Perkin Elmer Lambda UV
Contents
1. B Cancel 2 Select whether Games are turned available using the left and right arrow keys The options are Yes or No 3 Define the Theme the onscreen layout of folders The options are either a Grid format or a List 4 Select the History mode using the left and right arrow keys If Off is selected the instrument will not remember your method settings unless you restore them as a new method The default is Off 5 Select whether to use an Auto Standby mode after defined periods The options are 1 hour 2 hours At Night or Off 6 Press OK gt to store the settings and return to the Utilities folder Contrast Ambient temperature can affect the display The contrast function can optimize the display for local conditions Using your Instrument 35 The procedure is as follows 1 Press 5 to select Contrast The Contrast screen is displayed Contrast 2 Adjust the Brightness using the left and right arrows and then press the down arrow 3 Adjust the Contrast using the left and right arrows 4 Press OK gt to store the settings and return to the Utilities folder About The About screen displays the instrument serial number and the software version 1 Press 6 to select About The About screen for your instrument is displayed 2 Press OK to close the window and return to the Utilities folder 36 Lambda XLS XLS User s Guide Sample Handling The light beam is directed from2. Standard Curve Standards 10 Enter the concentration values for each standard using the keypad numbers and the up and down arrows to move between the different standard boxes The available range is from 0 001 to 9999 11 Press Next The Standard Curve Calibration screen is displayed Standard Curve Calibration 25 NOTE If any duplicate or non monotonic entries are entered on the Standards screen when Next is pressed the instrument will beep and highlight the incorrect entry 12 13 14 Instrument Methods 57 0A If you selected Standards insert the reference sample and then press E This reference measurement will be used for all subsequent samples until repeated OR If you selected Manual enter the known absorbance value for each concentration using the keypad Go to Step 15 The available range is from 0 001 to 9999 Insert your first standard and press mn If there are any replicates press Replicates to view them then press to measure each replicate Standard Curve Calibration 25 Repeat Step 13 for all your standards A graph will display the results and the curve fit for example zero regression as the measurements are made Standard Curve Calibration 10 0 0 0124 200 0 121 A i 31 0 0 245 A 620 0 370 A 58 Lambda XLS XLS User s Guide NOTE You can use the up and down arrows to select a standar
3. or 4 Press OK to store the settings and return to the Utilities folder Printer 1 Press 3 to select Printer The Printer screen is displayed Printer SD Memory Card B Cancel 2 Select whether Auto Print is On or Off using the left and right arrows and then press the down arrow When Auto Print is On the results are automatically printed after a measurement is taken you exit the application depending on the method When Auto Print is Off printing has to be initiated manually 3 Select how the data are sent The options are Built in the internal printer Computer USB to a computer via USB port SD Memory Card to the SD card or Computer Bluetooth to a computer via Bluetooth The options available depend on what is installed in your instrument NOTE If an SD Memory Card is installed in your instrument the LED next to the card will be lit while data is being printed to the card Removing the card while the LED is lit could corrupt your data For some methods you have to exit the method to end printing See the Lambda Bio XLS Print Utility User s Guide L6050015 on the Lambda Bio XLS User Manuals CD L6050018 for more information 4 Press OK to store the settings and return to the Utilities folder 34 Lambda XLS XLS User s Guide Preferences 1 Press 4 to select Preferences The Preferences screen is displayed Preferences Auto Standby Off
4. AA min x factor and correlation coefficient calculated from a best fit of the data points are displayed They may not be relevant for simple kinetics experiments The procedure to define a new method is as follows 1 Press 1 to open the Standard Methods folder The Standard Methods screen is displayed Standard Methods O ae waveienan Q O Kinetics 3s A e X Multi wavelength Absorbance Ratio e 9 a Spectrum Q Nu Concentration 9 PA Standard Curve Instrument Methods 61 2 Press 4 to open the Kinetics method The Kinetics Method Settings 1 screen is displayed Kinetics Method Settings 1 Wavelength Delay Time 1 Minute 10 Seconds B Cancel Enter all numerical values using the keypad numbers or the left and right arrows 3 Set the Wavelength by using the keypad numbers or left and right arrows then press the down arrow The default value is 340 nm 4 Enter the Delay Time in seconds before measurements are taken then press the down arrow This can be a maximum of 600 seconds 10 minutes 5 Enter the time in minutes over which measurements are taken Duration then press the down arrow This can be a maximum of 60 minutes 6 Enter the Interval time in seconds between measurements using the left and right arrows then press the down arrow The options are 1 2 5 10 15 20 30 or 60 seconds 7 Press Next The Kinetics Method Setti
5. L7110232 Lambda Bio XLS Spare Printer Paper 20 Rolls L7110233 Lambda Bio XLS Bluetooth Accessory A range of cells and microcells are also available from PerkinElmer that are suitable for use with the Lambda XLS XLS instrument Go to the PerkinElmer website and select UV Vis Accessories amp Consumables under UV Vis in LAS Products and Services Any of the 10 mm cells found there will be suitable for use with the Lambda XLS XLS instrument Accessories 71 Printer Installation and Configuration The Printer Accessory L7110230 is an optional accessory that allows you to print your data as you run your samples directly from your instrument Printer installation 1 Turn off the line power supply and disconnect the power cable 2 Turn the instrument over and remove the two cap head screws using the 3 mm hexagonal wrench provided Cap head screws 3 Turn the instrument back over and lift the accessory cover vertically upwards to remove Remove the tie wrap from the cable _ SS EET 4 Invert the instrument and replace the cap head screws 72 Lambda XLS XLS User s Guide 5 Plug the accessory cable into the printer Fa Locating bosses Printer Accessory cable 6 Lower the printer onto the locating bosses and push down firmly 7 Reconnect the power cable Printer configuration 1 Switch the instrument on and then open the Utilities folder 2 Press 3 to open the Printer folder 3 Select Built
6. 12 Press 3 to open the Printer folder 13 Select Computer Bluetooth as the Printer option using the arrow keys Printer Auto Print Printer 4Computer Bluetooth 14 Press OK gt to save the settings Accessories 77 Lambda Bio XLS Print Utility There are two software applications comprising the Lambda Bio XLS Print Utility The Lambda Bio XLS Report Utility is an application running separately under Windows XP that enables you to connect via a USB cable to a PC onto which the software has been installed You can connect to several instruments simultaneously limited only by hardware and the speed of the host system Once your data is on the PC it can be printed directly to any printer that is connected to that PC or saved The Report Utility can store data either to a common directory or be configured to save to independent directories by both file format and connection It can save data as an Enhanced Metafile emf graphics file a comma delimited csv data file a tab delimited txt data file or as an Excel file or in print utility pvc format for viewing using the Lambda Bio XLS Report Viewer NOTE An internal printer is also available for the instrument This is either supplied pre installed in the instrument or as an optional accessory L7110230 If you have this option please see the Lambda Bio XLS Print Utility User s Guide L6050015 on the Lambda Bio XLS User Manuals CD L6050018 for more in
7. RIGHT to LEFT through the cell chamber as indicated by the white arrows on the instrument enclosure Make sure that the cell is inserted in the correct alignment The cell holder supplied with the instrument accepts standard 10 40mm Lambda XLS or 10 mm Lambda XLS pathlength quartz glass or plastic cells The optical height is 15 mm and the minimum volume that can be used is approximately 70 ul in a micro cell 12 mm test tubes may be used for example for cell cultures However they are not recommended as higher quality data is produced by using disposable cuvettes for the analysis If used align the indicator line on 12 mm test tubes in the same direction to ensure reproducible positioning of the tube Note that test tubes do not last forever and that the surface becomes scratched and blemished through repetitive use if this is the case they should be replaced NOTE When using calibration standard filters insert such that the flat surface is facing away from the spring end of the cell holder NOTE A cover is provided to keep the sample compartment free from dust and debris When the instrument is in use the dust cover should be kept in a safe place nearby Instrument Methods 38 Lambda XLS XLS User s Guide Single Wavelength The Single Wavelength method makes absorbance A and percentage transmission T measurements on samples by measuring the amount of light that has passed throug
