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CO2 UNIT BL - User Manual

1. AL OXO okolab CO2 UNIT BL Gas Controller bold line Manual This page was left blank 2 Index i REVISION TABLE Lilla 4 PREFACE luccica 5 SYMBOLS DESCRIPTIONS aa ias 6 3 1 1 Symbols in this manual and On the prodUCE id hi dada aten cad nas acc na 6 3 1 2 Symbols On the productlabDel arios teca 6 4 SAFETY NOTES iii 7 AC SECTION WIRING DIAGRAM sisissssvescsassvanssesivenivessnnsivanssententinsnsssssvenasessvannvessvantuncasastirvsiegeunsyvananesnvaniiettes 9 EQUIPMENT AND CONNECTIONS rrrrrrrrrrreeeeeeezesese cene eeee ceneri SSSR KRKA eee cene OSS Seen 11 6 1 Equipment supplied and Tubing detailS rr K K K K K K K K K K K K K K K K K K K K KKK 11 6 2 Equipment NOT supplied udal mou did iii ia 12 7 ILLUSTRATION OF THE MAIN UNITS 00000000000000000 0000 neon eee eee eee eee eee eee sese ceci ne nine ni Sne nn 12 7 1 COZ OZBE O10 0 ipa alal dalai rivalta 12 7 2 Pressure Gauge Sel UD And USE irreali 13 HOW TO ASSEMBLE C02 UNIT BL WITH A TEMPERATURE UNIT ccccccccccccccccccccononcccccncncnoos 14 CO2 UNIT BL INSTALLATION AND USER GUIDE ccccccccnnnnnonnncccccccccccnocoocccccoooooonononococoncconnccccnnonos 15 9 1 CO2 UNIT BL Installation Quide sorisa kanaa a 15 9 2 CO2 UNIT BL Set up the system and Turn The Unit On n eens 19 9 3 Unit operation through he TOUCH SClICON zsz ENEA EE E bes ususudsaa z sis 20 9 3 1 One piece 20 9 3 2 C
2. Figure 32 External Gas Meter Connection Connect the external Gas meter as described above see Figure 32 The Gas Meter will read Same gas levels that are going into the Top Stage Incubator Wait until the external Gas meter steadily reads CO value this may take 10 15 minutes When you are sure that the value being read is at steady state press on the External Probe Tab The screenshot shown on the right of Figure 33 appears Press on to input the CO2 level read by the external Gas meter i e 5 2 for CO2 then press Save Settings Gas Offset Settings Offset Bet Ref Value External Probe CO Unit Offset A NU 5 2 L Total Flow 0 400 lIfmin Cancel Reset Adj CO2 l Figure 33 Manual Calibration The Touch Screen will go back to the previous screen It will display on the left External Probe tab CO level read by the external Gas meter as you have just input The value in the CO2 Unit tab is the value that the device CO2 UNITL BL was reading before connecting it to the external Gas meter Now press Adj CO2 29 ee ca OX geninde E SAE okolab External Probe CO Unit Offset CO Pr 5 5 0 0 0 E Total Flow 0 400 Ifmin 9 10 15 AM Figure 34 Manual Calibration Correction Once you have pressed Adj CO2 the system will perform a self calibration using the input value from the external meter as a ref
3. Time Factory Reset Figure 18 How to enter in Alarms page Tip gt Flag Buzzer if you want the Alarm to be acoustical as well rather than just being displayed e okolab E El 5D Gas Deviation 0 5 Y Ez A Gas Time 10 minutes 10 15 AM Figure 19 How to set the Deviation and Time Alarm Settings Touch Screen Gas Alarms Figure 19 In this example the following settings are displayed valid only after the system has reached its stationary state if Gas Deviation from set point is 0 5 or greater i e if set point CO is 5 and CO reaches a value equal or less than 4 5 or equal or more than 5 5 for a period of time equal or longer than 10 minutes Gas time set in this example then the system triggers an Alarm 9 3 6 Gas Settings Temperature Settings are adjusted by following the two steps below look at the touching finger e okolab settings Touch Screen 23 Figure 20 Gas Settings When you press the Gas Icon the Gas Settings Submenu will open See Figure 21 Settings Air Source CO2 N Source Flowrates WPA A Gas cycles Status RM Figure 21 Gas Settings submenu 9 3 7 Air Source Within this window you can set which Air source you are using If this is an external Air tank or compressed Air line Compressed Air or if you are instead using the OKO AIR PUMP BL Air Pump See Figure 22 Le okolab o a Flowrates Ae
4. ve A E Compressed Air r sv Sensor Gas cycles Status calibration F a Fr n Fr a F an E Figure 22 Air Source page To connect the air pump OKO AIR PUMP BL to the CO2 Controller see Figure 10 In the case you are using OKO AIR PUMP BL please note that it can be turned on off only via the Touch Screen interface OKO TOUCH Thus it is essential that you hereby set the correct Air source 9 3 7 1 Flowrates This allows you to check how many liters per minute of Air and CO the system is consuming Additionally this panel allows you to select the flowrate of the gas mixture you re sending to 24 your incubator device Press the Total flowmeter set the gas flowrate in the range 0 0 0 8 L min using the slider settings Gas Change Total Flow Gas cycles Status Pi SA Figure 23 Flowrates View When setting the flowrate at 0 0 L min the gas flowrate will be paused and output flow will be interrupted In this working mode three dots will appear in Homepage instead of CO2 current value and the indicator will turn yellow When using the OKO AIR PUMP BL as Air Source the maximum flowrate value you can set is 0 4 min 9 3 7 2 Gas cycles This function allows switching between two different CO concentrations z Fay o Settings Gas Se Settings Gas o okolab SP 2 o Air Source CO2 N2 Source Flowrates time e Gas cy Status Sensor n calibratio
