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User manual English Series EuroFlow, Type EF Type EF/B

1. 5 6 Power save mode When not using the cabinet for a short period of time put it in the power save mode The fan will run at half speed to save energy synonyms for power save mode are standby mode or night mode Power save mode y e Push button a When the cabinet is locked enter the user code The fan runs at half speed Cabinet in power save mode not safe There is an acoustic alarm Downflow to low Inflow MENU Socket Off e To acknowledge this alarm push button A Slide down the window onto the aerofoil to cease the alarm Switch off procedure y P e With the next push on the power save button q the cabinet enters the switch off procedure Cabinet switched off Not Safe 3 Monday 11 06 33 MENU Socket Of f Work mode l e Push button and slide the window back in working mode 4 Cabinet in work mode Safe Monday 11 06 33 MENU Socket Of f 5 7 Turn off Remove all materials en accessories from the working area Remove all spilled liquids and other parts in a responsible manner according to the regulations UM EFAB EN10 doc 28 M Clean An Telstar EuroFlow EF and EF B Biological Safety Cabinet lt T The alarm indicator becomes red and there is an acoustic alarm e Slide the sliding window on to the aerofoil Cabinet in work mode not safe Window is not in the working position MENU Socket Of f e
2. UM EFAB EN10 doc 40 EuroFlow EF and EF B Biological Safety Cabinet Cm Netstar Status information cabinet shows see example e Middle numbers are the actual air speeds yellow e Left and right numbers are the set up alarm borders red Information fan shows e Push button MEN gt Information fan setting 0 1000 1110011 jii ij E 0 Power save 350 Work mode 520 Actual SON gt The seven digit number in the upper right corner is only meant for service aims e Push button B Esc for starting menu xl The average inflow velocity at the top of the front aperture is measured and can be read in the display The minimal required value of the average inflow velocity measured at the top of the front aperture is given in chapter 9 2 on page 61 Measuring these most critical measured values directly under the window ensures that the overall average inflow velocity is according to the guidelines of the EN12469 2000 Annex H table H1 Mean inflow velocity to achieve operator protection gt 0 4 m s Information about the installed options The display only shows when the options are installed Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Cabinet in work mode Safe Monday 11 06 33 MENU Socket 0f f pou e Push button EM Menu 1 A Hour counter information B gt Status cabinet C Settings A 2 Information about the installed opt
3. Cabinet in work mode Cabinet in work mode Safe See 5 4 Starting up the cabinet Monday 11 06 33 1 on page 26 MENU Socket Of f Factory adjustments e Push button KB Menu e Push button n C e Push 2x button En gt e Push button e Enter the code for Factory adjustment authorized personnel only Factory adjustment 0000 Enter code Factory adjustment display shows Factory settings can be changed here authorized personnel only See service Factory adjustments manual for more information Version X X XXxxxx 10 VX XX OPS Wake 2 Version X X version of Display A Enter board I 0 VX XX version of IO l Bee board OPT VX X version option board e Push button Esc for starting menu UM EFAB EN10 doc 44 EuroFlow EF and EF B Biological Safety Cabinet Testing of alarms Clean A Netstar For service purposes only More information in Appendix VII Alarm test procedure on page 79 Cabinet in work mode See 5 4 Starting up the cabinet 1 on page 26 2 Testing of alarms display shows Cabinet in work mode Safe Monday 11 06 33 MENU Socket Of f e Push button EB and EB 0 e Push 3x button SEB e Push button A Testing of alarms 0000 Enter code e Enter service code 3 For service purposes only The various values of the fan and velocities are shown on the display In case of interferen
4. Installed options microprocessor This chapter describes the codes from 5 13 Information about the installed options on page 41 Menu B from the microprocessor e Startupin Menu Display e Push button GS Menu e Push button D B e Push button n for screen I e Push button ED gt for screen II e Push button NE gt for screen III Screen I Installed options Option Options No Electrical window E 0 Figure 46 Example of screen I Explanation of the codes from the menu e E0 no electrical window e E1 electrical window no functionality for fan not available for EF en EF B e E2 work mode if cabinet is switched on e C3 relay K2 on option print UM EFAB EN10 doc 84 e NI Pe A Clean A Telstar EuroFlow EF and EF B Biological Safety Cabinet Screen II Installed options relays Installed Options Relais Al Relais B1 Figure 47 Example of screen IT Overview functions of relay K8 A1 A12 Alarm A1 A12 relay switching K8 on output J5 at the IO print Set up from A1 until A12 A1 Relay ON ZA amp HD LD HI LI DEFAULT A2 Relay ON A8 HD LD HI LI R A3 Relay ON W amp HD LD HI LI A4 Relay ON W amp HD LD HI LI R A5 Relay ON W amp HD LD A6 Relay ON A8 HD LD A7 Relay OFF ZA amp HD LD HI LI A8 Relay OFF A amp HD LD HI LI R A9 Relay OFF W amp HD LD HI LI A10 Relay OFF
5. EN 12469 Annex A 10 EN 12469 Annex A 10 EN 12469 Annex A 11 EN 12469 Annex B EN 12469 Annex C EN 12469 Annex D EN 12469 Annex E EN 12469 Annex F EN 12469 Annex G EN 12469 Annex H Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Installation test Yes Type test Yes Type test Yes Installation test Yes Type test Yes UM EFAB EN10 doc 89 EuroFlow EF and EF B Biological Safety Cabinet Clean Maus Overview of the required specifications according to DIN 12980 2005 Requirement shortened According to Complied Lighting Sound level Vibration Stability Materials general Materials general Materials general Glass and front windows Electrical safety Ergonomics Ergonomics Temperature Leakage of carcass Retention efficiency Aerosol challenge method Product protection Cross contamination Volumetric airflow rate Design and airflow velocities At least 800 lux 60 dB A according to DIN 45635 1 0 005 mm RMS at centre workspace Between 20 and 20000 Hz Conform to DIN EN 61010 1 2002 08 and EN 12100 1 2003 and 12100 2 2003 The material likely to be in contact with cytotoxics should b
6. Replacement of the downflow HEPA filter For replacement of the downflow HEPA filter the following procedure must be followed Supplies needed Plastic bag with closure minimum measurement depends on the volume of the HEPA filter Two pairs of gloves Semi facial mask with HEPA filters Tools knife spanner or socket wrench alien key Filter exchange set Tape Chemical disposal container or chemical disposal procedure Removing the old downflow HEPA filter Decontaminate the cabinet Switch off the installation and plug out the power plug Put on the gloves and keep an extra pair ready for use Put on your breathe protection e g with a semi facial mask with HEPA filters Remove the aerofoil Put the window in its disinfection position Open the top hood Remove the safety plate for the electronics Remove the front panel remove the screws Unloose the bolts of the pressure bars and remove the bars Position the Filter change set Lifting up the plenum step by step Loosen the HEPA filter Take HEPA filter out of the cabinet and put it into an appropriate plastic bag Seal the plastic bag airtight Remove the gloves and put them in the appropriate container UM EFAB EN10 doc 70 EuroFlow EF amp EF B Biological Safety Cabinet Clean Air Techniek B V Clean Ai Position the new downflow HEPA filter Unpack the new filter check specifications and register the serial number and specifications Filter
7. Press the air out of the bag with its opening still in the working space Seal the plastic bag airtight Remove the plastic bag and place it in an appropriate container Remove the second HEPA filter and put it in an appropriate plastic bag Fold back the edge of the plastic bag not touching the inner side of the plastic bag Press the air out of the bag with its opening still in the working space Seal the plastic bag airtight Remove the plastic bag and place it in an appropriate container Remove the gloves and put them in the appropriate container Position the new 1 HEPA filter section of a pressure frame Put on new gloves Place two new HEPA filters Take care that the top side of the filters are level with the top side of the filter casing If a filter is lower then tape should be applied to the bottom of the filter to make sure that the topsides of both filter and filter casing are level Place the pressure frame back Start again with procedure for Removing the old 1st HEPA filter sections of a pressure frame on page 73 with the next 1 HEPA filters Place the pre filter wire mesh the pre filter itself and the work top back Integrity test Perform an Integrity test according to the procedure described in Appendix IV Efficiency test of the HEPA filters on page 74 UM EFAB EN10 doc 73 M M a Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet Appendix I V Efficiency test of the HEPA filter
8. Top hood is also open Disinfection position Aerofoil is removed window in lowest position Savings UV hinge position Window is closed on the aerofoil Working position Safe work opening Workposition calibrated UM EFAB EN10 doc 22 Clean M ass EuroFlow EF and EF B Biological Safety Cabinet Top position Maximum opening to place objects in cabinet Figure 8 Window positions Slide the window To slide the window both handles need to be used Note 1 When the window is not in the working position the safety of the cabinet cannot be granted This status will cause an alarm Hinge the window The window construction can be hinged manually for example when the working area and window need to be cleaned but also for placing large objects in the working area The window can only be hinged when the window is slit downwards on the aerofoil and when the locking pins are locked see Figure 12 and the swivel blocks are opened see Figure 15 Hinge the window open and closed e Open the top hood up see Figure 9 Pos 1 e Hinge both the side profiles open see Figure 9 Pos 2 e Turn the locking pins which are mounted on the plastic window slides 90 degrees see Figure 12 e Slide the window downwards see Figure 9 Pos 3 until it clicks in low position light hoo 2 Turn the side profiles outwands 3 Close sliding window i 4 Swivel block Figure 9 Hinge instructions e Turn b
9. EN 1822 EN 1822 EN 1822 EN 1822 First HEPA filter HEPA V pleats HEPA V pleats Type PB H14 Type PB H14 H14 acc EN H14 acc EN 1822 8 pieces 1822 12 pieces Performance EF 4 EF 5 EF 6 EF B4 EF B 6 Sound level dB A according to ISO 57 60 56 60 60 11201 m s Average downflow speed 0 36 0 05 0 36 0 05 0 36 0 05 0 36 0 05 0 36 0 05 in work mode m s Average downflow speed 0 10 0 20 0 10 0 20 0 10 0 20 0 10 0 20 0 10 0 20 in standby mode Average inflow speed in m s top of front aperture in 0 28 0 28 0 28 0 28 0 28 work mode Average inflow speed in m s whole front aperture in 20 5 gt 0 5 gt 0 59 gt 0 5 gt 0 5 work mode i m h Exhaust airflow 400 25 500 25 600 25 400 25 600 25 work mode xl As where no individual measurement should differ more than 2096 from the mean us The average inflow velocity measured at the top of the front aperture should be at least 0 28 m s the overall average inflow velocity should be at least 0 40 m s EN 12469 2000 UM EFAB EN10 doc 62 EuroFlow EF amp EF B Biological Safety Cabinet Clean Air Techniek B V Clean Ai EF 4 EF 5 EF 6 EF B4 EF B6 Electrics Power connection V 230 5 230 5 230 5 230 5 230 5 Hz 50 50 50 50 50 i A Needed main fuse box T i i
10. Lighting and UV cannot be switched on both at the same time As a safety precaution one of them will automatically switch off 5 9 Control gas supply optional The gas function is only visible if this option is installed and the cabinet is in work mode with no air alarms In all other cases the gas valve will automatically be switched off and the gas function is invisible The gas valve which controls the gas supply can only be switched on in the work mode and when there are no airflow alarms this is not possible in the start mode Switching gas supply on Cabinet in work mode See 5 4 Starting up the cabinet 1 07 page 26 Cabinet in work mode Safe Monday 11 06 33 MENU Socket Of f Gas Of f n n pou The display shows pasen e Push button Gas off The gas valve will be switched on Cabinet in work mode Safe 2 Monday 11 06 33 Gas switched on MENU Socket Of f 3 Turn the gas tap open close Switching gas supply off The display shows Gas Off SB Gas 4 The gas valve will be switched Push button Gas On on UM EFAB EN10 doc 30 EuroFlow EF and EF B Biological Safety Cabinet Clean A NA ferstar Cabinet switched off Not Safe Microbiological Safety Cabinet Gas switched off MENU Socket Of f Gas Of f 5 10 Control sockets The sockets can always be switched on and off If the cabinet is switched off
11. UM EFAB EN10 doc 85 EuroFlow EF and EF B Biological Safety Cabinet Screen III Analoque inputs only if installed Analog inputs ADI 5 0 C AD2 1 0 pM Figure 48 Example of screen III Analogue inputs can be used to program alarm settings for e g temperature C of working surface temperature C of work area particle counters ppm relative humidity pressure Pa in plenum If alarm limits are exceeded an alarm will sound as short beeps with intervals Clean A Ne 3 Telstar To install alarm limits start from the menu as described in 5 13 Information about the installed options on page 41 The display only shows when the options are installed Starting from screen III Analog inputs ADI 5 0 C AD2 1 0 pM gt 2 Settings for High and Low Alarm e Push button HEB gt go back to the starting menu Analog inputs Change ADI L ADI 5 0 H 0 0 AD2 1 0 pM a e t e Choose the required limits L Low H High for AD1 and AD2 using button EB ano EB e Push button KE Esc to confirm the settings and to An alarm can be transferred depending on the code B5 through relay K3 C5 through relay K2 option print UM EFAB EN10 doc 86 EuroFlow EF amp EF B Biological Safety Cabinet Appendix X User code Activating the user code Clean Air Techniek B V lt Clean The function User code Active not act
12. Version 60 Standby mode 28 Starting up the cabinet 26 UM EFAB EN10 doc Clean Wits Statement of the agreement for machines 95 Stickers 81 Support frame 16 I Technical specifications 61 Test aerosol 74 Testing of alarms 45 Timer 34 Top hood 15 Turn off 28 TUV 82 Type plate 14 U Using the cabinet 22 UV light 20 UV off 30 UV on 29 V Vacuum connection 19 W Work opening 6 7 Worktop 15 98
13. and 20000 Hz Conform to DIN EN 61010 1 2002 08 and EN 12100 1 2003 and 12100 2 2003 The material likely to be in contact with micro organisms should be uniformly corrosion resistant non flammable and non absorbing Materials and sealants for joints should be durable and resistant to cleaning and disinfection agents and resistant to general use of MSCs Manufacturers should ensure that the materials of construction will not be damaged by fumigation with formaldehyde Laminated safety glass or UV resistant safety plastics Conform to EN 12100 1 2003 and 12100 2 2003 Conform to EN 61010 1 Conform to EN 12100 1 2003 and 12100 2 2003 Gas supply should be controlled by a suitable valve that can be opened when the cabinet is running and that closes under any other conditions Maintenance can be carried out safely on the cabinet after installation Considered according to prEN ISO 14738 1997 Air temperature in work space 8 above ambient laboratory temperature No visible leakage with soap bubble test Microbiological test method or potassium iodide KT method Particle counter 0 0596 Aerosol photometer 0 0190 300 CFU of B subtilis EN 12469 Annex A 1 EN 12469 Annex A 2 EN 12469 Annex A 3 EN 12469 Annex A 3 EN 12469 Annex A 4 EN 12469 Annex A 5 EN 12469 Annex A 6 EN 12469 Annex A 6 EN 12469 Annex A 6 EN 12469 Annex A 7 EN 12469 Annex A 7 EN 12469 Annex A 8 EN 12469 Annex A 9 EN 12469 Annex A 9
14. certificate has to be stored on a safe place Put Vaseline on the seal of the HEPA filter both sides Position the HEPA filter take care for possible damage of the sealings Reposition the plenum downwards and remove the filter exchange set Reposition the pressure bars and fasten the bolts Reposition the front panel Reposition the safety plate for the electronics Put off your gloves and put these in an appropriate bag Hinge the top hood down Put the aerofoil back in position Put the window into working position Place the power plug in the supply socket and switch on the installation Perform an Integrity test according to the procedure described in Appendix IV Efficiency test of the HEPA filters on page 74 Replacement of the exhaust HEPA filter For replacement of the exhaust HEPA filter the following procedure must be followed Supplies needed Plastic bag with closure minimum measurement depends on the volume of the HEPA filter Two pairs of gloves Semi facial mask with HEPA filters Tools knife spanner or socket wrench alien key Tape Chemical disposal container or chemical disposal procedure Removing the old exhaust HEPA filter Decontaminate the cabinet Switch off the installation and plug out the power plug Put on the gloves and keep an extra pair ready for use Put on your breathe protection e g with a semi facial mask with HEPA filters In case the cabinet is connected to an externa
