PCR-258-Tip Kit Manual [110125]
Contents
1. cccccccc eee eeeeceeeeenseeeeeeesaeens 10 Section 4 Operational Guidelines 00000 II Section 5 Omni Tip Plastic Probes Dol o pecMca TONE asarus ierann a Na 13 5 2 Installing Omni Tips ccncscseccassciedaceneetmonstosaudeshjeneaneens 14 5 3 Chemical Resistance jgicvcanccsenoensntavasneatenereaiuadengeseeweeare I5 54 Autoclaving Omni VWiPSiescscsncstweeseetccenueswstunacuxpsecorweunoues I5 5 5 Cleaning the Omni Tip Adapter cc cece eee eee eee eee eens I5 Section 6 Motor Maintenance 6l Motor Drive UN iisncannascrcrcescwaccoatmeansoeeceuammesarameadianee 16 6 2 Brush Maintenance cc2cncsrcncscscicsesbstaerleciceensanicnslestisess 16 6 3 Motor Bearings cisc ccndensetevcasscuseenstoreeionstaneiaeetudewentecs 16 64 StOl ate ote ister sisi ica tise saie aR E 16 6 5 VV IIS Diaarinro teicher nemawnaties wan aoeet sas eaes 17 6 6 Replacement Parts ccctess acces aeceavicenveuless ceasenceieralareees 18 Section 7 Service Tcl PNSSISUANC aeara A EET E 19 7 2 Decontamination ccccccesesececsseseseveseueseceuesccssssossenss 19 Section 8 Troubleshooting c cece eee eee eee eeeees 19 5 SECTION OMNI TIP TISSUE HOMOGENIZING KITS 1 1 OMNI TIP KITS Omni s convenient Tissue Homogenizing Kits come with everything needed to homogenize tissue samples quickly and efficiently for successful RNA extraction Each kit includes an Omni TH Homogenizer with an adapt2. Processing Speed Omni Tip Homogenizing Kits include a handheld Omni TH Homogenizer with motor speeds up to 35 000rpm Operating at the top speed enhances processing efficiency of the generator probes both plastic and stainless steel supplied with the Omni Tip Kits Container Geometry and Size Round vessels encourage swirling Fluted or cornered vessels disrupt flow patterns allowing for more effective mixing processing Should further information or assistance be required for using Omni Tip Homogenizing Kits please contact Omni International Inc Toll free 800 776 443 Outside the US 1 770 421 0058 Fax 770 42 0206 E mail omni omni inc com Stainless Steel Rotor Stator Generator Probes 13 SECTION 5 OMNI TIP PLASTIC PROBES 5 1 SPECIFICATIONS 7mm Diameter Disposable Plastic Generator Probe Minimum Vessel Inside Diameter 8mm Applications Soft Tissue Omni Tips liquid liquid or soft tissue processing Hard Tissue Omni Tips frozen or fibrous tissue processing Processing Range 0 25 30ml Length 10mm The Omni Tips are disposable rotor stator generator probes constructed of durable plastic Although Omni Tips are designed specifically to be dis posed of after each use to eliminate sample cross contamination for many applications the probe can be cleaned sterilized or disinfected and reused by the procedures described below Soft Tissue Omni Tip Hard Tissue
3. OMNI P HOMOGENIZING KITS USER MANUAL OM MN 2 WARRANTY INFORMATION This manual is a guide for the use of the Omni Internationals Omni Tip Homogenizing Kits and accessories Data herein has been verified and validated It is believed adequate for the intended use of the instrument If the instrument or procedures are used for purposes over and above the capabilities specified herein confirmation of the validity and suitability should be obtained otherwise Omni International does not guarantee results and assumes no obligation or liability This publication is not a license to operate under or a recommendation to infringe upon any process patents Notes cautions and warnings within the text of this manual are used to emphasize important and critical instructions This Omni International product is warranted to be free from defects in material and workmanship for a period of ONE YEAR from the date of delivery Omni International will repair or replace and return free of charge any part which is returned to its factory within said period transportation prepaid by user and which is found upon inspection to have been defective in materials or workmanship For the first 90 days both parts and service are without charge For the balance of the period parts will be provided but service will be charged at established labor rates This warranty does not include normal wear from use it does not apply to any instrument or par
4. PCR 258 www omni inc com
