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VersaDoc - St. Michael`s Hospital

1. Threaded insert for captive screw on the EPI UV Slots for EPI UV Tongue and Groove mechanism to hang EPI WHITE from EPI UV module Figure 3 19A Figure 3 19B 6 Next attach the epi white module to the epi UV module by locking in the tongue Users Manual 3 18 and groove located at the outer top edges of each module Figure 3 19B 7 Now secure the epi white module to the epi UV module using the latch and screw mechanism located at the front plate of the modules Figure 3 19C Figure 3 19C 8 Next connect the cable from the epi white module to the appropriate connector on the ballast assembly 9 Re install the access panel to the ballast panel NOTE The plugs for each illumination module are keyed and cannot be interchanged Each plug will snap together only to the appropriate socket 3 2 7 Electrical and Communication Connections Power The power entry module of the VersaDoc system is configured for operation at 100 120 VAC 60 Hz or 220 260 VAC 50 Hz After checking that the system s power switch is turned off insert an approved power cord into the power entry module located on the bottom right of rear panel of the unit Figure 3 20 Warning This instrument must be connected to an appropriate AC voltage mains outlet that is properly grounded Warning There are hazardous voltages inside the rear panel of the VersaDoc Do not remove the cover to the electronics back panel when the instrument
2. ZoomLens 50 mm Fixed focal length lens Figure 4 6 Zoom and fixed zoom lenses 4 3 5 Lens Adjustments and Aperture Adjustment The aperture or f stop ring is located at the top of the lens Figure 4 6 and controls the amount of light that passes through the lens to be captured by the CCD camera When the aperture is fully open the f stop number will be smallest the depth of field will be the lowest and the most light will pass through the lens When the aperture is fully closed the f stop number will be largest the depth of field will be highest and the least light will pass through the lens For low signal applications such as chemiluminescence it is recommended that the aperture be fully opened to the smallest f stop value Note When the aperture on the zoom lens is fully closed largest f stop setting of 22 the aperture ring locks To unlock the aperture ring push the black release button to the right of the f stop indicator while turning the ring to the right counter clockwise Note The aperture ring on the 50 mm lens will not lock in the fully closed f stop 16 position unless the user activates the lock mechanism on the lower right hand side of the f stop indicators To lock in this position move the button white dot up so that it aligns with the orange indicator It is not recommended that the lens be locked in this position for chemiluminescence experiments as this setting allows the least light through the lens
3. 3 2 4 Installing VersaDoc 1000 3000 4000 MP4000 Connecting the Cables to the Camera Module This procedure applies to the Versa Doc 1000 3000 4000 and MP4000 systems User s Manual 3 7 Connecting the camera power cable Camera power cord has a DIN connector on each end The cable with a 9 pin DIN connector is for the VersaDoc 1000 4000 and MP4000 cameras and the cable with a 15 pin DIN for the VersaDoc 3000 Figure 3 2 and Figure 3 3 NOTE VersaDoc MP4000 systems come with a separate power supply and power cable for the camera VersaDoc 3000 systems with Enclosures that have serial number XXXBR 300 or greater also come with a separate camera power supply and power cable For the VersaDoc MP4000 and VersaDoc Systems with enclosures that have serial number above XXXBR300 then connection should be made per pictures shown below Figure 3 2 VersaDoc MP4000 Camera Power Connector VersaDoc 1000 and 4000 VersaDoc 3000 Camera Power Connector Camera Power Connector Camera Power connector Camera Power connector on the top side of the rear on the cable from camera panel power supply Camera Power connector on the cable from camera power supply Figure 3 2 VersaDoc MP 4000 VersaDoc 1000 3000 and 4000 power connections User s Manual 3 8 For enclosures with serial numbers below XX XBRO300 connect the power cable between the camera power socket on the camera module and the enclosure Pictures below are for
4. Molecular Imager TM VersaDoc MP VersaDoc Imaging Systems User s Manual for Catalog Numbers 1708640 1708650 1708010 1708011 1708030 1708031 1708140 1708141 1708050 1708051 Copyright 2001 Bio Rad Laboratories Inc Manual Part Number 4000183 Rev F Welcome Dear Customer On behalf of Bio Rad Laboratories we would like to thank you for investing in the Molecular Imager VersaDoc and VersaDoc Imaging Systems and we are sure that it will provide you with many years of high quality imaging One of the best ways to familiarize yourself with the capabilities of your new VersaDoc system is to read this manual In it you will learn how to set up the system and operate all hardware components It is also recommended that you read the accompanying software manual to familiarize yourself with general acquisition functions and data analysis After reading this manual please keep it close to your system so that it can be conveniently referred to Your VersaDoc system is protected by a comprehensive instrument warranty agreement Please read this manual thoroughly so that you fully understand the coverage provided and are aware of your rights and responsibilities One of the responsibilities of system ownership is regular maintenance Following the maintenance instructions provided with this manual will help to keep your system and peripherals functioning optimally and will protect your investment Please also
5. User s Manual 4 7 Zoom Adjustment When using the zoom lens the view area of the image can be adjusted by rotating the zoom ring Figure 4 6 The zoom ring is located directly below the aperture ring At maximum zoom the indicator on the lens will be set to its highest specified focal length 40 or 80 mm depending on which lens is being used also the image resolution will be at its best The zoom setting can be adjusted and viewed in real time by selecting the POSITION function in the VersaDoc acquisition window The image display window will now show a real time image of the sample as it will be captured This image refreshes every second to help you optimize your zoom settings Note The 50 mm lens offers no zoom adjustment Focus Adjustment To focus the lens on the sample for sharp images you must choose the FOCUS button in the Quantity One acquisition window This button allows focusing on a small part of the image When using this mode the white epi lights turn on automatically and the shutter is opened and closed repeatedly and the acquisition window is continuously updated to allow you to focus your lens on the sample The exposure time during the focus mode is adjusted automatically to allow proper unsaturated images The Highlight Saturated Pixels can be selected in the acquisition window to help determine the level of saturation during the focusing process If the images still show up saturated while focusing then you may turn
6. 3000 4000 and 5000 Systems To optimize image quality it is recommended that the filtered glass covering of each epi illumination module is cleaned periodically using an optical cleaning solution and optical tissue Avoid touching the glass surfaces of the epi illlumination modules when installing it into the instrument as fingerprints will affect image quality 5 2 Replacing Lamps in Illumination Sources The life of the broad wavelength UV lamp is approximately 500 to 1000 hours depending upon use As the lamp ages the required integration time will increase because the intensity of the UV emission will diminish If the integration time for image acquisition has increased more than three fold it is recommended that the lamps be replaced The life of the white light lamp is approximately 1000 hours NOTE The lamps should be replaced in pairs from all illumination modules 5 2 1 Replacing the Lamps in the Transilluminator Module To access the lamps in the transilluminator follow the procedure outlined below Caution Do not touch the glass parts of the lamp or lamp housing Fingerprints on the lamp may result in non uniform illumination The use of powder free latex gloves is highly recommended Tools required You will need a 2 Phillips screwdriver and 5 16 size socket wrench to change the lamps in the transilluminator To change the lamp follow the procedure outlined below Figure 5 1 Turn off the power to the system
7. Omega series filters see website www omegafilters com for more information Mechanical Specifications Laminated construction edges sealed Enclosure Black anodized aluminum ring 921 0136 Diameter 25 4 0 0 25mm Thickness 4 4 0 1mm without ring 4 8 0 1mm with ring Clear aperture 21 4 0 1 Omm TWFD lt Lambda 4 per 25mm For dual imaging applications filters must be ordered in matched sets Max difference in thickness between filters without ring in a set 0 1mm CiCheck this box for each dual imaging filter ordered Optical Specifications Wavelength Type Check one gt 450 nm C BP Bandpass C WB Wideband O DF Discrminating C CF Cut on C EF Edge 50 Transmission Point 10mm Transmission Band Minimum to nm gt 80 Avg Blocking Minimum O D gt 5 0 from cut on to UV Edge slope Per Omega published data for EF series Each filter whose construction incorporates absorbing glass shall be oriented with the absorbing glass facing the rim of the aluminum ring This orientation shall be clearly marked on the edge of the holder with an arrow The head shall point toward the absorbing glass The filter shall be used in an orientation with light going in the direction of the arrow Thus the light shall first strike the side furthest away from the absorbing and possibly fluorescing glass and autofluorescing shall be minimized Absorbing side FAX order form to Omega
8. Open the door and remove ballast cover to access the UV transilluminator cable Disconnect the UV transilluminator cable from the ballast assembly Slide the UV transilluminator module out completely There is a release lever on each rail that is made out of black plastic Pull the transilluminator module out while pressing down on the lever on the right side and lifting up the lever on the left This will allow you to completely disengage the slide rails from the guides rails located in the enclosure and the entire transilluminator module will become free from the enclosure Oe Ne User s Manual 5 2 10 11 12 Release lever for Slides Transilluminator Transilluminator without the cover Figure 5 1 Removing UV transilluminator 6 Using the Phillips screwdriver remove the cover assembly from the transilluminator by removing four screws There are two screws on each side of the cover Note Be careful and do not damage the wires leading from the switch on the cover 7 Using the 5 16 socket wrench remove the four hex standoffs that hold the baffles on the lamps of the transilluminator and then gently lift off the baffles exposing the transilluminator lamps Note Be careful and do not bend the baffles because bending the baffles could lead to deterioration of uniformity of your UV transilluminator 8 Remove lamps by holding the metal part of the lamps with the tip of your fingers and turning them in a rotating mo
9. which is located on the lower right hand side of the system s rear panel The instrument should be placed where there is adequate room to insert the samples into the front of the enclosure and where it can be easily connected to the host computer The maximum distance between the host computer and the instrument should be two meters Instrument is supplied with cables long enough for such distances Note The host computer should be located at a workstation that minimizes operator fatigue 3 1 2 Power Requirements The VersaDoc MP and VersaDoc systems and its host computer should be connected to a stable grounded power outlet on a circuit free of electrical noise In addition a high quality electrical surge suppressor line filter with a 10 Amp or higher rating should be used to avoid damage from AC fluctuations Only a grounded 3 pin power cord should be used to connect power The VersaDoc MP and VersaDoc systems are designed for operation at an input voltage of 110 240 VAC at 50 60 Hz Deviation from these operating voltages can lead to a slower temperature control process with your cooled CCD camera User s Manual 3 1 3 1 3 Host Computer Recommendations The VersaDoc MP and VersaDoc systems are capable of producing large image files of high resolution To easily handle such large files a powerful computer is required Please refer to your software manual for detailed host computer system and software requirements If the compu
