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1. Elution 1 Wash 2 Wash 1 Binding reaction Elution 2 Elution 1 Wash 2 Wash 1 Binding reaction Buffer C N OUO A AOD AN O www diagenode com diagendue PAGE 18 DIAGENODE AUTO IPURE KIT V2 USER MANUAL 5 Press the following icon Select the protocol of interest Press start Easy Protocol Start Screen wa Please make sure your selection diagend te Pure 16 samples McIP HLD Pure 16 samples HLD iPure 8 samples McIP HLD lPure 8 samples HLD Pure bisulfite Irina HLD IPure RNA Organic solvent HLD IPure RNA Standard HLD Pure cultured cell HLD IPure Whole blood HLD IPure_PCR amplicon HLD MagPurification DNA from Bisulfite soution3 HLD 6 Before starting the protocol a start confirmation window will appear Press OK and the protocol will run Schedule Manager Ver1 0 ae ii Protacol C Documents and Settings Mazon D esktop iweichenhan protocoles M ew software A Hin Sleep Time 0 Total Sleep Time 0 Max Acton Time 0 Total Action Tine 0 Tithe rio title Active Blocks 1 Tips Reagents Total Process time zer Complete Time 12 53 Prologue bat 0 E file C Documents and Settings M azoni Desktopiweichenhan protocols New software proto gaiii time 0 wl 7 The program will run through the following steps magnetic bead washes IP and IP washes During protocol the next window will be display2. Wash buffer 1 Wash buffer 2 Wash buffer 1w o isopropanol 8 ml Wash buffer 1w o isopropanol 8 ml lsopropanol 100 8 ml lsopropanol 100 8 ml Total volume 16 ml Total volume 16 ml 8 Dispense Wash Buffers 1 amp 2 with Isopropanol in the appropriate container in the IP Star Reagent information B Wash Buffer 1 with Iso propanol ul C Wash Buffer 2 with Iso propanol ul D Buffer C ul 9 Dispense Buffer C in the appropriate container in the IP Star 10 Press Run to start 11 At the end of the run recover your samples on the left block at 4 C End e Open the door e Recover the samples and remove the consumables Left block 200000000 700000000 6 5 4 300000000 200000000 100000000 diageno e 12 Press OK remove the consumables and switch off the IP Star Reagent information A lsopropanol ul B Wash Buffer 1 with Iso propanol ul C Wash Buffer 2 with Iso propanol ul 13 Place the DNA on ice and proceed to any desired downstream applications or store it at 20 C or 80 C until further USG www diagenode com diagendtie How to perform Automated Pure on the IP Star IP STAR PAGE 15 How to perform Automated IPure on the IP Star Compact Auto Pure is done in 96 well plates placed in the room temperature modules of the IP Star Compact Each 96 well plate will have capacity to run 8 or16 Pur
3. PN Pra Je y 4 OLATA iN h a a OO Sample prep hMeDIP Re ChIP MagBisulfite RNA IP r d MethylCap rA Library prep Om IPure diagenc e User interface Intuitive touch screen panel PC Software User friendly Software training not required Software training before use Dispensing Automated dispension of assay reagents Manual dispension of assay reagents Antibody coating temperature time mixin Protocol y g P J ero speed optimization ee Antibody coating temperature time Immunoprecipitation temperature time pd tae flexible Immunoprecipitation temperature time mixing speed parameters New protocol development Washes temperature time mixing speed Achievable by Diagenode product specialist Achievable by customer after training Characteristics 750W x 740 D x 610 H 100 kg 8 Nozzles X Y Z axis 4 95 C 1070W x 650 D x 780 H 130 kg 8 Nozzles X Y Z axs 4 95 C Innovating Epigenetic Solutions PAGE 7 Improved reproducibility Our IP Star will increase the immunoprecipitation reproducibility between IPs performed by the same as well as by different operators see figure 1 and 2 below Reagents Antibodies buffers and sheared chromatin were identical for ManChIP and AutoChIP The IP Star Automated system removes variation that can be created by manual handling and allows you to optimize a
