The Leica RM2125 Microtome - Pdfstream.manualsonline.com
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1. The Leica RM2125 Microtome Online Troubleshooting Guide Seica MICROSYSTEMS Leica RM2125 Troubleshooting Guide Table of Contents of Contents Problem Page 1 Thick and thin sections 1 2 2 Compressed sections sections are extremely compressed wrinkled or jammed together 3 4 3 Chatter 5 6 4 Scratches or splits in sections 7 5 No specimen advance no section produced 8 6 Drifting of handwheel 9 7 Specimen is picked up in the return stroke of the specimen arm 10 8 Sections sticking to pressure plate 11 9 Unable to retract specimen 12 10 Microtome makes clunking noise when sectioning 13 Leica RM2125 Troubleshooting Guide Problem 1 Thick and thin sections a Partial sections b Shorter sections in ribbon c Section thickness varies from one section to another d Skipping sections e Varied thickness in one individual section Cause s Corrective Action I lI lil IV V The clearance angle is too small a Systematically try several clearance angle adjustments until the optimum angle is found For the RM2100 series use 2 5 degrees Disposable Insufficient specimen clamping Orientation head Cassette clamp b Check if all the levers are locked and the screws are tightened on the specimen Retighten the levers and screws if necessary c If the clamp lever can be tightened at more than a 60 degree turn adjustment is needed d Schedule a service call for the adjustme2. Undulations Cause s Corrective Action lI Sectioning speed too high a Rotate the handwheel at a slower speed Il Clearance angle too big c Clearance angle adjustment Systematically decrease the clearance angle until the optimum adjustment is obtained h 3 Ill Damage to pressure plate exists Bent Nicked c Replace the pressure plate Part number RM2100 series complete 14050233128 IV Insufficient clamping of specimen and or knife e Check if all the levers are locked and screws are tightened on the specimen knife holder systems Retighten the levers and screws if necessary V Incompatible blades Blade is possibly too thin causing flex if the specimen is too hard e Try using a different brand or grade of blades VI High profile knife may be too thick f Try using a different brand or grade of blades VII Wax buildup on the back of the front pressure plate g Clean back of pressure plate VIII Application issue not a microtome problem h Infiltration Dehydration continued on page 6 back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 3 Chatter continued a Venetian Blind affect b Wash board c Undulations Cause s Corrective Action Decalcification Paraffin is too old Paraffin impurities IX Tabletop stability k Move the microtome or table to a more stable location or replace the table if the table is flimsy X Vibration in spe
3. cimen advance system I Check if the orientation has been changed or disassembled Re install all parts if necessary back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 4 Scratches or splits in sections Cause s Corrective Action I Il Knife Defect in knife edge Hard specimen in block Paraffin on front or back of knife edge Possible staple left in paraffin a Move to a new area of the knife and observe if the scratches move with it If so replace the knife or have the knife sharpened b Notify supervisor about hard specimen in block and how to proceed from there c Clean the knife with a swab moistened with Xylene Stroke the swab in an upward motion away from the blade edge not down on the blade edge d Try to move the blade or replace it TIP Use a wooden stick and run it across the blade once and check again e Clean the pressure plate with a swab moistened in Xylene Blades Defect in knife edge Hard specimen in block Paraffin on front or back of knife edge Coating deterioration on blade edge a Move to a new area of the knife and observe if the scratches move with it If so replace the knife or have the knife sharpened b Notify supervisor about hard specimen in block and how to proceed from there c Clean the knife with a swab moistened with Xylene Stroke the swab in an upward motion away from the blade edge not down on the blade edge d Try to mov
4. e the blade or replace it e Remove paraffin from the front or back of the pressure plate f Clean the pressure plate with a swab moistened in Xylene back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 5 No specimen advance no section produced Cause s Corrective Action I Specimen reached the front feed limit a Turn the coarse feed wheel in the appropriate direction to move the specimen towards the rear limit 3 Il User may accidently be holding the coarse feed wheel while sectioning Ill The coarse feed wheel cannot rotate freely b Remove any obstruction IV Internal mechanism gear may be faulty c Schedule a service call for replacement of the coarse advance mechanism back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 6 Drifting of handwheel Cause s Corrective Action 1 Hand wheel is not balanced properly Handwheel should remain at 12 o clock when positioned at that location No drifting should occur a Adjust the handwheel position and or collar sleeve position Service call is needed back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 7 Specimen is picked up in the return stroke of the specimen arm Cause s Corrective Action I Static electricity charge may be built up on the knife holder or specimen head a Adjust the temperature or humidity level in the room b Use an ionizer to neutralize the static char
