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G1310-90016 - Agilent Technologies

1. Next Steps 9 Ifa standard seal has been installed run the seal wear in procedure see Seal Wear in Procedure on page 160 which includes a replacement of the purge valve frit 10 For the normal phase seal the purge valve frit should be replaced see Exchanging the Purge Valve Frit on page 147 1260 Infinity lsoPump QuatPump User Manual 153 9 Maintenance Maintenance of a Pump Head with Seal Wash Option Tools required p n Description 8710 2392 Hex key 4 mm15 cm long T handle Parts required p n Description 1 01018 23702 Insert tool 1 0905 1175 Wash seal PTFE 1 5062 2484 Gasket seal wash pack of 6 1 5063 6589 Piston seal PTFE carbon filled black pack of 2 default OR 1 0905 1420 PE seals pack of 2 1 5063 6586 Sapphire piston For a complete list of pump head parts please see Pump Head Assembly with Seal Wash Option on page 174 Preparations Switch off pump at the main power switch Remove the front cover Use an optional solvent shutoff valve or lift up solvent filters for avoiding leakages Remove the pump head see Removing the Pump Head Assembly on page 149 Remove the wash solvent tubings from the support ring inlet and outlet 154 1260 Infinity lsoPump QuatPump User Manual Maintenance 9 1 Remove the seal holder and the seal wash support rings from the piston housing Remove the seal holder from the support ring assembly 2 Check the piston surface and r
2. 1260 Infinity lsoPump QuatPump User Manual Maintenance 9 Exchanging the Multi Channel Gradient Valve MCGV Quaternary pump only Tools required p n Description 8710 0899 Screwdriver Pozidriv 1 Parts required p n Description G1311 67701 Multi channel gradient valve MCGV Preparations Switch off pump at the main power switch Remove the front cover Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages The life time of the multi channel gradient valve can be increased by regularly flushing the valve especially when using buffers If using buffers flush all channels of the valve with water to prevent precipitation of the buffer otherwise salt crystals could drop into an unused channel and form plugs that may cause leaks of that channel Such leaks will interfere with the general performance of the valve When using buffers in combination with organic solvents in the Agilent 1260 Infinity Quaternary Pump it is recommended to connect the aequous solutions buffers to one of the bottom ports A and D and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the buffer channel e g A buffer B organic solvent 1260 Infinity IsoPump QuatPump User Manual 161 9 Maintenance Exchanging the Multi Channel Gradient Valve MCGV 1 Disconnect the connecting tube waste tube and the solvent tubes from the
3. Agilent Technologies Notices Agilent Technologies Inc 2010 2012 2013 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61310 90016 Rev B Edition 11 2013 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a s
4. 1260 Infinity IsoPump QuatPump User Manual 10 Parts for Maintenance Pump Head Assembly Without Seal Wash 1 F 8 Figure 17 Pump head assembly without seal wash option 1260 Infinity lsoPump QuatPump User Manual 173 10 Parts for Maintenance Pump Head Assembly with Seal Wash Option Item p n Description G1312 60045 Pump head assembly with seal wash 1 5063 6586 Sapphire piston 2 G1311 60002 Piston housing 3 01018 60027 Support ring seal wash 4 0905 1175 Wash seal PTFE OR 0905 1718 Wash Seal PE 5 5062 2484 Gasket seal wash pack of 6 6 5042 8952 Seal holder 7 5063 6589 Piston seal PTFE carbon filled black pack of 2 default OR 0905 1420 PE seals pack of 2 8 G1311 25200 Pump chamber housing 9 G1312 60066 Passive inlet valve 1220 1260 G1312 60025 Active inlet valve body without cartridge OPTIONAL G1312 60020 Cartridge for active inlet valve 600 bar OPTIONAL 10 G1312 60067 Outlet valve 1220 1260 11 5042 1303 Lock screw 12 G1312 60061 Purge valve 1260 13 0515 2118 Pump head screw M5 60 mm G1398A Active Seal Wash Option Upgrade includes service 14 5067 4793 Peristaltic Pump with Fixation Springs 5065 9978 Tubing 1 mm i d 3 mm o d silicone 5 m for seal wash option 01018 23702 Insert tool 174 1260 Infinity lsoPump QuatPump User Manual Parts for Maintenance 10 Pump Head Assembly with Seal Wash Option The Pump head assembly with seal wash G1312 60045 includes items 1 8 11 and 13 Figure 1
5. G1311 60006 Description Outlet capillary pump to injector Outlet capillary pump to thermostattable autosampler Bottle head assembly Kit of 4 solvent tubes including labels for connection degasser to MCGV Capillary damper to inlet pump chamber 2 Capillary outlet valve 1 to damper Connecting tube MCGV to PIV Connecting tube MCGV to AIV Waste tube 5 m reorder pack PEEK adapter 1 4 28 to 10 32 Adapter AIV to solvent inlet tubes Inline filter OPTIONAL 1260 Infinity IsoPump QuatPump User Manual Parts for Maintenance 10 Hydraulic Path of the Quaternary Pump i We 2 Figure 24 Hydraulic Flow Path of the Quaternary Pump 185 1260 Infinity lsoPump QuatPump User Manual 10 Parts for Maintenance Hydraulic Path of the Isocratic Pump Hydraulic Path of the Isocratic Pump Item p n 1 G1311 81600 2 G1311 81601 G1311 60003 3 G1312 67305 G1329 87300 4 5062 2461 0100 1847 Description Capillary damper to inlet pump chamber 2 Capillary outlet valve 1 to damper Bottle head assembly Outlet capillary pump to injector Outlet capillary pump to thermostattable autosampler Waste tube 5 m reorder pack PEEK adapter 1 4 28 to 10 32 OPTIONAL Adapter AIV to solvent inlet tubes OPTIONAL a 1 So oj b 2 3 4 ai m l Figure 25 Hydraulic flow path of the isocratic pump 186 1260 Infinity l
6. 158 F lt 95 rh at 40 C 104 F Up to 2000 m 6562 ft Up to 4600 m 15091 ft Installation category II Pollution degree 2 Non condensing For storing the module For indoor use only 1260 Infinity lsoPump QuatPump User Manual 25 2 Site Requirements and Specifications Physical Specifications Quaternary Pump Table 2 Physical Specifications Type Specification Comments Weight 14 5 kg 32 Ibs Dimensions 180 x 345 x 435 mm height x width x depth 7 0 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 180 VA 110W 375 BTU Maximum Ambient operating 4 55 C 39 131 F temperature Ambient non operating 40 70 C 40 158 F temperature Humidity lt 95 r h at 40 C 104 F Non condensing Operating altitude Up to 2000 m 6562 ft Non operating altitude Up to 4600 m 15091 ft For storing the module Safety standards Installation category II Pollution degree2 For indoor use only IEC CSA UL 26 1260 Infinity lsoPump QuatPump User Manual Site Requirements and Specifications 2 Performance Specifications Performance Specifications Agilent 1260 Infinity lsocratic Pump G1310B Table 3 Performance Specification Agilent 1260 Infinity Isocratic Pump G1310B Type Hydraulic system Setable flow range Flow range Flow precision Flow accuracy Pressure operating
7. 5 Optimizing Performance Using the Degasser 84 Operational Hints for the Multi Channel Gradient Valve MCGV 85 When to use the Seal Wash Function 86 Choosing the Right Pump Seals 87 Optimize the Compressibility Compensation Setting 88 This chapter gives hints on how to optimize the performance or use additional devices ott Agilent Technologies 83 5 Optimizing Performance Using the Degasser 84 The quaternary pump has a built in degasser which should always be included to the flow path The isocratic pump does not necessarily require the use of an external degasser but for the following conditions the vacuum degasser is recommended e if your detector is used with maximum sensitivity in the low UV wavelength range e if your application requires highest injection precision or e if your application requires highest retention time reproducibility mandatory at flow rates below 0 5 mL min 1260 Infinity lsoPump QuatPump User Manual Optimizing Performance 5 Operational Hints for the Multi Channel Gradient Valve MCGV In a mixture of salt solutions and organic solvent the salt solution might be well dissolved in the organic solvent without showing precipitations However in the mixing point of the gradient valve at the boundary between the two solvents micro precipitation is possible Gravity forces the salt particles to fall down Normally the A channel of the valve is used for the aqueous salt solutio
8. Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters
9. Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1260 Infinity IsoPump QuatPump User Manual 223 12 Hardware Information Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 224 1260 Infinity lsoPump QuatPump User Manu
10. Error ID 2204 2214 The encoder index position in the module is out of adjustment During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the time to reach the index position is too long the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative Index Missing Error ID 2205 2215 2505 The encoder index position in the module was not found during initialization During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is not recognized within a defined time the error message is generated Probable cause Suggested actions 1 Disconnected or defective encoder cable Please contact your Agilent service representative 2 Defective pump drive assembly Please contact your Agilent service representative 1260 Infinity IsoPump QuatPump User Manual Error Information 7 Stroke Length Error ID 2206 2216 The distance between the lower piston position and the upper m
11. Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative 107 7 108 Error Information Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 1260 Infinity lsoPump QuatPump User Manual Error Information 7 Fan Failed Error ID 0068 The cooling fan in the module has failed The ha
12. Turn the flow on a A O N Observe if the solvent in the tubing of channel A is advancing towards the pump If not disconnect the solvent tubing from the MCGV attach a syringe with a syringe adapter and pull the liquid through the degasser Reattach the tubing to the MCGV 6 Pump 30 mL isopropanol to remove residual air bubbles 7 Switch to the next solvent channel and repeat steps 5 and 6 until all channels have been purged 8 Turn the flow off and close the purge valve 1260 Infinity lsoPump QuatPump User Manual 55 3 Installing the Pump Regular Priming When When the pumping system has been turned off for a certain time for example overnight air will rediffuse into the solvent channel between the vacuum degasser and the pump If solvents containing volatile components are left in the degasser without flow for a prolonged period there will be a slight loss of the volatile components Preparations Switch the system on The purge tool of the Lab Advisor can be used for automatically purging the pump 1 Open the purge valve of your pump by turning it counterclockwise and set the flow rate to 5 mL min Flush the vacuum degasser and all tubes with at least 10 mL of solvent Repeat step 1 and 2 for the other channel s of the pump Set the required composition and flow rate for your application and close the purge valve 5 Pump for approximately 10 minutes before starting your application 56 1260 Infinity lsoPump Qua
13. but it will damage the yellow PE seals normal phase applications p n 0905 1420 gt Do not run the seal wear in procedure if PE seals are installed in the pumphead 160 Before replacing your solvent by isopropanol or replacing isopropanol by your solvent consider solvent miscibility For example do not directly switch from buffers to isopropanol and vice versa 1 Place a bottle with 100 mL of isopropanol in the solvent cabinet and put a tubing including bottle head assembly into the bottle 2 If an active inlet valve is installed screw the PEEK adapter 1 4 28 to 10 32 0100 1847 to the AIV and connect the inlet tube from the bottle head directly to it 3 Connect the Restriction capillary 5022 2159 to the purge valve Connect its other end to a waste container 4 Open the purge valve and purge the system for 5 min with isopropanol at a flow rate of 2 mL min 5 Close the purge valve and set the flow to a rate adequate to achieve a pressure of 350 bar Pump 15 min at this pressure to wear in the seals The pressure can be monitored using your instrument control software or tool 6 Turn OFF the pump slowly open the purge valve to release the pressure from the system disconnect the restriction capillary and reinstall the bottle with the solvent for your application 7 Rinse your system with the solvent used for your next application 8 Replace the purge valve frit see Exchanging the Purge Valve Frit on page 147
14. impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing
15. 109 7 Error Information Module Error Messages These errors are pump specific Solvent Zero Counter Error ID 2055 2524 Pump firmware version A 02 32 and higher allow to set solvent bottle fillings in the data system If the volume level in the bottle falls below the specified value the error message appears when the feature is configured accordingly Probable cause Suggested actions 1 Volume in bottle below specified volume Refill bottles and reset solvent counters 2 Incorrect setting Make sure the limits are set correctly Pressure Above Upper Limit Error ID 2014 2500 The system pressure has exceeded the upper pressure limit Probable cause Suggested actions 1 Upper pressure limit set too low Ensure the upper pressure limit is set to a value suitable for the analysis 2 Blockage in the flowpath after the Check for blockage in the flowpath The following damper components are particularly subject to blockage inline filter frit needle autosampler seat capillary autosampler sample loop autosampler column frits and capillaries with small internal diameters e g 50 pm ID 3 Defective damper Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative 110 1260 Infinity IsoPump QuatPump User Manual Error Information 7 Pressure Below Lower Limit Error ID 2015 2501 The system pressure has fallen below the lower pressure li
16. 7 Lost CAN Partner Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system 1260 Infinity lsoPump QuatPump User Manual 105 7 106 Error Information Leak Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Loose or leaking purge valve inlet valve or Ensure pump components are seated correctly outlet va
17. Diagnostics User Interfaces Depending on the user interface the available tests vary Some descriptions are only available in the service manual Table 13 Test functions available vs user interface Test Instant Pilot G4208A Agilent Lab Advisor System Pressure Test Yes B 02 11 Yes B 01 04 Leak Rate Test No Yes B 01 04 SP1 1260 Infinity IsoPump QuatPump User Manual Troubleshooting and Diagnostics 6 Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files
18. MCGV eS Solvent tubes Waste tube 2 Press the lower sides of the cover to unclip it Remove the cover 3 Disconnect the MCGV cable unscrew the two screws and remove the valve Screws 4 Place the new MCGV into position Make sure that channel A of the MCGV is put at the bottom right position Tighten the two screws and connect the cable to its connector Screws 162 1260 Infinity IsoPump QuatPump User Manual Maintenance 9 Exchanging the Multi Channel Gradient Valve MCGV 5 Install the MCGV cover 6 Reconnect the waste funnel with the waste tube holder in the top cover Insert waste tube in the holder in the leak pan and clip tube to the MCGV cover Waste tube holder Waste funnel Clip 7 Reconnect the tube from the inlet valve to the middle position of the MCGV Connect solvent tubes for channels A D from the MCGV to the degasser outlets Waste f ES aste funne DENI K MCGV cover Solvent tubes Waste tube 1260 Infinity IsoPump QuatPump User Manual 163 9 Maintenance Exchanging the Optional Interface Board When Board defective Parts required p n Description 1 G1351 68701 Interface board BCD with external contacts and BCD outputs CAUTION Electronic boards are sensitive to electrostatic discharge ESD and should be handled with care so as not to damage them Touching electronic boards and comp
19. Test The System Pressure Test is a quick test designed to determine the pressure tightness of the system i e the high pressure flow path between pump and column After exchanging flow path components e g pump seals or injection seal use this test to verify the system is pressure tight see System Pressure Test on page 127 Leak Rate Test The Leak Rate Test is a diagnostic test designed to determine the pressure tightness of the pump components When a problem with the pump is suspected use this test to help troubleshoot the pump and its pumping performance see Leak Rate Test on page 132 1260 Infinity lsoPump QuatPump User Manual 93 6 Troubleshooting and Diagnostics Status Indicators Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status indicator SS amp g Power supply indicator Figure 14 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 94 1260 Infinity lsoPump QuatPump User Manual Troubleshooting and Diagnostics 6 Module Status Indicator The module status indicator indicates one of six possible module conditions When the status indicator is OFF and power switch light is on the module is
20. The Instrument Utilities is a basic version of the Lab Advisor with limited functionality required for installation use and maintenance No advanced repair troubleshooting and monitoring functionality is included 1260 Infinity lsoPump QuatPump User Manual 97 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 98 1260 Infinity lsoPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual 7 Error Information What Are Error Messages 101 General Error Messages 102 Timeout 102 Shutdown 103 Remote Timeout 104 Lost CAN Partner 105 Leak 106 Leak Sensor Open 107 Leak Sensor Short 107 Compensation Sensor Open 108 Compensation Sensor Short 108 Fan Failed 109 Open Cover 109 Module Error Messages 110 Solvent Zero Counter 110 Pressure Above Upper Limit 110 Pressure Below Lower Limit 111 Pressure Signal Missing 111 Missing Pressure Reading 112 Wrong Pump Configuration 112 MCGV Fuse 113 AIV Fuse 113 Valve Failed MCGV 114 Motor Drive Power 115 Inlet Valve Missing 116 Temperature Out of Range 116 Temperature Limit Exceeded 117 Servo Restart Failed 118 ott Agilent Technologies 99 7 100 Error Information Pump Head Missing 119 Index Limit 119 Index Adjustment 120 Index Missing 120 Stroke Length 121 Initialization Failed 121 Wait Timeout 122 Degasser signal fail 123 Degasser limit notreached 123 This chapter describes the meaning of error messages and provides information on probable c
21. User Manual Hardware Information 12 Firmware Description The firmware of the instrument consists of two independent sections a non instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are e the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are e the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote error handling diagnostic functions or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 1260 Infinity lsoPump QuatPump User Manual 203 12 Hardware Information 204 Firmware Updates Firmware updates can be done using your user interface PC and Firmware Update Tool with local files on the hard disk e Instant Pilot G4208A with files from a USB Flash Disk Agilent Lab Advisor software B 01 03 and above The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for
22. and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1260 Infinity lsoPump QuatPump User Manual Appendix 13 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product g Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1260 Infinity lsoPump QuatPump User Manual 229 13 Appendix Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of exp