8. Unpacking and Installation e This equipment is designed to be installed in accordance with the Canadian Electrical Code CEC Part 1 CSA C22 1 and CSA C22 2 No 0 e Inspect the instrument for any signs of damage caused in transit If any damage is discovered access the Troubleshooting Guide on the PerkinElmer website http www perkinelmer com and follow the process there If you do not have access to the website contact Customer Care see Appendix 2 Customer Care Contacts on page 85 e Ensure your proposed installation site conforms to the environmental conditions for safe operation Indoor use only Temperature range 5 C to 35 C Maximum relative humidity of 80 up to 31 C decreasing linearly to 50 at 40 C NOTE If you use the instrument in a room subjected to extremes of temperature change during the day it may be necessary to recalibrate by switching off and then on again once thermal equilibrium has been established 2 3 hours e If the instrument has just been unpacked or has been stored in a cold environment it should be allowed to come to thermal equilibrium for 2 3 hours in the laboratory before turning the instrument on This will prevent calibration failure as a result of internal condensation e The instrument must be placed on a stable level bench or table that can take its weight approximately 4 5 kg with enough space around the instrument so that air can circulate freely e This eq
9. Wavelength Method Settings screen is displayed Multi Wavelength Method Settings Wavelengths OK B Cancel 3 Select the number of Wavelengths then press the down arrow The options are between 2 and 5 4 Enter the first wavelength A1 using either the number keys or the left and right arrows then press the down arrow 5 Repeat Step 4 for each of the wavelengths 42 Lambda XLS XLS User s Guide 6 Press OK to start measurements The Multi Wavelength results screen is displayed Multi Wavelength Sample T EE 280 300 320 340 360 380 400 Man 0A Ua 7 Insert the Reference sample and press d This reference measurement will be used for all subsequent samples until repeated 8 Insert your first sample and press mn Multi Wavelength Sample um 300 320 340 360 380 400 20 A spectrum covering the range of wavelengths is displayed with cursors marking the chosen wavelengths A table of the absorbance values at those wavelengths is also displayed 9 Repeat Step 8 for each sample Instrument Methods 43 Press to display the available Options which you can select using the keypad numbers Method Settings Returns to the Method Settings screen Print Prints the data on the results screen the absorbance table via the selected method Print Graph Prints the graph this option is grayed out if
10. XLS Documentation CD L7111025 Cuvette dust cover L7110231 Lambda Bio XLS Print Utility and Cable if applicable N A Power cable 2 Examine the components for any signs of damage in shipment e Checkthe enclosure of the instrument for damage and make sure that the terminals are not damaged e Make sure that the compartment is free of dust or other foreign matter NOTE In the event of damage or missing parts access the Troubleshooting Guide on the PerkinElmer website http www perkinelmer com and follow the process there If you do not have access to the website contact Customer Care see Appendix 2 Customer Care Contacts on page 85 Installing your Instrument 17 Connecting to the Line Power Supply Electrical Hazard To prevent potential injury to yourself and damage to the instrument make any electrical connections in the system before connecting to the WARNING f line power supply The instrument automatically adjusts to the correct operating voltage gt Connect to the supply outlet with power supply provided and the power cord that is appropriate for your region You must use the power cord and power supply supplied with the instrument NOTE If the instrument has just been unpacked or has been stored in a cold environment it should be allowed to come to thermal equilibrium for 2 3 hours in the laboratory before turning the instrument on This will prevent calibration
11. method setting is Off all method settings and options will return to their default settings when you leave that method unless it has been saved as part of a method gt Press Options W to view the Options menu 1j Method Settinas Fin E Show Graph Run Standard Save Method 3 Auto Print v i Sample Number o Text Function Method Settings Returns to the Method Settings screen for the method Print Prints the results N A Specific to the method Sample Number Defines the sample number you wish to start taking measurements from Save Method Saves the Method Settings as a new method with a defined method name in the Methods or Favorites folder Auto Print Toggles Auto Print on and off The default is off Press or O or wait 20 seconds to exit the method options You can use the numeric keys as a shortcut to the option reguired without needing to enter the Options menu 26 Lambda XLS XLS User s Guide Methods and Favorites The Methods folder allows you to store user modified methods based on the default instrument methods which can be saved using the Options menu Up to 9 methods can be saved in each folder To retrieve previously stored methods press the appropriate keypad number to enter the Methods folder You can also import methods from an SD card or export methods to an SD card Methods Figure 7 Method
12. no data are available Sample Number Adds a prefix to the sample number and resets the incrementing number to the desired value Save Method Use the left and right arrows to select a folder to store the method in Favorites Methods 1 9 then press the down arrow and enter a name for the method Press Save to save the method v Auto Print Toggles Auto Print on and off Press or O or wait 20 seconds to exit the method options Press EI to return to the Standard Methods folder after all your samples have been measured 44 Lambda XLS XLS User s Guide Spectrum The Spectrum method enables you to collect an absorption spectrum and determine peak heights and positions The procedure is as follows 1 Press 1 to open the Standard Methods folder The Standard Methods screen is displayed Standard Methods o A wavelength 3 O Kinetics uas X e X Multi wavelength Absorbance Ratio AA Spectrum O Concentration 9 E Standard Curve 2 Press 3 to open the Spectrum method The Spectrum Method Settings screen is displayed Spectrum Method Settings Start Wavelength End Wavelength 500 nm Absorbance E OK B Cancel 3 Setthe Start Wavelength by using the keypad numbers or the left and right arrows then press the down arrow 4 Setthe End Wavelength by using the keypad numbers or the left and right arrows then press the d
13. options which you can select using the keypad numbers Method Settings Returns to the Method Settings screen Print Prints the data on the results screen via selected method Print Data Prints all the data Set ty at cursor Sets the t position the starting point for the slope and dA calculations at the current cursor position The value is retained for subsequent samples Set t At Cursor Sets the t position the finishing point for the slope and dA calculations at the current cursor position The value is retained for subsequent samples Show Slope Toggles the calculated slope line on and off NOTE If any data points enclosed by ty and tn are beyond the range of the instrument that is 22 5 A or lt 0 3 A then this option is grayed out Sample Number Adds a prefix to the sample number and resets the incrementing number to the desired value Save Method Use the left and right arrows to select a folder to store the method in Favorites Methods 1 9 then press the down arrow and enter a name for the method Press Save to save the method Auto print Toggles Auto Print on and off Press or or wait 20 seconds to exit the method options Press O to return to the Standard Methods folder after all your samples have been measured Instrument Methods 65 Absorbance Ratio The Absorbance Ratio method makes simple absorbance ratio measurements on samples measuring the amo
