UV20 User Manual – English
Contents
1. Nature 343 27 Yamada H and Hieda K 1992 Wavelength Dependence 150 290nm of the Formation of the Cyclobutane Dimer and the 6 4 Photoproduct of Thymine Photochemistry and Photobiology 55 541 548 IMPORTANT Request a copy of the Hoefer Inc Health and Safety Declaration form before returning the item No items can be accepted for servicing or return unless this form is properly completed Note A Return Materials Authorization RMA number must be obtained from Hoefer Inc before returning any item to Hoefer Inc Customer service information Technical service and repair Hoefer Inc offers complete technical support for all of our products If you have any questions about how to use this product or would like to arrange to repair it please call or fax your local Hoefer Inc representative Check the Hoefer Inc website at www hoeferinc com for the distributor in your area Or contact us directly at Hoefer Inc 84 October Hill Road Holliston MA 01746 Toll Free 1 800 227 4750 Phone 1 508 893 8999 Fax 1 508 893 0176 E mail support hoeferinc com e pll Ordering information product quantity code number MacroVue UV 20 Transilluminator 20 x 20 cm viewing surface 302 nm 115V 1 UV20 115V 230 V 1 UV20 230V 302 nm UV lamp 8 W 1 UVLM 8 254 nm UV lamp 8 W 1 UVLS 8 UV transmitting work surface 1 UVT WS Fuses for 115 V and 230 V models 5 PSF1A SB 5X20 e pl2 Hoe2. manual MacroVue UV 20 Transilluminator um UV20 IM Rev C0 12 12 A Ato e fe r o Page finder Important Information ii Waste Electrical and Electronic Equipment WEEE ee vii UV transilluminator function 1 Bote IRE 2 SpecificatiONS ticas 2 Important safety information 3 Operating Instru ctlons uuu ice susia s eene i ek 4 Care and maintenance ciues oer res periode temas asma eaa 5 e naL 5 Replacing UV ApS siipiin tette en demais 6 Replacing TUSOS ERR ERE EE LEES 7 Troubleshooting cuis casera c aava eine tune 8 BIDOSTAPY ai i a POR dO 9 Customer service Information 11 Ordering Information 12 Important Information English If this equipment is used in a manner not specified by Hoefer Inc the protection provided by the equipment may be impaired This instrument is designed for indoor laboratory use only Only accessories and parts approved or supplied by Hoefer Inc may be used for operating maintaining and servicing this product Warning Because this instrument can develop suffi cient voltage and current to produce a lethal shock care must be exercised in its operation This instrument is designed in accordance with the EN61010 1 2001 electrical safety standard Never theless it should be used only by properly trained operators Read this entire manual before using the instrument and use only according to the instructions The instrument must always be
3. cifi par Hoefer Inc la protection fourni par l quipement pourrait tre diminu e Cet instrument est concu pour l usage de laboratoire int rieur seulement Seulement les accessoires et les parties ont approuv ou ont fourni par Hoefer Inc pourrait tre utilis pour fonctionner maintenir et entretenir ce produit Avertissant Parce que cet instrument peut d velopper la tension et le courant suffisants pour produire un choc mortel le soin doit tre exerc dans son op ra tion Cet instrument est conform ment concu l EN61010 1 2001 norme de s curit lectrique N anmoins o piii il devrait tre seulement utilis par les op rateurs convenablement entrain s Lire ce manuel entier avant utiliser l instrument et l usage seulement selon les instructions l instrument toujours doit tre utilis avec l avance de terre du cordon d alimentation correctement a fond la terre la sortie principale Utiliser le fil et l quipement lectriques seule ment intacts sp cifiques pour les tensions que vous utiliserez Tout quipement connect haute tension devrait tre conform ment EN61010 1 2001 Garder l instrument aussi sec et propre comme possi ble Essuyer r guli rement avec un doux touffer du tissu Laisser l instrument s che compl tement avant l usage Ne pas fonctionner l instrument dans l extr me humidit au dessus de 8096 Eviter la condensa tion en laiss
