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1. Request to set or query auto fill setting Set AF ON turns Auto Fill feature on Note The syringe volume is also needed for auto fill to operate See SYR command AF OFF turns Auto Fill function off Command will not be accepted if the pump is running and a Not Applicable response will be given Query AF If Auto Fill function is ON response will be ON If Auto Fill function if OFF response will be OFF 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Pump Chain Commands SYR lt float gt Request to set or query syringe volume setting for auto fill Used in conjunction with Auto Fill feature See AF command Set syringe volume SYR volume Volume is of format f f f f f f Units are ML If volume is accepted and valid volume will become the new syringe Auto Fill volume Volume will not be accepted if the pump is running and a Not Applicable response will be given If the volume is invalid an Out Of Range response will be given Query syringe volume SYR Response is of the following format space space f f f f f f Request to read the TTL logic level of the specified pin on the external 9 pin D SUB connector Valid pin numbers for input are 6 7 8 and 9 If the pin specified is valid and if the pin level is high response will be ON If the pin level is low response will be OFF If the pin specified is invalid An Out Of Range response will be given OUT d lt ON OF
2. Request to set or query target volume setting Set target volume TGT volume Volume is of format f f f f f f Units are ML If volume is accepted and valid volume becomes new target vol ume Volume will not be accepted if the pump is running and a Not Applicable response will be given If the volume is invalid an Out Of Range response will be given Query volume TGT Response is of the following format space space f f f f f f Units are ML E Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Pump Chain Commands MOD PMP VOL PGM Request to set or query pumping mode Set MOD PMP Puts pump in Pump Mode MOD VOL Puts pump in Volume Mode MOD PGM Puts pump in Program Mode Command will not be accepted if the pump is running and a Not Applicable response will be given Query MOD If mode is PUMP response will be PUMP If mode is VOLUME response will be VOLUME If mode is PROGRAM response will be PRGRAM DIR INF REF REV Request to set or query pumping direction Set DIR INF sets pumping direction to infusion DIR REF sets pumping direction to refill DIR REV reverses current pumping direction Command will not be accepted if the pump is running in volume or pro gram modes and a NotApplicable response will be given Query DIR If pump direction is infusion response will be INFUSE If pump direction is refill response will be REFILL AF ON OFF
3. begin after an external or keyboard run command Otherwise the sequence will pause for specified time interval Sequence is repeated the specified number of times Travel direction is as specified Data Specified Strategy 1 Flow rate Strategy 2 Flow rate Volume Time Interval 00 00 00 Time Target volume Target volume Time interval Number of repetitions Number of repetitions Pumping direction Pumping direction Event Program Events A program event is an external event defined as a high to low tran sition on TTL pin 9 Within a program a one time event trigger can be set which watch es for and acts upon the external event The triggered event causes an immediate con tinuation of the program at the specified sequence and the operation of the pump will be according to this sequence Data Specified Go To sequence number Go To Causes the program to immediately continue operation at the sequence specified Data Specified Go To sequence number Pause Pump stops for specified time then continues with next programmed sequence Current program rate set to 0 with no change in units Data Specified Time interval Pump Runs the pump continuously at the specified flow rate without any pumping target This mode can provide a background flow rate while waiting for an external event to trigger a new sequence specified by the EVENT operation Data Specified Flow rate Pumping direction 4 Harvard Apparatus PHD 200
4. the pump stops or the direction of pumping changes except before and after Auto Fill of the syringe If the pump stops due to the pump stalling an asterisk will be appended to the volume printed In addition the entered pump program can be listed on the printer by pressing the PROGRAM key for about one second with the pump stopped TTL Devices The pump does not need to be configured to attach aTTL device Simply plug the device into the 9 pin connector on the rear of the pump See Appendix E for wiring specifications Foot Switch or Relay Used to start and stop the pump Pressing the foot switch performs the same function as pressing the RUN STOP key on the keyboard The foot switch connector allows remote or automated operation of the pump Timer Opening the timer input starts the pump Closing the timer input stops the pump The timer input allows for an externally controlled pumping interval Pumping Direction Not applicable for Infusion model Sets the direction of pumping Opening the directional input sets the pump to infuse Closing the directional input sets the pump to refill The pumping direction input is recognized only in the situations that the INFUSE REFILL key would be recognized i e when the pump is stopped or running in the Pump Mode Valve Control Not applicable for Infusion model The valve control output is an indicator of the direction of pump travel When the out put is high the pump is set to Refill A
5. 232 Devices acer ctesinsarastiemermesustsnsnicacsmesnendc 25 Configuring Pump for RS 232 Devices 25 P mp Chain Sceacercne cote seerceaseerescdenseieapecoetrccxecceeee 25 oC sn ee ne eee ne eee een eee Pr eee 26 O E E A 26 TTL Devices sissies scececiersccreccaseceusnccessesiaeveeeceneens 26 Foot Switch or Relay de pacccestercnctnsesanesetenceensunece 26 TIMET eseina E EE EEEE TEARRE 26 Pumping Direction vesceccececesecevenccesseensecttandeesdacens 26 Valve Control cccccccccecececeeecccecceccaeeececeeseeees 26 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Table of Contents 10 Programming Tutorial ccceseeeeeeeeeeeeeees 27 Multiple Infusion Example ceeeees 27 Ramping Up Infusion Rate Example 28 Multiple Dispensing Example eee 29 Periodic Dispense Loop Example 30 Comb of Infusion and Withdraw Profiles Ex 31 ISSO EVGiIS rrinin s eia 32 11 Pump Chain Commands ceeseee 34 45 M del 22 ROO CON sisareni 35 Model 44 Prot CO l eeren 37 A Table of Syringe Diameters 46 B Stainless Steel Syringes eee 47 C Nominal Min Max Flow Rates 48 D PHD 2000 to PC Connection 49 E TTL Connector Specifications 49 F RS 232 Specifications cccccce 50 G Maintenance and Troubleshooting 51 H Accessories and
6. ASCII 13 line feed ASCII 10 ffffff ddddd d dd dd any string of ASCII characters Command Formats and Meanings Command Format lt cr gt pump address lt cr gt optional pump address command lt cr gt Meaning Stops all pumps All pumps on the pump chain interpret this as a stop command Request for prompt The pump with the indicated address responds with its prompt Send a command to a pump The pump with the indicated address executes the command then responds with its prompt The optional pump address if not specified will default to pump address 0 After each command is received and executed the pump acknowledges the command with aprompt Preceding the prompt may be some additional text responses The addi tional text will be one or more lines of ASCII text each preceded by a line feed and terminated by a carriage return lt f gt lt text gt lt cr gt A prompt is a string of ASCII characters sent by a pump indicating the pumps address and its present state lt f gt 1 or 2 digit address prompt character Prompt Characters SRE OS AN OTE Meaning Pump stopped Pump infusing Pump refilling Pause interval pump stopped Pumping interrupted pump stopped Dispense trigger wait pump stopped 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Pump Chain Commands Pump Commands and Responses RUN Starts pumping according to the present setting of th
7. Series User s Manual Operation Because of the wide range of functions that the PHD 2000 is capable of performing cer tain information about your application must be entered into the pump At minimum the pump needs to know the Diameter of your syringe the Infusion Rate and Direction of travel Direction of travel Infuse Refill is not applicable to the Infusion model This is the only information needed to run the pump in the Pump Mode If not specified the Refill Rate Refill Rate is not applicable to the Infusion model will default to the Infuse Rate The pump will need additional information to utilize its more advanced features See Section 5 User Interface for general information on data entry SYRINGE LOADING Standard with a 2 Syringe Holder The Pusher Block 2 and Syringe Holder Block 3 are fitted with movable retaining brack ets 4 and 5 Available as an option for the PHD 2000 Infusion model as an Anti siphon kit Catalog 70 2015 which hold the syringe barrel and plunger firmly when refilling When loading the syringe into the pump it is necessary to adjust these retaining brackets and the Adjustable Stops 8 amp 9 The Pusher Block 2 is fitted with Release Tabs 1 to release the drive nut from the leadscrew so that the Pusher Block 2 can be moved freely on the leadscrew to facilitate loading the syringe Refer to Figure 5 1 Squeeze the Release Tabs 1 towards each other and move the Pusher Block 2 to the left al
8. mm 5 12 34 10 14 55 20 19 86 30 23 20 50 27 60 Size SGE Scientific Glass Engineering Size Diameter 25 ul 0 73 mm 50 1 03 100 1 46 250 2 30 500 3 26 1 0 ml 4 61 mm 7 28 10 30 Hamilton M icroliter Series Gastight Size 0 5 ul Diameter 0 103 mm 0 1457 0 206 0 3257 0 460 0 729 1 031 1 46 2 3 3 26 4 61 mm 7 28 10 3 14 57 23 0 Becton Dickinson Glass All Types Size Diameter 0 5 ul 4 64 mm 1 4 64 2 5 8 66 5 11 86 10 14 34 20 19 13 30 22 70 50 28 60 100 34 90 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Appendix B Stainless Steel Syringes 200 ml Harvard High Pressure Stainles Harvard High Pressure Stainless Steel Syringes Harvard now offers a completely new line of Stainless Steel Syringes intended for high pressure applications with good resistance to most aggressive liquids Wetted parts are 316 stainless steel or Viton Syringes are available in 20 50 100 and 200 ml sizes with removable replace able tips Genuine SWAGELOK syringe to tube fittings are available in 1 16 1 8 and 1 4 inch sizes A luer lock end fitting is also available All tips are interchangeable with all syringes 20 to 200 ml in the series Both syringe barrel end plungers are 316 stainless steel A Viton O ring seal between top and end of the barrel insures against leakage Syringes are guaranteed to be leak free for pressures up to 700 psi All