14. pg ml ug yl pmol ul mg dl mmol l pmol l g l mg l ug l U I ppm ppb conc or none The Units screen also enables you to select the number of displayed decimal points DP The options are Auto 0 1 and 2 NOTE The result will always be fixed to 5 significant figures regardless of how many decimal points are selected so 98768 2 is displayed as 98768 even with 1 decimal point selected Press OK to save your options and return to the Method Settings screen The units can still be edited 7 Press OK gt to start measurements The Concentration results screen is displayed Concentration wavelength Sample 260 nm Absorbance Concentration Factor 50 0 0A os 8 Insert the Reference sample and press a This reference measurement will be used for all subsequent samples until repeated Instrument Methods 51 9 If you selected Measure Standard insert your standard and press OK mn The Run Standard screen is displayed Run Standard B Cancel OR If you selected Factor go to Step 11 52 Lambda XLS XLS User s Guide 10 Press Run gt to run the standard The Factor is calculated and displayed Concentration Wavelength Sample 260 nm Standard Absorbance Concentration 50 0 8333 11 Insert your first sample and press gt The Concentratio
15. symbols use the left right Q to select the required setting 24 Lambda XLS XLS User s Guide Taking measurements After the method settings have been entered press OK to start measurements The results screen for the method is displayed Single Wavelength Sample 620 nm Result Figure 5 A typical results screen 0A 1 Insert the Reference sample in the sample holder and press OA 100 T Mua to take the reference measurement 2 Insert your first sample and press 3 Repeat Step 2 for each sample Results The results are displayed on screen Results shown as indicate that the concentration is out of range for example if a negative absorbance value is obtained Single Wavelength Sample 1 A 620 nm Result M 0 038 Units Figure 6 The Single Wavelength method results screen Using your Instrument 25 Options Within each method you are able to select various options that define the way results are treated These options may be specific to the method allow you to print the results or view method settings If you are not using a previously stored method it is advisable to check that these options have been appropriately set for your experiment before running your samples NOTE Setting the History method setting to On see Preferences on page 34 will cause the instrument to store its last settings If the History
16. turning them anticlockwise The thumbscrews remain attached to the plate 2 Lower the sample holder out of the instrument Maintenance 81 Thumbscrews Figure 10 Position of cell holder thumbscrews 62 Lambda XLS XLS User s Guide Storage and Shipping This unit should be stored or shipped in its original packaging which should be kept for this purpose The unit should be kept in its packaging in a dry non condensing environment free from dust or dirt in the range 10 to 50 C Should you need to return your instrument to PerkinElmer a decontamination certificate is required before the instrument is shipped Decontamination certificates can be obtained from PerkinElmer The Troubleshooting Guide which will help you work through the returns process can be accessed at http www perkinelmer com If you do not have access to the Troubleshooting Guide you can also contact Customer Care see Appendix 2 Customer Care on page 85 for more information It is the responsibility of the user to carry out appropriate risk assessments and if applicable decontamination procedures before returning the instrument Appendices 64 Lambda XLS XLS User s Guide Appendix 1 Specifications Specifications are measured after the instrument has warmed up at a constant ambient temperature and are typical of a production unit As part of our policy of continuous development we reserve the right to alter specifications without
17. O o Kinetics A e M Multi Wavelength n Absorbance Ratio om Spectrum Q XD Concentration 5 A Standard Curve 2 Press 4 to open the Concentration method The Concentration Method Settings screen is displayed Concentration Method Settings Wavelength 260 nm Enter Factor B Cancel 3 Enter the Wavelength by using the keypad numbers or the left and right arrows then press the down arrow 50 Lambda XLS XLS User s Guide 4 Select the Mode using the left and right arrows then press the down arrow The options are Enter Factor where a known factor is entered Measure Standard where the factor is calculated from a calibration sample Enter Neg Factor where a known negative factor is entered 5 If you selected Enter Factor or Enter Neg Factor enter the Factor or Negative Factor using the keypad numbers then press the down arrow The available range is from 0 01 to 9999 OR If you selected Measure Standard enter the Concentration using the keypad numbers then press the down arrow The available range is from 0 001 to 9999 6 Enter the Units then press the down arrow You can enter text up to 8 characters long using the keypad or press Options to display the Units screen and access a list of pre defined units In the Units screen use the left and right arrows to select from the available options The options are
18. Software ss 23 Zoom Mode ER ces 48 Utilities EE EE EE Ee 31 Regional AR EE EE EE 32 68 Lambda XLS XLS User s Guide
19. TE If during the calibration the instrument displays an error message it will prompt you to press OK to restart the calibration procedure However after three attempts you will still be able to view the default home page You should NOT use the instrument under these circumstances Instead make a note of the error message and refer to the Troubleshooting Guide on the PerkinElmer website to address the problem Using your Instrument 23 Using the Software The home page contains a list of folders and functions or methods Each item in the list is numbered and is opened by using the associated number on the keypad Opening a folder takes you to another screen of further options Opening a method takes you to a Method Settings screen where you can set the required parameters before using the method to take measurements Concentration Method Settings wavelength 260 nm Enter Factor Press OK to save the selected Method Settings and go on to the next screen Press Cancel to erase selections TE and return to the B Cancel previous screen Figure 4 Features of a typical Method Settings screen Navigation gt Move between settings using the up and down arrows Entering Method Settings Enter Method Settings by using the keypad numbers OR If the box contains the symbol l either type in a value or press options B and choose an option from the next screen OR If the box contains arrow
20. UV VIS SPECTROSCOPY E LAMBDA XLS XLS User s Guide j gt PerkinElmer precisely Release History Part Number Release Publication Date L6050017 A April 2008 Any comments about the documentation for this product should be addressed to User Assistance PerkinElmer Ltd Chalfont Road Seer Green Beaconsfield BUCKS HP9 2FX United Kingdom Or emailed to info perkinelmer com Notices The information contained in this document is subject to change without notice Except as specifically set forth in its terms and conditions of sale PerkinElmer makes no warranty of any kind with regard to this document including but not limited to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior written permission of PerkinElmer Inc Copyright O 2008 PerkinElmer Inc Trademarks Registered names trademarks etc used in this document even when not specifically marked as such are protected by law PerkinElmer is a registered trademark of PerkinElmer Inc Table of Contents I
21. appropriate methods folder 2 Press B to display the available Options 3 Press 1 to select Delete Method 4 Select the method to be deleted using the left and right arrows 5 Press OK to delete the method Lock Method 1 Open the appropriate methods folder 2 Press to display the available Options 3 Press 2 to select Lock Method 4 Select the method to be locked using the left and right arrows then press the down arrow 5 Select a pass code using the keypad numbers or left and right arrows 6 Press OK D to lock the method Unlock Method 1 Open the appropriate methods folder 2 Press to display the available Options 3 Press 3 to select Unlock Method 4 Select the method to be unlocked using the left and right arrows then press the down arrow 5 Enter a pass code using the keypad numbers or left and right arrows Press OK to unlock the method 30 Lambda XLS XLS User s Guide Lambda XLS XLS4 Instrument Methods The pre defined methods available on your Lambda XLS XLS instrument are e Single Wavelength e Multi Wavelength e Spectrum e Concentration e Standard Curve e Kinetics Absorbance Ratio Lambda XLS Spectrometer 13 Ea Standard Methods E Methods Q Eq Utilities 7 274 Figure 8 Lambda XLS home page Using your Instrument 31 Utilities You can use the Utilities folder to modify the instrument setup gt Press the appropriate keypad number to enter t
