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1. 200 assert failed Firmware error 201 bad error number Firmware error 202 receive queue overflow Caused by external device sending too much data over serial port and ignoring flow control 203 serial port parity error Parity bit error detected with incoming serial data 204 serial port overrun error Caused by host computer sending too much data and ignoring the flow control signal 205 serial port framing error 206 cmd generated too Firmware error much output 4 8 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual F Maintenance and Troubleshooting Table 4 1 Error Codes Messages and Notes about the Errors nn ERROR MESSAGE NOTES 207 fatal trap Instrument error Instrument locks up 208 RTOS error Firmware error 209 stack overflow Firmware error 210 unknown interrupt Firmware error 300 399 Hardware Errors 300 thermistor faulty Unable to read a reasonable thermistor value Thermistor faulty or disconnected Main board problem or ambient temperature out of range 301 safe temperature limit A temperature of over 50 C detected on one or more of the 4 exceeded thermistors Temperature will be shut off and remain off until a successful completion of power up reset 302 low light Not enough light detected to make an accurate measurement 303 unable to cal dark cur Too much stray light detected on power up fa2. be monitored in the display Wavelength Selection The display on the control panel shows the currently selected measurement wavelength A You can change the wavelength by pressing the up or down arrows until the display shows the desired wavelength The wavelength will wrap around to the top or bottom when the last selectable wavelength is reached Temperature Regulation The SPECTRAmax 190 has been designed to regulate the temperature of the microplate chamber from 4 C above ambient to 45 C Upon powerup when the incubator is off the temperature in the SPECTRAmax 190 microplate chamber is ambient and isothermal Pressing the incubator TEMP On Off key will cause the instrument to begin warming the microplate chamber The temperature set point defaults to 37 0 C at start up Accuracy of the temperature set point is guaranteed only if the set point is at least 4 C above ambient If the temperature set point is lower than the ambient temperature the chamber temperature will remain at ambient Temperature regulation is controlled by heaters only and therefore cannot cool the temperature to a setting lower than ambient Additionally the highest setting 45 C can be achieved only if the ambient temperature is gt 20 C You can change the temperature set point by pressing the up 4 or the down VW arrow keys above and below the word TEMP until the desired set point is shown in the display Typically the microplate chamber
3. or from a local hardware store Make sure fuses are rated SLOWBLOW U S 4 amp time delay metric 4 amp 5 x 20 mm time delay To change fuses follow the steps below Switch power to the instrument off and then remove the power cord from the outlet and from the SPECTRAmax 190 power cord receptacle Remove the printer cable and computer cable if connected from the back of the SPECTRAmax 190 Turn the instrument around for easy access to the rear panel On the left hand side of the rear panel viewed from the back are the power switch fuse box and power cord receptacle As shown in the figures below press to the right of the black plastic cover of the fuse box to release it Pull the fuse box cover away from the instrument The fuse box will begin to slide for ward Fuse Box Cover Figure 4 1 Power Switch Fuse Box and Power Receptacle SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 4 5 Chapter 4 5 Continue gently pulling the fuse box forward until it is free of the instrument Figure 4 2 Removing the Fuse Box When removed the fuse assembly will appear as shown in Figure 4 3 The holder inside contains two fuses It is possible that only one of the fuses may have blown Molecular Devices recommends that you replace both fuses however to ensure continued proper operation Pull both fuses out of the holder and discard them Figure 4 3 The Fuse Box and Holder w
4. 112 134 6 151 111 and 6 188 476 Protected by Canadian Patent 1 305 334 Other US and International patents pending SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual iii Contents Fig re and Table cc e se eek vi Conventions Used in this Manual vii Glossary of Terms eu en k l n bp t Ala vii Chapter 1 Instrument Description 1 1 Introducido es eRe wie 1 3 General Overview iiL ue wring KK pai 1 3 Pahcheck nol 0 RSE eit ee 1 4 Component Description re ovina en cs Ha 1 5 Control Panel mer ser 1 5 Control Panel Display 1 7 The Microplate Drawer 1 7 The Back Pane ya ia pet uz ee 1 8 Functional Description s s Da a a 1 9 Stand Alone Operation uni cs 1 9 Wavelength Selection u E tr ERE 1 9 Temperature Regulation 1 9 Computer Control 7 204028 ee 1 10 PEA rer ey kh ER S e A 1 12 Chapter 2 Installation eese 2 1 Installation Warnings ostia KK eir d 2 3 Installation Cautionsu cs eios tee ice es quld 2 3 Unpacking osie A 2 3 Setting Up for Stand Alone Use 2 4 Chapter 3 Stand Alone Operation 3 1 Prepare for a Reading Eee KK KK 3 3 Turn the Instrument and Printer On 3 3 Set the Temperature lu u ne 3 3 Select the Wavelength 3 3 Operation Overview sta ek kk k kk KK KK
5. 190 System The on board microprocessor calculates and reports the absorbance or Transmittance for each well of amicroplate Data from multiple wave lengths can be acquired during a single reading if desired and different calculations can be made based on this data using SOFTmax PRO software including the subtraction of blanks use of standard curves etc Typical applications include DNA quantitation endpoint assays quantitation of cytoproliferation by MTT reduction colorimetric protein assays and endpoint ELISAs and kinetic measurements enzyme studies such as determination of the activity of enzymes released from cells and kinetic ELISAs All standard 96 well microplates strip wells and filter bottom microplates can be used in the SPECTRAmax 190 With SOFTmax PRO the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle making it possible to per form kinetic analysis of solid phase enzyme mediated reactions mixing is not critical for liquid phase reactions The temperature of the microplate chamber can also be regulated if desired from 4 C above ambient to 45 C SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 1 3 K nm Chapter 1 TM PathCheck As predicted by the Beer Lambert law of light absorption absorbance is pro portional to the distance that light travels through the sample the longer the pathlength the higher the ab
