weaver o 9 Reconnect solvent tubes for channels A D from the MCGV to the degasser outlets 7 Install the MCGV cover n page 90 Agilent 1290 Infinity I Flexible Pump User Manual 101 7 Maintenance Release a Stuck Inlet Valve Tools required p n Description 9301 0411 Syringe Plastic 0100 1681 Syringe adapter luer barb 0100 1710 Beaker CAUTION Mounting Tool for Tubing Connections Pressure damages the multi channel gradient va
22. marked with this symbol when hot surfaces are available and the user should not touch it when heated up gt Cooling unit is designed as vapor compression refrigeration system Contains fluorinated greenhouse gas refrigerant according to the Kyoto protocol For specifications of refrigerant charge capacity carbon dioxide equivalent CDE and global warming potential GWP see instrument label Confirms that a manufactured product complies with all applicable C European Community directives The European Declaration of Conformity is available at http regulations corporate agilent com DoC search htm j Manufacturing date Power symbol indicates On Off The apparatus is not completely disconnected from the mains supply when the power switch is in the Off position 224 Agilent 1290 Infinity II Flexible Pump User Manual Appendix 12 Table 17 Symbols Pacemaker A Magnets could affect the functioning of pacemakers and implanted heart defibrillators A pacemaker could switch into test mode and cause illness A heart defibrillator may stop working If you wear these devices keep at least 55 mm distance to magnets Warn others who wear these devices from getting too close to magnets Magnetic field A Magnets produce a far reaching strong magnetic field They could damage TVs and laptops computer hard drives credit and ATM cards data storage media mechanical watches hearing aids and speakers Keep ma
23. of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as Agilent 1290 Infinity II Flexible Pump User Manual Hardware Information 10 e standard TTL levels 0 V is logic true 5 0 V is false e fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or Lwhen between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Table6 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injectio
24. on again and establish the TCP IP connection using the new parameters When the module is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network the user may simply keep the default parameters to establish the TCP IP connection In the Using Default mode the parameters stored in the memory of the module are not cleared automatically If not changed by the user they are still available when switching back to the mode Using Stored Agilent 1290 Infinity II Flexible Pump User Manual LAN Configuration 11 Dynamic Host Configuration Protocol DHCP General Information DHCP The Dynamic Host Configuration Protocol DHCP is an auto configuration protocol used on IP networks The DHCP functionality is available on all Agilent HPLC modules with on board LAN Interface or LAN Interface Card and B firmware B 06 40 or above When the initialization mode DHCP is selected the card tries to download the parameters from a DHCP Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the card Besides requesting the network parameters the card also submits its hostname to the DHCP Server The hostname equals the MAC address of the card e g 0030d3177321 It is the DHCP server s responsibility to forward the hostname address information to the Domain Name Server The card does not offer any services for hos
25. peak capacity than a longer STM column by virtue of running it faster Refer to Petersson et al J Sep Sci 31 2346 2357 2008 Maximizing peak capacity and separation speed in liquid chromatography Agilent 1290 Infinity I Flexible Pump User Manual 45 4 How to Optimize the Performance of Your Module Using Solvent Calibration Tables 46 Importing Solvent Calibration Tables RC NET based Agilent graphical user interfaces ChemStation EZChrom Elite OpenLab etc include data for most commonly used solvents in HPLC This data contains solvent properties and is used for optimum pump control in order to ensure best flow and composition accuracy If your solvent is not included to the software please check the Agilent web site http www chem agilent com en US Support Downloads firmware Pages LC aspx for additional libraries registration required which also provides updates and optimized data If your solvent is neither available in the user interface nor in the library please use generic solvents Generic aequeous gives good results for most solvent mixtures with at least 50 water which have similar properties as pure water For other solvents with high organic percentage Generic organic gives a good approximation Importing Solvent Calibration in ChemStation 1 Go to menu Instrument gt Instrument configuration 2 In the Instrument Configuration screen choose your module and click Configure Click Configure
26. supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet gt Ground the waste container gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly gt Do not use solvents with an auto ignition temperature below 200 C 392 F Agilent 1290 Infinity II Flexible Pump User Manual 223 12 Appendix Safety Symbols Table 17 Symbols The apparatus is marked with this symbol when the user should refer to A the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage j Indicates dangerous voltages Indicates a protected ground terminal i The apparatus is
27. that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Leak Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries Agilent 1290 Infinity II Flexible Pump User Manual 59 6 Error Information Pump Error Messages These errors are pump specific Pressure of quaternary pump above upper limit Error ID 29163 The pressure has exceeded the upper pressure limit e Parameter Measured pressure Probable cause Suggested actions 1 Blockage in flow path after the pressure Check for blockages in the LC system e g purge sensor valve Jet Weaver degraded column column frits needle needle seat capillaries etc Check for particles in the solvent 2 Inappropriate settings pressure limit flow Decrease f
28. the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1290 Infinity II Flexible Pump User Manual In This Guide In This Guide This manual covers the Agilent 1290 Infinity II Flexible Pump G7104A 1 Introduction This chapter gives an introduction to the module instrument overview and internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Using the Pump This chapter explains the operational parameters of the Agilent 1290 Infinity II Flexible Pump 4 How to Optimize the Performance of Your Module This chapter gives hints on how to optimize the performance or use additional devices 5 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features 6 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 7 Maintenance This chapter describes the maintenance of the Agilent 1290 Infinity II Flexible Pump Agilent 1290 Infinity II Flexible Pump User Manual 3 In This Guide 8 Parts and Materials This chapter provides information on parts for maintenance and repair 9 Identifying C
29. the thermostat by a few degrees and again experiment will reveal the answer The van Deemter curve shows that the optimum flow rate through an STM column is higher than for larger particles and is fairly flat as the flow rate increases Typical close to optimum flow rates for STM columns are 2 ml min for 4 6 mm i d and 0 4 ml min for 2 1 mm i d columns In isocratic separations increasing the retention factor k results in better resolution because the solute is retained longer In gradient separations the retention is described by k in the following equation pefo F 100 A B V S m where r k mean k value tg time length of gradient or segment of gradient min Agilent 1290 Infinity II Flexible Pump User Manual How to Optimize the Performance of YourModule 4 F flow ml min Vin column delay volume e A B change in fraction of solvent B during the gradient e S constant ca 4 5 for small molecules This shows that k and hence resolution can be increased by having a shallower gradient 2 to 5 min change is a guideline higher flow rate and a smaller volume column This equation also shows how to speed up an existing gradient if the flow is doubled but the gradient time is halved k remains constant and the separation looks the same but happens in half the time Recently published research has shown how a shorter STM column at temperatures above 40 C can generate higher
30. to pump drive Probable cause 1 Defect connection between main board and pump drive encoder 2 Defect encoder Agilent 1290 Infinity II Flexible Pump User Manual Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative 67 6 6 68 Error Information Pump drive encoder defect Error ID 29209 Defect pump drive encoder e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Defect encoder Please contact your Agilent service representative Multi Purpose Valve failed Error ID 29231 Lost steps of the purge valve encoder Parameter None Probable cause Suggested actions 1 Multi purpose valve drive mechanically e Check installation of multi purpose valve blocked or defect head e Replace multi purpose valve head Agilent 1290 Infinity II Flexible Pump User Manual Error Information Reading of multi purpose valve tag failed Error ID 29240 Reading the multi purpose valve tag failed Parameter None Probable cause Suggested actions 1 Reading of multi purpose valve tag failed Check cable connection 2 Multi purpose valve head tag defect or Replace multi purpose valve head empty 3 Multi purpose valve tag reader is defect Please contact your Agilent service representative Pump drive encoder rollover Error ID 29232 Invalid pump drive encoder signals have been detected e Parameter 1
31. v ump head assembly disassemble 108 install 131 reassemble 130 remove 106 pump prepare for transport 141 Q quaternary pump shutdown during analysis 63 radio interference 227 reading of multi purpose valve tag failed 69 reading the pump encoder tag failed 63 remote cables 166 remove doors 87 jet weaver 95 pump head assembly 106 repairs replacing firmware 140 replace gasket 120 high pressure filter frit 136 high pressure filter 136 Agilent 1290 Infinity II Flexible Pump User Manual inlet valve 92 inlet weaver 90 MCGV 100 multi channel gradient valve 100 multi purpose valve 133 outlet valve 93 pressure sensor 88 seal wash pump 99 wash seal 120 resolution Optimization 43 RS 232C cable 171 S safety class 220 safety general information 220 standards 27 symbols 224 seal wash pump was missing when tried to turnon 77 seal wash pump replace 99 secondary pump head assemble 122 disassemble 114 parts 152 serial number information 178 shutdown 54 site requirements 23 power cords 25 solvent counter exceeded limit 61 special interfaces 183 special settings boot resident 186 forced cold start 186 specification physical 27 specifications 23 performance 28 T target pressure not reached for quaternary pump degasser 61 TCP IP parameter configuration 191 telnet configuration 212 temperature sensor 59 timeout wait for composition 73 timeout wait for flow 75 timeout w
32. 10 08 Figure 31 LAN Setting on Instant Pilot 2 Set the Configuration Switch to DHCP either on the G1369C LAN Interface Card or the main board of above mentioned modules Table 13 G1369C LAN Interface Card configuration switch on the card SW 4 SW 5 SW 6 SW7 SW 8 Initialization Mode ON OFF OFF OFF OFF DHCP 198 Agilent 1290 Infinity II Flexible Pump User Manual LAN Configuration 11 Table 14 LC Modules inclusive 1120 1220 configuration switch at rear of the instru ment SW6 SW7 SW 8 Initialization Mode ON OFF OFF DHCP 3 Turn on the module that hosts the LAN interface 4 Configure your Control Software e g Agilent ChemStation Lab Advisor Firmware Update Tool and use MAC address as host name e g 003043177321 The LC system should become visible in the control software see Note in section General Information DHCP on page 197 Agilent 1290 Infinity II Flexible Pump User Manual 199 11 LAN Configuration Link Configuration Selection The LAN interface supports 10 or 100 Mbps operation in full or half duplex modes In most cases full duplex is supported when the connecting network device such as a network switch or hub supports IEEE 802 3u auto negotiation specifications When connecting to network devices that do not support auto negotiation the LAN interface will configure itself for 10 or 100 Mbps half duplex operation For example when connected to a non negotiating 10 Mbps
33. 232 cable 8 m Agilent 1290 Infinity II Flexible Pump User Manual 171 9 Identifying Cables USB Cables To connect a USB Flash Drive use a USB OTG cable with Mini B plug and A socket p n Description 5188 8050 USB A M USB Mini B 3 m PC Module 5188 8049 USB A F USB Mini B M OTG Module to Flash Drive Agilent 1290 Infinity II Flexible Pump User Manual Agilent 1290 Infinity Il Flexible Pump User Manual 10 Hardware Information Firmware Description 174 Electrical Connections 177 Serial Number Information 178 Rear View of the Module 178 Interfaces 179 Overview Interfaces 180 Setting the 8 bit Configuration Switch 184 Special Settings 186 Early Maintenance Feedback 187 Instrument Layout 188 This chapter describes the pump in more detail on hardware and electronics ot Agilent Technologies 173 10 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections a non instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are the complete communication capabilities CAN LAN USB and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN USB and RS 2
34. 32C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote error handling diagnostic functions e or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 174 Agilent 1290 Infinity II Flexible Pump User Manual Hardware Information 10 Firmware Updates Firmware updates can be done using the following tools latest version should be used Agilent Lab Advisor software with files on the hard disk Firmware Update Tool with local files on the hard disk Instant Pilot G4208A with files on a USB Flash Disk Required tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315B for the G1315B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 650 is revision 6 50 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done
35. 5188 8044 ERI to ERI Connector D_Subminiature 15 pin 166 Agilent 1290 Infinity II Flexible Pump User Manual Table 3 5188 8044 ERI to ERI Identifying Cables 9 p n 5188 8044 Pin ERI Signal Pin ERI o 10 GND 10 m 2 1 Start Request 1 2 Stop 2 3 Ready 3 5 Power on 5 4 Future 6 Shut Down 6 7 Start 7 8 Prepare 8 Ground Cable Shielding NC Connection 5188 8045 ERI to APG Connector D_Subminiature 15 pin ERD Connector D_Subminiature 9 pin APG p n 5188 8045 Pin ERI Signal Pin APG 10 GND 1 7 1 Start Request 9 2 Stop 8 3 Ready 7 5 Power on 6 4 Future 5 6 Shut Down 4 7 Start 3 8 Prepare 2 Ground Cable Shielding NC Connection 167 Agilent 1290 Infinity II Flexible Pump User Manual 9 168 Identifying Cables One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent Signal Name Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent 1290 Infinity II Flexible Pump
36. 86 Remove and Install Doors 87 Replace the Pressure Sensor 88 Replace the Inlet Weaver 90 Replace the Inlet Valve 92 Replace the Outlet Valve 93 Remove the Jet Weaver 95 Install the Jet Weaver 97 Replace the Seal Wash Pump 99 Replace the Multi Channel Gradient Valve MCGV 100 Release a Stuck Inlet Valve 102 Pump Head Procedures 104 Replace the Multi Purpose Valve 133 Replace Parts of the Multi Purpose Valve 135 Replace the High Pressure Outlet Filter or Filter Frit 136 Replace Parts of the Inline Filter 138 Replace the Module Firmware 140 Prepare the Pump Module for Transport 141 Parts and Materials 143 Overview of Main Assemblies 144 Flow Connections 146 Pump Head Assembly Parts 148 Primary Pump Head Parts 150 Secondary Pump Head Parts 152 Multi Purpose Valve 154 Cover Parts 155 Accessory Kit 156 Tool Kit 157 Tools 158 Agilent 1290 Infinity II Flexible Pump User Manual Contents 9 Identifying Cables 161 Cable Overview 162 Analog Cables 164 Remote Cables 166 CAN LAN Cables 170 Agilent Module to PC 171 USB Cables 172 10 Hardware Information 173 Firmware Description 174 Electrical Connections 177 Interfaces 179 Setting the 8 bit Configuration Switch 184 Early Maintenance Feedback 187 Instrument Layout 188 1 LAN Configuration 189 What You Have To Do First 190 TCP IP Parameter Configuration 191 Configuration Switch 192 Initialization Mode Selection 193 Dynamic Host Configuration