22. cel Back Stop key Cancels a selection and returns to the previous folder goes Back to previous screen in a method or Stops a measurement Using your Instrument 21 I The OK Next Run Scan key enters or confirms a selection moves to the Next screen or takes a measurement Alphanumeric Use these to enter Method Settings make Options menu selections and to keys write text descriptions where appropriate Use repeated presses to cycle through lower case number and upper case Leave for 1 second before entering the next character Use the key to backspace Press 1 to enter a space 22 Lambda XLS XLS User s Guide Switching on the Instrument NOTE If the instrument has just been unpacked or has been stored in a cold environment it should be allowed to come to thermal equilibrium for 2 3 hours in the laboratory before being switched on This will prevent calibration failure as a result of internal condensation Do not turn power to the instrument on and off quickly as this may CAUTION damage the power supply Wait at least 30 seconds before restarting the instrument gt Switch on the instrument via the ON OFF on the keypad as shown in Figure 2 The instrument will perform a series of diagnostic checks After switch on and calibration the default home page is displayed Lambda XLS Spectrometer E Ea Standard Methods e Q En Utilities Figure 3 Lambda XLS home page NO
23. d to be repeated if a poor reading has been obtained Press e to clear the previous reading before measuring If you selected Replicates when all replicates have been measured press Next gt to return to the Standards screen Standard Curve Calibration Repl 1 0 370 A Repl 2 0 370 A 0 620 0 370 A 15 Press OK to accept the calibration The Standard Curve results screen is displayed Standard Curve Wavelength Sample 430 nm Reference Absorbance Concentration 0 000 A Curve Fit Zero Regression 0A 16 If you selected Manual insert the reference sample and then press E This reference measurement will be used for all subsequent samples until repeated OR If you selected Standards go to Step 17 Instrument Methods 59 17 Insert your first sample and press mn The absorbance of the sample is measured and its concentration is calculated and displayed Standard Curve Wavelength Sample 430 nm 1 Absorbance Concentration 0 1214 1 g Curve Fit a Zero Regression 18 Repeat Step 17 for each sample gt Press NEU to display the available method options which you can select using the keypad numbers Method Settings Returns to the Method Settings screen Print Prints the data on the results screen via selected method Show Graph Toggles the calibration g
24. ds a peak position to the peak table in the results screen at the position set by the cursor The entry in the peak table is then displayed in inverse coloring to discriminate between User Defined Peaks and Auto Detect peaks If the cursor is positioned over a user defined peak the legend User Defined Peak is displayed at the top of the scan The option then changes to Delete Peak to enable you to remove the peak 0 5 0 4 0 3 0 2 0 1 0 0 400 Height 0 0024 width 3 000nm 450 A A 434 521 0 200 Sample 1 A638nm A 0 232 A NOTE Saving a method at this stage will save these user defined wavelengths so that each time the method is run the Absorbance values at these wavelengths are reported 48 Lambda XLS XLS User s Guide Graph Scale Displays the Graph Scale settings screen This enables you to set up a defined graph by defining the limits in either or both of the x and y axes Graph Scale x1 x2 OK B Cancel Zoom Mode This sets up the operation of the Zoom keys up and down arrows The mode x axis zooms the display around the cursor point x axis the mode y axis zooms the display around the cursor point y axis and x amp y axes expands both axes about the cursor point With x or y axis limits set to on zooming out will only be permitted within the set limits x axis limits y axis limits Setting to On act
25. ed emissions This appliance is marked according to the European directive 2002 96 EC on Waste Electrical and Electronic Equipment WEEE See the Lambda Bio XLS Safety Manual L6050014 for more information There are a number of warning labels and symbols on your instrument These are there to inform you where potential danger exists or particular caution is required Before commencing installation please take time to familiarize yourself with these symbols and their meaning This instrument is subject to the following hazards The UV source contained within the unit generates a light beam that traverses the sample chamber during sample measurements Under normal use this beam is contained within the unit and no UV radiation is emitted from the system However care should be taken that no reflective items are placed in the beam during sample measurements so that the beam is reflected out of the system Prolonged exposure to the beam may cause permanent eye damage Identical constraints apply if the sample holder is removed from the unit for cleaning this should only be done with the power disconnected from the instrument e High voltages exist inside the unit Repair and maintenance should only be carried out by individuals trained specifically to work on these instruments Safety Information 11 There are no bio hazardous materials within the unit however this unit could be used with bio hazardous samples Given the genera
26. eese OE EE 28 Lambda XLS XLS Instrument Methods ies ese sees ee RE RA nen 30 Utilities 25 4 HIE EK EE EE N eR EAT RARE 31 Date and Time EE EE egere eto rent ear ero sk ee cue 32 ele EER OR EE ERE ER EEN ER EE ET 32 Ma OE OE OE 33 Preferences d N EE PRO 34 CODLIaSU AE ic ERE CES nene Pere OE EE IL ney 34 pool EE 35 Sample Handling 43 6 VAS ER RES ERENS GEE hae erri RE rtr Rear tn Een RR RR ee EE 36 Instrument Methods en keen RR E RR KERE KEER RR EE REK EERE RR EE RR KEER KERE nnn nnn 37 Sin le Wavelength ss EE tein d oe EE ERENS edi AE DA ED PR ER ER ERA Nee RA 38 AE MAT OR EE ER EE ERR 41 SPECULUM M I 44 Concent atO eee E E 49 Seul 54 Me EE 60 Absorbarnce RatiQ i cor ceo cce een d ere draco ER exe rad 65 ACCESSOFIES qM 69 Accessories and Consumables sceeeeee nnne nnne 70 Printer Installation and Configuration eee 71 Printer installation ecce SE Ede Lora codes re Den rni EERS Oe ene Kees 71 Printer configuration 222 ett eintreten bee see n EE Vee Efe Ke 72 Loading or changing the printer paper iese esse ee ee ee eek AR Ee ee ee 73 Bluetooth Accessory Installation esse ee ee Ee ee EE EE ee nnn 7a Lambda Bio XLS Print Utility ee ee ee ee RE EE ee RE Re ee AE Ee ee AE Ee Re ee AE 77 Maintenance R RR EE REK RE R RR eene naira na aiana RR EE raum REK EER ERK EER EE 79 Ge
27. failure as a result of internal condensation USB cable connection Line power in Figure 1 Rear connections on the Lambda Bio XLS instrument The ON OFF switch is located at the top left hand of the keypad panel 18 Lambda XLS XLS User s Guide Using your Instrument 20 Lambda XLS XLS User s Guide Overview of the Instrument The Lambda XLS XLS instrument is a simple to use UV Visible spectrometer with a CCD array detector 1024 pixels To access the folders and functions on your Lambda XLS XLS instrument navigate around the display using the alphanumeric keys and the navigation arrows on the keypad Display panel ON OFF View Options Alphanumeric keys Arrow keys Cancel Back Stop Run OK Next Scan 0A 100 T scan reference Figure 2 Keypad and display Key Action Display panel Displays folders menu options that guide you through taking measurements and viewing your results Q9 Turns the instrument on off Use the four arrows to navigate around the display and select the required setting from the active highlighted option The Options key displays the options for that method See Options on page 25 for more information Options unique to a specific method are described in the relevant section The OA 100 T key sets a reference solution measurement to 0 000 A or 100 T at the current wavelength When in scan mode it performs a reference scan The Can
28. formation 78 Lambda XLS XLS User s Guide Maintenance 80 Lambda XLS XLS User s Guide General Maintenance Unauthorized Adjustments and Servicing Do not attempt to make adjustments replacements or repairs to this instrument There are no user serviceable parts in this instrument If you have any problems access the Troubleshooting Guide on the PerkinElmer website and work through the process there WARNING http www perkinelmer com If you do not have access the Troubleshooting Guide or have any further problems contact Customer Care see Appendix 2 Customer Care on page 85 for more information Before using any cleaning or decontamination methods except those CAUTION specified here users should check with Customer Care that the proposed method will not damage the equipment Cleaning the instrument External Cleaning Switch off the instrument and disconnect the power cord before cleaning your instrument Clean the outside of the instrument using a damp cloth A mild liquid detergent may be used if necessary Always perform a patch test on an inconspicuous area of the instrument before you clean the entire instrument Clean all spilled materials from the affected area immediately and wipe it dry with lint free paper or cloth Removing the cell holder for cleaning The sample cell holder can be removed for cleaning 1 Loosen the thumbscrews on the sample holder cover on the base of the instrument by