4. toto ru n p ed pou it m n stroje a pou it pouze v souladu s pokyny P stroj mus b t v dy pou vaj se na v konu zemi v st ra spr vn zemn ny k zemi na s ti vy stce Vyu it pouze nepo kozen elektrick dr ty a vybaven pro nap t budete pou vat V echna za zen spojen s vysok m nap t m by m la b t v souladu s EN61010 1 2001 Si ponech n stroje jako such a ist jako mo n Ot ete pravideln s a m kk vlhk m had kem Nech je n stroj nenastaven pln p ed pou it m Nejsou provozov na na n stroj v extr mn vlhkost nad 80 P ede lo kondenzaci o pron jmu jednotky na okoln teplotu nech p i p ij m n n stroj z chladn j do tepl ho prost ed Pro umo n n dostate n chlazen zajistit aby otvory n stroje jsou nevztahuje Vigtig Information Danish Hvis dette udstyr bruges i en m de ikke specificeret ved Hoefer Inc den beskyttelse som er blevet forsynet af udstyret kan m ske svaekkes Dette instrument er designet for indendors laboratori umbrug bare Bare tilbehor og del godkendede eller forsynede ved Hoefer Inc kan m ske bruges for drive funktionsfejl og betjening dette produkt Advare Fordi dette instrument kan udvikle tilstraek kelig spaending og strom at fremstille et dodbringende chok skal pleje bruges i dets drift Dette instrument er designet i overensstemmelse med EN61010 1
5. 2001 elektrisk sikkerhedstandard Alligevel skulle det bruges bare af passende traenede operatorer Laes denne hel h ndbog fer bruging instrumentet og brug bare i henhold til instruktionerne Instrumentet skal altid bruges med jordblyet af netledningen rigtigt jordede til jord p hovedledning sudlobet Bruger bare uskadt elektrisk trad og udstyr som v re specifik for spaendingerne du vil bruge Alt udstyr forbundet til hoj spaending skulle vaere i overensstem melse med EN61010 1 2001 Beholder instrumentet s tor og ren som mulig Tor regulaert med et blodt fugtigt stof Lad instrument torken komplet for brug Driver ikke instrumentet i yderst fugtighed ovenfor e pii 8096 Undg kondensation ved lade enheden equili brate til omgivende temperatur ved tageen instru mentets fra et koldere til et varmere miljo Attillade tilstraekkelig afkoling forsikrer at lufthullerne af instrumentet er ikke daekket Belangrijke Informatie Dutch Indien deze uitrusting in een manier wordt gebruikt die niet door Hoefer is gespecificeerd Nv de bescherming die door de uitrusting is verzorgd kan worden geschaad Ditinstrument is voor binnenlaboratoriumgebruik enkel ontworpen Enkel onderdelen en delen keurden goed of leverden door Hoefer Nv kan voor het bedienen worden gebruikt handhavend en onderhouden van dit prod uct Waarschuwend Omdat dit instrument voldoende spanning en stroom kan ontwikkele
6. Importantes Portuguese Seeste equipamento usado numa maneira n o espe cificada por Hoefer Inc que a protecc o fornecida pelo equipamento pode ser comprometida Este instrumento projectado para uso de interior de laborat rio s S acess rios e partes aprovaram ou forneceu por Hoefer Inc pode ser usada para operar Manter e servicing este produto Advertindo Porque este instrumento pode desen volver voltagem suficiente e corrente produzir um choque letal cuidado deve ser exercitado em sua Operac o Este instrumento projectado de acordo com o EN61010 1 2001 condi o de seguran a el ctrica N o e pv obstante deve ser usado s por operadores adequada mente treinados Leia este manual inteiro antes de usar o instrumento e use s de acordo com as instrucc es O instrumento sempre deve ser usado com o chumbo de terra do cord o de poder corretamente baseou a terra nos canos sa da principais Usa fio el ctrico s intacto e equipamento espec fico para as voltagens que voc usar Todo equipamento conectado a voltagem alta deve ser de acordo com EN61010 1 2001 Mantem o instrumento t o seco e limpo como poss vel Limpe regularmente com um pano h mido macio Deixe o instrumento secar completamente antes de uso N o opera o instrumento em humidade extrema acima de 80 Evite condensa o deixando o equili brate de unidade a temperatura ambiental quando tomar o instru
