1 148 Series User`s Manual
Contents
1. User s Manual Table of Contents A E AA P 171077101 o MNT RI HQ e 3 Safety PF CRIDIONS a stes tena anaa E a aeiae 4 AUS A A non AA 5 AD E AA 5 Definition of Parts A A eiiieaen 6 Cae En A 7 A Cu HR 7 OperatjOomnf J aca T COLOR BOE D D Dm 8 Selling Om E E B HU Cee Ae ee EL 8 F bubigo P eue AF IA eut Peut O ee A ee AA 8 Adjusting Theb TUE m tL eere 8 Attaching a Camera Chad v UBE VEDUIEUEBBN aeneo peer raten etse nies 8 Rayo Uo an a RM 9 Pe es D EFT uM Tension BRI 9 Mamtenanc Precautions NENNEN 10 Pe EOS O NU AEE E ed 11 NAS Series PEC 11 Optional ACES is 12 Y CS i3 122 71 A 13 Tecanical PALACE ES ein isis 14 Electrical SYSTEM ME P e 14 Technical Terms amp Eg T E eii 15 COMMON ISSUES T 16 Common Issues Continued rr 17 Before Use Introduction Congratulations on the purchase of your new AmScope microscope This manual is designed for the M148 series microscopes Please be sure to take a few minutes to familiarize yourself with the features and functions of your new AmScope microscope If youd like more information on microscopes parts or accessories please visit our website at www iScopeCorp com We highly recommend you study this manual thoroughly befor2. is calculated by the magnification of the objective multiplied by the magnification of the eyepieces Ex 10x Eyepieces x 4x Objective 40x Total Magnification Field of View Linear field of view of the eyepiece divided by the magnification of the objective Numerical Aperture N A Calculated by n Sin a max the Numerical Aperture N A is an important parameter that marks the fe 19902 taures of the objective and condenser s image quality and resolution The n is a refractive index of the medium air or immersion cedar oil between the omjective lens and the specimen The a is 1 2 of the angle between the aperture on the objective and path of light The larger the N A the higher the resolution of the objective and better quality of the image Object to Primary Image Distance The distance between the object plane and the primary image plane The conjugate distance is fixed Mechanical Tube Length The distane between the objective shoulder and the ocular shoulder 15 Troubleshooting Common Issues OPTICAL ISSUES One side of the field The nosepiece if misaligned Turn the nosepiece until it clicks into place of view is darker Stains or dust has accumulated on the objective Clean all lenses with lens cleaner or a lint free non eyepieces or base lens abraisive cloth Obstructions are Stains dust or dirt has accumulated on the speci Clean the slide or use a new specimen if sample is observed in
3. to avoid damaging the slide and objective Nosepiece Houses the objective lenses Rotating Monocular Head Houses the eyepiece and optics of the microscope Getting Started Assembly 1 First take the styrofoam container out of the cardboard carton and lay it on its side paying attention to which side is labeled up Remove the tape and open the container carefully so as to avoid dropping and damaging the optical items Check carefully to ensure that all parts and accessories are intact 2 Check the packing list to ensure that you re received all items One Monocular Microscope Body amp Head Three Objectives 4x 10x 40x 10x Widefield Eyepieces 16x Widefield Eyepieces for A models only 20x Widefield Eyepieces for B models only 25x Widefield Eyepieces for C models only One Dust Cover Note LED models do not have a spare bulb as the LED bulbs do not need replacing 3 Remove the microscope body from the box and remove the plastic protective covering The body of the micro scope is composed of the base the stage the arm and the nosepiece 4 Screw the objectives into the microscope nosepiece from the lowest magnification to the highest again avoid ing touching the lenses 5 Plug in the microscope and turn it on If no light emerges from the light source adjust the dimmer knob on the side of the base Operation Setting Up 1 Place the specimen to be studied on a glass slide or use a prepar