29. g the keypad numbers or left and right arrows SD Memory Card 1 2 Open the Methods folder Press NE to display the available Options 28 Lambda XLS XLS User s Guide 3 Press 4 to select SD Memory Card The SD Memory Card screen is displayed SD Memory Card Operation 4 Backup Folder d Folder Favourites e OK B Cancel 4 Select the mode of Operation using the left and right arrows then press the down arrow The options are Backup Folder if you wish to copy the contents of the folder to the SD card and Restore Folder if you wish to restore the contents of the folder to the content of the SD card Backup All Folders copies the contents of all the Methods folders to the SD card Restore All Folders restores the contents of all the Methods folders to the content of the SD card NOTE The LED next to the card will be lit while methods are saved to the card The card must not be removed while the LED is lit as it will corrupt your method s 5 Select the Folder using the left and right arrows 6 Press OK mn Method Options Press to display the available Options in the Methods folder which you can select using the keypad numbers Text Function Delete Method Deletes the Method Lock Method Allows the user to Lock the method Unlock Method Allows the user to Unlock the method Using your Instrument 29 Delete Method 1 Open the
30. g yl pmol ul mg dl mmol l pmol l g l mg l ug l U I 9o ppm ppb conc or none The Units screen also enables you to select the number of displayed decimal points DP The options are Auto 0 1 and 2 NOTE The result will always be fixed to 5 significant figures regardless of how many decimal points are selected so 98768 2 is displayed as 98768 even with 1 decimal point selected Press OK D to save your options and return to the Method Settings screen The units can still be edited 6 Select the type of Curve Fit using the left and right arrows then press the down arrow The options are Zero Regression this forces the straight line through the origin Regression straight line regression Interpolation linear interpolation or Cubic Spline 7 Select the Calibration mode using the left and right arrows The options are Standards to measure prepared standards or Manual entering absorbance values by keypad or New Standards to replace any standards that have already been collected 8 If you selected Standards press the down arrow and enter the number of Replicates This determines the number of standards to be measured and averaged at each standard concentration point The options are Off 1 2 or 3 NOTE If you selected Manual in Step 7 you do not enter Replicates 56 Lambda XLS XLS User s Guide 9 Press Next gt The Standard Curve Standards screen is displayed
31. h a sample relative to a reference this can be air The procedure is as follows 1 Press 1 to open the Standard Methods folder The Standard Methods screen is displayed Standard Methods ofA forse Wavelength O O ene A s A i e X Multi wavelength e Absorbance Ratio om Spectrum Q NS Concentration 9 A Standard Curve 2 Press 1 to open the Single Wavelength method The Single Wavelength Method Settings screen is displayed Single Wavelength Method Settings Wavelength Absorbance B Cancel 3 Set the Wavelength by using the keypad numbers or the left and right arrows then press the down arrow 4 Select the Mode using the left and right arrows The options are Absorbance or Transmission Instrument Methods 39 5 Press OK gt to start measurements The Single Wavelength results screen is displayed Single Wavelength Sample 620 nm Result 0A 0AT 6 Insert the reference sample and press oa This reference measurement will be used for all subsequent samples until repeated 7 Insert your first sample and press mn The result at the selected wavelength is displayed on screen Use the left and right arrows to move the cursor and display the value at the cursor position 420 nm from the set wavelength Single Wavelength Sample 1 A 620 nm Result M 0 038 Units A 8 Repea
32. he Utilities folder The Utilities screen is displayed Utilities e 163 pate and Time Q i 1 p About e F8 reser a Ea Games T eis Q 1 Preferences e Oor Figure 9 The Utilities screen The Utilities are Date and Time Set correct time and date Regional Select preferred language and number format Printer Printer output options Preferences Select screen layout themes and history Contrast Adjust screen contrast and brightness About Serial number and software version Games Spectro Blocks Sudoku 32 Lambda XLS XLS User s Guide Date and Time 1 Press 1 to select Date and Time The Date and Time screen is displayed Date and Time January 33 2008 B Cancel 2 Using the keypad numbers or left and right arrows enter the Day Month Year Hour and Minute 3 Press OK to store the settings to store the settings and return to the Utilities folder Seconds are zeroed when you press OK Regional 1 Press 2 to select Regional The Regional screen is displayed Regional Language mm Number Format 999 9 B Cancel 2 Using the left and right arrows select a Language and then press the down arrow The options are English French German Italian Japanese or Spanish Using your Instrument 33 3 Select the Number Format the type of decimal separator using the left and right arrows The options are
33. ignificant information that is provided with some procedures We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons Details about these circumstances are in a box like this one WARNING We use the term CAUTION to inform you about situations that could result CAUTION in serious damage to the instrument or other equipment Details about these circumstances are in a box like this one 8 Lambda XLS XLS User s Guide Safety Information 10 Lambda XLS XLS User s Guide General Safety The Lambda Bio XLS instruments conform to the requirements of the Low Voltage Directive 2006 95 EC EMC Directive 2004 108 EC and the IVD Directive 98 79 EC Standards to which conformity is declared e EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use e EN 61010 2 101 Particular requirements for IVD medical equipment e BS EN 591 Instruction for use for in vitro diagnostic instruments for professional use e BS EN 13612 Performance evaluation of in vitro diagnostic medical devices e EN 61326 2 3 Electromagnetic compatibility generic emission standard Electrical equipment for measurement control and laboratory use e EN 61326 A1 A2 A3 Electromagnetic compatibility generic emission standard Electrical equipment for measurement control and laboratory use Classified as Class B for conducted and radiat
34. in as the Printer option using the arrow keys Printer Auto Print Printer 4 Press OK to save the settings Accessories 73 Loading or changing the printer paper 1 Lift off the paper compartment cover Platen lock released 2 Lock the platen and turn the knob to feed the paper Platen locked 3 Feed in the paper Sometimes it helps if the platen lock is released 4 Replace the cover 7 4 Lambda XLS XLS User s Guide Bluetooth Accessory Installation 1 Remove the power cable from the instrument 2 Turn the instrument over and remove the two cap head using the 3 mm hexagonal wrench provided 3 Turn the instrument over and remove the two cap head screws using the 3 mm hexagonal wrench provided Cap head screws 4 Turn the instrument back over and lift the accessory cover vertically upwards to remove it 5 Remove the tie wrap from the cable 6 Plug the accessory cable into the Bluetooth module Accessories 75 7 Plug the two lugs on the Bluetooth module into the innermost slots in the base of the case gt di 8 Lower the accessory cover vertically downwards onto the instrument engaging the PCB in the innermost slots in the accessory cover Bluetooth accessory slots 9 Invert the instrument and replace the two cap head screws 10 Connect the power cable 11 Switch the instrument on and open the Utilities folder 76 Lambda XLS XLS User s Guide
35. ing the keypad numbers press the down arrow and then go to Step 12 The available range is from 1 00 to 9999 OR If the dilution factor is unknown press Options to display the Dilution Factor screen Dilution Factor Enter the Volume of the sample using the keypad numbers then press the down arrow The available range is from 0 01 to 9999 11 12 13 14 15 16 17 Instrument Methods 67 Enter the volume of Diluent by using the keypad numbers The available range is from 0 01 to 9999 Press OK to calculate the dilution factor and return to the Method Settings screen Select the Units of measurement using the left and right arrows then press the down arrow The options are pg ml ng ul ug pl Enter the Factor using the keypad numbers then press the down arrow The available range is from 0 001 to 9999 Press OK to start measurements The Absorbance Ratio results screen is displayed Absorbance Ratio Sample A260 A280 Concentration uml 0A ord Insert the reference sample then press MW This reference measurement will be used for all subsequent samples until repeated Insert your first sample and press mn The absorbance at the selected wavelengths is measured corrected by the background wavelength value if this was selected and the ratio between Wavelength 1 and Wavelength 2 is calculated 68 Lambda XLS XLS U
