- Diagenode
Contents
1. CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL PAGE 13 Instruction for use The valve is automatically controlled by the Bioruptor No special management is requested As soon as the Bioruptor and the Water cooler are turned on the water flow starts The Bioruptor automa tically closes the valve while sonication treatment is on his design protects the sonication treatment from any interference that would be generated by the water flow Sonication bath and Water cooler Water level and quality e he sonication bath and the Water cooler must be filled with distilled water only The water level of the sonication bath should always reach the red line sticker on the wall of the tank replacement stickers can be obtained from Diagenode e To obtain the best sonication performance the water in the sonication bath and the Water cooler have to be changed at least once per week Please use the provided plastic pump as shown in the images below to release the water from the Water cooler and sonication bath Water temperature e The water in the sonication bath must be kept at 4 C Ultrasonic waves produced by the Bioruptor gene rate heat A drop off in sonication efficiency will occur above 8 C To ensure preservation of the samples and to prevent damage to the instrument it is necessary to start the sonication process with cold water and to keep it at 4 C during the sonication process Never set the temperature below 4 C for the Wate2. lt diagen dde I Innovating Epigenetic Solutions S ngle Cycle Valve for Bioruptor Version 1 02 04 13 DIAGENODE SINGLE CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL PAGE 3 Contents Introduction 4 Warnings 4 Guarantee 4 Intended use of the equipment 4 Equipment Components 3 Equipment installation for Bioruptor Pico 7 Location requirements 7 Installation 7 Equipment installation for Bioruptor Plus 10 Location requirements 10 Installation 10 Instruction for use 13 Technical assistance 14 Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 4 DIAGENODE SINGLE CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL Introduction Warnings These instructions cannot claim to cover all details of possible equipment variations nor in particular can they provide for every possible example of installation or operation Irouble free and safe operation of the unit is dependent on proper transport and installation by qualified personnel Guarantee Limited one year global warranty Diagenode guarantees its products against possible manufacturing defects in material and workmanship Diagenode products are rigorously tested to ensure that the products you trust meet stringent standards Consequently if a problem occurs with a Diagenode product and the problem is caused by manufacturing defects in material and workmanship Diagenode will in its d
3. 2 7 Cutting Device 1 Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 6 DIAGENODE SINGLE CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL Global scheme Single Cycle Valve al y wired to Bioruptor I t Bioruptor Water Cooler Environmental Conditions Devices are designed to be safe under the following conditions Indoor use Altitude up to 2 000 m Operating external temperature 0 C to 40 C Fluid temperature 4 C to 50 C Maximum relative humidity 80 96 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C without condensation MAINS supply voltage from 100V AC to 230V AC with fluctuations up to 10 of the nominal voltage Transient overvoltages typically present on the MAINS supply Degree of protection IP20 POLLUTION DEGREE 2 Normally only non conductive POLLUTION occurs Occasionally however a temporary conductivity caused by condensation is expected Warning Never use in atmosphere with flammable gas Never use in any location where there is a possibility of extreme dust Environment exempt from sunlight is required Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Li ge Sart Tilman Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SINGLE CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL PAGE 7 Equipment installation for Biorup
4. Roo 4000 Li ge Belgium Tel 32 4 364 20 50 Fax 32 4 364 20 51 ordersediagenode com UCD 300 TO infoediagenode com Diagenode Inc USA NORTH AMERICA 400 Morris Avenue Suite 101 Denville NJ 07834 USA Tel 1 862 209 4680 Faxe 062 2097 68 orders naediagenode com info naediagenode com For a complete listing of Diagenode s international distributors visit http www diagenode com pages distributors html For rest of the world please contact Diagenode s a 2010 Diagenode Inc All rights reserved The content of this document cannot be reproduced without prior permission of the authors Bioruptor and IP Star are registered trademarks of Diagenode Inc