10. Cables 1 Connect the AIA cable to the connector labeled AIA on the CEU connector panel The other end of this cable will be connected to the PCI digitizing card in your PC 2 Remove the shorting plug from the camera and connect the camera control cable between the camera and the CEU Figure 3 11 Shorting Plug Fan Assembly Power Connector VesaDoc 5000 MP 5000 Camera Module Shorting plug Figure 3 11 CEU blue shorting plug 3 Connect the AC power cord to the AC inlet on the CEU but do not power up the CEU yet 4 For units serial number below XXXBR300 Connect the fan power cable from the fan assembly 5000 models only to the DB9 power connector located at the top surface of the electronics module Figure 3 12A User s Manual 3 13 5 For VersaDoc MP 5000 and VersaDoc 5000 units with serial number xxxBR300 or higher Connect the fan power cable from the fan assembly 5000 models only to the DB9 power connector located on the side of the electronics module Once connected the system cable connections will look similar to the ones shown in Figure 3 12A or B depending on the serial number of the enclosure you may have Fan Cable Fan Cable Figure 3 12 A 3 12B 3 2 6 Installing the Optional Epi modules on the VersaDoc MP Systems Tools required You will need a size 3 32 hex allenkey and a 2 Phillips screwdriver to install the LED modules in the epi LED assembly 3 2 6 1 Installing EP LED Modules i
11. LED indicator fails to illuminate and the instrument is inoperative check that all power cables are firmly attached and that power is being supplied to the unit If the unit still remains inoperative or the VersaDoc acquisition window cannot be opened on the host computer please contact the Bio Rad Technical Support Department for assistance The VersaDoc should be switched on at least 30 seconds before the host computer to allow for complete initialization If the VersaDoc is not fully operational before the computer is turned on the system will not be recognized as an attached Serial USB device and the camera may not be recognized by the digitizing card thus you will not be able to communicate with the VersaDoc from the Quantity One acquisition window For best imaging results it is recommended that the VersaDoc be allowed to stabilize for 10 minutes before use 4 2 Overview of Operational Steps The user will typically complete the following series of steps when acquiring an image using the VersaDoc 1 Start the program and open the acquisition window 2 Select the desired application 3 Place the sample on transilluminator platen converter screen or chemi tray as the case may be and optimize its position Focus Adjust the lens aperture and zoom Readjust focus if required Select the exposure acquisition time Acquire the image ee or oS User s Manual 4 1 4 3 Detailed Operating Procedures 4 3 1 Opening the Acq
12. Optical 3 Grove St P O Box 573 Brattleboro VT 05301 Phone 802 254 2690 FAX 802 254 3937 Bio Rad order form 410 0167 Rev A User s Manual 6 11 BIO RAD Life Science Group Bio Rad Laboratories Web site www bio rad com Bio Rad Laboratories Main Office 2000 Alfred Nobel Drive Hercules CA 94547 Ph 510 741 1000 Fx 510 741 5800 Also in Australia Ph 02 9914 2800 Fx 02 9914 2889 Austria Ph 01 877 89 01 Fx 01 876 56 29 Belgium Ph 09 385 55 11 Fx 09 385 65 54 Brazil Ph 55 21 507 6191 Canada Ph 905 712 2771 Fx 905 712 2990 China Ph 86 10 8201 1366 68 Fx 86 10 8201 1367 Denmark Ph 45 44 52 1000 Fx 45 4452 1001 Finland Ph 358 0 9 804 2200 Fx 358 0 9 804 1100 France Ph 01 47 95 69 65 Fx 01 47 41 9133 Germany Ph 089 318 84 177 Fx 089 318 84 123 Hong Kong Ph 852 2789 3300 Fx 852 2789 1257 India Ph 91 124 63981 12 1 13 114 Fx 91 124 6398115 Israel Ph 03 951 4124 Fx 03 951 4129 Italy Ph 34 91 590 5200 Fx 34 91 590 5211 Japan Ph 03 581 1 6270 Fx 03 5811 6272 Korea Ph 82 2 3473 4460 Fx 82 2 3472 7003 Latin America Ph 305 894 5950 Fx 305 894 5960 Mexico Ph 52 5 534 2552 to 54 Fx 52 5 524 5971 The Netherlands Ph 0318 540666 Fx 0318 542216 New Zealand Ph 64 9 4152280 Fx 64 9 443 3097 Norway Ph 47 23 38 4 1 30 Fx 47 23 38 41 39 Russia Ph 7 095 979 98 00 Fx 7 095 979 98 56 Singapore Ph 65 2729877 Fx 65 2734835 Spain Ph 34 91 590 5200 Fx 34 91 590 5211 Sweden
13. Ph 46 0 8 55 51 27 00 Fx 46 0 8 55 51 27 80 Switzerland Ph 061 717 9555 Fx 061 717 9550 United Kingdom Ph 0800 181134 Fx 01442 259
14. Rad to schedule a service appointment The instrument should not be modified or altered in any way Alteration of this instrument voids the manufacturer s warranty and may create a potential safety hazard for the user Caution If the door interlock is defeated there is a possibility of Class 2 Laser Radiation hazard due to bright LED illumination Caution must be exercised when servicing the instrument Do not look directly at the LED without proper eye protection Caution Bio Rad is not responsible for any injury or damage caused by the use of this instrument for purposes other than that for which it is intended or by the modification of this instrument when not performed by qualified Bio Rad personnel or an authorized agen Caution Ensure that all of the systems ventilation openings are free of interference Excessive heat build up in the instrument may effect performance or cause operational failure User s Manual 1 2 1 2 2 General Warnings Warning This instrument must be connected to an appropriate AC voltage outlet that is properly grounded Warning There are hazardous voltages inside the rear panel of the VersaDoc Instrument Do not remove the cover to the Electronics back panel when the instrument is connected to AC power Warning Do not defeat any instrument interlocks they are designed to prevent user injury Warning The VersaDoc instrument heavy It weighs up to 44 kg 96 Ibs depending on which camera
15. VersaDoc enclosures with serial numbers below XXXBRO300 Figure 3 3 _ VersaDoc 1000 and 4000 Camera Power Connector 15 pin camera power connector on top side _ of the rear panel Figure 3 3 Power connections for SN XXXBR0300 and below Connecting the AIA CABLE For VersaDoc 1000 3000 4000 and MP 4000 only 1 Connect the AIA cable Part 800 0247 to the AIA port on the camera Figure 3 4 Push the cable to the connector such that it clicks and snaps in place properly dla CONNECTORS WERSAO0C 1000 4000 MPA000 VWERSSOOC 3000 COMERS ON EACH ENO OF COMERS AIA CONNECTOR O10 CONNECTOR THE C4 BLE Figure 3 4 AIA cable connection 2 The other end of the AIA cable will be connected to the PCI digitizing card that will be installed in the PC after software installation as shown later in this procedure User s Manual 3 9 NOTE The steps describing the installation procedure for the PCI digitizing card follows later in this procedure 3 2 9 Installing VersaDoc 5000 and MP 5000 Connecting the cables to the camera module 1 The VersaDoc 5000 and MP 5000 camera modules come with a camera electronics unit CEU and a controller cable See figure 3 5 below Camera Module Camera Electronics Unit CEU Camera Controller Cable Figure 3 5 VersaDoc 5000 and CEU 2 Couple the VersaDoc 5000 and MP5000 camera module to the enclosure as shown in the section 3 2 3 above See Figure 3 1 for
16. be removed from the glass or plastic plates of the gel sandwich for good image quality The glass and plastic will fluoresce when exposed to UV light and will contribute to background signal Sample Chemi Tray It is recommended that the Sample Chemi tray Figure 4 2 be used for all small chemiluminescence samples 8 x 8 cm or less This tray slides onto the guides on the epi illumination assemblies and allows the sample to be placed closer to the camera Large chemi samples should be imaged on the platen To insert and remove the chemi tray the door to the enclosure must be fully opened Note When using the VersaDoc for non chemiluminescence applications the sample tray should be removed from the enclosure as it will block sample signal from reaching the CCD camera User s Manual 4 5 When using white light epi illumination the sample tray should be placed on the dark reflective platen of the transilluminator to avoid unwanted reflections on your image Fig 4 4 VersaDoc chemi sample tray 4 3 4 Lens Selection The VersaDoc system is supplied with two standard lenses a flexible zoom lens and a high numerical aperture 50 mm lens with improved collection efficiency For optimal image acquisition it is recommended that the zoom lens be used for all fluorescence and colorimetric applications Install the 660 nm cut off filter on the zoom lens if you have a VersaDoc 1000 3000 4000 and 5000 The zoom lens can also be used fo
17. is connected to AC power Power entry module at the Power entry module bottom right of the rear panel Figure 3 20 Power entry module on VersaDoc instrument Users Manual 3 19 VersaDoc Connections The VersaDoc is connected to the host computer via a USB or serial and an AIA interface One each of the Serial and USB port is located on the rear right side of the unit Use the supplied USB or Serial Cable to connect the PC to the VersaDoc Caution To prevent damage to the hardware all instruments must be turned off before attempting to connect or disconnect the unit to the host computer Upon AN insertion of the Serial or USB cable into the VersaDoc the Serial or USB port on the PC will automatically select operation and sense the presence of the VersaDoc NOTE Do not turn ON the power to the VersaDoc and CEU in case of VersaDoc MP5000 and VersaDoc 5000 yet Connecting the AIA Cable to the Computer To do this two things must happen I The software must be installed on the PC which will control this system ii The PCI Digitizing card must be installed into the PC where the AIA cable is to be connected Software Installation Please follow the instructions below to install the PC or Mac software for VersaDoc system NOTE Do not install the PCI card into the PC yet First install the drivers as shown below and then install the PCI Card PC based VersaDoc systems 1 Insert The Discovery Series
18. place Be careful not to touch any glass surfaces of the lamp or the filter glass Use of gloves is highly recommended 8 Carefully rotate the lamp to make sure it is seated properly 9 Now reinstall the filter glass end piece and the end plate Secure the end plate properly using the 3 cap head screws and the 3 32 hex key 10 Reinstall the epi module in its place and reconnect epi cables to the ballast assembly to appropriate connectors 11 Re install ballast cover 12 Now power up the system and try running the application that uses the epi source in which the lamps were installed to make sure that the lamps are operating NOTE The UV interlock on the door will not allow UV lights to turn on when the door is open Acquire an image of the Fluorescent Reference Plate to ensure that the UV lamps you installed are working properly If an evenly illuminated image is not acquired you may have a lamp poorly seated in its socket To order replacement lamps contact your Bio Rad Laboratories representative for parts 5 3 Lens and Filter Storage Always store unused lenses and filters in their original box and in a low humidity environment with a stable ambient temperature that does not exceed 30 C Lenses should be wrapped in optical tissue before being placed in their storage box If your laboratory is a high humidity environment it is recommended that all emission filters are stored in a sealed container with desiccant User s Manu
19. search for the drivers and then render the card as DISABLED To recover from this take the following steps Install the drivers as shown in the procedure above 2 Go to ADD REMOVE HARDWARE utility in the CONTROL PANELS 3 Click and highlight the Roper PCI card in question and click on REMOVE Follow the prompts and reboot the system 4 Windows will now find the card and install the drivers as described above User s Manual 6 3 6 2 Technical Service For technical assistance with the VersaDoc system including all hardware and software contact your local Bio Rad office or in the US call 1 800 424 6723 All spare parts not listed in this document can be ordered by contacting your local Bio Rad office For inquiries and requests regarding system repair or service contact your local Bio Rad office or distributor in the U S call Technical Service at 1 800 424 6723 Please have the following details available 1 Instrument model and catalog number 2 Serial number located on the back of the door 3 Hardware and firmware version information can be found by clicking the OPTIONS button in the acquisition window E VersaDoc Options Information Model VersaDoc 5000 MP Firmware Version 1 0 Hardware Version 1 0 White LED Light Available Red LED Light Available Green LED Light Available Blue LED Light Available UY Trans Light Available Dark Subtraction Type Normal Refer