4. rxns Auto MeDIP kit x16 C02010011 16 rxns Auto MeDIP kit x100 C02010012 100 rxns www diagenode com diagendtie How to perform Automated Pure on the IP Star Compact IP STAR COMPACT PAGE 11 How to perform Automated IPure on the IP Star Compact Auto Pure is done in 96 well plates placed in the room temperature modules of the IP Star Compact Each 96 well plate will have capacity to run 8 16 or 24 IPure samples ChIP and MeDIP Elution buffer To perform DNA purification with Auto IPure elution step after Auto Histone ChIP seg Auto Plant ChIP seg and Auto MeDIP kits must be done using the Buffer A and B provided in the Auto Pure kit v2 or Elution Buffer 1 and 2 provided In the ChIP or MeDIP kits Elution buffer iexns Buffer A 115 4 ul Buffer B 4 6 ul Total volume 120 ul volume is calculating with 20 of excess 1 Select Protocols icon and then IPure category Protocols Pure_08 IPure_16 IPure_24 Pure seq_08 Pure seq_16 Pure seq_24 NOTE If you plan to run between 1 and 8 samples choose IPure_08 or IPure seq_08 If you plan to run between 9 and 16 samples choose IPure_16 or IPure seq_16 If you plan to run between 17 and 24 samples choose IPure_24 or IPure seq_24 www diagenode com diagendtie PAGE 12 DIAGENODE AUTO IPURE KIT V2 USER MANUAL 3 Setup the exact number of samples for your ex
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6. E ate eta oe eee ee ence a ceases aaa ere tie ese beara 21 TS CMAIG AU ASSIS CAN CC aona AEE tue tie dacs used AN EA 21 www diagenode com diagendtie PAGE 4 DIAGENODE AUTO IPURE KIT V2 USER MANUAL Introduction The Diagenode IP Star Automated System automates immunoprecipitation and increases reproducibility Diagenode the leading provider of complete solutions for epigenetics research offers a variety of end to end systems to streamline DNA methylation and chromatin immunoprecipitation workflows Central to this full offering is Diagenode s Automated Systems simple yet robust automated bench top instruments that standardize different epigenetic applications i e ChIP MeDIP or MethylCap Diagenode designed these automation systems to make ChIP and DNA methylation studies accessible and reproducible and ensure consistent data in every experiment Diagenode Automated Systems will produce consistent results from any operator regardless of the day the experimental run or the lab Robust and reproducible results is a major goal of today s high resolution epigenomic studies Diagenode Automated Platforms replace the numerous manual error prone steps of complex epigenetic applications with a reliable highly consistent and automated process that requires minimal operator intervention We empower researchers to simplify the tedious protocols and the complexity of many epigenetic protocols In addition Diagenode Automated Systems minimiz
7. NODE AUTO IPURE KIT V2 USER MANUAL Kit method overview Pure after ChIP STEP 1 Chromatin reverse cross linking and elution Chromatin is decrosslinked and eluted from magnetic beads ri s magnetic or agarose which are So d F a discarded O ka Magnetic beads for purification are added STEP 2 DNA binding Magnetic beads acquire positive charge Q to bind negatively charged phosphate R gt backbone of DNA te DNA bead complex is separated using a 7 magnet STEP 3 Washes A Proteins and antibody debris are sA washed away p STEP 4 DNA Elution DNA is eluted from magnetic beads which are discarded o gt Purified DNA is ready for any downs tream application qPCR next generation sequencing amplification microarray Pure after MeDIP STEP 1 DNA Elution DNA is eluted from