Pulse300 Xenon Flashlamp System
Contents
1. This would prevent the correct timing operation of the flashlamp Usegimaa ustable eile en Seleleamin Higk Woltage Powells Supply Hanecessary Pulse300 Xenon Flashlamp System Operation Manual Page 8 Lamp Operation amp Position Adjustment In order to complete the setting up of your lamp with your microscope you will need to operate the lamp This will allow you to set the exact position of the lamp relative to the microscope optics and optimise the illumination field To operate the lamp you should connect the Lamp Trigger Input to a 300Hz three hundred Hertz TTL 0 5Volts frequency source This could be one of the Imagex lamp outputs or a dedicated frequency generator but please confirm that the frequency is set correctly to 300Hz before proceeding Use a BNC BNC cable to connect the lamp to the frequency source Plug one end of the BNC cable into the Lamp Trigger Input and the other end into the frequency source High Voltage Power Supply MARCO Power Minput SS e amp Trigger AA put 0 5Volts lashlamp earth Connector Head Now take the plug from the supplied DC power supplied and insert it into the DC Power Input on the High voltage power supply Do not connect the DC power supply to the mains electrical supply at this point The lamp position can be adjusted in three dimensions X left right Y up down and Z backwards forwards The backwards and forward positioning is performed by looseni2. by a 24volt switched mode power supply You should not use any other power supply unless you have consulted with Photonic Research Systems first Any attempt to use a power supply other than the one supplied with the system will invalidate the warranty The Pulse300 is supplied in 4 parts A The Mounting Plate This is used to connect the system to a Nikon microscope A Mounting Plate B The Extension Tube This attaches to the mounting plates and accepts the flashlamp housing It allows the distance of the lamp from the microscope to be adjusted by the user B Extension Tube Pulse300 Xenon Flashlamp System Operation Manual Page 2 C The Lamp Housing amp High Voltage Supply This component consists of two parts connected by a length of flexible conduit or pipe The sections should never be separated except by a qualified electrician High voltages are present inside the power supply flashlamp head and conduit Please note that the Xenon flash lamp head is attached to the high voltage power supply unit by a length of flexible conduit The cables in the conduit carry high voltage pulses and therefore you must not attempt to remove the head from this conduit The length of the cables in the conduit determines the pulse properties of the lamp Any attempt to change the length of this cable may result in a deterioration of performance pulse length and stability and expose the user to high voltage hazards D DC power supply
3. together 2nd step You should now have the extension tube attached to your microscope The flashlamp head can now be slid into the extension tube Pulse300 Xenon Flashlamp System Operation Manual Page 6 Note If you cannot access both ends of the corner screws that attach the extension tube to the mounting plate it may be necessary to hold the end of the screws while tightening the nuts with a spanner Take care not to damage the thread of the screws whilst doing this 3 Attach the Flashlamp head C Once you have successfully mounted the extension tube you may now slide the flashlamp head into the extension tube The extension tube has three screws which allow you to lock the position of the flashlamp head in place Do not overtighten these screws ighten extensior lock lamp sition Pulse300 Xenon Flashlamp System Operation Manual Page 7 4 Position the High Voltage Power supply correctly Once you have mounted the flashlamp head you must consider the position of the High Voltage Power Supply that is attached to it Please ensure that the conduit that connects the flashlamp head to the High Voltage power supply is not put under stress If the microscope lamp port is high off the bench so that the High voltage power supply is not lying flat on the ground you should support the High Voltage power supply with an appropriate stand as shown below Note It is not possible to extend the length of the conduit
4. Pulse300 Xenon Flashlamp System designed for use with the Imagex Time Gated Camera The Pulse300 Flashlamp System Introduction The Pulse300 flashlamp is designed to provide microsecond pulses of light across the spectrum from ultraviolet to infrared It is very simple to operate requiring only a 24 volt DC supply The unit dissipates approximately 30 Watts The flashlamp is operated by standard TTL trigger pulses furnished to the BNC input socket The maximum pulse rate is 300 Hz determined by the storage capacitor in the high voltage power supply The system is designed for time gated detection of long lived probes such as lanthanide complexes e g Europium Terbium The light pulses are about a microsecond long with a tail where the light is decaying away of up to 10 microseconds The length of this tail is wavelength dependent and gets longer with increasing wavelength The Pulse300 is specifically designed for use with the Imagex Time Gated camera system and can be driven with a standard TTL pulse The pulse is triggered by a rising edge TTL Low OVolts to TTL High 5 Volts transition and this is supplied by the Lamp ports on the Imagex controller unit Please read the Safety section at the end of this manual as it is very important to observe the correct operational procedures when using the lamp Pulse300 Xenon Flashlamp System Operation Manual Page 1 Description of Components The Pulse300 system is powered