5. ANY NATURE OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT 5 AC section wiring diagram Figure 1 shows the alternating current AC section designed to connect internal power supplies see C in Figure 1 in parallel to low voltage power grid A Power Connector OM SP8210263 B AC DC Relay Crydom DRA1 CX240D5 C Power supplies TDK Lambda ZWS30 12 and ZWS5 5 Q o 5 3 8 2 3 9 o X AX 582 8 Q SLATO a 9 Dasm gt O lt 2 O GENIN lt z E2 3 N E m N di NA 0 Z X sss rai see seas p I i C na D 3 gt SZ z E 2 l so g 3 I 0 z i lt a i I I I A A 9 i ES gt m o I i Zo 3 S o 252 IR S Lu lt i TEPEE S i 90 Sa b S SW 4A ES Fs _ e nei 2 TRAE TS E er E POME TP o o i Z I de S 0 7 l DES zE wm ES 503 l i aa naa m o lt I i i Dv I a I I I S I J Do zk I a RS s 3 E i a ia l l co l l E JE I I E I i Ln o G i gt 0 I 4 5 gt Q 9 I Y Oo x lt Z E O3 m Youd z Y I I o 1 I I 1 Sty lt 3 I I O CO N t lt I quane N s I I A C lt lt s BD 0
6. Screen Settings are adjusted by following the two steps below look at the touching finger 10 15 AM Touch Screen Figure 15 Touch screen settings 9 3 5 Setting Date and Time The first time you turn the system on it is important to set date and time Use the and to set date time and then press Save 21 settings Touch screen x V Options Brightness Calibration Qs 2 Alarms Factory Reset Figure 16 Touch screen settings Date and Time Press Home first one on the left menu to go back to the main page 9 3 5 1 Options submenu In this window you can set the scale of the X axis time duration of the graph displayed in the main page Ce okolab Settings Touch Screen Options settings Touch Screen Chart history length 120 minute O O Brightness Calibration Updat 120 s Date amp Time Alarms Factory Reset Cancel Save 10 15 AM Figure 17 Chart Options Press and slide your finger along the setting bar or simply click to set desired value then press Save Tip gt Recommended value for the chart history length is 60 or 120 minutes 9 3 5 2 Alarms settings This page allows you to set deviation value from set point beyond which you want the system to trigger an Alarm 22 settings Touch Screen settings Touch screen Alarms a Q E A te Options Brightness Calibration Humidity o si Date amp
7. 4 vl G cul e elle OIOIOIO l l I i ele o o l I i i I j 1 eon ES A I I A I I I I l I A al 1 I 2 E 1 I I Doe glie K C P E rifai ee iero dia io gle girini tee ae A s RS ie iii A i o 2 I a co y E o 0696 E n Va ca sz O 54 lt H lt zs N LL LL zZ Hi Oo M N m 10 6 Equipment and connections 6 1 Equipment supplied and Tubing details 1 CO2 Controller main box 2 OKO TOUCH touch screen NOTE CO2 UNIT BL requires the touch screen interface OKO TOUCH to be operated OKO TOUCH is purchased separately When purchased OKO TOUCH is provided with the following items BV DC Power adapter x1 with power cord RS232 serial cable to connect it to any one of Okolab digital controller 3 TUBE A x2 Rigid tubes 3m long 6mm OD each Use TUBE A to connect the output ports of Air and Carbon Dioxide tanks to the corresponding input ports Air and CO2 on the rear panel of the CO2 Controller 4 TUBE C x1 2m long blue rigid tube 6mm OD with attached a 4mm ID silicon transparent tube 1 m long plus PTFE filter in the middle Use TUBE C to connect the CO2 Controller to your device or to the humidifier if it is present 5 Pressure Gauge for CO2 regulator assembly stirrup x1 Install pressure gauge between the pure CO tank pressure regulator and the CO2 Controller input ports PTFE filter x2 One to be installed on the rear of the U
8. Ne AAN RO V kor Nit pb na boy oli Un Co cil i 2 Uni Il a CO2 UNIT BL and H301 T UNIT BL PLUS b CO2 UNIT BL and H301 T UNIT BL PLUS Units rear panels 3D view Figure 6 CO2 UNIT BL and H301 T UNIT BL PLUS The T Unit and CO2 Controller must stack on top of each other by lining up the bus ports located on the top and bottom surface of each When the units are properly connected the Temperature and CO parameters will appear on the OKO TOUCH Touch Screen interface Home page If not all the parameters appear on OKO TOUCH Home Page then the Control Units are not properly connected Please check that the bus ports are properly aligned with each other If you have the CO2 Controller and a Temperature Control Unit it is essential to read both this manual and the Temperature Control Unit Manual to familiarize yourself with the functions and the operation of the devices before use 9 CO2 UNIT BL Installation and user guide The following paragraphs will illustrate how to install and to use the CO2 Controller 9 1 CO2 UNIT BL Installation guide The following instructions shall give you the possibility to install the unit quickly For safe operation of the unit it is absolutely necessary to read carefully all the instructions and safety notes 15 Install the PTFE filter on the rear of the CO2 UNIT BL It filters the Air CO flow coming from their tanks before entering in contact with