6. C if no setting had been made Pressing the up A or down arrow once in crements or decrements the temperature shown in the dis play by 0 1 C pressing and holding either arrow increments or decrements the temperature shown in the display by 1 C until it is released If you increment the setting to the highest limit 45 C and continue to press the up A arrow the dis play will not change If you decrement the setting to the low est limit 15 C and continue to press the down V arrow the display will not change ANG If the incubator is disabled pressing the Temp On Off key will enable the incubator Selects the wavelength that will be used for reading the microplate manually Pressing the up or down arrow key scrolls up or down through the available wavelengths start ing at the previous setting Pressing the up A or down V arrow once increments or decrements the wavelength shown in the display by 1 nm pressing and holding either arrow increments or decrements the wavelength shown in the dis play by 10 nm until it is released If you increment the set ting to the highest limit 850 nm and continue pressing the up A arrow the display returns to the lowest possible set ting 340 nm and begins incrementing from there The inverse is true for decrementing by pressing the down arrow Setting the wavelength in SOFTmax PRO and reading using the soft ware will override the manual setting The contr
7. Error Code Classifications Not all error messages are listed in this manual The errors are grouped in relationship to possible causes as follows Error Code Numbers Possible Causes 100 199 Errors possibly caused by unrecognized commands being sent from the computer to the instrument 200 299 Errors probably due to a main board failure or an error in the firmware code Most of these errors require the assistance of Technical Support 300 399 Instrument errors due to either a main board failure or other system failure Most of these errors require the assistance of Technical Support 400 499 Errors caused by a motor motion failure Most of these errors require the assis tance of Technical Support 500 599 Errors due to failure or improper initialization of the instruments non volatile memory NVRAM All of these errors require the assistance of Technical Sup port Some errors shown in boldface in this manual are considered fatal if they are detected during power up the instrument will abort the power up sequence and display FATAL ERROR on the LCD panel Check the following table to see if you can do something to prevent the fatal error After correcting the problem leave the instrument on for about five minutes turn it off and then back on If you continue to get the fatal error message on pow erup record the error message number and contact Molecular Devices Techni cal Support or y
8. a reasonable time or can t find its photo interrupt 402 grating error Grating did not move to its photo interrupt in a reasonable time or can t find its photo interrupt 403 stage error Stage did not move to its photo interrupt in a reasonable time or cant find its photo interrupt 500 599 NVRAM Errors 500 NVRAM CRC corrupt The CRC for the NVRAM data is corrupt 501 NVRAM Grating cal Grating calibration data is unreasonable data bad 503 NVRAM Plate air cal Plate air calibration data is unreasonable data error 504 NVRAM Carriage offset Carriage offset data is unreasonable error 505 NVRAM Stage offset Stage offset data is unreasonable error 506 NVRAM Battery Time to replace the NVRAM battery U3 4 10 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual nam S Maintenance and Troubleshooting Opening the If an error occurs while the drawer is closed and you need to open the drawer Drawer press the Drawer key If the drawer does not open turn power to the instru ment off and then on again Manually If the drawer still remains closed turn the power off and using your thumb nail locate the groove in the upper left side wall of the door Open the door and with your index finger pull the microplate drawer out of the instrument do not force the drawer and remove the microplate This action will not harm the instrument but should be taken only if the first t
9. is accomplished only with the use of SOFTmax PRO software SOFTmax PRO software from Molecular Devices provides full instrument control and statistical data analysis 1 4 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Instrument Description Component The main components of the SPECTRAmax 190 are Description The control panel The microplate drawer The back panel connections and power switch Back Panel Control Panel Microplate Drawer Figure 1 2 Major areas of the SPECTRAmax 190 Control Panel Drawer Figure 1 3 Control Panel The control panel consists of an LCD and seven pressure sensitive color coded membrane keys which can be used to perform the stand alone func tionality of the SPECTRAmax 190 including setting the temperature inside the microplate drawer setting the wavelength at which to read the micro plate and opening or closing the microplate drawer Pressing a control panel key activates the function LCD A 2 x 20 character liquid crystal display which shows the current instrument settings SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 1 5 Chapter 1 ee 1 6 Incubator Enables disables the incubator function Incubator Allows you to enter a set point to regulate the microplate chamber temperature Pressing the up or down arrow key scrolls up or down starting at the previous tem perature setting or the default of 37 0
10. sold under this agreement are delivered free of the rightful claim of any third party by way of patent infringement or the like If the Buyer furnishes specifications to the Seller the Buyer agrees to hold the Seller harmless against any claim which arises out of compliance with the specifications Any description of the products contained in this agreement is for the sole purpose of identifying them Any such description is not part of the basis of the bargain and does not constitute a warranty that the products shall conform to that description Any sample or model used in connection with this agreement is for illustrative purposes only is not part of the basis of the bargain and is not to be con strued as a warranty that the products will conform to the sample or model No affirmation of fact or promise made by the Seller whether or not in this agreement shall constitute a warranty that the products will conform to the affirmation or the promise For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers 408 747 1700 800 635 5577 U S and California SPECTRA max Microplate Spectrophotometer 100 240V 4A 50 60 HZ E rT14 0A 2 Lines Fused unplug before servicing Vor Wartungsarbeiten Netzstecker ziehen Molecular Sunnyvale CA Devices Made in USA G9 ru T V Rheinland Protected by US Patents 4 968 148 5
11. those specifically desig nated in the instructions Doing so might cause misalignment and will void the instrument warranty The SPECTRAmax 190 is packed in a specially designed carton Please retain the carton and the packing materials If the unit should need to be returned for repair you must use the original packing materials and carton for ship ping If the carton has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument A EANNA The SPECTRAmax 190 weighs approximately 30 Ib 13 6 kg and should be lifted with care It is recommended that two persons lift the instrument together taking the proper precautions to avoid injury After examining the carton place it on a flat surface in the upright position Open the top of the box and lift the SPECTRAmax 190 along with the packing materials around the ends up and out of the shipping box Remove the pack ing material from both ends of the instrument and set the instrument down carefully SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 2 3 Chapter 2 S Setting Up for Stand Alone Use 1 Place the SPECTRAmax 190 on a level surface away from direct sunlight dust drafts vibration and moisture 2 Turn the instrument around so that the back of the instrument is facing you as shown in Figure 2 1 Power Switch Fuse Box Cover 3 Power Cord nn R