37. 90 Infinity I Flexible Pump User Manual 117 7 Maintenance Pump Head Procedures 3 Remove the 2 5 mm hex screw at the bottom of the pump head Do not drop the golden spacer fitting Hex screw 4 Lift out the heat exchanger 5 If removed first insert the spacer fitting Then insert the new heat exchanger to the opening in the pump head and lift it over the pins _ ee Spacer fitting 6 Use the 19 mm screw for fixing the front plate Front plate 118 Agilent 1290 Infinity I Flexible Pump User Manual Maintenance 7 Pump Head Procedures 7 Insert and fix the 2 5 mm hex screw Hex screw is Agilent 1290 Infinity I Flexible Pump User Manual 119 7 Maintenance Replace Wash Seal and Gasket 1 To remove the wash seal use the steel side of the insert tool Wash seal 2 Use the soft plastic side of the insert tool for inserting the wash seal into the support ring amp Insert tool Wash seal Support inog Clean seals before Reinstallation or use new ones Store seals in isopropanol bath before installation 120 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 3 Put the gasket into the support ring and insert the seal holder Distance sheet Seal holder Gasket Support ring Lor Agilent 1290 Infinity I Flexible Pump User Manual 121 7 Maintenance Assemble the Primary a
38. B A F USB Mini B M OTG Module to Flash Drive Agilent 1290 Infinity II Flexible Pump User Manual 163 9 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 35900 A D converters p n 35900 60750 35900 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog 164 Agilent 1290 Infinity II Flexible Pump User Manual Identifying Cables 9 Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module yoo la Shield Shield Analog pa o T Ne Center Center Analog Agilent Module to General Purpose p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog _Se 3 Red Analog S Agilent 1290 Infinity II Flexible Pump User Manual 165 9 Identifying Cables Remote Cables ERI Enhanced Remote Interface 5188 8029 ERI to general purpose Classic Remote p n 5188 8029 pin Enhanced Remote 1 101 D Sub female 15way user s view to connector 2 102 2egaeege8 3 103 C 104 150 0 0000 e ie 2090 084 NN ODOunD 2222s zZ g 6 106 S 7 107 8 108 9 1wire DATA 10 DGND 11 5V ERI out 12 PGND 13 PGND 14 24V ERI out 15 24V ERI out START REQUEST STOP READY POWER ON NOT USED SHUT DOWN START PREPARE
39. B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler 180 Agilent 1290 Infinity II Flexible Pump User Manual Hardware Information 10 RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for e 19200 baud 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table5 RS 232C Connection Table Pin Direc
40. C grade solvents and water filtered through 0 2 um filters e Label bottles correctly with bottle content and filling date expiry date Use solvent inlet filters Reduce risk of algae growth use brown bottles for aqueous solvents avoid direct sunlight Select channels for Multi Channel Gradient Valve MCGV Use lower channels A and or D for buffer solutions e Regularly flush all MCGV channels with water to remove possible salt deposits e Check compatibility of buffers and organic solvents to avoid precipitation Agilent 1290 Infinity II Flexible Pump User Manual 37 3 Using the Pump Best Practices 38 Agilent 1290 Infinity II Flexible Pump User Manual Agilent 1290 Infinity Il Flexible Pump User Manual 4 How to Optimize the Performance of Your Module Delay Volume and Extra Column Volume 40 Delay Volume 40 How to Configure the Optimum Delay Volume 41 How to Achieve Higher Resolution 43 Using Solvent Calibration Tables 46 This chapter gives hints on how to optimize the performance or use additional devices ott Agilent Technologies 39 4 How to Optimize the Performance of Your Module Delay Volume and Extra Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the top of the column The extra column volume is defined as the volume between the injection point and the detection point excluding the volume in the column Delay Volume In grad
41. Protocol DHCP 197 Link Configuration Selection 200 Automatic configuration with Bootp 201 Manual Configuration 211 PC and User Interface Software Setup Setup 216 12 Appendix 219 General Safety Information 220 Waste Electrical and Electronic Equipment Directive 226 Radio Interference 227 Sound Emission 228 Agilent Technologies on Internet 229 Agilent 1290 Infinity II Flexible Pump User Manual Contents 8 Agilent 1290 Infinity I Flexible Pump User Manual Agilent 1290 Infinity Il Flexible Pump User Manual 1 Introduction Product Description 10 Features 11 Operating Principle 12 Positions of the Multi Purpose Valve 14 Leak and Waste Handling 19 Waste Concept 21 This chapter gives an introduction to the module instrument overview and internal connectors Apg Agilent Technologies 1 introduction Product Description The Agilent 1290 Infinity II Flexible Pump improves your efficiency by combining the performance of a high pressure mixing UHPLC pump with the flexibility of a low pressure mixing UHPLC pump The new 1290 Infinity II LC power range has a high instrument efficiency allowing you to run any HPLC and UHPLC method ISET enables you to transfer existing methods from different instrument modules current Agilent systems as well as instruments from other manufacturers The Agilent Inlet Weaver mixer active damping or the optional Agilent V380 Jet Weaver mixer for additional mixing capacity achieve high
42. Tool for Tubing Connections Syringe adapter luer barb Insert tool Blank Nut SL Agilent 1290 Infinity II Flexible Pump User Manual 157 8 Parts and Materials Tools Item p n Description 5067 4699 1290 Infinity pump service kit 1 5067 5688 Torque wrench 1 25 Nm with 14 mm wrench 2 G4220 20013 4 mm hex bit 3 G4220 20014 2 5 mm Hex Bit 4 G4220 20015 Adapter in square to hex 5067 5691 Wrench open 19 mm G4220 20041 Bit Torx 10x25 mm 5023 0285 Replacement kit for 1290 Infinity pump head alignment tool piston handle 1290 Infinity pump service kit 5067 4699 includes pump head alignment tool and items 1 4 Figure 16 Pump head alignment tool 158 Agilent 1290 Infinity II Flexible Pump User Manual Parts and Materials 8 Tools Alignment piston Tool handle Figure 17 Replacement kit for 1290 Infinity pump head alignment tool piston handle Figure 18 HPLC System Tool Kit Infinity II Agilent 1290 Infinity I Flexible Pump User Manual 159 8 Parts and Materials Tools 160 Agilent 1290 Infinity II Flexible Pump User Manual This chapter provides information on cables used with the modules Agilent 1290 Infinity Il Flexible Pump User Manual 9 Identifying Cables Cable Overview 162 Analog Cables 164 Remote Cables 166 CAN LAN Cables 170 Agilent Module to PC 171 USB Cables 172 Apg Agilent Technologies 161 9 Identifying Cables Cable Overview Never use cables other than the ones sup
43. User Manual Agilent Module to General Purpose Identifying Cables 9 p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL 7 White 1 Digital ground a Ol ou Brown 2 Prepare run Low 00 pes DE KEY Gray 3 Start Low oo eee S S te Blue 4 Shut down Low og Pink 5 Not oa connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low Agilent 1290 Infinity II Flexible Pump User Manual 169 9 Identifying Cables CAN LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection 170 Agilent 1290 Infinity II Flexible Pump User Manual Identifying Cables 9 Agilent Module to PC p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS
44. ables This chapter provides information on cables used with the modules 10 Hardware Information This chapter describes the pump in more detail on hardware and electronics 11 LAN Configuration This chapter provides information on connecting the module to the controller software 12 Appendix This chapter provides additional information on safety legal and web 4 Agilent 1290 Infinity I Flexible Pump User Manual Contents Contents 1 Introduction 9 Product Description 10 Features 11 Operating Principle 12 Positions of the Multi Purpose Valve 14 Leak and Waste Handling 19 2 Site Requirements and Specifications 23 Site Requirements 24 Physical Specifications 27 Performance Specifications 28 3 Usingthe Pump 31 Magnets 32 Turn on off 33 Status Indicators 34 Best Practices 35 4 How to Optimize the Performance of Your Module 39 Delay Volume and Extra Column Volume 40 How to Configure the Optimum Delay Volume 41 How to Achieve Higher Resolution 43 Using Solvent Calibration Tables 46 5 Troubleshooting and Diagnostics 47 User Interfaces 48 Agilent Lab Advisor Software 49 6 Error Information 51 What Are Error Messages 53 General Error Messages 54 Pump Error Messages 60 Agilent 1290 Infinity II Flexible Pump User Manual Contents 7 Maintenance 79 Introduction to Maintenance 81 Warnings and Cautions 82 Overview of Maintenance 84 Cleaning the Module 85 Install Fittings and Capillaries
45. ad The default location for the logfile is C Documents and Settings All Users Application Data Agilent BootP LogFile The logfile will not be updated if it is open The contents will be similar to the following 02 25 10 15 30 49 PM Status BootP Request received at outermost layer Status BootP Request received from hardware address 0010835675AC Error Hardware address not found in BootPTAB 0010835675AC Status BootP Request finished processing at outermost layer 3 Record the hardware MAC address for example 0010835675AC Agilent 1290 Infinity II Flexible Pump User Manual LAN Configuration 11 4 The Error means the MAC address has not been assigned an IP address and the Tab File does not have this entry The MAC address is saved to the Tab File when an IP address is assigned 5 Close the log file before turning on another instrument 6 Uncheck the Do you want to log BootP requests box after configuring instruments to avoid having the logfile use up excessive disk space Adding each instrument to the network using BootP 1 Follow Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings screen appears 2 Uncheck the Do you want to log BootP requests once all instruments have been added The Do you want to log BootP requests box must be unchecked when you have finished configuring instruments otherwise the log file will quickly fill up disk space 3 Click Edit Boo
46. ad the required module firmware the latest LAN USB FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Prepare the Pump Module for Transport When If the module shall be transported or shipped Parts required p n Description 9301 0411 Syringe Plastic 9301 1337 Syringe adapter G7104 44000 Transport protection foam CAUTION Mechanical damage gt For shipping the module insert the Protective Foam to protected the module from mechanical damage gt Be careful not to damage tubing or capillary connections while inserting the module in the Protective Foam 1 Flush all solvent channels with isopropanol 8 Disconnect the degasser outlet tubings at the MCGV one after another Use a syringe for removing liquid from the degasser and the solvent tubings 2 Remove solvent inlet tubes from solvent reservoirs and tubing clips at other modules 3 Remove tubings between the seal wash function and solvent bottle waste 4 Turn off the pump Remove cable and capillary connections to other modules Remove the waste tube 7 Remove the module from the stack Agilent 1290 Infinity I Flexi
47. address it will be necessary to power cycle the instrument for the changes to take effect 5 Leave Edit BootP Addresses by pressing Close 6 Exit BootP Settings by pressing OK Restarting the Agilent BootP Service 1 In the Windows control panel select Administrative Tools gt Services The Services screen appears see Figure 35 on page 209 2 Right click Agilent BootP Service and select Start 3 Close the Services and Administrative Tools screens 210 Agilent 1290 Infinity II Flexible Pump User Manual LAN Configuration 11 Manual Configuration Manual configuration only alters the set of parameters stored in the non volatile memory of the module It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters given that the initialization mode selection switches are allowing it Non Volatile RAM Stored Parameter Ld Control Module Figure 37 Manual Configuration Principle Agilent 1290 Infinity II Flexible Pump User Manual 211 11 LAN Configuration With Telnet Whenever a TCP IP connection to the module is possible TCP IP parameters set by any method the parameters may be altered by opening a Telnet session 1 2 212 Open the system DOS prompt window by clicking on Windows START button and select Run Type cmd and press OK Type the follo
48. ait for pressure 75 timeout wait for run volume 74 timeout wait for volume 74 timeout 54 transport pump 141 troubleshooting error messages 53 U unknown multi purpose valve type 71 V valve hardware overcurrent MCGV 78 valve position filter flush mode 17 normal operating mode with mixer 16 normal operating mode without mixer 14 purge mode 15 service mode 18 valve replace 133 voltage range 27 Agilent 1290 Infinity I Flexible Pump User Manual Index Ww wash seal replace 120 waste counter limit exceeded 62 waste electrical and electronic equipment 226 WEEE directive 226 weight 27 writing the multi purpose valve tag failed 71 writing the pump encoder tag failed 64 233 www agilent com In This Book This manual contains technical reference information about the Agilent 1290 Infinity II Flexible Pump G7104A The manual describes the following Introduction e Site requirements and specifications using and optimizing e troubleshooting and diagnostic e error information test functions e maintenance e parts identification hardware information e safety and related information Agilent Technologies 2014 Printed in Germany 12 2014 G7104 90000 Rev B fe Agilent Technologies
49. analysis where required Shutdown Error ID 0063 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message 54 Agilent 1290 Infinity II Flexible Pump User Manual Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system Remote Timeout Error ID 0070 Error Information 6 Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Ag
50. apillary connections from the Multi Purpose Valve 1 Remove the clamp with the inline filter 2 Unscrew the black union nut and remove the head of the purge valve by pulling it to the front Agilent 1290 Infinity I Flexible Pump User Manual 133 7 Maintenance Replace the Multi Purpose Valve 3 Put the new valve head onto the valve drive such that the lobe fits to the groove Screw the valve head onto the valve drive using the union nut The central C port is connected to the outlet of the pressure sensor Port 1 is Port 2 is Port 3 is Port 4 is Port 5 is Port 6 is Port 7 is Port 8 is connected to the outlet of the optional Jet Weaver connected to the inlet of the optional Jet Weaver blocked by a blank nut plastic connected to the system typically multisampler connected to the outlet of the inline filter blocked by a blank nut SST connected to the waste capillary connected to the inlet of the inline filter Block unused ports with blank nuts 134 Agilent 1290 Infinity I Flexible Pump User Manual Maintenance 7 lace Parts of the Multi Purpose Valve Replace Parts of the Multi Purpose Valve Tools required p n Description 8710 2394 9 64 inch hex key Parts required p n Description 1535 4045 Bearing ring 5068 0202 Rotor seal Multi Purpose Valve 1300 bar 5068 0120 Stator ring 5068 0001 Stator head 1535 4857 Stator screws 10 Pk Preparations e Remove