5. applications where hands free operation is desired an optional stand assembly is available A post clamp assembly is included with the Omni TH for post mounting the unit to any comparable size ring stand Part Number Description S1000 Stand Assembly Kit Additional Omni Tip generator probes are available in boxes of 25 50 100 or 500 Omni Tips are I 10mm long and 7mm in diameter The adapter included in Omni s Tissue Homogenizing Kits is required for mounting Omni Tips to the Omni TH Homogenizer and is not disposable See Section 5 for more information about Omni Tips Part Number Description A 000SB Omni Tip Adapter 30750 25 Omni Tips 30750H 25 Hard Tissue Omni Tips 32750 50 Omni Tips 32750H 50 Hard Tissue Omni Tips 34750 100 Omni Tips 34750H 100 Hard Tissue Omni Tips 35750 500 Omni Tips 35750H 500 Hard Tissue Omni Tips 3M750 1000 Omni Tips 3M750H 1000 Hard Tissue Omni Tips Omni Tip Plastic Probes vi SECTION 2 OMNI TH TISSUE HOMOGENIZER The Omni TH Tissue Homogenizer is a variable speed handheld or post mounted homogenizer It combines a high speed high torque motor with a choice of autoclavable rotor stator generator probes of various Diameters or Omni Tip Plastic Probes 2 1 SPECIFICATIONS Motor Speed 5 000 35 000rpm Capacity 0 03 100ml P y Height motor only 22 9cm Vin Sf Omni m N Tissue Homogenizer Weight motor only 466g 16
6. 4072 Electrical Requirements 15V 60Hz or 220V 50Hz Standards Approval Compliance 220V CE Certified 2 2 PARTS AND ACCESSORIES Prior to operation please remove all parts from the shipping container and inspect for damaged or missing parts If any parts are found to be damaged or missing please contact Omni International at 800 776 443 1 The Omni TH Tissue Homogenizer consists of the following Description Part Number Quantity Motor Drive Unit 1 15V TH 01 220V TH 02 Post Clamp Assembly 2 118 Tool Kit TOO Instruction Manual H258 TH A stand assembly base plate 24 post cross rod and post clamp PN S1000 is available for use with the Omni TH Tissue Homogenizer but is not supplied with the instrument SECTION 2 OMNI TH TISSUE HOMOGENIZER cont 2 3 STAND MOUNTING THE OMNI TH Optional To mount the Omni TH Tissue Homogenizer to a stand assembly refer to the figure and follow these steps Secure the 24 post by twisting it into the base plate until securely tightened Motor Brushes 2 Loosen the locking knob and slide the Ng included post clamp assembly down Post Clamp i i A bl over the end of the post until the Motor Drive ssembly ma clamp is at the desired height and lock nil in place Thumb Screw 3 Slide the TH motor into the opening Locking Knob on the end of the post clamp assembly and push it down firmly The unit will 24 Post
7. H Tissue Homogenizer motor is equipped with sealed ball bearings Under normal use they require no additional lubrication 6 4 STORAGE The unique design of the Omni TH Tissue Homogenizer allows the handheld unit to stand vertically on a bench top or shelf so that it can be stored in less than six square inches of space WARNING Keep all housings in place and in working order WARNING Remove all tools from the generator probe before turning the motor on WARNING DO NOT use the motor in a dangerous environment WARNING Disconnect the motor before servicing and when changing the generator probe 17 SECTION 6 MOTOR MAINTENANCE WARNING DO NOT modify the plug or cord that is provided If the plug will not fit the outlet have the proper outlet installed by a qualified electrician WARNING Reduce the risk of unintentional starting make sure the power switch is in the OFF position before plugging in the motor WARNING Damaged or worn power cords should be repaired or replaced immediately by a qualified electrician WARNING Improper connection of the equipment can result in a risk of electric shock 6 5 WIRING DIAGRAM Slide Switch Assembly On Off Thermal Cutout Switch Speed VAC Motor Adjustment 18 SECTION 6 MOTOR MAINTENANCE A 6 6 REPLACEMENT PARTS Table of Replacement Parts for the Omni TH Tissue Homogenizer Description 115V 220V Aluminum Washer 2 113 12 113 2 Wav