23. contact 189 LAN 189 overview 188 remote 188 RS 232 189 CAN cable 197 changing solvents 5 7 cleaning 141 Communication settings RS 232C 220 compensation sensor open 108 compensation sensor short 108 composition precision 29 composition range 29 compressibility compensation 17 27 29 88 condensation 24 configuration one stack 38 connections flow 51 control software 50 50 D damaged parts 33 defect on arrival 32 degasser limit not reached 123 degasser signal fail 123 degasser when to use 84 delay volume 29 delivery checklist 33 dimensions 25 26 dual piston in series design 12 E electrical connections descriptions of 210 electronic waste 229 electrostatic discharge ESD 164 EMF early maintenance feedback 223 1260 Infinity lsoPump QuatPump User Manual error messages degasser limit not reached 123 error messages degasser signal fail 123 error messages AIV fuse 113 compensation sensor open 108 compensation sensor short 108 fan failed 109 ignition without cover 109 109 index adjustment 120 index limit 119 index missing 120 initialization failed 121 inlet valve missing 116 eak sensor open 107 eak sensor short 107 eak 106 ost CAN partner 105 MCGV fuse 113 missing pressure reading 112 motor drive power 115 pressure above upper limit 110 pressure below lower limit 111 pressure signal missing 111 pump error 110 pump head missing 119 remote timeout 104 servo restart failed 118 shutdown 10
24. example 1315AB for the G1315A B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 102 is revision 1 02 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Main FW update Resident System Main System Resident FW Update Figure 26 Firmware Update Mechanism 1260 Infinity IsoPump QuatPump User Manual Hardware Information 12 Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agil
25. inlet valve in the module has drawn excessive current causing the inlet valve electronic fuse to open Probable cause Suggested actions 1 Defective active inlet valve Restart the module If the error message appears again exchange the active inlet valve 2 Defective connection cable front panel to Please contact your Agilent service main board representative 3 Defective main board Please contact your Agilent service representative 1260 Infinity IsoPump QuatPump User Manual 113 7 Error Information Valve Failed MCGV Error ID 2040 Valve 0 Failed valve A Valve 1 Failed valve B Valve 2 Failed valve C Valve 3 Failed valve D One of the valves of the multi channel gradient valve has failed to switch correctly The processor monitors the valve voltage before and after each switching cycle If the voltages are outside expected limits the error message is generated Probable cause Suggested actions 1 Gradient valve disconnected Ensure the gradient valve is connected correctly 2 Connection cable inside instrument not Please contact your Agilent service connected representative 3 Connection cable inside instrument Please contact your Agilent service defective representative 4 Gradient valve defective Exchange the gradient valve 114 1260 Infinity IsoPump QuatPump User Manual Motor Drive Power Error ID 2041 2042 Error Information 7 The current drawn by the pump motor exceeded the maximum li
26. mm Hex key 4mm15 cm long T handle Hex key 1 5 mm straight handle 10 cm Hex key 9 64 inch 15 cm long T handle Wrench open end 5 16 3 8 inch Hex key 3 mm12 cm long Hex key 2 5 mm 15 cm long straight handle Hex key 2 0 mm Screwdriver Torx TX8 Double open end wrench 4 mm Syringe Plastic Adapter syringe solvent tube with fitting 1260 Infinity IsoPump QuatPump User Manual 181 10 Parts for Maintenance Solvent Cabinet Solvent Cabinet Item rn oo a A WO N Figure 22 182 p n 5067 4770 5043 0207 5065 9954 5042 8907 9301 1420 9301 1450 G1311 60003 Description Solvent Cabinet Kit Name plate 1260 Front panel solvent cabinet Leak panel Solvent bottle transparent Solvent bottle amber Bottle head assembly Solvent Cabinet Parts 1260 Infinity IsoPump QuatPump User Manual Parts for Maintenance 10 Bottle Head Assembly Item p n Description G1311 60003 Bottle head assembly 1 5063 6598 Ferrules with lock ring 10 Pk 2 5063 6599 Tube screw 10 Pk 3 Wire marker 4 5062 2483 Solvent tubing 5 m 5 5062 8517 Inlet filter adapter 4 Pk 6 5041 2168 Solvent inlet filter 20 um pore size Figure 23 Bottle Head Assembly Parts 1260 Infinity lsoPump QuatPump User Manual 183 10 Parts for Maintenance Hydraulic Path of the Quaternary Pump 184 Item OR OR p n G1312 67305 G1329 87300 G1311 60003 G1322 67300 G1311 81600 G1311 81601 5067 5378 G1311 67304 5062 2461 0100 1847
27. priming solvent due to its miscibility with nearly all HPLC solvents and its excellent wetting properties Description 1 lsopropanol Connect all modules hydraulically as described in the respective module manuals Fill each solvent bottle with 100 mL isopropanol Switch the system on When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can carry health risks gt Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 54 The purge tool of the LabAdvisor or Instrument Utilities can be used to purge the pump automatically If the pump is not able to aspirate the solvent from the bottles use a syringe to draw the solvent manually through tubing and degasser 1260 Infinity IsoPump QuatPump User Manual Installing the Pump 3 When priming the vacuum degasser with a syringe the solvent is drawn through the degasser tubes very quickly The solvent at the degasser outlet will therefore not be fully degassed Pump for approximately 10 minutes at your desired flow rate before starting an analysis This will allow the vacuum degasser to properly degas the solvent in the degasser tubes Open the purge valve of the pump Set the flow rate to 5 mL min Select channel A
28. pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent type The compressibility compensation is set to a default value and can be changed through the user interface Without a compressibility compensation the following will happen during a stroke of the first piston The pressure in the piston chamber increases and the volume in the chamber will be compressed depending on back pressure and solvent type The volume displaced into the system will be reduced by the compressed volume With a compressibility value set the processor calculates a compensation volume that depends on the back pressure of the system and the selected compressibility This compensation volume will be added to the normal stroke volume and compensates the previously described loss of volume during the delivery stroke of the first piston How Does Variable Stroke Volume Work Due to the compression of the pump chamber volume each piston stroke of the pump will generate a small pressure pulsation influencing the flow stability of the pump The amplitude of the pressure pulsation depends mainly on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes generate pressure pulsations of smaller amplitude than higher stroke volumes at the same flow rate In addition the frequency of the pressure pulsations is higher This decreases the influence of flow pulsations on quantitativ
29. seal will be purged for a specified time Periodic a periodic wash will be applied for a defined period in minutes The option is available only when the pump has seal wash capability The seal wash capability is detected by the module itself If seal wash is installed it is recommended to use it in order to increase the primary seal lifetime Module can be turned on at a specified date time This feature can only be used if the module power switch is turned on 1260 Infinity lsoPump QuatPump User Manual 69 4 Using the Pump Method Parameter Settings The pump method setup parameters are in eight sections gt Flow Solvent s Stoptime e Posttime e Pressure Limits e Timetable Advanced e External Contacts Table9 Method parameters Parameter Limits Description Flow 200 bar 0 0 The flow is the rate of movement of eluent along the column It is 10 00 mL min in important that the flow rate is kept constant to ensure precise retention steps of 0 001 time and peak measurements Variations in flow rate can occur as a result gt 200 bar 0 0 of the partial failure of the pumping system air in the pumping system a 5 00 mL mininsteps change in the mobile phase viscosity or a temperature change of 0 001 Solvent s Isocratic Pump The text box allows you to type a brief description of the solvent Quaternary Pump You can set the percentages of solvents B C and D to any value from 0 throug
30. solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets 1260 Infinity lsoPump QuatPump User Manual 41 3 Installing the Pump Installation Information on Leak and Waste Handling Figure 9 Leak and waste handling overview typical stack configuration as an example 42 1260 Infinity IsoPump QuatPump User Manual Installing the Pump 3 1 Solvent cabinet Leak pan 3 Leak pan s outlet port A leak funnel B and corrugated waste tube C 4 Waste tube of the samp
31. your instrument configuration The Configurable Modules panel contains a list of all modules available for configuration The Selected Modules panel contains the list of configured modules Auto Configuration Under Communication settings select either the Host Name option or the IP address option and enter the appropriate value for the host computer to enable automatic detection of the hardware configuration The system configures the instrument automatically with no further manual configuration necessary The Quaternary Pump configuration parameters are in two sections Communication Options Communication The parameters in this dialog box are detected automatically during autoconfiguration Device name Type ID Serial number Firmware revision Button Connection settings Options e Pressure Unit select the pressure units from the drop down list bar psi or MPa e External contacts board installed This check box is marked to indicate that a BCD external contacts board has been detected during autoconfiguration Seal wash installed This check box is marked to indicate that an optional seal wash has been detected during autoconfiguration Please refer to the online help of your user interface for more detailed information 1260 Infinity lsoPump QuatPump User Manual 65 4 66 Using the Pump The Pump User Interface Dashboard Panel Module Graphic The items in the pump graphic have the followin
32. 1C Quaternary Pump VL G5611A Bio inert Quaternary Pump Minimum firmware revisions A 06 33 This test does not work in emulation mode In case of an emulated module convert to the original type first see Replacing the Module Firmware on page 170 Test Principle A solvent can be chosen from available solvent channels and a maximum target pressure can be defined at which the test will be run Typically this is the maximum pressure specified for the pump The test can be run with any solvent compatible to the pump Before the test the pump is flushed with solvent in order to remove air bubbles as air bubbles are compressed during the test and therefore would appear as leaks Using a degasser is highly recommended 1260 Infinity IsoPump QuatPump User Manual Test Functions and Calibration 8 A pressure piston 2 at rear position piston 1 at rear position piston 2 delivers piston 1 delivers about 100 bar below target pressure gt time Initially the pressure is increased to about 100 bar below the target pressure which has been set for the test Then piston 1 is brought to its rear position An increasing flow is delivered by piston 1 In case of a leak the pressure will drop initially as long as the flow rate delivered by the piston is lower than the leak rate As soon as the flow rate of the piston exceeds the leak rate the measured pressure will increase again Therefore the minimum pr
33. 3 solvent zero counter 110 stroke length 121 temperature limit exceeded 117 temperature out of range 116 timeout 102 valve failed 114 wait timeout 122 wrong pump configuration 112 exchanging active inlet valve cartridge 166 active inlet valve 166 inletvalve 140 multi channel gradient valve MCGV 161 optional interface board 164 outlet valve 145 140 passive inlet valve 143 pistons 140 pump seals 140 purge valve frit 147 140 purge valve 147 140 seal wash cartridge 168 external contact cable 198 external contacts BCD board 206 F fan failed 109 firmware description 203 main system 203 resident system 203 update tool 204 updates 204 170 170 upgade downgrade 170 upgrade downgrade 170 flow accuracy 27 29 flow connections 51 flow precision 27 29 flow range 27 29 frequency range 25 26 G general error messages 102 gradient formation 29 gradient valve 161 highest injection precision 84 hints for successful use 61 62 1260 Infinity IsoPump QuatPump User Manual Index HP JetDirect card 208 humidity 25 26 hydraulic path 184 186 hydraulic system 27 29 l index adjustment 120 index limit 119 index missing 120 initialization failed 121 initialization 16 inlet valve missing 116 installation pump module 45 installation bench space 24 instrument layout 224 interface boards optional 202 interface cables 47 interfaces 212 internet 233 introduction to the pump 10 L LAN cab
34. 32 Analog APG Special optional on board Remote G4227A Flex Cube 2 No No No No No CAN DC OUT for CAN slaves 1 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED K1330B 1 Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with ad ditional G1369C LAN Card The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output 214 1260 Infinity lsoPump QuatPump User Manual Hardware Information 12 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and requ
35. 8 Pump Head with Seal Wash Option 1260 Infinity IsoPump QuatPump User Manual 175 10 Parts for Maintenance Outlet Valve p n Description G1312 60067 Outlet valve 1220 1260 Figure 19 Outlet Valve 176 1260 Infinity IsoPump QuatPump User Manual Parts for Maintenance 10 Purge Valve Assembly Item p n Description 1 G1312 60061 Purge valve 1260 2 01018 22707 PTFE frits pack of 5 3 5067 4728 Seal cap WHEY 1 ig U Figure 20 Purge Valve Assembly 1260 Infinity lsoPump QuatPump User Manual 177 10 Parts for Maintenance Active Inlet Valve Assembly Item p n Description G5699A Active Inlet Valve Upgrade Kit includes service and the parts listed below 1 G1312 60025 Active inlet valve body without cartridge 2 G1312 60020 Cartridge for active inlet valve 600 bar G1311 67304 Connecting tube MCGV to AIV needed for quaternary pump only 0100 2298 Adapter PEEK int 1 4 28 to ext 10 32 needed for isocratic pump only Figure 21 Active Inlet Valve Assembly 178 1260 Infinity IsoPump QuatPump User Manual Parts for Maintenance 10 HPLC Starter Kit G4201 68707 HPLC Starter Kit incl 0 17 mm i d cap G4201 68707 p n 9301 1420 3x 9301 1450 01018 22707 5182 0716 5182 0717 5063 6507 2x 5041 2168 2x 5065 9939 1260 Infinity lsoPump QuatPump User Manual Description Solvent bottle transparent Solvent bottle amber PTFE frits pack of 5 Screw Cap Vial 2 mL amber glass write on
36. AA Blue 3 BCD 6 40 a ee Yellow 4 BCD 4 10 Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 1260 Infinity lsoPump QuatPump User Manual 195 11 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module an 1 1 BCD 5 20 2 2 BCD7 80 Se 15 oO Z 3 3 BCD 6 40 oO So 4 4 BCD 4 10 s je 2 5 5 BCDO 1 fie e9 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 196 1260 Infinity IsoPump QuatPump User Manual CAN Cable Identifying Cables 11 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 1260 Infinity lsoPump QuatPump User Manual Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection 197 11 Identifying Cables External Contact Cable c50 o o ol 10 6 15 41 One end of this cable provides a 15 pin plug to be connected to Agilent modules interface boar
37. BCD 2 4 8 BCD 1 2 9 Digital ground 10 BCD 11 800 11 BCD 10 400 12 BCD 9 200 13 BCD 8 100 15 5V Low 1260 Infinity lsoPump QuatPump User Manual 207 12 Hardware Information LAN Communication Interface Board The Agilent modules have one optional board slot that allows to add an interface board to the modules Some modules do not have this interface slot Refer to Interfaces on page 212 for details p n Description G1369B or Interface board LAN G1369 60002 OR G1369C or Interface board LAN G1369 60012 One board is required per Agilent 1260 Infinity instrument It is recommended to add the LAN board to the detector with highest data rate For the configuration of the G1369 LAN Communication Interface card refer to its documentation 208 1260 Infinity IsoPump QuatPump User Manual Hardware Information 12 The following cards can be used with the Agilent 1260 Infinity modules Table 17 LAN Boards Type Vendor Supported networks Interface board LAN G1369B Agilent Technologies Fast Ethernet Ethernet 802 3 or G1369 60002 or RJ 45 10 100Base TX Interface board LAN G1369C recommended for re ordering or G1369 60012 LAN Communication Interface Agilent Technologies Fast Ethernet Ethernet 802 3 board G1369A or RJ 45 10 100Base TX G1369 60001 obsolete J4106A Hewlett Packard Ethernet 802 3 RJ 45 10Base T J4105A Hewlett Packard Token Ring 802 5 DB9 RJ 45 10Base T J4100A
38. Hewlett Packard Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX BNC 10Base2 1 These cards may be no longer orderable Minimum firmware of these Hewlett Packard JetDirect cards is A 05 05 Recommended LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection 1260 Infinity lsoPump QuatPump User Manual 209 12 Hardware Information Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems The interface board slot is used for external contacts and BCD bottle number output or LAN connections e The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch e The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your
39. High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent Signal Name Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 1260 Infinity lsoPump QuatPump User Manual 193 11 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL 7 White 1 Digital ground a Ol ou Brown 2 Prepare run Low 00 pes DE KEY Gray 3 Start Low oo o0 S iE Blue 4 Shut down Low og Pink 5 Not oa connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 194 1260 Infinity IsoPump QuatPump User Manual BCD Cables Identifying Cables 11 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 pea Violet 2 BCD 7 80
40. Hints for Successful Use of the Pump 61 Setting up the Pump with the G4208A Instant Pilot 63 Setting up the Pump with the Instrument Control Interface 64 Solvent Information 74 Algae Growth in HPLC Systems 80 Prevent Blocking of Solvent Filters 81 1260 Infinity lsoPump QuatPump User Manual Contents Optimizing Performance 83 Using the Degasser 84 Operational Hints for the Multi Channel Gradient Valve MCGV 85 When to use the Seal Wash Function 86 Choosing the Right Pump Seals 87 Optimize the Compressibility Compensation Setting 88 Troubleshooting and Diagnostics 91 Overview of the Module s Indicators and Test Functions 92 Status Indicators 94 User Interfaces 96 Agilent Lab Advisor Software 97 Error Information 99 What Are Error Messages 101 General Error Messages 102 Module Error Messages 110 Test Functions and Calibration 125 Introduction 126 System Pressure Test 127 Leak Rate Test 132 Maintenance 137 Introduction to Maintenance and Repair 138 Warnings and Cautions 139 Overview of Maintenance and Repair 140 Cleaning the Module 141 Checking and Replacing the Solvent Filter 142 Exchanging the Passive Inlet Valve PIV 143 Exchanging the Outlet Valve 145 Exchanging the Purge Valve Frit 147 Removing the Pump Head Assembly 149 Maintenance of a Pump Head Without Seal Wash Option 151 Maintenance of a Pump Head with Seal Wash Option 154 Reinstalling the Pump Head Assembly 158 1260 Infinity lsoPump Qu