9. patients 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Warranty and Repair Information GENERAL SAFETY SUMMARY Please read the following safety precautions to ensure proper use of your syringe pump To avoid potential hazards and product damage use this product only as instructed in this manual To Prevent Hazard or Injury Use Proper Line Cord Use only the specified line cord for this product and make sure line cord is certified for country of use Ground the Product This product is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or output terminals of the product ensure that the product is properly grounded Make Proper Connections Make sure all connections are made properly and securely Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections Use Proper Fuse Use only specified fuses with product Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection Place Product in Proper Environment Review the operating manual for guidelines for proper operating environments Observe all Warning Labels on Product Read all labels o
10. s output pin level SEQ lt n gt OUT lt ON OFF gt Request for version of pumps embedded software Pump Chain Error Messages Error messages are in the format lt f gt space space lt message gt lt cr gt Where lt message gt is one of the following Syntax error in areceived command NA Command not applicable at this time OOR Control data is out of the operating range of the pump Note If assistance is needed in programming pump control via RS232 call Harvard Apparatus Customer Service Support at 1 800 272 2775 T Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Appendix A Syringe Diameters in mm Stainless Steel Size Diameter 8 cc 20 50 100 9 525 mm 19 130 28 600 34 900 Becton Dickinson Plastic Plasti pak Diameter 4 78 mm 8 66 12 06 14 50 19 13 21 70 Air Tite All Plastic Size Diameter 2 5 CC 5 10 20 9 60 mm 12 45 15 90 20 05 22 50 Unimetrics Series 4000 amp Size 5000 Diameter 10 ul 25 50 100 250 500 1000 0 460 mm 0 729 1 031 1 460 2 300 3 260 4 610 Terumo Diameter 3 cc 8 95 mm 5 13 00 10 15 80 20 20 15 23 10 Size Sherwood M onoject Plastic Diameter 4 65 mm 8 94 12 70 15 90 20 40 23 80 26 60 Popper amp Sons Inc Perfektun Glass _Size ___ Diameter 0 25cc 3 45 mm 0 5 3 45 4 50 8 92 8 99 11 70 14 70 19 58 22 70 29 00 Diameter 2cc 9 12
11. syringes are supplied with inside diam eter dimensions for use with Harvard microprocessor controlled pumps and rate charts for use with older classic pumps Replacement Viton O Rings are available as are the more chemically resistant Chemraz O Rings Harvard High Pressure Stainless Steel Syringes With SWAGELOK Diameter Syringe Size 1 16 inch 1 8 inch 20 ml 55 4199 55 4205 50 ml 55 4200 55 4206 100 ml 55 4201 55 4207 200 ml 55 4202 55 4208 Harvard High Pressure 8 ml Stainless Steel Syringe This syringe has been designed to utilize the high forces avai pumps to produce p psi The syringe is co able in our syringe ressures up to 1500 nstructed entirely of 16 stainless steel with two Chemraz O Ring seals two Tef on ORing seals and 1 6 mm 1 16 in SWAGELOK end When used in the 70 2200 or 70 2201 Harvard PHD 4400 pressures in excess of 1500 psi are achieved for chromatography and process control applications Specifications Volume 8 ml Dimensions Length of Barrel 16 5 cm 6 1 2 in Plunger Excursion 11 4 cm 4 1 2 in oD 1 6 cm 5 8 in ID 0 95 cm 3 8 in Max Test Pressure 4000 p s i Working Pressure 1500 p s i Cat No Product 55 3369 Harvard High Pressure 8 ml Stainless Steel Syringe Replacement Parts 5013 089 5013 090 5013 091 5013 092 5013 087 72 2472 72 2473 72 2474 72 2475 72 2476 72 2477 Stainless Steel Chemraz O Ring 20 ml Chemraz O Ring 50 ml
12. the Refill Rate can be changed If the new rate is valid it will take effect when the ENTER key is pressed Refill Rate is not applicable to the Infusion model The minimum and maximum rates permitted vary depending on the diameter of the syringe If an OUT OF RANGE message is displayed when entering a rate try using a different syringe for your application Target Volume The Tagret Volume is the volume that you desire to deliver at the set Infuse or Refill Rate The pump must be set for this Volume mode for this feature to work When enter ing the Target Volume enter the volume desired in mls milliliters and press the ENTER key Auto Fill only applicable to programmable model Use AUTO FILL key to toggle between Auto Fill ON and OFF If Auto Fill is set to ON the pump will next request the volume of the syringe in milliliters The volume of the syringe is used as the refill volume of the syringe This volume can be set to a volume smaller than the syringe if desired When set to ON the syringe is assumed to be empty Auto Fill continuously monitors the volume of the syringe according to the volume pumped When the pump deter mines that the syringe is empty the operation in progress is suspended and Auto Fill is activated The pumping direction is then reversed and the pump runs at the refill rate During the Auto Fill operation the display will indicate the volume of liquid in the syringe When the vol
13. used with this pump They can withstand high pressure applications and Rates listed are for single syringe provide years of service Flow Rates for PHD 2000 HVP High Volume and Pressure Syringe Size Minimum Maximum 20 ml 1 5 yl hr 20 ml min 50 ml 3 4 ul hr 46 ml min 100 ml 5 0 pl hr 68 ml min 200 ml 8 2 ul hr 112 ml min 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Appendix D PHD 2000 to PC Connection oe Cp tarnight ool _ trite or i a L or fer Maa Heime B PA ML 112 Cth aa 156 LADON PN RAA DIEF Figure 13 PHD 2000 to PC Connection Appendix E TTL Connector Specs TTL Connections are via a 9 pin Tam ii D sub type connector All inputs are internally pulled up to a logic high EE a Pin Assignments age hgh Seem ai 1 Vss Ground Reference logic low Falling edge starts stops pump 2 Valve Control Output Low Infusing MEFE S High Refilling 3 Running Output Indicator ais Low Pumped stopped ier High Pump running Figure 15 TTL Connector Specifications 4 Programmable Output Falling edge sets pump to refill 5 Logic High Reference 5V 400 pA 9 Event Input 6 Foot Switch Input Falling edge triggers program 7 Timer Input event Rising edge starts pump Logic Low 0V 0 5V Falling edge stops pump Max 2 mA current sink 8 Directional Control Input Logic High 2V 5V Rising edge sets pump to infuse Max 400 pA current sour
14. 0 Syringe Pump Series User s Manual Program Mode TTL Out ProgrammableTTL Pin TTL output Pin 4 can be set to aHIGH or LOW level from with in aprogram Data Specified TTL pin level Restart Immediately restart program from the first sequence Data Specified None Stop Stops pump and the program terminates Data Specified None Program Printout If a printer is attached and the pump is stopped a program listing can be obtained by pressing the PROGRAM key for about one second PRINTING PROGRAM will be dis played while data is being sent Program Run Time Error Messages If while running a program an operation is requested that cannot be performed the pump will stop and an error message will be displayed Error messages will be dis played with the following format Program N SEQ n message Where N is the Program number and n is the sequence number when an error was detected and message is the indicated error as follows INFINITE LOOP A GOTO sequence cannot specify the current sequence INVALID GO TO The target of the GO TO specified an invalid sequence number RATE UNDERFLOW A decrement sequence decremented a rate to less than or equal to 0 RATE OVERFLOW An increment sequence caused an arithmetic overflow OUT OF RANGE Specified or calculated rate is beyond the pumps capabilities with the specified syringe VOL TGT ERROR A sequence with a volume target cannot follow a sequence wi
15. 0 VAC 220 260 VAC Dimensions HxWxD 15 9 x 22 8 x 27 9cm 6 3 x 9 x 11in Syringe Holder HxWxD N A Remote Cable Length N A Weight 4 5 kg 10 Ib From 8 000 to 128 000 27 3 sec step 416 7 usec step 0 09 um min 71 210 mm min 115 230 VAC 50 60 Hz External voltage selection 65 W 0 5A fuse 95 130 VAC 220 260 VAC 9 5 x 27 9 x 22 9 cm 3 75 x 11 x9 in 22 9 x 43 2 x 30 5 cm 9x 17x 12 in 152 cm 60 in approx 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Ordering Information Harvard PHD 2000 Syringe Pump Series Pump Versions PHD 2000 Infusion Only PHD 2000 Infuse Withdraw PHD 2000 Programmable Infuse Withdraw PHD 2000 Infusion Only with 6 10 Multirack PHD 2000 Infusion Only with 4 x 140 Multirack PHD 2000 Infusion Only with Microliter Rack PHD 2000 Infuse Withdraw with 6 10 Multirack PHD 2000 Infuse Withdraw w 4 x 140 Multirack PHD 2000 Infuse Withdraw with Microliter Rack PHD 2000 Programmable with 6 10 Multirack PHD 2000 Programmable with 4 x 140 Multirack PHD 2000 Programmable with Microliter Rack Standard 29 Ibs Force Regular Remote 70 2000 70 2100 70 2001 70 2101 70 2002 70 2102 70 2003 70 2103 70 2004 70 2104 70 2005 70 2105 70 2006 70 2106 70 2007 70 2107 70 2008 70 2108 70 2009 70 2109 70 2010 70 2110 70 2011 70 2111 Harvard PHD 2000 Push Pull Syringe Pump Series Standard 29 Ibs Force Pump Versions PHD 2000 Infusion Withdra
16. ARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practi cal enclose a sample or drawing We offer a complete reconditioning service Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency AN CAUTION Not for clinical use on human
17. Chemraz O Ring 100 ml Chemraz O Ring 200 ml Chemraz Tip Seal O Ring all sizes Replacement Viton O Ring 20 ml 10 Replacement Viton O Ring 50 ml 10 Replacement Viton O Ring 100 ml 10 Replacement Viton O Ring 200 ml 10 Replacement Viton Tip Seal O Ring 10 Plunger Button to Adapt Syringe for Use with PHD 2000 HVP with Triple S Clamp With Luer Lock Replacement Tips Furnished with Sealing O Ring 55 4220 55 4221 1 4 inch 55 4209 55 4214 55 4210 55 4215 55 4211 55 4216 55 4213 55 4217 55 4222 55 4223 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Appendix C Min Max Flow Rates Minimum flow rates are taken from the smallest inside diameters and maximum flow rates are taken from the largest inside diameters of the syringes supplied by the eleven most widely used syringe manufacturers Nominal Minimum Maximum Flow Rates for Various Syringes Actual Limits will vary depending on manufacturer ml min Min 10 pl 0 0019 1 9013 25 ul 0 0046 4 7752 50 ul 0 0092 9 5511 100 ul 0 0183 19 153 250 pl 0 0454 47 532 500 pl 0 0911 95 492 1000 ul 0 0031 190 95 1 ml 0 0033 205 30 2 ml 0 0119 747 35 2 5 ml 0 0076 476 21 3 ml 0 010 11 231 5 ml 0 0208 21 781 10 ml 0 0301 31 486 20 ml 0 0523 54 804 30 ml 0 0673 70 518 50 ml 0 1019 106 76 100 ml 0 1740 182 40 140 ml 0 2106 220 82 Stainless Steel Syringes Only Harvard s stainless steel syringes can be