12. will reach 37 0 C in 15 to 30 minutes The microplate chamber temperature is maintained at the set point until you press the incubator TEMP On Off key again turning temperature regula tion off The microplate drawer will open and the temperature within the chamber will begin returning to ambient NOVA Should you turn the incubator back on after a momentary shutdown allow about ten minutes after reaching temperature for the control algorithm to fully stabilize the microplate chamber temperature Temperature regulation within the microplate chamber is achieved through electric heaters a fan efficient insulation and temperature sensors The heat ers are located in the chamber which is insulated to maintain the temperature SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 1 9 Chapter 1 ee set point The sensors also mounted inside the chamber measure the air tem perature The temperature feedback closed loop control algorithms compare the measured air temperature inside the chamber against the temperature set point and use the difference to calculate the heating cycles This technique results in accurate precise control of the chamber temperature with a temper ature variation of the air inside the chamber of less than 1 0 C The tempera ture uniformity within the microplate will depend upon the design materials and or configuration of that component Computer Control The SPECTRAmax 190 is equip
13. 4 4 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Maintenance and Troubleshooting Technical Support Warnings and Cautions This chapter lists maintenance procedures and troubleshooting information includ ing error codes that may be seen followed by their most likely causes and remedies Molecular Devices Corporation is a leading worldwide manufacturer and dis tributor of analytical instrumentation We are committed to the quality of our products and to fully supporting our customers with the highest level of tech nical service In order to fully benefit from our technical services please com plete the registration card and return it to the address printed on the card If you have any problems using the SPECTRAmax 190 Microplate Spectro photometer that are not covered in this chapter in the U S contact our Tech nical Services group at 1 800 635 5577 elsewhere contact your local representative EJGHZVZWN It is your responsibility to decontaminate the instrument as well as any accessories before requesting service by Molecular Devices representatives and before returning the instrument or any components to Molecular Devices Corporation A EZAR All maintenance procedures described in this manual can be safely performed by qualified personnel Maintenance not covered in this manual should be performed by a Molecular Devices representa tive A EARN Removal of protective covers that are marked with th
14. 9 second minimum interval between readings single wavelength 1 column 2 second minimum interval between readings single wavelength Scan Speed 33 K nm min 8 well strip 21 K nm min 16 well strip 135 K nm min 8 well strip 77 K nm min 16 well strip Temperature Regulation Isothermal when temperature regulation is not enabled 4 C above ambient to 45 C when temperature regulation enabled The ambient temperature must be gt 20 C to achieve temperature regulation at 45 C 0 1 C 1 0 C 0 5 C at 37 C 15 30 minutes measured on air after initiation of temper ature regulation 4 sensors 0 2 C regulated Temperature regulation system is continuously monitored and updated Flat bottom microplates with isolated wells Automix with SOFTmax PRO Selectable off once prior to any reading and once prior to and between kinetic readings Selectable 1 to 999 seconds three second default Compatibility Standard and half area 96 well flat bottomed microplates 0 3 mL Chapter 1 Display Operating panel Self diagnosis Spill control Computer interface Printer interface Microplates supported Operating temperature Operating altitude Installation category Pollution degree Operating humidity Storage temperature Size h x w x d Weight Power consumption Line voltage Line frequency General Instrument 2 x 20 character backlit LCD 7 key membrane keypad Continuous on boar
15. KK A KK dnei 3 3 iv SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Chapter4 Maintenance and Troubleshooting 4 1 Technical Support en sa a 4 3 Warnings and Cautions asic ai exec pat em ie aa e aes 4 3 General kl a ea kla teu ts e lege 4 4 Cleanings A A un 4 4 Cleaning the Fan Fer unanunua unuunu rnrn 4 4 Changing the Fuses escurrir KK KK KK 4 5 Error Codes and Probable Causes 4 7 Error Messages uoce eee ques een 4 7 Opening the Drawer Manually 4 11 Appendix A Printers and Cables A 1 Compatible Printers is e doi det a ass A 3 cir Pe A 3 Appendix B Accessories l l B 1 Items Available for Use with the SPECII IATA 190 aan aS tls B 3 hnic EHE I 1 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Figures Figure 1 1 SPECTRAmax 190 a seen KK KK KK KK 1 3 Figure 1 2 Major Areas of the SPECTRAmax 190 1 5 Figure L5 Conteh Panel oe dee perde TREE ei 1 5 Figure 1 4 Microplate Drawers ci sce Bis KK KK KK KK ce gus 1 7 Figure 1 5 Components on the Back Panel of the SPECTRA max 190 en cc ln a SR n H d mel te re it ANN 1 8 Figure 2 1 View of Rear Panel ic a e hs KK KK KK KK KK KK 2 4 Figure 4 1 Power Switch Fuse Box and Power Receptacle 4 5 Figure 4 2 Removing the Fuse Box sa dis caw E treo nd 4 6 Figure 43 The Fuse Box and Holder with Fuses Remove
16. SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual K Molecular Devices Molecular Devices Corporation 1311 Orleans Drive Sunnyvale California 94089 Part 0112 0085 Rev A Molecular Devices Corporation SPECTRAmax 190 Operator s Manual Copyright Copyright 2001 Molecular Devices Corporation Allrights reserved No part of this publication may be reproduced transmitted transcribed stored in a retrieval system or translated into any language or computer language in any form or by any means electronic mechanical magnetic optical chem ical manual or otherwise without the prior written permission of Molecular Devices Corporation 1311 Orleans Drive Sunnyvale California 94089 United States of America Patents The SPECTRAmax 190 and methods are protected by the following patents U S Patents 4 968 148 5 112 134 6 151 111 and 6 188 476 Canadian Patent 1 305 334 Other U S and International patents pending Trademarks SPECTRAplate SPECTRAstrip PathCheck and Automix are trademarks and SPECTRAmax and SOFT max are registered trademarks of Molecular Devices Corporation Apple and Macintosh are registered trademarks of Apple Computer Incorporated All other company and product names are trademarks or registered trademarks of their respective owners Disclaimer Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological d
