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Dionex RFIC-ER Startup Kit Installation Instructions

1. Connect the pump tubing to the inlet of the new ER1 column Connect a short piece of tubing to the outlet of the new ERT column and direct this tubing to a waste container This is a temporary connection Set the pump flow rate to 2 0 mL min and begin pumping the operating eluent through the ER1 column for 20 minutes Make sure the suppressor does not activate during this time If the pump stops running and the Audit Trail displays the Pump stopped because the total gas volume has exceeded the limit message follow these steps a Under ERC 10 Controller on the Dionex RFIC ER Wellness panel click Reset Gas Volume This resets the Gas Volume to 0 00 mL b Restart the pump and suppressor and monitor the Gas Volume reading If the value begins to climb toward 0 60 mL click Reboot ERC 10 under ERC 10 Controller This disables the gas monitor for three hours Doc 065208 04 10 12 57 Dionex RFIC ER Kit Installation Instructions 12 3 c If after three hours the Gas Volume reading is still increasing shut down the system and contact Thermo Fisher Scientific for troubleshooting assistance Do not operate the system with the gas monitor deactivated for an extended period of time 7 After rinsing the ER1 column for 20 minutes turn off the pump 8 Disconnect the temporary waste tubing from the ER1 column outlet reconnect the tubing labeled TO INJ VALVE IN P to the outlet of the ERT column 9 Mount the
2. si Remove the cap from the Dionex RFIC ER eluent bottle and dispose of the expired eluent IMPORTANT Always neutralize methanesulfonic acid eluents before disposal Prepare 4 liters of the eluent required for the application Always use ASTM Type I 18 megohm cm filtered deionized water when preparing eluent Add eluent up to the maximum fill mark on the bottle see Figure 3 or Figure 20 IMPORTANT GE EN Dionex RFIC ER system operation do not reuse expired Under Eluent on the Dionex RFIC ER Wellness panel click the Reset EC button This resets the Operation Time to zero and resets the Remaining Time to 672 hours Reconnect the pump inlet line to the Eluent Bottle Out tubing Connect a short piece of tubing to the ERC 10 outlet and direct this tubing to a waste container This is a temporary connection Prime the pump If you need instructions refer to the operator s manual for the ICS 1000 1500 2000 or Dionex ICS 3000 After priming the pump check that the eluent level is above the minimum fill mark on the eluent bottle see Figure 3 or Figure 20 If necessary add more eluent to the bottle If the eluent level falls below the minimum fill mark the outlet tubing IMPORTANT may not reach the fluid level and the pump may lose prime 10 Select the standard flow rate and current settings for the application Operate the system for 30 minutes to flush the expired eluent from the system If the pump sto
3. 000005 24 7 1 Installing the ERT Column Bracket Clip 24 7 2 Installing the Eluent Bol 26 Doc 065208 04 10 12 i Dionex RFIC ER Kit Installation Instructions 10 11 12 13 7 3 Plumbing the ERT Column for Conditioning 27 74 Conditioning the ERT Column 28 7 5 Installing the ER2 Column 30 7 6 Testing forLeaks 3 Gert Steet una ra n beige erte AER Re 37 Chromel on Sepp 09 EIERE dae ee e EIS ei 39 8 1 Dionex RFIC ER System Requirements 39 8 2 Updating Chromeleon 39 8 3 Updating the Moduleware 40 8 4 Configuring the ERC 10 Properties 43 8 5 Configuring the Dioenx ICS 1000 1500 2000 Properties 46 8 6 Configuring the Dionex ICS 3000 DC Properties 47 Confirming the Dionex RFIC ER Wellness Settings 50 Conditioning the Suppressor 51 Dionex RFIC ER System Operating Guidelines 52 Dionex RFIC ER System Maintenance 53 12 1 Replacing the Eluent 53 12 2 Replacing the ERI Column 56 12 3 Replacing the ER2 Column 58 Troubleshooting 63 13 1 Pump Stops Unexpectedly 0 0 0 0 000000 63 13 2 Unstable Retention Times Dioenx ICS 1000 1500 2000 Doc 065208 04 10 12 Dionex RFIC ER Startup Kit Installation Instructions 1 Introduction Thermo Scientific Dionex Reagent Free Jon Chromatography with Eluent Regeneration Dionex RFIC ER is a lo
4. 10 Connect waste tubing to the ERC 10 outlet see Figure 31 Direct the waste line to a waste container Figure 31 Connecting the ERC 10 Waste Line Do not connect the ERC 10 USB cable to the ICS 3000 DC until instructed to do so 36 Doc 065208 04 10 12 Initial Setup for ICS 3000 Systems 7 6 Testing for Leaks 1 Turn on the pump and suppressor at the appropriate flow rate and current see the following table Separator Column Recommended Maximum Suppressor Flow Rate Current AS4A SC Anion 2 0 mL min 27 mA Separator AS9 HC Anion 1 0 mL min 45 mA Separator AS12A Anion 1 5 mL min 25 mA Separator AS14 Anion Separator 1 2 mL min 25 mA AS14A Anion 1 0 mL min 43 mA Separator AS22 Anion Separator 1 2 mL min 31 mA AS23 Anion Separator 1 0 mL min 25 mA CS12A Cation 1 0 mL min 59 mA Separator CS16 Cation Separator 1 0 mL min 88 mA 2 Begin operating the system for 3 hours i e with the system not operating in the recycled mode of operation During this time periodically check all tubing connections for leaks Tighten any connections that leak After 1 5 hours check the eluent bottle If the eluent level is below the minimum fill mark see Figure 20 turn off the system refill the eluent bottle and restart the system If the eluent level falls below the minimum fill mark the outlet tubing IMPORTANT i S may not reach the fluid level and the pump may lose prime 3 After 3 hours of
5. Backing Plate Screw di Figure 18 ER1 Column Bracket Clip 24 Doc 065208 04 10 12 Initial Setup for ICS 3000 Systems 4 Install the ERT column bracket clip in place of the static mixer clip see Figure 19 When correctly installed the clip is angled about 30 degrees from vertical Figure 19 Installing the ER1 Column Bracket Clip Doc 065208 04 10 12 25 Dionex RFIC ER Kit Installation Instructions 7 2 CAUTION MISE EN GARDE Installing the Eluent Bottle 1 Prepare 4 liters of the eluent required for the application Always use ASTM Type I 18 megohm cm filtered deionized water when preparing eluent 2 Note the minimum and maximum fill marks on the eluent bottle see Figure 20 Add fresh eluent up to the maximum fill mark Maximum Fill Mark 4 L Minimum Fill Mark e About 3 7 L Figure 20 Eluent Bottle Fill Marks If the eluent level falls below the minimum fill mark the outlet tubing may not reach the fluid level and the pump may lose prime 3 Screw the cap onto the eluent bottle Gas Vents Check that the two gas vents are seated in the cap and are free of obstructions see Figure 21 Figure 21 Gas Vents in Eluent Bottle Cap Do not block the gas vents in the eluent bottle cap If the ERC 10 malfunctions the gas vents must be able to discharge any hydrogen and oxygen gases produced by the electrolytic suppressor to