8. Omni Tip NOTE Omni Tip plastic probes are not factory presterilized or disinfected If this is a requirement please use one of the recommended methods described below Recommended Not Recommended Sterilization Techniques Autoclave up to 250 F UV Irradiation Ethylene Oxide Formaldehyde Gluteralhyde 2 Gamma irradiation up to 5 MRAD Biological Disinfection Alcohol 70 Phenolic Derivatives Hypochlorite 5 Extended Exposure to chlorine bleach Guanidine Formaldehyde 40 Dichloromethane Radioactive Decontamination Radiacwash Count off Water Ethanol SDS 14 SECTION 5 OMNI TIP PLASTIC PROBES WARNING Safety glasses are recommended when using Omni Tip plastic probes It is possible for plastics to shatter or break after exposure to certain chemicals or after repeated autoclaving CAUTION Large solid particles such as frozen tissue may cause damage to the standard Omni Tip generator probes Use the Hard Tissue Omni Tips for those applications 5 2 INSTALLING OMNI TIPS See Section 2 2 CAUTION Omni Tip plastic probes should not be immersed above the cone leading to the adapter during processing since liquid may be drawn up the shaft and contaminate the coupling 5 3 CHEMCIAL RESISTANCE Omni Tips generator probes are made of polycarbonate and an amorphous thermoplastic polythermide The plastics used in Omni Tips have good chemical resistan
9. TH to an appropriate power outlet Speed of the Omni TH varies between 5 000 and 35 000 rpm and is set by means of the blue slider switch The power switch located above the blue slider switch turns the motor on and off WARNING Reduce the risk of unintentional starting make sure that the power switch is in the OFF position prior to plugging in the unit Insert the generator probe into the Omni TH motor see above The depth of the generator probe in the sample vessel can significantly affect flow patterns within the vessel This also affects processing efficiency As a rule of thumb the generator probe usually operates most efficiently at a depth of 1 3 to 1 2 of the liquid height Heavy sediments may require deeper immersion and this processing depth can be optimized by observing flow patterns and related processing results While processing liquid can circulate through the two holes at the bottom of the generator probe When using a stainless steel probe the top hole should not be immersed in the sample Blocking the upper hole could result in liquid being drawn into the lower motor bearing Operating at the top speed of 35 000rpm enhances the processing efficiency of the rotor stator generator probe For most samples three to four short bursts at top speed should be sufficient for complete homogenization of the sample WARNING The Omni TH Tissue Homogenizer has an internal speed control An external speed control should ne
10. ce to weak acids chlorides hypochlorides and many other chemicals allowing for reuse However phenol or guanidine based reagents commonly used in PCR studies are not recommended for extended use with Omni Tips Exposure to these chemicals shortens the life of Omni Tips and usually results in disposal after each use Therefore reuse is not guaranteed in such circumstances If any cracking or brittleness is detected in an Omni Tips generator probe it is recommended that it be discarded Due to the many different chemicals and environments in which processing takes place it is the responsibility of the user to set sterilization guidelines and protocols to optimize the life and performance of their Omni Tips generator probes 15 SECTION 5 OMNI TIP PLASTIC PROBES 5 4 AUTOCLAVING OMNI TIPS Omni Tips generator probes can be autoclaved up to seven times and re tain their mechanical properties Due to different combinations of chemicals and environments it is suggested that the autoclaving recommendations be followed as a guideline only and the user should decide how to best alter the procedure for their specific application The tube and shaft of Omni Tips can be autoclaved at up to a maximum of 250 F for 45 minutes This includes a 15 minute ramp up 15 minute hold and a 15 minute ramp down of the temperature cycle If the tube or shaft begins to warp as a result of autoclaving discard and lower the
11. cycle time or temperature level accordingly for future runs 5 5 CLEANING THE OMNI TIP ADAPTER The Omni Tip adapter is constructed of stainless steel with steel and Alu minum rotating components For general cleaning it is recommended that the adapter be wiped down with a lint free cloth and an alcohol solution Adequate molecular contamination cleaning can also be accomplished by scrubbing with soap and hot water followed by brief rinsing with 1 SDS and then by distilled water which has been treated with diethylpyrocarbonate DEPC Autoclaving baking or submersion in cleaning agents is not recommended this will shorten the life of the adapter housing and bearings Omni Tip Adapter 16 SECTION 6 MOTOR MAINTENANCE 6 1 MOTOR DRIVE UNIT The Motor drive housing should be wiped off after use especially when concentrated and potentially damaging liquids are used during processing Never use solvents to clean unit or accessories 6 2 BRUSH MAINTENANCE If the performance of your Omni TH Tissue Homogenizer begins to deteriorate in any of the following ways e The operations speed begins to decline e The unit stalls intermittently e The unit stops working all together It probably requires a new set of brushes The average brush life for the Omni TH Tissue Homogenizer is 80 100 hours For service or brush replacement please contact Omni International at 800 776 443 6 3 MOTOR BEARINGS The Omni T
