BioPette Plus Multichannel User Manual 2010
Contents
1. Model Volume Accuracy Precision Fit to tips ul ul Min 1 8 0 6 0 BPPT 10 5 4 0 2 0 10 Max 10 2 0 1 2 Min 5 4 0 2 5 BPPT 50 25 3 0 1 2 200 Max 50 1 6 0 6 Min 20 3 0 1 5 BPPT 200 100 5 0 8 200 Max 200 1 0 0 6 Min 50 1 6 1 5 BPPT 300 150 1 2 1 0 300 Max 300 1 0 0 6 These specifications are obtained in forward mode using a gravimetric method with the temperature of the distilled water tips and all other conditions stabilized between 19 C and 21 C The values given include all components of error due to both normal hand warming and changing of the tips Performance tests The pipette is calibrated in accor dance with EN ISO 8655 Performance can be verified by checking the pipette using the procedures outlined in the EN ISO 8655 standard _ BioPette MULTICHANNEL The pipette design enables the user to perform the recali bration process according to the rules presented in sec tion 5 2 SETTING THE VOLUME The setting of the volume of the aspirated liguid can be done either by the pipetting pushbutton knob Fig 1A2 or by the adjustment knob Fig 1B The volume adjust ment can be performed when the locking ring is set in the lower position Fig 5A When the desired volume is selected the locking ring should be set in the upper position Fig 5B The volume shown by the counter is represented by three digits which should be2. _ BioPette MULTICHANNEL Labnet International Inc PO Box 841 Woodbridge NJ 07095 USA Phone 1 732 417 0700 Fax 1 732 417 1750 email labnet labnetlink com www labnetlink com BioPette MULTICHANNEL BioPette MULTICHANNEL _ BioPette MULTICHANNEL CONTENTS 1 INTRODUCTION 2 VOLUME SETTING 3 METHOD OF PIPETTING 4 RECOMMENDATIONS 5 RECALIBRATION 6 CLEANING AND STERILIZATION 7 PIPETTE KIT AND ACCESSORIES 8 SPARE PARTS 1 INTRODUCTION The BIOPETTE PLUS series of adjustable multichannel pipettes has been designed for the filling of laboratory microplates The pipettes enable precise and simultaneous delivery of 8 or 12 preset volume doses of liquid BIOPETTE PLUS pipettes are produced in four ranges of volumes 1 10 ul 5 50 ul 20 200 ul 50 300 ul The BIOPETTE PLUS pipettes are equipped with a digi tal counter which shows the pipetting volume The set volume is visible in the window on the handle The setting of the volume is done by turning of the pipet ting pushbutton knob Fig 1A2 or by turning the adjust ment knob Fig 1B in the correct direction The pipette d
3. After waiting second the pipetting button should be pressed to the second resistance point in order to expell remaining liquid Fig 3D Then tips should be lifted out from the vessels while maintaining contact between the ends of the tips and the inner walls of the vessel until the pipetting button has been relased Fig SE Finally pressing the ejector button the opera tor should separate the tips from multichannel module s cones Fig 3F BioPette MULTICHANNEL 4 RECOMMENDATIONS To achieve maximum safety precision and reliability the following principles should be observed do not draw liquids without tips fitted on the pipette cones do not lay down the pipette with tips filled do not draw volumes of liquid exceeding the pipette s range check if the tips are fitted properly during operation the pipette must be held vertically tips should be immersed in liquid to the depth of 2 to 4 milimeters and the pipetting button should be depressed and released slowly and evenly new tips must be rewetled prior to pipetting by draw ing and expelling the liquid to be measured This is especially important when working with liquids of visco sities and densities different from that of water and in temperatures different from ambient temperature when pipeting liquids which tend to wet walls of tips Such as serums proteins or organic solvents measuring must be performed much slower then
4. be carried out The recalibration can be performed within one full turn of the key to the right or to the left only Recalibration conditions Ambient temperature and the temperature of the pi pette tips and liquid should be within the range 20 25 C and stabilized during weighing within 0 5 C Measurements should be conducted using distilled water Balance sensitivity should be suitable for the volume to be controlled Recalibration procedure Set the dose volume depending on the pipette volume according to the following table Range Volume change ofthe Permissible AV for full turn of pipette volumes the calibration volumes ul key ul ul 24 increments BPPE 10 BPPT 10 0 92 1 08 0 33 BPPE 50 BPPT 50 BPPE 200 BPPT 200 BPPE 300 BPPT 300 4 8 5 2 19 6 20 4 49 2 50 8 Perform three aspiration series each series should include the aspirations from all channels weigh each time and calculate the average value of the aspirations BioPette MULTICHANNEL Calculate average aspirated volume in ul multiplying the average aspiration amount mg by the distilled water density coefficient ul mg which depends on temperature and pressure according to the following table Pressure kPa Temperature C 95 0 101 3 105 0 If the average aspirated volume exceeds the permissible value the following should be done