7. et dodelig sjokk m bli ovd bekymring i dets drift Dette instrumentet er utformet i samsvar med EN61010 1 2001 elektrisk sikkerhetsstandard Likevel burde bli brukt det bare av skikkelig utdannede oper atgrer Les denne hele h ndboken fer bruking instru mentet og bruken bare gi til instruksjonene Instrumentet m alltid bli brukt med jorden blyet av kraftkabelen som riktig ha blitt jordet til jord p hovedledningen utlop Bruker bare uskadd elektrisk ledningsfremforing og utstyr som er spesifikk for spenningene du vil bruke All utstyr koplet til hoyspenning burde vaere i samsvar med EN61010 1 2001 Beholder instrumentet som torker og rengjor som mulig Visk regulaert med et mykt fuktig stoff La instru mentet torker komplett for bruk Driver instrumentet i ekstrem fuktighet ikke ovenfor 8096 Unng kondensasjon ved la enheten equili brate til omgivelsestemperatur ved taen instrumentets fra et kaldere til et varmere miljo Til tillate tilstrekkelig kj lig sikrer at ventilasjon s pningene av instrumentet er ikke dekket Wazne Informacje Polish Je eli ten sprz t jest wykorzystywany w spos b nie okre lone przez Hoefer Inc do ochrony przewidzianej przez urz dzenie mo e zosta obni ony Instrument ten jest przeznaczony do u ytku w labora toriach kryty tylko Tylko akcesori w i cz ci zatwierdzone lub dostarczone przez Hoefer Inc mog by wykorzystane do eksp loatacj
8. lle vain Vain lis varusteet ja osat hyv ksyiv t tai toimitti Hoeferin oheen Inc voi k ytt k ytt miselle valvoalle ja servicing t m tuote Varoittaminen Koska t m v line voi kehitt riitt v j nnitteen ja virran tuottaa kuolettavan j rkytyksen huolta t ytyy harjoittaa toiminnossaan T m v line suunnitellaan EN61010 1 2001 s hk turvallisuusstandardin mukaisesti Silti pit isi k yt t vain ohi oikeasti koulutetut k ytt j t Lue t m kokonainen manuaalinen ennen v linett ja k ytt vain ohjeiden mukaan V linett t ytyy k ytt aina valtanuoran maalyijyst perusti oikein maadoittaa s hk verkkoaukossa K ytt vain undamaged s hk metallilankaa ja varus teita t sm llinen j nnitteille k ytt Kaikki varusteet yhdistetty korkeaan j nnitteeseen pit isi olla EN61010 1 2001IN mukaisesti Pit v lineen yht kuiva ja puhdas kuin mahdollinen Pyyhi s nn llisesti pehme ll kostealla kankaalla Anna v line kuivua t ysin ennen k ytt Ei k yt v linett extreme ilmankosteudessa 80 n yl puolella V lt tiivistymist antamalla yksik n equili brate ymp r iv n l mp tilaan kun ottaminen v line kylmempi l mpim mp n ymp rist n Sallia riitt v n j hdytt minen varmistaa ett v lineen ilmarei t peitet n Information Importante French Si cet quipement est utilis dans une mani re pas sp
9. mmelse med EN61010 1 2001 H ller instrumentet d torkar och reng r som m jlig Torka regelbundet med en mjuk fuktig trasa L t instrumentet torka fullst ndigt f re anv ndningen Fungerar inte instrumentet i extrem fuktighet ver 80 Undvik kondensering vid l ta enheten equili brate till omgivande temperatur n r ta instrumentet fr n en kallare till en varmare milj Att till ta tillr cklig kyla ser till att halen av instru mentet inte t cks e pvi English EEE French R German R Ea Italian R Esa Spanish D Swedish R Waste Electrical and Electronic Eguipment WEEE This symbol indicates that the waste of electrical and electronic eguipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your eguipment Ce symbole indique que les d chets relatifs l quipement lectrique et lectronique ne doivent pas tre jet s comme les ordures m nag res non tri es et doivent tre collect s s par ment Contactez un repr sentant agr du fabricant pour obtenir des informations sur la mise au rebut de votre quipement Dieses Symbol kennzeichnet elektrische und elektronische Ger te die nicht mit dem gew hnlichen unsortierten Hausm ll entsorgt werden d rfen sondern separat behandelt werden m ssen Bitte nehmen Sie Kon