18. F gt Request to set the TTL logic level at the specified pin on the external 9 pin D SUB connector Valid pin number for output is 4 If the pin specified is invalid An Out Of Range response will be given Example Set pin 4 high OUT 4 ON Set pin 4 low OUT 4 OFF E Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Pump Chain Commands SEQ lt integer gt lt entry gt Request to set or query programming sequences lt integer gt is sequence number Default is Sequence 1 Command only applicable while pump is stopped Valid sequence numbers n are 1 to 10 n defaults to 1 wherever it is optional See program examples Query entire program SEQ Example response SEQ 1 DISPENSE 75 000 ml mn 43 155 ml 0 00 01 INTERVAL 3 REPEAT INFUSE SEQ 2 PROFILE 100 00 ml mn 150 00 ml REFILL SEQ 3 RESTART Query program sequence n SEQ n Example response to the command SEQ 2 with the previous example s program SEQ 2 PROFILE 100 00 ml mn 150 00 ml REFILL Query program sequence n s mode SEQ n MOD Response will be according to the following table Response Description Response STP stop RST PRO profile GOT INC increment EVN DEC decrement PMP DIS dispense OUT PAS pause Description restart go to event pump TTL out i Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Pump Chain Commands Query data item of program sequ
19. NFUSE OFF PROG1SEQ 9 GOTO 5 PROG1SEQ 6 PROFILE This is an example of the PHD 2000 being operated from a remote location When the pump is powered on the position of the pusher block is unknown and must be homed to aknown position At the syringe full position a limit switch is placed such that it is tripped by the pusher block when the syringe is full The limit switch is connected to pins 4 and 9 programma ble output and the event input on the TTL connector When the pump receives a start sig nal it first refills the syringe and stops when the limit switch is sensed The pump then waits for a start trigger and performs a dispense then refills the syringe and waits again for the next start trigger e next start trigger PROGRAM PRINTOUT PROG1 SEQ 1 EVENT 75 000 ml mn GO TO7 PROG1 SEQ 7 DISPENSE PROG1 SEQ 2 TTL OUT 10 000 ml mn ON 0 0001 ml PROG1 SEQ 3 PAUSE 1 REPEAT 0 00 01 INTERVAL REFILL PROG1 SEQ 4 TTL OUT PROG1 SEQ 8 PUMP OFF 75 000 ml mn PROG1 SEQ 5 PAUSE 10 000 ml 00 00 01 INTERVAL INFUSE PROG1 SEQ 6 PUMP PROG1 SEQ 9 RESTART i Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Pump Chain Commands The Pump Chain RS 232 interface is used to enhance the control applications of the PHD 2000 This interface allows all control information including a program to be entered into the pump from an external source such as a computer In addition this interface allows up to 100 pumps and i
20. PHD 2000 Syringe Pump Series User s Manual PHD 2000 Infuson 70 2000 PHD 2000 Infusion Withdraw 70 2001 PHD 2000 Programmable 70 2002 PHD 2000 VHP 70 2023 ae a Wan HARVARD APPARATUS E Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Table of Contents 1 2 3 4 5 Warranty and Repair Information 4 Manual Description ois csccecscctcecdcestucedeueerececeieniant 4 Warranty cece cectatattedeunbatancecetinnmennebiutiot Hemmmnsdedentned 4 Repair Facilities and Parts ccceceeeeeees 4 Se rial NUMDBIS xiraccsceucioutuidiwndenenierescruteduecemactond 4 COM ONS viceri 4 General Safety Summary ceeeeeettereeeeeees 5 Specifications and Ordering Information 6 7 Theory Of Operation ccccssssseeeeeeeeeeseees 8 Pan aici seen E E A A A 9 Pressure and Speed cceeecsccceeeeeeeseeeeeeeees 9 Built in Syringe Table and Custom Syringes 9 Infusion and Refill Rates s csiasesrderenradscrsadncsnarae 9 Target Vol m E sissen eee ere ee emote ee ee 9 Auto Fill Programmable Mode Only 9 Modes of OG atlon ic cccccccese cennceccaserenestoormensnenecs 9 External Connections sseeessseseeaeees 10 Nonvolatile Memory cccceeeeeeeteeeeeeeeeeeees 10 Stall DSTO CHOM ceiscauiricenentreucdtcddincanerconswanesece loaves 10 Visual Audible Alarm ccccccecceeeeeeeeeeeeees 10 Power Up Options Pu
21. See Figure 4 located directly above the power cord receptacle on the rear panel The two4ine display will illuminate indicating that the power connections are correct The flashing display indicates that power has just been applied Pressing any key will reset the blinking display to constant illumina tion See Section 7 for operating Instructions on next page To change theVoltage setting from 115V to 230V turn off the Main Power switch 0 and remove the power cord Pry open the access door using a small screwdriver Refer to A in Figure 4 Remove the red fuse holder assembly by prying it out of the switch assembly using asmall screw driver Rotate the red fuse holder 180 and install it back into the switch assembly Close the small access door firmly until fully closed 230V should be showing through the small opening in the switch assembly Install the power cord Fuse Replacement Turn off power remove power cord from power module Use straight blade screw driver pry open access door Remove fuse holder remove fuse s from holder Replace fuse s and fuse holder Replace holder with correct voltage marking is showing through access door window For continued fire pro tection replace fuse only with 250V fuse of the specified type and rating 3AG 1 2 AMP SLO BLO manufacturer may impair the protection provided f WARNING Use in manner not specified by the by the equipment 4 Harvard Apparatus PHD 2000 Syringe Pump
22. Spare Parts 52 I Kits and Upgrades ceeeeeeceeeeeeeees 53 J Custom Applications ccceeeeeeeees 54 K Symphony eee cece ee eeeeeeeeceeeeeeeeeneees 54 5 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Warranty and Repair Information CAUTION Verify operating voltage selection before AN plugging in the PHD2000 Pump Refer to Section 6 Initial Setup Manual Description This manual is designed to provide all operational and program information required to operate and maintain all three models of the PHD 2000 series pumps The three models are Infusion Infuse Withdraw and Programmable The functions and features of these models are described in the Technical Specifications section Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to dam age resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A P
23. also allow the selection of special features Refer to Entering Data on page 7 INFUSE RATE Displays sets current infuse rate Scrolls through rate units with each press of the key when setting Displays current programmed rate while running in pro gram mode TARGET VOLUME Displays sets current volume mode target volume PROGRAM Displays current program number program sequence and sequence num ber Held down with a printer attached and the pump stopped prints a program listing Not applicable to the Infusion or Infuse Withdraw models DIAMETER Displays sets current syringe diameter When held down during setting accesses built in syringe table REFILL RATE Displays sets current refill rate Scrolls through rate units with each press of the key when setting Not applicable to the Infusion model RS 232 Displays sets current RS 232 device s attached AUTO FILL TurnsAuto Fill feature setting on off Also displays sets syringe refill volume l N J Toggle Keys Keys in this group when permitted successive states of the keys function are selected when the key is pressed RUN STOP Starts stops interrupts running of pump INFUSE REFILL Changes direction of pusher block travel during operation in Pump and Volume modes Pump must be stopped or in Pump Mode to reverse direction of pump Not applicable to the Infusion model SELECT MODE Toggles in an incremental loop through PUMP VOLUME or PROGRAM run modes wit
24. an increment is one second it will take 59 steps to reach the target rate Sequence 2 starts at time 1 second and ends at time 60 seconds giving it a duration of 59 seconds At one second a step 59 seconds divided by one second per step equals 59 steps The increase per step will by 20 ml mn minus 10 ml mn divided by 59 steps or 0 1695 rounded to four decimal places Continue running at 20 ml mn for 10 seconds with a profile operation Stop the pump i Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Programming Tutorial Multiple Dispensing Example PROGRAM PRINTOUT PROG1 SEQ 1 DISPENSE 35 000 ml mn 15 000 ml 3 REPEAT INFUSE PROG1 SEQ 2 DISPENSE 65 000 ml mn 25 000 ml 2 REPEAT INFUSE PROG1 SEQ 3 DISPENSE 45 000 ml mn RATE miming EXTERMAL TRIGGERS TEE 7 17 000 mi 2 REPEAT Figure 8 Multiple Dispensing INFUSE PROG1 SEQ 4 STOP Here a series of dispenses are programmed Each dispense is started by a trigger such as pressing the RUN STOP key or pressing an attached foot switch Seven dispenses are programmed three of 15 ml at 35 ml mn two of 25 ml at 65 ml mn and two of 17 ml at 45 ml mn The pump s display will show TRIGGER when it is waiting for a run trigger and lt or gt will not be displayed This is a Strategy 1 dispense A time interval of 0 is specified when entering a Strategy 1 dispense Since the total volume to be dispensed is 129 ml and the syrin
25. ard phone jack 55 4202 SS Syringe 200 ml with 1 16 inch Swagelok 55 4208 SS Syringe 200 ml with 1 8 inch SWAGELOK 55 4213 SS Syringe 200 ml with 1 4 inch SWAGELOK 55 4217 SS Syringe 200 ml with Luer Lock For PHD 2000 HVP Remote Syringe Pump For PHD 2000 Push Pull Syringe Pump Spare Parts 0606 086 Magnalube Lead Screw Lubricant 2400 243 Syringe Clamp 5012 005 3 32 Hex Key 5091 030 Syringe Retainer Knob 5100 055 T Knob Withdraw and Ant Siphon Bracket 5153 209 Power Cord 5400 001 PHD2000 Operators Manual 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Appendix I Kits and Upgrades Catalog No Description Kits 70 2012 PHD 2000 6 10 Multirack Upgrade Kit 70 2013 PHD 2000 4 x 140ml Multirack Upgrade Kit 70 2014 PHD 2000 Microliter Rack Upgrade Kit 70 2015 PHD 2000 Anti Siphon Kit for Infusion Pump Upgrades 70 2016 PHD 2000 Infusion to Infuse Withdraw 70 2017 PHD 2000 Infuse Withdraw to Programmable 70 2018 PHD 2000 Infusion to Programmable Factory Installed Only i Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Appendix J Custom Applications The Harvard PHD 2000 Syringe Pumps lend themselves to a multitude of OEM indus trial applications For all types of custom pumping or pilot plant applications the pump s programming capabilities can be enhanced Please contact the Harvard Development Group if we can be of help Appendix K Symphony Sympho