41. OPTIONAL not orderable OPTIONAL Solvent Cabinet Kit OPTIONAL Lab Advisor incl license OPTIONAL Power cord Items identified as optional are additional accessories They are not included in the standard scope of delivery Items identified as not orderable can be downloaded from the Agilent website http www agilent com Either one of the three columns listed will be part of the delivery as ordered 1260 Infinity IsoPump QuatPump User Manual 35 3 36 Installing the Pump Accessory Kit The Accessory Kit G1311 68755 contains the following items p n 5062 2461 5063 6527 5181 1519 G1329 87300 G1312 87303 5042 9967 Description Waste tube 5 m reorder pack Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste CAN cable Agilent module to module 1 m Capillary ST 0 17 mm x 900 mm S S pump to thermostatted autosampler Capillary ST 0 17 mm x 400 mm S S pump to injector Tubing clip set of 5 clips 1260 Infinity IsoPump QuatPump User Manual Installing the Pump Optimizing the Stack Configuration If your module is part of a complete Agilent 1260 Infinity Liquid Chromatograph you can ensure optimum performance by installing the following configurations These configurations optimize the system flow path ensuring minimum delay volume 1260 Infinity lsoPump QuatPump User Manual 3 37 3 Installing the Pump One Stack Configuration Ensure optimum performance by installing th
42. Pump QuatPump User Manual 3 In This Guide 7 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 8 Test Functions and Calibration This chapter describes the tests for the module 9 Maintenance This chapter describes the maintenance of the module 10 Parts for Maintenance This chapter provides information on parts for maintenance 11 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules 12 Hardware Information This chapter describes the pump in more detail on hardware and electronics 13 Appendix This chapter provides addition information on safety legal and web 1260 Infinity lsoPump QuatPump User Manual Contents Contents 1 Introduction 9 Introduction tothe Pump 10 Overview of the Hydraulic Path 12 System Overview 18 2 Site Requirements and Specifications 21 Site Requirements 22 Physical Specifications Isocratic Pump 25 Physical Specifications Quaternary Pump 26 Performance Specifications 27 3 Installing the Pump 31 Unpacking the Pump 32 Optimizing the Stack Configuration 37 Installation Information on Leak and Waste Handling 41 Installing the Pump 45 Connecting Modules and Control Software 48 Flow Connections of the Pump 51 Priming the System 54 4 Usingthe Pump 59 Leak and Waste Handling 60
43. S G1367E HiP ALS K1367E HiP ALS Clinical Ed G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler Yes No G4226A ALS 2 Yes No Yes 1 Yes Yes No CAN DC OUT for CAN slaves Yes Yes No CAN DC OUT for CAN slaves Yes Yes No THERMOSTAT for G1330B K1330B Yes Yes No THERMOSTAT for G1330B K1330B CAN DC OUT for CAN slaves Yes Yes No Yes 212 1260 Infinity lsoPump QuatPump User Manual Hardware Information 12 Table 18 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes K1314F Clinical Ed G4212A B DAD 2 No Yes Yes 1 Yes K4212B DAD Clinical Ed G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 2 Yes K1321B FLD Clinical Ed G1321C FLD G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A C TCC 2 No No Yes No Yes K1316C TCC Clinical Ed G1322A DEG No No No No No Yes AUX K1322A DEG Clinical Ed G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes K4225A DEG Clinical Ed 1260 Infinity lsoPump QuatPump User Manual 213 12 Hardware Information Table 18 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 2
44. TIONAL HPLC Starter Kit incl 0 12 mm i d cap OPTIONAL Bottle head assembly Agilent 1200 Infinity Series User Documentation DVD OPTIONAL not orderable OPTIONAL Solvent Cabinet Kit OPTIONAL Interface board LAN OPTIONAL Lab Advisor incl license OPTIONAL Power cord Items identified as optional are additional accessories They are not included in the standard scope of delivery Items identified as not orderable can be downloaded from the Agilent website http www agilent com Either one of the three columns listed will be part of the delivery as ordered 34 1260 Infinity IsoPump QuatPump User Manual Installing the Pump 3 G1311B Quaternary Pump Delivery Checklist p n G1311B G1311 60003 4x 61311 90300 64203 68708 959961 902 699975 902 883975 902 G4201 68707 G4202 68707 G1369C G4800 64500 5067 4770 M8500A Description Agilent 1260 Infinity Quaternary Pump optionally with active seal wash Bottle head assembly Agilent 1260 Infinity Quaternary LC System Manual and Quick Reference not orderable HPLC System Tool Kit OPTIONAL Column Eclipse Plus C18 4 6 x 100 mm 3 5 pm OPTIONAL Column Poroshell 120 EC C18 4 6 x 50 mm 2 7 um OPTIONAL Column SB C18 4 6 x 150 mm 5 pm OPTIONAL HPLC Starter Kit incl 0 17 mm i d cap OPTIONAL HPLC Starter Kit incl 0 12 mm i d cap OPTIONAL Interface board LAN OPTIONAL Agilent 1200 Infinity Series User Documentation DVD
45. Timeout Pressure not reached Pump channel A did not reach the delivery phase Pump channel B did not reach the delivery phase Pump channel A did not reach the take in phase Pump channel B did not reach the take in phase Solvent volume not delivered within the specified time Probable cause Suggested actions 1 Purge valve open Ensure that purge valve is closed 2 Leak at fittings purge valve active inlet e Ensure pump components are seated valve outlet valve or piston seals correctly If there are still signs of a leak exchange the appropriate seal purge valve active inlet valve outlet valve piston seal Exchange defective capillaries 3 Flow changed after starting test Ensure correct operating condition for the special application in use 4 Defective pump drive assembly Please contact your Agilent service representative 122 1260 Infinity IsoPump QuatPump User Manual Degasser signal fail Error ID 2243 Error Information 7 The pump board gets no or wrong pressure signals from the built in degasser Parameters e 0 ADC failure e 1 ADC cannot convert pressure signal e 2 Measured pressure out of range negative or higher than 1021 hPa Probable cause 1 Degasser board defect missing or not connected to the pump main board 2 Degasser sensor defect or not connected to degasser board 3 If parameter is 0 and the LED on the degasser board blinks it is probably because of a
46. User Manual 75 4 76 Using the Pump Material Information Materials in the flow path are carefully selected based on Agilent s experiences in developing highest quality instruments for HPLC analysis over several decades These materials exhibit excellent robustness under typical HPLC conditions For any special conditions please consult the material information section or contact Agilent Disclaimer Subsequent data were collected from external resources and are meant as a reference Agilent cannot guarantee the correctness and completeness of such information Data is based on compatibility libraries which are not specific for estimating the long term life time under specific but highly variable conditions of UHPLC systems solvents solvent mixtures and samples Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Apart from pure chemical corrosion other effects like electro corrosion electrostatic charging especially for non conductive organic solvents swelling of polymer parts etc need to be considered Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually accelerates at higher temperatures If in doubt please consult technical literature on chemical compatibility of materials PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical r
47. action in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Diamond Like Carbon DLC Diamond Like Carbon is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fused silica and Quartz Si0 Fused silica is used in 1290 Infinity Flow Cells and capillaries Quartz is used for classical flow cell windows It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended 1260 Infinity IsoPump QuatPump User Manual Using the Pump 4 Gold Gold is inert to all commo
48. al 1260 Infinity lsoPump QuatPump User Manual 13 Appendix General Safety Information 226 The Waste Electrical and Electronic Equipment Directive 229 Batteries Information 230 Radio Interference 231 Sound Emission 232 Agilent Technologies on Internet 233 This chapter provides addition information on safety legal and web ee Agilent Technologies 225 13 Appendix General Safety Information Safety Symbols Table 28 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available A and the user should not touch it when heated up WARNING es alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 226 1260 Infinity lsoPump QuatPump User Manual Appendix 13 Gener
49. al Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards 1260 Infinity IsoPump QuatPump User Manual 227 13 Appendix Operation 228 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been
50. at the main power switch Remove the front cover 1 Using a 1 4 inch wrench disconnect the valve capillary from the outlet valve Using the 14 mm wrench loosen the valve and remove it from the pump body Outlet valve f 2 Do not disassemble the outlet valve as this can damage the valve 1260 Infinity IsoPump QuatPump User Manual 145 9 Maintenance Exchanging the Outlet Valve 3 Reinstall the outlet valve and tighten the valve using a torque wrench 12 Nm Reconnect the valve capillary wa NJ 146 1260 Infinity lsoPump QuatPump User Manual Maintenance 9 Exchanging the Purge Valve Frit When Tools required OR Parts required Preparations Frit when piston seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across the frit at a flow rate of 5 mL min of water with purge valve opened Purge valve if internally leaking p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 1924 Wrench open 14 mm Pair of tweezers Toothpick p n Description 1 01018 22707 PTFE frits pack of 5 1 G1312 60061 Purge valve 1260 1 5067 4728 Seal cap OPTIONAL Switch off pump at the main power switch Remove the front cover Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages 1 Using a 1 4 inch wrench disconnect the pump outlet 5 Using a pair of tweezers or a to
51. atPump User Manual Contents Seal Wear in Procedure 160 Exchanging the Multi Channel Gradient Valve MCGV 161 Exchanging the Optional Interface Board 164 Exchanging the Active Inlet Valve AIV or its Cartridge 166 Exchanging the Seal Wash Cartridge 168 Replacing the Module Firmware 170 10 Parts for Maintenance 171 Pump Head Assembly Without Seal Wash 172 Pump Head Assembly with Seal Wash Option 174 Outlet Valve 176 Purge Valve Assembly 177 Active Inlet Valve Assembly 178 HPLC Starter Kit G4201 68707 179 HPLC Starter Kit G4202 68707 180 HPLC System Tool Kit 181 Solvent Cabinet 182 Bottle Head Assembly 183 Hydraulic Path of the Quaternary Pump 184 Hydraulic Path of the Isocratic Pump 186 1 Identifying Cables 187 Cable Overview 188 Analog Cables 190 Remote Cables 192 BCD Cables 195 CAN Cable 197 External Contact Cable 198 Agilent Module to PC 199 Agilent 1200 Module to Printer 200 1260 Infinity IsoPump QuatPump User Manual 7 Contents 12 Hardware Information 201 The Electronics 202 Firmware Description 203 Optional Interface Boards 206 Electrical Connections 210 Interfaces 212 Setting the 8 bit Configuration Switch without On board LAN 219 Early Maintenance Feedback 223 Instrument Layout 224 13 Appendix 225 General Safety Information 226 The Waste Electrical and Electronic Equipment Directive 229 Batteries Information 230 Radio Interference 231 Sound Emission 232 Agilent T
52. auses and suggested actions how to recover from error conditions 1260 Infinity IsoPump QuatPump User Manual Error Information 7 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a metho
53. compressibility settings of water 1 Start the pump with the required flow rate 2 Before starting the optimization procedure the flow must be stable Check the tightness of the system with the pressure test 3 Your pump must be connected to a data system or Instant Pilot with which the pressure and ripple can be monitored or connect an external measurement device to the analog pressure output see Electrical Connections on page 210 4 Starting with a compressibility setting of 40 10 bar increase the value in steps of 10 Re zero the signal display as required The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition If ChemStation classic view is used and the ripple shown is positive then the compressibility setting should be decreased If it is negative it should be increased 1260 Infinity lsoPump QuatPump User Manual Optimizing Performance 5 Table 12 Solvent Compressibility Solvent pure Compressibility 1 10 bar Acetone 126 Acetonitrile 115 Benzene 95 Carbon tetrachloride 110 Chloroform 100 Cyclohexane 118 Ethanol 114 Ethyl acetate 104 Heptane 120 Hexane 150 Isobutanol 100 Isopropanol 100 Methanol 120 1 Propanol 100 Toluene 87 Water 46 1 Please check section Solvent Information for compatibility to your specific LC system 1260 Infinity lsoPump QuatPump User Manual 89 5 Optimizing Performance O
54. current maximum pressure limit Composition A B The contributions of channels A and B to the current solvent composition Composition C D The contributions of channels C and D to the current solvent composition 1260 Infinity IsoPump QuatPump User Manual 67 4 68 Using the Pump Context Menu The context menu of the dashboard panel contains the following commands Ss Control Displays the pump s Control dialog box il S Method Displays the pump s Method Setup dialog box Set Error Method Sets the method that is loaded if an error occurs to the method that is currently available in the hardware Identify Device Causes the LED on the front of the module to blink for a few seconds T Switch Pump On Off Toggles the status of the pump on or off STE Bottle Fillings Displays the Bottle Fillings dialog box 1260 Infinity IsoPump QuatPump User Manual Control Settings Using the Pump The pump control parameters are in three sections Pump Seal Wash e Automatic Turn On Table 8 Pump control parameters 4 Parameter Limits Description Pump Enables you to switch the pump On Off or to a Standby condition In the Standby condition the pump motor is still active and when the pump is switched on again does not need to be re initialized Seal Wash The seal wash can be set up to be run once Single wash or periodically Automatic Turn On Periodic Off no seal wash is used Single wash the
55. d The other end is for general purpose Agilent Module Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 198 1260 Infinity lsoPump QuatPump User Manual Identifying Cables 11 Agilent Module to PC p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m 1260 Infinity lsoPump QuatPump User Manual 199 11 Identifying Cables Agilent 1200 Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control Module 200 1260 Infinity lsoPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual 12 Hardware Information The Electronics 202 Firmware Description 203 Optional Interface Boards 206 Electrical C
56. d assemblies into empty solvent reservoirs and place the bottle in the solvent cabinet Connect the inlet tubes from the bottle head assemblies to the inlet connectors A to D at the right hand side of the vacuum degasser see Figure 13 on page 53 Fix the tubes in the tube clips of the pump Connect the solvent tubes from the MCGV inlet to the outlets of the vacuum degasser Using a piece of sanding paper connect the waste tubing to the purge valve and place it into your waste system If the pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the waste tube to the waste outlet of the pump leak handling system Connect the pump outlet capillary pump to injection device to the outlet of the purge valve Fill solvent reservoirs with your mobile phase 1260 Infinity lsoPump QuatPump User Manual Installing the Pump 3 Flow Connections of the Pump 10 Prime your system before first use see Inital Priming on page 54 Bottle head assembly Tubings Solvent cabinet Purge valve Waste tubing Outlet capillary to autosampler Figure 13 Flow Connections of the Quaternary Pump 1260 Infinity lsoPump QuatPump User Manual 53 3 Installing the Pump Priming the System Inital Priming When Parts required Preparations Before a degasser or solvent tubing can be used it is necessary to prime the system lsopropanol is recommended as
57. d in the channels see Regular Priming on page 56 Prevent blocking of solvent inlet filters never use the pump without solvent inlet filter Growth of algae should be avoided see Prevent Blocking of Solvent Filters on page 81 e Check purge valve frit and column frit in regular time intervals A blocked purge valve frit can be identified by black or yellow layers on its surface or by a pressure greater than 10 bar when pumping distilled water at a rate of 5 mL min with an open purge valve When using the pump at low flow rates for example 0 2 mL min check all 1 16 inch fittings for any signs of leaks Always exchange the purge valve frit too when exchanging the seals e When using buffer solutions flush the system with water before switching it off The seal wash option should be used when buffer solutions of 0 1 M or higher will be used for long time periods Check the pump plungers for scratches when changing the plunger seals Scratched plungers will lead to micro leaks and will decrease the lifetime of the seal After changing plunger seals apply the seal wear in procedure see Maintenance of a Pump Head Without Seal Wash Option on page 151 1260 Infinity lsoPump QuatPump User Manual 61 4 62 Using the Pump Hints for Successful Use of the Quaternary Pump Always place the solvent cabinet with the solvent bottles on top of the quaternary pump or at a higher level When using salt solution
58. d run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface 1260 Infinity IsoPump QuatPump User Manual 101 7 Error Information General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required 102 1260 Infinity IsoPump QuatPump User Manual Shutdown Error ID 0063 Error Information 7 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a r
59. defect pump main board Degasser limit not reached Error ID 2244 Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative This error is thrown if the degasser does not become ready after 8 min i e is higher than 180 mbar Probable cause 1 Liquid in degasser tubing 2 Leak in degasser tubing or chamber 3 Degasser vacuum pump defect 1260 Infinity lsoPump QuatPump User Manual Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative 123 7 Error Information Module Error Messages 124 1260 Infinity IsoPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual 8 Test Functions and Calibration Introduction 126 System Pressure Test 127 Running the Test 129 Evaluating the Results 130 Potential Causes of System Pressure Test Failure 131 Leak Rate Test 132 Running the Test 134 Evaluating the Results 134 Potential Causes of Leak Rate Test Failure 135 This chapter describes the tests for the module ee Agilent Technologies 125 8 Test Functions and Calibration Introduction Following tests are available in Lab Advisor System Pressure Test Leak Rate Test Table 14 Availability of tests in LabAdvisor version G1310B G1311B Sy
60. delivers flow at a rate of 200 uL min until a pressure of 50 bar below the defined maximum pressure is reached In the second phase the pump delivers a small flow which is increased stepwise If there is a leak in the system the pressure will drop initially as the low flow cannot compensate the leak flow As soon as the pump flow rate exceeds the leak flow rate the pressure will increase again and the test is stopped at about 20 bar below the maximum pressure The point in phase 2 where the lowest pressure is reached and stays constant for a short time corresponds to the leak rate that is provided as a test result A leak rate smaller than 3 uL min is good enough for operating the pump reliably 128 1260 Infinity IsoPump QuatPump User Manual Test Functions and Calibration 8 Running the Test Parts required CAUTION p n Description 01080 83202 Blank nut Damage to pressure sensitive parts Even columns that are suitable for high pressures are sensitive to pressure drops that occur during this test gt Do not include any pressure sensitive parts to the flow path and choose a maximum pressure that is compatible to your system For example do not include columns a standard pressure flow cell up to 20 bar or a 400 bar autosampler to a 600 bar pressure test Running the test from the Agilent Lab Advisor 1 Select the System Pressure Test from the Test Selection menu 2 Start the test and follow the instructions Make s