36. ivates the start and finish points of the desired graph to user defined specific wavelengths and or absorbance values gt Press EI to cancel the selections press to accept them and display the required graph Sample Number Adds a prefix to the sample number and resets the incrementing number to the desired value Save Method Use the left and right arrows to select a folder to store the method in Favorites Methods 1 9 then press the down arrow and enter a name for the method Press Save to save the method Auto print Toggles Auto Print on and off gt Press or O or wait 20 seconds to exit the method options gt Press EI to return to the Standard Methods folder after all your samples have been measured Instrument Methods 49 Concentration The Concentration method makes simple concentration measurements on samples by measuring the amount of light that has passed through a sample relative to a reference this can be air The concentration is determined by measuring the absorbance at a specific wavelength and then multiplying by a factor If the factor is known in advance it can be entered into the method If the factor is not known a standard of known concentration can be measured and the factor then calculated by the instrument The procedure is as follows 1 Press 1 to open the Standard Methods folder The Standard Methods screen is displayed Standard Methods O A orate waveenan
37. l purpose nature of these instruments and the wide variety of uses to which it can be put it is not possible to define exact decontamination procedures for the many types of samples being tested These should be established by the user or their Health and Safety representative before use of the instrument Guidance can be found in document M29 A3 Protection of Laboratory Workers from Occupationally Acquired Infections Approved Guidelines published by the US Clinical and Laboratory Standards Institute The sample compartment may be removed and cleaned by soaking in an appropriate disinfectant to the bio hazard that has occurred for at least 30 minutes The compartment should then be rinsed in distilled water and allowed to ary Similarly the unit exterior can be wiped over with an appropriate disinfectant with a wipe or soft cloth Any internal spills should be wiped up with absorbent material through the sample compartment aperture and similarly decontaminated using disinfectant soaked wipes Decontamination Certificates If the instrument needs to be returned a decontamination certificate is required Decontamination certificates can be found at the following web address or be provided by Customer Care http www perkinelmer com It is the responsibility of the user to ensure a safe working environment is provided to the engineer or staff by appropriate risk assessments and if appropriate decontamination procedures before work commence
38. n of the sample is displayed Concentration Wavelength Sample 261 nm 7 Absorbance Concentration 0 n 0 0 Factor e 50 0 12 Repeat Step 11 for each sample Instrument Methods 53 gt Press to display the available Options which you can select using the keypad numbers Method Settings Returns to the Method Settings screen Print Prints result via selected method Show Graph Toggles the graph on and off The wavelength range of the graph displayed is 20 nm from the selected wavelength which is marked by the cursor Run Standard Returns to the Run Standard screen Sample Number Adds a prefix to the sample number and resets the incrementing number to the desired value Save Method Use the left and right arrows to select a folder to store the method in Favorites Methods 1 9 then press the down arrow and enter a name for the method Press Save to save the method Auto Print Toggles Auto Print on off Press or O or wait 20 seconds to exit the method options v Press EI to return to the Standard Methods folder after all your samples have been measured 54 Lambda XLS XLS User s Guide Standard Curve This method enables the construction of a multi point calibration curve from standards of known concentration that can then be used to quantify unknown samples This instrument has the advantage of being able to store
39. neral Maintenance ees se ee Re ee enne nennen RR ee Ee EE RR nnn nn nnn nn Re 80 Cleaning the instrument esse ee aa a RR ER ee ee ee ee ee Ee Ee 80 Storage and SHIPPING siese est ener tn irn FEE NR cies nenne ra radi 82 APPCNICeS mM 83 Appendix 1 Specifications seeseeeeeeeee nnne nmn nnn 84 Introduction 6 Lambda XLS XLS User s Guide About this Manual This user s guide describes the installation and operating procedures for your Lambda XLS XLS UV Vis spectrometer The manual contains the following sections Introduction Safety Information Installing your Instrument Using your Instrument Instrument Methods Accessories Maintenance Appendices Introduction 7 Conventions Used in this Manual Normal text is used to provide information and instructions Bold text refers to text that is displayed on the screen UPPERCASE text for example ENTER or ALT refers to keys on the PC keyboard The symbol is used to show that you have to press two keys at the same time for example ALT F All eight digit numbers are PerkinElmer part numbers unless stated otherwise Unless stated otherwise in this manual instrument refers to the Lambda XLS or Lambda XLS instrument Notes cautions and warnings Three terms in the following standard formats are also used to highlight special circumstances and warnings NOTE A note indicates additional s
40. ngs 2 screen is displayed 62 Lambda XLS XLS User s Guide 8 Kinetics Method Settings 2 4 DeltaA gt Select the measurement Mode using the left and right arrows then press the down arrow The options are Delta A the change in absorbance over the measurement duration or selected period Final A the absorbance at the end of the measurement duration or selected time and Slope the rate of change of absorbance over the measurement duration or selected period Enter the Units then press the down arrow You can enter a text string up to 8 characters long To access a list of pre defined units on the Units screen press Options and then use the left right arrows pg ml H9 ul pmol ul mg dl mmol l pmol l g l mg l ug l U I ppm ppb conc or none These units can also be edited once OK is pressed The Units screen also enables you to select the number of displayed decimal points DP The options are Auto 0 1 and 2 NOTE The result will always be fixed to 5 significant figures regardless of how many decimal points are selected so 98768 2 is displayed as 98768 even with 1 decimal point selected 10 Press OK to save your options and return to the Method Settings screen The units can still be edited Set the Factor by which the result is multiplied to give the amount in the chosen range using the left and right arrows The available range is from 0 01
41. notice Specification Lambda XLS Lambda XLS Wavelength range 190 1100 nm Wavelength scanning range 200 950 nm Monochromator Flat grating Wavelength calibration Automatic when the instrument is switched on Spectral bandwidth 5 nm 3 nm Wavelength accuracy 42 nm Wavelength reproducibility 1 nm Light sources Pulsed xenon lamp Detector 1024 element CCD array Absorbance range 0 300 to 2 500 A 0 to 199 T Photometric linearity 40 005 A or 1 of the reading whichever is the greater at 546 nm Photometric reproducibility 0 003 A 0 0 5 A 0 007 A 0 5 1 0 A Stray light 0 596 at 220 nm and 340 nm using NaNO Zero stability 0 01 A hour after 20 minutes warm up at 340 nm Noise 0 005 peak to peak 0 002 rms Digital output USB port standard Bluetooth option Dimensions 340 x 310 x 170 mm 340 x 420 x 170 mm with printer attached Weight Approximately 4 5 kg Power input 100 250 V 50 60 Hz Max 30 VA Appendices 85 Appendix 2 Customer Care Contacts NOTE If you experience any problems with your instrument please see the Troubleshooting Guide at http www perkinelmer com before contacting Customer Care Customer Care USA 1 00 762 4000 In the USA 1 203 925 4602 Outside USA Customer Care EU 0800 40 858 Belgium 0805 111 333 F