12. e Spring 00 544 00 544 3 Set Screws 00 695 00 695 4 Coupling 2 112 2 112 5 Bearing Insulator 12 114 12 114 6 Armature Assembly THII52_B TH2252 7 Field Assembly 20052_B 20252 8 Speed Switch Assembly 20010 B 22010 9 Thermal Sensor 00 122 00 122 10 TH Bearing Support THII57_B THII57_B II Power Cord THIISI_B TH2251_B 2 Housing Assembly THII5 _B THII5I_ B 13 On Off Switch 00 115 00 115 14 Armature Brush pack of 2 62810 62810 I5 Brush Caps pack of 2 2081 2081 19 SECTION 7 SERVICE 7 1 ASSISTANCE Should this product ever require service please contact Omni International at 800 776 443 7 2 DECONTAMINATION Should an instrument or component that has been used with radioactive or pathogenic material require factory or field service comply with the following procedure to ensure the safety of service personnel e Clean the parts to be serviced of all encrusted material and decontaminate them There must be no radioactivity detectable by survey equipment e Obtain a Decontamination Certificate from Omni International Complete the certificate and attach to the instrument or parts being returned If no Decontamination Certificate is attached and a potential radioactive or biological hazard is detected or suspected by Omni International the equipment will not be serviced until proper decontamination and certification is complete The sender will be contacted for instructions as to the disposition of the equipm
13. ent Disposition costs will be borne by the sender WARNING It is a violation of federal law to transport biologically hazardous or radioactive materials without proper packaging labeling and appropriate warnings SECTION 8 TROUBLESHOOTING Do not attempt to service the Omni TH in a manner other than those discussed in this manual For any issue that is unsuccessfully corrected using this guide please contact your authorized dealer or call Omni International technical assistance at 800 776 4431 or 770 421 0058 PROBLEM CORRECTIVE ACTION The Omni TH is plugged in and turned on Check power cord connectors but is not functioning Check wall socket for power to the outlet Motor is turned ON and makes a makes a Check that the brushes are not worn buzzing sound but is not working and are correctly installed Replace if necessary see Section 3 2 Motor unit operating speed declines stalls Check that the brushes are not worn intermittently or stops completely and are correctly installed Replace if necessary see Section 3 2 Teflon bearing wears quickly Fluid level may be too low in the tube Immerse the probe deeper into the fluid Excessive splashing in sample tube Fluid level too low for tube size 20 k OMNI 935 C Cobb Place Blyd Toll Free 1 800 776 4431 Kennesaw GA 30144 Phone 770 42 1 0058 Email omni omni inc com FAX 770 421 0206 Patents Pending Rev 01 25 11 rb P N
14. er for mounting Omni Tips generator probes see Section 5 for more information A choice of stainless steel rotor stator generator probes is also available see Section 6 for more information Omni s Tissue Homogenizing Kits are designed for situations where molecular contamination between samples cannot be tolerated Stainless steel generator probes are available for efficient processing of frozen and or fibrous tissues Omni Tips are ideal when molecular contamination is of the utmost concern and are most beneficial when working with soft unfrozen tissues It is important that the diameter and length of the selected generator probe will fit securely within the vessel being used and the generator probe plastic or stainless steel is appropriate for the application a es aie e a te 7 A a AA y Omni Tip Kit with 7mm Stainless Steel Generator Probe Our Applications database can help you find the best solution quickly Our constantly expanding database consists of carefully reviewed customer published applications sorted into useful categories complete with product recommendations to help you find the right laboratory homogenizer for any sample type If you can t find an answer in the database call us and we ll help www omni inc com applications 6 SECTION OMNI TIP TISSUE HOMOGENIZING KITS 1 2 OPTIONAL ACCESSORIES Omni Tip Homogenizing Kits are supplied with a handheld Omni TH Homogenizer For