5. cooler Blue connector and blue tubing will carry water from the Water cooler to the sonication bath 8 Plug the Single Cycle Valve cable into the outlet on the back side of the Single Cycle Valve Box and on the Bioruptor Plus 9 Connect the power supply adapter to the control unit with the power cable Power supply adapter is only needed outside the U S Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 12 DIAGENODE SINGLE CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL 11 Press main switch on the front side of the Water cooler Main Switch 12 Fill the tank of the Water cooler with 3 5 liter and the sonication bath of the Bioruptor up to the red line of the sticker 700 or 730 ml depending on the model with distilled water only do not use deionized waterl 13 Temperature can be set to 4 C by pushing the SET key 57 and the arrow key of the control panel at the same time i Display LED display status Li Li 1 L Pump SET A a Arrow keys SET key Start stop key 14 Press Start Stop key to control the temperature Note See Water cooler manual for additional information Now you are ready to start Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Liege Sart Tilman Belgium Phone 32 4 364 20 50 Mail infogdiagenode com DIAGENODE SINGLE
6. cooler below the Bioruptor Location Requirements The Water cooler must be located below the Bioruptor Plus minimum elevation difference 400 mm or 15 74 inch 9 Place the Single Cycle Valve on top of the Water cooler as shown in the image Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Liege Sart Tilman Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SINGLE CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL PAGE 11 6 Insert the short red and blue tubing into the outlets of the Water cooler and the lower red and blue nozzle connectors Ec of the single cylce vavle box as shown in the image Respect the red and blue color codes 7 Connect the Bioruptor Plus to the Water cooler with the long red and blue tubing by inserting them into the upper red and blue nozzle connectors y of the single cycle valve box as shown in the image Respect the red and blue color codes Optional Cut the length you need for the output and input flow Make sure there is enough slack I Important Note If you need to cut a part of the tubing always use the provided Cutting Device Never use scissors because pieces of tubing with bad cuts generate leaks at junctions Only properly cut tubes can be inserted in the connectors Note Red connector and red tubing will carry water from the sonication bath to the Water
7. eed to cut a part of the tubing always use the provided Cutting Device Never use scissors because pieces of tubing with bad cuts generate leaks at junctions Only properly cut tubes can be inserted in the connectors Note Red connector and red tubing will carry water from the sonication bath to the Water cooler Blue connector and blue tubing will carry water from the Water cooler to the sonication bath 7 Plug the Single Cycle Valve cable into the outlet on the back side of the Single Cycle Valve Box and on the Bioruptor Pico 9 Plug the power cord into the outlet of the Water cooler Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Liege Sart Tilman Belgium Phone 32 4 364 20 50 Mail infogdiagenode com DIAGENODE SINGLE CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL PAGE 9 10 Press main switch on the front side of the Water cooler Main Switch 11 Fill the tank of the Water cooler with 3 5 liter and the sonication bath of the Bioruptor up to the red line of the sticker 700 or 730 ml depending on the model with distilled water only do not use deionized waterl 11 Temperature can be set to 4 C by pushing the SET key ET and the arrow key of the control panel at the same time i Display LED display status unc 1 0 E Pump SET N ius Arrow keys SET key Start stop key 12 Press Start Stop key to control the temperatu
8. iscretion either fix or replace the product in accordance with the warranty terms and conditions stated herein The warranty applies only to the first purchaser of the product for a period of one year starting from the date this product was delivered In case of repair or replacement on a product under warranty expenses will be at Diagenode s charge including any costs required to return the repaired or replacement product to you This warranty covers only manufacturing defects and does not cover any damage caused by misuse non respect of recommendations described in this manual neglect accidents abrasion exposure to extreme temperatures solvents acids Intended use of the equipment The Single Cycle Valve for Bioruptor as well as all components from this parcel including plastic tubings and strips must be used as a junction between the Water cooler and a Bioruptor This equipment is designed to be used in the above described application only Any medical application is out of scope Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Li ge Sart Tilman Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SINGLE CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL PAGE 5 Equipment Components Component Quantity 1 Single Cycle Valve 1 2 Tie Wraps 10 3 Tubing blue and red long 2 4 Tubing blue and red short 2 5 Cable 1 6 Plastic Junction for Bioruptor Plus only