15. gt e Push button A User code 0000 Enter code e Enter actual user code e Push button f Input user code New code 0000 0001 Old code gt gt e Enter the new valid user code e Push button A The pre installed valid user code is 0001 In case of emergency there is a special code to break the unknown or forgotten user code In that case enter code 2882 and the original pre installed code 0001 is valid again UM EFAB EN10 doc 88 EuroFlow EF and EF B Biological Safety Cabinet Appendix XI Required specifications Clean Maus Overview of the required specifications according to EN 12469 2000 Requirement shortened According to Complied Front aperture height Lighting Sound level Sound level Buzzer Vibration Stability Materials general Materials general Materials general Glass and front windows Glass and front windows Electrical safety Gas supply optional safety Gas supply optional safety Ergonomics Ergonomics Temperature Leakage of carcass Retention efficiency Aerosol challenge method Product protection Cross contamination Volumetric airflow rate Design and airflow velocities Between 160 and 250 mm At least 750 lux at work surface 65 dB A when background level is 55 dB A according to ISO11201 Exceed 10 dB A more than installation sound pressure 0 005 mm RMS at centre workspace Between 20
16. is then drawn through a pre filter B by a fan E This fan blows the air in the correct proportion to the downflow HEPA filter D and the exhaust HEPA filter F The extracted air of the cabinet can recirculate into the laboratory or can be blown into an exhaust channel UM EFAB EN10 doc A AAA ANN DIS 7 W C A l VNNANVNIVNVA o dj TA DOWNFLOW INFLOW i a No Figure 1 Sectional view EF EuroFlow EF and EF B Biological Safety Cabinet 1 2 EF B Clean A pO The EF B is a class II Biological Safety Cabinet It gives the user the possibility to work safely and sterile with bioagentia without complete physical barrier between person and experiment through work opening C Working surface A is kept clear of particles by means of a clean downflow of air Because of the downflow air passed through a HEPA filter High Efficiency Particulate Air the working area of the cabinet is free of particles This airflow is laminar the airflow has got no turbulence and has got a constant air speed Through the work opening C air is drawn into the workspace inflow towards the front slots of the worktop This protects the personnel against pathogenic aerosols that might be released The downflow and inflow air is then drawn through a pre filter B and a V shaped HEPA filter G by a fan E mounted on a plenum construction This fan blows the air in the correct proportion to th
17. located outside the installation can be assembled by customer request in top of the installation For EF B series assembly of this connection on the bottom is not possible Vacuum connection This connection is always assembled in combination with a vacuum tap Positions of the supply connection EF e The connection that is located inside the installation is standard assembled in combination with a vacuum tap in the back panel of the workspace UM EFAB EN10 doc 19 Clean pO EuroFlow EF and EF B Biological Safety Cabinet The connection that is located outside the installation is standard assembled in the bottom of the installation The connection that is located outside the installation can be assembled by customer request in left or right side panel or top of the installation Positions of the supply connection EF B The connection that is located inside the installation is standard assembled in combination with a vacuum tap in the back panel of the workspace The connection that is located outside the installation is standard assembled in the left or right side panel of the installation The connection that is located outside the installation can be assembled by customer request in top of the installation For EF B series assembly of this connection on the bottom is not possible UV light The UV light is meant for disinfection of the working surface of the safety cabinet When the UV light switches on the FL li
18. of the working surface Window on lowest window position When the window is slid down in its lowest position and the aerofoil is removed this message will be on the display 6 2 Flow alarms If one of the following alarms occurs and no apparent cause can be found the service department has to be contacted They will ask for the status of the cabinet which helps to find possible causes see 5 13 Information status cabinet on page 40 Downflow too low Downflow too low Figure 20 Product protecting downflow is too low Product protection becomes less there is a chance that aerosols from the outside go into the working area Person protection stays intact Downflow too high Downflow too high Figure 21 Product protecting downflow is too high Product protection stays intact Person protection becomes less because the collision of particles against the personnel s arms increases Aerosols can escape through the air curtain to the outside Inflow too low Inflow too low Figure 22 Product protection stays intact Person protection becomes less aerosols from the work area can go outside the cabinet because of a weakened air curtain UM EFAB EN10 doc 48 Pa ektar EuroFlow EF and EF B Biological Safety Cabinet a Inflow too high Inflow too high Figure 23 Person protection stays intact Product protection becomes less The inflow air through the work opening has a higher speed and may pass the fron
19. the 185 nm ozone forming line A protective coating on the inside limits the depreciation of the useful UV C radiation output Longlife lamps PL S have a specially adapted starter providing almost instant starting characteristics already built into the lamp base Note The UV C radiation output of these lamps is indicated by the following warning signs DANGER UV C RADIATION PROTECT EYES Dimensions n mm Type g 2 Lamp voltage v TUV 4W TUV ew TUV OW TUV 11w TUV 16W 8 42 56 37 4 AAAS tap aji inj in TUV 10W 45 TUV 15W 51 TUV 25W 46 TUV 30W TUV J W TUV 55W TUV 75W HO HO TUV 115W TUV 115W VHO RVHO TUV PLS TUV SW TUV IW TUV 11W TUY PL L TUV 1BW Tuv 36w TUV 55W HF PLS PLS PL S PLL PLL PLL TUV 25317 23130 3655 4047 4358 546 1 Wavelength in nm 5780 Spectral power distribution UM EFAB EN10 doc Lamp current A Disinfection Applications Philips TUV lamps are used for killing or inactivating bacteria viruses and other primitive organisms Typical application examples include air water and surface disinfection in hospitals bacteriological research and pharmaceutical institutions and food processing industries such as dairies breweries and bakeries They are also used for the disinfection of drinking water waste water swimming pools air conditioning systems cold storage rooms packing material etc Finally they are applied in a variety of pho
20. up or down and opened and closed by means of handgrips The window construction contains two guiding profiles with a compression spring The spring will push a ball into a hole in the guiding shaft when the window enters the working position this working position is also indicated by means of two blue indication stickers The window can be hinged but only in the hinge position the top hood has to be opened first See 5 2 Top hood sliding window on page 23 The hinge option offers the advantage that even larger objects can be placed inside the working space It also makes cleaning the working space as well as the inside of the window easier see 7 1 Cleaning the installation on page 51 4 5 Top hood In and behind the top hood Figure 3 Figure 4 Figure 5 and Figure 6 Pos 5 a number of electrical components are assembled e FL lighting e Operating panel operating board e Interface board including engine control e Additional option print If you wish to check one of these components you need to hinge the top hood To check the interface board the safety cover has to be taken away authorized service personnel only Caution Some of the components are live and therefore carry a risk of electrical shock as long as the mains plug is connected 4 6 Worktop The workroom contains a stainless steel type 304 worktop Figure 3 Figure 4 Figure 5 and Figure 6 Pos 6 with a deepened work surface This deepened work surface is call
21. 0 max xt The average inflow velocity at the top of the front aperture is measured and can be read in the display The minimal required value of the average inflow velocity measured at the top of the front aperture is given in chapter 9 2 on page 61 Measuring these most critical measured values directly under the window ensures that the overall average inflow velocity is according to the guidelines of the EN12469 2000 Annex H table H1 Mean inflow velocity to achieve operator protection 0 4 m s UM EFAB EN10 doc 80 e M Clean A Telstar EuroFlow EF and EF B Biological Safety Cabinet Appendix VIII Stickers 1 Sticker cabinet type This sticker can be found on the outside underneath the cabinet For an example of the sticker see the picture underneath Clean Air Techniek bv Kuipersweg 37 NL 3449 JA Woerden Ihe Netherlands t 31 0 348 411114 f 31 0 348 422684 e info icleanairbv nl Clean Ai Ir W i www cleanairbv com Cle pan Air Techniek BV BM i A Type EF A6 Voltage 230V 50Hz Class Class II Current 4 Amp Project nr Power 0 92 KW Serial nr Fan ZIEHL ABEGG Constr Date 28 07 2008 Exhaust fan Standards EN 12469 First HEPA filter Year Standard 2000 Downflow filter 18 P6 Work place 44x171cm Exhaust filter 6P7 Inflow filter C Pre filter 44 5x176cm 2 Sticker filter type This sticker can be found behind the top hood and on top of the cabinet for downflow
22. 00 Di sable Change Disable e Push button BEI Disable to reset all previous 2 settings Change days e Push button BE Change to change the day Tuesday Change day Cabinet to work mode at 0 00 3 e The value can be changed by pushing BEI or EB Change hours TY a e Push button MEM gt gt to change the time UM EFAB EN10 doc 38 EuroFlow EF and EF B Biological Safety Cabinet Poen ac Netstar Change hours Cabinet to work mode at 10 00 e The value can be changed by pushing button Ex or Ew Change minutes Thursday Cabinet to work mode at 10 00 5 Change minutes Cabinet to work mode at 10 30 The value can be changed by pushing button EB or EEB Acknowledge the setting 6 Acknowledge the setting with button A Push 2x button EN Esc for starting menu For more days hours minutes walk again through the menu and confirm each time Set up actual time Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Cabinet in work mode Safe Monday 11 06 33 MENU Socket Of f Set up actual time Set up display the actual day shows e Push 6x button ME ix Menu 1 x A and 4x gt Set up actual time Monday 11 06 33 Set up actual time change day Monday 11 06 33 pu e Push button ME UM EFAB EN10 doc 39 Pe A Clean A Telstar EuroFlow EF and EF B Biologica
23. 297 2297 i 1552 745 1552 745 1552 745 1552 745 1552 745 900 mm without exhaust hood ae mm Interior dimensions 1190x605x725 1495x605x725 1800x605x725 1190x605x725 1800x605x725 Ixdxh Work area Ixd mm 1109x440 1414x440 1719x440 1109x440 1719x440 Mass without frame ka 255 275 315 300 380 Exhaust connection mm 250 250 250 250 250 Window 8 mm laminated glass 2 layers 8 mm laminated glass 2 layers 8 mm laminated glass 2 layers 8 mm laminated glass 2 layers 8 mm laminated glass 2 layers UM EFAB EN10 doc 61 EuroFlow EF and EF B Biological Safety Cabinet Waste Clean Filter EF 4 EF5 EF 6 EF B 4 EF B 6 Pre filter mm G3 EN 779 G3 EN 779 G3 EN 779 G3 EN 779 G3 EN 779 1150x445 1460x445 1760x445 1150x445 1760x445 Downflow filter mm Astrocell Astrocell Astrocell Astrocell Astrocell HEPA 12P6 HEPA15P6 HEPA 18P6 HEPA 12P6 HEPA 18P6 610x1220x69 610X1524X69 610x1830x69 610x1220x69 610x1830x69 mm H14 acc mm Hi4 acc mm H14 acc mm Hi4 acc mm H14 acc EN 1822 EN 1822 EN 1822 EN 1822 EN 1822 Exhaust filter mm Astrocell Astrocell Astrocell Astrocell Astrocell HEPA 4P4 HEPA 6P6 HEPA 7P6 HEPA 4P6 HEPA 7P6 457X457X69 610X610X69 762x610x69 457x610x69 762x610x69 mm Hi4 acc mm H14 acc mm Hi4 acc mm Hi4 acc mm H14 acc EN 1822
24. 2W amp HD LD HI LI R A11 Relay OFF W amp HD LD A12 Relay OFFZ A amp HD LD Explanation codes used above W Working mode S Save mode A Cabinet on and spare mode HD High downflow alarm LD Low downflow alarm HI High inflow alarm LI 2 Low inflow alarm R Window alarm amp AND function OR function Functions are factory adjustments and cannot be changed by the user Overview functions of relay K3 B1 B5 Alarm B1 B5 relay switching K3 on output J22 at the IO print e B1 the K3 relay is activated if the main fan is switched on working mode saving mode 3 B2 the K3 relay is activated if the main fan is on half speed mode saving mode 3 B3 the K3 relay is activated if the main fan is in the working mode 3 B4 the K3 relay is activated if the option Disinfection in the menu is chosen 3 B5 the K3 relay is activated if there is an alarm from the analogue inputs Overview functions of relay K2 C1 C5 Alarm C1 C5 relay switching K2 on output J14 at the Option print e C1 the K2 relay is activated if the main fan is switched on working mode saving mode C2 the K2 relay is activated if the main fan is on half speed mode saving mode 3 C3 the K2 relay is activated if the main fan is in the working mode C4 the K2 relay is activated if in the menu is chosen for the option Disinfection C5 the K2 relay is activated if there is an alarm from the analogue inputs
25. 6 Put on gloves Clean the surface with the SDS solution on a tissue Natriumdodecylsulfat solution Repeat this action with a new tissue Clean the surface with a moist cloth Repeat this action Dry the surface with a paper tissue Cleaning the aerofoil BI OLOGI CAL DANGER Never take the aerofoil outside a used cabinet without precaution for instance disinfection UM EFAB EN10 doc 51 Clean Ac 7 Nan Ist EuroFlow EF and EF B Biological Safety Cabinet ee Put on gloves Clean the top side of the aerofoil see 7 1 Cleaning the installation on page 51 Take the aerofoil out the front slit and take the aerofoil outside the cabinet Take care whether you can take out the aerofoil does the cabinet have to be disinfected first See Appendix II Disinfection on page 67 Clean the sides and below side of the aerofoil see 7 1 Cleaning the installation on page 51 Clean all visible parts which are normally covered by the aerofoil see 7 1 Cleaning the installation on page 51 Place the aerofoil back Cleaning the worktop BIOLOGI CAL DANGER Never take the worktop out of an used installation without precaution for instance disinfection Clean the top side of the worktop see 7 1 Cleaning the installation on page 51 Stick the auxiliary key in the slots at the front side of the worktop Figure 26 Auxiliary key Fold the worktop up against the back parti
26. B Biological Safety Cabinet 7 Cleaning CYTOTOXI C DANGER When cytotoxics are used see 2 5 Cytotoxic danger EF B only on page 10 Only the responsible safety officer biological safety officer or the responsible examiner can decide if this procedure is valid or not Additional standard operation procedures can be necessary 7 1 Cleaning the installation The installation has to be cleaned regularly Preferably each time after the installation has been used Diluted disinfectants may be used In most cases hot water with a cleaning agent will be sufficient Chlorine containing cleaning agent is not recommended because even small concentrations chlorine may result in an oxidizing effect on the material of the installation It is important to clean the worktop see 4 6 Worktop on page 15 the space under the worktop and drip trays regularly A 1 solution of Natriumdodecylsulfat SDS solution CAS no 151 21 3 in demineralised water could be applied for cleaning Remove the waste and place it in an appropriate container Literature See Mallinckrodt J T Baker data sheet SDS is MSDS number No S3670 Mallinckrodt Baker Inc 222 Red School Lane Phillipsburg NJ 08865 Procedure for cleaning the cabinet BI OLOGI CAL DANGER Never clean a used installation without precaution for instance disinfection Switch the installation in the working mode see 5 4 Starting up the cabinet on page 2