10. questo manuale intero prima di usare lo strumento e l uso solo secondo le istruzioni Lo strumento deve essere sempre usato col piombo di terra della spina di alimentazione correttamente hanno messo a terra alla terra alla presa di corrente principale Usa il filo metallico e l apparecchiatura solo intatti elettrici specifici per i voltaggi che lei usera Tutta l apparecchiatura collegata all alto voltaggio dovrebbe essere conformemente a EN61010 1 2001 Tiene lo strumento come secco e pulito come possibile Pulire regolarmente con un morbido per spegnere il panno Lasciare lo strumento asciuga completamente prima dell uso Non opera lo strumento nell umidit estrema al di sopra di 8096 Evitare la condensazione lasciando e piv l unit equilibra alla temperatura ambiente quando portare lo strumento da un pi freddo a un ambiente pi caldo Di permettere raffreddare sufficiente assicura che gli sbocchi dello strumento non sono coperti Viktig Informasjon Norwegian Hvis dette utstyret blir brukt i en m te ikke spesifisert ved Hoefer Inc beskyttelsen som ha blitt git av utsty ret kan bli svekket Dette instrumentet er utformet for innendors labora toriumbruk bare Bare tilbehor og deler godkjente eller forsynte ved Hoefer Inc kan bli brukt for drive vedlikeholde og betjene dette produktet Varsler Fordi dette instrumentet kan utvikle tilstrek kelig spenning og stram til produsere
11. used with the earth lead of the power cord correctly grounded to earth at the mains outlet Use only undamaged electrical wire and equipment specific for the voltages you will use All equipment connected to high voltage should be in accordance with EN61010 1 2001 Keep the instrument as dry and clean as possible Wipe regularly with a soft damp cloth Let the instru ment dry completely before use Do not operate the instrument in extreme humidity above 8096 Avoid condensation by letting the unit equilibrate to ambient temperature when taking the instrument from a colder to a warmer environment To permit sufficient cooling ensure that the vents of the instrument are not covered Dulezit Informace Czech Pokud by toto za zen je pou ito zp sobem kter neni podle Hoefer ochrana poskytovan na z klad Inc za zen m e b t naru ena Tento n stroj je ur en pro vnit n pou it v laborato i pouze Pouze p slu enstv a sti schv len nebo poskyt nut ch Hoefer Inc mohou b t pou ity pro provoz dr bu a dr b tohoto v robku Pozor Proto e tento n stroj m e vyvinout dostate n nap t a proud kter m vyr b t a smrtiacej ok p e mus b t vykon v na v jeho provoz Tento n stroj je ur en v souladu s EN61010 1 2001 elektrick bezpe nostn normy P esto e by m ly b t pou ity pouze dn vy kolen oper tor st cel
12. ant l equilibrate d unit la temp rature ambiante en prenant l instrument d un plus froid un environnement plus chaud Permettre le refroidissement suffisant garantir que les conduits de l instrument ne sont pas couverts Wichtige Informationen German Wenn diese Ausr stung gewisserma en nicht angege ben durch Hoefer Inc verwendet wird kann der durch die Ausr stung zur Verf gung gestellte Schutz verschlechtert werden Dieses Instrument wird f r den Innenlaborgebrauch nur daf r entworfen Nur Zus tze und Teile genehmigten oder lieferten durch Hoefer Inc kann f r das Funktionieren das Aufrechterhalten und die Wartung dieses Produktes verwendet werden Die Warnung Weil dieses Instrument gen gend Strom spannung und Strom entwickeln kann um einen t dli chen Sto zu erzeugen muss Sorge in seiner Operation ausge bt werden Dieses Instrument wird in bereinstimmung mit dem EN61010 1 2001 elektrischen Sicherheitsstandard daf r entworfen Dennoch sollte es nur von richtig erzo genen Maschinenbedienern verwendet werden Lesen Sie dieses komplette Handbuch vor dem Verwenden des Instrumentes und verwenden Sie nur gemaB den Instruktionen Das Instrument muss immer mit der Erdleitung der Macht Schnur richtig niedergelegt zur Erde am Hauptausgang verwendet werden Nur unbesch digte elektrische Leitung und Ausr s tung spezifisch f r die Stromspannungen verwenden die Sie verwenden werde