20. side 7 Fuse holder l g Wo x NHIN Figure 3 8 CEU PCB and fuse holder 4 Using the plastic stub on the PCB select the orientation of the board so that the desired voltage numbers and arrow point towards the socket for the board The tab on the inside surface of the stub must match with the notch on the tiny PCB See Figure 3 9a and 3 9b Plastic stub that shows through the hole Indicating the selected voltage 100 VOLT and arrow must point inward Insert the board back into the socket With the letters facing the right side Notch on each side ed E T 4 ag of the PCB i _ 230 VOLT and arrow mist point inward Insert the board back into the socket with the letters facing the right sick NOTCH on each side of the PCE 230 Volt setting Figure 3 9b CEU PCB setting for 230 volt operation 6 Now reinstall the tiny PCB back into it s socket such that the numbers on it face towards the right side towards the Fuse holder inlet 7 Next remove the Phillips screw that holds the fuse holder and turn the holder User s Manual 3 12 around so that the two 1 amp fuses face towards the socket See Figure 3 10 rF Figure 3 10 CEU fuse holder 8 Push the tab until it locks in its socket 9 Check again to make sure that the plastic stub indicates the desired voltage selection Connecting the VersaDoc 5000 MP 5000 Camera and CEU
21. software CD into the CD ROM drive of the PC It will go into AUTO RUN mode and the following window will open User s Manual 3 20 ie Quantity One Setup i S x Welcome to the Quantity One Installation Wizard lt is strongly recommended that you cxit all Windows programs before running this setup program IG Chick Cancel to quit the setup program then close any programs You have runniag Click Nest to continue the installation Discovery WARNING This program is protected by copyrigh law and international treaties Series Unauthorized reproduction or distribution of this program or any portion of it may result in severe civil and criminal penalties and will be prosecuted to the maximum extent possible under law Back 5 zi Select Features Please select which features and instrument drivers you want to install a witha Quantity One Feature Description HSF Suport Support drivers for the Hadware Security Kep Gel Doc EQ ChemiDoc EQ Drivel HSE Chenu SASA a saD or Dives CFR Serice This feature wall be installed on the local hard drive This feature requires 1 744KB on your hard drive Browse Wize Installation wizard Disk Cost Heset lt Back Cancel 3 Click on the arrow next to ChemiDoc XRS VersaDoc drivers to get installer options See picture below ETE pm E a EF i Quantity One Setup IO x Select Features F Please sele
22. tray platen is clean Clean using an optical cleaning solution and a soft lint free towel or lens cleaning tissue if needed The sample tray has a notch on one corner Center your sample on the sample tray so the notch is oriented on the upper right corner Secure the sample in place using the sample holders if necessary Top View Bottom View Fig 4 2 VersaDoc MP sample tray User s Manual 4 3 2 Open the door Locate the UV push button located on the rear right corner of the sample platen Fig 4 3 VersaDoc sample platen UV button 3 Orient and slide the sample tray on top of the sample platen so the notch on the tray fits around the UV button on the sample tray To insert and remove the sample tray the door to the enclosure must be fully opened Chemiluminescent Samples For all small chemiluminescence samples 8 x 8 cm or less slide the sample tray onto the guides on top of each epi illumination assembly This allows the sample to be placed closer to the camera For large chemi samples place the sample tray on the platen per step 3 above to get a wider field of view 4 From the VersaDoc acquisition window select the POSITION function in Step I Figure 4 1 The image display window will now present a real time image of the sample in the chamber that refreshes rapidly User s Manual 4 4 5 You may readjust your sample on the tray using the software generated alignment grid to ensure that it is correctly pla
23. CCD element or pixel is a single photo detector capable of detecting light and converting and storing the information in an electronic form A CCD array can be visualized as a periodic grid array of individual CCD elements analogous to buckets of water When the shutter is open photons of light analogous to drops of rain fall into the photo detectors buckets of water When the camera shutter is open and the CCD is exposed to light A thermoelectric cooler TEC that pulls heat away from the CCD The heat is then transferred to the camera body which is cooled by forced air Dark current arises from the creation of electrons generated through the process of thermal emission within the silicon layers comprising the CCD Dark current noise is the square root of the number of dark current electrons The presence of dark current is an additional concern in low light level applications It is important to ensure that dark current noise does not exceed read noise from the signal even when long integration times are used Signal to noise ratio SNR is the measure of the signal quality at a given pixel It is the ratio of the measured signal to the overall measured noise at that pixel Dynamic range of a CCD is simply defined as the ratio of CCD saturation to the read noise It is the ability to quantitatively detect very dim and very bright pixels within a single image Quantum efficiency is the measure of the effectiveness of an imaging d
24. Manual 6 2 Bad filter wheel cable or Reconnect replace cable connection Bad light Replace light source source Contact Bio Rad Custom lens is causing Contact Bio Rad for obstruction assistance Integration time to acquire image has selected for sample working Service Lamp intensity has Replace light source decreased with age Bad light source reacquire the image Poor chemiluminescence sensitivity Incorrect lens filter used Use the 50 mm high NA lens for best chemi results verify correct application Sample may have Some Chemi substrates degraded have a short lifetime make sure the sample is imaged soon after addition of substrate Sample on platen with Place sample on chemi chemi tray installed tray or remove tray from sample chamber value f 1 4 recommended The 660IR cutoff filter Remove 660 cutoff filter was not removed from in VersaDoc 1000 3000 the lens 4000 5000 only Insufficient integration Integrate for a longer time time Software Troubleshooting In case you installed the card in the system before loading the drivers click on CANCEL when WINDOWS prompts you to point at the drivers for new HARDWARE it has found Follow the steps for installing the software and loading the drivers as shown above and reboot the system to properly install the card and drivers to your MAC or PC as the case may be Should you forget to click on CANCEL when windows prompts you that it has found new HARDWARE It will
25. VersaDoc enclosure can be found within the box With the assistance of a helper remove the VersaDoc from the box Grip the bottom of the enclosure on both sides do not lift the instrument by the front door or electronics area and place it on the floor for the next step in the setup procedure When placing the enclosure allow clear access to the rear panel for connection of the appropriate cables User s Manual 3 2 A A Warning Get a helper a single person should not attempt to lift the VersaDoc Warning To avoid back injury always bend your knees and keep a straight back when lifting heavy objects Caution Do not supply power to the instrument until the VersaDoc system has been set up using the following installation procedures 5 Open all the other boxes and carefully remove all the items 6 Perform a shipping check to confirm that the system has been supplied complete 3 2 2 Shipping Check During the unpacking process inspect all shipping containers to ensure that you have received all ordered items and that no boxes are damaged If items are either missing or damaged this should be noted at the time of installation so that it can be immediately reported to both the shipping company and Bio Rad Technical Support The Quantity One acquisition and analysis software is supplied in a separate box The VersaDoc MP hardware should arrive complete with the following items Quantity 1 VersaDoc light tight enclosure Ver
26. al 5 5 Section 6 Troubleshooting amp Technical Information 6 1 Problem Solving Guide VersaDoc is not found by host computer Power is not supplied or the system is not switched on S Serial USB or AIA cable Reconnect Serial USB AIA is not connected properly cables and ensure it is Camera power cable is not connected properly Ensure power is supplied to the system and that the switch is turned on seated properly Makes sure that the cables are connected and seated properly Change PCI slot to which the digitizing card is connected AIA cable is defective Replace AIA cable Image is not visible on the monitor or only low signal counts are detected Image is not visible on the monitor or only low signal counts are detected Startup sequence is incorrect Computer has a conflicting program or initiation init file a _ The Transform function in the software is set too high Lens cap is covering lens Insufficient integration time Chemi tray not removed when imaging nonchemi sample Wrong application selected Wrong imaging area User s Manual 6 1 Turn off all components and restart in Opposite sequence Contact Bio Rad for assistance Set to a lower maximum value Remove lens cap Integrate for a longer time Remove chemi tray from sample chamber Verify reselect correct application Move sample into imaging area Bad light source Repair light
27. ced and in the center of the viewing area Transillumination Colorimetric samples i e Coomassie Blue stained protein gels and samples requiring UV transillumination i e QDot Blots or white light transillumination i e autoradiographs Do not use the Sample Tray For UV transillumination place the samples directly on the platen Visually check that the sample platen is clean use an optical cleaning solution and a soft lint free towel or lens cleaning tissue to clean it if necessary For samples requiring white light transillumination place the white light conversion screen on the sample platen and place the samples directly on the screen Sample loading in the VersaDoc 1000 3000 4000 and 5000 Instrument Samples should be placed in the VersaDoc instrument following the steps outlined below 1 Open the door 2 Visually check that the sample platen is clean Clean the platen surface using an optical cleaning solution and a soft lint free towel or lens cleaning tissue if needed 3 From the VersaDoc acquisition window select the POSITION function in Step II Figure 4 1 The image display window will now present a real time image of the sample in the chamber that refreshes rapidly 4 Position your sample on the imaging platen using the software generated alignment grid to ensure that it is correctly placed and in the center of the viewing area Note When imaging fluorescent gel samples it is recommended that the sample
28. ct which features and instrument divers you want to install BIO RAD o i z HE Quantity One S E HSK Support seeen Gel Doc EQ ChemiDoc EQ Orive TE Beet ChemiDoc HAS AMersaDoc Driver Ea willbe installed on local hard drive 98 Entire feature will be installed on local hard drive ain uninstalled Feature Description Support drivers for the ChemiD oc sA SWVercaDoc Imaging Devices O Entire feature will be unavailable OKB on your hard drive User s Manual 3 21 4 If you are installing the drivers on local drive select appropriate option by scrolling down as shown in the picture below Click on NEXT 215i x BIO RAD Select Features Please select which features and instrument divers you want to install EE Quantity One es a HSK Support 39 Gel Doc EQ ChemDoc EQ Drive hae ChemDoc HAS VersaLoc Driver Feature Description Support divers for the Chemiloc MASS YersaDoc Imaging Devices Ea Will be installed on local hard drive stalled on the local hard 298KB on your hard drive Brose Wise nstallation wizard Disk Cost Reset lt Back Cancel 5 Drivers will be copied to the appropriate location on your hard drive and at the completion the following window will appear is Quantity One Setup 5 x Quantity One has been successfully installed Click the Finish button to exit this installation a D