6. Before starting operation review the operating guidelines in Section 11 Dionex RFIC ER System Operating Guidelines For complete operating instructions refer to the operator s manual for the ICS 1000 1500 2000 or Dionex ICS 3000 The guidelines here are intended to supplement not to replace the information in the instrument manuals Dionex RFIC ER is compatible with carbonate bicarbonate eluents for anion separations and methanesulfonic acid eluents for cation separations only Dionex RFIC ER can only regenerate the same eluent that was started with therefore Dionex RFIC ER is compatible with isocratic separations only and cannot be used with detection systems that alter the eluent composition such as post reagent delivery and the CRD 300 Dionex RFIC ER is not compatible with any eluents containing organic solvents The eluents in a Dionex RFIC ER system must be 100 aqueous The capacities of the various trap columns in the Dionex RFIC ER system are limited therefore Dionex RFIC ER is compatible only with low contaminant water samples such as drinking water surface water and ground water During an injection on a Dionex RFIC ER system the equivalent volume of eluent is exchanged with the sample injected Because sample ions are trapped by the columns the eluent is diluted The Dionex RFIC ER system is designed to handle a pre defined volume of injected samples before the eluent needs replacement Injections of samples
7. ERT column in the ER1 column bracket clip 10 Under ER1 Column on the Dionex RFIC ER Wellness panel click Reset ER1 11 Restart the pump and suppressor The system is now ready for routine operation Replacing the ER2 Column When to replace the ER2 column The Dionex RFIC ER Wellness feature in Chromeleon monitors the use of the ER2 column An Audit Trail message will inform you when it is time to replace the column Thermo Fisher Scientific recommends replacing the ER2 column when it expires However it is possible to regenerate the column For instructions on replacing the ER2 column see page 60 For instructions on regenerating the ER2 column see page 61 Never operate an ER2 column beyond its expiration this may result in a buildup of sample co ions in the eluent leading to increased background conductivity and reduced peak areas 58 Doc 065208 04 10 12 Dionex RFIC ER System Maintenance You can check the life ER column expectancy of the ER2 column l 5 Remaining Inj Volume at any time by reviewing the Volume rested status information on the Remaining Inj volume 130000 Dionex RFIC ER Wellness panel For details about the Injections Made 0 Wellness panel parameters see the So GE Remaining Injections 0 Reset ER Volume Injected The total sample volume injected since the ER2 column was installed When the Volume Injected is 120 000 uL the ER2 column has expired Remaining Inj The
8. For more information about the trap columns refer to the column manual provided on the Thermo Scientific Reference Library DVD P N 055405 Figure 1 illustrates the liquid flow path through a Dionex RFIC ER system Injection Valve P veer ER2 Eluent ER Bottle Controller 4 57 mm 0 062 in OD Tubing 3 18 mm 0 125 in OD Tubing Figure 1 Dionex RFIC ER System Fluid Schematic The key to Dionex RFIC ER is operating the suppressor in the recycled eluent mode During the suppression operation the suppressor exchanges the counterion of the eluent and analyte for hydronium in anion systems or hydroxide in cation systems during the regeneration operation the counterion is returned to the eluent and the excess hydronium or hydroxide is neutralized Thus the ion balance is maintained throughout the entire suppression regeneration process e The ER1 is a Thermo Scientific Dionex IonPac Eluent Purification Column anion column AEP ER1 cation column CEP ER1 The ER1 is a mixed bed column that includes matrix trap material The ER1 traps any contaminant species that may be liberated from the separator column or suppressor or introduced in the sample thus ensuring the purity of the regenerated eluent The ERT has a secondary purpose of trapping the matrix counterions introduced by the sample Doc 065208 04 10 12 Functional Description e The ER2 is a Thermo Scientific Dionex
9. N 067789 e Dionex RFIC ER Cation Consumables Kit P N 067792 Each Installation Kit contains all non consumable items required to upgrade an IC system to a Dionex RFIC ER system including the ERC 10 ER1 and ER2 mounting clips eluent bottle assembly and associated tubing Each Consumables Kit contains five ERT columns and one ER2 column Substitution of non Dionex Thermo Scientific parts may impair IMPORTANT k system performance and void the product warranty For details see the warranty statement in the Dionex Terms and Conditions 8 Doc 065208 04 10 12 Procedure Overview 5 Procedure Overview These are the main steps in the Dionex RFIC ER upgrade procedure Install the ER1 eluent purification column bracket clip Install the eluent bottle Plumb the ER1 column for conditioning Condition the ER1 column Complete the ER1 column tubing connections Install the ER2 analyte trap column Install the ERC 10 Test the system for leaks Complete the tubing connections Update the Thermo Scientific Dionex Chromeleon Chromatography Management System software update the ICS 1000 1500 2000 or Dionex ICS 3000 DC Moduleware Configure device properties in the Chromeleon Server Configuration program For a new or regenerated Dionex SRS 300 only Condition the SRS 300 for 16 hours To begin the upgrade procedure for a ICS 1000 1500 2000 system go to Section 6 To begin the upgrade procedure for a Dionex ICS 3000 s
10. cc syringe P N 079803 see Figure 17 Pull the remaining fluid approximately 16 cc from the ERC 10 until only air is exiting the controller Figure 17 Drawing Fluid from the ERC 10 7 Disconnect the union luer adapter and syringe from the ERC 10 inlet tubing 8 Connect the ERC 10 inlet tubing to the suppressor Regen Out port Connect the ERC 10 outlet to the Eluent Bottle In tubing 9 Add eluent up to the maximum fill mark on the eluent bottle see Figure 3 Go on to Section 8 to update Chromeleon and the ICS 1000 1500 2000 Moduleware Doc 065208 04 10 12 23 Dionex RFIC ER Kit Installation Instructions 7 Initial Setup for ICS 3000 Systems In order to ensure that the eluent returned to the eluent bottle is identical in composition to the starting eluent several components in the Dionex RFIC ER system must be equilibrated before initial operation This section describes the temporary connections required for equilibration 7 1 Installing the ER1 Column Bracket Clip 1 Verify that the system is set up as directed in the ICS 3000 operator s manual The manual is included on the Thermo Scientific Reference Library DVD P N 055405 2 Remove the GM 3 or GM 4 static mixer clip from the Dionex ICS 3000 DP SP component mounting panel 3 The ERI column bracket clip P N 066039 consists of a clip a backing plate that orients the clip to the correct angle and a screw see Figure 18 Clip