9. PY ee okolab bold line Figure 12 Screenshot at Startup Once the system has been initialized the led on CO2 Controller will stop blinking and will turn steadily on The Homepage will appear on the touch screen see Figure 13 The color of the light indicator on the CO tab will be initially yellow 4 Select the set point gas composition from the touch screen Home page 5 Wait until the light indicator on the Home page become green 19 9 3 Unit operation through the Touch Screen 9 3 1 Home page CO2 set point Current CO2 value Max CO2 reached within the time period roy 90 0 Touch here to know generic okolab Min CO2 reached within the time period info about CO2 Controller and running time Touch here at any time to come back to the Main page Psi Touch here to access system options and settings Touch here to go to 10 15 AM the OVERVIEW page Figure 13 Homepage of the CO2 Controller Touch Screen Display Chart history lenght set at 1h On the right side of the screen the graph chart displays the CO level over time On the CO tab the minimum and maximum value that CO has reached within the chosen time period is displayed on the bottom and top hand side corners respectively Tip gt The OKO TOUCH is pre set at a 5 CO concentration hence when you witch it on the system will start operating to reach this set point value 9 3 2 Colours meaning tolerance you ve set and that the system is w
10. arbon dioxide gas leaks continuously out of the instrument and should never be allowed to build up in room VENTILATION unit should be situated so that its location or position does not interfere with proper ventilation Neither the gas mixer nor stream destinations should be in poorly ventilated areas Unit should be situated away from heat sources such as open flames radiators heat registers stoves or other appliances or processes that produce heat Do not start up the equipment if the supply cable is damaged Connect the equipment only to grounded mains power socket Do not disconnect cables while in operation Do not open the unit Do not remove cover or back Prevent metal fragments or lead wire scraps from falling inside instrument to avoid electric shock fire or malfunction No user serviceable parts inside Before starting assemble the equipment far from electric device Unit should never be used where it can fall or be pushed into water International caution symbol marks this device It is Important to read the Safety Notes before installing using and commissioning this device as A the notes contain important information relating to safety and EMC Not following these instructions can result in damage or breakdown of the device and its accessories We reserve the right to make technical variations IN NO EVENT SHALL OKOLAB S R L BE LIABLE FOR ANY DIRECT INCIDENTAL OR CONSEQUENTIAL DAMAGES OF
11. arrow on the rear of the Pressure Gauge for the correct Gas In Out See Figure 3 Figure 3 Input and Output gas ports See symbol on the rear of Pressure Gauge On the bottom of the unit there is an automatic purging valve Close it before operating the Pressure Gauge To close the purging valve looking at the regulator vertically from the top knob pull it down and rotate it counterclockwise See A and B in Figure 4 Now you can operate the Pressure Gauge 13 wu CIS Figure 4 How to close the purging valve of the Pressure Gauge To close the gauge pull the knob up and rotate it counterclockwise Push the knob down to lock it Open valve on gas supply tank Adjust Pressure Gauge by pulling the knob up and rotating it clockwise until the pressure reaches the desired value See Figure 5 then push the knob down to lock it Figure 5 Pressure gauge using 8 How to assemble CO2 UNIT BL with a Temperature Unit The C02 UNIT BL can be used as a stand alone device or in combination with any one of the Bold Line Microscope Incubator Figure 6 shows how to assemble the CO2 UNIT BL for example with a H301 T UNIT BL PLUS 14 rv ee pe cme Ee Temperature Temperature Poveri DAS ES xL G 8 gt B Be B LE D 2 Fuse Heater Fuse2 Hester2 Fuse HM 1 Fuses HM2 Cc MOT FOR CLINICAL USE ON HUMANS MADE IN ITALY boys l
12. auge ee eeeeeeeeeeeeeeeeeeees 13 Figure 4 How to close the purging valve of the Pressure Gauge iii 14 FIGUKG 5 Pressure galge Siria 14 Figure 6 CO2 UNIT BL and H301 T UNIT BL PLUS ciii aida 15 Foure 7 Fer ise ivi ii i rici 16 Figure 8 Avoid gas leak strongly push the tube into the Push in to Fit connector cece ee 16 Figure 9 CO2 and Air Tanks connections to the back panel of the CO2 Controller ii 17 Figure 10 CO2 Tank and Air Pump connections to the back panel of the CO2 Controller Le 18 Figure 11 Oko Touch Connection i 18 Foure 12 Screenshot al SlanUDs suse stadia blass 19 Figure 13 Homepage of the CO2 Controller Touch Screen Display iii 20 Figure 14 How to change the COs Sel PON eisene anipe E AEE EEE 21 Figure T5 FOUCA SCIEENSEMINOS isalaziniala nella lei 21 Figure 16 Touch screen settings Date and Time 0000000 rr 22 Foue 1 CMa OPUN aseisiin pn ar inan rA E eeteta rotores EAEE 22 Figure 16 HOw T0 enter AAS PAYS 23 Figure 19 How to set the Deviation and Time Alarm lt lt lt 000000000 K K K K K K 23 HOLE 2015 SUING estr distales calida 24 FOULEZ Gas SEMO SUD corali iaia A O E CR BE CE A 24 FigbUre 22 Air SOUCe Dade PEP E de inodoros 24 Figure 29 MIOW ales VIEW issue iaa aos 25 Figure 24 COs O Ae 25 Figure 25 COs Concentrations Cycles SEHINgs ian 26 Figure 26 Cycle Summary an