4. cover slip The limit stop is set too high or not engaged Be careful to avoid contact between objective and the slide when the limit stop is not engaged To reengage focus the sample then lock the limit stop into place to set max height at a safe but us able distance Unable to move the slide The slide is not secured correctly Adjust the slide to use the stage clips and secure the sample smoothly The mechanical stage is not properly secured Tighten the mechanical stage screws to better secure the stage Focus knob does not turn The tension knob is too tight Loosen it by adjusting the tension ring inside the coarse focus knob counterclockwise close to the arm of the microscope on the left of the microscope Stage declines by itself The tension knob is too lose Tighten it by adjusting the tension ring inside the coarse focus knob clockwise close to the arm of the microscope on the left of the microscope The focusing knob wont Limit stop is engaged Disengage the limit stop on the rear of the stage of the micro raise the stage scope ELECTRICAL ISSUES The bulb light source The bulb is close to burning out Please contact us in regards to this issue LED lights do not burn flickers out so there may be another electrical issue The microscope does not The microscope is unplugged Insert the plug into the wall socket to achieve electrical illumina light up tion E
5. desired aperture Attaching a Camera Changing Eyepieces 1 To remove the 10x eyepiece included on the microscope you will need to remove the small metal screw on the ocular tube just under where the eyepiece connects to the tube To do so you will need a 1mm flathead precision screwdriver much like what you would use to adjust eye glasses 2 Once unscrewed remove the eyepiece and slide in the camera or alternate eyepieces Operation Setting the Stage s Stop Limit 1 To adjust the stop limit on the stage unlock it by unscrewing the lower nut The stop limit is located at the rear of the stage 2 Once tension is released on the nut by rotating it counter clockwise you may adjust the upper thumb screw to move the limit up or down as desired 3 Lock the limit stop nut back into place after the desired stage height is achieved Operation Maintenance Precautions All glass surfaces must be kept clean Fine dust on the optical surface should be blown off using a can of com pressed air or gently wiped off with a soft lens paper tissue nonabraisive lint free cloth Carefully wipe off oil or fingerprints on the lens surfaces using tissue moistened with a small amount of lens cleaner we recommend Sparkle brand optical cleaner Do not use Sparkle to clean other elements of the microscope Use a neutral detergent on any plastic or painted surfaces Do not assemble or disassemble the microscopes electrical com
6. e operating the microscope and that yo ukeep it on ahnd for future reference If you have additional questions or need assistance please send us an email at info amscope com Before Use Safety Precautions 1 As the microscope is a precision instrument always handle it with care avoiding impact or abrupt movement during transporation Do not shake the package 2 Do not place the microscope in direct sunlight or in high heat Keap it indoors in a dry and clean place with temperateures between 32 100 degrees F 0 40 degrees C and in maximum relative humidity of 85 3 Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from your fingerprints do not obstruct your view 4 Before turning the power on make sure that the power supply voltage is consident with the voltage of your microscope M148 Eyepiece Ocular Tube Rotating Monocular Head Nosepiece Turret Y p Limit Stop d Objectives Stage Clips Stage Disc Diaphragm Disc Iris _ Focusing Knob Illuminator Lens Definition of Parts Base Illuminator Lens Directs the light source towards the slide Focusing Knob Used to bring the slide into the sight and focus Disc Iris Diaphragm Controls the amount of light that hits the slide from the base illuminator Limit Stop Knob Limits the upward movement of the mechanical stage in order
7. ed slide Place it on the stage holding it snugly in place with the metal slide holders clips of the mechanical stage 2 Center the specimen over the stage opening lining it up with the light and the objective lens Focusing 1 Turn the nosepiece to choose an objective It is easiest to use the lowest magnification first 4x objective to lo cate and focus on the specimen As you move up in magnification you may need to refocus the image a little each time 2 Being focusing by first looking with one eye through the eyepiece without the diopter Close your other eye Use the coarse focusing knob to adjust the height of the stage until the sample comes into clear focus Note You may loosen the limit stop knob located on the read of the stage in order to give yourself the full range of motion for fine tuning the focus 3 Once the image is clear in your field of view you will want to use the fine focusing knob to tune it for best results Note Please be careful when moving the mechanical stage if you need to recenter the sample or if moving the stage very close to the objectives The limit stop is designed to prevent impact between objective and slide so when it is off you will be able to damage the microscope Adjusting The Diaphragm 1 By changing the aperture hole size of the iris diaphragm you can adjust the background brightness Adjust the aperture of the iris diaphragm by rotating the disc underneath the stage to the