11. running program with the device Do you want to continue Click Yes 42 Doc 065208 04 10 12 Chromeleon Setup The Moduleware download dialog box appears Please select a firmware file Look in Bin ec Be 00k O Troubleshooting Guides O waveform E ics 1000_2000_2 1 7 fmw 3 1C5 1500_2 0 3 fmw Filename SR Files of type Firmware Files v Cancel 8 Select the new Moduleware file and click Open to begin the download 9 When Chromeleon has finished installing the new Moduleware click OK to close the Properties dialog box 8 4 Configuring the ERC 10 Properties 1 Connect the ERC 10 USB cable to any available USB port on the rear panel of the ICS 1000 1500 2000 or the Dionex ICS 3000 DC 2 Microsoft Windows will immediately detect the ERC 10 and launch the Found New Hardware Wizard Follow the instructions in the wizard to install the ERC 10 driver During installation select the following options e If asked whether Windows can connect to Windows update to search for software select No not this time e Accept the default option Install the software automatically and click Next gt It is not necessary to insert a hardware installation CD ROM or floppy disk Doc 065208 04 10 12 43 Dionex RFIC ER Kit Installation Instructions 3 When the ERC 10 driver has been installed click Finish 4 Inthe Server Configuration program right click the timebase that con
12. total operation time shut down the system 4 Disconnect the waste line from the ERC 10 outlet Doc 065208 04 10 12 37 Dionex RFIC ER Kit Installation Instructions 5 Disconnect the green ERC 10 inlet tubing from the suppressor Regen Out port and allow the fluid to drain into a waste container 6 Connect the ERC 10 inlet tubing to a 1 4 28 union P N 039056 luer adapter P N 024305 and 10 cc syringe P N 079803 see Figure 32 Pull the remaining fluid approximately 16 cc from the ERC 10 until only air is exiting the controller Figure 32 Drawing Fluid from the ERC 10 7 Disconnect the union luer adapter and syringe from the ERC 10 inlet tubing 8 Connect the ERC 10 inlet tubing to the suppressor Regen Out port Connect the ERC 10 outlet to the Eluent Bottle In tubing 9 Add eluent up to the maximum fill mark on the eluent bottle see Figure 20 Go on to Section 8 to update Chromeleon and the Dionex ICS 3000 DC Moduleware 38 Doc 065208 04 10 12 Chromeleon Setup 8 Chromeleon Setup 8 1 8 2 Dionex RFIC ER System Requirements The Dionex RFIC ER system consumables have limited life spans and require periodic replacement or regeneration The Chromeleon Dionex RFIC ER Wellness feature tracks the usage of the consumables and notifies you when they approach the end of their useful life spans When Chromeleon is disconnected from the system the ICS 1000 1500 2000 or Dionex ICS
13. volume of sample that can be injected before the Volume sample volume limit 120 000 uL is reached When the Remaining Inj Volume is 0 uL the ER2 column has expired Resetting the ER2 counter resets the Remaining Inj Volume to zero also When you start the next injection the Remaining Inj Volume will be updated to the correct value Important To ensure the accuracy of the Remaining Inj Volume be sure to enter the correct injection volume in the Inj Vol column of the sequence table Injections Made The total number of sample injections since the ER2 column was installed Remaining The number of sample injections that can be performed Injections before the sample volume limit 120 000 uL is reached The Remaining Injections value is based on the volume of the last injected sample if no sample has been injected yet the reading is zero Reset ER2 Clicking Reset ER2 resets the ER2 column counters resetting Volume Injected and Injections Made to zero Click Reset ER2 only after replacing or regenerating the ER2 column Doc 065208 04 10 12 59 Dionex RFIC ER Kit Installation Instructions How to replace the ER2 column 1 2 Check that the pump and suppressor are turned off Remove the old ER2 column from the ER2 column bracket clip on the suppressor Disconnect the cell outlet tubing from the inlet of the old ER2 column disconnect the suppressor Regen In tubing from the outlet of the old ER2 column Disc
14. 3000 DC Moduleware monitors the consumables usage and stores the tracking information in its permanent memory In order to operate your Dionex RFIC ER system you need e Chromeleon release 6 80 SP3a or later or 6 70 SP6a or later e The appropriate Moduleware version Device Moduleware Version Required Dionex ICS 1000 Dionex ICS 2000 Version 3 0 0 or later Dionex ICS 1500 Version 3 0 0 or later Dionex ICS 3000 DC Version 2 0 0 or later e An ERC 10 If the system is a ICS 1000 1500 2000 Chromeleon automatically activates the Dionex RFIC ER features in the Moduleware when it detects the presence of an ERC 10 If the system is a Dionex ICS 3000 the user must manually activate the Dionex RFIC ER features Updating Chromeleon Check the Chromeleon release installed on the PC If necessary see Section 8 1 run Chromeleon Setup to install the software update Doc 065208 04 10 12 39 Dionex RFIC ER Kit Installation Instructions 8 3 Updating the Moduleware 1 Stop all batches programs and data acquisition events on the Chromeleon Server that hosts the system to be upgraded 2 If itis not already running start the Chromeleon Server Monitor program Select Start gt All Programs or Programs depending on the operating system gt Chromeleon gt Server Monitor 3 The Server Monitor status message should read Chromeleon Server is running idle Chromeleon Server Monitor Status Si Chromeleon Ser
15. 61 Dionex RFIC ER Kit Installation Instructions If the ER2 column is a cation column follow these steps a d Prepare fresh 0 4 M methanesulfonic acid solution Use either reagent grade methanesulfonic acid or undiluted Thermo Scientific CS12A Eluent Concentrate 0 4 Molar P N 057562 Pump 200 mL of 0 4 M methanesulfonic acid solution through the ER2 column Use the Thermo Scientific Trap Column Suppressor Cleanup Kit P N 059659 or an accessory pump at 5 0 mL min Direct the column effluent to a waste container Pump 40 mL of deionized water through the ER2 column Use one of the following the Trap Column Suppressor Cleanup Kit P N 059659 an accessory pump at 5 0 mL min or a syringe Direct the column effluent to a waste container Go on to Step 6 Reconnect the cell outlet tubing to the inlet of the ER2 column reconnect the suppressor Regen In tubing to the outlet of the ER2 column Mount the ER2 column on the ER2 column bracket clip on the suppressor Under ER2 Column on the Dionex RFIC ER Wellness panel click Reset ER2 62 Doc 065208 04 10 12 Troubleshooting 13 Troubleshooting 13 1 Pump Stops Unexpectedly The ERC 10 electronics monitor the volume of hydrogen gas in the eluent stream If the safety limit is exceeded the ERC 10 shuts down the pump and the Audit Trail displays the following message Pump stopped because the total gas volume has exceeded the limit To return
16. Bottle Fill Marks If the eluent level falls below the minimum fill mark the outlet tubing IMPORTANT i may not reach the fluid level and the pump may lose prime 3 Screw the cap onto the eluent bottle Check that the two gas vents are seated in the cap and are free of obstructions see Figure 4 T i Gas Vents Figure 4 Gas Vents in Eluent Bottle Cap Do not block the gas vents in the eluent bottle cap If the ERC 10 malfunctions the gas vents must be able to discharge any hydrogen and oxygen gases produced by the electrolytic suppressor to the CAUTION atmosphere N obturez pas les vents du bouchon de la bouteille d luant En cas de d faillance de ERC 10 les vents doivent pouvoir vacuer vers atmosph re l hydrog ne et oxyg ne produits par le suppresseur MISEENGARDE lectrolytique Doc 065208 04 10 12 Dionex RFIC ER Kit Installation Instructions Blockieren Sie keinesfalls die ffnungen im Deckel der Eluentenflasche Wenn der ERC 10 nicht korrekt arbeitet m ssen die vom elekirolytischen Supressor erzeugten Wasserstoff und VORSICHT Sauerstoffgase Uber diese ffnungen in die Atmosph re gelangen k nnen 4 Connect the Eluent Bottle Out tubing to the pump inlet line 5 Prime the pump 6 Make sure the suppressor has been hydrated as instructed in the SRS 300 QuickStart Suppressor manuals are provided on the Thermo Scientific Reference Library DVD P N 055405 6 3 Plum