13. ce after each use Use a mild detergent and water on a soft cloth or sponge to clean the unit exterior Never use abrasive cleaners solvents or chloroform on any part Replacing UV lamps The transilluminator is originally equipped with 302 nm UV lamps If 254 nm wavelength is required replace all lamps with 254 nm lamps of the specified wattage Visually check that all lamps are illuminated If one or more lamps are burned out it is recommended that all lamps be replaced at the same time to ensure even light intensity To replace each lamp Caution Turn the unit offand disconnect the power cord from the mains power e Remove the filter cover by unscrewing the four retainer screws located on the sides of the cover e If present remove the tape that secures the lamps during shipping Remove each lamp by carefully rotating it V turn and then carefully pulling it upward To insert the new lamp hold it so that the metal pins at the end are vertical Place the lamp in the socket and lightly push it up into place Rotate the tube 4 turn until it locks into place Replace the filter cover and screw in the retainer screws Replacing fuses Fuses protect equipment by disconnecting loads too large for the instrument s circuit design For continued protection only replace fuses with the specified voltage and current ratings Each of two round modules on the left side of the control pane
14. e el instrumento seque completa mente antes de uso Noopera el instrumento en la humedad extrema encima de 8096 Evite condensaci n permitiendo la unidad equilibra a la temperatura ambiente al tomar el instrumento de un m s fr o a un ambiente m s tibio Permitir refrigeraci n suficiente asegure que las aberturas del Viktig Information Swedish om denna utrustning anv nds i ett s tt som inte har specificeras av Hoefer Inc skyddet tillhandah ll vid utrustningen kan skadas Detta instrument formges f r inomhuslaboratorium anv ndning bara Bara medhj lpare och delar godk nde eller levererade vid Hoefer Inc kan anv ndas f r fungera underh lla och servicing denna produkt varna D rf r att detta instrument kan utveckla tillr ck lig sp nning och str m att producera en d dlig st t m ste vas omsorg i dess funktion Detta instrument formges i verensst mmelse med EN61010 1 2001 elektriska s kerheten standarden Icke desto mindre b r det anv ndas bara av riktigt utbildade operat rer L s denna hela handbok f re anv nda instrumentet och anv nd bara enligt under visningarna Instrumentet m ste alltid anv ndas med jorden blyet av kraften repet riktigt grounded till jorden p det huvudutloppet Anv nder bara undamaged elektrisk tr d och utrust ning specifik f r sp nningarna du ska anv nda All utrustning kopplats som till h g sp nning skulle vara i verensst
15. ernal 12 1 x 24 1 x 33 7 cm 4 75 x 9 50 x 13 25 in Safety e All electric components are HYPOT tested to 1500 V The filter is covered by a 3 mm 1 8 thick UV blocking safety shield CE certified EN 61010 1 UL 3101 1 CSA C22 2 1010 1 EN 50082 1 Features The dual intensity switch toggles to a lower UV intensity for preparative work Removable power cord e Lighted power switch Important safety information When working near UV radiation always wear at least a UV blocking face shield and cover all exposed skin For added eye protection wear goggles Make sure all persons in the area are also properly protected Lower the UV blocking safety cover before switching the UV lamps on Always wear protective clothing even with the cover closed to prevent accidental exposure to UV radiation If the cover has been removed you must wear a safety shield and gloves to prevent damage to eyes and skin If possible locate the transilluminator in a controlled environment such as a darkroom Always disconnect the mains power cord before cleaning the unit or replacing fuses or lamps Note To protect the filter from cuts and corrosive buffers cover it with a protective surface such as the Hoefer UV transmitting work surface before placing the sample Note To remove the safety cover for photography lift the lid into the up position and pull out both inserts in the hinge assembly Take extra precautions to
16. fer Inc 84 October Hill Road Holliston MA 01746 Toll Free 1 800 227 4750 Phone 1 508 893 8999 Fax 1 508 893 0176 E mail support amp hoeferinc com Web www hoeferinc com Hoefer is a registered trademark of Hoefer Inc O 2012 Hoefer Inc All rights reserved Printed in the USA Hoefer