29. d left epi assemblies be replaced at the same time To change an epi lamp follow the procedure outlined below Figure 5 3 NOTE The procedure to remove and replace the lamp in the UV or white light epi modules is the same The procedure outlined below applies to both Turn off the VersaDoc and disconnect all power removing the power cable 2 Open the door to the sample compartment Remove ballast cover 3 Find the threaded mounting screw that secures the epi modules to the wall of the enclosure and loosen it to free up the epi module from the wall 4 Disconnect the epi module cables from the ballast assembly and remove the epi module completely from the unit and set it on a clean bench 5 Using a 3 382 hex key remove the three screws that secure the front end plate on the epi modules and slide out the end piece and filter glass and set aside for future use Figure 5 3 Remove the end plate using 3 32 Allenkey Figure 5 3 Removing epi modules User s Manual 5 4 6 This exposes the lamps in the epi module for you to replace To replace the white light lamp or UV lamp simply grab the two metal ends of the old lamp and carefully rotate to release it from the sockets See Figure 5 4 Remove lame qrabbing the two metal ends and turning to release from its socket Figure 5 4 Removing epi module lamp 7 Install the replacement white light lamp by placing its ends in the sockets and rotating until it clicks into
30. details 3 Next connect the CEU to the camera Caution The default voltage setting on the camera power supply module is 120 VAC You must follow the instructions below to make sure proper voltage selection is made and fuse is used for you AC voltage environment Caution The VersaDoc 5000 and MP 5000 cameras module have a BLUE plug installed on the connector on the camera This plug must not be removed until the module is installed to the enclosure and the camera controller unit is ready to be connected to the Camera User s Manual 3 10 Setting the VersaDoc 5000 and MP 5000 System Power Supply Voltage If required 1 Familiarize yourself with the camera electronics unit CEU See Figure 3 6 below ATA Connector on CEU Controller Cable Connector Figure 3 6 VersaDoc MP 5000 and 5000 CEU 2 A small white tab on the AC inlet located on the rear panel indicates the voltage setting on your CEU See Figure 3 7 Insert a small screw driver and pull out the tab White plastic stub shows the current Yoltage setting Make sure it matches with the local voltage Figure 3 7 AC inlet on CEU 3 Pull out the small tab near the AC inlet using a small screwdriver See Figure 3 8 4 The tab holds a fuse Completely remove it This will expose a tiny PCB located in a tiny slot as shown in Figure 3 8 User s Manual 3 11 Small PCB in a tiny slot This side of the PCB has letters on this
31. e your sample 8 Insert the Fluorescent Reference Plate if you are not using a White Light Conversion Screen Close the door Select Continue 10 The system will automatically acquire a reference image and a flat fielded image of the sample will be generated for you to save and analyze For Additional details on Flat Fielding please refer to the Software Manual You may also select the Help button in the acquisition window to access a detailed description of the software functionality Users Manual 4 11 Section 5 Care and Maintenance 5 1 General Maintenance With regular use the VersaDoc MP and VersaDoc Systems should provide years of trouble free operation without any need for regular operator maintenance other than cleaning If you suspect that the VersaDoc System requires servicing please contact your local Bio Rad office The outside surface of the VersaDoc Instrument should be periodically cleaned with water mild liquid soap and a sponge or soft cloth towel Caution Never use abrasive cleaners solvent based cleaners alcohol or scouring pads to clean the external surface of the instrument Caution Always disconnect the VersaDoc Instrument from electrical power prior to cleaning the external surface of the instrument 5 1 1 Cleaning the Sample Stage Area The sample platen and sample stage of the VersaDoc Instrument should be cleaned between imaging sessions to optimize image quality Use powder free gloves when cleani
32. enced None _jAuto Save After Scan Make Backup Copy Save Display _jAuto scale Transform After Acquisition Imaging Area CM Width EEEE Height s 12 4 Software version information can be found by clicking on HELP and then selecting ABOUT QUANTITY ONE in the sub menu User Cancel OK s Manual 6 4 6 3 System Specifications VersaDoc MP System Specifications Blue 468 nm and white light 460 680 nm Red 635 nm optional Green 528 nm optional Light source Epi illumination UV 325 nm and white light 400 750 nm Emission filters Standard filters supplied 530BP28 605BP50 and clear 8 position filter wheel Optional 695BP55 Red Module 640BP35 Transillumination Transillumination area 26 x 25cm Operating Conditions Supply voltage Frequency Operating Temperature Operating Humidity Relative Dimensions Total Weight 100 120 or 220 240 VAC 10 50 60 Hz 10 28 C 21 C recommended lt 0 non condensing 23 W x 26 D x 39 H 96 Ibs VersaDoc System Specifications Light source Transillumination Epi illumination UV 325 nm and white light 400 750 nm UV 340 nm and white light 400 750 nm Standard filters supplied 520LP 530DF70 610LP and clear filters supplied Emission filters 8 position filter wheel Transillumination area 26 x 25cm Operating Conditions Supp
33. entation carefully slide the filter into the open filter wheel position Repeat steps 4 5 for the remaining filters 2 3 4 Replace the filter wheel cover and hand tighten both captive thumbscrews Close the door firmly The filter wheel will automatically reset to the home position and is now ready for operation NOTE The above procedure can also be used for the installation of custom filters Open filter wheel access panel and rotate it downward Unpack and carefully install filter 1 Make sure that filter 1 is all the way in its slot and positioned straight Figure 3 21 User s Manual 3 28 3 2 9 Installing the Lens The VersaDoc system is supplied with two standard lenses a flexible zoom lens and a high NA 50 mm lens with greater collection efficiency Zoom Lens for fluorescence and colorimetric imaging The zoom lens is recommended for use with all fluorescence and colorimetric applications and for high intensity chemiluminescence experiments The zoom capability of this lens supports the imaging of small and large samples The zoom lens is not a high numerical aperture NA lens and should not be used for low intensity chemiluminescence experiments High Numerical Aperture Lens for Chemiluminescence Imaging The Nikon 50 mm high NA f 1 4 lens is designed for optimized light collection efficiency and should be used for all low intensity chemiluminescence experiments The lens can also be used for collecti
34. ers are 1 520LP Long Pass Used for most single color fluorescent stains and labels 2 530BP Band Pass Used for detection of green signal FITC etc in multi color fluorescence experiments 3 610LP Long Pass Used for detection of red signals Texas Red etc in multi color fluorescence experiments 4 Clear Used for system focus and some colorimetric applications To install the filters follow the procedure below for both the VersaDoc and VersaDoc MP Figure 3 21 1 Ensure that all power and communication cables are connected and that the VersaDoc system is turned on User s Manual 3 27 00 Open the door This will expose the filter assembly filter advance button and the lens mount assembly Loosen the left side screw of the filter wheel cover and remove the right side thumbscrew completely by fully unscrewing it The cover can now be rotated downward to expose the filter wheel Push the filter advance button until filter position 1 is in the center front position Advance to filter position 2 on the VersaDoc MP if the red LED module was not purchased The 1 label should be clearly visible on both sides of the filter slot Remove filter 1 from its packaging and check that it is clean free of dust fingerprints and scratches If the filter is dirty it should be cleaned with the materials provided in the lens and filter cleaning kit Holding the filter by the numbered tab with the number in the correct ori
35. evice to produce electronic charge from incident photons This is an especially important property when performing very low light level imaging There are a variety of different grades of CCD chips Each grade has some percentage of dead or bad pixels These are typically displayed as white or dark lines on the image Most CCD systems correct for dead pixels Image resolution refers to the spacing of pixels in the image and is measured in pixels per inch ppi If an image has a resolution of 72 ppi this means that it contains 5182 pixels 72 x 72 in a square inch Monitor resolution defines the number of dots or pixels per unit length of output It is commonly measured in dots per inch dpi The monitor resolution determines the size of the displayed image and should not be confused with image resolution which reflects the spacing of pixels in the image User s Manual 6 8 6 6 Spare Parts List Part Number Description 8000232 EPI WHITE Module Right 8005363 Kit Focusing Target 1002364 You will need these in pairs Other Useful Part Numbers Part Number Description 1708007 VersaDoc Chemi Tray 1708655 VersaDoc MP Sample Tray Camera Module VersaDoc 4000 1707706 1707725 1707726 Tamron Zoom Lens 28 80 mm 1707729 660 NM Short Pass Filter 1707731 1708009 1708601 1708609 TDS Quantity One MAC User s Manual 6 9 User s Manual 6 10 ORDER FORM EMISSION FILTER MOLECULAR IMAGER VERSADOC MP SYSTEMS
36. ew driver Figure 3 16B Users Manual 3 16 Figure 3 16B Coupling back end of LED module to LED assembly 5 Remount the LED assembly inside the VersaDoc instrument and tighten the thumbscrews on the front of the assembly Reconnect the connectors to their labeled positions on the back panel Figure 3 17 Figure 3 17 Remounting LED assembly and reattaching connectors NOTE The plugs for each illumination module are keyed and cannot be interchanged Each plug will snap together only to the appropriate socket 6 Re install the access panel to the ballast panel User s Manual 3 17 3 2 6 2 Installing the Epi modules on the VersaDoc Systems ale Remove the access panel mounted on the ballast panel by unthreading the two thumbscrews Figure 3 18 Thumb screws onthe ballast panel Figure 3 18 Ballast panel thumbscrews 2 If not connected already connect the transilluminator cable to the connector labeled UV TRANSILLUMINATOR located on the ballast panel on interior rear wall of the enclosure 3 To install the epi UV and white modules first install the epi UV module by inserting the two prongs on its end plate to the slots located at the back wall 4 Secure the module using the captive screw by lining up the front side of the module with the hole located in the wall of the instrument Figure 3 19A 5 Now connect the cable from the epi UV module to the appropriate connector on the ballast assembly
37. g technology and a highly efficient optical design the VersaDoc system offers researchers the sensitivity uniformity flexibility and dynamic range they require for the analysis of electrophoretic and microplate samples among others With direct imaging and automated acquisition this system can increase laboratory throughput and eliminate the need for chemiluminescent detection using x ray film Flexible Design The VersaDoc Imaging system has a very flexible design The light tight enclosure can accommodate either of the offered camera modules For example the user has the option to upgrade from the VersaDoc MP 4000 to the VersaDoc 5000MP The VersaDoc MP 4000 and the VersaDoc MP 5000 come with light tight enclosure camera and filter wheel The user has the option to choose illumination modules to fit their needs This allows customization of the system by selecting the type of camera and the type of illumination you need 2 2 System Description Figure 2 1 The VersaDoc MP Imaging System User s Manual 2 1 The VersaDoc combines several key components into a unique powerful and fully integrated image analysis system 1 Cooled CCD Technology The VersaDoc system uses cooled CCD technology for image capture and improves image quality by reducing background noise and enhancing the signal to noise ratio This is particularly important for low light chemiluminescence and fluorescence applications 2 Unique Trans and Epi illu