61. dule 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable Agilent module interface board to general purposes RS 232 cables p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m 1260 Infinity lsoPump QuatPump User Manual 189 11 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog an Center Center Analog 190 1260 Infinity IsoPump QuatPump User Manual Agilent Module to General Purpose Identifying Cables 11 p n 01046 60105 Pin Pin Agilen
62. e 25 26 sound emission special interfaces 218 special settings boot resident 222 W forced cold start 222 wait timeout 122 specification waste physical 25 26 electrical and electronic status indicator 95 equipment 229 stroke length 121 wear in stroke volume 14 17 procedure 160 system pressure test evaluating WEEE directive 229 results 130 weight 25 26 system pressure test 93 127 wrong pump configuration 112 running the test 129 system setup and installation optimizing stack configuration 37 system tool kit 181 T temperature limit exceeded 117 temperature out of range 116 temperature sensor 106 test functions 92 timeout 102 troubleshooting error messages 92 101 status indicators 92 94 1260 Infinity lsoPump QuatPump User Manual Index 237 www agilent com In This Book This manual contains technical information about the Agilent 1260 Infinity Isocratic Pump G1310B and Quaternary Pump G1311B The manual describes the following introduction e site requirements and specifications e installing the pump using the pump e optimizing performance e troubleshooting and diagnostics maintenance e parts and materials for maintenance e identifiying cables appendix Agilent Technologies 2010 2012 2013 Printed in Germany 11 2013 G1310 90016 Rev B EE Agilent Technologies
63. e can also be connected to the system through a LAN cable which requires the installation of a LAN board For more information about connecting the Instant Pilot or Agilent control software refer to the respective user manual Interfaces on page 212 provides information on how to connect external hardware 50 1260 Infinity IsoPump QuatPump User Manual Installing the Pump 3 Flow Connections of the Pump Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch Parts required Description Other modules Parts from starter kits Preparations Pump is installed in the LC system WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere 1260 Infinity IsoPump QuatPump User Manual 51 3 52 Installing the Pump 1 Remove the front cover by pressing the snap fasteners on both sides Figure 12 Removing the Front Corver Place the solvent cabinet on top of the UHPLC stack Put the bottle hea
64. e modules of the Agilent 1260 Infinity LC System in the following configuration see Figure 7 on page 39 and Figure 8 on page 40 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required 38 1260 Infinity IsoPump QuatPump User Manual Installing the Pump 3 Optimizing the Stack Configuration Solvent cabinet Qo Pump Q Si S 900 000 888 Instant Pilot oc p Autosampler Thermostatted column compartment Detector Figure 7 Recommended Stack Configuration for 1260 Infinity Front View 1260 Infinity IsoPump QuatPump User Manual 39 3 Installing the Pump Optimizing the Stack Configuration AC power CAN bus cable to Instant Pilot Remote cable EEEEELLJ CAN bus cable LAN to control software location depends on detector Analog detector signal 1 or 2 outputs per detector Figure 8 Recommended Stack Configuration for 1260 Infinity Rear View 40 1260 Infinity IsoPump QuatPump User Manual Installing the Pump 3 Installation Information on Leak and Waste Handling The Agilent 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed WARNING Toxic flammable and hazardous solvents samples and reagents The handling of
65. e of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 232 1260 Infinity IsoPump QuatPump User Manual Appendix 13 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com 1260 Infinity IsoPump QuatPump User Manual 233 Index Index 8 bit configuration switch without On Board LAN 219 A active inlet valve cartridge 166 active inlet valve 166 178 active seal wash 10 86 adapter 166 Agilent Lab Advisor software 97 Agilent Lab Advisor 97 Agilent oninternet 233 AIV fuse 113 algae growth 81 algae 61 alternative seal material 87 ambient non operating temperature 25 26 ambient operating temperature 25 26 analog output 27 30 analog signal 216 analog cable 190 apg remote 217 AUTO mode 17 AUX output 49 B ball screw drive 14 battery safety information 230 BCD board 234 external contacts 206 BCD cable 195 bench space 24 blockage 115 board HP JetDirect card 208 boards LAN card 208 bottle head assembly 183 buffer application 61 62 buffer solution 10 161 c cable analog 190 BCD 195 CAN 197 external contact 198 interface 47 LAN 197 remote 192 RS 232 199 cables analog 188 BCD 188 CAN 189 external
66. e pulsation 17 27 29 88 pressure range 87 pressure sensor readings 49 pressure signal missing 111 pressure operating range 27 29 priming with a pump 56 proportioning valve high speed 12 PTFE frit 147 pump error messages 110 pump head assembly nosealwash 172 sealwash 174 pump head missing 119 pump piston 61 62 pump seals 61 p urge valve frit 62 purge valve 61 147 radio interference 231 reassembling the pump head 158 recommended pH range 29 remote cable 192 removing pump head assembly 149 repairs replacing firmware 170 170 RS 232C cable 199 communication settings 220 S safety class 227 safety information lithium batteries 230 safety general information 227 standards 25 26 symbols 226 sapphire piston 14 seal wash cartridge exchanging 168 sealwash 10 12 86 whentouse 86 seal alternative material 87 seal wear in 160 seals 61 servo restart failed 118 setable flow range 27 29 shutdown 103 simple repairs 138 site requirements power cords 23 1260 Infinity lsoPump QuatPump User Manual snap fasteners 52 U solvent cabinet 61 62 182 solvent filter checking 142 replacing 142 v unpacking the pump 32 user interfaces 96 solvent filters prevent blocking 81 solvent inlet filters 61 62 valve failed 114 valve frit 147 variable reluctance motor 14 vacuum degasser 10 28 30 61 solvent zero counter 110 solvents change 57 232 variable stroke volume 17 voltage rang
67. e results In gradient mode smaller stroke volumes result in a lower flow ripple improve composition ripple The module uses a processor controlled spindle system for driving its pistons The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume By default the stroke volume for the pump is set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended 1260 Infinity lsoPump QuatPump User Manual 17 1 Introduction System Overview System Overview Leak and Waste Handling The 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed Figure 6 Leak and waste handling concept overview typical stack configuration as an example 18 1260 Infinity IsoPump QuatPump User Manual Introduction 1 The solvent cabinet 1 is designed to store a maximum volume of 6 L solvent The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 5 L For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets a printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet The leak pa
68. e the module on the bench in a horizontal position 2 Ensure the power switch on the front of the module is OFF switch stands out Status lamp Name plate Power switch Serial number Figure 11 Front of Pump 3 Connect the power cable to the power connector at the rear of the module 46 1260 Infinity IsoPump QuatPump User Manual Installing the Pump 3 Installing the Pump 4 Connect the required interface cables to the quaternary pump see Connecting Modules and Control Software on page 48 Configuration switch Slot for interface board oo R CAN Can Analog pressure APG remote RS 232C 3D Asie Techrologies Product No 13418 Seiho OB Paxxt1 Power 5 Connect all capillaries solvent tubes and waste tubing see Flow Connections of the Pump on page 51 6 Press the power switch to turn on the module NOTE The power switch stays pressed in and a green indicator lamp in the power switch is on when the module is turned on When the line power switch stands out and the green light is off the module is turned off 7 Purge the quaternary pump see Inital Priming on page 54 NOTE The pump was shipped with default configuration settings To change these settings see Setting the 8 bit Configuration Switch without On board LAN on page 219 1260 Infin
69. eakages Damage of the pump drive Starting the pump when the pump head is removed may damage the pump drive gt Never start the pump when the pump head is removed 1 Remove the front cover by pressing the clip fasteners on 2 If an active inlet valve is installed disconnect the active both sides of the cover inlet valve cable 1260 Infinity lsoPump QuatPump User Manual 149 9 Maintenance 3 Using a 1 4 inch wrench remove the outlet capillary gt Si v Outlet valve capillary Disconnect the capillary from the outlet valve Remove the waste tubing and disconnect the solvent tubing from the inlet valve If applicable remove tubings from the seal wash support rings Remove the capillary at the bottom of the pump head 8 Using a 4 mm hexagonal key stepwise loosen the two pump head screws and remove the pump head from the pump drive Pump head screws 150 1260 Infinity IsoPump QuatPump User Manual Maintenance 9 Maintenance of a Pump Head Without Seal Wash Option When In case of maintenance or pump head internal leaks Tools required Description Wrench 1 4 inch Hexagonal key 4 mm Parts required p n Description 1 01018 23702 Insert tool 1 5063 6589 Piston seal PTFE carbon filled black pack of 2 default OR 1 0905 1420 PE seals pack of 2 1 5063 6586 Sapphire piston For a complete list of parts see Pump Head Assembly Without Seal Was
70. echanical stop is out of limits pump During initialization the module monitors the drive current If the piston reaches the upper mechanical stop position before expected the motor current increases as the module attempts to drive the piston beyond the mechanical stop This current increase causes the error message to be generated Probable cause Suggested actions 1 Defective pump drive assembly Please contact your Agilent service representative Initialization Failed Error ID 2207 2217 The module failed to initialize successfully within the maximum time window A maximum time is assigned for the complete pump initialization cycle If the time is exceeded before initialization is complete the error message is generated Probable cause Suggested actions 1 Blocked passive inlet valve Exchange the passive inlet valve 2 Defective pump drive assembly Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 1260 Infinity lsoPump QuatPump User Manual 121 7 Error Information Wait Timeout Error ID 2053 When running certain tests in the diagnostics mode or other special applications the pump must wait for the pistons to reach a specific position or must wait for a certain pressure or flow to be reached Each action or state must be completed within the timeout period otherwise the error message is generated Possible Reasons for a Wait
71. echnologies on Internet 233 8 1260 Infinity IsoPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual e Introduction Introduction to the Pump 10 Overview of the Hydraulic Path Hydraulic Path 13 How Does the Pump Work 12 14 How Does Compressibility Compensation Work How Does Variable Stroke Volume Work 17 System Overview 18 Leak and Waste Handling 17 18 This chapter gives an introduction to the module instrument overview and internal connectors ee Agilent Technologies 1 introduction Introduction to the Pump Introduction to the Quaternary Pump The quaternary pump comprises an optional solvent cabinet a vacuum degasser and a four channel gradient pump The four channel gradient pump comprises a high speed proportioning valve and a pump assembly It provides gradient generation by low pressure mixing A solvent cabinet provides enough space for four one liter bottles An active seal wash optional is available when the quaternary pump is used with concentrated buffer solutions Outlet valve Pump head Purge valve Passive inlet valve MCGV Degasser Figure 1 Overview of the Quaternary Pump 10 1260 Infinity IsoPump QuatPump User Manual Introduction 1 Introduction to the Isocratic Pump The isocratic pump has the same operating principle as the quaternary pump but has only one solvent channel that means the composition cannot be changed during a method because there i
72. ed significantly DO NOT apply the seal wear in procedure with PE seals 1260 Infinity lsoPump QuatPump User Manual 87 5 Optimizing Performance Optimize the Compressibility Compensation Setting 88 The compressibility compensation default setting is 100 x 10 bar for the pump This setting represents an average value Under normal conditions the default setting typically reduces the pressure pulsation to values below 1 of system pressure that are sufficient for most applications and for all gradient analyses The compressibility settings can be optimized by using the values for the various solvents described in Table 12 on page 89 If the solvent in use is not listed in the compressibility tables when using isocratic mixtures of solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the compressibility settings When using mixtures of solvents it is not possible to calculate the compressibility of the mixture by interpolating the compressibility values of the pure solvents used in that mixture or by applying any other calculation In these cases the following empirical procedure has to be applied to optimize your compressibility setting Inappropriate settings would mainly affect retention times of peaks eluted at the beginning of a gradient Therefore optimize settings for the solvent at the beginning of the gradient For mixtures containing up to 50 water use
73. emote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing 1260 Infinity lsoPump QuatPump User Manual Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition Check the vacuum degasser for an error condition Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built in 103 7 Error Information Remote Timeout Error ID 0070 A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause Suggested actions 1 Not ready condition in one of the Ensure the instrument showing the not ready instruments connected to the remote line condition is installed correctly and is set up correctly for analysis 2 Defective remote cable Exchange the remote cable 3 Defective components in the instrument Check the instrument for defects refer to the showing the not ready condition instrument s documentation 104 1260 Infinity lsoPump QuatPump User Manual Error Information
74. emove any deposits or layers Cleaning can be done with alcohol or tooth paste Replace piston if scratched Piston surface 3 Using the steel side of the insert tool carefully remove the seal from the pump housing Remove wear retainers if still present Insert tool Piston seal 4 Using the plastic side of the insert tool insert new seals into the pump head Piston seals 1260 Infinity lsoPump QuatPump User Manual 155 9 Maintenance ofaP Maintenance o imp Head with Se 5 Using the steel side of the insert tool remove the seal wash gasket and the wash seal from the support ring The removed seal will be damaged and cannot be re used Seal wash support ring Wash seal 6 Using the plastic side of the insert tool press the new wash seal spring pointing upwards into the recess of the support ring Insert too Wash seal Support ring 7 Place a seal wash gasket in the recess of the support ring Put the seal holder on top of the gasket Seal holder Seal wash gasket gasket Support ring 8 Place the support rings on the piston housing pistons not installed and snap the pump head and piston housing together Note the correct position of the pin on the support ring E Pump head Support ring A Piston housing 156 1260 Infinity IlsoPump QuatPump User Manual Maintenance Maintenance of a Pump Head with Seal Wash Option P
75. ent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 1260 Infinity lsoPump QuatPump User Manual 205 12 Hardware Information Optional Interface Boards BCD External Contact Board The Agilent 1200 Infinity Series modules have one optional board slot that allows to add an interface board to the modules Some modules do not have this interface slot Refer to Interfaces on page 212 for details Optional Interface Boards p n Description G1351 68701 Interface board BCD with external contacts and BCD outputs 2110 0004 Fuse for BCD board 250 mA The BCD board provides a BCD output for the bottle number of the Agilent 1200 Series autosampler and four external contacts The external contact closure contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused Board identification 12 Processor BCD BCD interface register connector Line driver RFI filter 250mA External 4x External contacts lt contact connector DRF filter 206 1260 Infinity IsoPump QuatPump User Manual Hardware Information 12 There are general purpose cables available to connect the BCD output see BCD Cables on page 195 and the external outputs see External Contact Cable on page 198 to external devices Table 16 Detailed connector layout 1200 Pin Signal name BCD digit 1 BCD 5 20 2 BCD 7 80 3 BCD 6 40 4 BCD 4 10 5 BCD 0 1 6 BCD 3 8 7
76. eparate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could r
77. esistance mechanical and thermal stability PEEK is therefore the material of choice for UHPLC and biochemical instrumentation It is stable in a wide pH range and inert to many common solvents There is still a number of known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc 1260 Infinity lsoPump QuatPump User Manual Using the Pump 4 Polyimide Agilent uses semi crystalline polyimide for rotor seals in valves and needle seats in autosamplers One supplier of polyimide is DuPont which brands polyimide as Vespel which is also used by Agilent Polyimide is stable in a pH range between 1 and 10 and in most organic solvents It is incompatible with concentrated mineral acids e g sulphuric acid glacial acetic acid DMSO and THF It is also degraded by nucleophilic substances like ammonia e g ammonium salts in basic conditions or acetates Polyethylene PE Agilent uses UHMW ultra high molecular weight PE PTFE blends for yellow piston and wash seals which are used in 1290 Infinity pumps and for normal phase applications in 1260 Infinity pumps Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12 5 It is compatible to many organ
78. essure of that curve segment corresponds to the flow and leak rate at that time and the leak rate is measured Compare to the description of the system pressure test System Pressure Test on page 127 Subsequently piston 2 is moved to its rear position then piston 2 delivers and the measurement is done as described for piston 1 For a binary pump G1312B C K1312B the test is run for both pump heads for channels A and B 1260 Infinity IsoPump QuatPump User Manual 133 8 Test Functions and Calibration Leak Rate Test Running the Test Parts required p n 01080 83202 Description Blank nut Running the test from the Agilent Lab Advisor 1 Select the Leak Rate Test from the Test Selection menu 2 Start the test and follow the instructions Make sure to release the pressure by slowly opening the purge valve when the test has finished Evaluating the Results Damper Pump chamber 1 Outlet valve Inlet valve Piston 1 134 Results of the leak rate test are the leak rates measured for pistons 1 and 2 as described for the test principle If any of the leak rates exceeds 3 uL min the test will fail 7 s 1M Wa gt To column To waste Pump chamber 2 Purge valve Seal Piston 2 1260 Infinity lsoPump QuatPump User Manual Test Functions and Calibration 8 Potential Causes of Leak Rate Test Failure Secondary Leak If a leak is found for mo