17. i utrzymania i obs ugi tego produktu Uwaga Poniewa ten akt prawny mo e by rozwini cie odpowiednich napi cie i bie cych do wyprodu kowania miertelnego szoku opiek musi by wykony wane w dzia aniu Ten instrument zosta zaprojektowany zgodnie z tym EN61010 1 2001 Bezpiecze stwo elektryczne standard Niemniej jednak nale y stosowa jedynie przez odpowiednio przeszkoleni operator w Znajd pa stwo to ca y podr cznika przed zastosowaniem instrumentu i stosowa jedynie zgodnie z instrukcjami Instrument musi zawsze by wykorzystane z ziemi doprowadzi do zasilania detonuj cego w a ciwie uzasadnione na ziemi w sieci wodoci gowej rynku zbytu Wykorzystanie tylko nieuszkodzona elektrycznych drut w i urz dzenia specjalne do napi zap ac wykorzystania Wszystkie urz dzenia pod czone do wysokiego napi cia powinny by zgodne z EN61010 1 2001 Kontrolowa instrumentu jako suche i czyste jak to mo liwe Wytrze regularnie przy pomocy mi kkiego wilgotnej szmatki Niech si instrumentem ca kowicie wysuszy przed u yciem Nie prowadz do instrumentu w skrajnych wilgotno ci powy ej 80 Zapobiec kondensacji najmu przez jednostk r wnowa y do temperatury pokojowej przy podejmowaniu instrumentu z ch odniejsze w cieplejszych rodowiska Aby umo liwi wystarczaj ce ch odzenia zapewniaj ze rozci cia of the instrument nie obj te ubezpieczeniem Informa es
18. l above the mains power cord receptacle holds one T 1A 250V 5 x 20 mm Slow blo fuse Caution Set the power switch to OFF and detach the power cord before replacing fuses e Insert a small flat blade screwdriver into the slot on the fuse module and turn it turn counterclockwise The spring loaded module cap will loosen Pull the cap fuse holder out e Pull the fuse out of its holder and inspect If the fuse element is burned or broken replace the fuse with an identical type If the fuse appears to be intact check it with an ohmmeter o Insert a good fuse into the holder and then insert this assembly back into the unit Seat the module by inserting the screwdriver into the slot pressing gently and turning the cap turn clockwise Troubleshooting Unit does not operate when power is turned on Check that the power cord is plugged into a working electrical outlet Remove and inspect the fuses Check that the lamps are properly installed Lamps flicker excessively Check that the dual intensity switch is in the high position Check for proper line voltage UV filter surface feels warmer than usual Ensure that there is enough space around the unit so that air can freely circulate Bibliography DNA crosslinking Church M C and G Gilbert 1984 Genomic sequencing Proc Natl Acad Sci USA 81 1991 1995 Khandjian E W 1987 Optimized hybridization of DNA blo
19. mento de um mais frio a um ambiente mais quente Permitir esfriar suficiente assegura que as aberturas do instrumento n o s o cobertas Informaci n Importante Spanish Si este equipo es utilizado en una manera no especifi cado por Hoefer S a la protecci n proporcionado por el equipo puede ser da ada Este instrumento es dise ado para el uso interior del laboratorio s lo S lo accesorios y partes aprobaron o suministraron por Hoefer S a puede ser utilizado para operar para mantener y para atender a este producto Advirtiendo Porque este instrumento puede desar rollar voltaje y corriente suficientes para producir un golpe mortal el cuidado debe ser ejercitado en Su operaci n Este instrumento es dise ado de acuerdo con el EN61010 1 2001 est ndar el ctrico de seguridad No obstante debe ser utilizado s lo por operarios adecuadamente capacitados Lea este manual entero antes de utilizar el instrumento y el uso s lo seg n las instrucciones El instrumento siempre debe ser utilizado con el plomo de la tierra del cable de alimentaci n moli correcta mente a la tierra en la salida de red Utiliza alambre y equipo el ctricos s lo ilesos espec ficos para los voltajes que usted utilizar Todo equipo conectado al voltaje alto debe ser de acuerdo con EN61010 1 2001 Mantiene el instrumento tan seco y limpio como posible Enjugue regularmente con un suave el trapo h medo Permita qu