38. ight conversion screen Replacement of such a broken hd platen or white light conversion screen is not covered by warranty or service contract 3 Depress the red release button on the base of the camera housing This is located to the rear right hand side of the lens 4 Rotate the lens in a clockwise direction to the right to release it from the housing 5 Pull the lens down and remove it from the filter wheel assembly 6 Replace the mount cover If the lens is not being used for some time it is recommended that it be stored in its original packaging nenti Du a UAA TER u Figure 3 24 Steps in lens removal 7 Proceed to section 4 Users Manual 3 31 Section 4 Operating the VersaDoc MP and VersaDoc System 4 1 Starting the VersaDoc MP and VersaDoc System Both the VersaDoc and the camera module must be connected to the computer prior to using the VersaDoc system To turn on the VersaDoc press the power switch located on the right hand side of the rear panel of the instrument The green LED on the front will illuminate indicating that the system is ready and to confirm that power is being supplied and all the internal light modules are functioning properly The start up initialization process takes approximately 30 seconds At this time you can also power up the power supply or CEU if you have a VersaDoc 3000 4000MP 5000 or 5000MP system After this time has elapsed the host computer can be turned on Note If the
39. ine on its mount matches the white mark on the base right hand side of the filter wheel assembly 5 Insert the mount of the lens into the base of the filter wneel assembly and turn the lens counter clockwise to the left until you hear it click The white mark and setting indicators on the lens should now be directly in front of you 6 The lens in now locked into position Figure 3 22 Figure 3 22 Steps in zoom lens installation High Sensitivity 50 mm Lens Installation To correctly install the 50 mm high NA lens follow the procedure outlined below Figure 3 23 1 Remove the lens from its packaging and retain the packaging for future storage 2 Remove the protective cover from the lens mount rear of lens and check that the lens is clean free of dust fingerprints and scratches If the lens is dirty it should be cleaned with the materials provided in the lens and filter cleaning kit 3 Open the VersaDoc door and position the lens so that the white line and dot on its mount matches the white mark on the base of the camera assembly 4 Insert the mount of the lens into the base of the camera assembly and turn the lens clockwise to the left until you hear it click The main white line and lens settings are directly in front of you Figure 3 23 Steps in 50 mm lens installation User s Manual 3 30 5 The lens in now locked into position and the lens cap be removed Lens Removal To remove the lens from the fi
40. ing 695BP filter Fluorescent Blot VersaDoc Blot on transilluminator VersaDoc MP Sample Tray VersaDoc Blot on transilluminator VersaDoc MP Blot on transilluminator Colorimetric Gel 0 1 1sec Zoom R VersaDoc White Light conversion screen f fer VersaDoc MP White Light conversion screen a 1 1 sec VersaDoc Chemi Tray VersaDoc MP Sample Tray ae ae VersaDoc MP White Light conversion screen Chemiluminescence 5 10min 50 mm VersaDoc Chemi Tray if blot is Weak Signal VersaDoc MP if blot is Chemiluminescence 10 sec 2 min 50 mm VersaDoc Chemi Tray if blot is Strong Signal small VersaDoc MP Sample Tray if blot is small IR filter is not required for the VersaDoc MP Systems 4 3 7 Acquiring the Image To collect the sample image simply select the Acquire button The yellow LED on the front panel of the VersaDoc instrument will flash during acquisition to indicate that the image is being captured The software will automatically set instrument parameters for the filter and light source NOTE You will notice that after the initial image capture the software also acquires a dark image This image is taken for the same amount of time as the exposure however this dark image is acquired with the camera shutter closed This provides a very accurate way to measure and remove the dark current noise from the image see Glossary of Imaging Terms for definition of dark current When your applicat
41. io Rad or its representative or that have been used in violation of Bio Rad instructions It also does not extend to instruments or parts thereof that have been used with fittings or other spare parts not authorized by Bio Rad Laboratories that are interfaced to inappropriate external devices that have been exposed to inappropriate solvents cleaning agents or samples The warranty also does not cover instrument damage resulting from facility problems such as power surges The foregoing obligations are in lieu of all other obligations and liabilities including negligence and all warranties of merchantability fitness for a particular purpose otherwise expressed or implied in fact or by law and state Bio Rad s entire and exclusive liability and the buyers exclusive remedy for any claims or damages in connection with the furnishing of goods or parts their design suitability for use installation and operation Bio Rad Laboratories will in no event be held liable for any special incidental or consequential damages whatsoever and Bio Rad s liability under no circumstances will exceed the contract price for the goods for which liability is claimed User s Manual 6 7 6 5 Glossary of Imaging Terms CCD CCD Element CCD Array Integration Thermoelectric Cooler Dark Current Signal to Noise Dynamic Range Quantum Efficiency Dead Pixels Image Resolution Monitor Resolution Charge Coupled Device Each
42. ion requires white light transillumination the software will also prompt you to place the VersaDoc white light conversion screen on the UV transilluminator platen This white light conversion screen absorbs the light from the UV transilluminator and converts this broadband UV into white light See figure 4 7 User s Manual 4 9 Figure 4 7 White Light Conversion Screen Once the image is captured it will be displayed in its own window You may save the captured image for storage and or future manipulation and analysis After the image has been acquired its appearance can be optimized The image may also be analyzed in various ways using the Quantity One program Analysis options include object volume analysis lane profile analysis including regression analysis and molecular weight determination colony counting fingerprinting VNTR and differential display studies The image and various data reports may be printed or exported to other software programs Please refer to the Quantity One software manual for detailed instructions Note Please refer to the Quantity One software manual for information concerning additional features available in the acquisition window for VersaDoc 4 3 8 Acquiring the Image with Flat Fielding Flat fielding is a mathematical image correction that accounts for lens and light source non uniformities For more accurate data quantification this method of image acquisition is recommended To generate a flat field c
43. iscovery Series anzel 6 Click on FINISH and the system will shut down At this time install the PCI card into the system and REBOOT the system 7 As the system re boots and Windows 2000 XP starts it will find the PCI card and go through a FOUND NEW HARDWARE installation routine and find the drivers that were just installed Users Manual 3 22 NOTE In case the PCI card was installed before the software then go to section 6 for software troubleshooting In case of the MAC systems it does not matter if the PCI Card is installed before or after installing the software and drivers Mac based VersaDoc systems NOTE This procedure applies to both Mac OS 9 xx and OS 10 xx based systems 1 Insert The Discovery Series software CD into the CD ROM drive of the MAC Installer will open and offer choices AeA Quantity One Installer Easy Install HH Read Me This will install Quantity One To also install drivers for an imaging device choose Custom Install from the popup menu Install Location l yey _ ar Quit items will be installed in the folder Applications on the jo disk OSX 10 4 Install Location OSH 10 4 B Install 2 Choose custom install from the pulldown menu in this window and click on INSTALL The following window will open Custom Install HH Read Me EJ Quantity One EJ Users Guide O Gel Doc XR Drivers CL Gel Doc EG ChemiDoc EQ Dr
44. it 1 UV Shield Focusing Target Power Cord Instruction Manual Camera Specification File CD 58mm 52mm Step Down Ring Warranty Card User s Manual 3 6 These are provided with VersaDoc 1000 model only These are provided with VersaDoc 3000 4000 and 5000 models NOTE Please retain all packaging materials for future transport of the VersaDoc system Additional charges will be assessed if packaging is not available for instrument warranty shipping 3 2 3 Coupling the Camera Module to the enclosure This applies to all camera modules VersaDoc MP 4000 MP 5000 VersaDoc 1000 3000 4000 and 5000 Follow the procedure outlined below to mount the camera module to the enclosure 1 Leave the enclosure on the floor for easy access to the camera mount 2 Correctly position the camera module on the camera mount located at the top of the enclosure such that the three holes in the camera module adapter plate will match the three standoffs located in the filter wheel assembly in the camera mounting plate NOTE The camera module should sit completely flat and square on the surface of the Enclosure to avoid light leaks 3 Using the 3 cap head screws and the 9 64 hex key included in camera box secure the camera adapter plate to the standoffs Carefully tighten the screws evenly to form proper light seal See figure 3 1 for details Three cap head screws to secure the camera to the filter wheel assembly Figure 3 1
45. ivers O ChemiDar XRS Driwers bJ VersaDoc Drivers O PharosFx FX PMI Driver Disk space available gt 4GB Approximate disk space needed 40 712K install I neatinn i tems will be installed in the folder Applications on the C Quit ose disk OSX 10 4 Install Location OSX 10 4 User s Manual 3 23 3 Depending upon the Imaging device you are installing click on the desired Imager you want to install and then click on Install The drivers will get loaded and at the completion of the installation the following window will open die Installation was successful You have installed software which suggests you restart your computer If you are finished click Restart or click Quit to leave the Installer If you wish to perform additional installations click Continue Continue Quit Restart 4 Click on Restart 5 Please read the Read Me file before using the system Read Me Quantity One pdf 1 page Welcome to Quantity One 4 6 3 Thank you for installing Quantity One 1 D analysis software A hardware security key HSH is included with this version of Quantity One Before continuing with installation be sure the HSK is plugged in to an available USB port Note that the download version of Quantity One has a free 30 day trial penod during which you do not normally need an HSK After the trial period expires you can continue to use Quantity One in Basic mode See the Release Notes and Use
46. keep in mind that Bio Rad offers a range of comprehensive service agreements that can be tailored to meet your specific needs Bio Rad Laboratories is dedicated to your total satisfaction and would be pleased to answer any questions that you may have How to Contact Bio Rad Laboratories In the United States you can reach Bio Rad Laboratories at the following numbers For general information Toll free 1 800 4BIORAD 1 800 424 6723 Fax 1 510 741 5802 email lsg techserv us bio rad com For service or technical assistance Toll free 1 800 424 6723 Fax 1 510 741 5802 For information concerning Bio Rad Laboratories and its products visit our Worldwide Web site at http www bio rad com Table of Contents Section 1 General Information PAGE 2 1 2 3 Power Safety Information 1 4 UV Safety Information Section2 Introduction ae System Capabilities System Description Mechanical Description Overview of the Imaging Process Illumination Flat Fielding Section 3 System Installation Operating Requirements 3 1 2 1 Unpacking 3 2 7 Electrical and Communication Connections 3 19 a a EEE eee Section4 System Operation 1 Starting the VersaDoc 4 1 Placing samples in the VersaDoc MP Instrument 43 Placing samples in the VersaDoc Instrument 45 System Location Acquiring the Image 4 3 8 Acquiring the Image with Flat Fielding AC EE C Te e 5 1 2 Cleaning the Lens _ 599 Replaci
47. lter wheel housing follow the procedure outlined below Figure 3 24 1 Place the lens cap on lens 2 Hold the lens firmly with your left hand throughout the remainder or the removal process so that it cannot be dropped accidentally Caution Accidentally dropping the lens could break the UV transilluminator l platen or white light conversion screen Replacement of such a broken hd platen or white light conversion screen is not covered by warranty or service contract 3 Depress the red release button on the base of the camera housing This is located to the rear right hand side of the lens 4 Rotate the lens in a clockwise direction to the right to release it from the housing 5 Pull the lens down and remove it from the filter wheel assembly 6 Replace the mount cover If the lens is not being used for some time it is recommended that it be stored in its original packaging nenti Du a UAA TER u Figure 3 24 Steps in lens removal 7 Proceed to section 4 Users Manual 3 31 5 The lens in now locked into position and the lens cap be removed Lens Removal To remove the lens from the filter wheel housing follow the procedure outlined below Figure 3 24 1 Place the lens cap on lens 2 Hold the lens firmly with your left hand throughout the remainder or the removal process so that it cannot be dropped accidentally Caution Accidentally dropping the lens could break the UV transilluminator l platen or white l