17. Controller ERC 2101 Dionex ERC 10 Control e VW Share eluent bottles T Keep pumping even if communcation fails IV Share waste bottle IV Pressure Satz 6 Under Link to ERC Controller select the ERC 10 installed in the system 7 Click OK 8 Exit the Server Configuration program When asked whether to save the configuration changes click Save NOTE Go to Section 9 to check the Dionex RFIC ER Wellness panel settings Doc 065208 04 10 12 49 Dionex RFIC ER Kit Installation Instructions 9 Confirming the Dionex RFIC ER Wellness Settings 1 Launch the Chromeleon client 2 Click the Dionex RFIC ER Wellness tab on the panel tabset in Chromeleon Check the following Wellness panel settings a Verify that the Chemistry Type matches the column chemistry you plan to run b Under ER1 Column ER2 Column and Eluent verify that the counters are set to zero If a counter is set to a value other than zero click the corresponding Reset button c Under ERC 10 Controller verify that the Connected check box is selected and that the Mode is either Operation or Boot System_1 ICS 1500 System with Column Heater System_1 CMADLER1 RFIC ER Wellness Sequence Control Status Chemistry Type Anion_Other gt ERT column Eluent Operation Time Remaining Time Capacity Consumed 0 0 Remaining Time Remaining Capacity Remaining 100 0 Volume injected 0 Remaining Inj Volume ResetER1 Remaining Inj Volum
18. Dionex RFIC ER Startup Kit Installation Instructions Document No 065208 Revision 04 October 2012 2012 by Thermo Fisher Scientific Inc All rights reserved Chromeleon IonPac and SRS are registered trademarks of Thermo Fisher Scientific Inc in the United States Microsoft and Windows are registered trademarks of Microsoft Corporation in the United States and other countries Velcro is a registered trademark of Velcro Industries B V in the United States and possibly other countries PEEK is a trademark of Victrex PLC All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this docume
19. IC ER Kit Installation Instructions 2 Safety Information The ER Controller ERC 10 is an integral element of the Dionex RFIC ER system The ERC 10 electronics monitor the volume of electrolysis gases such as hydrogen and oxygen in the eluent stream If the predefined safety limit is exceeded the ERC 10 shuts down the pump to prevent the buildup of gases in the eluent bottle The ERC 10 is designed for use with IC systems and should not be used for any other purpose Operation of the ERC 10 in a manner not specified by Thermo Fisher Scientific may result in personal injury If you have a question regarding appropriate usage contact Technical Support for Dionex products before proceeding In the U S and Canada call 1 800 346 6390 Outside the U S and Canada call the nearest Thermo Fisher Scientific office 2 1 Safety Messages This manual contains warnings and precautionary statements that when followed properly can prevent personal injury and or damage to the ERC 10 Safety messages appear in bold type and are accompanied by icons as shown below VAN Indicates an imminently hazardous situation which if not avoided will result in death or serious injury DANGER VAN Indicates a potentially hazardous situation which if not avoided may result in death or serious injury WARNING A N Indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury CAUTION Indica
20. IC ER system is a Dionex ICS 3000 go to Section 8 6 NOTE Iftwo ERC 10 controllers are to be placed in the same timebase rename the second controller Controller_2 Doc 065208 04 10 12 45 Dionex RFIC ER Kit Installation Instructions 8 5 Configuring the ICS 1000 1500 2000 Properties 1 In the Server Configuration program right click the ICS 1000 1500 2000 to open the system Properties dialog box Click the Options tab in the Properties dialog box In this example the system is a Dionex ICS 1500 Dionex ICS 1500 IC System Solvents State Devices Inject Valve Eror Levels Demo Chromatogram TTL inputs Trend Calibration Diagnostics General Options Signals HeadType amp Limits Installed Options WV Degas Link to ERC Controller F Column Heater ERC 10 Dionex ERC 10 Controller X Degas Mode C Always Off C Always On C Cycle On 0 120 sec Off 0 5940 sec Monitor Under Link to ERC Controller select the controller installed in the system Under Degas Mode verify that Monitor is selected Click OK Exit the Server Configuration program When asked whether to save the configuration changes click Save NOTE Go to Section 9 to check the Dionex RFIC ER Wellness panel settings 46 Doc 065208 04 10 12 Chromeleon Setup 8 6 Configuring the Dionex ICS 3000 DC Properties 1 Inthe Server Configuration program right click the DC to open the DC Proper
21. IonPac Analyte Trap Column anion column AAT ER2 cation column CAT ER2 When a sample is injected into the system it is important that the ions introduced in the sample do not enter the eluent bottle Analyte ions in the sample can increase the background conductivity of the eluent while counterions can affect the recovery of certain analytes The ER2 traps all analyte ions from the eluent stream allowing the eluent to be regenerated for reuse The anion ER2 contains high capacity microporous anion exchange resin in the bicarbonate form The cation ER2 contains high capacity microporous cation exchange resin in the hydronium form s The ER Controller ERC 10 contains two ER3 Dionex IonPac Catalyzer Columns a gas monitor and connecting tubing During the suppression regeneration process water is electrolyzed to hydrogen and oxygen gases In order to maintain the volume of the eluent these gases need to be converted back to water The ER3a column provides a delay that allows the most electroactive by products to decay the ER3b column has a platinum catalyst to complete the breakdown of any remaining electrolytic by products and to reform water from the oxygen and hydrogen gases The gas monitor tracks the volume of gas exiting the ER3 columns If this volume exceeds the predefined safety limit the ERC 10 shuts down the pump e The 4 liter eluent bottle has a dual line cap The outlet line is directed from near the top of the eluent
22. ady for operation e ICS 3000 systems only Refer to the table below for the ICS 3000 DC temperature control ranges when the Dionex RFIC ER mode is enabled ICS 3000 DC without separate column 10 to 30 C temperature control ICS 3000 DC with separate column Upper compartment 10 to 30 C temperature control Column compartment 10 to 70 C 12 Dionex RFIC ER System Maintenance 12 1 Replacing the Eluent When to replace the eluent The Dionex RFIC ER Wellness feature in Tue Chromeleon monitors eluent use and the Operation Time Ohows remaining Time total volume of sample injected into the system When the limit for either of these RemainingTime 672 hows parameters is reached an Audit Trail Volume Injected 0 message will inform you that it is time to Remaining Inj Volume replace the eluent Remaining Inj Volume 20000 You can check the life expectancy of the eluent at any time by reviewing the status information on the Dionex RFIC ER Remaining injections 0 Wellness panel For details about the KS Wellness panel parameters see the table below Injections Made 0 Doc 065208 04 10 12 53 Dionex RFIC ER Kit Installation Instructions Operation Time Remaining Time Volume Injected Remaining Inj Volume Injections Made Remaining Injections Reset EC The number of hours the eluent has been in use The number of hours until the eluent expires After 672 hours 28 days the elu