27. Colour White Nr 840 White Nr 840 White Nr 840 White Nr 840 White Nr 840 Diameter mm 26 26 26 26 926 Length mm 970 1200 1500 970 1500 Power W 2x 36 2x 36 2x 58 2x 36 2x 58 Intensity mean 5 Lux 1000 1100 1550 1000 1550 EF 4 EF 5 EF 6 EF B4 EF B 6 Fuses O Board l F1 PCB 24 V A T 0 5 0 5 0 5 0 5 0 5 F2 Option A T 3 15 3 15 3 15 3 15 3 15 F3 Lighting A T 1 6 1 6 1 6 1 6 1 6 F4 UV A T 0 5 0 5 0 5 0 5 0 5 F6 Socket 1 A T 3 15 3 15 3 15 3 15 3 15 F7 Fan A T 8 8 8 8 8 F8 PCB A T 1 6 1 6 1 6 1 6 1 6 F9 PCB A T 1 6 1 6 1 6 1 6 1 6 l EF 4 EF 5 EF 6 EF B4 EF B6 Fuses option board F1 Optional fan A T Max 8 Max 8 Max 8 Max 8 Max 8 F2 Optional A T Max 8 Max 8 Max 8 Max 8 Max 8 F3 Charge current A T 1 1 1 1 1 EF 4 EF 5 EF 6 EF B4 EF B6 Control 4 Software version I O 35 T x5 35 Board Software version 4 9 4 9 4 9 4 9 4 9 Interface Options EF 4 EF 5 EF 6 EF B4 EF B6 UV Type UV C UV C UV C UV C UV C Power W 30 30 30 30 30 Length UV wave nm 253 253 253 253 253 Diameter mm 28 28 28 28 28 Length mm 908 8 908 8 908 8 908 8 908 8 EF 4 EF5 EF 6 EF B4 EF B6 Gas valve Voltage a 24 24 24 24 24 Current A 0 4 0 4 0 4 0 4 0 4 EF 4 EF 5 EF 6 EF B4 EF B6 Bunsen burner d Voltage V g DC 9 9 9 9 9 Power W 2 2 2 2 2 See also Appendix XI Required specifications on page 89 and Appendix XII Part lis
28. EPA filter The HEPA filter needs to be replaced when the HEPA filter integrity is not within specifications and or when it is not possible to adjust the air velocity according the specifications For the downflow HEPA filter the following applies e The downflow velocity of the cabinet must have an average air velocity of 0 36 0 02 m s measured at 10 cm above the bottom edge of the sliding window in work position None of UM EFAB EN10 doc 68 No M a Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet the measured values should deviate more than 2096 from the average value See Appendix V Measuring method air velocities on page 76 for more specifications e In case HEPA filter Integrity is measured with a photometer and DOP EMERY 3004 or DEHS as test aerosol then the maximum local penetration should not exceed 0 0196 If the required Integrity cannot be met then it is allowed to repair up to 5 of the surface area of the HEPA filter Alternative method In case HEPA filter Integrity is measured with a particle counter then local penetration should be lt 0 05 When the overall Integrity is measured the penetration should not exceed 0 005 Yo Exhaust HEPA filter The HEPA filter needs to be replaced when the HEPA filter integrity is not within specifications and or when it is not possible to adjust the air velocity according the specifications For the exhaust HEPA filter the following applies e The aver
29. F Astrocell A99C9S2R3L 15P6 610X1524X69 mm H14 acc EN 1822 Exhaust HEPA filter EF 5 1000606 AAF Astrocell A99C952R3 6P6 610X610X69 mm H14 acc EN 1822 Pre filter EF 5 1050001 G3 EN 779 Torsion spring EF 5 2700675 Coldwell 400mm 26 kg Fan EF 5 3000002 Ziehl Abegg RD25S 4EW 4N 1L 1 piece 230 V 10 50 Hz Pi 0 93 kW 4 0 A 1150 rpm 12 pF 400 V 50 C 18 kg Gas spring EF 5 2702008 ACP 150mm 300N FL lighting EF 5 1440019 Osram Lumilux L36W 21 Ballast EF 5 1240002 Osram VSA 40 W Optima Downflow HEPA filter EF B 6 1001816 AAF Astrocell A99C9S2R3L HEPA 18P6 610x1830x69 mm H14 acc EN 1822 Exhaust HEPA filter EF B 6 1000607 AAF Astrocell A99C952R3 HEPA 7P6 762x610x69 mm H14 acc EN 1822 First HEPA filter EF B 6 1008027 SPX HEPA V pleats Type PB H14 Nr 1154 0110 H14 acc EN 1822 12 pieces Pre filter EF B 6 1050001 G3 EN 779 Torsion spring EF B 6 2700676 Coldwell 400mm 32kg Fan EF 6 3000002 Ziehl Abegg RD25S 4EW 4N 1L 1 piece 230 V 10 50 Hz Pi 0 93 kW 4 0 A 1150 rpm 12 pF 400 V 50 C 18 kg EF B 6 3000002 Ziehl Abegg RD25S 4EW 4N 1L 2 pieces 230 V 10 50 Hz Pi 0 93 kW 4 0 A 1150 rpm 12 pF 400 V 50 C 18 kg Gas spring EF B 6 2702003 ACP 150mm 325N FL lighting EF B 6 1440017 Osram Lumilux L58W 21 Ballast EF B 6 1240001 Osram VSA 40 W Optima UM EFAB EN10 doc 91 EuroFlow EF and EF B Biological Safety Cabinet e M Clean el
30. OW usu sdubpueu poq ssn SABMIY Speo uorpnisuoo MOpUI ay VIGO AOO IAMS ap vado pue AAP y ii umop Aem au je opui ayi apiis uonisod Bunpaj ui Suid 6uppoy LM uedo sejjoud apis ayi ebur e oge poou do ayi vado o PEW J PAWAS Si LONENEISU JUL uomnq 3AvSu3MOd PUI usnd mopuim Buipiis ap sp OM Ye dogs asn ui si auiypew au amu pueau eq ue jeufrs IASNOJE eu pue dn SyS JOJEDIPUI Wa pay au y peayuelen6 aq Jabuaj ou ue suonipuoo GUDOM ayes YO ob quw jeubr DASNODE au pue JAJEAPW WY TW SUL YO peus si Guay6y ayi YO PEURS SI JOJYINUSA SUL W Usnd 3P0D sasn ay Jau Ae dsip eu uo pejsenbai si apoo Ssn ayi uognq oun usnd Do SUSIDAq Pen S1 O1 SDUEDUGDOE UI ISUUEUI aiqsuodsa e ui sevayew y0 pue spinbi ids je moway dn s46y JOJEIIPU 3GOW NYOM Useub ayi ip ypeau useq ney suonipuoo ne paWnbeu ay se uoos se days pim syeubks yog eseyd dn Gurney u ajqissod Auo AJmews ud jeubis 2ysnove ayi pou A VONNG v IR usnd 0 srqsssod si 3r dn SIUI JOJEAPW WAVTY pe ayi uo peus s uej ayi 1000 SI apoo puepuejs ayi YONN v SY uy epoo si uuo epoo SSN ap UF UONG Ued eu usnd A MOTANI 930 MOT1JNMD pesso 1snvux3 Figure 50 Short manual EF B series 20P TONS WN SHOMSA XX V43 voneuoju ansuape Joj jenuew sn 22S 43 edAj mojyoung euas sousrayan JAND poou da ay a500 uogisad Gupuom ozu mopu ayi apris Peq yad epis y Guy pue SPI aus ay SA pu
31. Push button to turn off the cabinet The display will ask for the user 2 code only when the user code is activated The fan will be switched off e Enter the user code and push button A Cabinet switched off Not Safe 3 Monday 11 06 33 MENU Socket Of f 5 8 Control UV optional The UV function is only visible if this option is installed and with a closed window In all other cases the UV will be switched off and the function at the display is invisible UV can only be switched on with a closed window This prevents direct contact on the skin or eyes of the personnel Switching UV on The window must be closed on the aerofoil or in disinfection mode Cabinet must be switched off or in power safe mode 1 The display shows UV Off UM EFAB EN10 doc 29 No M Clean A Telstar EuroFlow EF and EF B Biological Safety Cabinet s The UV will switch on Push EB uv orr The display shows UV On e Pl Dutton UVO Cabinet switched off Not Safe 2 Microbiological Safety Cabinet UV switched on MENU Socket Of f Switching UV off The UV will switch off gn The display shows UV Off e Push button UV On Cabinet switched off Not Safe Microbiological Safety Cabinet UV switched off MENU Socket Of f If the window is opened during a working UV light the UV will stop FL Fluoresced lamp
32. Rn l AN ie Figure 43 Measuring points downflow W Width D Depth UM EFAB EN10 doc 77 eee EuroFlow EF amp EF B Biological Safety Cabinet Clean Air Techniek B V Clean Ar Appendix VI Lay out boards Interface board Ji Flat cable I O print Figure 44 Interface board Control board Fa 3 pp eg Cr EBEN n 2 1 eE EBRO emis n ja Ji 15 J n 19 m J25 rm 3 Li p pr ed F6 JI INTERFALE BOARD FI 0 SAmp T J2 RS232 288 J10 0PT POWER F2 3 15Anp T J3 BACK UP BATTERY 24 JI6 FAN2 F3 1SAmp T JA BUNSEN BURNER 3 V 0 4 A J7 FAN CAPACITOR 2 FG 6 5Amp T JS GAS VALVE 241 0 414l JAB FAN 1 J6 ALARM 48 VIA1 A JIS FAN CAPACITOR 1 F6 3 15Anp JT QPTIQNAL INPUT J20 LIGHT 250 V 15 A F BAnpsT Jat uv 250 v 0 5 A FB 16Amp T JS FLOWSENSORS J22 BOOSTER FAN 250 1 3 1514l F9 16Amp T J23 SQCKET1 i250 v 3 15 A Jit WINDD W DETECTION A24 MAIN SUPPLY 250 Y J12 RS485 0B J25 5UPPLY 08 250 Y 2 A J30 ISP Figure 45 Control board EO10001 rev F UM EFAB EN10 doc 78 M M A Clean A Telstar EuroFlow EF and EF B Biological Safety Cabinet Appendix VII Alarm test procedure Authorized personnel only To test the alarm limits the automatic fan control must be overruled so the fan power can be set by hand The entire state of the cabinet will stay unchanged except for the fan power The required test code is 7733 In this state each 30 second two sho
33. T T security J6 Alarm potential free V Max 48 Max 48 Max 48 Max 48 Max 48 contact A Max 1 Max 1 Max 1 Max 1 Max 1 Electrical insulation Class 1 Class 1 Class 1 Class 1 Class 1 Electrical safety IEC EM assi disa A ass ass ass ass ass 61000 4 1 1 II II II II II Installation cat Power sockets Uma V 230 230 230 230 230 Imax A 3 15 3 15 3 15 3 15 3 15 Pmax W 725 725 725 725 725 Power consumption in standby mode with D ne 303 513 568 533 766 light switched off excl ta 13 2 2 2 5 2 3 3 3 maximum power socket Power consumption in work mode with light un 552 709 877 805 950 switched on excl Ion A 2 i 2 id k maximum power socket Power consumption in work mode with light gon 1277 1434 1600 1530 1675 switched on incl Thom A n a ii d maximum power socket Power consumption in max mode fan with pula 725 992 1036 1613 1956 light switched on excl 1 A ue 8 in l n maximum power socket Power consumption in max mode fan with Devi 1450 1717 1761 2338 2681 light switched on incl 1 A a 2 Ld aa d maximum power socket UM EFAB EN10 doc 63 EuroFlow EF amp EF B Biological Safety Cabinet Clean Air Techniek B V Clean Ai TORS EF4 EF 5 EF 6 EF B 4 EF B6 Lighting 4 TL High output 2x 2x 2x 2x 2x
34. age velocity of the air inflow should be gt 0 50 m s The air velocity measured perpendicular to the air flow at the top side of the work aperture should be 2 0 28 m s e In case HEPA filter Integrity is measured with a photometer and DOP EMERY 3004 or DEHS as test aerosol then the maximum local penetration should not exceed 0 0196 If the required Integrity cannot be met then it is allowed to repair up to 5 of the surface area of the HEPA filter Alternative method In case HEPA filter Integrity is measured with a particle counter then local penetration should be lt 0 05 When the overall Integrity is measured the penetration should not exceed 0 005 Yo The first V shaped HEPA filters EF B The HEPA filter needs to be replaced when the HEPA filter integrity is not within specifications and or when it is not possible to adjust the air velocity according the specifications For the first V shaped HEPA filters only relevant to the EF B class II cabinets the following applies e The downflow velocity of the cabinet must have an average air velocity of 0 36 0 02 m s measured at 10 cm above the bottom edge of the sliding window in work position None of the measured values should deviate more than 2096 from the average value See Appendix V Measuring method air velocities on page 76 for more specifications e The average velocity of the air inflow should be gt 0 50 m s The air velocity measured perpendicular to the air flo
35. ating microprocessor regulates the fan speed to compensate for main power fluctuations and filter pollution o Independent potential free output contact interface board connector J6 See Interface board on page 78 Main filter Light ballast starter for UV Option Light ballast for FL light Option board see Appendix VI Lay out boards on page 78 Oo O 0 0 UM EFAB EN10 doc 17 e NI f Clean Ai Telstar EuroFlow EF and EF B Biological Safety Cabinet 4 19 Operating panel Clean Air J EB Figure 7 Operating panel The operating panel Figure 3 Figure 4 Figure 5 and Figure 6 Pos 19 and Figure 7 consists of 1 Display 2 Function keys 4x 3 Fixed keys 4x 4 Signals 4 20 FL lighting The Fluorescent lighting Figure 3 Figure 4 Figure 5 and Figure 6 Pos 20 is assembled behind the top hood 4 21 Fan The fans with the motor Figure 3 Figure 4 Figure 5 and Figure 6 Pos 21 are maintenance free double suction fans The Air Velocity Compensating Microprocessor controls the fan power for any disrupting factors such as voltage drops or filter pollution With an installation with a double exhaust HEPA filter an additional fan is built in The fan regulations are mounted behind the light hood 4 22 Optional Fittings Figure 3 Figure 4 Figure 5 and Figure 6 Pos 22 Gas connection This connection is assembled together with a gas valve Normally both the inlet and outlet gas c
36. ce inform you service organization Change the user code See Appendix X User code on page 87 UM EFAB EN10 doc 45 EuroFlow EF and EF B Biological Safety Cabinet Change language Clean A Merostar To change the language first close the window switch off the cabinet and all functions Then the language can be changed and confirmed As confirmation the 3 LED s right light up for 1 second and 2 short beeps will sound The cabinet will be switched on automatically Cabinet in work mode See 5 4 Starting up the cabinet 1 on page 26 Change the language Cabinet in work mode Safe Monday 11 06 33 MENU Socket Of f e Push button En Menu e Push button EB o e Push 5x button HEB gt e Push button i Conditions The fan has to be switched off push d the window has to be closed completely and sockets and FL light must be switched off Language Sprache English Englisch Change e Push button NE Change to change the language e Push button UM EFAB EN10 doc 46 EuroFlow EF amp EF B Biological Safety Cabinet Clean Air Techniek B V _ Clean An ac Air Clean Air Techniek B V Figure 18 Operating panel When there is an alarm situation the alarm indicator flashes red and there is an acoustic signal The safe working conditions are no longer granted Push button for giving an acknowledgement that the user has seen the alarm The a
37. de fan ON OFF A Acknowledge Enter Function keys The function of keys 1 2 3 and 4 is variable In the last line of the display the function is shown Signals Alarm Confirmed alarm state Alarm flashing along with acoustic signal Actual alarm LUV Indication UV light switched on TA OK Safe user mode UM EFAB EN10 doc 25 Ne Clean Ar Telstar EuroFlow EF and EF B Biological Safety Cabinet s All equipment and if possible all materials required for working with a safety cabinet need to be placed into the installation before starting up the cabinet Equipment or objects with a height up to 350 mm can be placed in the working space by sliding the window upwards In case of larger objects the window can be hinged Be careful keep the safety regulations in consideration 5 4 Starting up the cabinet The lighting of the display will light up The alarm indicator light is red e Connect the power cable Cabinet switched off not safe Monday 11 06 33 MENU Socket Of f Use both handgrips when hinging or sliding the window e Put the window in the working position see marks on the installation W The fan starts Cabinet is in start up mode The alarm indicator light is red There is an acoustic alarm e Push button a Starting to work mode not safe Monday 11 06 33 MENU Socket zOff e To acknowledge this alarm pus
38. e downflow HEPA filter D and the exhaust HEPA filter F The extracted air of the cabinet can recirculate into the laboratory or can be blown into an exhaust channel In case the cabinet is used for cytostatics production the cabinet has to be connected to an external exhaust channel UM EFAB EN10 doc EXHAUST ie iS 5 A an ZO 18 Xo DOWNFLOW INFLOW i yi A a 8 Figure 2 Sectional view EF B No M Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet 2 Safety 2 1 Use in conformity with regulations Personnel that is not properly informed about the safety regulation and or does not meet the requirements mentioned in 2 3 Authorized persons is not allowed to use the cabinet Improper use of the cabinet is not allowed 2 2 Modifications and changes to the installation In order to operate the installation safely changes and or alterations shall only be made by Clean Air Techniek B V or shall be made after consultation with and permission in writing from Clean Air Techniek If modifications and changes are made on own initiative without permission from Clean Air Techniek B V the parts of the installation concerned are no longer covered by the warranty Clean Air Techniek B V cannot be held liable for the consequences of the modifications and the dangers which might possibly occur as a result The modificat
39. e sud Buppo aui AOUN opui ayi umow usum sdubpueu oq asn SABMIY koni e MOpUIM n an sps1ewoj uonprnsuoo HOpUVM an vedo DPA OAS ayi Lado pue SPIP y IN UMOP ABA ayi je opui ayi 9pirs vonisod Burpa ui Suid buppor uun uado sejyoud apis ayi S6UH 9 oge poou doy an vedo pjeau eq ue jeulis nsnooe y pue dn Sut JOgeOlput Wavy Pou eu y pesyuesen6 aq Jabuaj ou ue SUQYPUOI GUDOM ayes YO OB qw eub 2gsnooe AY pue JOJEAPU WAY TW SUL YO PAAMS si Guay6l ayi YO peuos si JOELA ayi v usnd 3p00 asn ay Jeu ejdsip eu uo pexsenbai s apoo s iesn ayi young JOLVIINBA 9 usng dn SYON Jo3eoipur GOW NYOM useub ayi payeau useq eeu suonipuoo are paWnbeu y se uoos se days lm sjeu amp rs u3og aseyd dn Buruess ur ejqissod Auo Ajeumeusud jeubis asno ayi pue a VOTING W eu usnd o1 ajqrssod si IT dn SIGI 10e5iput WIV TY pex ayi uo PINS si UE ayi 1000 SI 9poo puepuejs ayi uonnq v BY uy epoo si uuguo epoo s jasn etg JUF vannq Ued au usng Figure 49 Short user manual EF series 93 UM EFAB EN10 doc EuroFlow EF and EF B Biological Safety Cabinet cm Sretstar Appendix XI V TUV Low Pressure Mercury Lamps Special fluorescent lamps Philips TUV disinfection lamps are low pressure mercury vapour discharge lamps consisting of a tubular glass envelope emitting short wave ultraviolet radiation with a radiation peak at 253 7 nm UV C for germicidal action The glass filters out