79. esult in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1260 Infinity lsoPump QuatPump User Manual In This Guide In This Guide This manual covers e the Agilent 1260 Infinity Isocratic Pump G1310B and e the Agilent 1260 Infinity Quaternary Pump G1311B 1 Introduction This chapter gives an introduction to the module instrument overview and internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Pump This chapter gives information about the preferred stack setup for your system and the installation of your module 4 Using the Pump This chapter provides information for optimized usage of the module 5 Optimizing Performance This chapter gives hints on how to optimize the performance or use additional devices 6 Troubleshooting and Diagnostics This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces 1260 Infinity Iso
80. finity IsoPump QuatPump User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment 1260 Infinity lsoPump QuatPump User Manual 23 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 1
81. for Maintenance Pump Head Assembly Without Seal Wash 172 Pump Head Assembly with Seal Wash Option 174 Outlet Valve 176 Purge Valve Assembly 177 Active Inlet Valve Assembly 178 HPLC Starter Kit G4201 68707 179 HPLC Starter Kit G4202 68707 180 HPLC System Tool Kit 181 Solvent Cabinet 182 Bottle Head Assembly 183 Hydraulic Path of the Quaternary Pump 184 Hydraulic Path of the Isocratic Pump 186 This chapter provides information on parts for maintenance Ee Agilent Technologies 171 10 Parts for Maintenance Pump Head Assembly Without Seal Wash Item p n G1312 60056 1 5063 6586 2 G1311 60002 3 5067 1560 4 5062 2484 5 5042 8952 6 5063 6589 OR 0905 1420 7 G1311 25200 8 G1312 60066 G1312 60025 G1312 60020 9 G1312 60067 10 5042 1303 11 G1312 60061 12 0515 2118 Description Pump Head 1200 SL without Seal Wash Sapphire piston Piston housing Support Ring SL no seal wash Gasket seal wash pack of 6 Seal holder Piston seal PTFE carbon filled black pack of 2 default PE seals pack of 2 Pump chamber housing Passive inlet valve 1220 1260 Active inlet valve body without cartridge OPTIONAL Cartridge for active inlet valve 600 bar OPTIONAL Outlet valve 1220 1260 Lock screw Purge valve 1260 Pump head screw M5 60 mm The Pump Head 1200 SL without Seal Wash G1312 60056 includes items 1 7 10 and 12 For piston seals see Choosing the Right Pump Seals on page 87 172
82. g meaning and function Indicates that an External Contacts board is installed ae The level of solvent in the bottle is denoted by the green area when the solvent level falls below the specified volume the tA area turns yellow when the bottle is empty the area turns red Clicking on the solvent bottle displays the Bottle Fillings dialog box The tooltip for the bottle shows the solvent name EN The pressure setpoints The red line shows the current maximum pressure limit the green area shows the current pressure also shown as text The current solvent flow rate in mL min is displayed above the pressure display 1260 Infinity IsoPump QuatPump User Manual Using the Pump 4 Instrument Signals The following pump signals are displayed Table 6 Pump signals isocratic pump Flow The current solvent flow rate in mL min Pressure The current pump ressure in bar psi or MPa see Instrument Configuration on page 65 Ripple The current ripple in Pressure Limit The current maximum pressure limit Table 7 Pump signals quaternary pump Flow The current solvent flow rate in mL min Pressure The current pump ressure in bar psi or MPa see Instrument Configuration on page 65 Tuning Indicates the tuning efforts of 1290 Infinity pumps For pumps operating as expected the signal should stay in a range of 1 to 1 within the full scale of 2 to 2 Pressure Limit The
83. h on page 172 Preparations Switch off pump at the main power switch e Remove the front cover Removing the Pump Head Assembly on page 149 1260 Infinity lsoPump QuatPump User Manual 151 9 Maintenance nro nf a D lance otar imp Head Without Seal Wash Opti 1 Place the pump head on a flat surface Loosen the lock screw two turns and while holding the lower half of the assembly piston housing carefully pull the pump housing away from the piston housing Pump housing Ke No Piston i ousing 4 2 Remove the support rings from the piston housing and lift the housing away from the pistons Piston housing Piston DY 2 3 Check the piston surface and remove any deposits or layers Cleaning can be done with alcohol or tooth paste Replace piston if scratched Piston surface 4 Using the steel side of the insert tool carefully remove the seal from the pump housing Remove wear retainers if still present Insert tool 152 1260 Infinity IsoPump QuatPump User Manual Maintenance 9 5 Using the plastic side of the insert tool insert new seals into the pump head Piston seals 7 Sl 6 Reassemble the pump head assembly Note the correct position of the pin on the support ring Pump head Support ring i Piston housing 7 Insert the pistons and carefully press them into the seals Pistons 8 Tighten the lock screw SKS i gt lt 4
84. h 100 Solvent A always delivers the remaining volume 100 B C D The check boxes allows you to turn the solvent channels on checked or off cleared The text boxes allow you to type a brief description of each of the solvents Stoptime 0 01 99999 minor The stoptime sets a time limit for your analysis After the stoptime all AsInjector NoLimit gradients are stopped and the pump parameters return to their initial an infinite run time values The pump can be used as a stoptime master for the complete analytical system The pump also stops the detectors if they have a No Limit stoptime setting If no limit is given a method will have to be stopped manually Posttime 0 01 99999 minor Your instrument remains in a not ready state during the posttime to delay Off 0 0 min the start of the next analysis You can use the Posttime to allow your column to equilibrate after changes in solvent composition for example after gradient elution 70 1260 Infinity lsoPump QuatPump User Manual Table9 Method parameters Using the Pump 4 Parameter Limits Description Pressure Limits Max 600 bar Sets the maximum and minimum pressure limits for the pump 8700 psi for flow Max is the maximum pressure limit at which the pump will switch rates up to itself off protecting the analytical system against over pressure 5 mL min 200 bar Minis the minimum limit at which the pump will switch itself off for 2900 psi for flow exam
85. he Hydraulic Path Hydraulic Path Damper To sampling unit and column Outlet valve From solvent bottles To waste Figure 3 Hydraulic Path of the Isocratic Pump 1260 Infinity lsoPump QuatPump User Manual 13 1 14 Introduction Vacuum chamber Degasser From solvent bottles To sampling unit and column To waste Figure 4 Hydraulic Path of the Quaternary Pump How Does the Pump Work In the quaternary pump the liquid runs from the solvent reservoir through the degasser to the MCGV and from there to the inlet valve For the isocratic pump the solvent bottle is directly connected to the inlet valve The pump assembly comprises two substantially identical piston chamber units Both piston chamber units comprise a ball screw drive and a pump head with one sapphire piston for reciprocating movement in it A servo controlled variable reluctance motor drives the two ball screw drives in opposite directions The gears for the ball screw drives have different circumferences ratio 2 1 allowing the first piston to move at twice the speed of the second piston The solvent enters the pump head 1260 Infinity lsoPump QuatPump User Manual Introduction 1 Overview of the Hydraulic Path close to the bottom limit and leaves it at its top The outer diameter of the piston is s
86. head Using the 14 mm wrench turn the nut until it is hand tight 10 Position the valve such that the solvent inlet tube connection points towards the front 11 Using the 14 mm wrench tighten the nut by turning the valve into its final position not more than a quarter turn 12 Reconnect the adapter at the active inlet valve 13 Reconnect the solvent inlet tube to the adapter Reconnect the active inlet valve cable to the connector in the Z panel 14 Reinstall the front cover 15 Purge the system with 30 mL of solvent in order to achieve a low pressure ripple see Regular Priming on page 56 1260 Infinity lsoPump QuatPump User Manual 167 9 Maintenance Exchanging the Seal Wash Cartridge Parts required p n Description 5067 4793 Peristaltic Pump with Fixation Springs Preparations Switch off pump at the main power switch Remove the front cover 1 Remove the wash solvent tubings from the support ring 2 Unclip the peristaltic pump cartridge from the module housing and remove it inlet and outlet 168 1260 Infinity IsoPump QuatPump User Manual Maintenance 9 3 Put the new peristaltic pump cartridge onto the rod of the pump motor and push the plastic clips into the module housing 4 Connect the peristaltic pump tubes to the middle nozzles of the support rings Connect the left nozzle to the wash solvent reservoir and the right one to the waste container To wash solvent reser
87. ic solvents used in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated acids and bases For normal phase applications the maximum pressure should be limited to 200 bar Tantalum Ta Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide It can be corroded by strong bases e g hydroxide solutions gt 10 diethylamine It is not recommended for the use with fluoric acid and fluorides Stainless Steel ST Stainless steel is inert against many common solvents It is stable in the presence of acids and bases in a pH range of 1 to 12 5 It can be corroded by acids below pH 2 3 It can also corrode in following solvents Solutions of alkali halides their respective acids for example lithium iodide potassium chloride and so on and aqueous solutions of halogens 1260 Infinity lsoPump QuatPump User Manual 77 4 78 Using the Pump High concentrations of inorganic acids like nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCly 02 gt 2 COCl 2 HCl This re
88. iew This checkbox allows you displaying parameter changes instead of a time table 1260 Infinity IsoPump QuatPump User Manual 73 4 Using the Pump Solvent Information 74 Observe the following recommendations on the use of solvents Follow recommendations for avoiding the growth of algae see Algae Growth in HPLC Systems on page 80 Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections 1260 Infinity IsoPump QuatPump User Manual Using the Pump 4 Materials in Flow Path Following materials are used in the flow path of this module Table 11 Materials in flow path Part Materials Degasser chamber TFE PDD Copolymer PFA internal tubings PEEK inlets FEP tubings ETFE fittings MCGV SST PTFE Passive inlet valve SST gold sapphire ruby ceramic PTFE Outlet valve SST gold ruby ZrOj based ceramic tantalum Adapter SST gold Pump head body SST Pistons Sapphire Piston seals wash seals PTFE SST reversed phase or UHMW PE SST normal phase Pressure sensor SST Purge valve SST gold PTFE ceramic Damping unit SST gold Capillaries fittings SST Tubings PTFE 1260 Infinity IsoPump QuatPump
89. ilent LabAdvisor leak detection safe leak handling leak output signal for shutdown of the pumping system Low voltage in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre defined and user settable limits and feedback messages Electronic records of maintenance and errors All materials are recyclable For use with flow rates below 500 pL min a vacuum degasser is required 1260 Infinity IsoPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual 3 Installing the Pump Unpacking the Pump 32 Delivery Checklist 33 Accessory Kit 36 Optimizing the Stack Configuration 37 One Stack Configuration 38 Installation Information on Leak and Waste Handling 41 Installing the Pump 45 Connecting Modules and Control Software 48 Connecting Modules 48 Connecting a Vacuum Degasser 49 Connecting Control Software and or G4208A Instant Pilot 50 Flow Connections of the Pump 51 Priming the System 54 Inital Priming 54 Regular Priming 56 Changing Solvents 57 This chapter gives information about the preferred stack setup for your system and the installation of your module oh Agilent Technologies 31 3 Installing the Pump Unpacking the Pump If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the i
90. in a prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is in a not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e a red LED may indicate a problem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold start see Special Settings on page 222 Then try a firmware update see Replacing the Module Firmware on page 170 If this does not help a main board replacement is required 1260 Infinity lsoPump QuatPump User Manual 95 6 Troubleshooting and
91. inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications 1260 Infinity lsoPump QuatPump User Manual 79 4 Using the Pump Algae Growth in HPLC Systems 80 The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing Blocked solvent filters or deposits on inlet or outlet valves resulting in unstable flow composition or gradient problems or a complete failure of the pump Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure PTFE frits blockage leading to increased system pressure Column filters to plug giving high system pressure Flow cell windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the flow path this problem is less common How to Prevent and or Reduce the Algae Problem Always use freshly prepared solvents especially use demineralized water which was filtered
92. ing range up to 60 MPa 600 bar 8700 psi up to 5 mL min Operating range up to 20 MPa 200 bar 2950 psi up to 10 mL min lt 2 amplitude typically lt 1 3 or lt 0 3 MPa 3 bar 44 psi whatever is greater at 1 mL min isopropanol at all pressures gt 1 MPa 10 bar 145 psi User selectable based on mobile phase compressibility 1 0 12 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel Low pressure quaternary mixing gradient capability using proprietary high speed proportioning valve 600 900 uL dependent on back pressure measured with water at 1 mL min water caffeine tracer 0 95 or 5 100 user selectable lt 0 2 RSD or lt 0 04 min SD whatever is greater at 1 mL min based on retention time at constant room temperature Number of channels 4 Internal volume per channel 1 5 mL Agilent control software e g ChemStation EZChrom OL MassHunter 1260 Infinity lsoPump QuatPump User Manual 29 2 30 Site Requirements and Specifications Table4 Performance Specifications Agilent 1260 Infinity Quaternary Pump G1311B Local control Analog output Communications Safety and maintenance GLP features Housing Agilent Instant Pilot For pressure monitoring 2 mV bar one output Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN optional Extensive diagnostics error detection and display through Ag
93. ions Table3 Performance Specification Agilent 1260 Infinity lsocratic Pump G1310B GLP features Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre defined and user settable limits and feedback messages Electronic records of maintenance and errors Housing All materials are recyclable For use with flow rates below 500 pL min a vacuum degasser is required 28 1260 Infinity IsoPump QuatPump User Manual Site Requirements and Specifications 2 Performance Specifications Agilent 1260 Infinity Quaternary Pump G1311B Table 4 Performance Specifications Agilent 1260 Infinity Quaternary Pump G1311B Type Hydraulic system Setable flow range Flow range Flow precision Flow accuracy Pressure operating range Pressure pulsation Compressibility compensation Recommended pH range Gradient formation Delay volume Composition range Composition precision Integrated degassing unit Control Specification Dual piston in series pump with servo controlled variable stroke drive power transmission by gears and ball screws floating pistons Set points 0 001 10 mL min in 0 001 mL min increments 0 2 10 0 mL min lt 0 07 RSD or 0 02 min SD whatever is greater based on retention time at constant room temperature 1 or 10 pL min whatever is greater pumping degassed H 0 at 10 MPa 100 bar Operat
94. ions of 0 1 M or higher will be used for long time periods Check the pump pistons for scratches when changing the piston seals Scratched pistons will cause micro leaks and will decrease the lifetime of the seal Pressurize the system according to the wear in procedure after changing the piston seals see Maintenance of a Pump Head Without Seal Wash Option on page 151 Consider recommendations given in the solvent information section see Solvent Information on page 74 1260 Infinity IsoPump QuatPump User Manual Usingthe Pump 4 Setting up the Pump with the G4208A Instant Pilot Generic operation of the G4208A Instant Pilot is covered in the Agilent Instant Pilot G4208A User s Guide G4208 90006 Details about setting up module specific parameters can be found in the Instant Pilot online help The pump parameters are described in detail in Overview on page 64 1260 Infinity IsoPump QuatPump User Manual 63 4 Using the Pump Setting up the Pump with the Instrument Control Interface Overview Parameters described in following sections is offered by the instrument control interface and can usually be accessed through Agilent instrument control software For details please refer to manuals and online help of respective user interfaces 64 1260 Infinity IsoPump QuatPump User Manual Using the Pump 4 Instrument Configuration Use the Instrument Configuration dialog box to examine and if necessary modify
95. ire a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as 1260 Infinity lsoPump QuatPump User Manual 215 12 Hardware Information 216 Table 19 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 n 2 RX TX 3 X 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 M 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 28 RS 232 Cable Analog Signal Output The ana
96. istons EN ES Dip Aa 6 JA R waa zA f 4 7 AL gt W 9 Insert the pistons and carefully press them into the seals 10 Tighten the lock screw Lock screw a OO 1260 Infinity lsoPump QuatPump User Manual 157 9 Maintenance Reinstalling the Pump Head Assembly When When reassembling the pump Tools required p n Description 8710 2392 Hex key 4 mm15 cm long T handle Parts required p n Description 1 79846 65501 Pump head grease 1 If needed apply a small amount of grease on the back of 2 Slide the pump head assembly onto the pump drive and the screws Normally the grease added during manufacturing is sufficient for a long time use a 4 mm hexagonal key to tighten the pump head screws stepwise with increasing torque max 5 Nm Metering drive Pump head Pump head screws 158 1260 Infinity IsoPump QuatPump User Manual Maintenance 9 Reinstalling the Pump Head Assembly 3 Reconnect all capillaries tubes and if installed the 4 Reinstall the front cover active inlet valve cable to its connector 1260 Infinity lsoPump QuatPump User Manual 159 9 Maintenance Seal Wear in Procedure Parts required CAUTION p n Description 0100 1847 Adapter AIV to solvent inlet tubes 5022 2159 Restriction capillary Seal damage This procedure is required for black PTFE seals standard applications p n 5063 6589