23. ard the column Remove the column plugs from the new ER2 column Connect the cell outlet tubing to the inlet of the new ER2 column Connect the suppressor Regen In port tubing to the outlet of the new ER2 column Mount the ER2 column on the ER2 column bracket clip on the suppressor Disconnect the ERC 10 from the suppressor Regen Out port Connect a short piece of tubing to the Regen Out port and direct this tubing to a waste container This is a temporary connection Select the standard flow rate and current settings for the application Turn on the pump and suppressor and begin pumping the operating eluent through the ER2 column for 30 minutes If the pump stops running and the Audit Trail displays the Pump stopped because the total gas volume has exceeded the limit message follow these steps a Under ERC 10 Controller on the Dionex RFIC ER Wellness panel click Reset Gas Volume This resets the Gas Volume to 0 00 mL b Restart the pump and suppressor and monitor the Gas Volume reading If the value begins to climb toward 0 60 mL click Reboot ERC 10 under ERC 10 Controller This disables the gas monitor for three hours c If after three hours the Gas Volume reading is still increasing shut down the system and contact Thermo Fisher Scientific for troubleshooting assistance 60 Doc 065208 04 10 12 Dionex RFIC ER System Maintenance IMPORTANT Do not operate the system with the gas monitor deactivated
24. ate Devices Inject Valve EnorLevels Demo Chromatogram TTLinputs Trend Calibration Diagnostics General Options Signals HeadType amp Limits Installed Options W Degas Link to ERC Controller I Column Heater ERC 10 Dionex ERC 10 Controller Degas Mode C Always Off C Always On C Cycle On 0 120 sec Off 0 5940 sec Monitor Under Degas Mode select Monitor 4 Click OK Exit the Server Configuration program When asked whether to save the configuration changes click Save 64 Doc 065208 04 10 12
25. bing the ER1 Column for Conditioning 1 Disconnect the tubing labeled TO INJ VALVE IN P from port P 2 on the injection valve Connect the tubing to a 10 32 union P N 042627 see Figure 5 Figure 5 Connecting the ER1 Column for Conditioning 2 Connect one end of the PUMP TO ER1 IN tubing to the 10 32 union Connect the other end of the tubing to the ER1 column inlet 12 Doc 065208 04 10 12 Initial Setup for ICS 1000 1500 2000 Systems 3 Connect the ER1 OUT TO INJ VALVE IN tubing to the ER1 column outlet Direct the free end of the tubing to a small waste container see Figure 6 Figure 6 Completing Connections for ER1 Column Conditioning 6 4 Conditioning the ER1 Column 1 Verify that the suppressor is off To avoid damaging the suppressor always turn off the suppressor before conditioning the ER1 column Although the pump flow is on during conditioning no flow will reach the suppressor 2 Set the pump flow rate to 2 0 mL min and turn on the pump 3 Rinse the ERT column with eluent for 30 minutes Make sure the suppressor is not activated during this time 4 After 30 minutes turn off the pump Doc 065208 04 10 12 13 Dionex RFIC ER Kit Installation Instructions 6 5 Installing the ER1 Column 1 Mount the ER1 column with the inlet end to the right on the ER1 column bracket clip 2 Connect the TO INJ VALVE IN P tubing to port P 2 on the injection valv
26. bottle while the inlet line is directed to the bottom of the eluent bottle Thus eluent is pumped from the top of the bottle and returned to the bottom Two gas vents in the eluent bottle cap ensure that any gases produced by the suppressor are safely vented to the atmosphere The eluent bottle and cap provided in the Dionex RFIC ER Installation Kit are designed specifically for use with Dionex RFIC ER systems Do not operate the system with any other eluent bottle or cap CAUTION La bouteille d luant et son bouchon fournis dans le kit d installation du syst me Dionex RFIC ER sont con us sp cifiquement pour les syst mes Dionex RFIC ER N utilisez aucun autre type de bouteille ou MISEENGARDE de bouchon avec le syst me Dionex RFIC ER Die Eluentenflasche und der Deckel aus dem Dionex RFIC ER Installationskit sind speziell fur die Verwendung mit einem Dionex RFIC ER System bestimmt Verwenden Sie mit diesem System keine VORSICHT anderen Eluentenflaschen oder Deckel Doc 065208 04 10 12 7 Dionex RFIC ER Kit Installation Instructions 4 Unpacking and Inspection Unpack the shipping containers and verify that all items listed on the packing list are on hand The Dionex RFIC ER Anion Startup Kit P N 067797 consists of e Dionex RFIC ER Anion Installation Kit P N 067786 e Dionex RFIC ER Anion Consumables Kit P N 067791 The Dionex RFIC ER Cation Startup Kit P N 067798 consists of e Dionex RFIC ER Cation Installation Kit P
27. e see Figure 7 Figure 7 Connecting the ER1 Column to the Injection Valve 14 Doc 065208 04 10 12 Initial Setup for ICS 1000 1500 2000 Systems 3 Tuck the tubing behind the ERT column and route the two eluent lines behind the tubing chase on the left of the system see Figure 8 Figure 8 Routing the ER1 Tubing Through the Tubing Chase 6 6 Installing the ER2 Column 1 Remove the backing tape from the Analyte Trap Column ER2 bracket P N 067297 see Figure 9 Backing Tape Figure 9 ER2 Column Bracket Doc 065208 04 10 12 15 Dionex RFIC ER Kit Installation Instructions 2 Press the bracket firmly into place on the suppressor see Figure 10 If necessary you can remove and then replace the bracket by separating the dual lock Velcro tape Figure 10 Attaching the ER2 Column Bracket to the Suppressor 16 Doc 065208 04 10 12 Initial Setup for ICS 1000 1500 2000 Systems Disconnect the black PEEK tubing that connects the Cell Out port of the detector cell to the suppressor Regen In port Connect the Cell Out port of the detector cell to the ER2 column inlet using the CELL OUT TO ER2 IN tubing see Figure 11 Figure 11 Connecting the ER2 Column to the Detector Cell Doc 065208 04 10 12 17 Dionex RFIC ER Kit Installation Instructions 5 Connect the ER2 column outlet to the suppressor Regen In port usin