40. e test Yes Type test Yes Type test Yes Type test Yes Type test Yes Installation test Yes Type test Yes Type test Yes Installation test Yes Type test Yes An abstract of the Type test report can be sent on request by Clean Air Techniek B V UM EFAB EN10 doc 90 EuroFlow EF and EF B Biological Safety Cabinet e M Clean elstar Appendix XI I Part list critical components Component Type Art no Manufacturer Type specific Downflow HEPA filter EF B 4 1001216 AAF Astrocell A99C9S2R3L HEPA 12P6 610x1220x69 mm H14 acc EN 1822 Exhaust HEPA filter EF4 1000404 AAF Astrocell A99C952R3 HEPA 4P4 457X457X69 mm H14 acc EN 1822 Exhaust HEPA filter EF B 4 1001406 AAF Astrocell A99C952R3 HEPA 4P6 457x610x69 mm H14 acc EN 1822 First HEPA filter EF B 4 1008027 SPX HEPA V pleats Type PB H14 Nr 1154 0110 H14 acc EN 1822 8 pieces Pre filter EF B 4 1050001 G3 EN 779 Torsion spring EF B 4 2700674 Coldwell 550mm 22 kg Fan EF 4 3000033 EBM D4E 225 CC 01 230V 50Hz 650W 2 84 A 1150 mint 25 UF EF B 4 3000030 EBM Ziehl Abegg RD28S 4EW 4R AL 230 V 1096 50 Hz Pi 1 5 kW 6 7 A 1150 rpm 25 pF 400 V 40 C 26 kg Gas spring EF B 4 2702006 ACP 150mm 250N FL lighting EF B 4 1440016 Osram Lumilux L36W 21 01 Ballast EF B 4 1240002 Osram VSA 40 W Optima Downflow HEPA filter EF 5 1001516 AA
41. e uniformly corrosion resistant non flammable and cleanable Materials and sealants for joints should be durable and resistant to cleaning and disinfection agents and resistant to general use of MSCs Manufacturers should ensure that the materials of construction will not be damaged by fumigation with formaldehyde Laminated safety glass or UV resistant safety plastics Conform to EN 61010 1 Maintenance can be carried out safely on the cabinet after installation Considered according to prEN ISO 14738 1997 Air temperature in work space 8 above ambient laboratory temperature No visible leakage with soap bubble test Microbiological test method or potassium iodide KT method Particle counter lt 0 05 locally counted Aerosol photometer 0 0196 300 CFU of B subtilis DIN 12980 Chapter 11 DIN 12980 Chapter 12 DIN 12980 Chapter 12 DIN 12980 Chapter 7 DIN 12980 Chapter 6 DIN 12980 Chapter 6 DIN 12980 Chapter 6 DIN 12980 Chapter 6 DIN 12980 Chapter 11 DIN 12980 Chapter 9 DIN 12980 Chapter 7 DIN 12980 Chapter 7 DIN 12980 Chapter 7 EN 12469 Annex B DIN 12980 Chapter 16 EN 12469 Annex C2 C3 EN 12469 Annex D DIN 12980 Chapter 17 EN 12469 Annex E DIN 12980 Chapter 17 EN 12469 Annex F DIN 12980 Chapter 15 or EN 12469 Annex G EN 12469 Annex H Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Type test Yes Typ
42. echniek B V Version table Ver 10 0 02 08 10 MPE Logo updated preface revised Version Date From serial By Check Change number Copyright 2010 All rights reserved Nothing from this edition may be copied or used in an automated database or made public either electronic mechanical by photocopies recordings or in any manner without preceding written permission of Clean Air Techniek B V This also counts for the accompanying figures and or plans Clean Air Techniek B V is entitled to change parts on each desired moment without preceding or direct notification of the customer The content of this user manual can be changed without preceding warning as well For information concerning maintenance activities or repairs which are not mentioned in the user manual please contact the service organization This user manual has been put together with all possible care but Clean Air Techniek B V cannot take the responsibility for possible mistakes in this user manual or for the consequences of it UM EFAB EN10 doc 2 ee Ne m a Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet TOC 1 Introduction 6 1 1 EF 6 1 2 EF B 7 2 Safety A OO 11 1 1 1 11 111 1 1 LL 8 2 1 Use in conformity with regulations 8 2 2 Modifications and changes to the installation 8 2 3 Authorized p
43. ed the safe working area for information about the dimensions see 9 2 General specifications on page 61 At the front and the back there are air slits which are essential to the functioning of the installation At the two sides two panels have been placed See detail Pos 6 4 7 Aerofoil The aerofoil Figure 3 Figure 4 Figure 5and Figure 6 Pos 7 is used for a better air conduction and to support the elbow During work with the cabinet the aerofoil needs to be installed The aerofoil is made of coated aluminium for better ergonomics less weight and less heat transportation 4 8 Plenum construction The plenum construction Figure 3 Figure 4 Figure 5and Figure 6 Pos 8 divides the air which is let in by the fan between the downflow HEPA filter and the exhaust HEPA filter UM EFAB EN10 doc 15 Clean Wekt EuroFlow EF and EF B Biological Safety Cabinet 4 9 Drip tray pre filter only EF B and as an option for the EF The pre filter with drip tray EF Figure 3 and EF B Figure 5 Pos 9 is assembled under the worktop The installation is equipped with this to protect the interior of the installation against pollution by coarse materials and to collect spilled fluids Furthermore the pre filter will enhance the life span of the HEPA filter significantly 4 10 Downflow HEPA filter The downflow HEPA filter Figure 3 Figure 4 Figure 5 and Figure 6 Pos 10 has a very high efficiency Class H14 in accordance with EN1822 Aft
44. elf is no disinfection The Biological Safety Cabinet can work according to automatic disinfection software only if this has been installed Consult your order confirmation for the installed options or contact Clean Air Techniek B V In case additional information about disinfection is needed please contact Clean Air Techniek B V Check the website of Clean Air Techniek B V www cleanair eu Products Downloads for more detailed information about disinfection UM EFAB EN10 doc 67 M M a Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet Appendix lll Replacement of the HEPA filters Under normal circumstances the life span of a HEPA filter comprises several years However it will become clogged up gradually until there is no longer a proper flow through and the filter has to be replaced Replace both the downflow filter and the exhaust filter at the same time This needs to be done since the total amount of air in the cabinet is based on filter proportion In cabinets of the EF B series the first HEPA filters will be clogged up faster than the downstream positioned downflow and exhaust HEPA filters BI OLOGI CAL DANGER BE CAREFUL Before starting service with the cabinet a written declaration that the cabinet is decontaminated must be present The responsible person laboratory supervisor will decide if the cabinet has to be decontaminated so that when servicing the installation and the HEPA filters the
45. er assembling each filter will be tested separately according to EN12469 2000 Annex D Through the service panel the downflow filter can be reached Pos 15 4 11 Downflow protection The downflow filter has a build in protection grid Figure 3 Figure 4 Figure 5 and Figure 6 Pos 11 4 12 Exhaust HEPA filter The exhaust HEPA filter Figure 3 Figure 4 Figure 5 and Figure 6 Pos 12 has a very high efficiency Class H14 in accordance with EN1822 After assembling each filter will be tested separately according to EN 12469 2000 Annex D Through the topside of the cabinet the exhaust filter can be reached 4 13 Support frame optional For a stable positioning of the cabinet we strongly recommend the use of the especially designed support frame from the supplier Figure 3 Figure 4 Figure 5 and Figure 6 Pos 13 This support frame includes an in steps adjustable feet support for a good ergonomic work position Figure 3 Figure 4 Figure 5 and Figure 6 Pos 14 As well the feet support will increase the stability of the cabinet In addition it is recommended to use an in height adjustable well disinfectable chair to optimize the work position 4 14 Feet support assembled on 4 13 The support frame includes an in steps adjustable feet support for a good ergonomic work position Figure 3 Figure 4 Figure 5 and Figure 6 Pos 14 As well the feet support will increase the stability of the cabinet In addition it is recomme
46. ersons 8 2 4 General safety aspects 9 2 5 Cytotoxic danger EF B only 10 2 6 Explanation user safety symbols 10 3 Installation _ 11 3 1 Assembly instruction 11 3 2 Transport instructions 12 3 3 Dismantling amp Disposal 12 4 Product description 13 4 1 Type plate 14 4 2 Short user manual sticker 14 4 3 Body of the cabinet 14 4 4 Hinge slide window 15 4 5 Top hood 15 4 6 Worktop 15 4 7 Aerofoil 15 4 8 Plenum construction 15 4 9 Drip tray pre filter only EF B and as an option for the EF 16 4 10 Downflow HEPA filter 16 4 11 Downflow protection 16 4 12 Exhaust HEPA filter 16 4 13 Support frame optional 16 4 14 Feet support assembled on 4 13 16 4 15 Front panel 16 4 16 Back panel 17 4 17 Main power connection 17 4 18 Electronics 17 4 19 Operating panel 18 4 20 FL lighting 18 4 21 Fan 18 4 22 Optional Fittings 18 4 23 Sockets 21 4 24 Housing of the first HEPA filter EF B only 21 4 25 First HEPA filters EF B only 21 UM EFAB EN10 doc 3 Clean No EuroFlow EF and EF B Biological Safety Cabinet Telstar 5 Control A LLL LLL 22 5 1 Using the cabinet 22 5 2 Top hood sliding window 22 5 3 Display interface 25 5 4 Starting up the cabinet 26 5 5 Locking functions cabinet 27 5 6 Power save mode 28 5 7 Turn off 28 5 8 Control UV optional 29 5 9 Control gas supply optional 30 5 10 Contr
47. filter and exhaust filter Clean Air Techniek bv Kuipersweg 37 NL 3449 JA Woerden The Netherlands e t 31 0 348 411114 gn f 422 31 0 348 422684 einfoQcleanairbv nl E Air s i www cleanairbv com Filter type Astrocel 2 Hepa 12P4 Serial number 7459707 2 Date 31 07 2007 3 Sticker I O board CONNECTORS Interface board F J25 424 i a RS 232 285 Ja a Ao Back up battery 24 V Bunsen burner 9 V 0 25 A no 0 0 ti i t Gas valve 24 V 0 4 A Alarm 48 V 1 A W Optional input lr Eee 230V Optional power Window detection RS 485 OB H Fan2 L Fan capacitor 2 Fan 1 C Fan capacitor 1 ame Lo Light 250 V 1 5 A JEJE JE ee ca ane Booster 250 V 3 15 A Socket 1 250 VI 3 15 A FUSES F3 Light 1 5 A T 250 V F7 Fan 8 A T 250 V Main supply 250 V F1 PCB 0 5 AJT 250 V F4 UV O S AJT 250 V F8 PCB 1 6 A T 250 V Supply OB 250 V 2 A F2 Option 3 15 A T 250 V F6 Socket 1 3 15 A 250 V F9 PCB 1 6 A T 250 V ISP UM EFAB EN10 doc 81 M cl EuroFlow EF and EF B Biological Safety Cabinet elstar 4 TUV sticker geprifte Sicherheit 5 Work mode Workposition calibrated 6 Electro 7 Biohazard Biohazard sticker is positioned on the cabinet The sticker has to positioned in sight of the operator 8 Cytotoxic only EF B This sticker is delivered with the cabinet Depending on the work that is done on the cabinet the customer m
48. ft hand as long as this is done consistently this procedure needs consistence due to contamination danger UM EFAB EN10 doc 56 e No Clean A Telstar EuroFlow EF and EF B Biological Safety Cabinet Supplies needed Usermanual Plastic bag minimum measurement 75x50 cm with closure Two pairs of gloves Tape Working surface hook auxiliary hook Possible disposing requirements New pre filter measurements of the pre filter are necessary when ordering a new one the measurements are printed on the type plate see 4 7 Type plate on page 14 and Appendix VIII Stickers on page 81 Figure 28 Supplies needed Procedure Switch on the installation Make sure that the window is in the working position and that there are no alarms Put on the gloves and keep an extra pair ready for use on the aerofoil Fold the edge of the plastic bag 10 cm tro cm Figure 29 Folding the edge of the bag 10 cm Lift up the worktop with your left hand using the working surface hook and put it against the back partition with your right hand UM EFAB EN10 doc 57 e NI Clean A Telstar EuroFlow EF and EF B Biological Safety Cabinet Figure 30 Lifting up the worktop against the back partition Place the opening of the plastic bag inside the front aperture with your left hand Figure 31 Positioning the left hand in the plastic bag Figure 32 Positioning the plastic bag on the aerofoil Positioning
49. ghting switches off The UV light can only be switched on when the window is closed window on aerofoil or when the cabinet is in disinfection mode aerofoil lifted up and window at its lowest point this makes sure the user will not get in contact with UV C radiation The UV switch function EB is only visible when the window is closed DANGER FOR BURNI NG Avoid direct contact with UV C radiation This causes burns to the skin and eyes DANGER UV C RADIATION PROTECT EYES AND SKIN The UV light can be switched on and off on the display see 5 8 Control UV optional on page 29 It is also possible to adjust the UV disinfection for each day a certain disinfection period can be defined see 55 11 Set up 7 days clock UV on page 31 Do not touch the UV lamp with the hand and keep the lamp fat free otherwise the lifetime will decrease The Watch Out UV light must be cleaned with alcohol 7096 regularly switch off the installation See 7 2 Cleaning the UV light optional on page 53 UM EFAB EN10 doc 20 Clean Wekt EuroFlow EF and EF B Biological Safety Cabinet When the UV is switched on the UV hour counter counts the lighting period UV lamps have depending on fabricate and type a limit in effective running hours Replace the lamp frequently Alternative Options Please contact Clean Air Techniek B V for information about alternative options 4 23 Sockets The sockets are a
50. h button A If the cabinet is secured with a user code this code has to be entered first The default code is 0001 The user code can be changed by the user see Appendix X User code on page 87 e Enter actual user code User code 0000 Enter code e Confirm the code with button A The light will switch on e Push button ED When the fan has reached the right airspeed the alarm indicator will disappear The following display will be shown Cabinet in work mode safe Monday 11 06 33 MENU Socket Of f However the cabinet must operate for minimally 15 minutes more before the cabinet is ready to work with UM EFAB EN10 doc 26 EuroFlow EF and EF B Biological Safety Cabinet If the air speeds still are not right the alarm will sound again To see what is wrong See 5 13 Information status cabinet on page 40for more info about this menu Cm Sreistar safe Starting to work mode not Monday 11 06 33 MENU Socket Of f e Push button EN Menu A gt Hour counter information B gt Status cabinet SS Sere tele A e Push button A Cabi net Downf low Working pos Window Working pos 0 29 0 36 0 43 ms 0 24 x 0 972 0 35 as Inflow lt 1 e Back to starting menu button En Esc ai The average inflow velocity at the top of the front aperture is measured and can be read in the disp
51. ilter 10 Downflow filter 11 Downflow protection build in 12 Exhaust filter 13 Support frame optional 14 Feet support 15 Front panel 16 Back panel 17 Mains connection 18 Electronics 19 Operating panel 20 Lightning 21 Fan 22 Optional fittings 23 Sockets EF B only 24 Housing of first HEPA filter 25 First HEPA filter OONOU 30 PN m 41 Type plate The type plate Figure 3 Figure 4 Figure 5 and Figure 6 Pos 1 mentions instructions that are important for the connection of the biological safety cabinet See Appendix VIII Stickers on page 8l 4 2 Short user manual sticker The short user manual Figure 3 Figure 4 Figure 5and Figure 6 Pos 2 gives a brief overview of the primary functions and is included with the cabinet See also Appendix XIII Short user manual on page 93 4 3 Body of the cabinet The body of the installation Figure 3 Figure 4 Figure 5and Figure 6 Pos 3 is made of epoxy coated steel The interior of the body is made of stainless steel 304 The body is leak tight as per LI C table 1 and 4 of the EN 12469 2000 standard The body has been tested according to the method described in annex 2 of the EN 12469 2000 UM EFAB EN10 doc 14 No M a Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet 4 4 Hinge slide window The hinge slide window Figure 3 Figure 4 Figure 5 and Figure 6 Pos 4 is a tightly closing manually operated glass window It can be slid
52. ine 58957 92 9 52 9 Mixantron Mitoxantrone 65271 80 9 Vinblastine 865 21 4 Vincristine 57 22 7 Vinorelbine Navelbine 71486 22 1 Example 2 Cytotoxic CAS no Recommended cleaner UM EFAB EN10 doc 54 EuroFlow EF and EF B Biological Safety Cabinet e M Clean elstar Chloormethine 51 75 2 Mechlorethamine 0 1 N Natronloog Sodium Dactinomycine 50 76 0 Hydroxide CAS no 1310 73 2 Estramustine 2998 57 4 Example 3 Cytotoxic CAS no Recommended cleaner Mitomycine C 50 07 7 0 1 N HCL Hydrochloric Acid CAS no 7647 01 0 UM EFAB EN10 doc 55 EuroFlow EF amp EF B Biological Safety Cabinet Clean Air Techniek B V Clean Ai 8 Small maintenance and testing BI OLOGI CAL DANGER BE CAREFUL Before starting service with the cabinet a written declaration that the cabinet is decontaminated must be present The responsible person laboratory supervisor will decide if the cabinet has to be decontaminated so that when servicing the installation and the HEPA filters there is no danger for the service engineer labour personnel the laboratory and the surroundings of the cabinet If necessary disinfect the installation For more information Appendix II Disinfection on page 67 CYTOTOXI C DANGER When cytotoxics are used see 2 5 Cytotoxic danger EF B only on page 10 Only the responsible safety officer biological safety officer or the respo