13. d Configuration Saving ii 26 AAA Cdl E di aa n e eo ale cele 27 Figure 28 Icon in Homepage during a CO2 Cycle 27 Figure 29 Warning shown if you try to change setpoint during a gas CYyCle i 27 Figure 30 How to reach the Sensor Calibration SUDMENU nr 28 Figure 1 Sensor Calibration SUBMENU sets snad Rn 28 Figure 32 External Gas Meter Connection iii 29 Figure 33 Manual Calibrationi cn A A 29 Figure 34 Manual Callbration Correcto sarei i a 30 Foure 99 Manual Calibration ENG sini 30 Figure 36 How to insert the Span Gas Calibration parameters iii 31 FOPO OBA SAD AOR Staind csds 31 ao aso DAN ODO DOC O C zuzana season aids 32 Figure 39 Homepage during the Span Calibration lt lt lt lt lt lt nnn nK K EEE KKK 33 Foure 40 Status Page vst ear O na 33 38 WARRANTY Okolab S r l warrants its products to be free of defects in materials and workmanship for a period of one year starting from invoice date If the unit malfunctions it must be returned to the factory for evaluation If the equipment has to be returned to the factory please ensure that is carefully and properly packed Okolab S r l accepts no responsibility for damage due to unsatisfactory packing Upon examination of Okolab S r l if the unit is found to be defective it will be repaired or replaced at no charge This warranty does not apply to defects resulting from a
14. eason you want to reset to the factory calibration simply go to the Sensor Calibration page Figure 32 and press Factory Reset 9 3 9 Status Page This page displays the Status of the CO2 UNIT BL This is useful to check that the system is working properly Most of the parameters displayed are technical control parameters that you may be asked access in case you have requested for Okolab support settings Gas Status Valve opening Flow Gas gt Duty mA p limin o Air 64 6 735 34 5 0 380 co 29 0 554 19 7 0 020 50 790 Total 0 400 Pump 100 123 Figure 40 Status page The values that may be of interest to users are Flow and Gas All other parameters are just to be communicated to our technicians in case you request Okolab support 33 10 Maintence To get a correct Gas controller operation over time m Usea polishing cloth or dry cloth to wipe off dust and dirt A Before cleaning the unit unplug it from the power source Water must not enter the system Never use thinners benzene solvents on or near the devices since these could corrode the surfaces Verify the status of all hoses tubing and replace those damaged After 2 years disconnect all polyurethane tubing cut the last 1 cm of the tubing and reconnect them to their ports 34 11 1 Web conference for assistance and training In case you requested online support and or web conference assistance for system assemb
15. erence After pressing Adj CO2 an offset resulting from the calibration will be displayed on the Offset tab last tab on the right Now you can press the Home icon to go back to the Main page ee Settings Gas Offset okolab External Probe CO Unit Offset gt gt a 5 0 0 2 Total Flow 0 400 jmin Figure 35 Manual Calibration End Please make sure that the external Gas meter you use has been recently calibrated AN and that it has sufficient accuracy It often happens that external Gas meters are less accurate than our CO2 UNIT BL and are unisuitable to perform the calibration described above Finally if you are unsure on the calibration you have just performed or if for any reason you want to reset to the factory calibration simply go to the Sensor Calibration page Figure 31 and press Factory Reset 9 3 8 2 Span Calibration OPTIONAL This function allows performing a calibration through a Span Gas Tank Before starting the instrument calibration make sure you have a Span Gas Tank connected to the port labeled 30 Span Gas 2 Input on the rear of the CO2 UNIT BL then press Span Calibration In the subpage that will open click on the Span Gas Image green tank to start the calibration procedure By flagging the option Run at start you choose to automatically run the Span Gas calibration every time you turn the system on Make sure that a Span Gas tank remai