97. itch stands out 4 Plug an APG cable into the APG remote connector at the rear of the degasser 5 Connect the APG cable to the APG remote connector of the pump see Figure 8 on page 40 6 Press in the power switch to turn on the vacuum degasser The AUX output is intended for troubleshooting It provides a DC voltage in the range of 0 1 V which is proportional to the vacuum level in the degasser chambers 1260 Infinity lsoPump QuatPump User Manual 49 3 Installing the Pump Connecting Control Software and or G4208A Instant Pilot With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN Usually the detector is producing the most data in the stack followed by the pump and it is therefore highly recommended to use either of these modules for the LAN connection 1 Ensure the power switches on the front of the modules in the stack are OFF switches stand out 2 If there are no other 1260 with LAN port in the HPLC stack install a G1369B LAN board into the extension slot of the pump Connect the LAN enabled module with a LAN cable to the data system 4 Plug the CAN connector of the Instant Pilot into any available CAN port of the 1260 system 5 Plug a CAN cable into the CAN connector of the Instant Pilot 6 Connect the CAN cable to the CAN connector of one of the modules 7 Press in the power switches to turn on the modules The Agilent control softwar
98. ity lsoPump QuatPump User Manual 47 3 Installing the Pump Connecting Modules and Control Software WARNING Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Connecting Modules 1 Place the individual modules in a stack configuration as shown in Figure 7 on page 39 2 Ensure the power switches on the front of the modules are OFF switches stand out 3 Plug a CAN cable into the CAN connector at the rear of the respective module except vacuum degasser 4 Connect the CAN cable to the CAN connector of the next module see Figure 8 on page 40 5 Press in the power switches to turn on the modules 48 1260 Infinity lsoPump QuatPump User Manual Installing the Pump 3 Connecting a Vacuum Degasser The quaternary pump has a built in degasser For the isocratic pump an external degasser can be used and a pump upgrade to the quaterary pump including a built in degasser is possible 1 Place the vacuum degasser in the stack of modules as shown in Figure 7 on page 39 2 Connect the bottle head assembly in the solvent reservoir to the degasser inlet Connect the degasser outlet to the inlet valve of the pump 3 Ensure the power switch at the front of the vacuum degasser is OFF sw
99. le 197 communication interface board 208 eak rate test evaluating results 134 running the test 134 eak sensor open 107 eak sensor short 107 eak 106 ine frequency 25 26 ine voltage 25 26 ithium batteries 230 ost CAN partner 105 ow pressure pump main board LPM 202 235 Index M main power supply 202 maintenance feedback 223 overview 140 replacing firmware 170 170 MCGV fuse 113 MCGV 12 message ignition without cover 109 109 remote timeout 104 missing parts 33 missing pressure reading 112 motor drive power 115 multi channel gradient valve MCGV 161 non operating altitude 25 26 non operating temperature 25 26 0 operating Altitude 25 26 operating temperature 25 26 operational hints MCGV 85 operational pressure range 29 optimization stack configuration 37 optional interface board 164 202 outlet valve 145 176 overview pump 12 P packaging damaged 32 parts identification cables 187 parts 236 active inlet valve 178 bottle head assembly 183 damaged 33 hydraulic path 184 186 missing 33 outlet valve 176 pump head with seal wash 174 pump head without seal wash 172 solvent cabinet 182 system tool kit 181 passive inlet valve 143 pH range 29 physical specifications 25 26 piston chamber 12 piston 14 61 62 power consideration 22 power consumption 25 26 power cords 23 power supply indicator 94 power switch 46 pressure above upper limit 110 pressure below lower limit 111 pressur
100. ler s needle wash 5 Condense drain outlet of the autosampler cooler 6 Waste tube of the purge valve 7 Waste tube 1 Stack the modules according to the adequate stack configuration The leak pan outlet of the upper module must be vertically positioned above the leak tray of the lower module see Figure 9 on page 42 2 Connect data and power cables to the modules see section Installing the Module below 3 Connect capillaries and tubes to the modules see section Flow Connections to the module below or the relevant system manual WARNING Toxic flammable and hazardous solvents samples and reagents gt Keep solvent path free from blockages gt Keep the flow path closed in case the pump in the system is equipped with a passive inlet valve solvent may leak out due to hydrostatic pressure even if your instrument is off gt Avoid loops gt Tubes must not sag gt Do not bend tubes gt Do not immerse tube end in waste liquid gt Do not intubate tubes in other tubes gt For correct tubing follow instructions on label attached to the module 1260 Infinity lsoPump QuatPump User Manual 43 3 Installing the Pum tallation Inforn Warning Attention Warnung Attenzione Advertencia Disconnect power cord before moving D branchez le cordon secteur avant tout d placement Entfernen Sie das Netzkabel bevor Sie das Gerat bewegen Scollegare il cavo di alimentazione prima di spostare lo
101. livering to the system After a controller defined stroke length that depends on the flow rate the drive motor is stopped and the passive inlet valve closes The motor direction is reversed and moves the first piston up until it reaches the stored upper limit and at the same time the second piston moves downwards Then the sequence starts again moving the pistons up and down between the two limits During the up movement of the first piston the solvent in the pump chamber is pressed through the outlet valve into the second pump chamber The second piston draws in half of the volume displaced by the first piston and the remaining half volume is directly delivered to the system During the drawing stroke of the first piston the second piston delivers the drawn volume to the system Quaternary pump For solvent compositions from the solvent bottles A B C D the controller divides the length of the intake stroke in certain fractions in which the gradient valve connects the specified solvent channel to the pump input For specifications of the isocratic pump see Table 3 on page 27 For specifications of the quaternary pump see Table 4 on page 29 1260 Infinity lsoPump QuatPump User Manual Introduction 1 How Does Compressibility Compensation Work The compressibility of the solvents in use will affect retention time stability when the back pressure in the system changes for example ageing of column In order to minimize this effect the
102. ll sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 4 Improperly positioned cables or wires Please contact your Agilent service representative obstructing fan blades Open Cover Error ID 0205 The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during operation Please contact your Agilent service representative 2 Foam not activating the sensor Please contact your Agilent service representative 3 Defective sensor or main board Please contact your Agilent service representative 4 Rear of the module is exposed to strong Ensure that the rear of module is not directly direct sunlight exposed to strong sunlight 1260 Infinity lsoPump QuatPump User Manual
103. log signal output can be distributed to a recording device For details refer to the description of the module s main board 1260 Infinity IsoPump QuatPump User Manual Hardware Information 12 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUTDOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits o
104. losion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 230 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1260 Infinity IsoPump QuatPump User Manual Appendix 13 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 1260 Infinity lsoPump QuatPump User Manual 231 13 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directiv
105. lve indicates blockage Leaks at lower pump head side unstable retention times pressure ripple unstable run Leak Rate Test for verification Seal life time shorter than usual check pistons while changing the seals Error condition indicated by red status indicator 140 1260 Infinity IsoPump QuatPump User Manual Maintenance 9 Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 1260 Infinity lsoPump QuatPump User Manual 141 9 Maintenance Checking and Replacing the Solvent Filter When Parts required CAUTION A functional solvent filter is essential for a good pump performance and for protecting the LC system If solvent filter is blocked p n Description 5041 2168 Solvent inlet filter 20 um pore size See Bottle Head Assembly on page 183 for related parts Small particles can permanently block the capillaries and valves of the module Damage of the module gt Always filter solvents gt Never use the module without solvent inlet filter 142 If the filter is in good condition the solvent will freely drip out of the solvent tube h
106. lve If there are still signs of a leak exchange the appropriate seal purge valve inlet valve outlet valve 4 Defective pump seals Exchange the pump seals 1260 Infinity IsoPump QuatPump User Manual Leak Sensor Open Error ID 0083 Error Information 7 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause 1 Leak sensor not connected to the main board 2 Defective leak sensor 3 Leak sensor incorrectly routed being pinched by a metal component Leak Sensor Short Error ID 0082 Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause 1 Defective leak sensor 2 Leak sensor incorrectly routed being pinched by a metal component 1260 Infinity lsoPump QuatPump User Manual
107. ly Switch off pump at the main power switch and unplug the power cable Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages The active inlet valve can be installed for highest method backward compatibility or special applications By default 1260 Infinity pumps do not have an active inlet valve If an AIV shall be installed please contact your Agilent service representative Remove the front cover 2 Unplug the active inlet valve cable from the connector 166 Disconnect the solvent inlet tube from the inlet valve be aware that solvent may leak out of the tube due to hydrostatic flow Unscrew the adapter from the active inlet valve 1260 Infinity IsoPump QuatPump User Manual Maintenance 9 5 Using a 14 mm wrench loosen the active inlet valve and remove the valve from the pump head Active inlet valve cartridge 7 g O HP a ANE f Active inlet valve f O pa E E Figure 16 Active Inlet Valve Assembly 6 Using a pair of tweezers remove the valve cartridge from the actuator assembly 7 Before inserting the new valve cartridge clean the area in the actuator assembly Flush the cartridge area thoroughly with alcohol 8 Insert a new cartridge into the actuator assembly make sure the valve cartridge is completely inserted into the actuator assembly 9 Insert the new valve into the pump
108. maller than the inner diameter of the pump head chamber allowing the solvent to fill the gap inbetween The first piston has a stroke volume in the range of 20 100 uL depending on the flow rate The microprocessor controls all flow rates in a range of 1 uL min 10 mL min The inlet of the first pumping unit is connected to the passive inlet valve The outlet of the first piston chamber unit is connected through the outlet valve and the damping unit to the inlet of the second piston chamber unit The outlet of the purge valve assembly is then connected to the following chromatographic system Pump chamber 1 Pump chamber 2 Purge valve gt Tocolumn Inlet valve To waste From solvent bottle degasser Seal Piston 1 Piston 2 Ball screw drive Gear Motor with encoder Figure 5 Principle of the Pump 1260 Infinity IsoPump QuatPump User Manual 15 1 16 Introduction When turned on the pump runs through an initialization procedure to determine the upper dead position of the first piston The first piston moves slowly upwards into the mechanical stop of the pump chamber and from there it moves back for a defined distance The controller stores this piston position in memory After this initialization the pump starts operation with the set parameters The passive inlet valve opens and the down moving piston draws solvent into the first pump chamber At the same time the second piston moves upwards de
109. mit Blockages in the flow path are usually detected by the pressure sensor in the damper which result in the pump switching off when the upper pressure limit is exceeded If a blockage occurs before the damper the pressure increase cannot be detected by the pressure sensor and the module will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the module is switched off and the error message is generated Probable cause 1 Flow path blockage in front of the damper 2 Blocked passive inlet valve 3 Blocked outlet valve 4 High friction partial mechanical blockage in the pump drive assembly 5 Defective pump drive assembly 6 Defective main board 1260 Infinity lsoPump QuatPump User Manual Suggested actions Ensure the capillaries and frits between the pump head and damper inlet are free from blockage Exchange the passive inlet valve Exchange the outlet valve Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly Please contact your Agilent service representative Please contact your Agilent service representative 115 7 Error Information Inlet Valve Missing Error ID 2048 2052 The active inlet valve in the module is missing or defective The processor checks the presence of the active inlet valve connector every 2 s If the connector is not detected by the proce
110. mit Probable cause Suggested actions 1 Lower pressure limit set too high Ensure the lower pressure limit is set to a value suitable for the analysis 2 Air bubbles in the mobile phase Make sure that the degasser is in flow path and works correctly Purge the module e Ensure solvent inlet filters are not blocked 3 Leak Inspect the pump head capillaries and fittings for signs of a leak Purge the module Run a pressure test to determine whether the seals or other module components are defective 4 Defective damper Please contact your Agilent service representative 5 Defective main board Please contact your Agilent service representative Pressure Signal Missing Error ID 2016 The pressure signal of the damper is missing The pressure signal of the damper must be within a specific voltage range If the pressure signal is missing the processor detects a voltage of approximately 120 mV across the damper connector Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative 1260 Infinity lsoPump QuatPump User Manual 111 7 Error Information Missing Pressure Reading Error ID 2054 The pressure readings read by the pump ADC analog digital converter are missing The ADC reads the pressure signal of from the damper every lms If the readings are missing for longer than 10 s the erro
111. module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 210 1260 Infinity IsoPump QuatPump User Manual Hardware Information 12 Electrical Connections Rear View of the Module SSE Agilent Tocheologios Product No G1311B MADANM Senai No DE9xxx110 LLN fg lowe Webnategion AIT Me iron Made in Germany Figure 27 Rear view of the pump 1260 Infinity IsoPump QuatPump User Manual 211 12 Hardware Information Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table 18 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN optional on board RS 232 Analog APG Remote Special Pumps G1310B Iso Pump 2 G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump Yes No G4220A B Bin Pump 2 No G4204A Quat Pump Yes G1361A Prep Pump 2 Yes No Samplers G1329B ALS 2 G2260A Prep ALS Yes No G1364B FC PS 2 G1364C FC AS G1364D FC u
112. mp head assembly or pump drive assembly 2 Partial blockage of the flowpath in front of Ensure the outlet valve is not blocked the damper 3 Defective pump drive assembly Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative 1260 Infinity IsoPump QuatPump User Manual 117 7 118 Error Information Servo Restart Failed Error ID 2201 2211 The pump motor in the module was unable to move into the correct position for restarting When the module is switched on the first step is to switch on the C phase of the variable reluctance motor The rotor should move to one of the C positions The C position is required for the servo to be able to take control of the phase sequencing with the commutator If the rotor is unable to move or if the C position cannot be reached the error message is generated Probable cause 1 Disconnected or defective cable 2 Blocked passive inlet valve 3 Mechanical blockage of the module 4 Defective pump drive assembly 5 Defective main board Suggested actions Please contact your Agilent service representative Exchange the passive inlet valve Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly Please contact your Agilent service representative Please contact your Agilent service representative 1260 Infinity lsoPump QuatP
113. mpartment and connections exceeds the test limit After isolating and fixing the cause of the leak repeat the System Pressure Test to confirm the system is pressure tight Probable cause Suggested actions 1 Purge valve open Close the purge valve 2 Loose or leaky fittings Tighten the fitting or exchange the capillary 3 Pump Damaged pump seals or pistons Run the Leak Rate Test to confirm the leak 4 Loose purge valve Tighten the purge valve nut 14 mm wrench 5 Autosampler Loose or leaky fitting Tighten or exchange the fitting or capillary 6 Autosampler Rotor seal injection valve Exchange the rotor seal 7 Autosampler Damaged metering seal or Exchange the metering seal Check the piston piston for scratches Exchange the piston if required 8 Autosampler Needle seat Exchange the needle seat 9 Column compartment Loose or leaky fitting Tighten or exchange the fitting or capillary 10 Column compartment Rotor seal in optional Exchange the rotor seal valve 1260 Infinity lsoPump QuatPump User Manual 131 Test Functions and Calibration Leak Rate Test 132 Introduction The Leak Rate Test is used for verifying the internal tightness of the pump and helps identifying parts which may have caused a leak System requirements Minimum software revisions e Lab Advisor B 01 04 SP1 G1310B Isocratic Pump G1311B Quaternary Pump G4280B Isocratic Pump G4281B Gradient Pump e Lab Advisor B 01 04 SP2 G131
114. n 2 individually designed in each module guides solvents to the front of the module The concept covers also leakages on internal parts e g the detector s flow cell The leak sensor in the leak pan stops the running system as soon as the leak detection level is reached The leak pan s outlet port 8 A guides excessive overfill from one module to the next as the solvent flows into the next module s leak funnel 8 B and the connected corrugated waste tube 3 C The corrugated waste tube guides the solvent to the next lower positioned module s leak tray and sensor The waste tube of the sampler s needle wash port 4 guides solvents to waste The condense drain outlet of the autosampler cooler 5 guides condensate to waste The waste tube of the purge valve 6 guides solvents to waste The waste tube connected to the leak pan outlet on each of the bottom instruments 7 guides the solvent to a suitable waste container 1260 Infinity lsoPump QuatPump User Manual 19 1 20 Introduction System Overview 1260 Infinity IsoPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual 00 e 7 e Site Requirements and Specifications amp i Site Requirements 22 Physical Specifications Isocratic Pump 25 Physical Specifications Quaternary Pump 26 Performance Specifications 27 This chapter provides information on environmental requirements physical and performance specifications