51. are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or
52. ary Pump offers a strongly decreased delay volume compared to standard 600 bar pressure pumps For the 1290 Infinity Quaternary Pump mixing is done in the multi channel gradient valve at ambient pressure As all pump parts in the flow path after mixing contribute to the delay volume this includes also pump heads of the quaternary pump flow connections filters mixers etc Therefore the delay volume of a quaternary pump is by design larger than that of a binary pump All listed components including the inlet weaver and pump heads ensure a good mixing performance resulting in excellent composition precision and accuracy highly reproducible retention times and low baseline noise This ensures best results for most applications Per default the 1290 Infinity Quaternary Pump does not require and include a Jet Weaver as solvents are mixed in the MCGV and mixing is further improved in the inlet weaver pump heads and subsequent parts in the flow path Therefore no Jet Weaver is required for most applications The V380 Jet Weaver high performance mixer is optionally available for demanding applications which use solvents in different channels for example A versus B that differ strongly in their UV Vis absorption for example by using trifluoroacetic acid TFA as a modifier which has a high absorbance Solvent packages created by the pump may persist until the solvent reaches the detector flow cell Absorption fluctuations can then show up as
53. ation with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1290 Infinity II Flexible Pump User Manual 177 10 Hardware Information Electrical Connections Serial Number Information The serial number information on the instrument labels provide the following information CCYWWSSSSS Format CC country of manufacturing DE Germany JP Japan CN China YWW year and week of last major manufacturing change e g 820 could be week 20 of 1998 or 2008 SSSSS real serial number Rear View of the Module APG Remote Power supply for extern CAN valves Configuration switch CAN Bus Power socket Figure 20 Rear v
54. baseline noise also referred to as mixing noise Applications like impurity quantitation or lowest level compound detection require minimizing this noise The V380 Jet Weaver strongly improves mixing and therefore reduces baseline noise and improves sensitivity in detection Patented Agilent microfluidic technology offers high mixing performance at a low internal volume of 380 uL which is the physical volume of all channels It contributes with 150 uL to the pump delay volume lt 350 uL without Jet Weaver which is the partial mixer volume that creates a composition change corresponding to the delay volume Agilent 1290 Infinity II Flexible Pump User Manual 41 4 How to Optimize the Performance of Your Module How to Configure the Optimum Delay Volume Figure9 The Jet Weaver mixer The installation procedure is illustrated in Install the Jet Weaver on page 97 42 Agilent 1290 Infinity II Flexible Pump User Manual How to Optimize the Performance of YourModule 4 How to Achieve Higher Resolution Increased resolution in a separation will improve the qualitative and quantitative data analysis allow more peaks to be separated or offer further scope for speeding up the separation This section explains how resolution can be increased by examining the following points e Optimize selectivity e Smaller particle size packing e Longer Columns e Shallower gradients faster flow Resolution between two peaks is described by the res
55. ble Pump User Manual 141 7 Maintenance Prepare the Pump Module for Transport 9 Reconnect the degasser outlet tubings to the MCGV Remove the degasser inlet tubings 10 You may keep internal tubing and capillary connections Pressure sensor High pressure outlet filter Pump heads Inline filter 11 Carefully insert the protective foam to the front part of the instrument Do not damage any tubing or capillary connections Next Steps 12 Close the doors 13 For transport or shipment put the module and accessory kit to the original shipment box 142 Agilent 1290 Infinity I Flexible Pump User Manual This chapter provides information on parts for maintenance and repair Agilent 1290 Infinity Il Flexible Pump User Manual 8 Parts and Materials Overview of Main Assemblies 144 Flow Connections 146 Pump Head Assembly Parts 148 Primary Pump Head Parts 150 Secondary Pump Head Parts 152 Multi Purpose Valve 154 Cover Parts 155 Accessory Kit 156 Tool Kit 157 Tools 158 Apg Agilent Technologies 143 8 Parts and Materials Overview of Main Assemblies Overview of Main Assemblies 11 ar om N wo oo wl A Figure 11 Overview of main assemblies 144 Agilent 1290 Infinity II Flexible Pump User Manual Item p n 1 5067 5407 5023 0271 G4204 40000 2 5067 4237 3 G4204 60022 4 G4204 81090 5 G1311 67701 5041 8365 6 G4204 60600 7 G4220 60028 8 5065 4445
56. ce starts automatically when your PC reboots To change Agilent BootP Service settings you must stop the service make the changes and then restart the service Stopping the Agilent BootP Service 1 From the Windows control panel select Administrative Tools gt Services The Services screen appears RE Be aton yew teb s B iDRBI r gt r gt s n istes Beadgound inteky Cg c e Bo lectrtated unk Tra Cy etrtated Transa aa oe 2S Figure 35 Windows Services screen 2 Right click Agilent BootP Service 3 Select Stop 4 Close the Services and Administrative Tools screen Editing the IP address and other parameters in EditBootPSettings 1 Select Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings screen appears 2 When the BootP Settings screen is first opened it shows the default settings from installation Agilent 1290 Infinity I Flexible Pump User Manual 209 11 LAN Configuration 3 Press Edit BootP Addresses to edit the Tab File x 006000111893 Agrit 101 110 Agiert LEI night 255 255 2550 00 00 005000222803 Agler iC2 101 1 1 102 Agwat LCb 25525250 0000 Figure 36 Edit BootP Adresses screen 4 In the Edit BootP Addresses screen press Add to create a new entry or select an existing line from the table and press Modify or Delete to change the IP address comment subnet mask for example in the Tab File If you change the IP
57. condary Pump Head Parts Secondary Pump Head Parts Figure 14 Secondary pump head parts 152 Agilent 1290 Infinity II Flexible Pump User Manual Parts and Materials 8 Secondary Head G4204 60061 Item p n Description 1 0515 1218 Screw M5 40 mm long 2 G4220 20003 Pump Head Screw 3 G4220 20000 LID 4 G4220 81013 Heat Exchanger 5 G2404 25213 PH Body sec G4220 20028 Headless screw for 1290 Infinity pump heads not shown G4220 20001 Spacer Fitting not shown 6 0905 1719 Metering seal 100 pL 7 5023 2513 Distance sheet 8 G4220 60016 Seal holder including backup ring 9 01018 07102 Gasket Seal wash 10 0905 1718 Backup Seal PE Seal Wash 11 G4220 63010 Support Ring Seal Wash 12 G4220 23705 Bracket 13 G4220 60000 Preload Assy 14 5067 5938 Plunger Agilent 1290 Infinity II Flexible Pump User Manual 153 8 Parts and Materials Multi Purpose Valve Multi Purpose Valve Figure 15 Item p n 1 1535 4857 2 5068 0001 3 5068 0120 4 5068 0202 5 1535 4045 154 eS Multi Purpose Valve parts Description Stator screws 10 Pk Stator head Stator ring Rotor seal Multi Purpose Valve 1300 bar Bearing ring Agilent 1290 Infinity II Flexible Pump User Manual Cover Parts p n G4224 60200 G4224 60201 5043 0286 5067 5908 5067 5767 5067 5768 Parts and Materials Description Side Cover Right 200 Side Cover Left 200 Base Cover Top Cover Door assy 200 left IF II Door assy 200 right IF II Agilent 1290 Inf
58. dule incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 188 Agilent 1290 Infinity II Flexible Pump User Manual Agilent 1290 Infinity Il Flexible Pump User Manual 11 LAN Configuration What You Have To Do First 190 TCP IP Parameter Configuration 191 Configuration Switch 192 Initialization Mode Selection 193 Dynamic Host Configuration Protocol DHCP 197 General Information DHCP 197 Setup DHCP 198 Link Configuration Selection 200 Automatic configuration with Bootp 201 About Agilent Boo
59. e but didn t Either the limit time frame or the current value of the variable has been modified later directly or indirectly for example the flow rate Probable cause Suggested actions 1 Incorrect parameters have been senttothe Verify control software macros manual instrument by the control software or commands manual changes Timeout Wait for Volume Error ID 29182 A target condition volume which is the delivered flow since the limit has been set has been sent to the instrument which should have been reached within an expected time frame but didn t Either the limit time frame or the current value of the variable has been modified later directly or indirectly for example the flow rate Probable cause Suggested actions 1 Incorrect parameters have been senttothe Verify control software macros manual instrument by the control software or commands manual changes Agilent 1290 Infinity II Flexible Pump User Manual Error Information 6 Timeout Wait for Flow Error ID 29183 A target condition flow rate has been sent to the instrument which should have been reached within an expected time frame but didn t Either the limit time frame or the current value of the variable has been modified later directly or indirectly Probable cause Suggested actions 1 Incorrect parameters have been senttothe Verify control software macros manual instrument by the control software or commands manual changes Time
60. e module gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides 82 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment Agilent 1290 Infinity II Flexible Pump User Manual 83 7 Maintenance Overview of Maintenance The following pages describe maintenance simple repairs of the module that can be carried out without opening the main cover 84 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module
61. e style parameter value for example ip 134 40 27 230 Then press Enter where parameter refers to the configuration parameter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carriage return 5 Use the and press Enter to list the current settings Agilent 1290 Infinity II Flexible Pump User Manual 213 11 LAN Configuration E C AWINDOWS system32 cmd exe telnet 134 40 27 95 S LAN Status Page TCP IP Properties active IP Address Subnet Mask Def Gateway TCP IP Statu Figure 41 information about the LAN interface MAC address initialization mode Initialization mode is Using Stored active TCP IP settings TCP IP status here ready connected to PC with controller software e g Agilent ChemStation here not connected 134 40 27 95 255 255 248 0 134 400 24 1 no connections Telnet Current settings in Using Stored mode 6 Change the IP address in this example 134 40 27 99 and type to list current settings WINDOWS system32 cmd_exe telnet 134 40 27 95 gt ip 134 40 27 99 gt 4 LAN Status Page change of IP setting to Initialization mode is Using Stored active TCP IP settings 9838D36N8838 stored TCP IP settings in non volatile memory ing Stored ZIP Properties active IP Address Subnet Mask Def Gateway stored IP Address Subnet Mask Def Gateway
62. earing forces from the side gt Do not try to remove the pump piston from the rear gt Do not use the piston for removing pump seals Procedure is valid for primary and secondary pump head You may assemble pump head parts only correctly If parts are in wrong orientation the screws do not fit in Seals Always orientate the seals with the spring side towards the part Store seals in isopropanol and fit seals in wet Always ultrasonicate seals in solvent to clean seals properly Obey the following order to clean seals properly 1 H30 tab 2 H30 dest 3 H20 lsopropanol 50 50 4 isopropanol 100 If the pump head has been initialized without pump this may have damaged the pump drive In this case please contact your local Agilent service organisation Agilent 1290 Infinity II Flexible Pump User Manual 105 7 Maintenance Pump Head Procedures Remove the Pump Head Assembly Tools required p n G4220 20012 G4220 20013 G4220 20041 8710 0510 Description 4 mm hex bit Preparations Remove the pump from the stack Torque wrench 2 25 Nm Bit Torx 10x25 mm Wrench open 1 4 5 16 inch 1 In Lab Advisor go to Tools gt Remove Install Pump Head and follow instructions given on the screen 2 Open the doors 3 Remove the seal wash tubes 4 DO NOT REMOVE the capillary connection between the pump heads marked by the red X 5 Remove the capillary connection from the outlet f
63. eat exchanger 2 Drive motor defect Please contact your Agilent service representative Agilent 1290 Infinity II Flexible Pump User Manual 65 6 Error Information Drive timeout Error ID 29204 Movement of drive during initialization is blocked mechanically e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Blockage in flow path Remove capillary connection to system check outlet filter check valves check pump head 2 Blockage of pump drive Drive motor defect Please contact your Agilent service representative Overcurrent of pump drive Error ID 29202 The current consumption of the pump drive is too high e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Blockage of system before pressure sensor Check for blockage of e g outlet valve filter frit Multi Purpose Valve heat exchanger 2 Drive motor defect Please contact your Agilent service representative 66 Agilent 1290 Infinity II Flexible Pump User Manual Deliver underrun Error ID 29233 Internal error e Parameter None Probable cause 1 Internal error 2 Firmware issue Error Information Suggested actions Please contact your Agilent service representative Use a minimum firmware revision of B 06 55 Defect connection between main board and pump drive encoder Error ID 29208 Defect connection between main board and pump drive encoder e Parameter 1 2 referring
64. echnology SET enables the emulation of existing Agilent 1100 1200 and 1260 Infinity Series instruments as well as Waters Alliance Waters H Class and Shimadzu Prominence instruments e Precise and accurate buffer additive blending using the new software feature BlendAssist implemented in the pump driver Built in active seal wash for increased uptime Agilent 1290 Infinity II Flexible Pump User Manual 11 1 Introduction Operating Principle 12 The pump head comprises two pump chambers in series with independent high resolution motion control A pressure sensor in the flow path monitors the pressure The pump control uses this signal for minimizing the pressure ripple in order to achieve highest flow precision A stable flow can be delivered even in case of eventual small internal leaks which can be compensated automatically A heat exchanger between two pump chambers strongly reduces thermal effects due to solvent compression under very high pressures As solvents are compressed by the pump head and expand further down the flow path for example in the column the volumetric flow is changed depending on the compressibility of the liquid Agilent control software allows specifying pure solvents pre mixed solvents and solvent gradients Associated Agilent solvent libraries are used by the pump control for enhanced flow accuracy which is required for cross instrument or cross system reproducibility and method compatibility A
65. ed to a line voltage higher than specified gt Connect your instrument to the specified line voltage only The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 24 Agilent 1290 Infinity II Flexible Pump User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outl