16. inder Figure 28 Trying to change set points or starting a new cycle while a different one is still running will open the window in Figure 29 You can press Abort Cycle to modify it or start a new one Pressing View will open the current cycle settings Figure 29 Warning shown if you try to change setpoint during a gas cycle 9 3 8 Sensor Calibration This function allows performing the calibration of the instrument 27 Mi Air Source Op N Source Fiowrates f _ Gas cycles Status calibrati Figure 30 How to reach the Sensor Calibration Submenu 9 3 8 1 Offset Here you can calibrate the instrument by using an external CO gt 0 meter NOT hereby provided settings Gas sensor Calibration Y Span Calibration Factory Reset An external probe Calibrated gas isneeded sources are needed Figure 31 Sensor Calibration Submenu To run the gas sensors calibration through an external gas meter press Offset It is necessary to connect the external gas meter to the port Gas Output on the rear of the CO2 UNIT BL see Figure 32 First disconnect the blue tubing inserted into the Gas Output port Remove it by keeping the black ring of this connector pushed in you should feel no resistance in pulling this tubing out If you do make sure you are properly pushing in the black ring 28 CO2 UNIT BL External Gas Meter Tubing not supplied
17. ling and experiment support you received a web cam Please follow these guidelines to receive optimum service Webcam installation instruction and Cdrom included Last Skype software installed www skype com Register yourself on www skype com to have an account Skype_ID Set the audio and video and test them using Skype Contact our technical support sibillo oko lab com by e mail to take an appointment for the web assistance 11 2 Troubleshooting Incorrect operations are often mistaken for trouble and malfunctions If you think that there is something wrong with a component check the points below Sometime the trouble may lie in another component Investigate the others components electrical appliance and pneumatic system being used If the trouble cannot be rectified even after exercising the checks listed below ask for Okolab support at info oko lab com Symptom Probable cause Remedy Power plug is disconnected trom Insert plug securely into outlet The little led on the front of the Control Unit outlet is off Replace the fuse 1A Time Lag Blown fuse Fuses Check reservoirs and or compressed No CO or Air flow rate stability Pressure drop line Check Air CO tanks make Sure they re not close to finish No CO and or Air flow streams Regulators closed Open regulators Make sure you ve attached the PTFE No CO Air arrives to the incubating o E filter on the back of the CO2 UNIT Missing connection chamber BL Co
18. lternative solution for Air supply is the OKO AIR PUMP BL Illustration of the Main Units 7 1 CO2 O2 Unit BL 0 10 0 1 Air Input CO2 Input a MADE IN Figure 2 Gas Controller Rear Panel Overview Power Input OKO AIR PUMP BL connector Leave unplugged if the system is not equipped with OKO AIR PUMP BL OKO TOUCH RS232 serial connector USB connector Heated tube connector To connect the heated tube to the CO2 Controller only if you have ordered HM ACTIVE Gas Output 12 10 11 12 13 14 15 Filter Out To connect the PTFE filter Humidifier Connector To connect the Humidifier Heated Base to the Control Unit only if you have ordered HM ACTIVE Humidity Sensor Lid with Temperature control Connector To connect the Sensing Cell to the CO2 Controller only if you have ordered HM ACTIVE Filter In To connect the PTFE filter Air Push to fit Input Connector for 6 mm O D rigid tubing carrying air from air source 5 pole bus connector used to connect the Unit to another Okolab Unit for example a Temperature Unit Pure CO2 Push to fit Input Connector for 6 mm O D rigid tubing Span Gas Push to fit Input Connector for 6 mm O D rigid tubing use only when calibrating the unit 24VDC Input Power plug Used only if the HM ACTIVE is present 7 2 Pressure Gauge set up and use Pressure Gauge for the CO2 line to be installed between the CO2 tank and the CO2 UNIT BL Follow the
19. n 10 15 AM E Del hoz Figure 24 CO Cycles Subpage 25 You can decide whether setting a new thermal cycle New or recalling a previously saved one Load To set a new CO concentration cycle first choose the two CO concentrations between which you want to switch SP1 Set Point 1 and SP2 Set Point 2 Next set the duration in hours and minutes you want the system to dwell on each concentration Lastly set the number of cycles Number you want the system to perform see Figure 25 Settings i Gas New Settings Gas New COs semi perlod time hhimm Number Figure 25 CO Concentrations cycles settings Once you have adjusted the settings a Cycle Summary page will be displayed Press Save As if you want to save the CO Cycle program to be recalled for future experiments You can save up to 4 different CO cycles programs Saving a 5 one will overwrite the first saved one Ga settings Gas Cycle summary settings i Load saved Cycle LO summary summary Pr L evo oe Cake 1 Configuration 2 Configuration 3 Configuration 4 Figure 26 Cycle Summary and Configuration Saving In the last step you can schedule the time you want the thermal cycle to start 26 A AAA coem er Cycle Stel ime Figure 27 Cycle Start At set time the cycle will start A concentration cycling icon will be displayed on the Homepage as a rem