42. ntroduction 1eeee eese RR napaka Aara KEER KEE REK EER NKR EE REK EE ERK PRAA ERES 5 About this Manual is ie DEE EE AE ES EN eee serere Dea va veo c veru TER EE EXE CX RR See eeue Ee 6 Conventions Used in this Manual sees ss ee ee ee EE ee RE Re nnns 7 Notes cautions and warnings eise ee ee ee ER EA ee RR EE ee de ee ee 7 Safety Information issie e RR KERE KEER RR EE REK EER nennen nnne nnnm ERK EE nnn ERK 9 General Safety EO EE EE EEN 10 Decontamination Certificates es ese ee Ee ee AE ee AE ee Re Ee ee 11 ede RT EE NE AE EE AE 11 TOXIC SS OR OE Ed oe ee Ye Lee XO EE Mis 11 Waste Disposal ES 11 Personal Protective EguipmeNE ee ee ee ee Ee ee ee ee ee ee ee ee 11 Intended User is ER ties ER EE ee Ee ee tre et e aaa 12 Unpacking and Installation ee ee ee ee ee RR EE ee ee ee RR ER een 13 Installing your Instrument sesse sees kk RE EER KERE nnne enne nnn 15 UNPACKING EE 16 Connecting to the Line Power Supply eeeeeennn mnn 17 Using your Instrument esse ee saak EER sienne enne 19 Overview of the InstrumeNE ees ese ee ER ee ee nennen nennen nnns 20 Switching on the Instrument ees ee ee ee ee RE Ee ee ee ER Ee ee ed eke ee 22 Usirig the SoftWale iiec iex enr eda ter ina RARR tarsi PR EES EN Dal Ee Rise ie 23 Methods and Favorites iisto irasai nennen nnn nnn nnns 26 Folder Optiofis RE EE eie oe cce Pe EE EOE ER 26 Method Options 2 trem PI
43. own arrow Instrument Methods 45 5 Select the Mode using the left and right arrows then press the down arrow The options are Absorbance or Transmission 6 Press OK to start measurements The Spectrum results screen is displayed 0 0 400 420 440 460 480 500 A A Sample A 450nm 0A OAS 7 Insert the Reference sample and press E This reference measurement will be used for all subsequent samples until repeated 8 Insert your first sample and press mn A spectrum and a table of the Absorbance 9o Transmission values at each peak are displayed Use the left and right arrows to move the cursor along the graph When it reaches a peak the peak height and width are displayed at the top of the screen To zoom in on the wavelength scale use the up arrow key This automatically scales the Absorbance Transmission axis depending upon the Graph Scale option and this is retained for subsequent measurements To zoom out again use the down arrow key Height 0 4654 width 13 0nm 400 450 500 550 600 650 700 A 434 521 626 A 0 1 s7 0 462 0 558 Sample 1 A 521nm A 0 462 4 9 Repeat Step 8 for each sample 46 Lambda XLS XLS User s Guide gt Press to display the available Options which you can select using the keypad numbers Method Settings Returns to the Method Settings screen Print Prints re
44. rance 0800 0 00 66 79 Germany 0800 90 66 42 Italy 800 099 164 Spain 0800 896 046 UK Customer Care Brazil 11 38 68 6200 Customer Care Canada 800 561 4646 Customer Care China 10 5820 8166 Customer Care India 022 6760 1700 Customer Care Japan 45 339 5889 Customer Care Singapore 67799 539 66 Lambda XLS XLS User s Guide Index A Absorbance Ratio enne 65 ACCESSONIES EE ee ccce vec ri vo 70 Add Peak sisi e oet eres Ee 47 B Bluetooth Installation 74 C Cleaning the instrument 80 ConcentratiON ees ese ee ee RE EE RR EE 49 Consumables enn 70 Bo nus cu BED N 34 Conventions keyboard text seen 7 Notes cautions and warnings 7 on screen LOX ses EE ENE ERK EE He EE EE ERK E EE 7 io OER ee aad YR 7 Customer Care ese ee ee ee RE Ee n 85 D Bel EE N eee 20 F Favorites o dd voe deer GE Ge Gee Es 26 G Graph Scale ees ee Re ee EE RE Re EE 48 I Installation Connecting to the Power Supply 17 Unpacking eene 16 Instrument Home Page 22 Instrument Overview s 20 K 237 T 20 IAlnzi e cn ee cess AE coweses ta 60 L Latiguage iei 32 M Maintenance enn 80 Methods EE et 26 Absorbance Ratio seeen 65 Concentra
45. raph on and off The cursors display the absorbance and corresponding concentration values of the last measured sample Sample Number Adds a prefix to the sample number and resets the incrementing number to the desired value Save Method Use the left and right arrows to select a folder to store the method in Favorites Methods 1 9 then press the down arrow and enter a name for the method Press Save to save the method Auto print Toggles Auto Print on and off gt Press Y or O or wait 20 seconds to exit the method options gt Press El to return to the Standard Methods folder after all your samples have been measured 60 Lambda XLS XLS User s Guide Kinetics Kinetics studies where the change in absorbance needs to be followed as a function of time at a fixed wavelength can readily be performed using the Kinetics method Reagent test kits are routinely used for the enzymatic determination of compounds in food beverage and clinical laboratories by measuring NAD NADH conversion at 340 nm The change in absorbance over a specified time period can be used to provide useful information when an appropriate factor defined in the reagent kit protocol is applied Reaction rate and enzyme activity can be calculated if the factor used takes account of the absorbance difference per unit time as opposed to only the absorbance difference For this reason the change in absorbance per minute AA min concentration
46. s Chemicals Use store and dispose of chemicals that you require for your analyses in accordance with the manufacturer s recommendations and local safety regulations Toxic Fumes If you are working with volatile solvents or toxic substances then you must provide an efficient laboratory ventilation system to remove vapors that may be produced when you are performing analyses Waste Disposal Waste containers may contain corrosive or organic solutions and small amounts of the substances that were analyzed If these materials are toxic you may have to treat the collected effluent as hazardous waste Refer to your local safety regulations for proper disposal procedures Personal Protective Equipment No Personal Protective Equipment PPE is required for operation of the equipment although the samples under test may require the use of such PPE and should be covered by a risk assessment made by the user or the appropriative Health and Safety representative 12 Lambda XLS XLS User s Guide Intended User This instrument is intended for use by individuals trained in and familiar with the use of spectrophotometers and their associated hazards as detailed above In the event of a malfunction or hazard occurring the user responsible shall disconnect the unit from power and isolate the instrument for decontamination by an appropriate means if biohazardous material is spilled on in or around the instrument Safety Information 13
47. s folder Folder Options Press XJ to display the available Options in the Methods folder which you can select using the keypad numbers Text Function Folder Names Allows the Method Folder to be renamed Lock Folder Allows the user to Lock the folder Unlock Folder Allows the user to Unlock the folder SD Memory Allows the User to open the SD card This option is only Card applicable if you have an SD installed in the instrument Using your Instrument 27 Folder Names 1 6 Open the Methods folder Press to display the available Options Press 1 to select Folder Names Select the Folder you wish to rename using the left and right arrows and then press the down arrow Enter a New Name for the folder using the alphanumeric keypad Press OK to rename the folder Lock Folder 1 6 Open the Methods folder Press to display the available Options Press 2 to select Lock Folder Select the Folder you wish to lock using the left and right arrows and then press the down arrow Enter a Pass Code for the folder using the keypad numbers or left and right arrows Press OK to lock the folder Unlock Folder 1 5 Open the Methods folder Press B to display the available Options Press 3 to select Unlock Folder Select the Folder you wish to unlock using the left and right arrows and then press the down arrow Enter the Pass Code for the folder usin
48. ser s Guide Absorbance Ratio 260 nm Sample 0 008 A 1 280 nm A gp A an 1 000 Concentration 0 008 pg ml 18 Repeat Step 17 for each sample gt Press Ey to display the available method options which you can select using the keypad numbers Method Settings Returns to the Method Settings screen Print Prints via selected method Show Graph Toggles the graph on and off The graph shows a spectrum across the range of the measured wavelengths which are marked by cursors Sample Number Adds a prefix to the sample number and resets the incrementing number to the desired value Save Method Use the left and right arrows to select a folder to store the method in Favorites Methods 1 9 then press the down arrow and enter a name for the method Press Save to save the method Auto Print Toggles Auto Print on and off gt Press or O or wait 20 seconds to exit the method options gt Press EI to return to the Standard Methods folder after all your samples have been measured Accessories 70 Lambda XLS XLS User s Guide Accessories and Consumables Supplies accessories and replacement parts can be ordered directly from PerkinElmer See the PerkinElmer website at http www perkinelmer com for more information Part Number Accessory Consumable L7110230 Lambda Bio XLS Printer Module L7110231 Lambda Bio XLS Print Utility and Cable