66. emove salt deposits Visually inspect solvent filters Clean or exchange if necessary Power up Shut down the pump Power up the pump e Use new or different mobile phase as required e Purge pump heads with 2 5 3 mL min for 5 min Condition pump heads for 10 20 min Long term shut down of the pump Flush system with water to remove buffer Use recommended solvents to store the system e Power off the pump or system Agilent 1290 Infinity II Flexible Pump User Manual 35 3 Using the Pump Prepare CAUTION the pump Purge Use the Purge function to e fill the pump exchange a solvent remove air bubbles in tubes and pump heads Condition Use the Conditioning function e daily when starting the pump to minimize pressure ripple by dissolving air bubbles in pump heads Condition your complete system with solvents and composition of your application for example 50 50 A B at 0 5 mL min Seal wash The seal wash function runs continuously and is controlled by the seal wash sensor This guarantees a maximum seal life time Contaminated seal wash solvent gt Do not recycle seal wash solvent to avoid contamination gt Weekly exchange seal wash solvent 36 Agilent 1290 Infinity II Flexible Pump User Manual Using the Pump 3 How to deal with solvents e Use clean bottles only e Exchange water based solvents daily Select solvent volume to be used up within 1 2 days Use only HPL
67. entral administration of IP addresses for Agilent instruments on a LAN The BootP service must be running TCP IP network protocol and cannot run a DHCP server Agilent 1290 Infinity II Flexible Pump User Manual 201 11 LAN Configuration 202 How BootP Service Works When an instrument is powered on an LAN Interface in the instrument broadcasts a request for an IP address or host name and provides its hardware MAC address as an identifier The Agilent BootP Service answers this request and passes a previously defined IP address and host name associated with the hardware MAC address to the requesting instrument The instrument receives its IP address and host name and maintains the IP address as long as it is powered on Powering down the instrument causes it to lose its IP address so the Agilent BootP Service must be running every time the instrument powers up If the Agilent BootP Service runs in the background the instrument will receive its IP address on power up The Agilent LAN Interface can be set to store the IP address and will not lose the IP address if power cycled Situation Cannot Establish LAN Communication If a LAN communication with BootP service cannot be established check the following on the PC e Is the BootP service started During installation of BootP the service is not started automatically Does the Firewall block the BootP service Add the BootP service as an exception e Is the LAN Interface usin
68. ept overview typical stack configuration as an example Agilent 1290 Infinity II Flexible Pump User Manual 19 1 20 Introduction The solvent cabinet is designed to store a maximum volume of 6 L solvent The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 4 L For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets a printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet All leak plane outlets are situated in a consistent position so that all Infinity and Infinity II modules can be stacked on top of each other Waste tubes are guided through a channel on the right hand side of the instrument keeping the front access clear from tubes The leak plane provides leak management by catching all internal liquid leaks guiding them to the leak sensor for leak detection and passing them on to the next module below if the leak sensor fails The leak sensor in the leak plane stops the running system as soon as the leak detection level is reached Solvent and condensate is guided through the waste channel into the waste container e from the detector s flow cell outlet from the Multisampler needle wash port e from the Sample Cooler condensate e from the Seal Wash Sensor e from the pump s Purge Valve or Multipurpose Valve The waste tube connected to the leak pan outlet on each of the bott
69. er Multi Channel Gradient Valve MCGV Inlet Weaver Pump Head Assembly Pressure Sensor Multi Purpose Valve Inline Filter Figure 10 Overview of maintenance parts Recommended Interval for Preventive Maintenance The recommended interval for preventive maintenance is e 100 L or 1 year whichever comes first This recommendation is valid for LC instruments on which typical applications are running A typical application can be characterized as follows e pressure range 100 800 bar e flow rates 0 5 3 5 mL min e typical solvents used in reversed phase LC Agilent 1290 Infinity II Flexible Pump User Manual 81 7 Maintenance Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of th
70. es Agilent 1290 Infinity II Flexible Pump User Manual 49 5 Troubleshooting and Diagnostics Agilent Lab Advisor Software 50 Agilent 1290 Infinity II Flexible Pump User Manual Agilent 1290 Infinity Il Flexible Pump User Manual 6 Error Information What Are Error Messages 53 General Error Messages 54 Timeout 54 Shutdown 54 Remote Timeout 55 Lost CAN Partner 56 Leak Sensor Short 56 Leak Sensor Open 57 Compensation Sensor Open 57 Compensation Sensor Short 58 Fan Failed 59 Leak 59 Pump Error Messages 60 Pressure of quaternary pump above upper limit 60 Pressure below lower limit 60 Target pressure not reached for quaternary pump degasser 61 Solvent counter exceeded limit 61 Waste counter limit exceeded 62 Flow rate limit exceeded 62 Quaternary pump shutdown during analysis 63 Reading the pump encoder tag failed 63 Writing the pump encoder tag failed 64 Pump drive blocked or encoder failed 64 Drive current too low 65 Drive current too high 65 Drive timeout 66 Overcurrent of pump drive 66 Deliver underrun 67 ae Agilent Technologies 51 6 Error Information Defect connection between main board and pump drive encoder 67 Pump drive encoder defect 68 Multi Purpose Valve failed 68 Reading of multi purpose valve tag failed 69 Pump drive encoder rollover 69 Drive position limit 70 Insufficient power of drive encoder LED 70 Drive encoder error 70 Writing the multi purpose valve tag failed 71 Unknown multi purpose valve ty
71. essibility compensation Recommended pH range Gradient formation Delay volume Composition range Composition precision Composition accuracy Number of solvent Dual pistons in series pump with proprietary servo controlled variable stroke design and smooth motion control for active damping 300 pL step size 0 001 5 mL min in 0 001 mL min increments executed in 300 pL step increments lt 0 07 RSD or 0 01 min SD whatever is greater 1 or 10 pL min whatever is greater up to 130 MPa 1300 bar at 0 2 mL min ramping down to 80 MPa 800 bar at 5 mL min lt 1 amplitude or lt 0 5 MPa 5 bar whatever is greater Automatic 1 0 12 5 solvents with pH lt 2 3 should not contain acid which attack stainless steel Low pressure quaternary mixing As low as 350 pL Settable range 0 100 Recommended range 1 99 or 5 pL min lt 0 15 RSD or 0 02 min SD whatever is greater 0 4 absolute 1 99 B 4 out of maximum 26 solvents Agilent 1290 Infinity II Flexible Pump User Manual Table 2 Site Requirements and Specifications 2 Agilent 1290 Infinity II Flexible Pump G7104A Performance Specifications Feature Specification Solvent selection valve Degassing unit Materials in contact with solvent Automatic Purge Valve Active Seal wash Intelligent System Emulation Technology ISET Communications Safety and maintenance GLP features Hou
72. et that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Agilent 1290 Infinity II Flexible Pump User Manual 25 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 1 on page 27 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation can damage the system electronics gt Do not store ship or use your module under conditions where tempe
73. feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Agilent 1290 Infinity II Flexible Pump User Manual 187 10 Hardware Information Instrument Layout The industrial design of the mo
74. g a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 5 Nm and fix the 4 screws at the rear of the alignment tool Tighten screws crosswise KN This procedure will align pump head parts to their correct positions and close the pump head tightly 10 Open the 3 screws which have closed the pump head alignment tool and take out the aligned pump head In case the pump head sticks inside the alignment tool you can use the handle and insert it to the rear of the tool for pushing out the pump head Heat exchanger capillary ool han GO i Agilent 1290 Infinity I Flexible Pump User Manual 127 7 Maintenance CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 11 For the primary pump head install the inlet valve and outlet valve using the torque wrench which is included to the 1290 Infinity Service Kit p n 5067 4699 Set 10 Nm for the inlet valve and 10 Nm for the outlet valve CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 12 For the secondary pump head install the outlet filter adapter using the torque wrench 14 mm hex bit which is included to t
75. g the BootP mode instead of Using Stored or Using Default modes Agilent 1290 Infinity II Flexible Pump User Manual LAN Configuration 11 Installation of BootP Service Before installing and configuring the Agilent BootP Service be sure to have the IP addresses of the computer and instruments on hand 1 2 3 Log on as Administrator or other user with Administrator privileges Close all Windows programs Insert the Agilent ChemStation software DVD into the drive If the setup program starts automatically click Cancel to stop it 4 Open Windows Explorer 5 Go to the BootP directory on the Agilent ChemStation DVD and double click BootPPackage msi If necessary click the Agilent BootP Service icon in the task bar The Welcome screen of the Agilent BootP Service Setup Wizard appears Click Next The End User License Agreement screen appears Read the terms indicate acceptance then click Next The Destination Folder selection screen appears Install BootP to the default folder or click Browse to choose another location Click Next The default location for installation is C Program Files Agilent BootPService 10 Click Install to begin installation Agilent 1290 Infinity II Flexible Pump User Manual 203 11 LAN Configuration 11 Files load when finished the BootP Settings screen appears BootP Settings a BootP Tab File Create Tab File Edit BootP Addresses Logging BootP Log Fi
76. gh pressure outlet filter 4 Replace the filter frit 5 Mount the high pressure outlet filter using a torque 6 Reinstall the high pressure outlet filter to the filter holder wrench set to 16 Nm 7 Mount the capillary connection to the pressure sensor 1 and to the Multipurpose valve 2 Agilent 1290 Infinity I Flexible Pump User Manual 137 7 Maintenance heplace Parts of the Inline Replace Parts of the Inline Filter Tools required p n 8710 0510 Parts required p n 5067 5407 5067 4748 5023 0271 Description Wrench open 1 4 5 16 inch Description In Line Filter Assembly for 1290 Infinity Quaternary Pump Capillary ST 0 17 mm x 90 mm Multi Purpose Valve to inline filter Frit 0 3 ym for inline filter 5 pk CAUTION Stuck Capillary in Multi Purpose Valve Shortcutting the inline filter by directly connecting its right capillary to valve port 5 can damage the Multi Purpose Valve The size position of this capillary in its fitting is incompatible so it may get stuck irreversibly to the valve gt Do not shortcut the filter by directly connecting its right capillary to valve port 5 in case the inline filter cannot or shall not be used yp The inline filter can be cleaned using the back flush function in the user interface of your Agilent instrument control software 1 Remove the capillaries from the Multi Purpose Valve to 2 Remove the inline filter from the cla
77. gnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields Indicates a pinching or crushing hazard A A Indicates a piercing or cutting hazard EA es alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions Agilent 1290 Infinity II Flexible Pump User Manual 225 12 Appendix Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose of in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for
78. h solvents 37 Select channels for Multi Channel Gradient Valve MCGV 37 This chapter explains the operational parameters of the Agilent 1290 Infinity II Flexible Pump Ee Agilent Technologies 31 3 Using the Pump Magnets Magnets 32 Agilent 1290 Infinity II Flexible Pump User Manual Using the Pump 3 Turn on off Turn on off Power switch 1 On 2 Off Agilent 1290 Infinity I Flexible Pump User Manual 33 3 Using the Pump Status Indicators The module status indicator indicates one of six possible module conditions Status indicators 1 Idle 2 Run mode 3 Not ready Waiting for a specific pre run condition to be reached or completed 4 Error mode interrupts the analysis and requires attention for example a leak or defective internal components 5 Resident mode blinking for example during update of main firmware 6 Bootloader mode fast blinking Try to re boot the module or try a cold start Then try a firmware update 34 Agilent 1290 Infinity II Flexible Pump User Manual Using the Pump 3 Best Practices Daily Weekly tasks Daily tasks e Replace mobile phase based on water buffer e Replace organic mobile phase latest every 2nd day e Check seal wash solvent e Run conditioning with composition of your application Weekly tasks e Change seal wash solvent 10 90 isopropanol water and bottle e Flush all channels with water to r
79. he 1290 Infinity Service Kit p n 5067 4699 set to 16 Nm 128 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance Pump Head Procedures 7 13 Remove the alignment piston 14 Insert the pump piston Piston Agilent 1290 Infinity I Flexible Pump User Manual 129 7 Maintenance Reassemble the Pump Head Assembly 1 Insert both pump heads to the link plate and make sure CAUTION that the clips snap in that fix the pump heads CAUTION aD Damage to the pump head Link plate a T Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 2 Insert the heat exchanger capillary into the outlet of the primary pump head Using a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 3 Nm and close the hex screw at the top of the outlet 2 mD Clips Observe the correct orientation of the primary and the secondary pump head This is important for correct fixation of the heat exchanger and the capillaries as described in the following steps 130 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Pump Head Procedures Install the Pump Head Assembly 1 Bring the pump drive to the maintenance position using the Lab Advisor user interface Go to Tools gt Remove Install Pump Head and follow instructions give
80. hub the LAN interface will be automatically set to operate at 10 Mbps half duplex If the module is not able to connect to the network through auto negotiation you can manually set the link operating mode using link configuration switches on the module Table 15 Link Configuration Switches SW3 SW4 SW5 Link Configuration OFF speed and duplex mode determined by auto negotiation ON OFF OFF manually set to 10 Mbps half duplex ON OFF ON manually set to 10 Mbps full duplex ON ON OFF manually set to 100 Mbps half duplex ON ON ON manually set to 100 Mbps full duplex 200 Agilent 1290 Infinity II Flexible Pump User Manual LAN Configuration 11 Automatic configuration with Bootp All examples shown in this chapter will not work in your environment You need your own IP Subnet Mask and Gateway addresses Assure that the detector configuration switch is set properly The setting should be either BootP or BootP amp Store see Table 11 on page 193 Assure that the detector connected to the network is powered off If the Agilent BootP Service program is not already installed on your PC then install it from your Agilent ChemStation DVD located in folder BootP About Agilent BootP Service The Agilent BootP Service is used to assign the LAN Interface with an IP address The Agilent BootP Service is provided on the ChemStation DVD The Agilent BootP Service is installed on a server or PC on the LAN to provide c