28. e 20000 Injections Made 0 Remaining Injections 0 Reset EC Remaining Inj Volume 130000 ERC 10 Controller G Condition Suppressor Injections Made 0 Connected iv Mode Operation Gas Volume 0 00 ml Remaining Injections Reset ER2 Reboot ERC 10 Reset Gas Volume ER2 column Remaining Inj Volume Volume Injected 0 a If a new or freshly regenerated SRS 300 Suppressor is installed in the Dionex RFIC ER system go on to Section 10 to condition the suppressor If the installed suppressor is not new or freshly regenerated this completes the Dionex RFIC ER upgrade procedure Before starting operation review the operating guidelines in Section 11 50 Doc 065208 04 10 12 Conditioning the Suppressor 10 Conditioning the Suppressor A new or freshly regenerated SRS 300 Suppressor must be conditioned for 16 hours before it is ready for operation in the recycled eluent mode During this conditioning procedure the SRS 300 screens and membranes are equilibrated into the state required for compatibility with Dionex RFIC ER operation 1 Turn on the pump and suppressor at the flow rate and current settings recommended for the installed separator column see Section 6 8 or Section 7 6 2 If the Wellness panel is not already displayed click the Dionex RFIC ER Wellness tab on the panel tabset in Chromeleon Under ERC 10 Controller click Condition Suppressor to start the conditioning procedure 3 While conditioning
29. e Cell Out port of the detector cell to the suppressor Regen In port Doc 065208 04 10 12 31 Dionex RFIC ER Kit Installation Instructions 4 Connect the ER2 column inlet to the Cell Out port of the detector cell using the CELL OUT TO ER2 IN tubing see Figure 27 Figure 27 Connecting the ER2 Column to the Detector Cell 32 Doc 065208 04 10 12 Initial Setup for ICS 3000 Systems 5 Connect the ER2 column outlet to the suppressor Regen In port using the ER2 OUT TO SRS REGEN IN tubing Attach the ER2 column to the ER2 bracket on the suppressor see Figure 28 Figure 28 Attaching the ER2 Column to the Suppressor Doc 065208 04 10 12 33 Dionex RFIC ER Kit Installation Instructions 6 Place the ERC 10 in the Eluent Organizer EO 7 Connect the ERC 10 inlet tubing to the SRS REGEN OUT TO ER3 IN tubing using the supplied union 8 Connect the extended ERC 10 inlet tubing to the suppressor Regen Out port see Figure 29 Figure 29 Connecting the ERC 10 to the Suppressor 34 Doc 065208 04 10 12 Initial Setup for ICS 3000 Systems 9 Route the ERC 10 inlet tubing through the top right tubing chase to the DP SP and then up through the top tubing chase of the pump see Figure 30 Figure 30 Routing the ERC 10 Inlet Tubing Through System Tubing Chases Doc 065208 04 10 12 35 Dionex RFIC ER Kit Installation Instructions
30. e flow rate and current see the following table Separator Column Recommended Maximum Suppressor Flow Rate Current AS4A SC Anion 2 0 mL min 27mA Separator AS9 HC Anion 1 0 mL min 45 mA Separator AS12A Anion 1 5 mL min 25 mA Separator AS14 Anion Separator 1 2 mL min 25 mA AS14A Anion 1 0 mL min 43 mA Separator AS22 Anion Separator 1 2 mL min 31 mA AS23 Anion Separator 1 0 mL min 25 mA CS12A Cation 1 0 mL min 59 mA Separator CS16 Cation Separator 1 0 mL min 88 mA 2 Begin operating the system for 3 hours i e with the system not operating in the recycled mode of operation During this time periodically check all tubing connections for leaks Tighten any connections that leak After 1 5 hours check the eluent bottle If the eluent level is below the minimum fill mark see Figure 3 turn off the system refill the eluent bottle and restart the system If the eluent level falls below the minimum fill mark the outlet tubing IMPORTANT A may not reach the fluid level and the pump may lose prime 3 After 3 hours of total operation time shut down the system 4 Disconnect the waste line from the ERC 10 outlet 22 Doc 065208 04 10 12 Initial Setup for ICS 1000 1500 2000 Systems 5 Disconnect the green ERC 10 inlet tubing from the suppressor Regen Out port and allow the fluid to drain into a waste container 6 Connect the ERC 10 inlet tubing to a 1 4 28 union P N 039056 luer adapter P N 024305 and 10
31. em demo mode activa 1 32 26 PM Initializing driver Dionex Default USB 1 32 26 PM Initializing driver Dionex AS Autosampler vi 1 32 26 PM Initializing driver Dionex ICS 1500 IC System 1 32 34 PM User jmadden from JMADDENLAP has connected sug Configuration to this server For Help press F1 Ok 6 Right click the icon for the system or DC to be upgraded Doc 065208 04 10 12 41 Dionex RFIC ER Kit Installation Instructions The Properties dialog box for the device appears In this example the device is a Dionex ICS 1500 Dionex ICS 1500 IC System Solvents State Devices Inject Valve ErorLevels Demo Chromatogram TTLinputs Trend Calibration Diagnostics General Options Sonde Head Type amp Limits Device Name Mode Live Module SerialNo 04030467 D Moduleware version Download C Virtual Use Live mode to control a physical module connected to the server Virtual mode can be used to demonstrate the software support for the module and allows one to create methods and panels without having a physical module attached to the server 7 On the General tab check the Moduleware version If the correct version of Moduleware is installed see Section 8 1 go directly to Section 8 4 to configure the ERC 10 properties To update the Moduleware click Download The following message will appear Communication to the device will be stopped for configuration It will affect a
32. ent will expire and must be replaced Note This time limitation is independent of pump operation the eluent will expire after 672 hours 28 days even if the system is not operated during this time The volume of sample injected since the last eluent change When the Volume Injected is 20 000 uL the eluent has expired and must be replaced Note Repeated sample injections cause eluent dilution Operating the system with diluted eluent will cause excessive retention time drift for details see Section 11 The volume of sample that can be injected before the eluent expires Resetting the eluent counter resets the Remaining Inj Volume to zero also When you start the next injection the Remaining Inj Volume will be updated to the correct value Important To ensure the accuracy of the Remaining Inj Volume be sure to enter the correct injection volume in the Inj Vol column of the sequence table The number of sample injections since the last eluent change The number of samples that can be injected before the eluent expires Clicking Reset EC resets the eluent counter resetting the Operation Time and Remaining Inj Volume to zero Click Reset EC only after replacing the eluent How to replace the eluent 1 Check that the pump and suppressor are turned off 2 Disconnect the Eluent Bottle In and Eluent Bottle Out tubing from the pump and the ERC 10 54 Doc 065208 04 10 12 Dionex RFIC ER System Maintenance
33. for an extended period of time 9 10 11 12 After rinsing the ER2 column for 30 minutes turn off the pump Disconnect the temporary waste tubing from the suppressor Regen Out port and reconnect the ERC 10 to the port Under ER2 Column on the Dionex RFIC ER Wellness panel click Reset ER2 Restart the pump and suppressor The system is now ready for routine operation How to regenerate the ER2 column 1 2 Check that the pump and suppressor are turned off Remove the ER2 column from the ER2 column bracket clip on the suppressor Disconnect the cell outlet tubing from the inlet of the ER2 column disconnect the suppressor Regen In tubing from the outlet of the ER2 column If the ER2 column is an anion column follow these steps a Prepare fresh 0 5 M sodium bicarbonate solution Use either reagent grade sodium bicarbonate or undiluted Thermo Scientific Sodium Bicarbonate Concentrate 0 5 Molar P N 037163 b Pump 200 mL of 0 5 M sodium bicarbonate solution through the ER2 column Use either the Trap Column Suppressor Cleanup Kit P N 059659 or an accessory pump at 5 0 mL min Direct the column effluent to a waste container c Pump 40 mL of deionized water through the ER2 column Use one of the following the Trap Column Suppressor Cleanup Kit P N 059659 an accessory pump at 5 0 mL min or a syringe Direct the column effluent to a waste container d Goon to Section 6 Doc 065208 04 10 12