53. ions is given example Installed Options 1 Socket present UV inst Gas present 2 flowsens Biohazard Yes 3a Screen I 3b Screen II 3c Screen III From here there are 3 possibilities examples UM EFAB EN10 doc 41 EuroFlow EF and EF B Biological Safety Cabinet Poen ac Sretstar 3a Options Screen I Starting up from Step 2 Installed Options 1 Socket present UV inst Gas present 2 flowsens Biohazard Yes Option Installed options display shows e Push button MEM Option Option Exhaust fan Relays C2 For explanation codes see Appendix IX Information installed options on page 84 e Push 2x button ZB Esc for starting menu 3b Options Screen II Starting up from Step 2 Installed Options 1 Socket present UV inst Gas present 2 flowsens Biohazard Yes Installed options display shows e Push button ME gt Installed Options Relays A1 Relays B1 For explanation codes see Appendix IX Information installed options on page 84 e Push button ENB Esc for starting menu Options Screen III Display only appears when analogue options are installed Pou e Push 2x button BEI gt Analog inputs Starting up from Step 2 AD 0 E AD2 1 0 pM 3c For explanation codes see Appendix IX Information installed options on page 64 Push button B Esc for starting menu 5 14 Settings In setti
54. ions mentioned here include the connection of the installation to an exhaust system 2 3 Authorized persons Operating the installation The cabinet shall only be used by personnel which e is familiar with the content regulations and warnings which are mentioned in this operating manual e is familiar with how to operate the cabinet e is familiar with the start up procedure the stop procedure and Knows how to react in case of emergency e is familiar with the regulations to keep the safety in all circumstances e is capable to end a disturbance Maintenance and repair general Maintenance shall only be performed by professional service engineers who are sufficiently trained by Clean Air Techniek B V to e Estimate and avoid the dangers of the installation e Estimate the consequences of their actions e Before starting maintenance to the installation mechanical and or electric disconnect the power supply Pay attention to rotating parts after switching off the installation the fans will still rotate for a while Maintenance and repair of the electrical installation Maintenance shall only be performed by professional electro technical service engineers who are sufficiently trained by Clean Air Techniek B V when e They can estimate and avoid the dangers of the installation e They can estimate the consequences of their actions UM EFAB EN10 doc 8 No M a Clean Telstar EuroFlow EF and EF B Biological Safet
55. ive makes it possible to lock the control of the installation To change this state the valid user code has to be entered The new state active or not active is valid after a period of 5 minutes after touching the last button The pre installed valid user code is 0001 In case of emergency there is a special code to break the unknown or forgotten user code In that case enter code 2882 and the original pre installed code 0001 is valid again Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Cabinet in work mode Safe Monday 11 06 33 MENU Socket Of f e Push button EB Menu e Push button Eo C e Push button HEB gt User code not active User code mode appears e Push button Function locked after 5 minutes 0000 Enter code User code Not active gt gt m The information for the user code shows The user code can be activated or deactivated e Enter the user code using button E gt gt and WEB e Push button User code Active e Push button SESS Esc for starting menu UM EFAB EN10 doc 87 EuroFlow EF and EF B Biological Safety Cabinet Changing the user code Cm X ferstar Cabinet in work mode See 5 4 Starting up the cabinet 1 on page 26 Change the user code Safe Cabinet in work mode Monday 11 06 33 MENU Socket Of f e Push button EB Menu and EB C e Push 4x button ME
56. l Safety Cabinet mon man e The day can be changed by pushing button EB or EB Change hours min sec e Push button EM gt gt again h i h hi 3 Setup display setup ie sdua e The ours min sec can be changed by pushing button time shows EB or EB Acknowledge the settings 4 ai e Acknowledge the setting with button A e Push 2x button ENB Esc for starting menu Actual time appears when there are no more alarms In case of power failure actual time is kept in memory for one month 5 13 Information status cabinet In this menu the settings of the cabinet can be checked to inform service personnel In information status more details are given about e Actual velocities alarm setting and fan settings e Information about the installed options Sockets UV e Information about Options relays e Information about Analog inputs only visible when option is activated Cabinet in work mode Cabinet in work mode Safe See 5 4 Starting up the cabinet Monday 11 06 33 on page 26 MENU Socket Of f e Push button EN Menu A gt Hour counter information 3 SS SES CELDT net C gt Settings A Status Display Status display shows Status Information e Push button p Downflow 0 36 Inflow 92 Cabinet Work mode Wi ndow Work pos 3 Example with colours Yuu au 0 29 0 36 0 43 Inflow s A W 32 0 38 gt c
57. l exhaust channel disconnect the cabinet from this exhaust channel Remove the nuts support plate of the exhaust HEPA filter Remove the support plate of the exhaust HEPA filter Remove the HEPA filter Put the HEPA filter in an appropriate bag Seal the plastic bag airtight Remove the gloves and put them in the appropriate container UM EFAB EN10 doc 71 EuroFlow EF amp EF B Biological Safety Cabinet Clean Air Techniek B V Clean Ai Position the new exhaust HEPA filter Unpack the new filter check specifications and register the serial number and specifications Filter certificate has to be stored on a safe place Put Vaseline on the seal of the HEPA filter both sides Position the HEPA filter take care for possible damage of the sealings Fasten the support frame In case the cabinet was connected to an external exhaust channel connect the cabinet to this exhaust channel Put off your gloves and put these in an appropriate bag Place the power plug in the supply socket and switch on the installation Perform an Integrity test according to the procedure described in Appendix IV Efficiency test of the HEPA filters on page 74 Replacement of the first V shaped HEPA filter EF B only For replacement of the first HEPA filter the following procedure must be followed Supplies needed e Plastic bag with closure minimum measurement depends on the volume of the HEPA filter e Gloves 4 pair for EF B 4 6 pai
58. lable SISISTZTZTSDTSRADIDE AIZ ZS Z S 8 S E E Sisjssg s g 2 8 i a eS els s s e elsa2sgSsS s eeESS i o sje t zi i 1 3 2 3 143 c lalo sol 22 POS S12 E ef Sg Li ct tlel i 28 S 3 i beaked bed FE of fo a7 mim m 2 a e w ki s n t ex ty fi X o hi uz ext eft az AH si gis ph jo RIS S 2 g 23232 ww 9 a ss a HEITA zis el A SIS Z Z E T L g amp g z EE z ziz 13 e ojs sss uo x Elbe i Zuli SSS ao o o 8 B 33 X 4 189i lt a a H ue xg x a H c EETA alsa HH dd A HE z iii al gaj jse efaji S sale sa 8 ol 3 5 oj Ejo l Slay 8i a e z S 2 s vz sf s 3x e sS iliS EIFIEIIPSEIEIIIE I P o o a IN 15 Mes 2j2jaj a 3 T s ss c r o o m la ked 8 3 8 Q Figure 35 Example of a support frame EF 6 UM EFAB EN10 doc 66 Clean Wiktar EuroFlow EF and EF B Biological Safety Cabinet Appendix Il Disinfection Your Biological Safety Cabinet has a built in disinfection software program This program knows a number of varieties which are dependent on the installed options in the security cabinet and the manner the cabinet has been installed The disinfection software of the control print is only available in version number 2 4 270307 or higher The disinfection software its
59. larm indicator will always keep on burning Before acknowledging the alarm the user code must be entered only when the user code is activated An acknowledged alarm with button A shall be repeated every 7 minutes as long as the alarm situation continues The buzzer has a SPL sound pressure level of at least 10 dB A more than the sound pressure of the working cabinet See Appendix XI Required specifications on page 89 In one band of the Terz band analysis mentioned in the technical reports from the TUV Nord GENERAL DANGER Make sure that there are no alarm situations during the use of the cabinet Never work with a cabinet with activated alarm signals The different types of alarm signals will be discussed in the following chapter 6 1 Most frequent window alarms Window is not in work position Window is not in work position Figure 19 Window above window position Person protection is less aerosols from the work area can go outside the cabinet because of a weakened air curtain Product protection is less aerosols from the outside can go into the cabinet because of a weakened air curtain UM EFAB EN10 doc 47 NI Pe A Clean A Telstar EuroFlow EF and EF B Biological Safety Cabinet Window below window position Person protection stays intact Product protection cannot be granted The inflow air through the work opening has a higher speed and may pass the front air slits
60. lay The minimal required value of the average inflow velocity measured at the top of the front aperture is given in chapter 9 2 on page 61 Measuring these most critical measured values directly under the window ensures that the overall average inflow velocity is according to the guidelines of the EN12469 2000 Annex H table H1 Mean inflow velocity to achieve operator protection 0 4 m s 5 5 Locking functions cabinet Only when the locking function is activated The cabinet will lock its functions after being 5 minutes in work 1 position In the status of being locked only a window alarm can be acknowledged When within these 5 minutes an arbitrary button is pushed e g lighting the timer starts counting again From this moment the fan buttons are not available anymore 2 The user code has to be entered to make the functions available again immediately after entering the Cabinet in work mode Safe Monday 11 06 33 Cabinet is locked with pincode MENU Socket Of f See Appendix X User code on page 87 If the cabinet has to be locked A 3 e Push button for 5 seconds After 3 seconds two short beeps will be heard as an UM EFAB EN10 doc 27 Clean A Sreistar EuroFlow EF and EF B Biological Safety Cabinet code acknowledgement the cabinet has been locked To unlock the cabinet the user See Appendix X User code on page 87 code has to be entered again
61. lter When this does not have sufficient effect the HEPA filter must be replaced as described in Appendix III Replacement of the HEPA filters on page 68 Test aerosol In an EF cabinet the test aerosol for the downflow and exhaust filter can be added through the working area Figure 36 Pos A The 100 measure point upstream concentration for the downflow filter as well as the exhaust filter is positioned under the work area Figure 36 Pos B and Figure 38 The downflow filter and the exhaust filter can be scanned Figure 36 Pos F and D In an EF B cabinet the test aerosol for the first HEPA filter can be added through the working area Figure 37 Pos A for each HEPA filter section separately UM EFAB EN10 doc 74 EuroFlow EF and EF B Biological Safety Cabinet Test aerosol for the downflow and exhaust filters can be added on the special connection on the Clean Ar reistar front of the first HEPA filter section Figure 39 The 100 measure point upstream concentration for the downflow filter as well as the exhaust filter is positioned under the work area Figure 37 Pos B and Figure 38 The downflow filter and the exhaust filter can be scanned Figure 37 Pos F and D Ty DOWNFLOW INFLOW EXHAUST teh d INFLOW Figure 38 100 measure point exhaust and downflow for EF and EF B exhaust HEPA fi
62. lters for EF B Figure 39 Input test aerosol for downflow and UM EFAB EN10 doc 75 Waste Clean EuroFlow EF and EF B Biological Safety Cabinet Appendix V Measuring method air velocities BI OLOGI CAL DANGER BE CAREFUL Before starting service with the cabinet a written declaration that the cabinet is decontaminated must be present The responsible person laboratory supervisor will decide if the cabinet has to be decontaminated so that when servicing the installation and the HEPA filters there is no danger for the service engineer labour personnel the laboratory and the surroundings of the cabinet If necessary disinfect the installation For more information Appendix II Disinfection on page 67 CYTOTOXI C DANGER When cytotoxics are used see 2 5 Cytotoxic danger EF B only on page 10 Only the responsible safety officer biological safety officer or the responsible examiner can decide if this procedure is valid or not Additional standard operation procedures can be necessary The measurement of air velocities in the cabinet is performed according to the EN 12469 2000 Air velocities downflow e Switch on the cabinet e Measure the airspeeds according to the pattern of Figure 43 with an anemometer at a height of 100 mm above the front opening See Figure 42 This measurement must be done for at least one minute on each position Air velocities inflow e Switch on the cabine
63. ly a starting time but also an ending time for every programmed day Otherwise the UV will stay on until 00 00 h and only then turn off See also the Example on page 34 Example Programming the 7 days clock UV will be given as an example Switch on the UV Tuesday evening 22 00 and switch off the UV Wednesday morning 06 00 See 5 11 Hour counter information and reset FL UV Timer point 5a on page 32 for deleting all Hour counter programmed information Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Safe Cabinet in work mode Monday 11 06 33 MENU Socket zOff e Push button MEM Menu UM EFAB EN10 doc 34 EuroFlow EF and EF B Biological Safety Cabinet Set up for 7 days clock UV shows cm Mehstar A z Hour counter information B Status cabinet C gt Settings A Set up UV 7 days clock Function Clock e Push button A UV Function Disabled Disable Set up the UV 7 days clock e Push button NE Clock for setting up the UV 7 days clock Saturday UV on 00 00 UV off 00 00 Change e Push button HE Change for changing the start and end time Saturday UV on 00 00 UV off 00 00 A e The value can be changed by pushing button EXE gt gt Ex or Es Set up UV starting time Tuesday evening 22 00 3 Change start day Change start hour 22 00 Change start min 22 00 Change s
64. n page 79 Check visual if the air direction of the inflow is in direction of the front aperture and laminar over the whole area of the front aperture by the use of smoke Check visual if the air direction of the downflow is downwards in direction of the work surface and laminar over the whole work surface by the use of smoke UM EFAB EN10 doc 60 EuroFlow EF and EF B Biological Safety Cabinet 9 Technical specifications 9 1 Physical surrounding e M Clean elstar A EF4 EF 5 EF 6 EF B4 EF B6 Transport physics Surrounding temperature C From 25 up to 55 From s upto From es up to From z up to From z up to Surrounding temperature C From 25 upto 75 Frem 25 up to From 25 up to From 25 up to From 25 up to max 24 h p 75 75 75 75 Humidity 20 90 20 90 20 90 20 90 20 90 In use physics EE EF 5 EF 6 EF B4 EF B6 Surrounding temperature C 15 30 15 30 15 30 15 30 15 30 Humidity 76 30 80 30 80 30 80 30 80 30 80 9 2 General specifications Dimensions EF4 EF5 EF6 EF B 4 EF B 6 mm mode toi di 163x1120 165x1495 165x1800 165x1190 165x1800 i mm rm maximum 350 350 350 350 350 Exteriors dimensions mm without support frame 1333x844x 1552 1943x844x 1552 1333x844x1552 1638x844x1552 1943x844x1552 without exhaust hood 650 650 Ixdxh Height with support mm frame for work height 2297 2297 2297 2
65. nded to use an in height adjustable well disinfectable chair to optimize the work position 4 15 Front panel The service panel Figure 3 Figure 4 Figure 5 and Figure 6 Pos 15 can be dismantled to get access to e the downflow filter UM EFAB EN10 doc 16 Clean Waste EuroFlow EF and EF B Biological Safety Cabinet e the fan GENERAL DANGER BI OLOGI CAL DANGER Cabinet must be disinfected before removing the front panel 4 16 Back panel The back panel Figure 3 Figure 4 Figure 5 and Figure 6 Pos 16 can be dismantled to get access to e optional fittings e sockets GENERAL DANGER BI OLOGI CAL DANGER Cabinet must be disinfected before removing the back panel 4 17 Main power connection The main power connection Figure 3 Figure 4 Figure 5 and Figure 6 Pos 17 is positioned on the top of the cabinet The cord length is maximum 3 meters and should always be accessible See 9 2 General specifications on page 61 for details 4 18 Electronics The electronics Figure 3 Figure 4 Figure 5 and Figure 6 Pos 18 consist of e Operational print positioned in the top hood behind the operating panel e Control print positioned under the top hood behind the safety cover including o Fuse holders see 49 2 General specifications on page 61 for fuse numbers o Fancontrol The control is free adjustable in the range between 096 and 10096 o An Air velocity compens