20. n Die ganze mit der Hoch spannung verbundene Ausr stung sollte in berein stimmung mit EN61010 1 2001 sein Das Instrument ebenso trocken halten und reinigen wie m glich Wischen Sie regelm ig mit einem weichen befeuchten Sie Stoff Lassen Sie das Instru ment trocken v llig vor dem Gebrauch Das Instrument in der u ersten Feuchtigkeit ber 80 96 nicht bedienen Vermeiden Sie Kondensation die Einheit equilibrate zur Umgebungstemperatur laBend wenn Sie das Instrument von einem k lteren bis eine w rmere Umgebung nehmen Um das gen gend Abk hlen zu erlauben stellen Sie sicher dass die ffnungen des Instrumentes nicht bedeckt werden Informazioni Importanti Italiano Sequest apparecchiatura usata in un modo specificato da Hoefer Inc la protezione fornito dall apparecchiatura potrebbe essere indebolita Questo strumento disegnato per l uso di laboratorio interno solo Solo gli accessori e le parti hanno approvato o hanno fornito da Hoefer Inc potrebbe essere usato per oper are per mantenere e per revisionare questo prodotto Avvertendo Perch questo strumento pu sviluppare il voltaggio sufficiente e la corrente di produrre una scossa letale la cura deve essere esercitata nella sua operazione Questo strumento disegnato conforme mente all EN61010 1 2001 la norma di sicurezza elettrica Tuttavia dovrebbe essere usato degli opera tori solo correttamente addestrati Leggere
21. n om een dodeli jke schok te produceren moet zorg in zijn operatie worden geoefend Ditinstrument is in overeenstemming met de EN61010 1 2001 elektrische veiligheidsstandaard ontworpen Niettemin zou het enkel door goed getrainde bedieningslieden moeten worden gebruikt Lees dit volledige handboek voor het gebruik het instrument en gebruik enkel volgens de instructies Het instrument moet altijd met de aardeleiding van het stroomsnoer correct grondde naar aarde aan het hoofdafzetgebied worden gebruikt Gebruik enkel onbeschadigde elektrische draad en uitrustings specifiek voor de spanningen u zult gebruiken Alle uitrustingen sloten aan aan hoogspan ning zou in overeenstemming met EN61010 1 2001 moeten zijn Houd het instrument zo droge en schone zoals mogelijk Bij Wis regelmatig met een zacht temper doek Verhuur het instrument droogt volledig voor het gebruik Bedien niet het instrument in extreme vochtigheid bovenstaande 80 Vermijd condensatie door het verhuren van de eenheid in evenwicht brengt naar omgevingstemperatuur wanneer nemen het instru ment van een kouder naar een lievere omgeving Om toe te staan voldoende afkoelen verzeker dat de luchtopeningen van het instrument niet bedekt zijn T rke Tietoa Finnish Jos t t varusteita k ytet n tavassa ei m ritetty Hoeferille Inc suojelu ehk isty varusteille saattaa olla avuton T m v line suunnitellaan sis laboratoriok yt
22. prevent skin and eye exposure if the protective cover is removed Operating instructions 0 Place the unit on a stable and level surface Allow space around the unit for air to circulate freely e Plug the power cord into the receptacle at the back of the unit and into a properly grounded electrical outlet of the proper voltage rating The voltage rating of the unit is indicated on the label above the power cord receptacle Wear goggles a UV blocking face shield gloves and cover all exposed skin o Place the sample on the filter and lower the UV blocking clear protective cover Set the dual intensity switch to high and switch the power to on I The indicator lamp in the ON OFF switch should light and after a momentary flickering the UV lamps should light and be visible beneath the protective cover The high intensity setting is for analytical documentation and the low setting is for preparative work the lower setting reduces photonicking and photobleaching To reduce the UV intensity set the dual intensity switch to low The drop in intensity will diminish the level of sample fluorescence o After viewing the sample turn the unit off and clean according to the instructions in the Care and maintenance section on page 5 Caution Always unplug unit from electrical supply before cleaning or drying the unit Care and maintenance Cleaning Clean the filter surfa