17. Selection 1 9 Wavelength setting 1 6 Index 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual
18. TRAmax 190 Microplate Spectrophotometer Operator s Manual ii Instrument Description Acquire data from the SPECTRAmax 190 Save data files for in depth analysis at a later time Save multiple microplates with individual template and data analysis pa rameters in one or more experiments in a single data file Pre read microplates Analyze kinetic and spectrum data as it is collected Use the Automix function to shake the microplate at preset intervals thereby mixing the contents of each well highly recommended for ELISAs and other solid phase enzyme mediated reactions Display data on screen Raw values reduced number or raw values with reduced number Raw microplate data in a microplate format Ranged data as integers between 0 and 9 in a microplate format Threshold data as being above below or between set limits in a microplate format Gray scale data in seven shades of gray corresponding to high and low lim its in a microplate format Kinetic or spectrum plots of all 96 microplate wells Enlarge the display of individual well plots and overlay multiple well plots Perform data analysis using SOFImax PRO features Calculate maximum kinetic rates on non linear data Assign plate group or sample blanks Perform pathlength correction based on 1 cm cuvette readings Customize data analysis for each group in the template Create graphs with multiple plots Pick from nine curve fitting routines Analyze
19. d 4 6 Table Table IE Error Codes ac i a HH kl lek kk R bac bnc Kik ek ek 4 8 vi SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual F Conventions Used in this Manual Glossary of Terms The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type Example Drawer Italic and boldface type are used for emphasis Examples Press carefully to engage Do not press down NOVA A note provides information that will help you properly execute an action or procedure A EWE GA Indicates an action or condition that could potentially dam age the instrument or one of its components or could result in loss of data A EARN Indicates a situation that could result in potential injury to a person working with the system GOBZCUSCYZNAB Indicates a condition involving potentially infectious bio logical agents requiring that proper handling precautions be taken Absorbance A The amount of light absorbed by a solution To measure absorbance accu rately it is necessary to eliminate light scatter In the absence of turbidity absorbance optical density A log Ip D I incident light I transmitted light In this manual we use the terms absorbance and optical density interchangeably Optical Density OD The amount of light passing through a sample to a detector relative to the total amount of light available Optical Density inc
20. d diagnostics Drawer mechanism reading chamber assembly is protect ed from accidental spillage by drainage ports 8 pin DIN RS 232 serial double shielding required Parallel 25 pin to Centronics double shielding required All 96 well and strip well microplates including lids Environmental For indoor use only 5t0 40 C lt 2000 m II 2 lt 80 20 to 65 C Physical 8 6 in 22 cm x 22 8 in 58 cm x 15 in 38 cm 30 Ib 13 6 kg lt 250 watts 100 240 VAC 4 A 50 60 Hz SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Instrument Description SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Chapter 1 S 1 16 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Chapter 2 Installation Installation Warnings s sea a KK KK le 2 3 Installation Cautions ser 2 3 UR Pariente a Ai a 2 3 Setting Up for Stand Alone Uac es ta k3 2 4 Chapter 2 S 2 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual F Installation Installation Warnings 1 2 Installation Cautions Unpacking Always make sure the power switch on the instrument is in the OFF position and remove the power cord from the back of the instrument prior to any instal lation or relocation of the instrument Do not operate the instrument in an environment where potentially damag ing liquids or gases are present Do not touch or loosen any screws or parts other than
21. e 25D to Centronics Serial Interface Cable contact Molecular Devices for specific pin out requirements Macintosh Male DB8 to Male DB8 IBM Compatible Male DB8 to Female DB9 custom cable made by Molecular Devices SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual A 3 Appendix A S A 4 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Appendix B Accessories Items Available for Use with the SPECTRAmax190 eee B 3 for Instruments with Serial Numbers Beginning with NN Appendix B S B 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual F Accessories Items Available for Use with the SPECTRAmax 190 Cable RS 232 Macintosh to SPECTRAmax 190 8 pin DIN to 8 pin DIN Cable RS 232 PC to SPECTRAmax 190 9 pin to 8 pin DIN Fuse 4 amp 5 x 20 mm Time Delay 22 Power Cord US Canada Japan Mexico India Power Cord EC1 Germany France Scandinavia Italy Korea N 4400 0037 DUR A 4400 0038 Sr dem k 9000 0133 REES 0200 2405 Power Cord EC2 UK Indonesia Singapore Malaysia Power Cord API Australia Hong Kong China SPECTRAmax Mouse Pad KK KK KK KK KK SPECTRAtest 190 Validation Package SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Part Number eier 9000 0091 le Bas 9000 0149 Tm 4601 0014 SEE 4400 0002 4400 0036 B 3 Appe
22. e High Voltage warning symbol shown below can result in a safety hazard AN A EANNA Always turn the instrument off and remove the power cord and any computer printer cables from the back prior to any mainte nance or installation operation A EARN Never perform any operation on the instrument in an envi ronment where liquids or potentially damaging gases are present A EANNA Risk of electrical shock Refer servicing to qualified person nel ALTO Use of organic solvents such as dichloromethane may cause harm to the optics in the SPECTRAmax 190 Extreme caution is advised when using organic solvents Always use a plate lid and avoid placing a plate containing these materials in the reading cham ber for prolonged periods of time Damage caused by the use of incompatible or aggressive solvents is NOT covered by the instru ment warranty ANA Never touch any of the optic mirrors filters or cables or their housing or manifold The optics are extremely delicate and critical to proper function of the instrument ANUN Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so could cause mis alignment and possibly void warranty SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 4 3 Chapter 4 ee General Cleaning Cleaning the Fan Filter 4 4 Keep the drawer closed when the instrument is not in use The drawer can be opened by pressing the DRAWER bu