66. net e The connection that is located outside the installation is standard assembled in the left or right side panel of the installation e The connection that is located outside the installation can be assembled by customer request in top of the installation For EF B series assembly of this connection on the bottom is not possible Bunsen burner lead through set The Bunsen burner can be plugged into the outlet with a short cable inside the work space The plug of the foot pedal can be inserted into an outlet The Bunsen burner can only operate when the cabinet is in safe work mode and button SEIS Gas is activated Recommended to apply is the Fuego Bunsen burner available at Clean Air Techniek B V Positions of the supply connection EF e The connection that is located inside the installation is standard assembled in the back panel of the workspace e The connection that is located outside the installation is standard assembled in the bottom of the installation e The connection that is located outside the installation can be assembled by customer request in left or right side panel or top of the installation Positions of the supply connection EF B e The connection that is located inside the installation is standard assembled in the back panel of the workspace e The connection that is located outside the installation is standard assembled in the left or right side panel of the installation e The connection that is
67. ngs more details are given about UM EFAB EN10 doc 42 iologi i Clean Ar Telstar EuroFlow EF and EF B Biological Safety Cabinet a Preparations for disinfection Activating the user code Factory adjustments Testing of alarms Change the user code Change language Disinfection Your Biological Safety Cabinet has a built in disinfection software program This program knows a number of varieties which are dependent on the installed options in the security cabinet and the manner the cabinet has been installed The disinfection software of the control print is only available in version number 2 4 270307 or higher The disinfection software itself is no disinfection The Biological Safety Cabinet EF S can work according to automatic disinfection software only if this has been installed Consult your order affirmative for the installed options or contact Clean Air B V In case additional information about Disinfection is needed please contact Clean Air B V Also check the site of Clean Air B V www cleanair eu products downloads for more detailed information about disinfection Depending on the code of the installation the safety cabinet will react differently These codes are programmed for an optimised disinfection process The code itself is already pre installed on the cabinet depending on the installed options mentioned in your order affirmative or contact Clean Air Techniek e Check if the cabinet is gastight e U
68. nsible examiner can decide if this procedure is valid or not Additional standard operation procedures can be necessary 8 1 Periodic maintenance A periodic maintenance has to take place regularly To a certain extent this depends on the frequency of use of the cabinet Contact your service organization for advice Guideline for testing and maintenance given by Clean Air Techniek B V is every 12 months at least Validate the system on safety Check for correct functioning of the electrical system Check the HEPA filter for efficiency and leakage Check the installation and the exhaust system for leakage Verification calibration of the assembled air speed sensor s Check and adjust the alarm system Check if the air direction of the inflow is in direction of the front aperture and laminar over the whole area of the front aperture Check if the air direction of the downflow is downwards in direction of the work surface and laminar over the whole work surface 8 2 Replacement of the pre filter See 4 9 Drip tray pre filter on page 16 Tip This procedure must be done with a running fan Then the under pressure in the working area will stay This is an extra safety aspect Since this procedure can be done in a short time the extra pollution of the HEPA filters will be low This procedure is described for a right hand orientated person The described left hand may be changed for a right hand and right for le
69. ntact the manufacturer or the seller of the products UM EFAB EN10 doc 9 M M A Clean A Telstar EuroFlow EF and EF B Biological Safety Cabinet 2 5 Cytotoxic danger EF B only Before starting work in cytotoxics production the hazard risks of cytotoxics handling need to be evaluated and documented industrial safety act hazardous substances regulations Based on these findings the employees must be instructed 2 6 Explanation user safety symbols GENERAL DANGER You can harm yourself and others seriously if you do not follow the procedures carefully DANGER FOR ELECTROCUTI ON BI OLOGI CAL DANGER DANGER OF EXPLOSI ON DANGER BY I NDUSTRI AL VEHI CLE CYTOTOXI C DANGER PPPPPP UM EFAB EN10 doc 10 M M Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet 3 Installation GENERAL DANGER Be careful high centre of gravity crossover point Never hinge the window as long as the cabinet is not placed on the support frame The cabinet must be installed in a safe way Clean Air Techniek B V supplies an optional support frame which is designed for this purpose When the cabinet is purchased without a support frame it is advised to discuss the installation with the supplier 3 1 Assembly instruction The cabinet has to be placed on the support frame recommended by the supplier The assembly needs to be done as specified in instruction unde
70. ol sockets 31 5 11 Menu functions 31 5 12 General Information 32 5 13 Information status cabinet 40 5 14 Settings 42 6 Alarms J L LLL L L LLL LL LL LL LLL LLL 47 6 1 Most frequent window alarms 47 6 2 Flow alarms 48 6 3 Other alarms 49 7 Cleaning LLL 51 7 1 Cleaning the installation 51 7 2 Cleaning the UV light optional 53 7 3 Guideline for cleaning of cytotoxic contaminated surfaces EF B only 54 8 Small maintenance and testing 56 8 1 Periodic maintenance 56 8 2 Replacement of the pre filter 56 8 3 Replacing the Fluorescent light FL light 59 8 4 Service contact 59 8 5 Service Contract 60 8 6 Testing 60 9 Technical specifications 61 9 1 Physical surrounding 61 9 2 General specifications 61 10 Troubleshooting 65 Appendix l Support frames EF amp EF B 66 Appendixll Disinfection 67 Appendix III Replacement of the HEPA filters 68 1 When does a HEPA filter need to be replaced 68 2 Replacement of HEPA filters 69 Appendix IV Efficiency test of the HEPAfilters 74 UM EFAB EN10 doc 4 Clean EuroFlow EF and EF B Biological Safety Cabinet Requirements Istar 74 Appendix V Measuring method air velocities Air velocities downflow 76 76 Air velocities inflow 76 Appendix VI Lay out board
71. onnections are hose connections suitable for hoses with an inner diameter of 10 mm All connections on the gas supply have to be done with a certified gas hose After the connection on the gas supply the gas connections have to be tested on leakage by a certified installer An electrical gas valve has to be assembled on the gas connection see Annex A 9 of the EN 12469 2000 standard as an extra safety precaution A coupling via the electrical system of the UM EFAB EN10 doc 18 ee amp m a Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet installation makes sure that the valve can only be operated in work mode and will be switched off automatically by leaving this mode The gas valve switch function E only visible when the fan in work mode is and there are no air speeds alarms Positions of the supply connection EF e The connection that is located inside the installation is standard assembled on the back part inside the work space of the biological safety cabinet e The connection that is located outside the installation is standard assembled in the bottom of the installation e The connection that is located outside the installation can be assembled by customer request in left or right side panel or top of the installation Positions of the supply connection EF B e The connection that is located inside the installation is standard assembled on the back part inside the work space of the biological safety cabi
72. oth the swivel blocks See Figure 10 Pos 5 en Figure 15 so that the recesses point forwards UM EFAB EN10 doc 23 EuroFlow EF and EF B Biological Safety Cabinet c Netstar e Hinge tilt up the window see Figure 10 Pos 6 with the aid of the springs it stays open on the maximum position please use both hands for lifting the window OY window Rem E 5 Swivel block with recesses pointing forward 6 Fold up the sliding Figure 12 Hinge position Figure 13 Slide position Figure 14 Swivel blocks Figure 15 Hinge position Figure 16 Slide position After hinge actions follow reverse procedure to close and slide the window Only do not forget to pull both the locking pins out and turn 90 degrees see Figure 13 and also position both the swivels in the slide positions see Figure 16 UM EFAB EN10 doc 24 M Clean A Telstar EuroFlow EF and EF B Biological Safety Cabinet s ee 5 3 Display interface Figure 17 Display Interface The operating panel Figure 4 and Figure 6 Pos 19 and Figure 17 consists of 1 Display 2 Function keys 4x 3 Fixed keys 4x 4 Signals Display The display has 4 lines the lowest line gives the description of the function key underneath Cabinet switched off not safe II Monday 11 06 33 III IV Socket Of f Fixed keys EF Lighting ON OFF Work mode fan ON OFF Power save mo
73. ower supply so that the cabinet can be cut off of the power circuit 3 2 Transport instructions Before moving the cabinet make sure the power cable is disconnected of the power supply The cabinet must be transported on a strong solid pallet After every transport the cabinet must be tested and calibrated GENERAL DANGER Be careful High center of gravity crossover point 3 3 Dismantling amp Disposal For dismantling and disposal follow the next instructions e See 7 1 Cleaning the installation on page 51 See 8 2 Replacement of the pre filter on page 56 See Appendix II Disinfection on page 67 See Appendix III Replacement of the HEPA filters on page 68 Break the unit down into its component parts You can re cycle these components parts dispose them in accordance with local requirements For advice or information about dismantling or disposal please contact Clean Air Techniek B V UM EFAB EN10 doc 12 EuroFlow EF and EF B Biological Safety Cabinet 4 Product description ry Mass 2 8 0X1 19 Figure 5 Sectional view EF B Figure 6 3D view EF B UM EFAB EN10 doc M M Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet Part list EF and EF B Type plate Short user manual Body of the cabinet Hinge slide window Top hood Worktop Aerofoil Plenum construction Drip tray pre f
74. p a Telstar company Clean Air Telstar We make it possible User manual English version 10 0 valid for software V4 9 and higher Series EuroFlow Type EF Biological Safety Cabinet Class II amp Type EF B Biological Safety Cabinet Class II Types EF 4 EF 5 EF 6 EF B 4 EF B 6 Offers protection for personnel environment and product Before using the safety cabinet please read this manual carefully The required instructions have to be carried out first Clean Air Techniek B V Kuipersweg 37 3449 JA Woerden Nederland t 31 0 348 41 11 14 f 31 0 348 42 26 84 e infoQcleanair eu i www cleanair eu No M a Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet Preface Congratulations with your new biological safety cabinet Class II Series EuroFlow type EF and or type EF B This Dutch quality product is designed produced and tested by Clean Air Techniek B V in our production facility in Woerden The Netherlands The primary function of the safety cabinet is safety for operator product and environment Basic principle of the design are the latest standards regarding biological safety Type EF meets all the latest requirements including the European standard EN 12469 2000 for Microbiological Safety Cabinets Type EF B meets all the latest requirements including the European standard EN 12469 2000 for Microbiological Safety Cabinets and DIN 12980 2005 06 for Safe
75. particles out of the casing 7 When homogenizers are used in the installation all reservoirs have to be secured by screw connections so that a sudden emission of fluids or aerosols will be avoided To protect the personnel glass reservoirs are suitable for protection High speed homogenizers may cause air turbulence as do centrifuges 8 Even with the best technology there may still be a risk that the hands and arms of the personnel get contaminated during the work Movements shall be kept to a minimum Protective clothing shall have tightly fitting cuffs The use of hand gloves and over sleeves is recommended 9 Always use a chair with the correct height This enables the personnel to have a good view through the sliding window User should sit in such a position that looking under the edge of the sliding window during the work is not possible 10 It is important to keep the biological safety cabinet clean Minor contaminations are not always immediately visible and could easily be forgotten This increases the possibility that potentially infective material is left behind and may cause serious contamination of the interior of the installation or worse Regularly clean the worktop the bottom side as well as the space under the worktop in particular In case of doubt consider disinfection or contact Clean Air Techniek B V about the possibilities 11 Transport and installation of the cabinet is only allowed for authorized staff Please co
76. production controller Woerden date illlllevlreerrrreertreetrtteaassootastososonaonoon UM EFAB EN10 doc 95 ee Ne m Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet Appendix XVI Memo UM EFAB EN10 doc 96 EuroFlow EF and EF B Biological Safety Cabinet I ndex A Aerofoil 15 Alarm situations in work position 49 Alarms 47 Alarm test procedure 79 Assembly instruction 11 Authorized persons 8 B Back panel 17 Body of the cabinet 14 Bunsen burner lead through set 19 C Change language 46 Change the user code 88 Cleaning the aerofoil 51 Cleaning the inside of the window 53 Cleaning the installation 51 Cleaning the worktop 52 Clock 7 days 34 Code alarmtest 79 Control 22 Control board 78 Control Gas supply 30 Control sockets 31 Control UV 20 29 cytostatics 7 Cytotoxic 82 83 Cytotoxic danger 10 51 54 56 68 74 76 D Default code 26 DIN 12980 2005 90 Disinfection 67 Dismantling and disposal 12 Display 25 Downflow filter 16 Downflow HEPA filter 6 7 Drip tray 16 56 E Electronics 17 Emergency code 87 EN 12469 2000 89 Exhaust filter 16 Exhaust HEPA filter 6 7 F Factory adjustments 44 Fan 6 18 Feet Support 16 Fixed keys 25 UM EFAB EN10 doc Clean Wis FL light 59 FL lighting 18 Front panel 16 Function keys 25 Er Gas connection 18 Gas supply off 30 Gas supply on 30 General
77. r Cabinet in work mode See 5 4 Starting up the cabinet on page 26 Cabinet in work mode Safe Monday 11 06 33 1 Socket exi f e Push button BIB Menu A Hour counter information B Status cabinet 2 C Settings Hour counter display shows 3 Hour counter information shows A Push button Cabinet in use 38 Ne FIL i eos WE 22 4 B UV in use 0 00 zm Reset A Reset B FL Fluoresced lamp There are three different possibilities see 5a 5b and 5c Reset all timer and clock adjustments 5a pou e Push button BEI Reset hour counter and clock UM EFAB EN10 doc 32 Poen ac Netstar EuroFlow EF and EF B Biological Safety Cabinet e Push button En Reset e Push 2x button ENB Esc for starting menu Reset FL counter e Push button reset A 5b e Push button NE B Reset e Push 2x button amp ES Esc for starting menu Reset UV counter e Push button EE reset B 5c e Push button NE B Reset e Push 2x button EB Esc for starting menu Set up 7 days clock UV Cabinet in work mode f Cabinet in work mode Safe See 5 4 Starting up the cabinet WT ene e on page 26 MENU Soe keki e Push button MEM Menu A gt Hour counter information B gt Status cabinet C gt Settings A 1 pou e Push button KE gt Set up for 7 days clock UV shows e Push button A UV Function Di
78. r for EF B 6 e Semi facial mask with HEPA filters e Tools knife spanner or socket wrench alien key e Tape e Chemical disposal container or chemical disposal procedure Starting e Decontaminate the cabinet e Switch on the installation e Make sure that the window is the working position and that there are no alarms e Put on new gloves and keep an extra pair ready for use e Put on your breathe protection e g with a semi facial mask with HEPA filters e Hinge the worktop to the back partition e Remove the pre filter see 8 2 Replacement of the pre filter on page 56 e Remove the wire mesh frame of the pre filter Per filter pressure frame containing 2 HEPA filters the following procedure has to repeated for the EF B 4 with 4 pressure frames containing 8 HEPA filters this procedure has to be repeated 4 times For the EF B 6 this procedure has to be repeated 6 times 6 pressure frames and 12 HEPA filters UM EFAB EN10 doc 72 EuroFlow EF and EF B Biological Safety Cabinet Clean Maus Removing the old 1 HEPA filter sections of a pressure frame Remove the pressure frame of a filter section Seal both the openings of the two HEPA filters with DUCT tape or similar in order to isolate the contaminated side of these two HEPA filters from the environment Remove the first HEPA filter and put it in an appropriate plastic bag Fold back the edge of the plastic bag not touching the inner side of the plastic bag