15. gt lock in place Base Pl 4 To remove the TH press the black ans button on the back of the opening of the post clamp assembly and lift the motor from the cradle 2 1 INSTALLING ROTOR STATOR GENERATOR PROBES Stainless steel rotor stator generator probes are easily installed to the Omni TH motor by means of a quarter turn bayonet mount Simply push the generator probe into the motor housing as far as possible turn clockwise and release Turn the thumb screw clockwise to tighten Remove the blue protective cap from the tip of the generator probe and the Omni TH is ready to operate WARNING The tip of the saw tooth generator probe is sharp CAUTION When using glass filled Teflon lower bearings do not operate the unit for extended periods of time without immersing the bottom of the generator probe in liquid or the sample being processed in order to avoid premature failure of the lower bearing SECTION 3 OMNI TH OPERATION 3 2 OMNI TIP PLASTIC PROBES An Omni Tip Adapter Al O00SB is required to use Omni Tip Plastic Probes with the Omni TH Homogenizer First insert the smaller end of the Omni Tip Adapter into the Omni TH as far as possible turn clockwise and release Next insert an Omni Tip Plastic Probe into the Omni Tip Adapter as far as possible turn clockwise and release The TH is now ready to operate Omni Tips can be disposed of after use 3 3 OMNI TH OPERATION Connect the Omni
16. l resistance compatibility Disposal may be required when used in conjunction with these reagents Stainless steel generator probes are best for difficult to process tissues and for situations where sample to sample contamination is not a concern Stainless steel generator probes are equipped with a Teflon bearing Omni s stainless steel generator probes can be quickly disassembled for thorough cleaning and bearing maintenance Omni s stainless steel generator probes are available in many configurations tailored for specific sample types Flat bottom generator probes are recommended for liquid or soft tissues while saw tooth and extended blades are best for processing fibrous tissues For situations where preprocessing or cutting the tissue prior to homogenization is inconvenient wide windows allow larger pieces of sample to effectively pass through the processing head where shearing occurs 12 SECTION 4 OPERATIONAL GUIDELINES Regardless of which type of generator probe is being used the following factors can affect processing efficiency Size and Type of Material Being Processed Solid particles in any dimension should be no more than half the diameter of the rotor stator generator probe for optimal processing This is extremely important when using the Omni Tip disposable generator probes Stainless steel generator probes with extended blades and or wide windows are available for processing large solid samples
17. ng efficiency can be affected by e Amount of material processed vs size and speed of the generator probe e Container geometry and size round vessels encourage swirling while fluted or cornered vessels disrupt flow patters for more effective mixing processing e Processing speed vs optimal speed e Size and type of material and flow characteristics material particles must be small enough to be carried into the generator head for optimal processing 11 SECTION 4 OPERATIONAL GUIDELINES 4 1 OPERATIONAL GUIDELINES Rotor stator homogenization provides highly efficient cellular lysis and membrane disruption while reducing the high molecular weight of cellular components Omni Tip Tissue Homogenizing Kits allow rapid and complete disruption of cell lysates to expose intracellular contents and ensure increased yields A choice of rotor stator generator probes in plastic or stainless steel process a wide range of sample preparation applications Non sterile plastic Omni Tips generator probes are ideal for situations where molecular contamination between samples cannot be tolerated Omni Tips were designed to be disposed of after each use eliminating cross contamination risks along with the time and effort normally required for cleaning While Omni Tips can be autoclaved up to seven times for repeated use they are not recommended for extended use with phenol or guanidine based reagents see Section 5 3 for chemica