8. ponents yourself without advisement from one of our technicians Doing so will void your warranty unless by advisement of one of our technicians to do so After use cover the microscope with the provided dust cover Keep your AmScope microscope in a dry clean location in order to prevent rust or other damage To change batteries with this unit use the included allen wrench hexagonal shaped L tool to undo the door hex screw on the base Use 3 AAA batteries with the unit The unit can be used as a recharger for AAA batteries specified as rechargeable only Please do not use standard batteries in the unit with the charger plugged in to avoid damage 10 Specifications 148 Series Specifications M148 MI4SB MI48C M148A WE Eyepiece WF25X18mm Plan Objectives 40X spring Plan Eyepiece 40X spring 0 65 100X spring oil 1 25 DIN Achromatic Objectives 60X spring 0 85 45 Degree Viewing Head Illumination Halogen Light w Dimmer Lamp 6V 20W 6V 30W x x x X X BR x x x m X x x x x x x tal tal ES mn Te gt tal tal 11 Specifications Optional Accessories Eyepiece 5X EP5X18 Obtaining 20x 50x 200x and 500x magnification powers 20x EP20X18 Obtaining 80x 200x 800x and 2000x magnifica tion powers 25x EP25X18 For obtaining 250x and 2500x magnification pow ers Camera CMOS Digital MU035 350k To capture images video or view live display on a MU130 1 3mp compute
9. r PC Mac OS X MU300 3mp MU500 5mp MU800 8mp MU900 9mp MU1000 10mp Calibration Micrometer MR400 To calibrate the camera software for on screen measurements CCD TV Video CCD NP To view live display on a television RCA AC B100 For carrying microscope around safely 12 Specifications Objectives Type Magnification Numerical Aperture Medium Parfocal Distance mm Magnification Marks EE ANE A Color a DIN Achromatic a A A OA a ar e rage 195mm Eyepieces Type Widefield Eyepiece Plan Eyepiece Medium 13 Technical Parameters Electrical System There are two options for eletrical systems for this series of microscope The light source is an LED system 1 220V 240V power supply 220V 240V 10 50Hz This electrical system is CE and GS certified 2 100V 120V power supply 100V 120V 10 60Hz This electrical system is UL certified All units come standard as 110V units unless an upgrade to a 220V system is requested Upgrade fee is dependent on which unit is purchased This unit is also capable of using batteries and charging rechargable batteries It uses 3 AAA batteries When plugged in with batteries in the unit the batteries will be charged Note Please do not use the recharging feature with standard batteries as damage to your unit can occur 14 Technical Parameters Technical Terms amp Concepts Total Magnification Total magnification of a microscope
10. the field men destroyed of view Stains dust or dirt have accumulated on the lens Clean the lens Unclear Image There is no cover slip on the slide Add a cover slip The objectives are designed for use with a 0 17mm cover slip so it is a requirement to use one for proper images The cover slip is not standard sized Replace the cover slip with the appropriate 0 17mm thickness slip The aperture is not open to an appropriate diam Adjust the aperture to have the light just larger than eter the size of the condenser Stain or dust has accumulated on the lensinthe Clean the lens with lens cleaner or a nonabraisive inlet ofthe head lint free cloth as well as spray with compressed air One side of the field The specimen slide is not fixed Secure the slide to the stage with clips of view is dark or the The nosepiece is not in the right position Turn the nosepiece until it clicks into place image moves while focusing The field of view is not The iris disc diaphragm is not big enough Rotate the iris disc diaphragm to allow more light bright enough to travel Stains dust or dirt has accumulated on the con Thoroughly clean tall lenses with lens cleaner or a denser objective eyepieces or base lens lint free nonabraisive cloth 16 Troubleshooting Common Issues Continued MECHANICAL ISSUES The objective touches the The cover slip is not standard sized Replace the cover slip with the appropriate 0 17mm thickness slip
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