20. nit and one spare replacement RS232 Serial cable x1 To connect the touch screen interface OKO TOUCH to the CO2 UNIT BL 8 Power cord x1 To power CO2 UNIT BL The CO2 UNIT BL can be powered at a voltage within the range 115 230VAC It can be equipped with e OKO AIR PUMP BL optional that includes Grey 3 pin power cable Use the grey 3 pin power cable to connect the Air pump to the CO2 Controller TUBE B 2 m long tube composed of 6 mm OD rigid tube 0 5 m long connected to 4 mm ID silicon transparent tube 0 5 m long then connected to an hydrophobic filter 0 2 micron porous connected to 4mm ID silicon transparent tube 0 5 m long and then connected to 6 mm OD rigid tube 0 5 m long Use the TUBE B to connect the OKO AIR PUMP BL to the port labeled Air In on the rear panel of the CO2 Controller e HM ACTIVE optional that includes Humidity Sensor Lid with Temperature control Humidity bottle and its Humidifier Heated Base Heated and Insulated Tube 11 24V DC Power supplier feeder x1 e SM BL optional that includes Webcam x1 USB cable x1 6 2 Equipment NOT supplied CO tank with safety valve pressure gauge regulator to accept rigid tubing 6 mm OD Gas source must be high purity medical grade and humidity free gas Air tank or compressed air line with safety valve pressure gauge regulator degree of filtration 20 um and condensate drain to accept rigid tubing 6 mm OD NoteP An a
21. nnectors 8 and 11 in paragraph 7 1 l The current CO value is far from l l Acoustic alarm sounds l Check the CO and Air supplies CO set point The little led on the front of the Control Unit Check the connection between Serial cable is disconnected is green but the Oko Touch is off Control Unit and the Oko Touch Strongly push the tube into the Gas leak on the rear of the control box Not properly inserted tubes o Swift Fit connector I checked the previous troubleshooting but 1 Contact Okolab to receive cannot solve the problem assistance 35 11 3 Technical support Please do not hesitate to contact Okolab should you need any further commercial information or technical support Please check Okolab web site www oko lab com for news events new products and general FAQ For COMMERCIAL SUPPORT lanzaro oko lab com Phone 39 081 806 2624 Fax 39 081 876 4410 Mobile 39 348 9680717 For TECHNICAL SUPPORT sibillo oko lab com Phone 39 081 806 3470 Mobile 39 348 9680718 Okolab S r l Via A Olivetti 1 80078 Pozzuoli NA Italy 11 4 Italian Technical Specifications CO2 UNIT BL Technical Specifications Range 0 18 CO Step size 0 1 Accuracy 0 1 5 0 Recommended Calibration Interval 1 year N Figure 1 AC section wiring diarrea cri ed dera 10 Figure 2 G46 Controller hear Pane POVEervieW isessrcsni iaia ein 12 Figure 3 Input and Output gas ports See symbol on the rear of Pressure G
22. ns connected to the system at all times UE Ge NE Fedor a settings Gas Sensor Calibration span I Factory Reset Offset Span amp An external probe Calibrated gas is needed sources are needed Di ea a fa 10 15 AM YD gt Figure 36 How to insert the Span Gas Calibration parameters Input the Concentration and Deviation specifications of your Span Gas tank as reported on the calibration document you received with the Span Gas tank Then press Save and Start to run the Span Gas Calibration Procedure settings Gas Sensor Calibration span Settings Gas pan Gas 1 3 m Run at start Cancel Figure 37 Span Calibration Start The calibration procedure goes through different steps from zero to max values and it takes about 30 minutes to complete 31 i calibration Figure 38 Span calibration procedure You can press Abort if you want to stop and cancel the calibration procedure or Home to return to the Homepage while the calibration procedure is still running On the Homepage on the CO tab you ll see a symbol indicating that the gas sensor calibration is running in the background See Figure 39 32 raw ce okolab Sensor calibration in progress Icon 10 15 AM Figure 39 Homepage during the Span Calibration Finally if you are unsure about the calibration you have just performed or if for any r
23. ns can result in damage or breakdown of the device and its accessories The products labels can be found on the bottom panel of the Main Unit Use bi distilled water for gas stream humidification if HM ACTIVE is present Do NOT go in close contact with or breathe any gas stream whose composition is different from that of ambient air Do not exceed voltage indicated in this manual and on the product label Avoid excessive induction noise static electricity magnetic fields Do not expose this instrument to water or moisture Prevent throttling and kinking of tubing Check tubing time to time for possible material usage Check that all tubing is well inserted into the connectors so they cannot slip off This device is not designed for use for medical applications Install safety valves and adequate pressure regulators on gas lines before the CO2 Controller input connectors Power cord of unit should be unplugged from electrical outlet when left unused for a long period of time PRESSURIZED GAS secure all connections with hose clamps Never exceed pressure limits 1 5 Bar Bleed all lines before disconnecting Wear safety goggles if needed If pressure regulators are not within sight and reach make sure at least one shut off valve within reach LOW OXYGEN ATMOSPHERES never enter a room or enclosure which has a low oxygen atmosphere because of severe danger of suffocation Only operate in well ventilated room A small amount for c