48. ly voltage Frequency Operating Temperature Operating Humidity Relative Dimensions Total Weight 100 120 or 220 240 VAC 10 50 60 Hz 10 28 C 21 C recommended lt 0 non condensing 23 W x 26 D x 39 H 96 Ibs User s Manual 6 5 VersaDoc 1000 Cooled CCD Specification Imaging array 1317 X 1035 Pixel size 6 8 x 6 8 micron Pixel depth 12bit Detector type Scientific grade high sensitivity front illuminated CCD with antireflective coating Cooling system Forced air peltier thermoelectric system Cooling range 10 C 2 es Dynamic Range 3 4 orders Illumination Flat Fielding Y VersaDoc 3000 Cooled CCD Specification Detector type Scientific grade high sensitivity front illuminated CCD with antireflective coating Cooling range 25 C 2 C VersaDoc 4000 and 4000 MP OF oo o IE 0x 01 D Specification Detector type Scientific grade high sensitivity front illuminated CCD with antireflective coating and micro lenses Cooling range 10 C 2 Dynamic Range 3 4 orders Illumination Flat Fielding Yes VersaDoc 5000 and 5000 MP OF oo o IE O01 D Specification Pixel size 24 x 24 micron Detector type Scientific grade high sensitivity back illuminated CCD with antireflective coating Cooling range 25 to 35 C 2 C 35 MP 5000 Illumination Flat Fielding Yes User s Manual 6 6 6 4 VersaDoc MP and VersaDoc Systems Warranty I
49. mination Source The VersaDoc MP system incorporates unique red green blue and white LED epi illumination and white uv transillumination to cover visible uv and white light excitation The variety of excitation sources in the VersaDoc MP allow excitation of samples multi plexed with 3 fluorescent dyes or nanoparticles The VersaDoc systems have trans and epi illumination modules for both UV and white light excitation These illumination sources provide high sensitivity imaging of a variety of fluorescent chemifluorescent and colorimetric samples The broad bandwidth UV excitation 290 365 nm supports the detection of a broad range of fluorescent dyes 3 Interchangeable Lens The VersaDoc is supplied with two standard lenses One 28 80 mm zoom and one 50 mm mono focal The 28 80 mm zoom is ideal for most fluorescence and colorimetric applications The 50 mm f1 4 fixed lens has a high light collection efficiency and is the lens recommended for all chemiluminescence applications You may purchase an optional 105 mm lens that can be used for imaging small samples at high resolution The VersaDoc will accommodate most Nikon f mount lenses with a minimum operating distance MOD of 0 65 m It is highly recommended that lenses be purchased through Bio Rad as some Nikon lenses require modification before they can be inserted into the filter wheel housing Use of an unmodified lens may result in damage to either the instrument or lens moun
50. n Step Figure 4 1 and identify the application name matching your sample type For detailed acquisition instructions please refer to the Quantity One software instruction manual on the TDS CD 4 3 3 Placing Samples in the Imaging Chamber The VersaDoc MP and VersaDoc Systems can accommodate a variety of sample types and sizes The sample stage is 26 X 25 cm This configuration supports the acquisition of smaller areas of interest within very large samples The imaging area is sealed so wet samples may be placed directly on the UV transilluminator platen With wet samples you will find it convenient to use the sample holders provided with your system These sample holders have suction cups that hold the sample in place and keep it from sliding around on the polished glass surface Caution The sample stage area and the sample trays are resistant to most A research chemicals but may be damaged by extended contact with strong acid or base solutions and organic solvents When imaging samples exposed to these chemicals users should handwash the sample stage and sample trays with water and wipe dry immediately after imaging Sample loading in the VersaDoc 4000MP and VersaDoc 5000MP Instrument Chemiluminescent opaque colorimetric samples blots and fluorescent samples utilizing epi illumination should be placed in the VersaDoc MP instrument using the steps outlined below 1 Use the sample tray Figure 4 2 Visually check that the sample
51. n VesaDoc MP systems 1 Remove the ballast access panel by unthreading the two mounting thumbscrews Figure 3 13 Back panel Thumbscrews Figure 3 13 Ballast panel inside the VersaDoc MP and mounting thumbscews Users Manual 3 14 2 Locate and disconnect the Blue White connector by pressing on the tab and pulling downward Figure 3 14 Figure 3 14 Blue White LED module connector 3 Remove LED assembly Note Secure the LED assembly with one hand throughout this step to prevent it from falling on the sample platen Secure the LED module with hand one and loosen the thumbscrew at the front end of the LED module Figure 3 15A Figure 3 15A LED Assembly thumbscrew And pull the LED assembly outward to slide it off its double pronged groove mount Figure 3 15B User s Manual 3 15 Figure 3 15B LED Assembly groove mount 4 The LED assembly is labeled Red Blue and Green to indicate the mounting positions of the LED modules Slide the LED module in the appropriate location on the assembly Fit the lip on the front end of the LED into the groove on the front end of the LED assembly Use a 3 32 hex allen key to secure the front end of the LED module to the assembly Figure 3 16A Groove mount On LED Assembly Lip mount on LED module Figure 3 16A Coupling front end of LED module to LED assembly Screw the back end of the LED module to the LED assembly module using a 2 Phillips scr
52. nformation This warranty statement may vary outside of the continental United States Please contact you local Bio Rad office for the exact terms of your warranty Bio Rad laboratories warrants to the customer that the VersaDoc system catalog number 1708013 or 1708033 1708143 or 1708053 will be free from defects in material and workmanship and will meet all of the performance specifications for a period of one year from the date of shipment This warranty covers all parts and labor lf any defects should occur during this period Bio Rad Laboratories will either replace or repair the defective parts free of charge For the exact terms of warranty please see the Instrument Warranty Card In the event that the VersaDoc must be returned to the factory for repair under warranty the instrument must be packed and returned in its original shipping container Bio Rad shall not be liable for any incidental special or consequential loss damage or expense directly or indirectly arising from use of the VersaDoc system Bio Rad makes no warranty whatsoever in regard to products or parts furnished by third parties such being subject to the warranty of their respective manufacturers Service under this warranty shall be requested by contacting your nearest Bio Rad office This warranty does not extend to any instruments or parts thereof that have been subject to misuse neglect or accident or that have been modified or serviced by anyone other than B
53. ng Lamps in the Epi illumination Module VersaDoc 54 1000 3000 4000 5000 Instruments Lens and Filter Storage Section6 Troubleshooting amp Technical Information 65 Glossary of Terms _ _ S y O ey 66 Parts List S O o po FilterOrderFom S y O eo Section 1 General Information 1 1 About this Manual This manual provides instructions for installing operating and maintaining the VersaDoc MP and VersaDoc Imaging Systems This manual uses certain conventions to facilitate understanding of the text material and to assist operators in using the VersaDoc system Conventions Left and right sides of the instrument are as viewed from the front operator s position unless otherwise stated Commands that are typed in from the keyboard are referred to aS lt xxxx gt and when you are expected to use the mouse pointer to activate a button it will be referred to as CLICK xxxx When you are expected to click and drag the mouse to a certain item it will be referred to as SELECT xxxx Notes Cautions and Warnings Notes cautions and warnings are used to highlight certain operating procedures and recommendations A note indicates a special procedure an exception to normal operation or something else of specific interest to the reader Notes are preceded by the word Note in italics A caution precedes an operational step that could damage the instrument or destroy data unless the operator takes ce
54. ng the instrument to avoid fingerprints that may appear during imaging Never wear powdered gloves when cleaning the VersaDoc Instrument Clean the sample platen with water mild liquid soap or optical cleaning solution and a soft sponge or lint free optical tissue Cleaning kits are available from Bio Rad and one is supplied with the instrument Caution Never use abrasive cleaners solvent based detergents or scouring pads to clean the platen or sample tray surfaces 5 1 2 Cleaning the Lens To optimize image quality it is recommended that any lens used with the VersaDoc be cleaned before it is installed in the system Both sides of the lens should be cleaned using an optical cleaning solution and optical tissue Avoid touching the glass surfaces of the lens when installing it into the instrument as fingerprints will affect image quality 5 1 3 Cleaning the Emission Filters To optimize image quality it is recommended that the emission filters installed in the VersaDoc system are periodically cleaned Both sides of each emission filter should be cleaned using an optical cleaning solution and optical tissue Avoid touching the glass surfaces of the filter when installing it into the instrument as fingerprint will affect image quality User s Manual 5 1 5 1 4 Cleaning the Epi Illumination Modules Note This pertains to the Blue White Red and Green LED modules in the VersaDoc MP Systems and the UV or white modules in the VersaDoc 1000
55. ng typical fluorescence and colorimetric images The chemiluminescence sample tray may be used to move the sample closer to the camera Infrared Cutoff Filter not used on VersaDoc MP Systems The VersaDoc 1000 3000 4000 and 5000 Systems are shipped with a 660nm IR cut off filter When performing any fluorescence experiments it is required that the 660 nm infrared cut off filter that is supplied with the VersaDoc is installed on the front of collecting lens This filter will block any infrared signal that will be generated by the UV bulbs substantially reducing image background and improving sensitivity This filter is not required for chemiluminescence experiments and should not be used when collecting low intensity chemiluminescence signals as it will reduce the amount of signal collected Zoom Lens Installation To correctly install the zoom lens follow the procedure outlined below Figure 3 22 1 Remove the lens from its packaging and retain the packaging for future storage 2 Remove the front lens cap and install the 58mm 52mm step down ring then the 660 short pass SP filter onto the lens Skip this step for the VersaDoc MP Systems 3 Remove the protective cover from the lens mount rear of lens and check that the lens is clean If the lens is dirty it should be cleaned with the materials provided in the lens and filter cleaning kit User s Manual 3 29 4 Open the VersaDoc door and position the lens so that the white l
56. nstrument is equipped with powerful sources of Red Green and Blue radiation They are capable of Class 2 Laser radiation Although these are controlled via interlock for user protection User of appropriate protective EYE WARE must be used when the interlock is defeated and exposure to open beams of light is possible NOTE Following is a picture of the CAUTION label for the Class 2 laser radiation and it is located at the top of the instrument behind the door CAL TION Z LED RADIATION WHEN OPEN AND LASS INTERLQCK DEFEATED DG NUT STARE INT BEAM How to Contact Bio Rad Laboratories In the United States you can reach Bio Rad Laboratories at the following numbers For general information Tollfree 1 800 4BIORAD 1 800 424 6723 Fax 1 510 741 5802 email Isg techserv us bio rad com For service or technical support Toll free 1 800 424 6723 Fax 1 510 741 5802 For information concerning Bio Rad Laboratories and its products visit our Worldwide Web site at http www bio rad com User s Manual 1 5 Section 2 Introduction 2 1 VersaDoc MP and VersaDoc System Capabilities The VersaDoc MP and VersaDoc are a quantitative imaging systems for capturing high resolution digital images from single and multi color fluorescence chemiluminescence chemifluorescence and colorimetric samples Using cooled CCD technology an exceptionally uniform LED based visible excitation unique ultraviolet illumination mechanism flat fieldin