5. This takes mains AC electricity and produces a suitable DC voltage to run the lamp power supply Do not use an alternative power supply without consulting Photonic Research Systems Pulse300 Xenon Flashlamp System Operation Manual Page 3 Attaching the Pulse300 to a microscope The Pulse300 is supplied with a mounting plate and extension tube to allow mounting of the system onto a fluorescence microscope The mounting plate is specific for a particular microscope so it is important to ensure that this plate matches the specification of your microscope lamp mount If you are having difficulty mounting your lamp please contact Photonic Research Systems Before connecting the Pulse300 system to the microscope please ensure that all power supplies are switched OFF lt is a good idea to have the correct tools You may need the following Screwdrivers Pliers A stand with adjustable height An adjustable spanner A set of Allan keys hexagonal screwdrivers Ree ae Please read the Safety section at the end of this manual as it is very important to observe the correct operational procedures when using the lamp You should wear safety glasses during the mounting process and avoid touching the window of the flashlamp 1 Attach the microscope mounting plate A Once you have all the necessary tools you need to locate the microscope mounting plate shown earlier in the Description of Components section This plate provides the int
6. allic gripping tools to remove the bulb from the housing Pulse300 Xenon Flashlamp System Operation Manual Page 10 Safety It is important that you follow the correct safety procedures when operating the Pulse300 lamp Do not attempt to trigger the lamp at gt 300Hz as this may damage the lamp In use an optical filter will normally be needed to select the wavelength range of interest Do not allow the filter to come into contact with the window of the flashlamp or lamp failure is likely The Xenon lamp like all arc sources should be handled with care as it is under pressure Never attempt to remove a lamp until it has cooled to room temperature Never touch the front face of the lamp Fingerprints may cause deterioration of the optical window If the window of the lamp has been inadvertently touched wipe it clean by carefully drawing a lens tissue moistened with ethanol across the face and allow to dry in air before use If the lamp must be removed wear protective gloves and eye protection Do not subject the lamp to lateral forces or torque as this might break the ceramic seal The lamp operates at high voltage but the power supply and input BNC are electrically isolated Ensure that the outer casing of the lamp housing is properly earthed at all times as this improves electrical shielding of emissions and is a further safety precaution An earth point connected to the housing is provided on the power supply box in the form
7. erface between the microscope and the flashlamp head The position of the holes in the mounting plate depend on the microscope that the plate is designed for but the holes to use are usually located around the circular hole in the middle of the plate Use the correct screws to attach the plate to the microscope Attaching the mounting plate onto the microscope lamp port Pulse300 Xenon Flashlamp System Operation Manual Page 4 rOscope mounting plate tached to microscope xtension tube screws e already been inserted Depending upon the geometry of the microscope it may also be necessary to insert M3 screws into the four corner holes as shown before mounting the plate onto the microscope This is only required where the corner holes are obscured after attaching the mounting plate to the microscope See the figure above 2 Attach the extension tube B If you have not yet done so you need to put M3 screws through the corner holes of the mounting plate so that the thread is facing you Pulse300 Xenon Flashlamp System Operation Manual Page 5 Attaching the extension tube to the mounting plate 1st step You may now place the extension tube plate against the mounting plate so that the screws pass through both plates Once you have done this you may now put M3 nuts onto each of the corner screws to bolt the two plates together Attaching the extension tube to the mounting plate Use nuts to bolt the two components
8. ng the holding screws around the circumference of the extension tube and sliding the flashlamp head in or out Retighten the screws once the correct position is obtained The up down left and right positioning is achieved by loosening the four M3 corner nuts that connect the extension tube to the microscope mounting plate Note do not remove the nuts Pulse300 Xenon Flashlamp System Operation Manual Page 9 just loosen them to allow the plates to move You can then slide the two plates across each other until the correct position is obtained Retighten the nuts lock the plates together again Care of your System Once fitted your lamp should not require any significant maintenance If the lamp fails after its normal operating lifetime you may replace it with an identical flashlamp bulb Perkin Elmer FX1164 Please follow the safety instructions on the final page whenever you handle the flashlamp bulb In particular Always wear eye and hand protection The flashlamp bulb is pressurized Ensure that the high voltage power supply is earthed Remove the frequency source before removing installing lamps Never handle or touch the flashlamp until it is cooled to room temperature Ensure that the power supply is switched OFF before removing installing lamps Do not touch the window of the old or new bulb Grease on the window can cause it to fail under pressure Insert the new flashlamp bulb in the same position as the old one Do not use met
9. of a yellow connector Take care not to subject the armoured connection to the lamp to undue strain The short cable length is optimised to give fast optical pulses Do not attempt to install a longer connecting lead to the lamp The power supply unit for the lamp contains components operating at high voltage which could be dangerous Do not open the unit even when the power supply is switched off as the high voltage storage capacitor might still be energised in some circumstances Do not attempt to service this unit In the event of a malfunction return the unit to PRS for repair The light from the flashlamp is rich in short wavelength ultraviolet light which is harmful to skin and eyes Avoid exposure to emission from the unit and use eye protection when operating the lamp Ensure that the lamp and power supply each has adequate ventilation at all times Pulse300 Xenon Flashlamp System Operation Manual Page 11
Download Pdf Manuals
Related Search