81. ient separations this volume causes a delay between the mixture changing in the pump and that change reaching the column The delay depends on the flow rate and the delay volume of the system In effect this means that in every HPLC system there is an additional isocratic segment in the gradient profile at the start of every run Usually the gradient profile is reported in terms of the mixture settings at the pump and the delay volume is not quoted even though this will have an effect on the chromatography This effect becomes more significant at low flow rates and small column volumes and can have a large impact on the transferability of gradient methods It is important therefore for fast gradient separations to have small delay volumes especially with narrow bore columns e g 2 1 mm i d as often used with mass spectrometric detection The delay volume in a system includes the volume in the pump from the point of mixing connections between pump and autosampler volume of the flow path through the autosampler and connections between autosampler and column For the 1290 Infinity II Flexible Pump all pump parts downstream the MCGV contribute to the delay volume i e inlet weaver pump heads capillary connections filters and the optional Jet Weaver 40 Agilent 1290 Infinity II Flexible Pump User Manual How to Optimize the Performance of YourModule 4 How to Configure the Optimum Delay Volume The design of the 1290 Infinity Quatern
82. iew of the Flexible Pump 178 Agilent 1290 Infinity I Flexible Pump User Manual Interfaces Hardware Information 10 The Agilent 1200 Infinity Series II modules provide the following interfaces Table4 Agilent 1200 Infinity II Series Interfaces Module CAN USB LAN RS 232 Analog APG Special on board A ERI E Pumps G7104A Flexible Pump 2 No Yes Yes 1 G7120A High Speed Pump 2 No Yes Yes 1 A Samplers G7129A B ALS 2 Yes Yes No No E G7167A B Multisampler 2 Yes Yes No No E Detectors G7114A B VWD 2 Yes Yes No 1 E G7117A B DAD 2 Yes Yes No 1 E G7115A B DAD 2 Yes Yes No 1 E Others G7116B MCT 2 No No No No No Requires a HOST module via CAN The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN Agilent 1290 Infinity II Flexible Pump User Manual 179 10 Hardware Information e CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer e USB Universal Series Bus as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369
83. ilent 1290 Infinity II Flexible Pump User Manual Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation 55 6 Error Information Lost CAN Partner Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sen
84. ill be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface Agilent 1290 Infinity II Flexible Pump User Manual 53 6 Error Information General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold
85. ilter adapter on the secondary pump head to the pressure sensor 106 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Pump Head Procedures 6 Use the mounting tool for removing tubing connections between the degassing unit and the MCGV 7 Open the black plastic screw of the inlet valve at the bottom of the left primary pump head 1 and remove the inlet weaver by first pushing it downwards 2 and then pulling it out to the left 3 8 Open the four screws holding the pump heads NOTE Open the screws step by step not screw by screw 9 Remove the complete pump head assembly by holding both heads and pulling it to the front Disassemble the Pump Head Assembly on page 108 10 Proceed to install see Install the Pump Head Assembly on page 131 or disassemble the pump head see Agilent 1290 Infinity I Flexible Pump User Manual 107 7 Maintenance Disassemble the Pump Head Assembly When If parts inside the pump head need to be replaced Tools required p n Description G4220 20012 Torque wrench 2 25 Nm G4220 20013 4 mm hex bit G4220 20014 2 5 mm Hex Bit G4220 20015 Adapter in square to hex 8710 0510 Wrench open 1 4 5 16 inch Preparations Remove the pump head CAUTION Damaged pump head Disassembling or reassembling the pump head with tools other than the ones recommended can damage pump heads and significantly reduce their life time gt Fol
86. in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Agilent 1290 Infinity II Flexible Pump User Manual 175 10 Hardware Information Main FW update Resident System Main System Resident FW Update Figure 19 Firmware Update Mechanism Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 176 Agilent 1290 Infinity II Flexible Pump User Manual Hardware Information 10 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e The REMOTE connector may be used in combin
87. in until the release buttons click into their final position 1 Press the release buttons and pull the front door out Agilent 1290 Infinity I Flexible Pump User Manual 87 7 Maintenance Replace the Pressure Sensor Replace the Pressure Sensor When No or invalid pressure signal Tools required p n Description 8710 2412 Hex key 2 5 mm 15 cm long straight handle 8710 0510 Wrench open 1 4 5 16 inch Screwdriver Parts required p n Description G7104 60001 Pressure sensor 1300 bar Preparations Turn off pump flow switch off pump NOTE This procedure describes how to replace the pressure sensor In case the cable to the sensor shall be replaced as well please contact your Agilent service representative NOTE Working on connections to the pressure sensor may slightly change the displayed pressure In case of a pressure offset at ambient pressure a pressure offset calibration may be run 1 Remove capillary connections between the pressure 2 Remove the screws that fix the pressure sensor to the sensor and the High Pressure Filter and between the chassis pressure sensor and the outlet filter of the secondary pump head respectively 88 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Replace the Pressure Sensor 3 Carefully pull out the pressure sensor for about 2 cm Then unscrew the cable from the pressure sensor 4 Connect the new pressure sens
88. inity II Flexible Pump User Manual 155 8 Parts and Materials Accessory Kit G7104 68705 p n 0100 1816 0890 2207 5023 0271 5043 1013 5063 6527 5067 4670 5067 5443 5181 1519 5500 1155 5067 6129 9301 6476 G1311 90107 9301 1337 5500 1156 5500 1169 156 Description Fitting Waste Tube to Purge Valve Tubing Sleeving Flex Frit 0 3 pm for inline filter 5 pk Tubing Clip Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste SST cap 0 17 mm ID 600 mm pre swaged Inlet tubing CAN cable Agilent module to module 1 m Tube Connector 90 degree ID 6 4 Capillary ST 0 17 x 300 mm S SX Syringe with luerlock 5 mL Polypropylene Algae note Syringe adapter T Tube Connector ID6 4 Y Tube Connector ID6 4 Agilent 1290 Infinity II Flexible Pump User Manual Tool Kit p n 8720 0025 8710 1924 8710 2409 8710 0510 5023 2500 8710 1534 5043 1361 5023 2499 8710 2394 8710 0899 5023 2501 5023 2504 5023 2503 5023 2502 9301 0411 9301 1337 0100 1710 0100 1681 01018 23702 5067 6127 Parts and Materials 8 Description Wrench 1 2 inch amp 9 16 inch Wrench open 14 mm Wrench open end 5 16 3 8 inch Wrench open 1 4 5 16 inch Wrench Wrench 4 mm both ends open end Hex Key Set Driver Hex Key Set Hex key 9 64 inch 15 cm long T handle Pozidriv screwdriver Screwdriver Torx T10 Hex driver SW 4 slitted Hex driver SW 5 slitted Hex driver 1 4 inch slitted Syringe Plastic Syringe adapter Mounting
89. le ESS ad Sa REG Default Settings Subnet mask GO 0 0 0 Gateway GO 0 0 0 Cancel Help Figure 32 BootP Settings screen 12 In the Default Settings part of the screen if known you can enter the subnet mask and gateway Defaults can be used The default subnet mask is 255 255 255 0 The default gateway is 192 168 254 11 13 On the BootP Settings screen click OK The Agilent BootP Service Setup screen indicates completion 14 Click Finish to exit the Agilent BootP Service Setup screen 15 Remove the DVD from the drive This completes installation 16 Start BootP Service in the Windows services On the Windows desktop click right on Computer icon select Manage gt Services and Applications gt Services Select the Agilent BootP Service and click Start 204 Agilent 1290 Infinity II Flexible Pump User Manual LAN Configuration 11 Two Methods to Determine the MAC Address Enabling logging to discover the MAC address using BootP If you want to see the MAC address select the Do you want to log BootP requests check box 1 Open BootP Settings from Start gt All Programs gt Agilent BootP Service gt EditBootPSettings 2 In BootP Settings check Do you want to log BootP requests to enable logging Logging MV Do you want to log bootP requests BootP Log File EOS Figure 33 Enable BootP logging The log file is located in C Documents and Settings All Users Application Data Agilent
90. low all instructions step by step gt Use recommended tools like the pump head alignment tool and a torque wrench 108 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Pump Head Procedures 1 Place the pump head on a special holding tool 2 Open the 2 5 mm hex or Tx 10 screw at the top of the primary pump head which fixes the connection capillary of the heat exchanger 1 Then lift up the capillary and remove it from the primary pump head 2 A gold seal between outlet valve and heat exchanger capillary is used for a tight connection 3 Remove both pump heads from the link plate by pushing the clips at the rear of the plate Link plate Clips 4 Remove the outlet valve at the top of the pump head and the inlet valve at the bottom of the primary pump head Outlet valve Inlet valve Agilent 1290 Infinity I Flexible Pump User Manual 109 7 Maintenance Pump Head Procedures 5 Remove the outlet filter at the top of the secondary pump head 110 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Disassemble the Primary Pump Head INET Damage of pump piston The pump piston is made of Zr07 based ceramic which is a very hard and resistant material but it is sensitive to shearing forces from the side gt Do not try to remove the pump piston from the rear gt Do not use the piston for removing pump seals 1 For disas
91. low rate rate e Increase pressure limit Pressure below lower limit Error ID 29176 The pressure has dropped below the lower limit e Parameter None Probable cause Suggested actions 1 Leak Check for leaks 2 Bottle empty Check bottle filling 3 Wrong solvent viscosity Check solvent 4 Inappropriate setting Check flow rate and lower pressure limit 5 Column degradation Replace column 60 Agilent 1290 Infinity II Flexible Pump User Manual Error Information 6 Target pressure not reached for quaternary pump degasser Error ID 29221 The target pressure of the quaternary pump degasser has not been reached within the expected time Parameter Pressure in mbar Probable cause Suggested actions 1 Condensation in degasser chamber due to Equilibrate and restart module temperature fluctuation 2 Degasser is defect Please contact your Agilent service representative Solvent counter exceeded limit Error ID 29146 The counter for the solvent volume has exceeded the limit which has been set in the user interface Probable cause Suggested actions 1 No solvent present Refill solvent bottle 2 Inappropriate setting Check solvent counter setting in user interface Agilent 1290 Infinity II Flexible Pump User Manual 61 6 Error Information Waste counter limit exceeded Error ID 29147 The counter for the waste volume has exceeded the limit which has been set in the user interface e Parameter None
92. lve MCGV and or degasser gt Never apply pressure to the MCGV or degasser gt Directly connect the syringe to the inlet weaver 1 Remove tubing connections channels A B C and D to the MCGV such that you can access the inlet weaver 2 Open the fitting at the center of the multi channel gradient valve MCGV Do not open the screw marked with the red cross Remove the inlet weaver from the MCGV Fitting 102 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance Release a Stuck Inlet Valve 7 3 Slightly open the black plastic screw at the bottom of the inlet valve and rotate the inlet weaver to the front Then retighten the screw 4 Disconnect the capillary from the pressure sensor inlet and route the capillary to a small beaker 5 Fill the syringe with a suitable wash solvent NOTE For salt deposits warm water is a good choice For organic deposits use ethanol or acetone 7 Push the syringe for flushing the inlet valve and pump head 8 Restore original connections Flush the system for several minutes Agilent 1290 Infinity I Flexible Pump User Manual 103 7 Maintenance Pump Head Procedures CAUTION Limitation of life time The pump head assembly is an exchange part which cannot be reassembled with standard tools Disassembling the pump head will strongly limit its life time gt Do not disassemble the pump head as
93. mation 220 Safety Standards 220 General 220 Before Applying Power 221 Ground the Instrument 221 Do Not Operate in an Explosive Atmosphere 222 Do Not Remove the Instrument Cover 222 Do Not Modify the Instrument 222 In Case of Damage 222 Solvents 223 Safety Symbols 224 Waste Electrical and Electronic Equipment Directive 226 Radio Interference 227 Sound Emission 228 Agilent Technologies on Internet 229 This chapter provides additional information on safety legal and web he Agilent Technologies 219 12 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards General Do not use this product in any manner not
94. mation of the multi purpose valve is invalid e Parameter None Probable cause Suggested actions 1 Wrong valve head installed Check or replace multi purpose valve head 2 Valve head has invalid RFID tag content Check or replace multi purpose valve head Pump drive encoder error Error ID 29211 The pump drive encoder has generated no signal Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Agilent 1290 Infinity II Flexible Pump User Manual 71 6 72 Error Information Pump drive error Error ID 29212 29213 The pump drive failed during calibration e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Maximum stroke is too short Error ID 29203 The maximum stroke is too short During initialization the pump defines the operation position of the pump drives and therefore the pistons First the pump drive moves backwards to find a mechanical stop within the ball screw Afterwards pistons move forwards for finding the maximum available stroke volume These values are expected within a pre defined range Maximum stroke too short means that the outer drive position is too close This can be caused by a drive initialization without pump head or if the pump head has not been installed properly sc
95. mber or degasser tubing 2 Defect vacuum pump 3 Degasser chamber empty or connected to air Error ID 29223 Error Information Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Block unused degasser channels Seal wash pump was missing when tried to turn on The seal wash pump has not been detected while being configured or detected before Probable cause 1 Defect cable connection to seal wash pump 2 Defect seal wash pump motor 3 Defective main board Agilent 1290 Infinity II Flexible Pump User Manual Suggested actions Check cable connection Please contact your Agilent service representative Please contact your Agilent service representative 77 6 78 6 Error Information Valve hardware overcurrent MCGV Error ID 29227 Power consumption too high for one of the MCGV valves Probable cause 1 Cable defect 2 Valve defect 3 Defective main board Suggested actions Replace MCGV Replace MCGV Please contact your Agilent service representative Agilent 1290 Infinity II Flexible Pump User Manual Agilent 1290 Infinity Il Flexible Pump User Manual 7 Maintenance Introduction to Maintenance 81 Warnings and Cautions 82 Overview of Maintenance 84 Cleaning the Module 85 Install Fittings and Capillaries 86 Remove and Install Doors 87 Replace the Pressure Sensor 88 Replace the Inlet Wea