5. drying cycle prior to sterilization unscrew the nut connecting the handle and module slightly After autoclaving these parts should be screwed tight again to set the locking ring in lower unlocked position prior to sterilization The precision of the results should not alter if the pipetting process and autoclaving are carried out as described in this manual Because a slight change in the accuracy of the dosage may occur it is recommended to check the calibration of the pipette after the initial first third and fifth autoclaving cycles and then after every 10 autoclaving cycles Pipette kit The pipettes are delivered in the kits including Pipette Instruction manual Calibration key Shelf clip dentification labels The shelf clip assembly diagram is shown in Fig 6 Accessories Model Tip Choices BPPE 10 BPPT 10 P1000 10 R 10 ul tips racked BPPE 50 BPPE 200 BPPT 50 BPPT 200 P1000 200 R 200 I tips racked BPPE 300 BPPT 300 P1000 300 R 300 ul tips racked Pipettor Stand Choices BPPE BPPT P3989 Universal linear stand BioPette MULTICHANNEL 8 SPARE PARTS The spare parts for multichannel in Fig 1 4 5 Pipetting pushbutton A1 Pushbutton A2 Knob Adjustment knob Ejector button Piston s assembly Tip cone Ejector Calibration key Locking ring lt o m m O O D gt can be ordered from your Labnet representative t
6. set the locking ring into the upper position thus locking the knob and preventing accidental volume change 3 METHOD OF PIPETTING Fit the tips onto cones of the multichannel module In order to put tips on the multichannel cones hold the pipette vertically and press it against the tips in the rack box until the cones retreat about 1 5 mm into the multi channel module The suspension system of the cones ensures even and tight sealing of tips The pipette does not need to be rolled back and forth to seal the tips tightly The liquid aspirated into the tips should not flow out by gravity from properly fixed tips While holding the pipette in a vertical position tips should be immersed in the liquid to a depth of 2 4 mm and flushed once by drawing a dose of liquid and dispensing it out with slow and steady movement Next still holding the pipette ver tically the operator should press the pipetting button until the first resistance point is felt and immerse the tips in the liquid to the depth of 2 4 mm Fig 3B Releasing the pipetting button with a slow and uniform movement during 2 3 seconds the liquid should be drawn into the tips Fig 3B which should then be lifted above the lig uid s surface The pipette should next be positioned at an angle of 10 45 degrees in relation to inner walls of desti nation vessels and tips should be emptied by pressing the pipetting button slowly until the first resistance point is felt Fig 3C
7. Remove the pipetting pushbutton Fig 4A Warning The pipetting pushbutton consists of 2 parts the knob Fig 1A2 and the pushbutton Fig 1A1 After removal of the pushbutton both parts are separated Holding the volume adjustment knob to protect it against rotation insert the calibration key into the cuts of the calibration screw Fig 4B Turn the key clockwise to reduce the aspirated volume or counter clockwise to increase the volume One full turn of the calibration key changes the pipette aspira tion volume by the amount given in the table Fig 4C Take out the key and fix the pipetting pushbutton Fig 4D The pipetting pushbutton should be fixed by placing the knob on the arbor first Fig 1A2 and then the pushbutton Fig 141 Determine the average aspirated volume The average volume should be within the permissible range given in the table If the volume exceeds the values stated the recalibration procedure should be repeated 6 CLEANING AND STERILIZATION Cleaning External surfaces of the pipetting pushbutton the ejector button the handgrip shaft and the adjustment knob may be cleaned using a cloth dampened in isopropyl alcohol Sterilization The pipette can be sterilized in the autoclave at 121 C for 20 minutes After sterilization the pipette should be dried and cooled to room temperature It is recommended to sterilize the pipette in autoclave with an initial vacuum and