23. table for double stranded nucleic acids but is less sensitive for visualizing single stranded nucleic acids The MacroVue UV 20 filter surface is 20 x 20 cm The light intensity level can be set to either high or low The transilluminator can be converted from 302 nm medium wavelength to 254 nm short wavelength by replacing the UV lamps as described on page 6 Illumination area filter Hinged UV blocking safety cover Je cd Dual intensity switch Mains power high and low switch Note Swirl patterns in the UV filter if present do not interfere with viewing or photo documentation of gels Unpacking Unwrap all packages carefully and compare contents with the packing list making sure all items arrived If any part is missing contact your local Hoefer Inc sales office Inspect all components for damage that may have occurred while the unit was in transit If any part appears damaged contact the carrier immediately Be sure to keep all packing material for damage claims or to use should it become necessary to return the unit Specifications Ultraviolet lamps UV 20 Five 8 W 302 nm UV dual bi pin discharge type tubes Filter The factory installed filter is for 254 nm and 302 nm lamps only Do not use with 365 nm lamps The expected life of the filter is 5000 hours Input fuses e 115 V T 1A 250V 5 x 20 mm 2 fuses e 230 V T 1A 250V 5 x 20 mm 2 fuses Dimensions h x d x w Ext
24. takt mit einem autorisierten Beauftragten des Herstellers auf um Informationen hinsichtlich der Entsorgung Ihres Ger tes zu erhalten Questo simbolo indica che i rifiuti derivanti da apparecchiature elettriche ed elettroniche non devono essere smaltiti come rifiuti municipali indifferenziati e devono invece essere raccolti separatamente Per informazioni relative alle modalit di smantellamento delle apparecchiature fuori uso contattare un rappresentante autorizzato del fabbricante Este s mbolo indica que el equipo el ctrico y electr nico no debe tirarse con los desechos dom sticos y debe tratarse por separado Contacte con el representante local del fabricante para obtener m s informaci n sobre la forma de desechar el equipo Denna symbol anger att elektriska och elektroniska utrustningar inte f r avyttras som osorterat hush llsavfall och m ste samlas in separat Var god kontakta en auktoriserad tillverkarrepresentant f r information ang ende avyttring av utrustningen e pvii UV transilluminator Fig 1 MacroVue UV 20 Transilluminator main components function The Hoefer MacroVue Dual intensity UV transilluminator provides a uniform and intense source of 302 nm ultraviolet light to back illuminate transparent fluorescent materials Nucleic acids stained with fluorescent dyes such as ethidium bromide and acridine orange can be detected under UV illumination This visualization method is especially sui
25. tted and fixed to nitrocellulose and nylon membranes BioTechnology 5 165 167 Khandjian E W 1986 UV crosslinking of RNA to nylon membrane enhances hybridization signals Molec Biol Report 11 107 115 Reed K C and D A Mann 1985 Rapid transfer of DNA from agarose gels to nylon membranes Nuc Acid Res 13 7207 7221 DNA nicking Vollrath D and R W Davis 1987 Resolution of DNA molecules greater than 5 megabases by contour clamped homogeneous electric fields Nuc Acid Res 15 7865 7876 Rapid restriction site mapping Whittaker P A and E M Southern 1986 Ultraviolet irradiation of DNA A way of generating partial digests for rapid restriction site mapping Gene 41 129 134 e plo Testing RecA function Maniatis T 1989 Maintenance and Testing of Lysogens of Bacteriophage Lambda in Molecular Cloning A Laboratory Manual eds Sambrook Fritsch and Maniatis 2 96 Ultraviolet sterilization Reich R R and Anderson H D 1986 Sterilization of Membrane Filters with Ultraviolet Radiation Pharmaceutical Manufacturing Jan Elimination of PCR contamination Wilson K H 1992 UV Absorption Complicates PCR Decontamination BioTechniques 13 208 210 Sarkar Gobinda and Sommer Steve S 1991 Parameters Affecting Susceptibility of PCR Contamination to UV Inactivation BioTechniques 10 590 594 Sarkar Gobinda and Sommer Steve S 1990 Shedding Light on PCR Contamination
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