beads magnetic or agarose Magnetic beads for purification are added STEP 2 DNA binding Magnetic Beads acquire positive charge to bind negatively charged phosphate backbone of DNA DNA bead complex is separated by using a magnet STEP 3 Washes Proteins and antibody debris are washed away STEP 4 DNA Elution DNA is eluted from magnetic beads which are discarded Purified DNA is ready for any downs tream application qPCR next generation sequencing amplification microarray Innovating Epigenetic Solutions Kit materials Kit content The kit content is sufficient to
8. cription Factors x24 C01010058 24 rxns Auto Plant ChIP seq kit x24 C01010151 24 rxns Auto MeDIP kit x16 C02010011 AF Auto01 0016 16 rxns Auto MeDIP kit x100 C02010012 AF Auto01 0100 100 rxns iDeal Library Preparation Kit x24 incl Index Primer Set 1 C05010020 24 rxns Auto hMeDIP kit x16 C02010033 AF Auto02 0016 16 rxns Auto MethylCap x48 C02020011 AF Auto01 0048 48 rxns Auto IPure kit v2 x100 C03010010 AL Auto01 0100 100 rxns Visit us at one of Diagenode s demo sites or discover our Automated Systems by performing some assays with the help of our R amp D and Technical Department www diagenode com DIAGENODE S A DIAGENODE INC FOR A COMPLETE LISTING OF BELGIUM EUROPE LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Belgium Tel 32 4 364 20 50 Fax 32 4 364 20 51 orders diagenode com info diagenode com USA NORTH AMERICA 400 Morris Avenue Suite 101 Denville NJ 07834 Tel 1 862 209 4680 Fax 1 862 209 4681 orders na diagenode com info na diagenode com DIAGENODE S INTERNATIONAL DISTRIBUTORS VISIT http www diagenode com company distributors php For rest of the world please contact Diagenode sa www diagenode com DIAGENODE HEADQUARTERS 2015 Diagenode Inc All rights reserved The content of this document cannot be reproduced without prior permission of the authors Bioruptor amp IP Star are registered trademarks of Diagenode MA_Auto iDealChIPseqIF V2_0
9. diageng e Innovating Epigenetic Solutions Auto IPure kit V2 Magnetic DNA Purification kit for epigenetic applications Cat No C03010010 100 rxns Version 2 03 15 Contacts DIAGENODE HEADQUARTERS Diagenode s a BELGIUM EUROPE LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Belgium Tel 32 4 364 20 50 Fax 32 4 364 20 51 orders diagenode com info diagenode com Diagenode Inc USA NORTH AMERICA 400 Morris Avenue Suite 101 Denville NJ 07834 Tel 1 862 209 4680 Fax 1 862 209 4681 orders na diagenode com info na diagenode com For a complete listing of Diagenode s international distributors visit http www diagenode com company distributors php For rest of the world please contact Diagenode sa Diagenode website www diagenode com PAGE 3 Content WE OUI C TOs agace cece A EA E A oie A E E E E pone acecaseesamearcaniiciasag E RS 4 IP Star and IP Star Compact Systems for automation of epigenetic applications 5 Kit method VT VU aeaieie nace sans gt vn sehen te nn ores SA deen onan E EO hates teen tenants 8 FUE MVE TANG PEAS EE AIEA AEAEE TTEA au uted yyoau uns deese sens iad aseseantepas OEE OE TETS 9 How to perform automated IPure on the IP Star Compact a 10 How to perform automated IPure onthe IP Stare occ cece vce ecb cee rete vet eteretreteverreee 14 FSS pac pce ee em we essere se ss es eee epee cet aes ee pe ne a eee ents ena 20 TM URED SUV OUI NG guide ct pa