18. t to modify any part of the Omni TH If you experience problems with or have questions about your Omni TH contact your authorized dealer or call Omni International at 800 776 4431 or 770 42 I 0058 DANGER DO NOT allow the machine to be submerged in any liquid DO NOT use in any setting other than an indoor laboratory DO NOT plug power cord into an incorrect outlet WARNING To reduce the risk of burns electrocution fire or injury Use this product only for its intended purpose as described in this booklet DO NOT use attachments not recommended by the manufacturer DO NOT operate the product if it is damaged in any way Keep this product away from heated surfaces TABLE OF CONTENTS Warranty Information cc cece cece cece eee eee eeenees 2 Section Omni Tip Tissue Homogenizing Kit MES WAR EE E E sins T T E 5 2 Optional ACCESSOriES carnai EE a 6 Section 2 Omni TH Tissue Homogenizer 2 SPECINCALIONS nisseccenctssaceradeseheesintescnsere E EEE Ei 7 2 2 Parts and Accessories ssesssssssesssssssessssresesseseeee 7 2 3 Stand Mounting the Omni TH ce ee eeee cece eeteee ee ees 8 Section 3 Omni TH Operation 3 1 Installing Rotor Stator Generator Probes ccceeeee eee 8 3 2 Omni Tip Plastic PrODCS saccccsincindicvescedesscnaneeceevncenseeses 9 33 Omni TH Ope raulon ais icscacascscoessacdeusi dre ieasasiessacerensen 9 3 4 Theory of Operation
19. ts which have been altered by anyone other than an employee of Omni International nor to any instrument which has been damaged through accident negligence failure to follow operating instructions the use of electric currents or circuits other than those specified on the plate affixed to the instrument misuse or abuse Omni International reserves the right to change alter modify or improve any of its instruments without any obligation whatever to make corresponding changes to any instrument previously sold or shipped THE FORGOING OBLIGATION IS IN LIEU OF ALL OBLIGATIONS AND LIABILITIES INCLUDING NEGLIGENCE AND ALL WARRANTIES OF MERCHANTABILITY OR OTHERWISE EXPRESSED OR IMPLIED IN FACT OR BY LAW AND STATE OUR ENTIRE AND EXCLUSIVE LIABILITY AND BUYERS EXCLUSIVE REMEDY FOR ANY CLAIM OF DAMAGES IN CONNECTION WITH THE SALE OR FURNISHING OF GOODS OR PARTS THEIR DESIGN SUITABILITY FOR USE INSTALLATION OR OPERATION OMNI INTERNATIONAL WILL IN NO EVENT BE LIABLE FOR ANY SPECIAL OR CONSEQUENTIAL DAMAGES WHATSOEVER AND THEIR LIABILITY UNDER NO CIRCUMSTANCES WILL EXCEED THE CONTRACT PRICE FOR THE GOODS FOR WHICH LIABILITY IS CLAIMED oS IMPORTANT SAFEGUARDS READ ALL INSTRUCTIONS BEFORE USING SAVE THIS OWNER S MANUAL The Omni TH has been engineered for economical functionality as well as safety however basic safety precautions and common sense must always be demonstrated when using any electrical product Do not attemp
20. ver be used with this instrument 10 SECTION 3 OMNI TH OPERATION NOTE For optimal sample recovery during processing reduce the motor speed and completely remove the generator probe from the sample Then turn off the motor drive unit WARNING DO NOT process pathogenic material in an open container since aerosols created during normal processing could be inhaled by the operator Please call for assistance in processing pathogens or other material which require sealed enclosures 3 4 THEORY OF OPERATION The rotor shaft is coupled directly to the drive motor via the drive pin When attached to the homogenizer motor the rotor shaft can spin up to 35 000rpm This assembly makes up the rotor portion of the rotor stator generator probe The tube collar assembly is attached to the motor housing but does not spin This is the stator portion of the rotor stator generator probe As the rotor knife spins within the tube and collar assembly it creates a pumping action pulling the sample into the open end of the generator probe and forcing the sample out through the windows in the tube The interaction of the rotor knife with these windows sets up a shearing action reducing the particle size of the sample The speed differential between the rapidly moving portion and the relatively stationary portion of the sample sets up a second force called cavitation which pulls the sample apart further reducing the particle size The processi
Download Pdf Manuals
Related Search