96. more information 226 Agilent 1290 Infinity II Flexible Pump User Manual Appendix 12 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent 1290 Infinity II Flexible Pump User Manual 227 12 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 228 Agilent 1290 Infinity II Flexible Pump User Manual Appendix 12 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Agilent 1290 Infinity II Flexible Pump User Manual 229 Index Index 8 bit configuration switch on board LAN 184 A Agilent Lab Advisor software 49 Agilent Lab Advisor 49 Agilent oninternet 229 User Interface Softwa
97. mp User Manual LAN Configuration 11 Using Stored When initialization mode Using Stored is selected the parameters are taken from the non volatile memory of the module The TCP IP connection will be established using these parameters The parameters were configured previously by one of the described methods Non Volatile RAM Active Parameter Stored Parameter Figure 28 Using Stored Principle Using Default When Using Default is selected the factory default parameters are taken instead These parameters enable a TCP IP connection to the LAN interface without further configuration see Table 12 on page 195 Default Active Parameter Parameter Figure 29 Using Default Principle Using the default address in your local area network may result in network problems Take care and change it to a valid address immediately Table 12 Using Default Parameters IP address 192 168 254 11 Subnet Mask 255 255 255 0 Default Gateway not specified Agilent 1290 Infinity II Flexible Pump User Manual 195 11 LAN Configuration 196 Since the default IP address is a so called local address it will not be routed by any network device Thus the PC and the module must reside in the same subnet The user may open a Telnet session using the default IP address and change the parameters stored in the non volatile memory of the module He may then close the session select the initialization mode Using Stored power
98. mp attached to the the inline filter Inline filter 138 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Replace Parts of the Inline Filter 3 Use two 5 16 wrenches for opening the inline filter 4 Replace the filter frit and reassemble the inline filter Filter frit 5 Put the inline filter to the clamp and install its capillaries The integrated capillary is connected to port 5 of the Multi Purpose Valve The removable capillary is connected to port 8 Agilent 1290 Infinity I Flexible Pump User Manual 139 7 Maintenance Replace the Module Firmware When Tools required OR Parts required Preparations 140 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version Description Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Downlo
99. n Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces The module includes a DC Out 24 VDC power line that is intended to be used with certain modules that operate as CAN slaves for example external valves The line has a limited output of 1 7 A and is self resetting Agilent 1290 Infinity II Flexible Pump User Manual 183 10 Hardware Information Setting the 8 bit Configuration Switch The 8 bit configuration switch is located at the rear of the module Switch settings provide configuration parameters for LAN serial communication protocol and instrument specific initialization procedures All modules with on board LAN e Default is ALL switches DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 e For specific LAN modes switches 3 8 must be set as required e For boot test modes switches 1 2 must be UP plus required mode For normal operation use the default best settings SX Agilent Tecdnsiogies YVUUYUUEUUYUY 184 Figure 22 Location of Configuration Switch example shows a G4212A DAD Agilent 1290 Infinity II Flexible Pump User Manual Hardware Information 10 To perform any LAN configuration SW1 and SW2 must be set to OFF For details on the LAN settings configurati
100. n on the screen Both pump drives must be retracted Wrong Correct Agilent 1290 Infinity I Flexible Pump User Manual gt For handling the torque wrench setting and applying CAUTION Damage to the pump head Using a wrong torque will damage the pump head the right torque consult the manual of your torque wrench 2 Install the new pump head assembly by tightening the screws step by step Apply 5 Nm using a torque hex key which is included to the 1290 Infinity Service Kit p n 5067 4699 131 7 Maintenance Pump Head Procedures 3 5 Insert the new inlet weaver to the inlet valve 1 2 Fix the weaver with the plastic screw to the inlet valve 3 Connection to inlet weaver Connect the capillary from the outlet filter adapter on the secondary pump head to the pressure sensor 4 Reconnect solvent tubes for channels A D from the MCGV to the degasser outlets 6 Connect the seal wash tubes Next Steps 7 Close the doors 8 Perform a Pump Leak Rate Test and a System Pressure Test 132 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Replace the Multi Purpose Valve Replace the Multi Purpose Valve Tools required p n Description 5023 0240 Hex driver 4 slitted Parts required p n Description 0100 1259 Blank nut plastic 01080 83202 Blank nut stainless steel 5067 4237 Multi Purpose Valve Head Preparations Remove all c
101. nd Secondary Pump Head When Tools required Parts required CAUTION This procedure describes how to assemble the secondary pump head using the pump head alignment tool Assembling the primary pump head can be done accordingly The secondary pump head has the heat exchanger capillary which must fit into the openings of the alignment tool whereas the primary pump head does not have a heat exchanger Before installing the pump head p n Description Pump head alignment tool G4220 20012 Torque wrench 2 25 Nm G4220 20013 4 mm hex bit G4220 20041 Bit Torx 10x25 mm G4220 20015 Adapter in square to hex 01018 23702 Insert tool p n Description 0905 1719 Metering seal 100 pL 0905 1718 Wash Seal PE 5062 2484 Gasket seal wash pack of 6 See chapter Parts for details Damage of the pump piston The pump piston is very sensitive to shearing forces from the side gt Use the alignment piston of the pump head alignment tool for the alignment procedure described below CAUTION Wrong orientation of pins on support ring Assembling the pump head without paying attention to the correct orientation of the pins on the support ring can lead to leaks or damage of the piston and pump head gt Observe pins on the support ring which help assembling the parts of the pump head in the correct orientation Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 CAUTION Damage of the pump head assembly When i
102. nfiguration selection 200 lost CAN partner 56 M MAC address determine 206 MAC address 190 maintenance 3 3 3 3 232 feedback 187 introduction 81 replacing firmware 140 anual configuration of LAN 211 maximum stroke too short 72 MCGV replace 100 message remote timeout 55 module firmware replace 140 ulti channel gradient valve replace 100 ulti purpose valve failed 68 ulti purpose valve parts 154 replace parts 135 replace 133 non operating altitude 27 non operating temperature 27 0 operating Altitude 27 operating principle 12 operating temperature 27 optimization achieving higher resolution 43 outlet valve replace 93 overcurrent of pump drive 66 overview cable 162 P parts main assemblies 144 multi purpose valve 154 primary pump head 150 secondary pump head 152 PC and User Interface Software Setup 216 performance specifications 28 28 performance Optimization 39 physical specifications 27 power consideration 24 power consumption 27 power cords 25 pressure below lower limit 60 pressure exceeded upper pressure limit 60 pressure sensor replace 88 primary pump head assemble 122 disassemble 111 parts 150 product description 10 ump drive blocked or encoder failed 64 ump drive encoder defect 68 ump drive encoder error 71 ump drive encoder rollover 69 ump drive error 72 ump drive stop not found 73 ump error messages 60 T 7T uG vv O F G
103. nsert the alignment piston of the pump head alignment CAUTION a CAUTION Damage to the pump head uf gt Using the alignment tool is mandatory Alignment piston j gt Not using will break the pump head 6 Insert the pump head to the pump head alignment tool lt i which is included to the 1290 Infinity Service Kit p n 5067 4699 There are openings for the seal wash support ring and heat exchanger of the secondary pump head N A Observe the correct orientation of all parts j Pump head alignment tool rear a lt QU Pump head alignment tool front Agilent 1290 Infinity I Flexible Pump User Manual 125 7 Maintenance 7 Close the tool by closing the 3 screws at the connection ring Heat exchanger capillary CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 8 Using a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 5 Nm and fix the central alignment screw entra alignment screw 126 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 9 Usin
104. nstalling the pump head assembly the pump drives need to be in maintenance position where they are retracted Using the pump drive in default position will damage the pump head assembly gt Bring the pump drive to the maintenance position Vivi Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 1 Use the soft plastic side of the insert tool for inserting the 2 Insert the support ring and pump head ferrules into the piston seal to the pump chamber housing piston housing Observe the pins on the support ring which help you assembling the pump head correctly Insert tool Pump seal eG SS Pins second pinnotshown Pump chamber housing Pump head ferrules Support ring Agilent 1290 Infinity I Flexible Pump User Manual 123 7 Maintenance Pump Head Procedures 3 Assemble the pump head by putting the pump chamber housing on top of the support ring Observe correct orientation of the pin Pump chamber housing Pump head ferrules Support ring Piston housing NOTE Do NOT install the inlet and outlet valves and the outlet filter at this stage 4 Loosely close the 4 screws at the rear of the pump head The screws will be fixed tightly later 124 Agilent 1290 Infinity I Flexible Pump User Manual Maintenance 7 5 I
105. nt filters inside solvent bottles for avoiding leakages 1 Remove the cap from the outlet valve 2 Open the 2 5 mm hex screw at the top of the primary pump head which fixes the connection capillary of the heat exchanger 1 and remove it 2 Then lift up the capillary and remove it from the primary pump head 3 Agilent 1290 Infinity I Flexible Pump User Manual 93 7 Maintenance Replace the Outlet Valve 3 A gold seal between outlet valve and heat exchanger 4 Unscrew the outlet valve with a 14 mm wrench 1 and capillary is used for a tight connection The seal can be remove it 2 replaced separately as needed 5 Insert the new outlet valve and tighten it using a torque 6 Insert the heat exchanger capillary into the outlet of the wrench with a 14 mm bit set to 10 Nm outlet valve 1 Using a torque wrench with a 2 5 mm hex bit set 3 Nm and close the hex screw at the top of the outlet 2 7 Replace the cap of the Outlet Valve 8 Purge the system to remove air 94 Agilent 1290 Infinity II Flexible Pump User Manual Remove the Jet Weaver Maintenance 7 Remove the Jet Weaver Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 0899 Pozidriv screwdriver Parts required p n Description 2 0100 1259 Plastic fittings Preparations Select Do not use mixer in ChemStation Switch off the pump at the main power switch 1 Remove capillary connections
106. olution equation 1 a 1 k Rs VN 2 i 4 a k where R resolution e N plate count measure of column efficiency e o selectivity between two peaks e ko retention factor of second peak formerly called capacity factor The term that has the most significant effect on resolution is the selectivity a and practically varying this term involves changing the type of stationary phase C18 C8 phenyl nitrile etc the mobile phase and temperature to maximize the selectivity differences between the solutes to be separated This is a substantial piece of work which is best done with an automated method development system which allows a wide range of conditions on different columns and mobile phases to be assessed in an ordered scouting protocol This section considers how to get higher resolution with any chosen stationary and mobile phases If an automated method development system was used in the decision on phases it is likely that short columns were used for fast analysis in each step of the scouting The resolution equation shows that the next most significant term is the plate count or efficiency N and this can be optimized in a number of Agilent 1290 Infinity II Flexible Pump User Manual 43 4 How to Optimize the Performance of Your Module ways N is inversely proportional to the particle size and directly proportional to the length of a column and so smaller particle size and a longer column will give
107. om instruments guides the solvent to a suitable waste container Agilent 1290 Infinity II Flexible Pump User Manual Introduction 1 Waste Concept Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports 5043 1221 for optimal and safe waste disposal If you decide to use your own waste solution make sure that the tubes don t immerse in the liquid Agilent 1290 Infinity I Flexible Pump User Manual 21 1 22 Introduction Leak and Waste Handling Agilent 1290 Infinity II Flexible Pump User Manual Agilent 1290 Infinity Il Flexible Pump User Manual e00 ee 2 r Site Requirements and Specifications Site Requirements 24 Physical Specifications 27 Performance Specifications 28 This chapter provides information on environmental requirements physical and performance specifications ee Agilent Technologies 23 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 1 on page 27 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Hazard of electrical shock or damage of your instrumentation can result if the devices are connect
108. on refer to chapter LAN Configuration Table 7 8 bit Configuration Switch with on board LAN Mode Function sW 1 SW 2 SW 3 SW 4 SW 5 SW6 SW7 SW 8 LAN 0 0 Link Configuration Init Mode Selection Auto negotiation 0 x x Xx xX X 10 MBit half duplex 1 0 0 x xX X 10 MBit full duplex 1 0 1 x xX X 100 MBit half duplex 1 1 0 x X xX 100 MBit full duplex 1 1 1 x X x Bootp x Xx Xx 0 0 0 Bootp amp Store Xx Xx x 0 0 1 Using Stored x x Xx 0 1 0 DHCP Xx Xx xX 1 0 0 Using Default x X x 0 1 1 TEST 1 1 System NVRAM Boot Resident System 1 x Revert to Default Data Coldstart x Xx x 1 Legend 0 switch down 1 switch up x any position When selecting the mode TEST the LAN settings are Auto Negotiation amp Using Stored For explanation of Boot Resident System and Revert to Default Data Coldstart refer to Special Settings on page 186 Agilent 1290 Infinity II Flexible Pump User Manual 185 10 Hardware Information Special Settings The special settings are required for specific actions normally in a service case The tables include both settings for modules with on board LAN and without on board LAN They are identified as LAN and no LAN Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode
109. opening in the front panel 1 and push it down 2 4 Fix the Jet Weaver with a screw 5 Mount both capillary connections to the Jet Weaver observing the correct orientation 6 Connect the inlet capillary of the Jet Weaver to port 2 of the Multi Purpose Valve Connect the outlet capillary to port 1 7 Configure the Jet Weaver as mixer in the user interface Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Replace the Seal Wash Pump Replace the Seal Wash Pump When In case of wear of the seal wash pump Parts required p n Description 5065 4445 Peristaltic pump with Pharmed tubing 5065 9978 Tubing 1 mm i d 3 mm o d silicone 5m Preparations Remove the flow connections from and to the seal wash pump 1 For removing the seal wash pump press the clips 1 and 2 Insert the pump clips to the holes in the module housing pull the pump to the front 2 3 Fix the seal wash tubings to the peristaltic pump inlet 1 and the from the peristaltic pump outlet to the primary pump head inlet 2 Agilent 1290 Infinity I Flexible Pump User Manual 99 7 Maintenance Replace the Multi Channel Gradient Valve MCGV Tools required p n Description 0100 1710 Mounting Tool for Tubing Connections 8710 0899 Pozidriv screwdriver Parts required p n Description G1311 67701 Multi channel gradient valve MCGV Preparations Switch off pump at the