57. oc system first 1 Also power up the power supply block in case of the VersaDoc 3000 and VersaDoc MP 4000 or CEU in case of the VersaDoc 5000 system 3 Power up the PC Mac second 4 In case of a PC connected to the VersaDoc via USB connection a window will indicate that a new USB device has been found 5 Proceed to the next step to complete the installation Users Manual 3 26 3 2 8 Installing Filters The VersaDoc MP 4000 and VersaDoc MP 5000 are supplied with two emission filters and one clear filter These must be installed in the 8 position filter wheel before use These filters and their corresponding filter wheel positions are 1 695BP Band Pass Optimized for detection of far red fluorescence Optional with Red LED module i e Cy 5 AlexaFluor 647 QDot 705 2 530BP Band Pass Optimized for detection of green fluorescence i e Fluorescein Cy2 Pro Q Emerald AlexaFluor 488 QDot 525 FITC SYBR dyes FAM Picogreen Oligreen Standard emission filter 3 605BP Band Pass Used for detection of red fluorescence i e Cy3 Pro Q Diamond AlexaFluor 555 QDot 605 Flamingo Radiant Red Texas Red SYPRO dyes Used for system focus and some colorimetric 4 Clear oe applications Standard emission filter Standard emission filter The VersaDoc is supplied with three emission filters and one clear filter These must be installed in the 8 position filter wheel before use These filt
58. of ethidium bromide DNAStar SYBR Green SYBR Gold Radiant Red SYPRO Orange SYPRO Red SYPRO Ruby Texas Red Cy2 Cy3 and most fluorescein and rhodamine derivatives Filters 2 530BP60 and 3 610LP are for the independent detection of green fluorescein and red Texas Red fluorescence in multiple colored samples These filters effectively support multiplexing analysis for increased sample throughput and more accurate molecular weight determination Filter 4 is a clear filter that can be used for white light applications Filter positions Filter 6 7 amp 8 are available to users for the installation of application specific custom filters Filter position 5 should be left vacant for the optimized collection of chemiluminescent samples 5 Quantity One 1 D Analysis Software The Quantity One software permits user friendly control of the VersaDoc MP and VersaDoc Imaging Systems and accurate analysis of the captured image or data Quantity One is designed for operation in a Windows or Macintosh environment and supports fully automated application based image acquisition The Quantity One package allows substantial flexibility in the presentation of captured images and provides many tools for data analysis These include molecular weight determination automated lane and band finding accurate concentration analysis VNTR and differential display studies and colony counting Please refer to the Quantity One instruction manual for a f
59. off the epi lights by deselecting the box labeled Lights On see figure 4 1 This will turn off the white lights If the images still show up as saturated red then you must adjust the aperture to a lower setting to view the images in the focus mode Now you may adjust the focusing ring See Figure 4 6 on the lens to achieve a sharp focus on your sample 4 3 6 Exposure Time The exposure time refers to the period of time that the shutter will remain open and light will pass from the sample to the CCD As such the longer the exposure time the brighter the captured image will be For high intensity applications including colorimetric and high intensity fluorescence experiments an exposure time of only a few seconds or less is typically required For low intensity applications such as chemiluminescence an exposure time of several minutes may be required The exposure period required to produce an optimal image varies considerably and may need to be optimized for your particular sample Typical exposure conditions for different sample types have been included in Table 4 1 as a guide to selecting a suitable exposure time This table also indicates the preferred lens for the sample and if any accessories are recommended User s Manual 4 8 Table 4 1 Recommended exposure times and setup VersaDoc Gel on transilluminator Stained VersaDoc MP Sample Tray if using 695BP filter VersaDoc Gel on transilluminator Labeled VersaDoc MP Sample Tray if us
60. orrected image follow the steps below 1 Select the Flat Fielding box in the acquisition window 2 Select Acquire 3 Following the exposure a window will open asking Would you would like to use the saved reference image for Flat Fielding Quantity One Illumination Flat Fielding Do you want to use the sawed reference image for Image Optimization d Yes X No 4 Select No unless you have recently acquired a Flat Field corrected image using the exact same illumination and lens settings of your current sample image 5 Select Yes if you are using the exact same lens and illumination settings as your last image This will apply the previously generated Flat Field reference file to the current image The corrected final image will appear in a new window User s Manual 4 10 6 When No was selected a For fluorescent images you will be prompted to remove your sample and place the Fluorescent Reference Plate in the instrument Do not move the Sample Tray if one was used for the image b For White Light Conversion Screen images you will be prompted to remove your sample from the instrument Do not move the White Light Conversion Screen Quantity One Illumination Flat Fielding Remove your sample and Insert the VersaDoc fluorescent reference plate Continue X Cancel 7 Remove the sample Clean the imaging surface Do not move the Sample Tray or White Light Conversion Screen if one was used to imag
61. ple image is collected and saved this Image can then be reviewed and analyzed using the appropriate tools bundled in the Bio Rad software package Please refer to the software manual for further details User s Manual 2 4 2 5 Illumination Flat Fielding Uniform images are essential for quantitative analysis The VersaDoc Imaging System features a proprietary algorithm that allows elimination of image non uniformities caused by the lens and illumination source Optimization of the image uniformity is made possible via the Illumination Flat Fielding function in the image acquisition portion of the software NOTE This feature is used for all imaging applications on the VersaDoc MP except white light epi illumination It is used only for imaging applications that utilize a UV or white light transillumination source in the VersaDoc lllumination Flat Fielding when using the Sample Tray VersaDoc MP The software takes an initial image of the sample loaded on the sample tray and then prompts the user to remove the sample and to place the VersaDoc Fluorescent Reference Plate provided with your system on the sample platform Illumination Flat Fielding when using the UV transilluminator The software takes an initial image and then prompts the user to remove the sample and to place the VersaDoc Fluorescent Reference Plate provided with your system on the UV Transilluminator illumination Flat Fielding when using the white light conversion sc
62. r Guide for more information This installer also installs Solobug TDS Please use Solobug for reporting any feature requests or design changes See the Release Notes or on line Help for more information User s Manual 3 24 Installing the PCI digitizing card in the PC Caution Ensure that the VersaDoc sytem is turned off before installing the digitizing card and AIA cable 1 Now install the digitizing card into a PCI slot in your computer and secure it with an appropriate screw PCI digitizing card PCI slot inside the PC 2 Connect the camera to the digitizing card with the AIA cable Do not power up the PC Mac yet See below for powering up the computer 3 With both Serial or USB and AIA cables connected your system will appear as shown in one of the configurations below Power Cable E SenallUSB i i ct amp i m AIA Cable Power Supply 7 Figure 3 18d VersaDoc 4000MP and VersaDoc 3000 Systems under Serial xxxBR300 VersaDoc 5000 System starting Serial xxxBR300 User s Manual 3 25 Fan Cable Control Figure 3 184 Figure 3 18b VersaDoc 1000 3000 4000 Systems under Serial VersaDoc 5000 System under Serial xxxBRI00 xXxxBRIOO0 Power On Sequence Normally the VersaDoc system should be switched on for 30 seconds before the host computer is powered up This protocol is required for the computer to recognize the VersaDoc as a peripheral device Power up the VersaD
63. r high intensity chemiluminescence experiments however it is not ideal for this type of application For the best chemiluminescence results it is recommended that the 50 mm lens with no cut off filter be used The 50 mm high NA f 1 4 lens is designed for optimized light collection efficiency and will produce superior images for all low intensity chemiluminescence experiments The lens can also be used for collecting typical fluorescence and colorimetric images however the imaging area is fixed This lens is designed to work in combination with the chemiluminescence sample tray VersaDoc Systems or sample tray VersaDoc MP Systems placing the sample closer to the camera for improved light collection efficiency Infrared Cutoff Filter in the VersaDoc 1000 3000 4000 and 5000 When performing any fluorescence experiments it is recommended that the 660 nm infrared cut off filter that is supplied with the VersaDoc be installed on the front of the lens see figure 4 5 This filter will block any infrared signal that may be generated by the UV bulbs substantially reducing image background and improving sensitivity This filter is not required for chemiluminescence experiments and should not be present when collecting low intensity chemiluminescence signals as it will reduce the amount of signal collected User s Manual 4 6 Figure 4 5 Shortpass filter Aperture Ring meme a TLEVE Pa y Ga Zoom Ring fe i ih Focus Ring
64. rad com Bio Rad Laboratories 6000 Alfred Nobel Drive Hercules CA 94547 P N 10006612 Rev A Model VersaDoc 4000 MP Serial No 603BR1000 For service or technical assistance contact Bio Rad Laboratories Toll free 1 800 424 6723 Fax 1 510 741 5802 Input 100 240V Max Rated Power 350 W Hi Frequency 50 60 Hz Figure 1 1 For easy customer access the instrument serial number information located at two places in your system 1 On the rear of the instrument 2 Inside the main door User s Manual 1 4 1 2 4 UV Safety Information This instrument uses a powerful source of UV radiation and may cause damage to unprotected eyes and skin The VersaDoc provides safety interlocks on both the main door and the sliding UV transilluminator module to protect the user from accidental UV exposure A plastic UV shield is provided for convenience however it may not be adequate to protect you from accidental UV exposure You must use additional personal UV protection like UV protective eyeglasses gowns gloves etc Warning Do not remove the rear access panel when power is supplied to the instrument or defeat the UV safety interlock Attempting to operate the unit with the cover removed may damage the instrument and expose the operator to hazardous voltages Warning Use of controls or adjustments or performance of procedures other than those specified herein may result in exposure to hazardous UV radiation Caution This i
65. reen The software will prompt the user at the beginning of the image acquisition to place the VersaDoc white light conversion screen on the UV transilluminator The sample is placed on the conversion screen an initial image is taken The software then prompts the user to remove the sample from the VersaDoc white light conversion screen and then finishes image acquisition User s Manual 2 5 A Section 3 System Installation 3 1 Operating Requirements 3 1 1 System Location The VersaDoc system should be located in an area that is free of excessive dust or moisture strong magnetic fields or ionizing radiation It is also highly recommended that the ambient temperature be stable and within the range of 10 C to 28 C 21 C is optimal and that the relative humidity not exceed 70 non condensing Warning Care should be taken when lifting and moving the VersaDoc system to avoid personal injury It is recommended that two people one on each side of the instrument lift the VersaDoc enclosure from the bottom The VersaDoc should be placed on a level bench top with a minimum depth of 70cm and a height clearance of 180cm where there is adequate ventilation for the system s cooling fans to operate The system s feet allow enough clearance for easy removal of your hands from underneath the instrument once the system has been placed on the bench In placing the VersaDoc users should also allow for easy access to the main power switch