34. g the ER2 OUT TO SRS REGEN IN tubing Mount the ER2 column on the ER2 column bracket on the suppressor see Figure 12 KZ CC Figure 12 Attaching the ER2 Column to the Suppressor 18 Doc 065208 04 10 12 Initial Setup for ICS 1000 1500 2000 Systems 6 7 Installing the ERC 10 1 Place the ERC 10 in the left hand Dionex EluGen cartridge holder on the top cover of the ICS 1000 1500 2000 see Figure 13 Figure 13 ERC 10 in EluGen Cartridge Holder 2 Route the green ERC 10 inlet tubing down the tubing chase to the front of the system see Figure 14 Figure 14 Routing the ERC 10 Inlet Tubing Through the Tubing Chase Doc 065208 04 10 12 19 Dionex RFIC ER Kit Installation Instructions 3 Connect the green ERC 10 inlet tubing to the suppressor Regen Out port see Figure 15 KA E 7 Figure 15 Connecting the ERC 10 to the Suppressor 20 Doc 065208 04 10 12 Initial Setup for ICS 1000 1500 2000 Systems 4 Connect waste tubing to the ERC 10 outlet see Figure 16 Direct the free end of the waste line to a small waste container Figure 16 Connecting the ERC 10 Waste Line IMPORTANT Do not connect the ERC 10 USB cable to the ICS 1000 1500 2000 until instructed to do so Doc 065208 04 10 12 21 Dionex RFIC ER Kit Installation Instructions 6 8 Testing for Leaks 1 Turn on the pump and suppressor at the appropriat
35. in excessive volumes whether due to a large number of small injections or a smaller number of injections with large sample loops will dilute the eluent and cause unacceptable retention time drift Chromeleon monitors the sample volume injected and displays a warning to replace the eluent when the limit is approached When running sequences the Dionex RFIC ER Wellness feature uses the current injection volume to monitor the volume of sample that is injected into the system and to calculate the number of injections that remain Before the first injection Chromeleon may be unable to calculate the number of remaining injections however the affected eluent and trap column counters on the Dionex RFIC ER Wellness panel will correct themselves when the first injection is made 52 Doc 065208 04 10 12 Dionex RFIC ER System Maintenance e When injecting samples manually or with a Dionex AS40 Automated Sampler be sure the injection volume specified in the Chromeleon sequence matches the sample loop size IMPORTANT Entering the wrong injection volume in the sequence will impair the accuracy of the eluent and trap column counters on the Dionex RFIC ER Wellness panel this will impair proper operation of the Dionex RFIC ER system e Itis not necessary to re equilibrate the system unless you replace the eluent or one of the trap columns Because the Dionex RFIC ER system remains on continuously the system is always equilibrated and re
36. is underway periodically check all tubing connections for leaks tighten any connections that leak To monitor the status of the conditioning procedure check the Remaining Time display under Eluent If the pump unexpectedly stops running and the Audit Trail displays the Pump stopped due to RFIC message follow these steps to resume the conditioning procedure a Under ERC 10 Controller on the Dionex RFIC ER Wellness panel click Reset Gas Volume to enable the pump again b Under ERC 10 Controller click Reboot ERC 10 to prevent the ERC 10 from deactivating the pump for 3 hours 4 When the conditioning procedure finishes the system automatically shuts down and Chromeleon displays the following message in the Audit Trail The eluent has expired and needs to be replaced now The pump has been shut down to protect the guard and analytical columns 5 Dispose of the used eluent Prepare 4 liters of fresh eluent and add eluent up to the maximum fill mark on the bottle see Figure 3 or Figure 20 6 Connect a waste line to the ERC 10 outlet Operate the system for 30 minutes to flush out the expired eluent 7 After 30 minutes turn off the pump and disconnect the waste line from the ERC 10 outlet Reconnect the Eluent Bottle In tubing to the ERC 10 outlet Doc 065208 04 10 12 51 Dionex RFIC ER Kit Installation Instructions 11 The Dionex RFIC ER system is now ready for up to four weeks of nonstop operation
37. n it is time to replace the column The ER1 column cannot be regenerated Never operate an ER1 column beyond its expiration this may result in irreversible loss of capacity to the guard and analytical columns You can check the life expectancy of the ER1 column at any time by reviewing the status information on the Dionex RFIC ER Wellness panel For details about the Wellness panel parameters see the table below ER column Capacity Consumed Capacity Remaining Remaining 0 0 100 0 56 Doc 065208 04 10 12 Dionex RFIC ER System Maintenance Capacity The percentage of the ER1 column that has been Consumed consumed When the Capacity Consumed is 100 the ERT column has expired and must be replaced Capacity The percentage of the ER1 column that is remaining Remaining When the Capacity Consumed is 0 the ERT column has expired and must be replaced Reset ER1 Clicking Reset ER1 resets the ERT column counters resetting the parameters above to 0 Click Reset ER1 only after replacing the ERT column How to replace the ER1 column 1 2 St Check that the pump and suppressor are turned off Remove the old ERT column from the ER1 column bracket clip Disconnect the pump tubing from the inlet of the old ER1 column disconnect the tubing labeled TO INJ VALVE IN P from the outlet of the old ER1 column Discard the column Remove the column plugs from the new ERT column
38. nt even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Revision history Revision 01 released February 2008 Revision 02 released March 2008 Revision 03 released January 2012 Revision 04 released October 2012 For Research Use Only Not for use in diagnostic procedures Contents Dionex RFIC ER Startup Kit Installation Instructions 1 Introduction 3 066 4a bo ent ERUN SEN ad ew ed bad eee ded 1 2 Safety Information 0 0 eee eee eens 2 2 1 Safety Messages 2 2 2 BRC TO Labels A wee r tae he a eds Art Ser dae ae 4 2 3 ERC 10 Specifications 5 3 Functional Description 6 4 Unpacking and Inspection 8 5 Procedure OvervieW 9 6 Initial Setup for Dioenx ICS 1000 1500 2000 Systems 10 6 1 Installing the ERT Column Bracket Clip 10 6 2 Installing the Eluent Bol 11 6 3 Plumbing the PRT Column for Conditioning 12 6 4 Conditioning the ERT Column 13 6 5 Installing the ERT Column 14 6 6 Installing the ER2 Column 15 6 7 Installing the ERC IO 19 6 8 Testing for Leaks aici ei oa diaud eh EA E Ne e 22 7 Initial Setup for ICS 3000 Systems 0 0