26. atures include two addi tional function keys on the keypad REFILL RATE key and the INFUSE REFILL key and the addition of syringe Retaining Brackets and Adjustable Stops for refill Use of the REFILL RATE key the INFUSE REFILL key Retaining Brackets and Adjustable Stops are all described in detail in section 7 of this manual The PHD 2000 Programmable model is equipped with all the functions of the PHD 2000 Infuse Withdraw model along with additional features to provide Programmable functions The PHD 2000 Programmable models additional features include two addi tional function keys on the keypad the PROGRAM key and the AUTO FILL key Use of the PROGRAM key and the AUTO FILL key are described in detail in section 7 of this manual 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Features Pressure and Speed PHD 2000 can deliver up to 220 82 ml minute with asingle 140 ml syringe Maximum pressure is dependent on syringe size For example a Becton Dickinson Plasticpak 50 60cc syringe run at 5ml minute delivers an average maximum pressure of 1 8kg per cm2 25psi Built In Syringe Table and Custom Syringes If a non standard syringe is to be used enter the inside diameter of the syringe in mil limeters If a standard syringe is to be used use the pump s builtin syringe table Syringes are arranged according to manufacturer and material and then according to size The pump will look up and use the diameter for the
27. ce i Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Appendix F RS 232 Specifications Pump Chain Pump Port 1 Computer control side Pump Port 2 Connection for remainder of pump chain Baud Rate 1200 2400 9600 or 19 200 Word Size 8 Parity none Stop Bits 2 Scale Connect scale to Pump Port 1 Supported scales Mettler Baud Rate 2400 Word Size 7 Parity even Stop Bits 1 Pause 0 Sartorius Baud Rate 2400 Word Size 7 Parity even Stop Bits 1 Code Settings 311 Weigh only models with PLUS performance package 211 External print command with out stability 225 2400 baud 234 Even Parity 432 Beeper off GND GND Transmit Receive Transmit Receive GND GND Port 2 out in Figure 14 RS232 Specifications Port 1 Ohaus Baud Rate 2400 Word Size 8 Parity even Stop Bits 1 Auto print feature off Stable data only off Serial data frame selection Fr 7 Printer Serial printer with print buffer Baud Rate 2400 Word Size 8 Parity none Stop Bits 2 E Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Appendix G Maintenance amp Troubleshooting Maintenance PHD 2000 series pumps require no special maintenance other than keeping them clean by avoiding accidental spills of pumped material The two guide rods and the lead screw should be sparingly lubricated periodically The gu
28. ch it is dropped This out put pin can be attached to the timer input of another pump such as a Harvard Pump 22 or 44 to create a precise mixture during the bolus After the bolus is completed the Pump 22 would be stopped and the PHD 2000 would return to delivering its back ground rate of 300 ml hr waiting for another external event PROGRAM PRINTOUT PROG1 SEQ 1 TTL OUT 5 0000 ml OFF INFUSE PROG1 SEQ 2 EVENT PROG1 SEQ 5 TTL OUT GO TO 4 ON PROG1 SEQ 3 PUMP PROG1 SEQ 6 PROFILE 300 00 ml hr 75 000 ml mn INFUSE 10 0000 ml PROG1 SEQ 4 PROFILE INFUSE 75 000 ml mn PROG1 SEQ 7 RESTART 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Programming Tutorial i Figure 12 Use of TTL Sgnal Before the pumping flow begins the pump sends aTTL signal to another piece of equipment for 5 seconds but first verifies that the TTL line is low by turning it off for 1 second then turns it on The pumping process consists of an alternation of pumping 3 ml s at 53 ml mn and 5 ml s at 75 ml mn It uses the TTL output to signal to another device whose sequence it is executing by lowering theTTL line before sequence 5 and raising it before sequence 8 PROGRAM PRINTOUT PROG1 SEQ 1 TTL OUT 53 000 ml mn OFF 3 0000 ml PROG1 SEQ 2 PAUSE INFUSE 0 00 01 INTERVAL PROG1 SEQ 7 TTL OUT PROG1SEQ 3 TTL OUT ON ON PROG1 SEQ 8 PROFILE PROG1 SEQ 4 PAUSE 75 000 ml mn 0 00 01 INTERVAL 5 0000 ml PROG1 SEQ 5 TTL OUT I
29. display indicates pump chain commu nication has been received 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual User Interface Setting displays are used to facilitate entering control information and datainto the pump Data entry will be discussed in detail later in this section Informational messages occur at various times to indicate such items as a data setting out of range or a detected prob lem such as the pump stalling Pressing any key clears the message from the display The second line of the display is also divided into 3 sections The first is the Running Mode Pump Vol Prog The second section is the AutoFill indication no message if Auto Fill is Off If On AutoFill ON is displayed The third section is a pair of directional arrow heads lt or gt indicating that the pump is running and in what direction AutoFill is not appli cable to the Infusion and Infuse Withdraw Models DESCRIPTION OF KEYS The keypad consists of 19 keys on the Infusion model 21 keys on the Infuse Withdraw model and 23 keys on the Programmable model The keys are used for entering control information and data into the pump These keys are grouped into 3 sections see Figures 1 2 amp 3 Set keys Toggle keys and Data Entry keys Set Keys C SET Allows modification of a data item in this group of keys To modify a data item press the relevant key after pressing the SET key Pressing Set with an appropriate data entry key will
30. e pump If pump is already pumping a Not Applicable response will be given STP Stops pump if it was running If pump was already stopped a Not Applicable response will be given DEL Request for volume delivered in ml Response is of the following format space space f f f f f f CLD Request to zero volume delivered If the pump was interrupted it will can cel the interrupted condition If the pump is running request will not be accepted and a Not Applicable response will be given Otherwise no response is given RAT lt float gt lt units gt Request to set or query infusion rate setting Set infusion rate RAT rate Set infusion rate and units RAT rate units Rate is of format f f f f f f Units are one of Definition UM ul mn UH ul hr MM ml mn MH ml hr If rate is accepted and valid rate will become the new infusion rate If the rate is invalid an Out Of Range response will be given Command will not be accepted if the pump is running in the Program Mode and a Not Applicable response will be given Query infusion rate RAT Response is of the following format space space f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Pump Chain Commands RFR lt float gt lt units gt Request to set or query refill rate setting Set refill rate RFR rate Set ref
31. ence n Command Description SEQ n RAT Query rate Response ff ff ff units Where units is one of the following ml mn ul mn ml hr ul hr SEQ n GOT Query go to sequence number Response lt n gt SEQ n TGT Query target volume Response lt float gt SEQ n INT Query time interval Response lt time gt SEQ n RPT Query repetition count Response lt float gt SEQ n OUT Query output pin level setting Response lt ON OFF gt SEQ n DIR Query pumping direction Possible responses INFUSE REFILL Set mode of program sequence n SEQ n MOD mode Where mode is as follows Mode Description Mode Description STP stop RST restart PRO profile EVN event INC increment GOT go to DEC decrement OUT set output pin DIS dispense PMP pump PAS pause Set data item of program sequence n Set sequence s rate SEQ lt n gt RAT lt float gt lt units gt Rate is of format f f f f f f Units are one of Description UM UH MM MH ul mn ul hr ml mn ml hr 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Pump Chain Commands VER Set sequence s go to sequence number SEC lt n gt GOT lt n gt Set sequence s target volume SEQ lt n gt TGT lt float gt Set sequence s time or target SEQ lt n gt INT lt time gt Set sequence s repetition number SEQ lt n gt RPT lt integer gt Set sequence s pumping direction SEQ lt n gt DIR lt INF REF gt Set sequence
32. er s Manual Pump Chain Commands Each command sent to the pump chain is a string of ASCII characters with leading zero s on numbers and all spaces optional Numbers are a maximum of five digits The following symbols are used in describing the commands Pump Chain Commands Model 22 Protocol Model 44 Protocol on page 37 After each transmission to the Pump terminating with a CR character ASCII 13 the pump enters remote mode and responds with the three character sequence CR LF prompt The prompt character indicates the status of the pump as follows When stopped ASCII 58 decimal gt When running forward ASCII 62 decimal lt When running reverse ASCII 60 decimal When stalled ASCII 42 decimal Note With a pump chain if no address precedes the command trans mitted the pump at address 0 will respond to the command Commands Response CR LF prompt RUN Infuse forward direction REV Start reverse direction Not accessible on Infusion model STP Stop CLV Clear volume accumulator to zero CLT Clear target volume to zero MLM number Set rate units are milliliters per minute ULM number Set rate units are microliters per minute MLH number Set rate units are milliliters per hour ULH number Set rate units are microliters per hour MMD number Set diameter units are mm Rate is set to 0 MLT number Set target infusion volume units are ml Numbers between 0 and 1999 will be accepted by the pump Leadi
33. ess the SET key and then press 1 to select either Model 22 or Model 44 Protocol Refer to Section 11 Pump Chain Commands Press the SET key then use the RS 232 key to scroll through the menu of allowable RS 232 configurations Possible configu rations are Pump Chain Scale Printer Scale amp Printer After entering the RS 232 configuration additional information may be requested Pump Chain Enter the 2 digit address assigned to the pump Note Each pump in the chain needs a unique address After entering the address up to 99 the baud rate will be requested Use the RS 232 key to toggle between the supported baud rates 1200 2400 9600 and 19200 Note Each pump in the chain must have same baud rate See the Section 11 Pump Chain Commands for pump chain control information 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual External Control and Interfaces Scale Use the RS 232 key to toggle between the supported manufacturers Mettler Sartorius and Ohaus When a scale is attached the weight will be read from the scale and used as the delivered volume whenever the pumping direction of the pump is set to infuse When refilling the syringe diameter is used for volume calculations When the scale weight is displayed the units will be grams Printer No additional information requested when entering With a printer capable of serial port communication attached the pump will print the delivered volume whenever