66. rtain precautions Cautions are located in the main text are preceded by a Caution statement and are accompanied by a Caution Symbol in the left margin A warning precedes an operating procedure that could cause injury to the operator if not followed correctly Warnings are located in the main text are preceded by a Warning statement and are accompanied by a Warning Symbol in the left margin User s Manual 1 1 1 2 Safety Information This instrument is meant for Laboratory use only and should not be used in any other way or manner that is not specified in this Manual Your safety and the safety of others are very important to us To help you make informed decisions about safety we have provided comprehensive operating procedures and safety information in this manual and on labels affixed to instrumentation This information will alert you to any potential hazards It is the user s responsibility to take time and read and understand the Safety Information and put it to the best use for a safe operation of the system 1 2 1 General Cautions Caution Ensure that all of the systems ventilation openings are free of interference Excessive heat build up in the instrument may effect performance or cause operational failure Caution With the exception of cleaning or replacing light bulbs refer all servicing to qualified Bio Rad personnel or their agents If you experience technical difficulties with the instrument contact Bio
67. saDoc MP 4000 camera module VersaDoc MP 5000 camera module Users Manual 3 3 Sample Tray Camera AIA interface cable USB communication cable Quantity Item Right Epi Blue LED White LED Module Left Epi Blue LED White LED Module e SampleHolderKit M OOS 1 Power Cord 1 Instruction Manual 1 Camera Specification File CD Warranty Card User s Manual 3 4 The following options are available for your VersaDoc MP Pair of Red LED modules includes 695BP55 filter Pair of Green LED modules White Light Conversion Screen 640BP35 filter gt The VersaDoc hardware should arrive complete with the following items einen oo VersaDoc light tight enclosure VersaDoc camera module Shown Model 1000 camera module model 3000 4000 and 5000 are shown later in this section Pair of UV epiilluminator modules VersaDoc transilluminator module already installed inside the light tight enclosure User s Manual 3 5 Pair of White epi illuminator Modules Sample chemi tray Camera power cable VersaDoc 1000 and 4000 only Or Fan assembly power cable VersaDoc 5000 only Camera AIA interface cable USB communication cable Quantity Item Sample Holder Kit VersaDoc Fluorescent Reference Plate PCI Digitizing Card Filters 530DF70 520LP 610LP Clear 660Cutoff Lenses 20 40mm Zoom 50mm Fixed Lenses 28 80mm Zoom 50mm Fixed Lens and Filter Cleaning K
68. source Dirty optics Ensure that platen filter and lens are clean White light Image has Image artifacts Chemi Sample tray was Use the Chemi Sample Image intensity varies across the not used MP S tray on the UV Filter platen image Fluorescent image has spots and reacquire the image Dirty optics Ensure that platen filter and lens are clean Bad light source Replace light source Door is open Ensure door is closed properly Dust or small particles on Clean the platen lens and the sample platen or filters optics Image was taken without gel being removed from glass plate Remove plate from gel sample Thin polyacrylamide samples can be transferred to exposed x ray film and imaged using UV epi mode Install 660 NM filter onto lens in VersaDoc 1000 3000 4000 5000 only Fluorescent image has high background 660 NM filter is not in place Light leak Check for light leaks ensure Camera Module tightly connected Wrong application to Verify reselect correct selected application High fluorescence Use low fluorescence agarose used agarose Auto fluorescence from Remove sample and sample Destain if acquire a control image appropriate was Increase destain insufficient Proper sample tray is not VersaDoc MP sample tray used should be used for applications that use EPI LED as light sources Filter wheel does not turn Obstruction in filter wheel Check for and remove obstruction User s
69. t 4 Emission Filters An eight position emission filter wheel has been incorporated into the optical design of the VersaDoc to permit multi color image discrimination and the detection of many different fluorescent dyes and nanoparticles The VersaDoc MP is supplied with three standard filters and two optional filters Filter 1 695BP55 optional comes with Red LED module is optimized for single color detection of far red fluorescence i e Cy 5 AlexaFluor 647 QDot 705 Filter 2 530BP28 standard is for the independent detection of green fluorescence i e Fluorescein Cy2 Pro Q Emerald AlexaFluor 488 QDot 525 FITC SYBR Green I SYBR Green II SYBR Gold FAM GelStar Oligreen Picogreen and for ChemiFluorescence User s Manual 2 2 Filter 3 605BP50 standard is optimized for the detection of red fluorescence i e Flamingo Radiant Red Texas Red Cy3 Pro Q Diamond AlexaFluor 555 QDot 605 SYPRO Ruby SYPRO Red SYPRO Orange Ethidium Bromide BODIPY TR Propidium lodide R6G Tamra Deep Purple Nano Orange Filter 4 standard is a clear filter that can be used for white light applications Filter positions Filter 5 6 7 amp 8 are available to users for the installation of application specific custom filters An optional 640BP35 filter is available The VersaDoc is supplied with four standard filters Filter 1 S20LP Long Pass is A optimized for single color detection
70. ter is not purchased from Bio Rad systems compatibility is the responsibility of the user Please check with your local Bio Rad office regarding compatibility for your specific brand of computer 3 2 System Setup There are 3 main phases in the installation of the VersaDoc MP and VersaDoc systems it 2 3 OS OL eS The components are delivered to your laboratory The system is installed by a trained Bio Rad representative Users are trained on the operation of the VersaDoc and accompanying peripherals and software by a Bio Rad representative The following steps for setup of the VersaDoc requires approximately two hours Prepare the VersaDoc hardware Unpack components Perform shipping check Install the camera module Install the transilluminator module and epi illumination modules Connect system cables Install emission filters Install lens Connect electrical and host computer communication cables Power up the instrument Each of these steps is detailed in the following sections 3 2 1 Unpacking the VersaDoc System Components With the exception of operating software VersaDoc components are shipped in pallet supported boxes Unpack the components by following the steps listed below le 2 3 4 Cut the two steel straps supporting the main instrument package Remove the top cardboard lid Remove other packaging materials and secondary boxes from the top Slide the outer sleeve off the box vertically The
71. uisition Window After starting your computer open the Quantity One acquisition and analysis program by double clicking on the Quantity One icon From the FILE menu select VERSADOC to open the instruments acquisition window The following window will open on the screen Note If the computer cannot establish communications with the VersaDoc a message will indicate this and give the user the option of entering a simulation mode Quantity One 4 6 3 Ale Edit wiew Image Lane Band Match Yolume Analysis hepots Window Help iv Pty ate LEL CECEN RRR ORES DERA SRE eer tole me Perl ae oP uF E3 PHE FE E eo e 1n A 3 HF E ii DE E Ea VersaDoc Shep l Select Sppicatian uf Chanrel Channel 2 Channel Channel 4 Selact DIGZ Oy BS56P Red LED 1x ain 1x1 Bin Step Il Positicn Focus Position P ghon Alignment Grid f Focus Step Ill Set Exposure Time ser E t Orrevew Flat Fielding Qroaiire Optimize Exposure a Stoo Display Option _JHighligat Saturated ives Image lle sze 0 SA MIF Optiorr Heb Exposure Status Figure 4 1 VersaDoc acquisition screen in Quantity One User s Manual 4 2 4 3 2 Selecting the Application The Quantity One program uses an application oriented format to simplify user selection of imaging parameters To correctly set the parameters for imaging simply click the Select button i
72. ull description of this software package 2 3 Mechanical Description The VersaDoc MP and VersaDoc Imaging Systems are modular system consisting of the following main hardware components See figure 2 2 1 The light tight enclosure which integrates the sliding UV transillumination module two epi illumination modules sample tray VersaDoc MP or chemiluminescence tray VersaDoc flat fielding reference plate uv shield access to the filter wheel lenses and all the electronics needed for controlling the lights and filterwheel 2 The camera module which integrates the cooled CCD camera Users Manual 2 3 Components of the VersaDoc system Light Tight Enclosure Camera Module Figure 2 2 2 4 Overview of the Imaging Process The acquisition and analysis of image data using VersaDoc technology is a simple five part process Step 1 Samples to be imaged are placed on the transilluminator module or sample tray Step 2 The appropriate imaging method is selected in the VersaDoc acquisition window of Quantity One Step 3 The sample is aligned using the Alignment Grid positioning template in the Quantity One acquisition window Step 4 If required the imaging lens aperture zoom and focus controls are adjusted to obtain the highest quality image Step 5 The desired collection time is selected and the image is captured In cases where Flat Fielding is selected a reference image is also acquired see below Once the sam
73. vement and by pulling them out of the socket Figure 5 2 oe ey din P Remove hex standoffs X4 Remove the baffle exposing the lamp Hold at the metal ends of the lamps and turn to remove Figure 5 2 Removing UV lamps Install the replacement lamp by inserting its ends in the sockets and rotating until it clicks into place Now reinstall the baffles on the lamps by making sure that they are seated correctly Reinstall the cover on the transilluminator module Replace the screws Carefully reinstall the transilluminator assembly back onto the slides by carefully lining up the slides into the guides and then gently push in all the way User s Manual 5 3 NOTE There is no need to push down or up on the levers they are only used to remove the transilluminator assembly 13 Next carefully connect the UV transilluminator cable back to the ballast assembly Turn the power ON to the system 14 Install ballast cover Close the door 15 Try an application that requires use of UV transilluminator to make sure that the new lamps are fully operational 5 2 2 Replacing lamps in the Epi illumination module on the VersaDoc 1000 3000 4000 and 5000 Systems Tools required You will need a size 3 32 hex key to change the lamps in the epi illumination module The VersaDoc has two epi illumination modules each contain single white illumination source and broad bandwidth UV sources It is recommended that lamps in both the right an
74. you have purchased Exercise caution when lifting the instrument It is recommended that at least two persons lift the instrument Lift the instrument by the bottom plate Never lift the instrument by opening at the top which is the plastic camera mounting plate because this will cause light leaks and damage the camera mounting plate User s Manual 1 3 1 2 3 Power Safety Information The VersaDoc Imaging System contains high voltage circuits The user must disconnect the power cord prior to opening the rear access panel or removing the lamp modules for bulb replacement WARNING It is mandatory for the users to power down the system and disconnect the AC mains from the unit before performing any disassembly or repair to the instrument The VersaDoc system is designed and certified to meet EN61010 Safety and EN61326 A1 Electromagnetic Compliance requirements which are internationally accepted safety standards Certified products are safe to use when operated in accordance with the instruction manual This safety certification does not extend to uncertified equipment or accessories even when connected to the VersaDoc system Figure 1 1 shows the serial number certification label which is found on the rear panel of the VersaDoc system This label provides manufacturing data and safety compliance information about the instrument BIO Made in USA 4 K Dat Model No VersaDoc 4000 MP ree Es Serial No 603BR1000 www bio

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