10. e sample carryover data variability and costly errors The platforms offer full workflow support for epigenetics research utilizing our complete kits and laboratory validated protocols to rapidly deliver high quality and consistent data Auto IPure kit V2 Diagenode s Auto Pure kit v2 is the only DNA purification kit using magnetic beads that is specifically optimized for extracting DNA from ChIP and MeDIP Chromatin IP and Methylated DNA IP experiments Its a simple and straightforward protocol that delivers pure DNA ready for any downstream application e g next generation sequencing This approach guarantees a minimal loss of DNA and reaches significantly higher yields than a column purification see results page Comparing to phenol chloroform extraction the Pure technology has the advantage of being nontoxic and much easier to be carried out on multiple samples The use of the magnetic beads allows for a clear separation of DNA and increases therefore the reproducibility of your DNA purification Diagenode s IP Star system uses the principle of bead based magnetic separation Magnetic beads bound with chromatin or DNA are brought T to the inner wall of the tip when a strong magnetic force is applied This differs from other systems that collect the bound DNA on the bottom of a reaction well resulting in cleaner assays and less carryover Innovating Epigenetic Solutions PAGE 5 IP Star and IP Star Compact Systems for au
11. e samples A ChIP and MeDIP Elution buffer To perform DNA purification with Auto IPure elution steps after Auto ChIP and Auto MeDIP must be done using the Elution Buffer provided in the Auto Pure kit v2 Elution buffer Pens Buffer A 115 4 ul Buffer B 4 6 ul Total volume 120 ul volume is calculating with 20 of excess 100 ul Elution Buffer per sample are needed per IP or input sample to perform the elution ChIP MeDIP Elution Buffer provided in the Pure kit will be used as indicated in the Auto ChIP and Auto MeDIP user manuals B Prepare Auto Pure kit buffers Add as indicated below the suggested isopropanol volumes to the corresponding Auto IPure kit v2 buffers Wash Buffer 1 Wash buffer 1 100 rxns Wash buffer 1 w o iso propanol 8 ml Iso propanol 8 ml Total volume 16 ml Wash Buffer 2 Wash buffer 2 100 rxns Wash buffer 2 w o iso propanol 8 ml Iso propanol 8 ml Total volume 16 ml www diagenode com diagendtie PAGE 16 DIAGENODE AUTO IPURE KIT V2 USER MANUAL C Dispense prepared reagents in 96 well plate 12 Elution 2 11 Elution 1 10 f Wash 2 9 Wash 1 8 Binding reaction 7 6 Elution 2 to Sampieg 5 Elution 1 4 OOOO vs 0000000 v ia 2 00000 _ Binding reaction 1 C OOOOCE icc Se Co wtiet te a_i Well Description Volumes 12 Buffer C to be dispensed by the IP Star LIN 11 Buff
12. ed indicating the current protocol step Innovating Epigenetic Solutions PAGE 19 Running Protocol The current date and time gt Tue May 25 12 56 21 2010 pote a Sara 1 1 1 0 1 1 2 Well information 0 1 1 3 0 s Sa 1 2 1 New Tip Collect 0 1 1 3 5 Binding reaction 0 1 3 1 vVell 1 Start Watch 1 3 2 Bottom Sense 0 Stack PUSHI1 40 Mix volume ul 1 3 3 Pre asp Repeat 1 3 4 Beads resuspend t Vwait_msec 1000fwaittime msec 1 3 5 Binding reaction 0 Stack DUP 1 3 6 Air Dsp Liq_in POP P_SPEED_H Asp Speed 1 3 7 Interium Height Z Move Wait_msec 200 waittime msec 1 3 8 Tip Discard Stack DUP 1 4 1 New Tip Collect Liq_out POP P_SPEED_H Disp Speed 1 5 1 Assay PlatetvYell 0 Pass_time 1 5 2 Z position IF_Goto LE 1800 Mixing Time Sec Repeat 1 5 3 Asp 30ul Stack Drop 1 5 4 Z up 1 5 5 Interium Height Z Move 1 6 1 Assay plate vVell4 1 6 2 Bottom Sense 0 1 6 3 Disp BufferA 1 6 4 Disp air 1 6 5 Interium Height Z Move 1 7 1 Assay PlatetvYell 0 1 7 2 Z position 1 7 3 Asp 30ul 1 7 4 Z up 1 7 5 Interium Height Z Move 1 8 1 Assay plate vVell5 1 8 2 Bottom Sense 0 1 8 3 Disp BufferA 1 8 4 Disp air 1 8 5 Interium Height Z Move 1 1 8 6 Tip Discard 5l _ amity ene 9 9 The IP Star software indicates the end of the pro