79. r of the software used in the safety cabinet Software Version Cabinet in work mode Cabinet in work mode Safe See 5 4 Starting up the cabinet Monday 11 06 33 1 on page 26 MENU Socket Of f Software version e Push button Gr Menu e Push button EB 0 e Push 2x button ENB gt Factory Adjustments Vansi oms Kok OOO 110 VXXXX OPT 8 5 Service Contract For information and orders concerning service or service contracts please contact Clean Air Techniek B V 6 6 Testing Recommended tests for first installation or after changing the HEPA filters are Velocity tests see Appendix V Measuring method air velocities on page 76 Filter Integrity tests see Appendix IV Efficiency test of the HEPA filters on page 74 Alarm tests see Appendix VII Alarm test procedure on page 79 Check visual if the air direction of the inflow is in direction of the front aperture and laminar over the whole area of the front aperture by the use of smoke Check visual if the air direction of the downflow is downwards in direction of the work surface and laminar over the whole work surface by the use of smoke Recommended tests after installing with a regular interval are Velocity tests see Appendix V Measuring method air velocities on page 76 Filter Integrity tests see Appendix IV Efficiency test of the HEPA filters on page 74 Alarm tests see Appendix VII Alarm test procedure o
80. re is no danger for the service engineer labour personnel the laboratory and the surroundings of the cabinet If necessary disinfect the installation For more information Appendix II Disinfection on page 67 CYTOTOXI C DANGER When cytotoxics are used see 2 5 Cytotoxic danger EF B only on page 10 Only the responsible safety officer biological safety officer or the responsible examiner can decide if this procedure is valid or not Additional standard operation procedures can be necessary The valid procedures for the disposal of possible contaminated material must always be followed When the service engineer thinks this is not enough this will be discussed with the responsible person so that enough precautions can be made Clean Air Techniek B V strongly recommends specially trained technicians to carry out filter exchange Certified service organizations are recommended At all times keep to the service and maintenance procedures from Clean Air Techniek B V 1 When does a HEPA filter need to be replaced Under normal circumstances the serviceable life time of a HEPA filter is several years mostly between 5 and 10 years The filter should be replaced whenever the air velocity and or the filter Integrity are no longer in compliance with the EN 12469 2000 The cabinet has a microprocessor that is automatically regulating the fan speed to compensate for main power fluctuations and filter pollution Downflow H
81. rneath Assembly support frame Build the support frame see Appendix I Support frames EF amp EF B on page 66 for an explaining drawing e Mount the support yoke Pos 1 to the reinforcement plate Pos 3 e Mount optional feet support Pos 2 e tem no 6 is used to position the cabinet to the support frame see 3 1 Placing the cabinet on page 11 Placing the cabinet e Place the cabinet right on the forks of a forklift truck or lifting platform DANGER BY I NDUSTRI AL VEHI CLE Be careful Make sure the cabinet cannot slide from the forks e Move the cabinet to the correct height and position perform this action as precise as possible above the support frame e The weight must be equally divided between the both forks and the cabinet must be horizontal levelled e The cabinet should be aligned with the covers of the support frame The two cones on top of the yokes will help aligning e Mount the cabinet to the support frame by means of the four inner hexagonal cylinder screws M8x12 Pos 6 See Appendix I Support frames EF amp EF B on page 66 e Connect the wire to a grounded socket that is easy accessible UM EFAB EN10 doc 11 Clean Wekt EuroFlow EF and EF B Biological Safety Cabinet In case of maintenance the cabinet must be completely free of voltage This means that the connector in the socket must be easy to reach otherwise a main switch must be mounted between the cabinet and the p
82. rt audible signals will be heard to be remembered at the initial unsafe mode After 30 minutes after the last button is touched the fan returns to its work mode Alarm test procedure Cabinet in work mode Cabinet in work mode Safe See 5 4 Starting up the cabinet Monday 11 06 33 on page 26 MENU Socket Of f e Push button SE Menu A gt Hour counter information B gt Status cabinet gt Seuil was A e Push button ENB C Pou Alarm test e Push 3x button NE gt gt Testing of alarms Testing Alarms 0000 Enter code e Enter the test code 7733 e Push button Alarm test settings NM Normal mode PS Power save mode DF Downflow IF Off 0 00 PS 0 14 ORDRE MONEO aI IF Inflow CV Current value NM 500 Off 0 PS 300 CV 490 DF Off 0 00 PS 0 19 0 30 0 37 0 44 e Push button BEI or BEI in order to adjust the fan speed Value CV will change UM EFAB EN10 doc 79 ee M M a Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet Middle numbers are the actual values from the airspeed indicators which are changeable with the use of the buttons BEI ana B First and last values are the set up alarm borders for example Downflow DF lowest level is 0 30 m s highest level is 0 44 m s and the actual value for downflow velocity is 0 37 m s The fan speed is shown in tenths of percents 0 min and 100
83. s BI OLOGI CAL DANGER BE CAREFUL Before starting service with the cabinet a written declaration that the cabinet is decontaminated must be present The responsible person laboratory supervisor will decide if the cabinet has to be decontaminated so that when servicing the installation and the HEPA filters there is no danger for the service engineer labour personnel the laboratory and the surroundings of the cabinet If necessary disinfect the installation For more information Appendix II Disinfection on page 67 CYTOTOXI C DANGER When cytotoxics are used see 2 5 Cytotoxic danger EF B only on page 10 Only the responsible safety officer biological safety officer or the responsible examiner can decide if this procedure is valid or not Additional standard operation procedures can be necessary Requirements According to the norm EN 12469 2000 HEPA filters must meet a few requirements Efficiency test by means of a particle counter The downflow exhaust and first filter must have an efficiency of 99 995 A locally measured value can be a factor 10 higher lt 0 05 Efficiency test with a photometer The downflow exhaust and first filter must have an efficiency of 99 9996 Any locally measured value must be x 0 01 When a HEPA filter does not meet the EN 12469 2000 it is allowed to repair it However the surface of the reparation must not be more than 5 of the total surface of the HEPA fi
84. s Appendix VII Alarm test procedure Appendix VIII Stickers Appendix IX Information installed options Exhaust hood 78 79 81 84 84 Installed options microprocessor 84 Appendix X User code Activating the user code 87 87 Changing the user code 88 Appendix XI Required specifications Overview of the required specifications according to EN 12469 2000 89 89 Overview of the required specifications according to DIN 12980 2005 90 Appendix XI I Part list critical components Appendix XIII Short user manuals UM EFAB EN10 doc 91 93 94 95 96 97 EuroFlow EF and EF B Biological Safety Cabinet 1 Introduction l 1 EF Clean pO The EF is a class II Biological Safety Cabinet It gives the user the possibility to work safely and sterile with bioagentia without complete physical barrier between person and experiment through work opening C Working surface A is kept clear of particles by means of a clean downflow of air Because of the downflow air passed through a HEPA filter High Efficiency Particulate Air the working area of the cabinet is free of particles This airflow is laminar the airflow has got no turbulence and has got a constant air speed Through the work opening C air is drawn into the workspace inflow towards the front slots of the worktop This protects the personnel against pathogenic aerosols that might be released The downflow and inflow air
85. sabled Disable There are three ways to set the UV 2a Timer 2b 7 days clock UM EFAB EN10 doc 33 EuroFlow EF and EF B Biological Safety Cabinet 2c Disable Waste Clean A Timer The timer sets a period until the UV turns off Push button EN Timer to set up the timer e Push button BEI Change to change the time e The value can be changed by pushing button En 2a gt gt E or EB e Acknowledge the setting with button f e Push 2x button B Esc for starting menu Clock 7 days clock 2b With the clock function for each day of the week you can set a variable period for the UV to be switched on Keep in mind you have to set the starting hour and starting minute for each day separately For detailed information regarding programming this menu see 5 11 Set up 7 days clock UV on page 33 e Push button MB Clock to set up the 7 days clock Push button SES Change to change starting time en ending time e The value i can be changed by pushing button En gt gt E or EB e Acknowledge the setting with button A e Push 2x button ENB Esc for starting menu See also the Example on page 34 Disable 2c By pushing on Disable the UV can only be turned on and off manually e Push button BEI Disable to set the UV manually e Push 2x button SESS Esc for starting menu Please select not on
86. safety aspects 9 H Hinge slide window 15 Hour counter info and reset 32 34 l Information status cabinet 40 Installation 11 Installed options 41 84 86 Interface board 78 L Lay out boards 78 M Main power connection 17 Maintenance and repair general 8 Measuring level inflow 77 Measuring method air velocities 76 Measuring points downflow 77 Measuring points inflow 77 Menu functions 32 N Night mode 28 0 Operating panel 18 Operating the installation 8 Optional Fittings 18 p Part list critical components 91 Particle counter 74 Periodic maintenance 56 Photometer 74 Placing the cabinet 11 Plenum construction 15 Power save mode 28 Pre filter 6 16 Procedure in case of alarms 50 Product description 13 Protection grid 16 R Replacement of the Downflow HEPA filter 70 71 72 Replacement of the HEPA filters 68 Replacement of the pre filter 56 97 EuroFlow EF and EF B Biological Safety Cabinet Replacing the Fluorescent light 59 S Safety 8 Safety symbols 10 Screen I 42 84 Screen II 42 85 Screen III 42 86 Service contact 59 Service Contract 60 Set up 7 days clock UV 33 Set up actual time 39 Set up cabinet 7 days clock 38 Set up socket 7 days clock 37 Short user manual 14 93 Signals 25 Slide window 23 Sliding window 22 Socket off 31 Socket on 31 Sockets 21 Software
87. se the valid standard operation procedure SOP since the disinfection procedure depends on the handlings performed in the cabinet Cabinet in work mode Cabinet in work mode Safe See 5 4 Starting up the cabinet Monday 11 06 33 on page 26 MENU Socket Of f e Switch off the cabinet with button a e Push button EM Menu A Hour counter information Y e Seis EO Met C Settings A e Push button NEN C e Push button Preparations for disinfection Preparations for disinfection 0000 Enter code Preparation for disinfection display shows e After entering the user code pre settings 0001 push UM EFAB EN10 doc 43 ZAN X a Clean Aic Telstar EuroFlow EF and EF B Biological Safety Cabinet button A e Push button BEI check OK The aerofoil has to be removed out of the work area and the window has to be closed completely The valve on the exhaust if present has to closed e Push button MEM start e Re enter the user code e Push button The preparation for the disinfection starts 3 Abort the disinfection e Push button EI Stop to stop the disinfection For more information about disinfection see Appendix IT Disinfection on page 67 and The disinfection software itself is no disinfection website www cleanair eu Products Downloads Activating the user code See Appendix X User code on page 87 Factory adjustments
88. sing it 7 3 Guideline for cleaning of cytotoxic contaminated surfaces EF B only CYTOTOXI C DANGER When cytotoxics are used see 2 5 Cytotoxic danger EF B only on page 10 Only the responsible safety officer biological safety officer or the responsible examiner can decide if this procedure is valid or not Additional standard operation procedures can be necessary In the contract of employment agreement of Dutch hospitals of June 2004 a guideline is given for cleaning of cytotoxic contaminated surfaces Summary of this quideline Cabinets contaminated with cytotoxics are normally cleaned with a 1 solution of Natriumdodecylsulfat SDS solution CAS no 151 21 3 in demineralised water as described in 7 1 Cleaning the installation on page 51 In addition to this cleaning procedure it is recommended to clean the cabinets surfaces with a 70 Ethyl alcohol solution ethanol CAS no 64 17 5 This can be necessary to ensure sterile conditions for example against bioagentia To complete this process gas disinfection can be necessary Exceptions A few cytotoxics have a different cleaning procedure Example 1 Cytotoxic CAS no Recommended cleaner Azathioprine 446 86 6 Bleomycine 11056 06 7 Daunorubicine Daunomycin 20830 81 3 Doxorubicine 23214 92 8 Epirubicine 56420 45 2 250 ppm Hypochloriet Sodium Fluorouracil 51 21 8 Hypochlorite CAS no 7681 Idarubic
89. ssembled on the back wall of the working area Figure 3 Figure 4 Figure 5 and Figure 6 Pos 22 and 23 They can be operated at the control panel See 49 2 General specifications on page 61 for electrical details The constructions of these may vary per country When the Biological Safety Cabinet has been switched off this function has to be activated each time via the switch on the operating panel Please contact Clean Air Techniek B V for information about alternative options 4 24 Housing of the first HEPA filter EF B only The EF B has a number of V shaped HEPA filters Figure 6 Pos 24 These filters 8 for the EF B 4 and 12 for the EF B 6 are mounted in a filter container underneath the working space 4 25 First HEPA filters EF B only The EF B has a number of V shaped HEPA filters These filters 8 for the EF B 4 and 12 for the EF B 6 are mounted in a filter container Figure 5 Pos 25 underneath the working space UM EFAB EN10 doc 21 Clean M estar EuroFlow EF and EF B Biological Safety Cabinet 5 Control 5 1 Using the cabinet Check if the information on the type plate is corresponding to the main power connection Connect the cabinet to the main connection by putting the plug into a grounded socket that is easy accessible 5 2 Top hood sliding window The hinge slide window can be in several positions see Figure 8 Window positions The used example of the EF is also valid for the EF B Hinged
90. star Component Type Art no Supplier Type downflow Air velocity sensor 1330010 Kriwan 0 2 10 m s exhaust inflow a FL holder All 1440110 BJB BJB G13 Starters All 2040001 Philips PH starter S10 220 240V 4 65W Miniature switch All 63001P1 Omron SS 5GL2 Main filter All 6400010 Acrotronics F BK D 3940 ZG 10A PCB material All Duraver FR 4 according to UL94 Socket All Misc e g Mennekes Schuko Optional Gas vacuum tap All 2800013 2800048 Broen 1770 Gas valve All 2820012 Burkert 0 25 Bar 2 2 way valve normally closed 24 V Bunsenburner All Misc Clean Air 1 SCS Lumos II 9V or Fuego Articles mentioned above can be ordered at the service organization Particle Measurement amp Validation b v Particle Measurement amp Validation b v Kuipersweg 37 3449 JA Woerden The Netherlands UM EFAB EN10 doc Phone 31 0 348 423661 Fax 31 0 348 422684 E mail info pmvbv nl Internet www pmvbv nl 92 reistar Clean Ar EuroFlow EF and EF B Biological Safety Cabinet Short user manuals Appendix XIII 20p TON3 Wf SHOWA XX A43 uoneuuojui eususpxe Joj jenuey SN ses 943 ed wo 4o1n3 eues UTJA JAND poo day ayi 350 uonisod Guppom oui opui ay apis eq sejijand epis ayi Gury pue HPA janms eu asop pue sud GUDO au xoojur opui ayi umow vaym sdubpueu Oq esn sey uonpnijsuoo MOPUIM au JEAN Sbuig opu ayi BUISOID MOPUIM aui BUIA