9. r cooler since this will damage the machine severely Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 14 DIAGENODE SINGLE CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL Technical Assistance amp Ordering Information Diagenode s a BELGIUM EUROPE Diagenode Inc USA NORTH AMERICA Avenue de l h pital 1 400 Morris Avenue Suite 101 Tour GIGA 3rd Floor Denville NJ 07834 USA 4000 Liege Belgium Tel 1 862 209 4680 Tel 32 4 364 20 50 Fax 1 862 209 4681 Fax 32 4 364 20 51 techsupport na diagenode com techsupport diagenode com orders na diagenode com orders diagenode com For a complete listing of Diagenode s international distributors visit http www diagenode com pages distributors html For the rest of the world please contact Diagenode s a Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Li ge Sart Tilman Belgium Phone 32 4 364 20 50 Mail info diagenode com el 70 0 LA A9S VW Ordering information Description Cooling System Single Cycle Valve Water cooler Bioruptor Pico Bioruptor Pico sonication device for 0 65 ml tubes Bioruptor Plus Bioruptor Plus sonication device with 1 5 amp 15 ml tube holder diagengte Innovating Epigenetic Solutions Cat No B02020004 BioAcc cool Diagenode s a BELGIUM EUROPE Avenue de l h pital 1 B01060001 o GEA sige
10. re Note See Water cooler manual for additional information Now you are ready to start Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 10 DIAGENODE SINGLE CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL Equipment installation for Bioruptor Plus Before starting the installation turn the main switches off and make sure that the unit is not plugged into an electrical outlet 1 Open the boxes and unpack all components Water cooler Bioruptor Plus Single Cycle Valve BioAcc cool UCD 300 B02020004 2 Place the 2 black plastic connectors on the motorized lid Both connectors can be placed into one or the other hole of the motorized lid Screw the plastic nut over the motorized lid The short white pieces of tubing have to be inserted in the plastic connectors i im 3 Place the Bioruptor on a bench and position the sonication bath in front of the soundproof box upon the final site Connect the Bioruptor sonication bath to the Water Cooler with the red and blue tubes by inserting them in the appropriate connectors Optional Cut the length you need for the output and input flow Make sure there is enough slack The red and blue tubes must go through the soundproof box holes before being connected to the motorized lid see picture Important Note Please make sure that the Bioruptor Plus is always placed on a level surface 4 Place the Water
11. tor Pico Before starting the installation turn the main switches off and make sure that the unit is not plugged into an electrical outlet 1 Open the boxes and unpack all components Water cooler Bioruptor Pico Single Cycle Valve BioAcc cool B01060001 B02020004 2 Place the Bioruptor on a bench Important Note Please make sure that the Bioruptor Pico is always placed on a level surface 3 Place the Water cooler below the Bioruptor Location Requirements The Water cooler must be located below the Bioruptor Pico minimum elevation difference 400 mm or 15 74 inch 4 Place the Single Cycle Valve on top of the Water cooler as shown in the image Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 8 DIAGENODE SINGLE CYVLE VALVE FOR BIORUPTOR PICO USER MANUAL 5 Insert the short red and blue tubing into the outlets of the Water cooler and the lower red and blue nozzle connectors Ec of the single cylce vavle box as shown in the image Respect the red and blue color codes 6 Connect the Bioruptor Pico to the Water cooler with the long red and blue tubing by inserting them into the upper red and blue nozzle connectors y of the single cycle valve box as shown in the image Respect the red and blue color codes Optional Cut the length you need for the output and input flow Make sure there is enough slack Important Note If you n
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