23. eceptacle Printer Centronics Computer RS 232 Label re 1g m a X B Figure 2 1 View of Rear Panel 3 Insert the appropriate Macintosh or PC serial interface cable into the RS 232 serial port on the SPECTRAmax 190 Connect the other end to the serial port of the computer 4 Insert the female end of the power cord into the power receptacle at the rear of the SPECTRAmax 190 Connect the male end to a grounded power outlet of the appropriate voltage Molecular Devices recommends that you use a surge protector between the power cord and the grounded power outlet 5 If you are using a printer for reading microplates in stand alone mode locate the printer port 25 pin parallel on the rear panel of the SPECTRAmax 190 Connect one end of the cable to this port and connect the other Centronics end to the printer Load paper into the printer according to the manufac turer s instructions and connect the printer s power cord to the power outlet 6 Turn the SPECTRAmax 190 around so that the control panel now faces you Be sure no cables run beneath the instrument Leave at least three inches between the back of the instrument and the nearest objects or surfaces to ensure proper ventilation and cooling 7 Turn on the power to the SPECTRAmax 190 wait for the microplate drawer to open and remove the tape and protective covering from the drawer subplate 2 4 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Chapte
24. evelopments This manual is subject to change without notice Although this manual has been prepared with every precaution to ensure accuracy Molecular Devices Corporation assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows 1 All parts of the SPECTRAmax 190 Microplate Spectrophotometer are warranted for a period of one 1 year from the original date of delivery 2 Alllabor charges to repair the product for a period of one 1 year from the original date of delivery will be paid by Molecular Devices Corporation 3 This warranty covers the SPECTRAmax 190 instrument only and does not extend to any com puter printer software reagents or disposables used with the instrument ii SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual F Labor and Parts To obtain warranty service during the applicable warranty period you must take the product or de liver the product properly packaged in the original shipping materials and carton to an authorized Molecular Devices Corporation service facility You must call or write to the nearest Molecular Devic es Corporation service facility to schedule warranty service You may call Molecular Devices Corpo ration at the telephone number or address below
25. ith Fuses Removed Insert new SLOWBLOW rated fuses into the fuse holder Either end of the fuse may be forward Insert the fuse box into the opening in the instrument making sure that the fuses are on the right side toward the power receptacle Press the fuse box into place making sure the cover snaps closed Reconnect the power cord to the instrument and to the wall outlet and recon nect other cables previously disconnected SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual F Maintenance and Troubleshooting Error Codes and Probable Causes If a problem occurs during operation that causes an unrecoverable error the instrument will stop and an error code number will be shown in the display on the front panel To correct the problem call your local Molecular Devices representative for assistance Error Messages The LCD will display Fatal Error codes when a situation arises that requires attention Any reading in progress will stop Warning messages do not stop a reading but are logged in the error buffer Warning messages indicate a situa tion that requires attention but is not sufficient to stop or prevent a reading Examples of situations that might cause warning messages are low memory entries being out of range or operations that could result in loss of data These messages are generally self explanatory For assistance regarding warning messages contact your local Molecular Devices representative
26. l flash until the set temperature is reached SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 3 3 Chapter 3 EE 3 Ifthe incubator is on the LCD will show the current temperature along with the temperature set point To change the set point to any setting from ambi ent 4 to 45 C press either the up or down arrow above or below TEMP 4 Select the desired measurement wavelength by pressing the up or down arrow near Scroll up or down through the list of wavelengths shown in the LCD using the up or down arrows until the desired measurement wavelength is highlighted 5 Load the prepared microplate into the chamber Press the READ key Due to the continuous changes in printer technology leading to printer obsolescence and the ability of SOFTmax PRO software to print on PC and Macintosh compatible printers Molecular Devices does not recommend reliance on printing in the stand alone mode 3 4 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Chapter 4 Maintenance and Troubleshooting Technical Support war ee ae KK KK KK KK KK a 4 3 Warnings and Cautions se oeste ae 4 3 Generali n ae RA puro ee ren 4 4 CICA was eGo ek neers non ZA ad 4 4 Changing the Fan Filter u ua ae 4 4 Changing the Fuses Awa se deren 4 5 Error Codes and Probable Causes 4 7 Error Messages pio inete TT a 4 7 Opening the Drawer Manually 4 11 z um Chapter
27. ludes absorbance of the sample plus light scatter from turbidity Transmittance T The ratio of transmitted light to the incident light T 1 T 100 T SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual vii viii SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Chapter 1 Instrument Description It teOO De OTI sa er ta daci as 1 3 General Overview suser ei h wan a w Ma E 1 3 PathCheck M user 1 4 Component Description 5 au v3 aaa ani an KK KK KK KK KK 1 5 Control Panel sadze co uso e E ee wia 1 5 Control Panel Display 1 7 The Microplate Drawer 1 22111 1 7 The Back Panel een 1 8 Functional Description es 223 a 1 9 Stand Alone Operation v ars ra 1 9 Wavelength Selection vl esse 1 9 Temperature Regulation 1 9 Computer Control ci ae n KK 1 10 Species 1 12 Chapter 1 S 1 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual TEA u Instrument Description Introduction General Overview The SPECTRAmax 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations It measures the optical density OD of samples in 96 well microplates at a selected wavelength for a single point in time endpoint over a specified period of time kinetic or over a selected wavelength range spectral scan Figure 1 1 The SPECTRAmax
28. mains in the reading chamber during read cycles Microplate drawer operation varies depending upon the incubator status To open the drawer press the key If the incubator is turned on the drawer will remain open for approximately ten seconds after which a beep ing sound will alert you approximately two seconds before the drawer closes automatically SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 1 7 Chapter 1 ee 1 8 Fuse Box Cover Do not obstruct the movement of the drawer If you must retrieve a plate after an error condition or power outage and the drawer will not open it is possible to open it manually see Chapter 4 Maintenance and Troubleshooting Microplates The SPECTRAmax 190 can accommodate standard 96 well microplates strip wells and filter bottom microplates Not all manufacturers microplates are the same with regard to design mate rials or configuration Temperature uniformity within the microplate may vary depending on the type of microplate used The Back Panel Power Switch Power Cord Receptacle f Printer Centronics Figure 1 5 Components on the Back Panel of the SPECTRAmax 190 The following components are located on the back panel of the SPECTRAmax 190 system e Power switch a rocker switch labeled I O for on and off respectively Power cord receptacle plug the power cord in here e Fuse box cover cannot be opened while the power c