34. ge volume is 50 ml the Auto Fill feature would be very useful with this program s Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Programming Tutorial Periodic Dispense Loop Example RATE minin a TINE mircseconca Figure 9 Periodic Dispense Loop This is an example of a series of periodic dis penses of varying volumes and intervals For this application Strategy 2 dispenses are used Note that between the third and fourth dis penses is a 45 minute interval Each dispense in the first sequence is separated by a pause inter val of 1 30 Since after the third dispense there already will be a 1 30 pause an additional pause of 43 30 is used to extend the pause to the desired 45 00 Sequence 5 is a RESTART command causing the series of dispenses to be continuously repeated until the pump is stopped PROGRAM PRINTOUT PROG1 SEQ 1 DISPENSE 15 000 ml mn 3 5000 ml 0 01 30 INTERVAL 3 REPEAT INFUSE PROG1 SEQ 2 PAUSE 0 43 30 INTERVAL PROG1 SEQ 3 DISPENSE 25 700 ml mn 6 7500 ml 0 05 00 INTERVAL 2 REPEAT INFUSE PROG1 PENSE 20 000 ml mn 4 3000 ml 0 02 30 INTERVAL 4 REPEAT INFUSE PROG1 SEQ 5 RESTART SEQ 4 DIS E Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Programming Tutorial Combination of Infusion and Withdraw Profiles Example Here is an example of a more com plex profile program Each run of the infusion has been determined to pump 43 155 ml T
35. gic level of the programmable output pin 4 Use the PROGRAM key to change the setting Go to Sequence Number Enter the destination sequence to continue operation of the program Valid sequence numbers are 1 to 10 Profile Runs at specified flow rate until target volume is pumped or a time interval has elapsed Travel direction is as specified Data Specified Strategy 1 Flow rate Strategy 2 Flow rate Volume Time Interval 00 00 00 Time Time interval Target volume Pumping direction Pumping direction Incr Increments current rate by specified value and pumps until the target volume is pumped or atime interval has elapsed Units of rate will be that of the current rate of the pump or the infusion rate s units if first sequence Sequence is repeated the specified number of times Travel direction is as specified Data Specified Strategy 1 Delta flow rate Strategy 2 Delta flow rate Volume Time Interval 00 00 00 Time Time interval Volume increment Number of repetitions Number of repetitions Pumping direction Pumping direction 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Program Mode Decr Same as INCR except rate is decremented Dispense Repeatedly dispense specified volume Runs at specified flow rate until a volume is pumped or a time interval has elapsed then pump will stop If no time interval was specified Strategy 1 the display will show TRIGGER and the next dispense will
36. h each press of the key The current mode is displayed on the 2nd line of the display Program mode is only available on the Programmable Model Data Entry Keys 1 2 3 4 5 6 7 8 9 0 Used to enter numeric data values or access special features ENTER Saves and stores displayed data value in memory when setting a data item 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual User Interface ENTERING DATA Set Keys C Keys with rectangular outlines in this group are used to modify or review settings of the pumps control data To review the current setting of a control data item simply hold down the relevant key and the data setting will appear in the top line of the dis play In the case of the PROGRAM key Programmable model only if it is held down the data will be sent to the printer if one is attached To modify a data setting first press then release the green outlined SET key The dis play should then read SET WHAT Press the key in the SET key group whose data is to be modified The display will display the current setting Data is entered into the pump by either entering a numerical value or by scrolling through a menu of choices ways press the green outlined ENTER key to terminate and store each data request by the pump gt If you are to enter a numerical value the far left of the display will show ENTER fol lowed by the units of the number to be entered Using the numerica
37. he first sequence refills the syringe with the volume to be infused then the infusion profile is started after which the syringe is refilled and the infusion is repeated until the pump is stopped Io o 1 x p di a THE jiton Figure 10 Combination Infusion and Withdraw Profiles PROGRAM PRINTOUT PROG1 SEQ 1 PROFILE PROG1 SEQ 5 PROFILE 75 000 ml mn 95 000 ml mn 43 155 ml 0 00 10 INTERVAL REFILL INFUSE PROG1 SEQ 2 PROFILE PROG1 SEQ 6 PROFILE 50 000 ml mn 30 000 ml mn 0 00 04 INTERVAL 0 00 05 INTERVAL INFUSE INFUSE PROG1 SEQ 3 DECR PROG1 SEQ 7 PROFILE 4 0000 DECR 65 000 ml mn 0 00 01 INTERVAL 0 00 05 INTERVAL 12 REPEAT INFUSE INFUSE PROG1 SEQ 8 DECR PROG1 SEQ 4 INCR 5 0000 DECR 5 0000 DECR 0 00 01 INTERVAL 8 0000 INCR 11 REPEAT 0 00 01 INTERVAL INFUSE 8 REPEAT PROG1 SEQ 9 RESTART INFUSE 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Programming Tutorial Use of Events a Output Pin4 0 et aT 4 A Pg Y 75 ae Rate c 5 Ep Time Figure 11 Use of Events This is an example of the PHD 2000 working interactively with other laboratory equip ment The pump will continuously pump at 300 ml hr until an external event a high to low transition at pin 9 possibly produced by another PHD 2000 causes the pump to deliver a 15 ml bolus at 75 ml mn After delivering 5 ml of the bolus output pin 4 is set to a logic high for the duration of the bolus after whi
38. ide rods and the lead screw should be lubricated with Magnalube G R grease Catalog 0606 086 provided with the pump Solvents of any type should never be used to clean the pump A mild detergent solu tion may be used to clean the keypad Troubleshooting Infusion Accuracy To ensure infusion accuracy always use new syringe s and measure syringe bore diameter and enter actual dimensions in millimeters mm Additionally make sure that the guide rods and lead screw are properly lubricated RS 232 Difficulties Verify that the baud rates and data framing parameters on all devices are the same With the pump chain a Communication timeout error on a computer is usually caused by the computer errantly handshaking on the RTS CTS and DSR lines Verify pins 4 5 and 6 on the 25 pin connector are jumped on the computer side of the cable PHD 2000 Display Messages Pump Stalled This indicates that the pusher block travel has been impeded This may be caused by the syringe plunger hitting bottom a kink in the tubing syringe plunger bind ing or any situation requiring more force to the head of the syringe than the pump is capable of delivering To restart the pump remove the cause of stalling and press RUN The pump will continue the interrupted procedure where it stopped Also note a large increase in the pumping rate could also stall the motor Out of Range A value was entered or encountered in a pump program that was beyond the p
39. ill rate and units RFR rate units Rate is of format f f f f f f Units are one of Definition UM ul mn UH ul hr MM ml mn MH ml hr If rate is accepted and valid rate will become the new refill rate If the rate is invalid an Out Of Range response will be given Command will not be accepted if the pump is running in the Program Mode and a Not Applicable response will be given Query refill rate RFR Response is of the following format space space f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr PGR Request for the rate of pumping set during the running of a program Response is of the following format space space f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr 5 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Pump Chain Commands DIA lt float gt Request to set or query syringe diameter setting Set diameter DIA diameter Diameter is of format f f f f f f Units are MM INFUSE and REFILL rates will be set to zero and AUTO FILL will be set to off If diameter is accepted and valid diameter becomes new diame ter Diameter will not be accepted if the pump is running and a Not Applicable response will be given If the diameter is invalid an Out Of Range response will be given Query diameter DIA Response is of the following format space space f f f f f f Units are MM TGT lt float gt
40. ive diameters Infuse Rate The Infuse Rate is the rate of pumping while infusing in the Pump or Volume modes Also the Infuse Rate is used as a starting rate for the program model if one is not spec ified in the program regardless of pumping direction Programmable model only When entering the Infuse Rate the INFUSE RATE key is used to scroll through the allowable units of rate The allowable units are ml mn ml hr pl mn pl hr While run ning in the Pump or Volume modes the Infuse Rate can be changed If the new rate is valid it will take effect when the ENTER key is pressed The minimum and maximum rates permitted vary depending on the diameter of the syringe If an OUT OF RANGE message is displayed when entering a rate try using a different syringe for your appli cation 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Operation Refill Rate The Refill Rate is the rate of pumping while refilling in the Pump or Volume Modes or during Auto Fill If the Refill Rate hasn t been set rate is 0 the Refill Rate will default to the Infuse Rate Refill Rate is not applicable to the Infusion model Auto Fill is applicable to the Programmable model only When entering the Refill Rate the REFILL RATE key is used to scroll through the allow able units of rate The allowable units are ml mn ml hr ul mn pl hr Refill Rate is not applicable to the Infusion model While running in the Pump or Volume modes
41. l keys on the right side of the keypad see Figures 1 2 amp 3 enter the new data value Up to five digit num bers are accepted including up to four decimal places Entering more than five digits will clear the previous five digits entered in the display Press the green outlined ENTER key when the desired data value is displayed If the far left of the display does not show ENTER then a menu of choices is being displayed Pressing the relevant key according to the choices being displayed selects successive menu entries When the desired selection is displayed press the green outlined ENTER key If the data value entered is outside the pump s operating parameters the display will read OUT OF RANGE Pressing any key will restore the display with the original data value Enter another data value within the pump s parameters or just press the green outlined ENTER key to reuse the original data value The data value entered can be reviewed as described above Note Certain data items have multiple settings For these after the ENTER key is pressed the display will prompt you for the additional information Various rules apply to when what and how data can be set at various times See the relevant section for further details SPECIAL FEATURES ACCESS TABLE Pressing the green outlined SET key and then the appropriate numerical key will allow the setting of several special features Pressing the appropri ate numerical key again