5. ection Cause s Corrective Action VI Backlash in specimen advance system I Test for backlash by trying to rotate the object head clockwise and counter clockwise If there is play in the system schedule a service call for the internal adjustments of the specimen advance system t2 back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 2 Compressed sections sections are extremely compressed wrinkled or jammed together a Compressed b Wrinkled c Jammed Cause s Corrective Action 1 Dull knife blade a Use a different part of the cutting edge or use a new knife blade Il Blade knife is gummed up with paraffin b Clean knife with a swab moistened with Xylene Stroke the swab in an upward motion away from the blade edge not down on the blade edge Ill Specimen is too warm c Pre cool the specimen on a cold plate d Cool the specimen in iced water or with an icecube e Use freeze spray immediately before sectioning NOTE Some labs prohibit the use of this spray please advise IV Clearance angle is too big f Clearance angle adjustment Systematically decrease the clearance angle until the optimum adjustment is obtained bs p g Check the leaf spring screw for washers and tightness 0 V Leaf spring is worn h Replace the leaf spring Part number 14050229557 i If the new leaf spring does not solve the problem then replace the locking lever A service call is required f
6. ge c Clean components of the microtome with alcohol back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 8 Sections sticking to pressure plate Cause s Corrective Action 1 Too much paraffin a Clean the knife with a swab moistened with Xylene Stroke the swab in an upward motion away from the blade edge not down on the blade edge Il Dirty pressure plate b Clean the plate with a swab moistened with Xylene Ill Static c Spray static solution Increase humidity Ground the instrument IV Too much Xylene on pressure plate d Remove Xylene with alcohol back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 9 Unable to retract specimen Note This is only on the Leica RM2125 RT model Cause s Corrective Action 1 Retraction mechanism on specimen advance system is defective a Schedule a service call back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 10 Microtome makes clunking noise when sectioning Cause s Corrective Action I Linear bearings in cage are at their lower limits a Linear bearings need to be cleaned and possibly re greased Il Alignment of bearing cage is incorrect b Schedule a service call back to Table of Contents
7. nt of the specimen head clamp lever e Check 4 screws that hold the orientation head to the advance arm f On the cassette clamp check the 4 screws that hold the cassette clamp to the orientation head g Check for movement of the cassette while in the holder If movement exists it may be caused by weak broken springs wax buildup or lack of grease A service call is required to replace spring and properly grease f there is wax build up instruct customer to clean with a sparing amount of Xylene Locking levers are not well lubricated or cleaned which causes them to be not completely fastened or stuck in a non locked position h Remove all locking levers clean off all paraffin and debris then apply a sparing amount of the provided Leica lubricant Lubricate only with recommended KuBer Topas Grease which can be ordered through Leica Part Number 14033624744 Leaf spring is worn i Replace leaf spring Part number 14050229557 j If the new leaf spring does not solve the problem then replace the locking lever A service call is required for this Dull knife blade k Use a different part of the cutting edge or use a new knife blade continued on page 2 back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 1 Thick and thin sections continued a Partial sections b Shorter sections in ribbon c Section thickness varies from one section to another d Skipping sections e Varied thickness in one individual s
8. or this j Check angle of pressure plate facet to see if correct Refer to User Manual page 29 30 VI Paraffin buildup on the back of the pressure plate k Clean knife with a swab moistened with Xylene Stroke the swab in an upward motion away from the blade edge not down on the blade edge VII Knife blade is not parallel with pressure plate continued on page 4 Pressure Plate back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 2 Compressed sections sections are extremely compressed wrinkled or jammed together continued a Compressed b Wrinkled c Jammed Cause s Corrective Action VII Knife blade is not parallel with pressure plate cont l Adjust Pressure Plate with set screws at the bottom of the knife holder turn counter clockwise or clockwise until the blade is parallel with the top of the pressure plate Pressure Plate m When the blade is parallel with the pressure plate the base line of the blade should line up with the top of the pressure plate after being clamped Blade Edge os Pressure Plate VIII Insufficient tension of handwheel n While manually turning rotate the handwheel 5 times and release your hand from the handwheel If the handwheel rotates freely more than 3 times adjustment is needed Schedule a service call back to Table of Contents Leica RM2125 Troubleshooting Guide Problem 3 Chatter a Venetian Blind affect b Wash board c
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