29. ment Turn the instrument over so that it rests flat on the bench Pop the black fan cover off and remove the filter Clean the filter by blowing clean canned air through it or by rinsing it first with water and then with alcohol and allowing it to dry completely Place the clean dry filter over the fan and replace the black cover Turn the instrument back over Reconnect the power cord and cables to the instrument SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual F Maintenance and Troubleshooting Changing the Fuses Fuses burn out occasionally and must be replaced If the instrument does not seem to be getting power after switching it on the LCD shows no display first check to see whether the power cord is securely plugged into a function ing power outlet and into the receptacle at the rear of the SPECTRAmax 190 If power failed while the SPECTRAmax 190 was already on check that the power cord is not loose or disconnected and that power to the power outlet is functioning properly If these checks fail to remedy the loss of power follow the steps listed below to replace the fuses Spare fuses two U S and two met ric are shipped with the instrument The U S and metric fuses are identical except for physical size They may be taped to the back of the SPECTRAmax 190 If you no longer have spare fuses you may obtain new ones from Molecular Devices part numbers 4601 0013 for U S 4601 0014 for metric
30. ndix B S B 4 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual F B Back panel 1 8 Bandwidth 1 13 C Cables 2 4 A 3 connecting 2 4 serial 1 8 Calibration 1 13 Cleaning 4 4 Computer control 1 10 Connections 1 5 1 8 cables 2 4 A 3 printer 1 8 2 4 Control panel 1 5 Control panel display 1 7 D Drawer key 1 6 1 7 1 9 open manually 4 11 E Error Messages 4 7 F Fan filter 4 4 Fuses 4 5 accessing 1 8 I Incubator 1 7 1 9 3 3 Installation 2 3 K Keys control panel 1 5 L Labels 1 8 LCD Liquid Crystal Display 1 5 3 4 4 7 M Microplate 1 3 96 well 1 8 filter bottom 1 8 strip wells 1 8 temperature uniformity 1 8 Microplate drawer 1 5 1 6 1 7 closing 1 8 incubator status 1 7 P Pathlength correction 1 4 Ports 2 4 printer 1 8 serial 1 8 Power cord 1 8 2 4 B 3 Power switch 1 5 1 8 3 3 Printer 2 4 3 3 Printer port 1 8 2 4 R Read mode 1 10 Read the microplate 1 10 Remote control 1 10 S Serial port 1 8 Set point 1 6 Set point temperature 1 7 SOFTmax PRO 1 3 1 4 1 10 Specifications 1 13 Spills 4 4 Stand alone operation 1 9 2 4 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Index 1 T Technical service 4 3 Temperature 1 9 1 10 3 3 equilibration 1 10 3 3 incubator 1 10 3 3 regulation 1 9 set point 1 6 1 10 3 3 setting 3 3 stabilization 1 10 W Warranty i ii Wavelength 3 4 range 1 13
31. ol panel will not show the wavelength selected through SOFTmax PRO READ Drawer Initiates the reading of the microplate in stand alone mode Data is sent to the printer port of the SPECTRamax 190 Opens or closes toggles the microplate drawer Whether or not the drawer will remain open depends on the incubator setting If the incubator is off the drawer will remain open if the incubator is on the drawer will close after approxi mately 10 seconds to assist in maintaining temperature con trol within the microplate chamber SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual MEE Instrument Description If the SPECTRAmax 190 is idle all keys on the control panel are active The instrument will automatically close the microplate drawer for the next reading Control Panel Display actual C set point C The left side of the display shows the temperature both actual and set point and whether or not the temperature is at the set point enunciator blinks if not at set point The temperature of the plate drawer is shown in the SOFTmax PRO software The middle of the display shows the wavelength The Microplate Drawer Al Indicator Figure 1 4 Microplate Drawer The microplate drawer is located on the right side of the SPECTRAmax 190 and slides in and out of the reading chamber Springs on two sides of the drawer automatically position and hold a microplate in the proper position The drawer re
32. ord is plugged in When opened it provides access to the fuse box containing two fuses that are required for operation e Printer port double shielded 25 pin parallel for use in stand alone opera tion plug the 25 pin end of the cable into this port the other Centronics end attaches to the port on the printer Computer port double shielded 8 pin RS 232 serial for use with an exter nal computer plug the 8 pin DIN serial cable connector into this port the other end attaches to the serial modem port of the computer Labels provide information about the SPECTRAmax 190 such as line voltage rating cautionary information serial number etc Record the serial number shown on this label for use when contacting Molecular Devices Technical Services SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual F Instrument Description Functional Description Stand Alone Operation The paragraphs that follow describe functions that can be activated during stand alone operation When operating the SPECTRAmax 190 as a stand alone system you can choose a temperature setting choose a wavelength and make an Endpoint read Data is sent to the printer port of the SPECTRAmax 190 The wavelength is chosen by pressing the up or down arrows beneath the display labeled A Temperature regulation of the microplate chamber can be enabled and set using the keys on the left side of the control panel and actual temperature can
33. our local representative for assistance If the instrument is functioning normally when using SOFTmax 5 y 5 PRO no errors should be in the buffer except error number 100 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 4 7 Chapter 4 Table 4 1 Error Codes Messages and Notes about the Errors ERROR CODE ERROR MESSAGE NOTES 100 199 Unrecognized Command Erro rs Sent from the Computer 100 command not found Command string not recognized 101 invalid argument Command Argument not recognized 102 too many arguments Too many arguments after command 103 not enough arguments Missing arguments 104 input line too long Too many characters in the input line 105 command invalid sys Instrument could not perform the give command because it was tem busy busy doing another task Example Request a wavelength while the monochromator is in motion 106 command invalid mea Instrument could not perform command because a measurement surement in progress was in progress 107 no data to transfer Inputting transfer when there s no data in the buffer 108 data buffer full To many data sets in the buffer Can be caused by setting up a long kinetic and disconnecting computer or if SOFTmax PRO is preempted by another application 109 error buffer overflow More than 65 errors in the buffer clear the buffer 110 stray light 111 invalid read settings 200 299 Firmware Errors