42. lowing enough space for the syringe being used to fit between the Pusher Block 2 and the Syringe Holder Block 3 2 Loosen the four screws to free the retaining brackets 4 and 5 on the Pusher Block 2 and Syringe Holder Block 3 This step is not required for the PHD 2000 Infusion model which is not equipped with retaining brackets 4 amp 5 or Adjustable Stops 9 3 Locate Syringe Clamp 6 and determine if clamp is in the correct position to firm ly hold the desired syringe Rule of thumb is that if the syringe is 20ml or larger the tips of the clamp should be pointing down If it is required to change the orienta tion of the clamp unscrew the plastic knob located in the center of the clamp by turning it counter clockwise to the left Remove the knob lift the clamp off the shaft flip the clamp over and place back on the shaft then tighten the knob by turn ing it clockwise to the right Refer to Figure 6 4 Lift up on the spring loaded Syringe Clamp 6 and rotate it 90 to allow clearance for the syringe Place the barrel of the syringe on to the Syringe Holder Block 3 and rotate the clamp over the syringe Ensure that the syringe barrel flange and plunger flange are located within the retaining brackets on both the Pusher Block 2 and the Syringe Holder Block 3 This step is not required for the PHD 2000 Infusion model which is not equipped with retaining brackets or Adjustable Stops 9 Lower the Syringe Clamp 6 down on
43. mp Mode Only 10 Program Storage Programmable Mode Only 10 User INCI AGG iscveccsnscenccenecenscnedewstetecansrenasennnsans 11 Description of Keys ascaceaxcctccscotecentencustentesntaueds 12 Entering Data suctecnseticdadnenounneiehiiaaaamncmnnietsuitines 13 Special Features Access Table 0 0 13 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Table of Contents 6 7 8 9 Initial SetU icisicsiis cutsceaniveiiecs sacsantendeininesssaetoenadane 14 PCPA ON ee setsewiceccruansnaceaerennenananttetrnsirteniie 15 20 Syringe Loading ccceeeeseeceeeeeeeeeeeeeeeeeees 15 Running the PUNO ager tascsntecintect deseo enemas tienes 18 Diameter enca ES 18 Infuse Rate nsseeesnnneeeesesernrnrererrrnnneerrrrenneene 18 PSO Rate sessa 19 Target Vol m Euin 19 PUP aes eee cree os ie eee dese or ener este 19 Selecting the Run Mode ccceeeeeeeeees 20 PUNO ModE dacc scesctenetsenceadatecendcanetsesceeceresetaciee 20 Volume Mode ccceeeeeeeeeneeeeeeeeeeeeneeeeeees 20 Program Mode ssisiisiiiccsinsienvinrsasiadsenaniuns 21 24 Program Description pcctcerscsecersienicesiocinseentededat 21 Entering a Program eee ee nee ere eee eee 21 Sequence Operation ceceeceeeeeeeeeeeeeeeeeees 21 Program PriniOUl sccccacescesieceietccanecccdetdeneioicact 24 Program Run Time Error Messages 24 External Control and Interfaces 25 26 RS
44. n certain cases other RS 232 devices to be con trolled from a single RS 232 communication port on a computer Assign each pump in the pump chain a unique address from 0 to 99 This address is used to identify which pump is to receive a command and which pump is respond ing Configure each pump with its assigned address and the baud rate being used See Section 9 External Control amp Interfaces When the control device communicates with the pump a diamond appears on the far right of the display indicating that it is receiving RS232 commands The diamond remains on the display until the pump is turned off or SET RS 232 is entered on the keyboard indicating a change in the RS 232 configuration A pump will not respond to pump chain communication while it is in a setting mode entered when user presses the SET key The pump can still be controlled from the keyboard while it is in a pump chain Control data that is changed via RS 232 will be stored in the pump s non volatile memory After each command is received and executed the pump terminates its responses with a prompt A prompt is a string of ASCII characters sent by a pump The PHD 2000 is capable of using two sets of pump interface commands This will allow users of Harvard Apparatus Model 22 and Model 44 pumpsto use existing pro grams to control the PHD 2000 pumps The two sets of commands are contained on the following pages 4 Harvard Apparatus PHD 2000 Syringe Pump Series Us
45. n may be requested Rate Enter the rate using the INFUSE RATE key to change units Note If the rate entered is invalid an error message will not be given at the immediate time of entry An OUT OF RANGE error message will be given during the running of the program Delta Rate Enter the rate of Increment or Decrement desired The units of the rate cannot be spec ified Units will be the same as the units of the current pumping rate at the time the sequence is executed Target Volume Enter the required delivered target volume of the sequence For increment and decre ment sequences the target volume is an incremental target An incremental target is added to the delivered volume at the start of the sequence 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Program Mode Time Interval Enter the time duration of the sequence in the form hours minutes seconds If sequence Strategy 1 Target Volume is used enter 0 00 00 for the time target The max imum time interval is 9 99 99 Number of Repetitions Enter the number of times the sequence is to be repeated The repetition number can be from 1 to 99 999 Pumping Direction Each sequence that specifies a pumping operation also specifies a pumping direction Use the INFUSE REFILL key to change the pumping direction lt or gt Direction of travel Infuse Refill is not applicable to the Infusion model Pin Level Select either HI or LOW for the lo
46. n product to ensure proper usage Protective A CAUTION Ground Refer to Manual Terminal 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Specifications Specifications PHD 2000 SYRINGE PUMP SERIES Accuracy 1 Reproducibility 0 1 Syringes Min Max 5 wl 140 ml Flow Rate Minimum 0001 pl hr with 0 5 ul Syringe Maximum 220 82 ml min with 140 ml Syringe Calibration Automatic Display 2 line 40 char fluorescent Non Volatile Memory Stores all settings RS 232 Interface Multiplexed dual bidirectional ports RS 232 Connectors RJ 11 4 cond phone plug TTL Connectors 9 pin D Sub connector Linear Force Max Standard 23 kg 50 lbs High Force 30 kg 66 lbs Drive Motor 1 8 Stepper Motor Motor Drive Control Microprocessor PHD 2000 VHP REMOTE SYRINGE PUMP SERIES 1 0 1 Holds 20 to 200 ml Stainless steel syringes 1 5 ul hr 112 ml min Automatic 2 line 40 char fluorescent Stores all settings Multiplexed dual bidirectional ports RJ 11 4 cond phone plug 9 pin D Sub connector 196 kg 433 lbs N A 1 8 Stepper Motor geared Microprocessor from 1 2 to 1 32 microsteppingfrom 1 2 to 1 32 microstepping Motor Step perone From 800 to 12 800 Rev of Lead Screw Step Rate Minimum 27 3 sec step Maximum 416 7 usec step Pusher Travel Rate Minimum 0 18 m min Maximum 190 676 mm min Power 115 230 VAC 50 60 Hz External voltage selection 65 W 0 5 A fuse Voltage Range 95 13
47. ng zeros and trail ing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number received will be rounded to four significant digits if the leading digit is 1 or three significant digits if the leading digit is 2 to 9 T Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Pump Chain Commands Queries Response CR LF Value CR LF Prompt Queries with Numeric Response DIA Send diameter value units in mm RAT Send rate value in current range units VOL Send current accumulated infused volume units are ml TAR Send target volume units are ml VER Send model and version number Value format nnnn nnn The transmitted value is an 8 character string with leading zeros converted to SP char acters ASCII 32 The fifth character is a decimal point ASCII 46 Query Response CR LF Range CR LF Prompt Queries with String Response RNG Send range message Range is a character string one of ML H ML M UL H or UL M Error Responses CR LF CR LF prompt Unrecognized command CR LF O O R CR LF prompt Out of range 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Pump Chain Commands Pump Chain Commands Model 44 Protocol Symbol f d lt cr gt lt f gt lt float gt lt integer gt lt time gt lt text gt Meaning optional select one either or digits 0 9 or a decimal point digits 0 9 carriage return
48. ns running the pump at the settings that were in process when the power was interrupted Program Storage Programmable model only Programmable model can store up to 4 sets of 9 program sequences for later selection E Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual User Interface R D PHD 22 2000 U S Infusion DOL A R PHD 22 2000 TUS Infuse Withdraw HARVARD pD aajao APPARATUS Programmable Set Infuse Target Program Rate Volume Diameter Refill Rate ae A nair C iat Ue ye C iat J UL N J Figure 3 Programmable Model Kapad The user interface consists of a keypad with a display areaThe display consists of a 2 line 40 character alphanumeric vacuum fluorescent display The display will show one of three types of messages Default display setting display or an informational message The top line of the display is divided into three sections The first consists of the deliv ered volume or time interval remaining in an operation expressed with 5 digits plus a decimal point Next is the units of the previous number expressed as ml for milli liters g for grams if a scale is attached or time for a time interval Third is the pump ing direction either IN FU SE or REFILL or the current operating state of the pump A diamond symbol in the last character of the
49. ny the Windows Pump Manager software allows you to control your PHD 2000 pump from your remote computer With Symphony you can orchestrate all pump functions from a virtual control panel via the serial communications link built into every PHD 2000 pump Catalog Number Product 70 3000 Symphony The Windows Pump Manager