115. n HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia Zirconium Oxide ZrO Zirconium Oxide is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fluorinated polymers PTFE PFA FEP FFKM Fluorinated polymers like PTFE polytetrafluorethylene PFA perfluoroalkoxy and FEP fluorinated ethylene propylene are inert to almost all common acids bases and solvents FFKM is perfluorinated rubber which is also resistant to most chemicals As an elastomer it may swell in some organic solvents like halogenated hydrocarbons TFE PDD copolymer tubings which are used in all Agilent degassers except G1322A are not compatible with fluorinated solvents like Freon Fluorinert or Vertrel They have limited life time in the presence of Hexafluoroisopropanol HFIP To ensure the longest possible life with HFIP it is best to dedicate a particular chamber to this solvent not to switch solvents and not to let dry out the chamber For optimizing the life of the pressure sensor do not leave HFIP in the chamber when the unit is off Sapphire Ruby and Al 03 based ceramics Sapphire ruby and ceramics based on aluminum oxide AlyOg are
116. n and the B channel of the pump is used for the organic solvent If used in this configuration the salt will fall back into the aqueous solution and will be dissolved When using the pump in a different configuration e g D salt solution A organic solvent the salt can fall into the port of the organic solvent and may lead to performance problems When using salt solutions and organic solvents it is recommended to connect the salt solution to one of the bottom ports of the MCGV and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the salt solution channel Regular flushing with water of all MCGV channels is recommended to remove all possible salt deposits in the valve ports Precipitations formed during the mixing of buffers and organic solvents which do not dissolve salts may cause a loss of pump performance flow retention time stability a blockage or internal leak of the pump Avoid the use of such solvent combinations as they can cause irreproducible chromatographic results The use of an Inline filter G1311 60006 can avoid or reduce such effects by filtering crystals and re dissolving them over time 1260 Infinity IsoPump QuatPump User Manual 85 5 Optimizing Performance When to use the Seal Wash Function 86 Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump The seal wash function allows to maintain the
117. nic copies are available on the Internet gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly 60 Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets For details on correct installation see Installation Information on Leak and Waste Handling on page 41 1260 Infinity lsoPump QuatPump User Manual Using the Pump 4 Hints for Successful Use of the Pump Hints for Successful Use of the Isocratic Pump e Always place solvent cabinet with the solvent bottle s on top of the pump or at a higher level e When using salt solutions and organic solvents in the Agilent 1260 Infinity Isocratic Pump it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the salt solution channel Regular flushing with water of all MCGV channels is recommended to remove all possible salt deposits in the valve ports Before operating the pump flush the vacuum degasser with at least two volumes 30 mL especially when turned off for a certain length of time for example during the night and volatile solvent mixtures are use
118. nstrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 32 1260 Infinity IsoPump QuatPump User Manual Installing the Pump 3 Delivery Checklist General Ensure all parts and materials have been delivered with the pump For checking the completeness of your specific shipment please use the list included in your shipment To aid in parts identification please refer to chapter Parts and Materials for Maintenance Please report missing or damaged parts to your local Agilent Technologies sales and service office 1260 Infinity lsoPump QuatPump User Manual 33 3 Installing the Pump G1310B Isocratic Pump Delivery Checklist p n G1310B G4203 68708 959961 902 699975 902 883975 902 G4201 68707 G4202 68707 G1311 60003 G4800 64500 5067 4770 G1369C M8500A Description Agilent 1260 Infinity lsocratic Pump HPLC System Tool Kit OPTIONAL Column Eclipse Plus C18 4 6 x 100 mm 3 5 pm OPTIONAL Column Poroshell 120 EC C18 4 6 x 50 mm 2 7 um OPTIONAL Column SB C18 4 6 x 150 mm 5 pm OPTIONAL HPLC Starter Kit incl 0 17 mm i d cap OP
119. on page 25 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 24 1260 Infinity IsoPump QuatPump User Manual Site Requirements and Specifications 2 Physical Specifications lsocratic Pump Table 1 Physical Specifications Type Specification Comments Weight 11 kg 25 Ibs Dimensions 180 x 345 x 435 mm height x width x depth 7 0 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 180 VA 55 W 188 BTU Maximum Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL 4 55 C 39 131 F 40 70 C 40
120. onents can cause electrostatic discharge ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components 1 Switch off the pump at the main power switch unplug the pump from line power 2 Disconnect cables from the interface board connectors 164 1260 Infinity lsoPump QuatPump User Manual Maintenance 9 Exchanging the Optional Interface Board 3 Loosen the screws Slide out the interface board from the pump BCD interface board Figure 15 Exchanging the Interface Board 4 Install the new interface board Secure screws 5 Reconnect the cables to the board connector 6 Reconnect the pump to line power 1260 Infinity lsoPump QuatPump User Manual 165 9 Maintenance Exchanging the Active Inlet Valve AIV or its Cartridge When If internally leaking backflow Tools required Description Wrench 14mm Pair of tweezers Parts required 1 Preparations p n G5699A G1312 60025 G1312 60020 G1311 67304 0100 2298 Description Active Inlet Valve Upgrade Kit includes service and the parts listed below Active inlet valve body without cartridge Cartridge for active inlet valve 600 bar Connecting tube MCGV to AIV needed for quaternary pump only Adapter PEEK int 1 4 28 to ext 10 32 needed for isocratic pump on
121. onnections 210 Rear View of the Module 211 Interfaces 212 Overview Interfaces 215 Setting the 8 bit Configuration Switch without On board LAN 219 Communication Settings for RS 232C 220 Special Settings 222 Early Maintenance Feedback 223 Instrument Layout 224 This chapter describes the pump in more detail on hardware and electronics ot Agilent Technologies 201 12 Hardware Information The Electronics The electronics are comprised of four main components e The low pressure pump main board LPM e Power supply Optional e Interface board BCD external contacts LAN Communication Card Low Pressure Pump Main Board LPM The board controls all information and activities of all assemblies within the module The operator enters parameters changes modes and controls the module through interfaces CAN GPIB or RS 232C connected to the user interfaces The Main Power Supply Assembly The main power supply comprises a closed assembly no component level repair possibility The power supply provides all DC voltages used in the module The line voltage can vary in a range from 100 120 or 220 240 volts AC 10 and needs no manual setting Optional Interface Boards The Agilent 1200 Series modules have one optional board slot that allows to add an interface board to the modules Optional interface boards for the Agilent 1200 Series are BCD Board LAN Communication Card 202 1260 Infinity IsoPump QuatPump
122. ontrol Interface 64 Overview 64 Instrument Configuration 65 The Pump User Interface Dashboard Panel 66 Control Settings 69 Method Parameter Settings 70 Solvent Information 74 Algae Growth in HPLC Systems 80 How to Prevent and or Reduce the Algae Problem 80 Prevent Blocking of Solvent Filters 81 This chapter provides information for optimized usage of the module Apg Agilent Technologies 59 4 Using the Pump Leak and Waste Handling WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electro
123. othpick remove the frit capillary from the purge valve EE 2 Disconnect the waste tube Beware of leaking solvents PX i A due to hydrostatic pressure Valve body 2 A 3 Using the 14 mm wrench unscrew the purge valve and Zz f j remove it 4 Remove the seal cap from the purge valve PTFE frit with groove E 1260 Infinity IsoPump QuatPump User Manual 147 9 Maintenance 6 Place a new frit into the purge valve with the orientation of the frit as shown below slit in frit points to the front Reinstall the seal cap including the gold seal Before reinstallation always check the gold seal in the seal cap A deformed seal cap should be exchanged 7 Insert the purge valve into the pump head and locate the pump outlet capillary and the waste tube Outlet capillary Purge valve Waste tube 8 Tighten the purge valve and reconnect outlet capillary and waste tubing 148 1260 Infinity lsoPump QuatPump User Manual Maintenance 9 Removing the Pump Head Assembly When Tools required Preparations CAUTION Exchanging the seals Exchanging the pistons Exchanging seals of the seal wash function p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2392 Hexagonal key 4 0 mm 15 cm long T handle Switch off pump at the main power switch and unplug the power cable Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding l
124. ott Agilent Technologies 21 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 1 on page 25 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 22 1260 In
125. perate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal 1260 Infinity IsoPump QuatPump User Manual 217 12 Hardware Information Table 20 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces There is no special interface for this module 218 1260 Infinity lsoPump QuatPump User Manual Hardware Information 12 Setting the 8 bit Configuration S
126. ple if any solvent reservoir is empty this prevents system rates gt 5 mL min Min any value between 0 and the upper pressure limit setting Timetable Advanced External Contacts damage by pumping air See Timetable Settings on page 73 See Advanced Settings on page 72 The External Contacts section enables you to set up the switching of the external contacts The External Contacts section is present only when a BCD external contacts board is installed 1260 Infinity lsoPump QuatPump User Manual 71 4 Using the Pump Advanced Settings The pump advanced method setup parameters are in four sections Minimum Stroke Compressibility Maximum Flow Gradient Primary Channel Quaternary Pump only Table 10 Advanced method parameters Parameter Limits Description Minimum Stroke 20 100 pL The Stroke Volume is used for optimizing between performance of the module and seal life time For performance a low stroke volume is beneficial as it divides disturbances into smaller packages but a larger volume is extending the life time of the pump seals If Automatic is activated the pump tries to achieve an optimized stroke volume for the Jet Weaver geometry Compressibility 40 10 150 10 The compressibility of the mobile phase has an effect on the performance Default of the pump For best flow accuracy and mixing performance you can set compressibility value the parameter according to
127. ptimize the Compressibility Compensation Setting 90 1260 Infinity lsoPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 92 Status Indicators 94 Power Supply Indicator 94 Module Status Indicator 95 User Interfaces 96 Agilent Lab Advisor Software 97 This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces Apg Agilent Technologies 91 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Tests and Calibrations 92 1260 Infinity IsoPump QuatPump User Manual Troubleshooting and Diagnostics 6 System Pressure
128. r message is generated Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Wrong Pump Configuration Error ID 2060 At switch on the quaternary pump has recognized a new pump configuration The quaternary pump is assigned its configuration at the factory If the gradient valve is disconnected and the quaternary pump is rebooted the error message is generated However the pump will function as an isocratic pump in this configuration The error message reappears after each switch on Probable cause Suggested actions 1 Gradient valve disconnected Reconnect the gradient valve 112 1260 Infinity IsoPump QuatPump User Manual Error Information 7 MCGV Fuse Error ID 2043 Valve Fuse 0 Channels A and B Valve Fuse 1 Channels C and D The gradient valve in the quaternary pump has drawn excessive current causing the electronic fuse to open Probable cause Suggested actions 1 Defective gradient valve Restart the quaternary pump If the error message appears again exchange the gradient valve 2 Defective connection cable front panel to Please contact your Agilent service main board representative 3 Defective main board Please contact your Agilent service representative AIV Fuse Error ID 2044 The active
129. range Pressure pulsation Compressibility compensation Control Local control Analog output Communications Safety and maintenance Specification Dual piston in series pump with servo controlled variable stroke drive power transmission by gears and ball screws floating pistons Set points 0 001 10 mL min in 0 001 mL min increments 0 2 10 0 mL min lt 0 07 RSD or lt 0 02 min SD whatever is greater based on retention time at constant room temperature 1 or 10 pL min whatever is greater pumping degassed H30 at 10 MPa 100 bar Operating range up to 60 MPa 600 bar 8700 psi up to 5 mL min Operating range up to 20 MPa 200 bar 2950 psi up to 10 mL min lt 2 amplitude typically lt 1 3 or lt 0 3 MPa 3 bar whatever is greater at 1 mL min isopropanol at all pressures gt 1 MPa 10 bar 147 psi User selectable based on mobile phase compressibility Agilent control software e g ChemStation EZChrom OL MassHunter Agilent Instant Pilot For pressure monitoring 1 33 mV bar one output Controller area network CAN RS 232C APG remote ready start stop and shut down signals LAN optional Extensive diagnostics error detection and display through Agilent LabAdvisor leak detection safe leak handling leak output signal for shutdown of the pumping system Low voltage in major maintenance areas 1260 Infinity lsoPump QuatPump User Manual 27 2 Site Requirements and Specificat
130. rd Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 22 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 Rs 232c 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up 1260 Infinity lsoPump QuatPump User Manual Hardware Information 12 Table 23 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 24 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 25 Parity Settings without on board LAN Switches Parity 7 8 0 0 No Parity 0 1 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity 1260 Infinity lsoPump Qua
131. s and organic solvents in the quaternary pump it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the salt solution channel Regular flushing of all MCGV channels with water is recommended to remove all possible salt deposits in the valve ports Before operating the quaternary pump flush the pump and vacuum degasser see Regular Priming on page 56 This is especially recommended if it has been turned off for some time for example overnight and volatile solvent mixtures are used in the channels Prevent blocking of solvent inlet filters Never use the pump without solvent inlet filter Prevent the growth of algae see Prevent Blocking of Solvent Filters on page 81 Regularly check the purge valve frit and column frit A blocked purge valve frit can be identified by a black or yellow surface deposits or by a pressure greater than 10 bar when pumping distilled water at a rate of 5 mL min with an open purge valve When using the quaternary pump at low flow rates for example 0 2 mL min check all 1 16 inch fittings for any signs of leaks Whenever exchanging the pump seals the purge valve frit should be exchanged too When using buffers or other salt solutions flush the system with water before switching it off The seal wash option should be used when salt concentrat
132. s appropriate for the type of external equipment 1260 Infinity lsoPump QuatPump User Manual 139 9 Maintenance Overview of Maintenance and Repair The following pages describe maintenance simple repairs of the pump that can be carried out without opening the main cover Table 15 Simple Repair Procedures Procedure Typical Frequency Notes Checking and Replacing the Solvent Filter on page 142 Exchanging the Passive Inlet Valve PIV on page 143 Exchanging the Outlet Valve on page 145 Exchanging the Purge Valve Frit on page 147 Exchanging the Purge Valve Frit on page 147 Maintenance of a Pump Head Without Seal Wash Option on page 151 Exchanging pistons see Maintenance of a Pump Head Without Seal Wash Option on page 151 Exchanging the Optional Interface Board on page 164 If solvent filter is blocked If internally leaking If internally leaking If internally leaking If the frit shows indication of contamination or blockage If pump performance indicates seal wear If scratched If defective Gradient performance problems intermittent pressure fluctuations Pressure ripple unstable run Leak Rate Test for verification Pressure ripple unstable run Leak Rate Test for verification Solvent dripping out of waste outlet when valve closed A pressure drop of gt 10 bar across the frit ata water flow of 5 mL min with open purge va
133. s no multi channel gradient valve MCGV The isocratic pump does not include a degasser An upgrade product Isocratic to Quaternary Pump Upgrade Kit G4207A is available for upgrading the isocratic pump to a quaternary pump including a degasser Pump head Outlet valve Purge valve Passive inlet valve Figure 2 Overview of the Isocratic Pump 1260 Infinity lsoPump QuatPump User Manual 11 1 Introduction Overview of the Hydraulic Path 12 Both the isocratic pump and quaternary pump are based on a two channel dual piston in series design which comprises all essential functions that a solvent delivery system has to fulfill Metering of solvent and delivery to the high pressure side are performed by one pump assembly which can generate pressure up to 600 bar In the quaternary pump degassing of the solvents is done in a built in vacuum degasser Solvent compositions are generated on the low pressure side by a multi channel gradient valve MCGV which is a high speed proportioning valve The pump assembly includes a pump head with a passive inlet valve and an outlet valve A damping unit is connected between the two piston chambers A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head An optional seal wash function is available for applications using concentrated buffers as solvents 1260 Infinity lsoPump QuatPump User Manual Introduction 1 Overview of t
134. seal lifetime by flushing the back side of the seal with a wash solvent The seal wash function is strongly recommended when buffer concentrations of 0 1 M or higher will be used for long time periods in the pump The active seal wash upgrade can be ordered as G1398A The seal wash function comprises a support ring secondary seal gasket and seal holder for both piston sides Place a wash bottle filled with 90 water 10 isopropanol above the pump in the solvent cabinet The peristaltic pump moves a flow through the pump head removing all possible buffer crystals from the back of the pump seal This mixture prevents growth of algae or bacteria in the wash bottle and reduces the surface tension of the water 1260 Infinity lsoPump QuatPump User Manual Optimizing Performance 5 Choosing the Right Pump Seals The standard seal for the pump can be used for most applications However applications that use normal phase solvents for example hexane are not suited for the standard seal and require a different seal when used for a longer time in the pump For applications that use normal phase solvents for example hexane we recommend using polyethylene pump seals PE seals pack of 2 0905 1420 and Wash Seal PE 0905 1718 For normal phase applications these seals have less abrasion compared to the standard seals Polyethylene seals have a limited pressure range of 0 200 bar When used above 200 bar their lifetime is reduc
135. soPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual 11 Identifying Cables Cable Overview 188 Analog Cables 190 Remote Cables 192 BCD Cables 195 CAN Cable 197 External Contact Cable 198 Agilent Module to PC 199 Agilent 1200 Module to Printer 200 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules ott Agilent Technologies 187 11 Identifying Cables Cable Overview 188 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 35900 60750 01046 60105 Remote cables p n 03394 60600 03396 61010 5061 3378 01046 60201 BCD cables p n 03396 60560 G1351 81600 Description Agilent module to 3394 6 integrators Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description Agilent module to 3396A Series integrators 3396 Series II 3395A integrator see details in section Remote Cables on page 192 Agilent module to 3396 Series III 3395B integrators Remote Cable Agilent module to general purpose Description Agilent module to 3396 integrators Agilent module to general purpose 1260 Infinity IsoPump QuatPump User Manual Identifying Cables 11 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to mo
136. spot 100 pk Blue screw caps 100 pk Chip Column I D Assy Solvent inlet filter 20 ym pore size Capillary Fitting Starter Kit 0 17 mm id 179 10 Parts for Maintenance HPLC Starter Kit G4202 68707 180 HPLC Starter Kit incl 0 12 mm i d cap G4202 68707 p n 9301 1420 3x 9301 1450 01018 22707 5182 0716 5182 0717 5063 6507 2x 5041 2168 2x G1316 80003 5065 9937 Description Solvent bottle transparent Solvent bottle amber PTFE frits pack of 5 Screw Cap Vial 2 mL amber glass write on spot 100 pk Blue screw caps 100 pk Chip Column I D Assy Solvent inlet filter 20 um pore size Heater long down 0 12 mm i d 1 6 uL internal volume Capillary Fitting Starter Kit 0 12 mm id 1260 Infinity lsoPump QuatPump User Manual HPLC System Tool Kit Parts for Maintenance 10 The HPLC System Tool Kit G4203 68708 contains some accessories and tools needed for installation and repair of the module p n 0100 1681 0100 1710 01018 23702 5023 0240 8710 0060 8710 0510 2x 8710 0641 8710 0899 8710 1534 8710 1924 8710 2392 8710 2393 8710 2394 8710 2409 8710 2411 8710 2412 8710 2438 8710 2509 8710 2594 9301 0411 9301 1337 Description Adapter syringe seal wash tube Mounting Tool for Tubing Connections Insert tool Hex driver 4 slitted Hex key wrench 9 64 inch Wrench open 1 4 5 16 inch Hex key set 1 5 mm Pozidriv screwdriver Wrench 4 mm both ends open end Wrench open 14
137. ssor the error message is generated Probable cause 1 Disconnected or defective cable 2 Disconnected or defective connection cable front panel to main board 3 Defective active inlet valve Temperature Out of Range Error ID 2517 Suggested actions Ensure the pins of the active inlet valve connector are not damaged Ensure the connector is seated securely Please contact your Agilent service representative Exchange the active inlet valve The temperature sensor readings in the motor drive circuit are out of range The values supplied to the ADC by the hybrid sensors must be between 0 5 V and 4 3 V If the values are outside this range the error message is generated Probable cause 1 Defective main board Suggested actions Please contact your Agilent service representative 116 1260 Infinity lsoPump QuatPump User Manual Error Information 7 Temperature Limit Exceeded Error ID 2517 The temperature of one of the motor drive circuits is too high The processor continually monitors the temperature of the drive circuits on the main board If excessive current is being drawn for long periods the temperature of the circuits increases If the temperature exceeds the upper limit the error message is generated Probable cause Suggested actions 1 High friction partial mechanical blockage Remove the pump head assembly Ensure there in the pump drive assembly is no mechanical blockage of the pu
138. stem Pressure Test since LabAdvisor since LabAdvisor B 01 04 B 01 04 Leak Rate Test since LabAdvisor since LabAdvisor B 01 04 SP1 B 01 04 SP1 126 1260 Infinity IsoPump QuatPump User Manual Test Functions and Calibration 8 System Pressure Test Introduction The System Pressure Test is used for checking the tightness of the LC system and identifying leaks between the pump and a position in the flow path following the pump blocked by a blank nut System requirements Minimum software revisions Lab Advisor B 01 04 SP1 G1310B Isocratic Pump G1311B Quaternary Pump G5611A Bio inert Quaternary Pump e Lab Advisor B 02 01 G1311C Quaternary Pump VL Minimum firmware revision A 06 34 for G5611A and A 06 33 for all other pumps Test Principle A solvent can be chosen from available solvent channels and a maximum pressure can be defined at which the test will be run In contrast to older revisions of this test any solvent can be used Before the test the pump and system are flushed with solvent in order to remove air bubbles as air bubbles are compressed during the test and therefore would appear as leaks Using a degasser is highly recommended Then the flow path is blocked by a blank nut at any position between the purge valve and the TCC outlet 1260 Infinity lsoPump QuatPump User Manual 127 8 Test Functions and Calibration pressure flow leak rate time In the first phase of the test the pump
139. strumento Desconectar el aparato de la red antes de moverlo L 2J I Figure 10 Warning label illustration for correct waste tubing 44 1260 Infinity lsoPump QuatPump User Manual Installing the Pump 3 Installing the Pump Parts required Preparations p n Description 1 Pump 1 Data System and or 1 G4208A Instant Pilot 1 Power cord For other cables see text below and Cable Overview on page 188 Locate bench space Provide power connections Unpack the module Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 1260 Infinity IsoPump QuatPump User Manual 45 3 Installing the Pump Installing the Pump 1 Plac
140. t Signal Name module 1 Not connected 2 Black Analog a 3 Red Analog S e 1260 Infinity IsoPump QuatPump User Manual 191 11 Identifying Cables Remote Cables 192 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low z NC 4 Blue Shut down Low e oa NC 5 Pink Not Ls connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1260 Infinity lsoPump QuatPump User Manual Identifying Cables 11 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground ae NC 2 Brown Prepare run Low o i 3 3 Gray Start Low z NC 4 Blue Shut down Low a NC 5 Pink Not Ls connected Se NC 6 Yellow Power on
141. tPump User Manual Installing the Pump 3 Changing Solvents When Parts required Preparations CAUTION When the solvent of a channel is to be replaced by another solvent that is not compatible solvents are immiscible or one solvent contains a buffer it is necessary to follow the procedure below to prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system p n Description 1 Purging solvent s see Table 5 on page 58 1 5022 2184 Union ZDV Remove the column and replace it by a ZDV fitting Prepare bottles with appropriate intermediate solvents see Table 5 on page 58 1 If the channel is not filled with buffer proceed to step 4 Place the solvent intake filter into a bottle of water Flush the channel at a flow rate suitable for the installed tubing typically 3 5 mL min for 10 min 4 Modify the flow path of your system as required for your application For delay volume optimization see the Binary LC System User Guide Buffer salt of aqueous buffers may precipitate in residual isopropanol Capillaries and filter may be clogged by precipitating salt gt Flush solvent lines containing high concentration of salts first with water before introducing organic solvent gt Do not perform steps 5 to 7 for channels running with aqueous buffer as solvent 5 Replace the solvent bottle by a bottle of isopropanol 6 Flush the channel at a flow rate suitable for the installed
142. tPump User Manual 221 12 Hardware Information Special Settings The special settings are required for specific actions normally in a service case Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 26 Boot Resident Settings without on board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 0 0 1 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 27 Forced Cold Start Settings without on board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 0 0 0 0 0 1 222 1260 Infinity IsoPump QuatPump User Manual Hardware Information 12
143. the mobile phase being used 100 108 Maximum Flow 0 1 You can set a limit on the rate of change of the solvent flow to protect your Gradient 100 mL min min analytical column Default value 100 mL min min Primary Channel Using Automatic is recommended Quaternary pump The primary channel can be specified as A to D for optimizing highly only specific methods It is split up to deliver the first and last solvent package created by the MCGV in order to optimize composition precision The primary channel does not change during a gradient as long as the channel is used Using Automatic chooses the channel with the highest percentage at start conditions before a gradient 72 1260 Infinity IsoPump QuatPump User Manual Using the Pump 4 Timetable Settings Use the Timetable to program changes in the pump parameters during the analysis by entering a time in the Time field and appropriate values in the following fields of the timetable Changes in flow rate occur linearly from either time zero or the time of the last defined change other parameters change instantaneously at the time defined in the timetable Show Advanced Timetable toggles the timetable display between standard mode and advanced mode The following parameters can be changed Change Contacts Change Flow Change Max Pressure Limit e Change Solvent Composition You can only use solvents which have been enabled in the solvents section Function centric v
144. through about 0 2 um filters Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle Solvent bottle amber 9301 1450 supplied with the instrument for your aqueous mobile phase If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase 1260 Infinity lsoPump QuatPump User Manual Using the Pump 4 Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module e Use a sterile if possible amber solvent bottle to slow down algae growth e Filter solvents through filters or membranes that remove algae Exchange solvents every two days or refilter e If the application permits add 0 0001 0 001 M sodium azide to the solvent e Place a layer of argon on top of your solvent Avoid exposure of the solvent bottle to direct sunlight Never use the system without solvent filter installed 1260 Infinity lsoPump QuatPump User Manual 81 4 Using the Pump Prevent Blocking of Solvent Filters 82 1260 Infinity IsoPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual
145. tions and Calibration Leak Rate Test 136 1260 Infinity IsoPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual 9 Maintenance Introduction to Maintenance and Repair 138 Warnings and Cautions 139 Overview of Maintenance and Repair 140 Cleaning the Module 141 Checking and Replacing the Solvent Filter 142 Exchanging the Passive Inlet Valve PIV 143 Exchanging the Outlet Valve 145 Exchanging the Purge Valve Frit 147 Removing the Pump Head Assembly 149 Maintenance of a Pump Head Without Seal Wash Option 151 Maintenance of a Pump Head with Seal Wash Option 154 Reinstalling the Pump Head Assembly 158 Seal Wear in Procedure 160 Exchanging the Multi Channel Gradient Valve MCGV 161 Exchanging the Optional Interface Board 164 Exchanging the Active Inlet Valve AIV or its Cartridge 166 Exchanging the Seal Wash Cartridge 168 Replacing the Module Firmware 170 This chapter describes the maintenance of the module ste Agilent Technologies 137 9 Maintenance Introduction to Maintenance and Repair The module is designed for easy repair The most frequent repairs such as piston seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack These repairs are described in Overview of Maintenance and Repair on page 140 138 1260 Infinity IsoPump QuatPump User Manual Maintenance 9 Warnings and Cautions Toxic flammable and hazardous sol
146. tubing typically 3 5 mL min for 5 min 7 Swap the bottle of isopropanol with a bottle of solvent for your application 8 Repeat steps 1 to 7 for the other channel s of the pump 9 Install the desired column set the required composition and flow rate for your application and equilibrate the system for approx 10 minutes prior to starting a run 1260 Infinity IsoPump QuatPump User Manual 57 3 58 Installing the Pump Table5 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation lsopropanol Best solvent to flush air out of When switching between lsopropanol the system reverse phase and normal Miscible with almost all phase both times solvents After an installation To clean the system when using buffers After changing aqueous solvents After the installation of normal phase seals PE seals pack of 2 0905 1420 Ethanol or methanol HPLC grade water HPLC grade water Hexane 5 isopropanol Alternative to isopropanol second choice if no isopropanol is available Best solvent to re dissolve buffer crystals Best solvent to re dissolve buffer crystals Good wetting properties 1260 Infinity IsoPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual 4 Using the Pump Leak and Waste Handling 60 Hints for Successful Use of the Pump 61 Setting up the Pump with the G4208A Instant Pilot 63 Setting up the Pump with the Instrument C
147. ump User Manual Error Information 7 Pump Head Missing Error ID 2202 2212 The pump head end stop in the pump was not found When the pump restarts the metering drive moves forward to the mechanical end stop Normally the end stop is reached within 20 s indicated by an increase in motor current If the end point is not found within 20 s the error message is generated Probable cause Suggested actions 1 Pump head not installed correctly screws Install the pump head correctly Ensure nothing not secured or pump head not seated e g capillary is trapped between the pump correctly head and body 2 Broken piston Exchange the piston Index Limit Error ID 2203 2213 The time required by the piston to reach the encoder index position was too short pump During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is reached too fast the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative 1260 Infinity IsoPump QuatPump User Manual 119 7 120 Error Information Index Adjustment
148. ure to release the pressure by slowly opening the purge valve when the test has finished Evaluating the Results on page 130 describes the evaluation and interpretation of the System Pressure Test results For detailed instructions refer to the Agilent Lab Advisor software 1260 Infinity lsoPump QuatPump User Manual 129 130 Test Functions and Calibration Evaluating the Results The test fails if the leak rate between pump and blank nut is higher than the limit of 3 uL min If the System Pressure Test fails Ensure that all fittings between the pump and the blank nut are tight Repeat the test Often it is only a damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened If the test fails again insert the blank nut at the outlet of the previous module in the stack e g autosampler outlet if TCC has been tested before and repeat the test Exclude each module one by one to determine which module is leaking If the pump is determined to be the source of the leak run the Pump Leak Rate Test 1260 Infinity IsoPump QuatPump User Manual Test Functions and Calibration 8 Potential Causes of System Pressure Test Failure System Pressure Test failed The test will fail if the sum of all leaks in the system pump autosampler or column co
149. vement of piston 2 secondary leak the following reasons are possible Probable cause Suggested actions 1 System not flushed properly Flush system for several minutes 2 Degassing efficiency is low Check degasser performance 3 Purge valve not closed or defect Check purge valve 4 Blank nut not installed tightly Tighten or replace blank nut 5 Outlet valve leaking read below Replace outlet valve 6 Leak at piston 2 or seal in chamber 2 Inspect piston replace piston and or seal Primary Leak If a leak is found for movement of piston 1 primary leak any leak described for piston movement 2 will cause a failure for piston 1 as well as the liquid can move through the outlet valve to chamber 2 Such cases need to be identified as described before Additionally following causes are possible Probable cause Suggested actions 1 Leak at piston 1 or seal in chamber 1 Inspect piston replace piston and or seal 2 Leak at inlet valve Replace inlet valve or inlet valve cartridge AIV only Internal Outlet Valve Leak A leak of the outlet valve will be identified separately internal outlet valve leak by calculating the difference between leak rate 1 and leak rate 2 If the second leak rate is higher than the first one this is due to a flow back through the outlet valve Probable cause Suggested actions 1 Leak at outlet valve Replace the part which has failed and re run the test 1260 Infinity lsoPump QuatPump User Manual 135 8 Test Func
150. vents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standard
151. voir Rad i bs B pee To waste container 1260 Infinity IsoPump QuatPump User Manual 169 9 Maintenance Replacing the Module Firmware When Tools required OR OR Parts required Preparations 170 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module 1260 Infinity lsoPump QuatPump User Manual 1260 Infinity lsoPump QuatPump User Manual 10 Parts
152. witch without On board LAN The 8 bit configuration switch is located at the rear of the module This module does not have its own on board LAN interface It can be controlled through the LAN interface of another module and a CAN connection to that module i SS Y Figure 29 Configuration switch settings depend on configured mode All modules without on board LAN default should be ALL DIPS DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 DIP 1 DOWN and DIP 2 UP allows special RS 232 settings e for boot test modes DIPS 1 2 must be UP plus required mode For normal operation use the default best settings Switch settings provide configuration parameters for serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN 1260 Infinity lsoPump QuatPump User Manual 219 12 Hardware Information 220 The following tables represent the configuration switch settings for the modules without on board LAN only Table 21 8 bit Configuration Switch without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 RSVD SYS RSVD RSVD FC The LAN settings are done on the LAN Interface Card G1369B C Refer to the documentation provided with the ca
153. ydrostatic pressure If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube 1 Remove the solvent filter from the inlet filter adapter and replace it by a new one 1260 Infinity IsoPump QuatPump User Manual Maintenance 9 Exchanging the Passive Inlet Valve PIV When If internally leaking backflow Tools required Description Wrench 14mm Pair of tweezers Parts required p n G1312 60066 Description Preparations Remove the front cover Passive inlet valve 1220 1260 1 Disconnect the solvent inlet tube from the inlet valve be aware that solvent may leak out of the tube due to hydrostatic flow Passive inlet valve Solvent inlet tube 2 Using a 14 mm wrench loosen the passive inlet valve and remove the valve from the pump head 1260 Infinity lsoPump QuatPump User Manual 143 9 Maintenance 3 Insert the new valve into the pump head and tighten the valve using a torque wrench 12 Nm Next Steps 4 Reconnect the solvent inlet tube to the passive inlet valve 5 Reinstall the front cover 144 1260 Infinity IsoPump QuatPump User Manual Exchanging the Outlet Valve Maintenance 9 When If internally leaking Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 1924 Wrench open 14 mm Parts required p n Description G1312 60067 Outlet valve 1220 1260 Preparations Switch off pump

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