91. t e Measure with an anemometer the average air velocity in the inflow according to the pattern of Figure 41 and with probe position as shown in Figure 40 e Multiply these numbers with the surface of the exhaust opening the result is the air volume through the safety cabinet e Divide the air velocity through the surface of the front opening the result is the average value of the inflow air speed UM EFAB EN10 doc 76 NI Pe A Clean A Telstar EuroFlow EF and EF B Biological Safety Cabinet Figure 40 Side view for measuring level inflow Measuring probe Figure 41 Measuring points inflow H Height B Broad xl The average inflow velocity at the top of the front aperture is measured and can be read in the display The minimal required value of the average inflow velocity measured at the top of the front aperture is given in chapter 9 2 on page 61 Measuring these most critical measured values directly under the window ensures that the overall average inflow velocity is according to the guidelines of the EN12469 2000 Annex H table H1 Mean inflow velocity to achieve operator protection gt 0 4 m s Figure 42 Side view for measuring level downflow i i BACKPANEL I I I i 1 I 4D da JAZ 500000 TAB da o o I a PONTSAT MEASURE LEVEL Lo ABI 00000 L ea ips iB4 1 I 4D 1 FRONT WINDOW 1 8 w 3 W 8 8 me
92. t air slits of the working surface 6 3 Other alarms Other alarms are Fan failure Figure 24 Most likely the fuse is broken Replace it with a new one If the alarm is still there contact your service organization To reset this alarm you must disconnect the power plug from the main voltage Communication error Communication error Figure 25 Contact your service organization Overview possible alarms Window Window above below No working working Downflow Downflow Inflow Inflow alarm position position too low too high toolow too high Person protection Product protection Yes No Yes Yes No No Yes Yes No No No No Table 1 Alarm situations in work position UM EFAB EN10 doc 49 EuroFlow EF and EF B Biological Safety Cabinet Procedure in case of alarms Clean A reistar Check if the window is in the working position When the window is back into the working position the alarm will not mute immediately The cabinet needs time to get the correct airflows In case the alarm is still present Stop working close the window and push button id The cabinet goes into the saving position Read the alarms from the display Contact your service organization Cabinet in work mode Not Safe Monday 11 06 33 MENU Socket Of f Working with the cabinet is not possible anymore UM EFAB EN10 doc 50 M M Clean Telstar EuroFlow EF and EF
93. t critical components on page 91 UM EFAB EN10 doc 64 EuroFlow EF and EF B Biological Safety Cabinet 10 Trouble shooting Fault Cause a M Clean elstar Remedy No power on socket of the cabinet Socket is not activated pey Activate socket on display push on EZAI Fuse is broken Replace the fuse UV button is not visible on display UV equipment is not installed X Option UV is not installed UV option has to be programmed please contact your service organization UV is not working Window not down Window completely downwards Gas button is not visible on display Gas equipment is not installed X Option gas is not installed UV option has to be programmed please contact your service organization Gas is not working Fan in off position Fan in work mode High flow alarm Alarm in safe night mode FL light is not working Contacts of the FL are not locking in the Turn the FL light slightly power connections UV light is on Switch of the UV light Cabinet is locked User code must be entered Enter the user code or de activate the user code see 5 14 Change the user code on page 45 UM EFAB EN10 doc 65 Clean A Maus EuroFlow EF and EF B Biological Safety Cabinet Appendix Support frames EF amp EF B For every type of cabinet from the EF series and the EF B series a specific support frame is avai
94. the plastic bag on the aerofoil Pull the pre filter from its frame with your right hand Roll the filter up still with your right hand Put the filter into the plastic bag UM EFAB EN10 doc 58 Clean Masa EuroFlow EF and EF B Biological Safety Cabinet Figure 33 Put the filter into the bag Fold back the edge of the plastic bag not touching the inner side of the plastic bag Press the air out of the bag with its opening still in the working space Seal the plastic bag airtight Remove the plastic bag and place it in an appropriate container Put on new gloves Place a new pre filter in the frame Fold the worktop back in its position Remove the gloves and put them in the appropriate container 6 3 Replacing the Fluorescent light FL light When an FL light see 4 20 FL lighting on page 18 needs to be changed the top hood can be lifted Switch off the power of the installation Replace the FL tube by a new one see Figure 34 and close the top hood DANGER FOR ELECTROCUTI ON Figure 34 Position of the FL tubes 6 4 Service contact For advice or any technical questions please contact your service organisation Please refer to the following items e Type Class Project number and Serial number mentioned on the type plate UM EFAB EN10 doc 59 Clean A reistar EuroFlow EF and EF B Biological Safety Cabinet for the position of the type plate see page 14 and page 82 e Version numbe
95. the sockets are automatically switched off Also if the cabinet is switched from work mode into power save mode the sockets are automatically switched off See technical specification see 9 2 General specifications on page 61 for maximum voltage and current fuses Switching socket on Cabinet in work mode See 5 4 Starting up the cabinet 1 07 page 26 Cabinet in work mode Safe Monday 11 06 33 MENU Socket Of f The display shows Socket On Power of socket is switched on e Push button NE Socket Off Cabinet in work mode Safe 2 Monday 11 06 33 Socket switched on MENU Socket On Switching socket off A The display shows Socket Off e Push button BEI Socket On Power of socket is switched off Cabinet in work mode Safe 3 Monday 11 06 33 Socket switched off MENU Socket Of f 5 11 Menu functions From the main menu you can go to 5 12 General information 5 13 Information about the status of the cabinet and 5 14 information about the settings UM EFAB EN10 doc 31 EuroFlow EF and EF B Biological Safety Cabinet 5 12 General I nformation F can ac Nestar In general information more details are given about e Hour counter information and reset FL UV Timer Set up socket 7 days clock Set up cabinet 7 days clock Set Actual time Set up 7 days clock UV Example Hour counter information and reset FL UV Time
96. tion of the working space UM EFAB EN10 doc 52 NM mE Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet Figure 27 Fold the worktop against the back partition Clean the sides and bottom part of the worktop Fold the worktop back in place Cleaning the inside of the window Check if this procedure can be done safely It is advised to disinfect the installation first see Appendix II Disinfection on page 67 Procedure for cleaning and to disinfect the window with liquid Put on gloves Switch off the installation see 45 7 Turn off on page 28 Hinge the window open see 5 2 Hinge the window on page 22 Clean and if necessary disinfect the window Clean and if necessary disinfect the rubber seal Smear the seal thin with Vaseline Hinge the window close Please note the cleanability of the biological safety cabinet is at least conform Class CI B EN 12469 2000 table 2 Cleanability performance 7 2 Cleaning the UV light optional BI OLOGI CAL DANGER Never clean an used installation without precaution for instance disinfection DANGER FOR ELECTROCUTI ON UM EFAB EN10 doc 53 ee Ne m a Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet The UV light must be cleaned regularly with alcohol 7096 Put on gloves and switch of the cabinet and let the UV light cool down before cleaning After cleaning the UV light tube must be dried before u
97. tochemical processes Note Radiation of these lamps is harmful to eyes and skin Installations with these lamps are to be screened off completely U C Useful Depreciation radiation life 5000 hrs w h a7 15 li 21 34 5000 500 500 8000 58000 25 46 8000 8000 5000 5000 8000 5000 8000 5000 5000 8000 15 58000 15 8000 15 5000 15 500 15 500 15 114 145 Maintenance relason between UV C output UVC outpuin 94 Waste Clean EuroFlow EF and EF B Biological Safety Cabinet Appendix XV Statement of the agreement for machines Manufacturer Clean Air Techniek B V Address Kuipersweg 37 3449 JA Woerden The Netherlands Phone 31 0 348 411114 Fax 31 0 348 422684 E mail info cleanair eu Internet www cleanair eu Hereby declares that Type Project number Serial number is in compliance with the following directives e Machine Directive 2006 42 EC e Low Voltage Directive 2006 95 EC e Electromagnetic Compatibility Directive 2004 108 EC And declares furthermore that the following national technical standards and specifications are used e NEN EN 12469 2000 Performance criteria for microbiological safety cabinets e NEN EN 1822 High efficiency particulate air filters e DIN 12980 2005 Zytostatika Werkbanke additional national technical standard only for EF B 4 and EF B 6 Signature Names Asirans ete Quality controller By order of the
98. top hour 00 00 e Saturday has to be changed into Tuesday UV starting time at 22 00 and UV off at midnight 00 00 The display shows UM EFAB EN10 doc 35 EuroFlow EF and EF B Biological Safety Cabinet Change stop min 00 00 F can az Sreistar Tuesday UV on 22 00 UV off 00 00 Set up UV switching off on Wednesday morning 06 00 Change stop day Change start hour 00 00 Change start min 00 00 Change stop hour 06 00 Change stop min 06 00 Check of programmed UV Clock e Push button EN gt then button A then button BEN Clock and then button HEB Change e Tuesday has to be changed into Wednesday UV starting time at midnight 00 00 and UV off at 06 00 e The display shows Wednesday UV on 00 00 UV off 06 00 e Push 2x button MEMB Esc for starting menu e Push 3x button ME Menu e Push button am e Push button NE Clock for checking the UV clock day by day Wednesday UV on 00 00 UV off 00 00 e Push button NEN Change to see what has been programmed Wednesday UV on 22 00 UV off 00 00 UM EFAB EN10 doc 36 F can ac Nestar EuroFlow EF and EF B Biological Safety Cabinet pou e The value can be checked by pushing button En gt gt change day Every days program can be controlled by pushing MB or B e Important Don t touch only use 2x button En Esc to go back to the main men
99. ty Cabinets for cytotoxics The standards EN 12469 2000 and DIN 12980 2005 06 describe only the minimum requirements They serve as the base for the high demands Clean Air Techniek B V pursuits for its products Type EF and type EF B are high quality products constructed with high grade components and materials Besides providing biological safety the wishes and demands of the operators have been important basic principles for the design Examples are ergonomics design and usability The above mentioned motivations have resulted in the most progressive safety cabinets currently available known for their superior quality reliable performance solid construction and many available options accessories related products and the possibility to incorporate weighing systems microscopes etc or even build completely custom made cabinets Furthermore type EF and EF B are service friendly installations The safety cabinet is equipped with new techniques such as e Microprocessor control with a LCD display for the interface with the operator e Automatic up speeding fan when the filter resistance increases the necessary airspeeds are maintained e Window construction with a user friendly hinge mechanism We thank you for buying this cabinet and wish you good times working with the biological safety cabinet class II type EF and or type EF B For questions advice training validation service and product adjustments please contact us Clean Air T
100. u Set up socket 7 days clock Cabinet in work mode Cabinet in work mode Safe See 5 4 Starting up the Monday 11 06 33 cabinet on page 26 MENU Socket Of f e Push button SB Menu A gt Hour counter information B gt Status cabinet C se Seil mas A Set up socket 7 days clock Set up display for socket 7 days Function disabled clock shows e Acknowledge the setting with button A Socket function Disabled Disable UM EFAB EN10 doc 37 Na M Clean An Telstar EuroFlow EF and EF B Biological Safety Cabinet S M There are three ways to set the Sockets Timer 7 days clock Disable UV on page 33 and further 2 See also the Example for page 34 See 5 11 Set up 7 days clock programming the UV lamp on The timer function needs to be activated first otherwise the sockets will not switch on Set up cabinet 7 days clock With this menu it is possible to program an automatic switch action on of the cabinet It is however not possible to switch off the cabinet itself automatically Cabinet in work mode Cabinet in work mode Safe See 5 4 Starting up the Monday 11 06 33 cabinet on page 26 MENU Socket Of f e Push 5x button ME ix Menu 1 x A and 3x gt Setting cabinet 7 days clock Function disabled 1 e Acknowledge the setting with button A Tuesday Cabinet to work mode at 0
101. ust decide whether there are any Cytotoxic hazards or not In case of any Cytotoxic hazards the sticker has to positioned on the cabinet above the front aperture According to DIN 12980 2005 UM EFAB EN10 doc 82 NI TE Clean Telstar EuroFlow EF and EF B Biological Safety Cabinet 9 Cytotoxic only EF B This sticker is delivered with the cabinet Depending on the work that is done on the cabinet the customer must decide whether there are any Cytotoxic hazards or not In case of any Cytotoxic hazards the sticker has to positioned on the cabinet above the front aperture According to DIN 12980 2005 UM EFAB EN10 doc 83 EuroFlow EF amp EF B Biological Safety Cabinet Clean Air Techniek B V Clean i Appendix IX Information installed options Exhaust hood The exhaust hood is used in case the cabinet is connected to an external system On the hood a thimble a booster fan or duct can be positioned The exhaust hood creates an extra barrier and produces extra air resistance This extra resistance is in most of the cases compensated by additional external fan power In case the exhaust hood is not connected to an external system as described above the exhaust hood only produces extra resistance and will reduce the airflow in the exhaust chamber In this case it is recommended to remove the exhaust hood or contact Clean Air Techniek B V for advice For the same reason the exhaust channel may not be obstructed
102. w at the top side of the work aperture should be 0 28 m s e In case HEPA filter Integrity is measured with a photometer and DOP EMERY 3004 or DEHS as test aerosol then the maximum local penetration should not exceed 0 01 Yo If the required Integrity cannot be met then it is allowed to repair up to 5 of the surface area of the HEPA filter Alternative method In case HEPA filter Integrity is measured with a particle counter then local penetration should be lt 0 05 When the overall Integrity is measured the penetration should not exceed 0 005 Yo 2 Replacement of HEPA filters Make sure that the cabinet has been properly decontaminated so as to ensure that no danger exists for the service engineer the laboratory personnel and the environment If the customer has a special procedure for the disposal of contaminated material then this should be followed In case the service engineer does not think that this is sufficient then proper precautions should be made in consultation with the person responsible for the safety cabinet If there is no special procedure it is best to wear at least gloves and the protective clothing normally worn during work with the safety cabinet It is also advised to have a breathe protection e g with a semi facial mask with HEPA filters UM EFAB EN10 doc 69 Clean Wits EuroFlow EF and EF B Biological Safety Cabinet Take note of this warning and advice since these activities are potentially harmful
103. y Cabinet e They are sufficiently informed about the operation of the installation and the operation of the sub systems 2 4 General safety aspects 1 The installation shall never be used for any micro biological or any other potentially dangerous work unless the fan is switched into working mode the sliding window is in working position and there is no alarm 2 It is strongly advised not to slide the window out of the working position during working with the installation When it is necessary to increase the work opening this interruption needs to be as short as possible 3 When the installation is not used the window shall always be closed with the fan off or switched onto power save mode to prevent that dust particles etc from the room can get into the installation or worse material from the installation can get into the room 4 Always try to limit the amount of equipment in the workspace Each object in the workspace will disturb the pattern of the airflow and a large number of equipment may lead to serious disruptions 5 Materials such as Petri dishes or tissues should during activities never cover the slots of the worktop since no protection of the personnel can be guaranteed 6 Do not use a centrifuge in the workspace Even a small centrifuge is large in comparison with the work surface and its presence may seriously disrupt the airflow In addition all centrifuges themselves create an airflow which may fling

User manual English Series EuroFlow, Type EF Type EF/B

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