50. ontrol an external valve for refilling Additional TTL pins are available for general use See Section 9 and Appendix E RS 232 Multiple pumps can be daisy chained together and remotely controlled from a com puter or any device communicating via RS 232 A scale can be connected enabling the pump to infuse by weight instead of by volume Section 9 A printer can be con nected to record final volumes or weights whenever the pump stops In addition the program entered for the program mode can be listed on a connected printer Both a scale and a printer may be connected simultaneously See Section 9 Nonvolatile Memory All operational data entered into the pump from the keypad or from a computer will be stored including the program On power up the display will blink until the pump receives its first command and all settings from when it was powered down will be recalled Stall Detection An optical detector is used to verify expected movement of the motor If the motor is prevented from turning due to jamming or excessive back pressure the pump will stop and the display will read PUMP STALLED Visual Audible Alarm After a power interruption the alarm can be selected to be a silent flashing display or an audible signal in addition to flashing display Power Up Options Pump Mode only Enables the user to select between a standby mode waiting for a command to begin running after power is interrupted or a restart mode that begi
51. ot required for the PHD 2000 Infusion model which is not equipped with retaining brackets or Adjustable Stops 9 Loosen the front and rear Refill Adjustable Stops 9 on the left side and slide them both to the right until they contact the Pusher Block 2 Then using the Allen Wrench tighten both Refill Adjustable Stops 9 and return the Allen Wrench to its holder With Multiple Syringe Holder Catalog Numbers 70 2003 70 2006 70 2009 These Versions of the PHD 2000 have a saw tooth syringe holder capable of holding up to ten syringes emptying them simultaneously at identical rates This multiple syringe pump will accommodate up to e Ten syringes of any size up to 10 ml e Six syringes of 20ml 100 ml Catalog Numbers 70 2004 70 2007 70 2010 These Versions of the PHD 2000 have a multiple syringe holder which accommodates e Up to four syringes of 30ml to 140 ml To load syringes rotate the spring loaded retainer arm out of the way and lay syringes in V shaped slots with the syringe flange touching the holder Rotate the retainer arm to hold syringes in place If less than the maximum number of syringes are used try to place them symmetrically about the lead screw to present a balanced load Maximum Force The PHD 2000 will generate 29 Ibs of force With a 50 ml syringe it will deliver 25 psi Note The back pressure for each syringe will be divided by the number of syringes used Thus for four 50 ml syringes the maximum p
52. output low indicates Infuse 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Programming Tutorial In the following examples the diameter is 26 7 mm and the infuse rate is 50 ml mn To run a program after entering it select Program mode using the SELECT MODE key and press the RUN STOP key The examples on the following pages include Multiple Infusions Ramping Up Infusion Rate Multiple Dispensing Periodic Dispense Loop Combination Infuse and Withdraw Profile Use of Events and Use of Signal Multiple Infusion Example The following program will instruct the pump to infuse according to the above graph The program instructs the pump to infuse 10 ml at 75 000 ml mn then infuse another 5 ml at 25 ml mn then stop for a total of three sequences Since this graph is Rate vs Volume Strategy 1 will be used when entering the program SEQUENCE 1 Key Presses Explanation SET PROGRAM ENTER 75 INFUSE RATE ENTER 0 ENTER 10 ENTER INFUSE REFILL ENTER Allows selection of Programming mode Press until PROFILE selected Enters selection Enter rate of 75 000 ml mn Press until units are ml mn Enters rate Enter 0 for time this indicates Strategy 1 10 ml is the first target volume Toggles direction to infuse Enters sequence s pumping direction SEQUENCE 2 Key Presses Explanation PROGRAM ENTER 25 INFUSE RATE ENTER 0 ENTER 5 ENTER INFUSE REFILL ENTER Press until PROFILE
53. racket Syringe Block Syringe Clamps Allen Wrench Adjustable Stops Infuse Adjustable Stops Refill Standard End see manual for loading syringes Push Pull Mechanism Posterior Syringe Clamp 2400 414 2 Thumb Nut 5103 050 Stop Plate 2400 418 T Knob 5100 055 Anti Syphon P P 2400 421 Moveable Syringe Holder and Clamp all syringes mount on this holder 1 to 30 ml only see figure below Pulley Cover 0 T Knob 5100 055 2400 411 Syringe Clamp Syringe Holder moveable 2400 412 Set Screw 2 5115 015 Ass y Pusher 2400 410 Figure 5 Syringe Loading Infuse Withdraw or Programmable model shown 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Operation 7 Locate Allen Wrench 7 in its holder on the back of the Syringe Holder Block and remove Loosen front and rear Infusion Adjustable Stops 8 right side and slide them both to the left until they contact the retaining bracket 4 Using theAllen Wrench tighten both Infusion Adjustable Stops 8 8 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the left until the syringe plunger is located as far out of the syringe as intended to travel Then squeeze the Release Tabs 1 and move the Pusher Block 2 to the right just enough so that the end of the syringe plunger is just short of the maximum trav el allowable and release the Release Tabs 1 This step is n
54. ressure developed in each syringe will be 6 3 psi Syringe Loading Push Pull Mechanism 1 Loading syringes on the standard end see manual 2 Loading syringes on push pull end 1 ml through 30 ml only Remove 2 thumb nuts and syringe clamp Place syringes on moveable syringe holder Adjust syringe retainer using 2 set screws Position moveable holder for correct stroke Clamp on to guide rod with the T knob Lock stop plate in place with thumb nut Place syringe clamp over syringes and lock in place with thumb nut T Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Operation Running the Pump Pressing the RUN STOP key starts the pump The pump will operate according to the relevant data entered as interpreted by the selected run mode Pressing the RUN STOP key while the pump is running stops the pump and the right side of the top line of the display will indicate INTERRUPT plus gt for infusing or lt for refilling Refilling is not applicable to the Infusion model This indicates that the pumping operation has been suspended and can be continued Pressing the RUN STOP key again will contin ue the pumping operation at the point that it was interrupted Changing any of the set tings including the pumping direction and the run mode cancels the interrupted Operation and resets the display The pump can also be started and stopped from a remote source Diameter If the inside diameter of the syringe being u
55. sed is known enter the value in millime ters Otherwise access the built in Syringe Table and select the syringe being used After a new diameter is entered directly or via the builtin Syringe Table the Infuse Rate and Refill Rate are set to 0 and the Auto Fill feature is turned off Refill Rate is not applicable to the Infusion model Auto Fill is applicable to the Programmable model only This is done for reasons of safety The maximum diameter that can be entered is 50 mm To access the builtin SyringeTable after pressing the SET key then the DIAMETER key hold down the DIAMETER key for about one second Once the Syringe Table has been accessed each press of the key will scroll through the manufacturers Find the manu facturer and material if applicable of your syringe Press the ENTER key to enter your selection Now using the DIAMETER key again scroll by pressing the DIAMETER key until the size of your syringe is displayed in cc or uL as indicated on the display Pressing the ENTER key will select the size of the syringe and look up and store the diameter The diameter will be displayed until the ENTER key is released Thereafter pressing the DIAMETER key will display the selected diameter In addition the syringe size selected becomes the default Refill Volume when the Auto Fill feature is turned on Auto Fill is applicable to the Programmable model only See Appendix A for a list ing of the built in Syringe Table and their respect
56. selected Enters selection Enter rate of 25 000 ml mn Press until units are ml mn Enters rate Enter 0 for time this indicates Strategy 1 5 ml is the second target volume Toggles direction to infuse Enter sequence s pumping direction SEQUENCE 3 Key Presses Explanation PROGRAM ENTER Press until STOP selected Enters selection and ends program entry RATE m mimh aa SEQUERCE am 0 ba le WOLUME ral Figure 6 Multiple Infusions PROGRAM PRINTOUT PROG1 SEQ 1 PROFILE 75 000 ml mn 10 000 ml INFUSE PROG1 SEQ 2 PROFILE 25 000 ml mn 5 0000 ml INFUSE PROG1 SEQ 3 STOP 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Programming Tutorial Ramping Up Infusion Rate Example HATE fori pez BEQVENCE i Fl i i T il ThE jippon Figure 7 Ramping Up Infusion Rate PROGRAM PRINTOUT PROG1 SEQ 1 PRO FILE 10 000 ml mn 0 00 01 INTERVAL INFUSE PROG1 SEQ 2 INCR 0 1695 INCR 0 00 01 INTERVAL INFUSE 59 REPEAT PROG1 SEQ 3 PROFILE 20 000 mli mn 0 00 10 INTERVAL INFUSE PROG1 SEQ 4 STOP In this example the pump will ramp up from 10 ml mm to 20 ml mn over 60 seconds then continue to run at 20 ml min for another 10 seconds This is a Strategy 2 Program requiring four sequences T 2 Specify the initial rate as a profile of 10 ml mn for one second Specify the ramp up to 20 ml mn Since the minimum resolution of
57. syringe you select See Appendix A for a listing of the standard syringes Infusion and Refill Rates Specify independent rates for infusing and refilling This allows a slow infusion rate then a fast refill If Refill Rate is not set the software defaults to the set Infuse Rate value Target Volume Specify the volume that is to be infused or refilled The pump will run at the rate spec ified until this volume has been delivered when in the Volume mode Auto Fill Programmable Model Only Auto Fill automatically activates an externally attached solenoid Refer to Appendix H for part number and refills the syringe when it is empty This permits infusions to be virtually independent of syringe capacity Modes of Operation Pump Runs continuously in the infuse or refill directions until stopped Refill not applicable to the Infusion model Volume Runs until a specified volume has been pumped or refilled Refill not applicable to the Infusion model Program Pump operates according to a specified sequence of instructions Programmable model only Note All modes interact with Auto Fill feature but it is only available on the Programmable model 5 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Features External Connections TIL Allows pump operations to be synchronized with external devices or by aperson ata distance from the pump Direction of pump travel can be set viaaTTL pin Also aTTL pin is used to c