13. er C to be dispensed by the IP Star Zo ul 10 Wash Buffer 2 100 ul 7 Wash Buffer 1 100 ul 8 Isopropanol beads carrier 100 ul 10 ul 2 ul 100 ul vi empty 6 Buffer C to be dispensed by the IP Star 20 ul J Buffer C to be dispensed by the IP Star 20 ul 4 Wash Buffer 2 100 ul 3 Wash Buffer 1 100 ul 2 Isopropanol beads carrier sample 100 ul 10 ul 2 ul 100 ul 1 Buffer C 150 ul Alternatively elution can be done in 150 ul followed by ethanol precipitation if final volume must be reduced Sample can be IP sample or input Innovating Epigenetic Solutions PAGE 17 D Loading and running protocol Be sure that the computer connected to the robot never switches to the standby modus standby modus has to be inactivated Standby of the computer will lead to the abort of the protocol Protocol Name Auto Pure Reagent Preparation 15 min Binding reaction 30 min Washes 20 min Elution 30 min Total Time 1 30 min per 8 samples 1 Switch on the SX 8G IP Star The power switch is on the right side of the instrument 2 Switch on the computer 3 Start SX 8G V52 software through SX 8G V32 the following icon 4 Place the prepared 96 well plate in the indicated room temperature module in the workstation eee a eee Sey Poem gee atel stat akay tt Pho Ro Re Rebeteto tal faled eneanneeesea seascaesese Bi pp pp p serere LELLE T EF i he ttt ttt 12 11 10 Elution 2
14. nd standardize your assay within a lab The IP Star is designed to improve the accuracy and the reproducibility of any immunoprecipitiation experiment SD IgG 0 69 Figure 1 Manual ChIP Man ChIP SD H3K9me3 23 84 j Four different operators have each performed two ChIP experiments using H3K9me3 antibody on the SD IgG 1 4 ee SDIH3K9me3 2 38 genomic region SAT2 positive locus 10 000 Hela C 4 sptigele0 94 SD IgGI 0 17 a E cells have been used per IP Reagents and sheared re sie 11 36 E 1 12 cae e A a 0 45 chromatin were identical per assay The standard sige deviations between the ChIPs performed by the ChIP 1 ChIP 2 ChIP 1 ChIP 2 ChIP 1 ChIP 2 dae _ 5783 56 25 same operator and between the four different i 43 83 44 75 i Say i operators are displayed 20 0 7 hae 1o ai ka Auto ChIP SD IgG 0 28 Figure 2 Automated ChIP SD H3K9me3 1 6 l l Four ChIP experiments using H3K9me3 antibody on 100 0 the genomic region SAT2 positive locus have been 90 0 _ aaa performed by the P Star 10 000 Hela cells have g 7004 ChIP 1 ChIP 2 ChIP 3 ChIP 4 been used per IP Reagents and sheared chromatin E 60 0 a uae sis were identical per assay The standard deviations S 50 0 4 ne between the four ChIPs performed by the P Star 30 0 are displayed 20 0 10 0 _ 1 26 1 00 1 45 0 81 IgG H3K9me3 IgG H3K9me3 IgG H3K9me3 IgG H3K9me3 www diagenode com diagendtie PAGE 8 DIAGE
15. nts were run in the IP Star following Diagenode s protocols The IP was performed by including the kit internal controls together with the human DNA sample The internal positive and negative DNA controls included in the IP assay are methylated DNA meDNA and unmethylated DNA unDNAJ As positive and negative control regions a non methylated region in the GAPDH promoter and the methylated region of TSH2B were tested Results showed 4 different AutoMeDIP Auto IPure experiments run in the IP Star automated system Innovating Epigenetic Solutions PAGE 21 Troubleshooting guide Error Cause Remedy SX 8G P Star is not receiving power Check that the power cord is connected to the SE Nee Oe Neen workstation and to the wall power outlet Computer is not receiving power Check that the power cord is connected to the e CANOE E O computer and to the wall power outlet SX 8G IP Star shows no movement when a protocol is Sterten SX 8G IP Star is not switched on Check that the SX 8G IP Star is switched on The pipettor head may have lost its home position In the Software select Manual Operation Home After confirming that the pipettor head moves to the home position run the protocol again SX 8G IP Star shows abnormal movement when a protocol is started Dripping is acceptable when ethanol is being handled For other liquids air is leaking Aspirated liquid drips from the disposable tips from the syringe pumps G