34. ped with an 8 pin DIN RS 232 serial port through which a computer can communicate with and control the instrument SOFTmax PRO Molecular Devices SOFTmax PRO software is a highly integrated program that can be used to control and collect data from the SPECTRAmax 190 SOFTmax PRO makes it possible to access the full capabilities of the SPECTRAmax 190 SOFTmax PRO allows you to Read microplates for endpoint kinetic or spectral scans Use up to six wavelengths for endpoint and kinetic readings Perform Absorbance or Transmittance readings in the 190 to 850 nm range Extend kinetic run times up to 99 hours Select your own read intervals for kinetic runs Specify the duration for Automix before and between readings Read the whole plate or a subset of microplate strips Use PathCheck to normalize the absorbance readings in each microplate well to a 1 cm pathlength Design microplate templates to simplify data reduction Identify groups of wells with labels of your choice Identify individual wells with unique names Blank the entire plate groups and or individual wells Save instrument settings template formats and data analysis parameters for microplates as assay protocol files and recall them for later use Rapid instrument and analysis setup for repeated microplate assays Uniform analysis for equivalent microplates Turn the incubator on or off to control the temperature in the microplate drawer SPEC
35. r 3 Stand Alone Operation Prepare for a Reading en m ta a ees 3 3 Turn the Instrument and Printer On 3 3 Set the Temperature s a 44 04 40 pia KK KK 3 3 Select the Wavelength 3 3 Operation Overview sey cic ti eua nee ine 3 3 Chapter 3 S 3 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Stand Alone Operation Prepare for a Reading Operation Overview This chapter contains operating information for the SPECTRAmax 190 Microplate Spectrophotometer If you are an experienced user of this instrument you can turn to the Operation Overview on page 3 3 for a quick review of the operating steps Only temperature selection and single wavelength readings are available in stand alone mode through front panel control To achieve full use of this instrument connect it to a computer and use SOFTmax PRO software Turn the Instrument and Printer On The power switch for the SPECTRAmax 190 is located on the back panel Press the rocker switch to the ON position The instrument will automatically perform diagnostic checks to ensure that it is functioning correctly Turn the printer on at the same time Set the Temperature If elevated temperature within the microplate chamber is required you should turn on the incubator first allowing enough time for the temperature to reach the set point before performing a reading When you first turn the instrument on up to 30 minutes may be
36. required for the temperature within the chamber to reach the set point To enable the incubator press the incubator TEMP On Off key The LCD display will update to show the temperature set point and the current cham ber temperature To change the temperature set point press the up or down arrows above and below TEMP until the desired temperature set point is shown in the display The microplate chamber temperature will be maintained at the set point until you disable temperature control by touching the incubator TEMP On Off key again When the incubator is off the drawer will open and the temperature within the chamber will begin returning to ambient NOVA Should you turn the incubator back on after a momentary shutdown allow about ten minutes for the control algorithm to fully stabilize the chamber temperature Select the Wavelength Press the up or down arrow keys above and below the A to increment or dec rement the wavelength setting 1 nm increments The following steps provide a quick reminder of the basic operating proce dures required to read a microplate using the SPECTRAmax 190 Turn on the power switch of the SPECTRAmax 190 located on the back panel If you wish to regulate the temperature inside the microplate reading cham ber touch the TEMP On Off key to bring the chamber to the default tem perature of 37 0 C The microplate drawer will close and the indicator on the right of the LCD wil
37. s wavelength range 0 2 nm across all optical channels 0 3 to 4 000 OD 0 001 OD 190 850 nm x 1 0 and 0 006 OD 190 850 nm lt 1 0 and 0 003 OD 0 0576 at 340 nm Instantaneous None continuous referencing of monochromatic output Automatic before first kinetic read and before every end point reading None required Xenon flash lamp 5 watts 1 billion flashes Top down Silicon photodiode Photometric Analysis Modes Stand Alone Single wavelength Absorbance reading of the microplate Using SOFTmax PRO e Express data as Absorbance or Transmittance Single wavelength reading of microplate Multiple wavelength up to six reading of microplate Kinetic and kinetic graphics of microplate Spectral scan 190 850 nm of microplate Measurement Time calibration off SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Instrument Description Read time endpoint Standard read Kinetic read intervals Normal scan Speed scan Reading chamber Range Resolution Accuracy Temperature uniformity at equilibrium Chamber warm up time Temperature regulation Drift Temperature regulation diagnostics Recommended microplate Plate mixing modes Plate mixing duration Microplate SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 96 wells in 9 seconds single wavelength 96 wells in 19 seconds dual wavelength 425 amp 650 nm 96 wells
38. sorbance Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume This variable pathlength makes it difficult to perform extinction based assays and confusing to compare results between microplate readers and spectrophotometers OEB The standard pathlength of a cuvette is the conventional basis for quantifying the unique i absorptivity properties of compounds in solution Horizontal Quantitative analyses can be performed on the Light Path basis of extinction coefficients without standard curves e g NADH based enzyme assays When using a cuvette the pathlength is known and is independent of sample volume so absorbance is proportional to concentration Cuvette In the microplate pathlength is dependent on the Vertical liquid volume so absorbance is proportional to both the concentration and the pathlength of the sample Standard curves are often used to determine analyte concentrations in vertical beam Vertical Light Path photometry of unknowns yet errors can still arise from pipetting the samples and standards The PathCheck feature of the SPECTRAmax 190 automatically determines the pathlength of aqueous samples in the microplate and normalizes the absorbance in each well to a pathlength of 1 cm This novel approach to correcting the microwell Microplate Wells absorbance values is accurate to within 2 5 of the values obtained directly in a 1 cm cuvette This pathlength correction