8. esign allows the user to lock the volume setting by pushing the locking ring upwards Fig 1J The position of the ring is indicated by the symbols located on the handle The volume range of the aspired liquid is shown on the pipetting pushbutton Fig 1A1 BIOPETTE PLUS pipettes should be used with poly propylene disposable tips to ensure safety and accuracy Fig 1H The cones without O rings ensure the compati bility of the pipettes with a broad assortment of tips In order to protect the user against inadvertent contact with used tips the pipettes are equipped with tip ejectors Fig 1F The special ejector shape reduces the force required for their ejection The pipette construction allows to rotate the multichannel module and to eject the tips through 360 therefore the most convenient posi tion may be selected to fill the microplates It is recom mended to turn the module clockwise Fig 2 1 The specifications for accuracy and precision given in the following table are obtained using Labnet tips These figures are only guaranteed when Labnet tips are used BIOPETTE PLUS 8 channel SPECIFICATIONS Volume Accuracy Precision Fit to tips ll 26 Min 1 6 0 BPPE 10 5 4 2 0 Max 10 2 2 5 4 2 5 BPPE 50 25 3 1 2 50 1 0 6 20 A 1 5 BPPE 200 100 0 8 200 i 0 6 50 1 5 BPPE 300 3 1 0 0 6 Model BIOPETTE PLUS 12 channel SPECIFICATIONS
9. read from top to bottom Typical meter readings are shown in the following table Counter Set Basic Medel readings volume degree BPPE 10 0 3 3 5 ul 0 02 ul BPPT 10 5 Miles i 6 5 ul 0 10 ul BPPT 50 TNT BPPE 200 0 8 85 0 pl 0 20 ul BPPT 200 5 BPPE 300 2 5 250 0 ul 1 0 ul BPPT 300 J To attain the maximum accuracy set volume must be approached from a higher value by diminishing counter readings f the desired volume is lower than set volume shown by the counter the operator should turn the pipetting pushbutton Fig 1A2 or the adjustment knob Fig 1B to the direction diminishing counter readings to the required volume Before achieving the required volume slowly rotate the knob and observe carefully diminish ing reading to avoid accidentally passing the setting value If the desired volume is higher than set volume shown by the counter the operator should turn the pipetting push button Fig 1A2 or the adjustment knob Fig 1B increas ing the value until the lower figure wheel comes 1 3 of a turn beyond the required setting and then slowly backward until the setting reaches the desired volume Make sure not to pass the setting value If the knob is accidentally turned too far the process must be repeated The desired volume must always be set from the higher value in the order of decreasing value Following volume adjustment
10. with other liquids tips must be replaced with new ones when changing from one liquid to another or if drops of liquid remain inside the tips after work the pipette should be stored vertically in a holder with tips removed 5 RECALIBRATION BIOPETTE PLUS pipettes are calibrated by gravimetric method using Labnet tips and distilled water at the tem perature 20 1 C according to EN ISO 8655 standard If during pipette operation you find that the accuracy error the difference between the real aspirated volume and the preset volume exceeds the permissible value given in the table in section 1 the pipette recalibration procedure should be carried out Before starting the recalibration it is necessary to check whether the following requirements have been fulfilled during error determination the ambient temperature and the temperature of the pipette tips and water was identical the density of the liquid used is close to that of distilled water a balance with appropriate sensitivity has been used Volume checked ul 0 1 10 lt 0 001 Balance sensitivity mg 10 100 0 01 100 s 0 1 mg ul conversion factor has been taken into account the requirements given in sections 3 and 4 have been fulfilled If the above conditions are satisfied and the accuracy error for selected volume given in section 1 exceeds the permissible value the pipette recalibration procedure should
11. ype of pipette and name of the part for this pipette should be specified Warning The replacement of the piston requires con ducting of calibration procedure according to section 5 Before returning a pipette to a Labnet representative please ensure that the pipette is completely free of any contamination chemical microbial or radioactive Contact Labnet at 1 732 417 0700 fax 1 732 417 1750 email labnet labnetlink com All rights reserved Product described in this manual is subject to availa bility and technical modification Errors excepted PZ HTL S A reserves the right to improve enhance or otherwise modify its products without prior notification 2010 PZ HTL S A 9 BPPM 04 2010
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