16. ore Inc Illumina is a registered trademark of Illumina Inc lon Torrent and Personal Genome Machine are trademarks of Life Technologies Corporation Qubit is a registered trademark of Life Technologies Corporation Innovating Epigenetic Solutions Ordering information diageng e Innovating Epigenetic Solutions Products Cat No new Cat No old Format IP Star Compact B03000002 UH 002 0001 1 unit Auto True MicroChIP kit x16 C01010140 16 rxns Auto True MicroChIP amp MicroPlex Library Preparation Package C01010141 16 ChIP rxns amp 12 library prep rxns MicroPlex Library Preparation kit x12 C05010010 AB 004 0012 12 rxns MicroPlex Library Preparation kit x48 C05010011 48 rxns Auto Histone ChIP seq kit protein A x16 C01010020 AB Auto02 A016 16 rxns Auto Histone ChIP seq kit protein A x100 C01010022 AB Auto02 A100 100 rxns Auto Histone ChIP seqg kit protein G x16 C01010021 AB Auto02 G016 16 rxns Auto Histone ChIP seq kit protein G x100 C01010023 AB Auto02 G100 100 rxns Auto iDeal ChIP seqg kit for Histones x 24 C01010057 24 rxns Auto Transcription ChIP kit protein A x16 C01010030 AB Auto03 A016 16 rxns Auto Transcription ChIP kit protein A x100 C01010032 AB Auto03 A100 100 rxns Auto Transcription ChIP kit protein G x16 C01010031 AB Auto03 G016 16 rxns Auto Transcription ChIP kit protein G x100 C01010033 AB Auto03 G100 100 rxns Auto iDeal ChIP seqg kit for Trans
17. perform 100 reactions PAGE 9 Pure kit 100 reactions Description Format Storage 96 well microplates 10 pe Room temperature Buffer A 15 ml 4 C Buffer B 600 ul eae Wash buffer 1 w o iso propanol 8 ml 4 C Wash buffer 2 w o iso propanol 8 ml aC Buffer C 8 ml 4 C Magnetic beads 1 1 ml 4 C Carrier 300 ul 20 C This product is shipped at 4 C Store it at 20 C upon arrival Plastics and consumables available separately Description Cat No Format 200 ul tube strips 12 tubes strip cap strips C30020001 80 aed strips 8 tubes strip cap strips for IP Star 630020002 120 96 well microplates for IP Star C30080030 10 Tips box C30040021 960 Tips bulk C30040020 1000 2 ml microtube for IP Star Compact C30010014 100 Large reagent container for IP Star Compact C30020004 20 Medium reagent container for IP Star Compact C30020003 10 Kits and Modules available separately Description Reference Quantity Chromatin shearing optimization kit Low SDS C01020010 1 kit Chromatin shearing optimization kit Medium SDS C01020011 1 kit Chromatin shearing optimization kit High SDS C01020012 1 kit Auto Histone ChIP seq kit protein A x16 C01010020 16 rxns Auto Histone ChIP seq kit protein A x100 C01010022 100 rxns Auto Histone ChIP seq kit protein G x16 C01010021 16 rxns Auto Histone ChIP seq kit protein G x100 C01010023 100
18. periment Each IP and input has to be counted as a sample NOTE The Peltier Block is now cooling down to 4 C to keep your samples cold 4 Setup all the plastics on the platform according to the screen layout o eoeeeoee eeeeeeed 2e eeeeeee0 eeeeoeoeed e eeeeeeee eeoeoeeoee eeeoeeoeee eeeeeeee eeoeoeeeeo eeeseee eeeeeoee 100000000 UE AE AA 4d Reagent sagen soeeocece Rack 1 soeseecee 2000000080 Sei ei elite 96 plate 1 196 plate 2 06090000A 80002900 80 YYYY 00000080 ALLIK 0000008 0002020028 0009090990 Eam PCR tube Empty for products 17 24 Empty for products 9 16 Empty for products 1 8 Samples 17 24 100ul carrier 2yl Samples 9 16 100 carrier 2ul Samples 1 8 100pl carrier 2ul 6 Resuspend and dispense 10 ul of magnetic beads IPure for each sample on the 96 well plate 96 plate 198A OOO round bottom OAOAOAeneene om AAO 444 Sample number 0 amp ARAADHA E yy vy YY oh AAA AA A Aa 9 OOA0OOOO Fy yy Fy Fy a WV WW WW VW C 68 Oa Wi oO NOTE Keep the magnetic beads in liquid suspension during storage at 4 C and at all handling steps as drying will result in reduced performance Make sure the beads are homogeneously in suspension at all the time during pipetting steps because the beads are precipitating rapidly Innovating Epigenetic Solutions 7 Dilute Wash Buffers 1 1 with isopropanol PAGE 13