39. to locate the nearest service facility You must schedule warranty service prior to bringing or shipping the product for servicing At the time of re questing warranty service you must present proof of purchase documentation which includes the date of purchase and Molecular Devices Corporation must have the enclosed Warranty Registration form completed signed and returned by you within ten 10 working days of the date of delivery This warranty covers only defects arising under normal usage and does not cover malfunctions or fail ures resulting from misuse abuse neglect alteration modification or repairs by other than an autho rized Molecular Devices Corporation service facility Repair or replacement as provided under this warranty is the exclusive remedy of the purchaser the Buyer Molecular Devices Corporation the Seller shall not be liable for any incidental or con sequential damages for breach of any express or implied warranty on this product except to the ex tent required by applicable law The Seller specifically excludes all express and implied warranties including without limitation any implied warranty that the products sold under this agreement are merchantable or are fit for any particular purpose except such warranties expressly identified as warranties and set forth in the Seller s current operating manual catalog or written guarantee cov ering such product The Seller also makes no warranty that the products
40. tton Always close the drawer immedi ately prior to switching the instrument off QERDA Wear gloves during any cleaning procedure that could involve contact with either hazardous or biohazardous materials or fluids Periodically you should clean the outside surfaces of the SPECTRAmax 190 using a cloth or sponge that has been dampened with water Do not use abra sive cleaners If required clean the surfaces using a mild soap solution diluted with water or a glass cleaner and then wipe with a damp cloth or sponge to remove any residue Do not spray cleaner onto the instrument If needed clean the microplate drawer using a cloth or sponge that has been dampened with water Should fluids spill in the drawer area when the drawer is out they will be directed to a tray at the bottom of the instrument from which they will exit to the bench or counter beneath the instrument Wipe up any spills immediately Clean only the exterior of the unit and the microplate drawer if necessary Never clean the inside of the instrument Do not allow excess water or other fluids to drip inside the instrument The fan filter on the bottom of the instrument requires periodic cleaning The frequency of the cleaning depends on how dusty your particular lab is and could range from once a month to once every six months Turn power to the instrument OFF and then remove the power cord and cables from the back of the instrument Make sure no plate is in the instru
41. ulty or discon rent nected pre amp boards 304 signal level saturation 305 reference level saturation 306 plate air cal fail low Minimum signal reference ratio not met during air calibration light 307 cuv air ref fail 308 stray light Light leak in reading chamber Could also be a faulty pre amp board 309 front panel not respond LCD front panel bad or disconnected ing 310 311 312 gain calibration failed Power up calibration and check of signal path gain is out of tol erance Could be due to bad or disconnected pre amp or exces sive stray light 313 reference gain check fail Power up check of the Reference amplifier s gain out of toler ance Could be due to bad or disconnected pre amp board or excessive stray light 314 low lamp level warning SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 4 9 Chapter 4 Table 4 1 Error Codes Messages and Notes about the Errors faulty 317 monitor ADC faulty ERROR CODE ERROR MESSAGE NOTES 315 can t find zero order On power up grating motor could not find zero order home position 316 grating motor driver Grating motor didn t move to where it was commanded to in a reasonable time 400 499 Motion Errors 400 carriage motion error Carriage did not move to either of its photo interrupts in a rea sonable time or can t find its photo interrupt 401 filter wheel error Filter wheel did not move to its photo interrupt in
42. unknown samples against a standard curve Analyze and compare data within a plate between plates and between ex periments Multiple print formats Print all or individual sections of the data file Define and print a report containing only selected sections Customize the order of data file sections Export data in tab delimited ASCII format for use with Excel or other data base programs For a complete description of the features of SOFTmax PRO refer to the SOFTmax PRO User s Manual SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 1 11 nam Chapter 1 EE Specifica tions Thermal specifications for microplates used in the SPECTRAmax 190 apply to flat bottom microplates with isolated wells All other microplate specifica tions apply to standard 96 well polystyrene flat bottom microplates NY Technical specifications are subject to change without notice Wavelength range Wavelength selection Wavelength bandwidth Wavelength accuracy Wavelength repeatability Photometric range Photometric resolution Photometric accuracy linearity microplate 0 2 0 OD Photometric precision repeatability 0 2 0 OD Stray light Photometric stabilization Photometric drift Calibration Optical alignment Light source Average lamp lifetime Illumination Photodetectors Photometric Performance 190 850 nm Monochromator tunable in 1 nm increments lt 2 0 nm FWHM full width half maximum 1 0 nm acros
43. wo options have failed to open the drawer If you are still unable to open the drawer contact Molecular Devices Technical Support or your local representative for assistance SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 4 11 z um Chapter 4 4 12 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Appendix A Printers and Cables Compatible lZ pepa ee RR xul keda S et ee A ssh ean oh ee Appendix A S A 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual F Printers and Cables Compatible Printers Cables The SPECTRAmax 190 can be used with an Epson compatible 80 column printer Compatibility may require changing the dip switch or pin settings on the printer or insertion of an emulation cartridge Please check your printer s manual to assess compatibility based on this criterion some printers will not meet this requirement Due to the continuous changes in printer technology leading to printer obsolescence and the ability of SOFTmax PRO software to print on PC and Macintosh compatible printers Molecular Devices does not recommend reliance on printing in the stand alone mode Molecular Devices recommends that you use high quality double shielded cables to connect the SPECTRAmax 190 to other peripheral instruments such as a printer or computer Choose cables that meet the following requirements Printer Cable for Stand alone operation only Centronics parallel mal
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