58. th atime target unless the volume delivered is zero or the pump is stopped at the start of the sequence enter ing it select Program Mode using the SELECT MODE key and press the RUN STOP key T Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual External Control and Interfaces 1 2 AMP S B CAUTION NOT FOR CLINICAL USE r N anc ON HUMAN PATIENTS HARVARD APPARATUS A HOLSTON MA 01746 cari a RS 232 FREQ AMPS OUT SERIAL NO 5006 313 REV 0 508 893 6999 MADE NUSA o GE o ed A 4 4 OUT IN TTL Figure 7 Rear Panel External Connections External devices that can be attached to the PHD 2000 are categorized into either RS 232 devices or TTL devices Pump Chains Scales and Printers are RS 232 devices all other devices are TTL devices See the Appendix F for specification details on attach ing RS 232 devices and Appendix E for TTL RS 232 Devices There are two telephone jack type connectors on the back of the unit See Figure 7 These are the RS 232 ports Looking at the back of the pump the connector on the right is IN and the left is OUT Attach the RS 232 connectors in the appropriate port according to the following chart Device Port Pump Chain computer side IN Pump Chain pump side OUT Scale IN Printer OUT Configuring the Pump for RS 232 Devices Pr
59. tinues this process until it either has reached a STOP operation the pump is manually or remotely stopped or the last sequence has been completed 4 programs with up to 9 sequences each may be entered A sequence consists of a sequence number indicating the order of the sequence a mode indicating what operation the sequence will be performing and the actual data for the operation such as rates and volumes The necessary data specified for each sequence will depend on the strategy used One of two strategies may be chosen for asequence s target Strategy 1 Target Volume pumps until a target volume is reached while Strategy 2 Time Interval pumps until a target time interval has lapsed When Strategy 1 is used enter a time interval of 0 00 00 then you will be prompted for the target volume See the Programming Tutorial for example programs Entering a Program It is advisable to plan out your program prior to entering the program into the pump Press the SET key then the PROGRAM key to begin entering a program The following is a list of possible data that can be requested when entering a program and instructions on entering the data Sequence Operation Use the PROGRAM key to select the sequence s operation Operations that can be selected are Profile Increment Incr Decrement Decr Dispense Event Go To Pause Pump TTL Out Restart Stop When the required operation is displayed press the ENTER key Additional informatio
60. to the syringe CAUTION Do not let the Syringe Clamp snap down on the syringe Doing so may crack or break a glass syringe 5 Ensure that both retaining brackets 4 and 5 are tight against the syringe barrel flange and plunger flange then tighten the screws on the retaining brackets on the Pusher Block 2 and the Syringe Holder Block 3 This step is not required for the PHD 2000 Infusion model which is not equipped with retaining brackets or Adjustable Stops 6 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the right until the syringe plunger is located as far into the syringe as it will travel Then squeeze the Release Tabs 1 and move the Pusher Block 2 to the left just enough so that the end of the syringe plunger is not touching the inside end of the syringe barrel and release the Release Tabs 1 This step is not required for the PHD 2000 Infusion model which is not equipped with retaining brackets or Adjustable Stops 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Operation vo RELEASE disengage ENGAGED 1 2 3 e a 4 5 Release Tabs Pusher Blocks Syringe Holder Block Retaining Bracket 9 Pusher Block Retaining B
61. ume in the syringe reaches the set refill volume Auto Fill will stop and the previous operation of the pump will resume Auto Fill continues to monitor the vol ume of the syringe TTL direction output is toggled ON during refill Refill Rate defaults to Infuse Rate if not set Note Auto Fill will only activate while infusing i e if the pump direction is set to Refill the pump will not stop when the syringe is full Also if the syringe plunger is manually moved the pump will lose track of the true syringe volume J Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Operation Selecting the Run Mode After entering any necessary operating data into the pump select the pumping mode that will be used when the pump is operated Pressing the SELECT MODE key advances the second line of the display to indicate the next run mode Advance the run mode to the desired mode either Pump Mode Volume Mode or Program Mode until it is displayed Pump Mode The pump will conti the Infuse and Refil be changed by pressi Program Mode nuously pump is applicable to the Programmable model only infusing or refilling until stopped While running Rates can be changed The new rate for the relevant pumping direction takes effect when the ENTER key is pressed Also the pumping direction can ng the INFUSE REFILL key Refill Refill Rate and Infuse Refill are not applicable to the Infusion mode Volume Mode The p
62. ump s limits Also verify that the Infuse Rate is not 0 Note that rates must be less than 42949 Express larger rates using different units EEPROM Data Lost The values stored in the non volatile memory have been lost This should never occur under normal operation Replace NOVRAM The non volatile memory has failed This should never occur under normal opera tion 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Appendix H Accessories amp Spare Parts Auto Fill Accessory To operate the Auto Fill feature either the low pressure or high pressure valve assem bly is required Each assembly contains the solenoid valve connector cable power cable and tubing and fittings to connect to the syringe Operates at 115 230 VAC 50 60 Hz via a selector switch Catalog No Description Accessories 55 7760 Daisy Chain Cable 70 2022 Daisy Chain Connector 9 PIN 55 4145 Daisy Chain Connector 25 PIN 55 4144 Foot Switch 55 1146 Auto Fill Valve Box Regular Pressure to 25 psi 55 1145 Auto Fill Valve Box High Pressure 200 psi 59 7357 Manual Liquid Switch 59 7358 Automatic Liquid Switch 115 VAC 60 Hz 59 7359 Automatic Liquid Switch 230 VAC 50 Hz 59 7356 Replacement Tubing Connection Kit 59 7355 Replacement Reducing Adapters 61 0270 Continuous Flow Tube Segment 72 0199 Remote Extension Cable 1 5 m 5 ft 72 1405 Remote Ext Cable 9 1 m 30 ft 70 2022 PHD 2000 RS 232 Adapter 9 pin to stand
63. ump will run refilled The TARGET Used in conjunction infusing or refill ing until a specified target volume is pumped or VOLUME key is used to enter the Volume Mode target volume with Auto Fill the target volume can be greater than the volume of the syringe Whi e running the Target Volume Infuse and Refill Rates can be changed The new rate for the relevant pumping direction takes effect when the ENTER key is pressed Target Volume can be used to enter aTarget weight in grams if a scale is attached via RS 232 Refill is not applicable to the Infusion model Auto Fill is applicable to the Programmable model only E Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Program Mode only applicable to programmable model In the Program Mode the pump can make complex dispenses including changes in rate and target volume These complex dispenses are easily programmed from the key pad and are detailed in the Program Mode and the Programming Tutorial sections Program Description A program is made up of a set of sequences Each sequence being a set of operating instructions for the pump to follow When the pump is started in the PROGRAM run mode the pump will start at sequence 1 and execute the operating instructions in that sequence When the pump has completed the instructions for a sequence it will go to the next or specified sequence and execute the instructions in that sequence The pump con
64. w PHD 2000 Programmable Infuse Withdraw Regular 70 2020 70 2019 Harvard PHD 2000 HVP High Volume and Pressure Standard over 400 Ibs Force Pump Versions PHD 2000 HVP High Volume and Pressure 70 2023 Regular High Power 58 Ibs Force Regular Remote 71 2000 71 2100 71 2001 71 2101 71 2002 71 2102 71 2003 71 2103 71 2004 71 2104 71 2005 71 2105 71 2006 71 2106 71 2007 71 2107 71 2008 71 2108 71 2009 71 2109 71 2010 71 2110 71 2011 71 2111 4 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Theory of Operation All three models in the PHD 2000 series employ a microcontroller which controls a small step angle stepping motor that drives alead screw and Pusher Block Micro step ping techniques are employed to further reduce the step angle eliminating flow pul sation A keypad is used for entry of operating data to the pump Data can also be entered via an RS 232 connector located on the rear panel The microcontroller using the internal Syringe Look Up Table calculates the cross sectional area of the syringe selected and calibrates the flow rate and volume accumulation The numerous features of the PHD 2000 series result from the use of microprocessor technology The PHD 2000 Infuse Withdraw model is equipped with all the functions of the PHD 2000 Infusion model along with additional features to provide the Withdraw Refill function The PHD 2000 Infuse Withdraw model s additional fe
65. will scroll between the options provided Refer to the table below When the desired option is displayed press the green out lined ENTER key RS232 Protocol SET 1 Alarm Mode SET 2 Power Up Mode SET 3 Harvard Apparatus PHD 2000 Syringe Pump Series User s Manual Initial Setup Voltage Selector Serial Number Label CAUTION NOT FOR CLINICAL USE ON HUMAN PATIENTS 000 lll 000000 HARVARD APPARATUS RS 232 1 0 IN HOLLISTON MA 01746 OUT CAT VOLTS oGssso 0000 FRE REQ AMPS SERIAL NO 5006 313 REV 0 508 893 8999 MADE IN USA T Power Cord Socket Main Power Switch a Figure 4 Rear Panel Voltage Selector and Serial Number Label Read the entire manual to become familiar with all features and functions of the PHD 2000 If the operating voltage is other than 115VAC 60Hz locate the voltage selector on the rear panel See Figure 4 of the pump and set it to the voltage being used Also if other than 115 V 60 Hz is being used the plug must be cut off and an appropriate plug installed observing the polarity of the international line cord used Brown live Blue neutral Green ground Take this opportunity to fill out and mail the Warrany Card The Catalog and Serial numbers are located on the Serial Number label on the rear panel Refer to Figure 4 Turn on main power switch

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