24. ny actions of the purchaser Okolab S r l neither assumes responsibility for any omissions or errors nor assumes liability for any damages that result from any action of the purchaser that discord from instructions listed in the operation manual This warranty does not cover or involve any other equipment that may be used along with the Okolab System i e mini incubators any gas tank etc whose usage should be considered independent and performed according to their own operational instructions Okolab S r l warrants only the parts manufactured by it will as specified and free of defects Okolab S r l makes no other warranties or representations of any kind whatsoever express or implied except that of title and all implied warranties including any warranty of merchantability and fitness for a particular purpose are hereby disclaimed LIMITATION OF LIABILITY the total liability of Okolab S r l shall not exceed the purchase price of the component upon which liability is based In NO event shall Okolab S r l be liable for consequential incidental or special damages 39
25. ock CAUTION or WARNING or IMPORTANT This symbol warns you of circumstances or practices that can affect the instrument s functionality Please refer to accompanying documents Tip gt Supplies you with helpful suggestions Note gt Supplies you with important information to successfully setup and use the instrument 3 1 2 Symbols on the product label CE MARKING This symbol indicates a product s compliance with EU legislation Y PRODUCT DISPOSAL This symbol indicates that this product must not be disposed as urban solid waste pe IP 30 This symbol indicates the protection degree against ingress of solids or liquids inside the product 4 Safety Notes Before operating the equipment please read carefully the instructions and the safety notes If you have any questions please contact Okolab The equipment must only be used as intended and as described in this Manual Equipment should only be operated by technically qualified personnel Do not start up the equipment if some of its parts are damaged This instrument is not intended for use in locations subject to flammable or explosive gases Transport the equipment with care Equipment and its internal parts can be damaged by dropping and by shock Do not use a volatile solvent such as paint thinner to clean the instrument Deformation or discoloration will occur Use a soft dry cloth to remove stains from the instrument Not following these instructio
26. olour Medda a TE AE e ea 20 9 3 3 Changdng Set Ol REMO R E iaia nt 21 934 e SS PPP a E a radiali 21 9 3 5 Senna BAe MS tesco 21 9 010 Gas DO UIMOS serra rola ooo OI 23 or iS A O ECO E o II 24 Ioe S OP A O O E E E E 27 9 3 9 SAU Pe edn Aedes io 33 10 MAINT ENC E siiin EE S RA SERU IRASA AA k 08 34 11 Ue T 35 11 1 Web conference for assistance and WaiNiNg vivieron e 35 1122 TOUDISSNOOHAO pia 35 ES Tecnica SUD DOME sc tnatthetsqae adi tamerine rama aria 36 114 Halan Technica SoeCiiCanOnS intasare 37 12 FOURE ee ee ee ee re 38 1 Revision table cited December 2014 Amelia Lccardo 2 Silvia Foppiano 0 Revision March 2015 Amelia Liccardo CO2 UNIT BL is a Digital CO2 Controller that generates CO Air mixtures by mixing together Air and CO2 The CO2 Controller is designed to be used along with microscope incubators or perfusion chambers for Live Cell Time Lapse Microscopy and for all the applications where a gas mixture of CO in Air is requested It allows controlling CO concentration in the range 0 18 with an accuracy of 0 1 It can be used as a stand alone device as well as in combination with any kind of microscope incubator Both Air and CO flows and levels are digitally controlled A CO infrared sensor continuously measures CO concentration in the mixed gas stream and a PID closed loop controller gives feedback to a fine valve regulating CO flow CO2 UNIT BL requires a touch screen interface OKO TOUCH to be ope