49. sult via selected method A T Toggles between Absorbance and Transmission mode Peak Detection Displays the Peak Detection settings screen Auto detect Peaks Peak Detect on Zoom Min Peak Height Sort Peaks By 0 00 wavelength Min Peak Width Indicate Peaks No 5 NM p OK B Cancel Auto detect Peaks Turns on and off the automatic peak detection The following options determine how peaks are detected e Min Peak Height Minimum height the peak has to be above the higher of the two adjacent minima for the peak to be detected Min Peak Width Minimum width of the peak as determined by the difference in wavelength between the higher of the two adjacent minima and the opposing intersection of that higher minimum level and the peak profile Peak Detect on Zoom Determines if peaks are reassessed and tabulated when you zoom into a region of the spectrum If No is selected the peak detection remains as determined before zooming Sort Peaks By Determines the sequence that peaks are reported by The options are Peak Width Wavelength and Peak Height Indicate Peaks Switches display of peak cursors on and off The peak cursors show vertical dashed lines displaying the measured peak height and horizontal dashed lines showing the peak width Press m to cancel the selections press gt to accept them Add Peak Delete Peak Instrument Methods 47 Add Peak manually ad
50. t Step 7 for each sample 40 Lambda XLS XLS User s Guide Press to display the available Options which you can select using the keypad numbers Method Settings Returns to the Method Settings screen Print Prints result via selected method A T Toggles between Absorbance and Transmission mode Print Graph Prints Graph this option is grayed out if no data are available Sample Number Adds a prefix to the sample number and resets the incrementing number to the desired value Save Method Use the left and right arrows to select a folder to store the method in Favorites Methods 1 9 then og the down arrow and enter a name for the method Press Save to save the method v Auto Print Toggles Auto Print on and off EIN Press or or wait 20 seconds to exit the method options Press O to return to the Standard Methods folder after all your samples have been measured Instrument Methods 41 Multi Wavelength The Multi Wavelength method can provide absorbance readings at up to 5 wavelengths The procedure is as follows 1 Press 1 to open the Standard Methods folder The Standard Methods screen is displayed Standard Methods o A sees wavelength Q lo Kinetics Ma e AN Multi Wavelength Gp A Absorbance Ratio uu Spectrum S Q XT Concentration Gy res Standard Curve 2 Press 2 to open the Multi Wavelength method The Multi
51. this curve as a method using up to nine standards If you wish to have a zero concentration standard include this in the number of standards to be entered and enter 0 00 for the Concentration then use a reagent blank when required to enter the zero standard The procedure is as follows 1 Press 1 to open the Standard Methods folder The Standard Methods screen is displayed Standard Methods o A forse wavelength Q Kinetics X Multi Wavelength a Absorbance Ratio e 9 lm Spectrum Q gt Concentration o9 d Standard Curve 2 Press 5 to open the Standard Curve method The Standard Curve Method Settings screen is displayed Standard Curve Method Settings ENTE NNCTTIOXNNN 430 Nm Zero Regression 4 Standards wa ml Off Be Next B Cancel 3 Enter the required Wavelength using the keypad numbers or left and right arrows then press the down arrow Instrument Methods 55 4 Enter the number of Standards to be used in the standards curve then press the down arrow The options are from 1 9 5 Enter the Units then press the down arrow You can enter text up to 8 characters long using the keypad or press Options to display the Units screen and access a list of pre defined units Auto NN OK B Cancel In the Units screen use the left and right arrows to select from the available options The options are pg ml u
52. tiON ee 49 io RR AE ras si ie 60 Method folders sesse ees ee ee 26 Method OPDHONS esse ees ee ee 28 Multi Wavelength 41 Single Wavelength sssss 38 Spectrum eene nens 44 Standard Curve sesse ee ee ER RA ee 54 Multi Wavelength ee ese ee ee 41 O ODpLIOFIS T I TET TE TTN 25 P Peak Detection eese 46 Preferences ees ee Ee Re Ee EE RR Re EE 34 Printer Configuration ee ee ee RE RA ee 72 InstallatiON ee sees ee ee RE RA ee 71 Loading Paper sesse RA ee 73 Printer Setup eine pee 33 R Report Utility ees ee RE ee EE 77 Report Viewer es ese ee ee Re RR ee 77 S Safety General SafetY see ee ERA ee 10 Unpacking and Installation 13 Sample Handling 36 SD Memory Card esse ees ee EE 27 SHIPPING aers RR sees ete e even as 82 Single Wavelength 38 ke RE EE 63 SpecificatiONS esse se ee Ee RR Ee EE 84 SpeCLEUITI EE 44 Standard Curve sesesss 54 Standard Methods Absorbance Ratio serere 65 ConcentratiON ee 49 lo EE 60 Multi Wavelength 41 Single Wavelength ssss 38 SPEEEUIMIE Ese EE Se Oe Mee a gek 44 Standard Curve sesse ee ER RA ee 54 Startup and Shutdown 22 Storage edo dece rete eni ark 82 Index 87 Using the
53. to 9999 11 12 13 Instrument Methods 63 Press OK gt to start measurements The Kinetics results screen is displayed 2 5 2 0 1 5 1 0 0 5 0 0 0 0 0 2 0 4 0 6 0 8 1 0 Ap An dA Slope Result R2 0A OA Insert the reference sample and press II This reference measurement will be used for all subsequent samples until repeated Insert your first sample and press to start the run The running Time is displayed at the bottom of the screen and absorbance data are plotted on the graph as the measurement proceed The table below the graph displays Ao the absorbance value at to start of calculation A the absorbance value at t finish of calculation dA the change in absorbance the Slope and the regression parameter R of the calculated slope The concentration is a calculated using the parameters selected in Method Settings 2 dA Final A or Slope the name of the Result table column depends on the units chosen 0 6 0 5 0 4 0 3 0 2 01 0 0 0 2 0 4 0 6 0 8 1 0 Ao An dA Show Slope Result R2 0 004 0 006 0 002 0 402 0 002 0 2290 Sample 1 Time 00 00 30 4 0 059 Use the left and right arrows to move the cursor and display the time and absorbance value at measured data points Use the up and down arrows to zoom in or out 64 Lambda XLS XLS User s Guide gt Press to display the available method
54. uipment must be connected to the supply outlet with the power cord and power supply provided It can be used on 100 240 V 50 60 Hz supplies The power cord or power supply outlet should be is fused at 3 A 250 V e The instrument should be positioned so that the power cable can be readily disconnected from the unit in the event of a hazard or malfunction occurring e Site the instrument in an atmosphere free from dust and corrosive fumes e Please read through the instrument User s Guide supplied on the Lambda Bio XLS Documentation CD L6050018 prior to use e Switch on the instrument via the ON OFF button on the keypad after it has been plugged in The instrument will perform a series of self diagnostic checks If the equipment is used in a manner not specified herein then the protection provided by the equipment may be impaired and instrument warranty withdrawn WARNING 14 Lambda XLS XLS User s Guide Installing your Instrument 16 Lambda XLS XLS User s Guide Unpacking Take great care when installing your instrument and follow the CAUTION procedures described in this manual 1 Check that you have the items listed in the following table Part Number Description L7110189 L7110192 Lambda XLS XLS if applicable L7110190 L7110193 Lambda XLS XLS with printer if applicable L7110191 L7110194 Lambda XLS XLS with SD if applicable L6050014 Lambda Bio XLS Safety Manual L6050018 Lambda Bio
55. unt of light that has passed through a sample relative to a blank this can be air at two wavelengths The procedure is as follows 1 Press 1 to open the Standard Methods folder The Standard Methods screen is displayed Standard Methods E Single Wavelength O ed Kinetics xim X e X Multi wavelength 23 Absorbance Ratio of VV Spectrum S Q I Concentration ej Standard Curve 2 Press 7 to select the Absorbance Ratio method The Absorbance Ratio Wavelengths screen is displayed Absorbance Ratio Wavelengths Wavelength 1 Wavelength 2 280 nm Background Off B Next B Cancel 3 Enter Wavelength 1 by using the keypad numbers or the left and right arrows then press the down arrow 4 Enter Wavelength 2 as above and then press the down arrow 5 Select whether a Background correction is applied to both wavelengths 1 and 2 using the left and right arrows 66 Lambda XLS XLS User s Guide 6 10 If background correction is On enter Wavelength 3 from which the background correction will be obtained Press Next gt The Absorbance Ratio Method Settings screen is displayed Absorbance Ratio Method Settings Pathlength Dilution Factor 1 000 Select the Pathlength using the left and right arrows then press the down arrow The options are 5 mm or 10 mm If the Dilution Factor is known enter a dilution factor by us
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