19. r any animal or human therapeutic or diagnostic use 2015 Diagenode SA All rights reserved No part of this publication may be reproduced transmitted transcribed stored in retrieval systems or translated into any language or computer language In any form or by any means elec tronic mechanical magnetic optical chemical manual or otherwise without prior written permission from Diagenode SA hereinafter Diagenode The information in this guide is subject to change without notice Diagenode and or its affiliates reserve the right to change products and services at any time to Incorporate the latest technological develop ments Although this guide has been prepared with every precaution to ensure accuracy Diagenode and or its affiliates assume no Liability for any errors or omissions nor for any damages resulting from the application or use of this informa tion Diagenode welcomes customer Input on corrections and suggestions for improvement NOTICE TO PURCHASER LIMITED LICENSE The information provided herein is owned by Diagenode and or its affiliates Subject to the terms and conditions that govern your use of such products and information Diagenode and or its affiliates grant you a nonexclusive nontransfer able non sublicensable license to use such products and information only in accordance with the manuals and written instructions provided by Diagenode and or its affiliates You understand and agree that except as expressl
20. rease or replace the O rings If the problem persists contact DIAGENODE Technical Services Technical assistance At DIAGENODE we pride ourselves on the quality and availability of our technical support Our Technical Services Departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of DIAGENODE products If you have any questions or experience any difficulties regarding the SX 8G IP Star or DIAGENODE products in general do not hesitate to contact us DIAGENODE customers are a major source of information regarding advanced or specialized uses of our products This information is helpful to other scientists as well as to the researchers at DIAGENODE We therefore encourage you to contact us If you have any suggestions about product performance or new applications and techniques For technical assistance and more information call the DIAGENODE Technical Service Department or contact your local distributor Please do not hesitate to contact our customer support team if you have any questions about the design of your ChIP seq experiment or the bioinformatics data analysis Contact for Europe Asia Oceania and Africa al custsupport diagenode com Contact for North and South America ASK THE custsupport na diagenode com EXPERTS www diagenode com diagendtie PAGE 22 DIAGENODE AUTO IPURE KIT V2 USER MANUAL FOR RESEARCH USE ONLY Not intended fo