27. orking properly points NO actions on you part are required Please note the Yellow light will also A GREEN indicator means that set point value has been reached within the A YELLOW indicator means that the system is working towards reaching set appear every time you change CO set point its value is out of the set tolerance see section ALARMS in paragraph 9 3 5 2 Most An ORANGE indicator means that the current gas concentration is not correct and commonly this is related to gas leaks or gas source s running low Verify that all cables are correctly connected Check all tubing for gas leaks and pressure in gas supply tanks Controller If after few hours the Red light doesn t turn to Green turn off the system The RED indicator means that something may have been damaged inside the CO2 20 and then turn it on again Turn the system off wait 5 minutes and turn it back on If the problem persists contact Okolab at http www oko lab 9 3 3 Changing Set Points To change set point simply touch the tab for CO the setting panel will appear on its right Simply press on and to set the CO you desire then press Set to save the new set point or Cancel to undo S OTT E RAEE TE Oe EO A TA A T A EAS TE TAI w 1 4 f W JA 1 dia gt y AE hu l A it ec o nes n da 09 15 AM 10 13 AM de e At ie i e Figure 14 How to change the CO set point 9 3 4 Touch Screen Settings Touch
28. rated Purchased separately Acoustical alarms can be selected and enabled disabled by the end user The alarm alerts the end user if deviations from set points of specific magnitude and direction occur The CO2 UNIT BL digital controller can be used with stage top incubation systems and equipped with HM ACTIVE optional a system that controls the Relative Humidity inside the micro environmental chamber It consists of three parts a Humidity Sensor an Heated and Insulated Tube and an Humidifier Module heating the water container When C02 UNIT BL is used with a Cage Incubation system it can be equipped with a vibration free humidity module HM VF which includes a water trapping system preventing water from ever reaching the sample If the laboratory does not have an air source available e g 100 Air tanks or compressed Air lines then an air pump OKO AIR PUMP BL can be requested and directly connected to C02 UNIT BL OKO AIR PUMP BL is a plug and play solution for Air inlet and a convenient alternative to 100 Air tanks compressed Air lines Please read this manual to familiarize yourself with the functions and the operation of the device before use Make sure to read the HM ACTIVE User Manual if HM ACTIVE is present in your system 3 Symbols descriptions 3 1 1 Symbols in this manual and on the product The following symbols identify important information to note CAUTION or WARNING This symbol warns you about the risk of electrical sh
29. sensing cells It prevents sample contamination from bacteria and gas sensors from damages caused by dirty gas streams It is connected with two 4 I D mm silicon tubes with the Filter In and Filter Out connectors on the Rear of the CO2 UNIT BL Figure 7 Filter tubing Tip gt Replace this filter each 2 3 months of continuous use Do not remove the filters This may cause damages to the gas sensor and sample AN contaminations Tubing for CO and Air lines are rigid tubing 6 mm O D TUBE A in par 6 1 and they connect to the CO2 UNIT BL through Push in connectors Make sure to push tubes securely all the way into connectors avoiding any gas leak To remove tubing from push in connectors push the black or red rubber ring while pulling the tubing If tubing doesn t easily come out do not force it simply make sure the black ring is properly pushed Figure 8 Avoid gas leak strongly push the tube into the Push in to Fit connector 16 Before disconnecting any of the polyurethane tubing connected to tanks or to the CO2 Controller make sure there is no residual pressure by adjusting the pressure gauges upstream of the system and the tank manometer 3 Figure 9 shows the connections between the CO and Air tanks and the respective connectors on the rear panel of the CO2 UNIT BL NOT FOR CLINICAL USE ON HUMANS TUBE A Paragraph 6 1 T
30. ubing connection not supplied Air Figure 9 CO and Air Tanks connections to the back panel of the CO2 Controller Controller Tip gt If you have ordered the OKO AIR PUMP BL you have to connect TUBE B see par 6 1 between the Air Pump and the connector labeled Air Input on the rear panel of the CO2 17 CO2 Input TUBE A Paragraph 6 1 CO TUBE B Paragraph 6 1 Tubing connection not supplied Figure 10 CO Tank and Air Pump connections to the back panel of the CO2 Controller 4 Connect the touch screen interface OKO TOUCH to the CO2 Controller with the RS232 serial cable as illustrated in Figure 11 Connect the 5V DC Power adapter to the OKO TOUCH CO2 UNIT BL UA l Il ul 5VDC Power Feeder Electrical connection Points 7 and 9 in par 6 1 Figure 11 Oko Touch Connection 5 Connect the power cord to the CO2 Controller 18 9 2 CO2 UNIT BL Set up the system and Turn The Unit On Brief instruction to set up and turn on the unit 1 Regulate the pressure of each gas before the CO2 Controller at 1 0 barg 14 4 PSI Do not exceed 1 barg Check that all the cables are properly installed as described in the previous paragraph 3 To switch the CO2 Controller on use the rocking switch on the side of OKO TOUCH The led on the front of the CO2 Controller will turn green and the screenshot in Figure 12 below will briefly appear on the Touch Screen

CO2 UNIT BL - User Manual

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