110. or to the pressure sensor connector amp 5 Fix the pressure sensor to the instrument chassis Agilent 1290 Infinity I Flexible Pump User Manual 6 Connect the capillary from the pump head outlet to the pressure sensor inlet 1 Connect the capillary from the high pressure filter to the pressure sensor outlet 2 Two arrow signs on the pressure sensor indicate the flow direction 7 Maintenance Replace the Inlet Weaver Replace the Inlet Weaver Parts required p n Description G4204 81090 1290 Infinity Quaternary Pump Inlet Weaver Assembly Preparations Switch off pump at the main power switch Open the doors Use an optional solvent shutoff valve or lift up solvent filters inside solvent bottles for avoiding leakages For easy access to the inlet weaver assembly remove tubing connections between MCGV and degasser 1 Open the screw at the bottom of the inlet valve 2 Open the fitting at the center of the multi channel gradient valve MCGV Do not open the screw marked with the black cross Remove the inlet weaver from the MCGV 90 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Replace the Inlet Weaver 3 Pull the Inlet Weaver out of the Inlet Valve 4 Insert the new inlet weaver to the inlet valve Fix the weaver with the plastic screw 5 Fix the fitting of the new inlet weaver to the MCGV 6 Reconnect tubings between MCGV and degasser
111. out Wait for Pressure Error ID 29185 A target condition pressure has been sent to the instrument which should have been reached within an expected time frame but didn t Either the limit time frame or the current value of the variable has been modified later directly or indirectly Probable cause Suggested actions 1 Incorrect parameters have been senttothe Verify control software macros manual instrument by the control software or commands manual changes 2 Leak Run system pressure test for identifying and localizing the leak Tighten leak Agilent 1290 Infinity II Flexible Pump User Manual 75 6 76 Error Information Drive Encoder failed Error ID 29210 Drive encoder failed during pump drive calibration Probable cause Suggested actions 1 Internal error Contact Agilent support Drive phases differ too much in electric resistance Error ID 29216 Pump drive calibration has failed due to a strong difference electric resistances for different motor phases Probable cause Suggested actions 1 Pump drive cable defect Please contact your Agilent service representative 2 Pump drive defect Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Agilent 1290 Infinity II Flexible Pump User Manual Degasser s pressure limit violation Error ID 29220 Pressure too far above the limit Probable cause 1 Leak in degasser cha
112. pe 71 Pump drive encoder error 71 Pump drive error 72 Maximum stroke is too short 72 Pump drive stop notfound 73 Timeout Wait for Composition 73 Timeout Wait for run volume 74 Timeout Wait for Volume 74 Timeout Wait for Flow 75 Timeout Wait for Pressure 75 Drive Encoder failed 76 Drive phases differ too much in electric resistance 76 Degasser s pressure limit violation 77 Seal wash pump was missing when tried to turnon 77 Valve hardware overcurrent MCGV 78 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 52 Agilent 1290 Infinity II Flexible Pump User Manual Error Information 6 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow w
113. plied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 01046 60105 Remote cables p n 5188 8029 5188 8044 5188 8045 5061 3378 01046 60201 CAN cables p n 5181 1516 5181 1519 162 Description Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description ERI to general purpose Remote Cable ERI ERI Remote Cable APG ERI Remote Cable to 35900 A D converter Agilent module to general purpose Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Agilent 1290 Infinity II Flexible Pump User Manual LAN cables p n 5023 0203 5023 0202 RS 232 cables not for FUSION p n board G1530 60600 RS232 61601 5181 1561 USB cables p n 5188 8050 5188 8049 Identifying Cables 9 Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m Description USB A M USB Mini B 3 m PC Module US
114. pump head use pressurized air 1 2 and 3 Pump head parts in contact with the piston seal need to be cleaned properly in order to get a smooth surface and a tight connection Agilent 1290 Infinity I Flexible Pump User Manual 115 7 Maintenance 7 To remove wear use isopropanol 9 To dry the pump head and to remove residues use 8 To clean the piston and the pump head sufrace use the pressurized air 1 2 and 3 ultra fine abrasive mesh p n 8660 0852 10 If the wash seal shall be replaced by a new one use the steel side of the insert tool for removing it Wash seal 116 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Replacing the Heat Exchanger Tools required Parts required Preparations CAUTION Description Wrench 19 mm p n Description G4220 81013 Heat Exchanger secondary pump head only G4220 20028 Headless screw for 1290 Infinity pump heads G4220 20001 Spacer Fitting Remove the pump head assembly from the pump Remove the secondary pump head from the link plate Loss of small spacer fitting Inside the secondary pump head is a small spacer fitting which can be dropped easily when removing the heat exchanger gt The heat exchanger does not need to be removed for pump head maintenance pump head 1 Remove the 19 mm screw at the front of the secondary 2 Remove the front plate Agilent 12
115. rature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 26 Agilent 1290 Infinity II Flexible Pump User Manual Physical Specifications Site Requirements and Specifications 2 Table 1 Physical Specifications Type Specification Comments Weight 16 1 kg 35 5 Ibs Dimensions 180 x 396 x 436 mm height x width x depth 7 1 x 15 6 x 17 2 inches Line voltage 100 240 V 10 Wide ranging capability Line frequency Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC EN CSA UL 50 or 60 Hz 5 120 VA 110 W 4 55 C 39 131 F 40 70 C 40 158 F lt 95 r h at 40 C 104 F Non condensing Up to 3000 m 9842 ft Up to 4600 m 15092 ft For storing the module Installation category II Pollution degree2 For indoor use only Agilent 1290 Infinity II Flexible Pump User Manual 27 2 Site Requirements and Specifications Performance Specifications 28 Table 2 Agilent 1290 Infinity Il Flexible Pump G7104A Performance Specifications Feature Specification Hydraulic system Pump resolution step size Settable flow range Flow precision Flow accuracy Pressure range Pressure pulsation Compr
116. rd LAN cable MAC label LAN interface Figure 24 Location of LAN interfaces and MAC label 190 Agilent 1290 Infinity I Flexible Pump User Manual LAN Configuration 11 TCP IP Parameter Configuration To operate properly in a network environment the LAN interface must be configured with valid TCP IP network parameters These parameters are e IP address e Subnet Mask Default Gateway The TCP IP parameters can be configured by the following methods e by automatically requesting the parameters from a network based BOOTP Server using the so called Bootstrap Protocol by automatically requesting the parameters from a network based DHCP Server using the so called Dynamic Host Configuration Protocol This mode requires a LAN onboard Module or a G1369C LAN Interface card see Setup DHCP on page 198 e by manually setting the parameters using Telnet e by manually setting the parameters using the Instant Pilot G4208A The LAN interface differentiates between several initialization modes The initialization mode short form init mode defines how to determine the active TCP IP parameters after power on The parameters may be derived from a Bootp cycle non volatile memory or initialized with known default values The initialization mode is selected by the configuration switch see Table 11 on page 193 Agilent 1290 Infinity II Flexible Pump User Manual 191 11 LAN Configuration Configuration Swi
117. re Setup 216 ambient non operating temperature 27 ambient operating temperature 27 analog signal 182 apgremote 182 assemble primary pump head 122 secondary pump head 122 automatic configuration with Bootp 201 bench space 26 BootP service installation 203 restart 210 settings 209 stop 209 Bootp amp Store 194 automatic configuration 201 initialization modes 193 using default 195 using stored 195 230 c cable CAN 170 LAN 170 overview 162 RS 232 171 cables analog 164 remote 166 CAN cable 170 capillaries 86 cleaning 85 compensation sensor open 57 compensation sensor short 58 condensation 26 configuration switch 192 D defect connection between main board and pump drive encoder 67 degasser s pressure limit violation 77 delay volume description 40 deliver underrun 67 DHCP general information 197 setup 198 dimensions 27 disassemble primary pump head 111 pump head assembly 108 secondary pump head 114 doors install 87 remove 87 drive current too high 65 drive current too low 65 drive encoder error 70 drive encoder failed 76 drive phases differ too much in electric esistance 76 drive position limit 70 drive timeout 66 E electrical connections descriptions of 177 electronic waste 226 EMF early maintenance feedback 187 error messages compensation sensor open 5 7 compensation sensor short 58 defect connection between main board and pump drive encoder 67 degasser s pressure
118. rews are loose e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Wiper shifted Please contact your Agilent service representative 2 Pump head blocks piston movement Replace clean or repair pump head 3 Pump drive motor is mechanically blocked Please contact your Agilent service representative Agilent 1290 Infinity II Flexible Pump User Manual Error Information 6 Pump drive stop not found Error ID 29207 The pump drive stop has not been found Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Pump drive spindle is defect Please contact your Agilent service representative Timeout Wait for Composition Error ID 29180 A target condition composition has been sent to the instrument which should have been reached within an expected time frame but didn t Either the limit time frame or the current value of the variable has been modified later directly or indirectly Probable cause Suggested actions 1 Incorrect parameters have been sent tothe Verify control software macros manual instrument by the control software or commands manual changes Agilent 1290 Infinity II Flexible Pump User Manual 73 74 Error Information Timeout Wait for run volume Error ID 29181 A target condition run volume which is the volume delivered since the method run start has been sent to the instrument which should have been reached within an expected time fram
119. sembling the pump head remove the 4 hex 2 Remove the front part of the pump head including pump screws at the rear of the pump head chamber housing with pump seal distance sheet support ring seal holder including backup ring and the gasket Support ring fi l eal wash gasket AN Seal holder K s Distance sheet R i OY Pump chamber b housing a J I ey SO ST gw wa Agilent 1290 Infinity I Flexible Pump User Manual 111 7 Maintenance 3 Remove the piston from the piston housing by pushing it to the rear then pull it out from the rear 4 Check the pump pistons for scratches grooves and dents Piston surface 5 Use the steel side of the insert tool for removing the pump seal from the pump chamber housing Pump seal Do not use the pump piston for that purpose as this can break it 6 To flush out particles from the pump head use pressurized air 1 2 and 3 Pump head parts in contact with the piston seal need to be cleaned properly in order to get a smooth surface and a tight connection 112 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 To remove wear use isopropanol 8 To clean the piston and the pump head sufrace use the ultra fine abrasive mesh p n 8660 0852 10 If the wash seal shall be replaced by a new one use the steel side of the insert tool for removing it Wash seal 9 To dry the pump head and
120. sembly CAUTION Damage of connections Disassembling the flow connection between the two pump heads of the pump head assembly solvent channel can damage the connection and cause leaks gt Do not disconnect the flow connection between the pump heads CAUTION Damage of internal parts gt Do not apply a strong force to the screws of the pump head gt Use a torque hex key for that purpose CAUTION Damage of the pump piston Removing pump heads in a position other than the maintenance position can damage the pump piston gt Before switching off the pump bring it to the maintenance position CAUTION Damage of pump drives The pump drive can be damaged if the pump initializes after switching it on without having the pump head installed properly gt Use the Lab Advisor maintenance procedure for replacing pump heads gt Install the pump head correctly before switching on the pump Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 VTE Tm Damaged pump head Disassembling or reassembling the pump head with tools other than the ones recommended can damage pump heads and significantly reduce their life time gt Follow all instructions step by step gt Use recommended tools like the pump head alignment tool and a torque wrench INTET Damage of pump piston The pump piston is made of Zr0 based ceramic which is a very hard and resistant material but it is sensitive to sh
121. sing Internal 4 solvent gradient formation valve included External 2x 12 solvent valve as option fully integrated in the pump control interface Integrated Number of channels 4 Internal volume per channel 1 5 mL TFE PDD copolymer FEP PEEK PPS stainless steel polyimide Included allows automatic inline filter back flush and automatic mixer change e g for optional TFA mixer Included Included Controller area network CAN RS232C APG remote ready start stop and shutdown signals LAN Extensive diagnostics error detection and display through included Agilent LabAdvisor leak detection safe leak handling leak output signal for shutdown of the pumping system Low voltage in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre defined and user settable limits and feedback messages Electronic records of maintenance and errors All materials are recyclable Agilent 1290 Infinity II Flexible Pump User Manual 29 2 Site Requirements and Specifications Performance Specifications 30 Agilent 1290 Infinity II Flexible Pump User Manual Agilent 1290 Infinity Il Flexible Pump User Manual c00 ee 3 7 e Using the Pump i Magnets 32 Turn on off 33 Status Indicators 34 Best Practices 35 Daily Weekly tasks 35 Power up Shut down the pump 35 Prepare the pump 36 How to deal wit
122. sor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative 56 Agilent 1290 Infinity II Flexible Pump User Manual Error Information 6 Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the Power Switch board Please contact your Agilent service representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being pinched by a metal component Please contact your Agilent service representative Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause S
123. specified by the manufacturer The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions 220 Agilent 1290 Infinity II Flexible Pump User Manual Appendix 12 Before Applying Power WARNING Wrong voltage range frequency or cabling Personal injury or damage to the instrument gt Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations gt Make all connections to the unit before applying power Note the instrument s external markings described under Safety Symbols on page 224 Ground the Instrument WARNING Missing electrical ground Electrical shock gt If your product is provided with a grounding type power plug the instrument chassis and cover must be connected to an electrical ground to minimize shock hazard gt The ground pin must be firmly connected to an electrical ground safety ground terminal at the power outlet Any interruption of the protective grounding conductor or disconnection of the protective earth terminal will cause a potential shock hazard that could result in personal injury Agilent 1290 Infinity II Flexible Pump User Manual 221 12 Appendix Do Not Operate in an Explosive Atmo