21. tocol Press the close buttom to finish the protocol run IPure 16 samples McIP HLD IPure 16 samples HLD Pure 8 samples McIP HLD sHLD Pure bisulfite Irina HLD IPure RNA Organic solvent HLD IPure RNA Standard HLD Pure cultured cell HLD IPure Whole blood HLD IPure_PCR amplicon HLD MagPurification DNA from Bisulfite soution3 HLO 10 Collect your purified DNA This is your DNA ready for gPCR Shutting down the IP Star 1 Click on File and press End to close the software correctly 2 Switch off the computer and its monitor 3 Switch off the IP Star Robot power switch on the right side Note Ensure that the door is closed www diagenode com diagenotie PAGE 20 DIAGENODE AUTO IPURE KIT V2 USER MANUAL Results Comparison of DNA recovery after purification with Pure technology and competitor kits Purification efficiency 100 Auto IPure v2 Competitor Z Competitor Q DNA recovery after purification of MeDIP samples using IPure technology a MeDIP Auto Pure E meDNA m unDNA m TSH2B m GAPDH 100 90 80 70 60 50 40 30 of input 20 10 0 MeDIP 1 MeDIP 2 MeDIP 3 MeDIP 4 Xe By Methyl DNA IP results obtained with our Auto MeDIP Kit and after DNA purification using the Auto Pure kit v2 Methyl DNA IP assays were performed using DNA from U20S cells and the Auto MeDIP kit Diagenode After MeDIP the DNA was purified using the Auto IPure kit v2 Experime
22. tomation of epigenetic applications Diagenode has developed two automated platforms IP Star and IP Star Compact designed to increase your lab s productivity efficiency and experimental reproducibility The two automated platforms are capable of processing up to 16 samples per cycle The automated systems processes sheared chromatin or DNA to deliver purified DNA ready for qPCR amplification microarray and sequencing analysis Both the IP Star and IP star Compact have an easy to use open software that provides you with flexibility This allows you to create your personal protocol according to your specific needs Major benefits of Diagenode Automated Platforms IP Star Compact P Star pi High resolution ChIP seq and MeDIP seq profiles Automated library preparation for Next Generation sequencing Reduces hands on time to just 30 minutes Reduces variability between operators and labs Ideal for low sample starting amounts Compatible with Diagenode Kits VY VUV VY Vv Vv y4 Reduces cross contamination www diagenode com diagendtie DIAGENODE AUTO IPURE KIT V2 USER MANUAL IP Star Compact IP Star Applications ChIP seg MeDIP seg MethylCap seg hMeDIP IPure Sample preparation Re ChIP MagBisulfite RNA IP Library preparation for NGS platforms ChIP seg MeDIP seg MethylCap seq hMeDIP IPure Sample preparation Re ChIP MagBisulfite RNA IP Software Protocols z gt
23. y set forth in the terms and conditions governing your use of such products that no right or license to any patent or other intellectual property owned or licensable by Diagenode and or its affiliates is conveyed or implied by providing these products In particular no right or license Is conveyed or implied to use these products in combination with any product not provided or licensed to you by Diagenode and or its affiliates for such use Limited Use Label License Research Use Only The purchase of this product conveys to the purchaser the limited non transferable right to use the product only to perform internal research for the sole benefit of the purchaser No right to resell this product or any of its components is conveyed expressly by implication or by estoppel This product is for internal research purposes only and is not for use In commercial applications of any kind including without limitation quality control and commercial services such as reporting the results of purchaser s activities for a fee or other form of consideration For information on obtaining additional rights please contact infolddiagenode com TRADEMARKS The trademarks mentioned herein are the property of Diagenode or their respective owners Bioruptor is a registered trademark of Diagenode SA Bioanalyzer is a trademark of Agilent Technologies Inc Agencourt and AMPure are registered trademarks of Beckman Coulter Inc Microcon is a registered trademark of Millip
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