124. sphere Presence of flammable gases or fumes Explosion hazard gt Do not operate the instrument in the presence of flammable gases or fumes Do Not Remove the Instrument Cover WARNING Instrument covers removed Electrical shock gt Do Not Remove the Instrument Cover gt Only Agilent authorized personnel are allowed to remove instrument covers Always disconnect the power cables and any external circuits before removing the instrument cover Do Not Modify the Instrument Do not install substitute parts or perform any unauthorized modification to the product Return the product to an Agilent Sales and Service Office for service and repair to ensure that safety features are maintained In Case of Damage WARNING Damage to the module Personal injury for example electrical shock intoxication gt Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel 222 Agilent 1290 Infinity II Flexible Pump User Manual Appendix 12 Solvents WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet
125. tP Addresses The Edit BootP Addresses screen appears 4 Click Add The Add BootP Entry screen appears Add BootP Entry x Figure 34 Enable BootP logging Agilent 1290 Infinity II Flexible Pump User Manual 207 11 LAN Configuration 5 Make these entries for the instrument e MAC address Host name Enter a Hostname of your choice The Host Name must begin with alpha characters i e LC1260 e IP address Comment optional e Subnet mask e Gateway address optional The configuration information entered is saved in the Tab File Click OK Leave Edit BootP Addresses by pressing Close Exit BootP Settings by pressing OK After each modification of the BootP settings G e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service on page 209 or Restarting the Agilent BootP Service on page 210 oo N O 10 Power cycle the Instrument OR If you changed the IP address power cycle the instrument for the changes to take effect 11 Use the PING utility to verify connectivity by opening a command window and typing Ping 192 168 254 11 for example The Tab File is located at C Documents and Settings All Users Application Data Agilent BootP TabFile 208 Agilent 1290 Infinity II Flexible Pump User Manual LAN Configuration 11 Changing the IP Address of an Instrument Using the Agilent BootP Service Agilent BootP Servi
126. tP Service 201 How BootP Service Works 202 Situation Cannot Establish LAN Communication 202 Installation of BootP Service 203 Two Methods to Determine the MAC Address 205 Assigning IP Addresses Using the Agilent BootP Service 206 Changing the IP Address of an Instrument Using the Agilent BootP Service 209 Manual Configuration 211 With Telnet 212 PC and User Interface Software Setup Setup 216 PC Setup for Local Configuration 216 User Interface Software Setup 217 This chapter provides information on connecting the module to the controller software Apg Agilent Technologies 189 11 LAN Configuration What You Have To Do First What You Have To Do First The module has an on board LAN communication interface 1 Note the MAC Media Access Control address for further reference The MAC or hardware address of the LAN interfaces is a world wide unique identifier No other network device will have the same hardware address The MAC address can be found on a label at the rear of the module underneath the configuration switch see Figure 24 on page 190 G4204 65800 Part number of the pump main board AA ZZ 210 MSSA Revision code vendor year and week of assembly MAC 003xD31 X6Ex x MAC address Made in Germany Country of origin Figure 23 MAC label 2 Connect the instrument s LAN interface see Figure 24 on page 190 to the PC network card using a crossover network cable point to point or a hub or switch using a standa
127. tch Configuration Switch The configuration switch can be accessed at the rear of the module Configuration switch Figure 25 Location of Configuration Switch The module is shipped with all switches set to OFF as shown above NOTE To perform any LAN configuration SW1 and SW2 must be set to OFF Table 10 Factory Default Settings Initialization Init Mode Bootp all switches down For details see Initialization Mode Selection on page 193 Link Configuration speed and duplex mode determined by auto negotiation for details see Link Configuration Selection on page 200 192 Agilent 1290 Infinity I Flexible Pump User Manual LAN Configuration 11 Initialization Mode Selection Table 11 The following initialization init modes are selectable Initialization Mode Switches SW6 SW7 SW8 Init Mode OFF OFF OFF Bootp OFF OFF ON Bootp amp Store OFF ON OFF Using Stored OFF ON ON Using Default ON OFF OFF DHCP 1 Requires firmware B 06 40 or above Modules without LAN on board see G1369C LAN Interface Card Bootp When the initialization mode Bootp is selected the module tries to download the parameters from a Bootp Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the module Therefore the parameters are lost with the next power cycle of the module Bootp gt Active Parameter Server Figure 26 Boo
128. tion Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Agilent 1290 Infinity II Flexible Pump User Manual 181 10 Hardware Information 182 Instrument PC DCD 1 1 DCD RX 2 2 RX TX 3 A 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 ie 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 21 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START
129. tname resolution e g NetBIOS DHCP Active Server Parameter Figure 30 DHCP Principle 1 It may take some time until the DHCP server has updated the DNS server with the hostname information 2 It may be necessary to fully qualify the hostname with the DNS suffix e g 0030d3177321 country company com 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions Agilent 1290 Infinity II Flexible Pump User Manual 197 11 LAN Configuration Setup DHCP Software required The modules in the stack must have at least firmware from set A 06 34 and the above mentioned modules B 06 40 or above must from the same firmware set 1 Note the MAC address of the LAN interface provided with G1369C LAN Interface Card or Main Board This MAC address is on a label on the card or at the rear of the main board e g 0030d3177321 On the Instant Pilot the MAC address can be found under Details in the LAN section System Info Controller DE12345678 G4208A Main Revision B 02 12 0001 DAD DE64260019 G1315D Main Revision B 06 41 0002 a Resident Revison _B 06 40 0007 A print On time 3d 01 33h Installed Options Dhcp LAN TCP IP Mode DHCP LAN TCP IP Address 130 168 132 219 LAN MAC Address _0030D314F89E Board ID TYPE G1315 66565 SER MAC REV AC MFG Lamp 2140 0820 848728 Cell no info Exit v information on each module a
130. to remove residues use pressurized air 1 2 and 3 Agilent 1290 Infinity I Flexible Pump User Manual 113 7 Maintenance Disassemble the Secondary Pump Head INE Damage of pump piston The pump piston is made of Zr07 based ceramic which is a very hard and resistant material but it is sensitive to shearing forces from the side gt Do not try to remove the pump piston from the rear gt Do not use the piston for removing pump seals 1 For disassembling the pump head remove the 4 hex 2 Remove the front part of the pump head including pump screws at the rear of the pump head chamber housing distance sheet pump seal and seal holder and support ring with wash seal and gasket upport ring S gt Seal wash gasket gt LNA istance sheet s A W Pump chamber housing 114 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 3 Remove the piston from the piston housing by pushing it to the rear then pull it out from the rear 4 Check the pump pistons for scratches grooves and dents when changing the piston seals Damaged pistons cause micro leaks and will decrease the lifetime of the seals 5 Use the steel side of the insert tool for removing the pump seal from the pump chamber housing cD Insert tool Pump seal Do not use the pump piston for that purpose as this can break it 6 To flush out particles from the
131. tp Principle Agilent 1290 Infinity II Flexible Pump User Manual 193 11 LAN Configuration 194 Bootp amp Store When Bootp amp Store is selected the parameters obtained from a Bootp Server become the active parameters immediately In addition they are stored to the non volatile memory of the module Thus after a power cycle they are still available This enables a kind of bootp once configuration of the module Example The user may not want to have a Bootp Server be active in his network all the time But on the other side he may not have any other configuration method than Bootp In this case he starts the Bootp Server temporarily powers on the module using the initialization mode Bootp amp Store waits for the Bootp cycle to be completed closes the Bootp Server and powers off the module Then he selects the initialization mode Using Stored and powers on the module again From now on he is able to establish the TCP IP connection to the module with the parameters obtained in that single Bootp cycle Bootp Active Server Parameter Non Volatile RAM Stored Parameter Figure 27 Bootp amp Store Principle Use the initialization mode Bootp amp Store carefully because writing to the non volatile memory takes time Therefore when the module shall obtain its parameters from a Bootp Server every time it is powered on the recommended initialization mode is Bootp Agilent 1290 Infinity II Flexible Pu
132. uggested actions 1 Loose connection between the power switch board and the main board 2 Defective power switch board Agilent 1290 Infinity II Flexible Pump User Manual Please contact your Agilent service representative Please contact your Agilent service representative 57 58 Error Information Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective power switch board Please contact your Agilent service representative 2 Loose connection between the power Please contact your Agilent service switch board and the main board representative Agilent 1290 Infinity II Flexible Pump User Manual Error Information 6 Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated Depending on the module assemblies e g the lamp in the detector are turned off to assure
133. urpose Valve Normal Operating Mode With Mixer In this mode the flow passes an optional Jet Weaver and the inline filter This configuration is recommended for special applications which require an increased mixing efficiency From pump Jet Weaver To system Inline filter Figure 5 Valve position in normal operating mode with mixer 16 Agilent 1290 Infinity II Flexible Pump User Manual Introduction 1 Positions of the Multi Purpose Valve Filter Flush Mode This mode is used for cleaning the inline filter by back flushing it The flow goes to port 5 passes the inline filter in opposite direction and leaves to the waste through port 7 From pump To waste Inline filter Figure 6 Valve position in filter flush mode Agilent 1290 Infinity I Flexible Pump User Manual 17 1 Introduction Positions of the Multi Purpose Valve Service Mode In service mode the flow is diverted to port 3 where for example a restriction capillary can be installed for diagnostic tests From pump Restriction capillary A Figure 7 Valve position in service mode 18 Agilent 1290 Infinity II Flexible Pump User Manual Introduction 1 Leak and Waste Handling Leak and Waste Handling The 1290 Infinity II Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed i An Figure 8 Leak and waste handling conc
134. ve to Inline Filter Waste Tubing with Fitting Capillary ST 0 17 mmx900 mm SI SX ps ps Pump to Multisampler Capillary ST 0 17 x 300 mm S SX Capillary ST 0 17 x 120 mm SLV SV for Jet Weaver not shown Agilent 1290 Infinity II Flexible Pump User Manual 147 8 Parts and Materials Pump Head Assembly Parts Pump Head Assembly Parts 148 Agilent 1290 Infinity I Flexible Pump User Manual Item p n 1 G4220 81013 2 G4220 60022 3 G4220 60028 4 G4220 20020 5 Parts and Materials Description Heat Exchanger secondary pump head only Channel A Inlet valve primary pump head Outlet valve primary pump head Internal gold seal for Outlet Valve Adapter Agilent 1290 Infinity II Flexible Pump User Manual 149 8 Parts and Materials Primary Pump Head Parts Primary Pump Head Parts 10 OP 2 Figure 13 Primary pump head parts 150 Agilent 1290 Infinity II Flexible Pump User Manual Parts and Materials 8 Primary Head G4204 60060 Item p n Description 1 0515 1218 Screw M5 40 mm long 2 64204 60033 PH Body prim 3 0905 1719 Metering seal 100 pL 4 5023 2513 Distance sheet 5 G4220 60016 Seal holder including backup ring 6 01018 07102 Gasket Seal wash 7 0905 1718 Backup Seal PE Seal Wash 8 G4220 63010 Support Ring Seal Wash 9 G4220 23705 Bracket 10 G4220 60000 Preload Assy 11 5067 5938 Plunger Agilent 1290 Infinity II Flexible Pump User Manual 151 8 Parts and Materials Se
135. ver 90 Replace the Inlet Valve 92 Replace the Outlet Valve 93 Remove the Jet Weaver 95 Install the Jet Weaver 97 Replace the Seal Wash Pump 99 Replace the Multi Channel Gradient Valve MCGV 100 Release a Stuck Inlet Valve 102 Pump Head Procedures 104 Remove the Pump Head Assembly 106 Disassemble the Pump Head Assembly 108 Disassemble the Primary Pump Head 111 Disassemble the Secondary Pump Head 114 Replacing the Heat Exchanger 117 Replace Wash Seal and Gasket 120 Assemble the Primary and Secondary Pump Head 122 Reassemble the Pump Head Assembly 130 Install the Pump Head Assembly 131 Replace the Multi Purpose Valve 133 Apg Agilent Technologies 79 7 Maintenance Replace Parts of the Multi Purpose Valve 135 Replace the High Pressure Outlet Filter or Filter Frit 136 Replace Parts of the Inline Filter 138 Replace the Module Firmware 140 Prepare the Pump Module for Transport 141 This chapter describes the maintenance of the Agilent 1290 Infinity II Flexible Pump 80 Agilent 1290 Infinity II Flexible Pump User Manual Maintenance 7 Introduction to Maintenance Introduction to Maintenance Figure 10 on page 81 shows the main user accessible assemblies of the Agilent 1290 Infinity II Flexible Pump These parts can be accessed from the front simple repairs and don t require to remove the pump from the system stack Seal Wash Sensor High Pressure Filter Housing Jet Weaver optional Seal Wash Pump Degass
136. wing at the system DOS prompt c gt telnet lt IP address gt or c gt telnet lt host name gt E C AWINDOWS system32 cmd _exe C gt telnet 134 46 27 95 Figure 38 Telnet Starting a session where lt IP address gt may be the assigned address from a Bootp cycle a configuration session with the Handheld Controller or the default IP address see Configuration Switch on page 192 When the connection was established successfully the module responds with the following ET C AWINDOWS system32 cmd exe telnet 134 40 27 95 Agilent Technologies G1i315C PP 8000624 Figure 39 A connection to the module is made Agilent 1290 Infinity II Flexible Pump User Manual LAN Configuration 11 3 Type and press enter to see the available commands WINDOWS system32 cmd_exe telnet 134 40 27 95 Agilent Technologies G1315C PP 8880824 gt command syntax description display help info display current LAN settings ip lt x x x x gt set IP Address sm X X X X gt set Subnet Mask To gt a gt ab O27 set Default Gateway exit exit shell gt Figure 40 Telnet Commands Table 16 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip lt X X X xX gt sets new ip address sm lt X X X X gt sets new subnet mask gw lt X X X x gt sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows th

Agilent 1290 Infinity II Flexible Pump User Manual

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