39. on to certain information They alert users to an unexpected result of an action suggest how to optimize instrument performance etc Doc 065208 04 10 12 3 Dionex RFIC ER Kit Installation Instructions 2 2 ERC 10 Labels The TUV GS and cTUVus Mark safety labels and the CE Mark label on the ERC 10 indicate that the ERC 10 is in compliance with the following standards and directives EMC Susceptibility and Immunity e EN 61326 1997 A1 1998 A2 2001 e Low Voltage Equipment Directive 73 23 EEC e EMC Directive 89 336 EEC Safety e EN 61010 1 2001 e CAN CSA C22 2 No 61010 1 2004 e UL 61010 1 2004 These symbols appear on the ERC 10 or on ERC 10 labels om Alternating current D Protective conductor terminal Power supply is on Indicates a potential hazard Refer to the user manual for an Z explanation of the hazard and how to proceed Doc 065208 04 10 12 Safety Information 2 3 ERC 10 Specifications Main Power Operating Temperature Operating Humidity Dimensions Weight 5 volts DC Typical input power 250 mW Typical line draw 5 mA 4 to 40 C 40 to 104 F 5 to 95 relative humidity noncondensing Height 18 73 cm 7 375 in Width 5 72 cm 2 25 in Depth 3 45 cm 1 375 in 0 38 kg 0 83 Ib Doc 065208 04 10 12 Dionex RFIC ER Kit Installation Instructions 3 Functional Description This section presents a brief overview of key Dionex RFIC ER components
40. ons for the ER1 Column 7 4 Conditioning the ER1 Column 1 Verify that the suppressor is off To avoid damaging the suppressor always turn off the suppressor before conditioning the ER1 column Although the pump flow is on during conditioning no flow will reach the suppressor 2 3 Set the pump flow rate to 2 0 mL min and turn on the pump Rinse the ER1 column with eluent for 30 minutes Make sure the suppressor is not activated during this time After 30 minutes turn off the pump 28 Doc 065208 04 10 12 Initial Setup for ICS 3000 Systems 5 Connect the tubing labeled TO INJ VALVE IN P to the heat exchanger see Figure 24 NOTE Tuck the tubing into the tubing clips at the bottom of the DC Figure 24 Connecting the ER1 Column to the Heat Exchanger Doc 065208 04 10 12 29 Dionex RFIC ER Kit Installation Instructions 7 5 Installing the ER2 Column 1 Remove the backing tape from the Analyte Trap Column ER2 bracket P N 067297 see Figure 25 Backing Tape Figure 25 ER2 Column Bracket 30 Doc 065208 04 10 12 Initial Setup for ICS 3000 Systems 2 Press the bracket firmly into place on the suppressor see Figure 26 If necessary you can remove and then replace the bracket by separating the dual lock Velcro tape Figure 26 Attaching the ER2 Column Bracket to the Suppressor 3 Disconnect the black PEEK tubing that connects th
41. ps running and the Audit Trail displays the Pump stopped because the total gas volume has exceeded the limit message follow these steps a Under ER Controller on the Dionex RFIC ER Wellness panel click Reset Gas Volume This resets the Gas Volume to 0 00 mL Doc 065208 04 10 12 55 Dionex RFIC ER Kit Installation Instructions 12 2 b Restart the pump and suppressor and monitor the Gas Volume reading If the value begins to climb toward 0 60 mL click Reboot ERC 10 under ER Controller This disables the gas monitor for three hours c If after three hours the Gas Volume reading is still increasing shut down the system and contact Thermo Fisher Scientific for troubleshooting assistance Do not operate the system with the gas monitor deactivated for an extended period of time 11 After 30 minutes of flushing eluent from the system turn off the pump 12 Disconnect the waste line from the ERC 10 outlet Connect the outlet of the ERC 10 to the Eluent Bottle In tubing 13 Check that the eluent level is above the minimum fill mark on the eluent bottle see Figure 3 or Figure 20 If necessary add more eluent to the bottle 14 Restart the pump and suppressor The system is now ready for routine operation Replacing the ER1 Column When to replace the ER1 column The Dionex RFIC ER Wellness feature in Chromeleon monitors the use of the ER1 column An Audit Trail message will inform you whe
42. tains the Dionex RFIC ER system and select Edit gt Add Device The Add device to timebase dialog box appears gt Add device to timebase Timebase Name System_1 Manufacturers D ionex IC ICS 3000 Systems IC Modules HPLC UkiMate 3000 HPLC Summit Systems HPLC Autopurification Systems HPLC Gynkotek Systems HPLC Modules Pack hd M eee Capillary Nano LC Modules gt 1 25 IC System Se C254 IC System S ICS 1000 IC System ICS 1500 IC System ICS 2000 IC System se ICS 30 IC System 5 Inthe Manufacturers list box select Dionex IC Integrated Systems 6 In the Devices list box select ERC 10 Controller 7 Click OK to add the ERC 10 to the timebase 44 Doc 065208 04 10 12 Chromeleon Setup The ERC 10 Properties dialog box appears Dionex ERC 10 Controller General Options Eror Levels Device Name Controlled Mode e ModueSeidINc d Dod Use Live mode to control a physical module connected to the server Virtual mode can be used to demonstrate the software support for the module and allows one to create methods and panels without having a physical module attached to the server 8 On the General tab make sure that the Live mode is selected 9 If more than one ERC 10 is connected to the server select the correct ERC 10 from the Module Serial No list 10 Click OK NOTE If the Dionex RFIC ER system is a ICS 1000 1500 2000 go to Section 8 5 If the Dionex RF
43. tes that the function or process of the instrument may be IMPORTANT impaired Operation does not constitute a hazard Doc 065208 04 10 12 Safety Information Messages d avertissement en francais Signale une situation de danger imm diat qui si elle n est pas vit e entra nera des blessures graves a mortelles gt DANGER Signale une situation de danger potentiel qui si elle n est pas vit e pourrait entrainer des blessures graves a mortelles gt AVERTISSEMENT Signale une situation de danger potentiel qui si elle n est pas vit e pourrait entra ner des blessures mineures mod r es galement utilis pour signaler une situation ou une pratique qui pourrait MISEEN GARDE gravement endommager l instrument mais qui n entrainera pas de blessures gt Warnhinweise in Deutsch Bedeutet unmittelbare Gefahr MiBachtung kann zum Tod oder schwerwiegenden Verletzungen f hren gt ACHTUNG Bedeutet eine m gliche Gef hrdung Mi achtung kann zum Tod oder schwerwiegenden Verletzungen f hren gt WARNUNG Bedeutet eine m gliche Gef hrdung Mi achtung kann zu kleineren oder mittelschweren Verletzungen f hren Wird auch verwendet wenn eine Situation zu schweren Sch den am Ger t f hren kann jedoch VORSICHT keine Verletzungsgefahr besteht gt Informational messages also appear throughout this manual These are labeled NOTE and are in bold type NOTE NOTES call attenti
44. the atmosphere N obturez pas les vents du bouchon de la bouteille d luant En cas de d faillance de l ERC 10 les vents doivent pouvoir vacuer vers l atmosph re l hydrog ne et oxyg ne produits par le suppresseur lectrolytique 26 Doc 065208 04 10 12 Initial Setup for ICS 3000 Systems Blockieren Sie keinesfalls die ffnungen im Deckel der Eluentenflasche Wenn der ERC 10 nicht korrekt arbeitet m ssen die vom elektrolytischen Supressor erzeugten Wasserstoff und VORSICHT Sauerstoffgase Uber diese ffnungen in die Atmosph re gelangen k nnen 4 Connect the Eluent Bottle Out tubing to the pump inlet line 5 Prime the pump 6 Make sure the suppressor has been hydrated as instructed in the SRS 300 QuickStart Suppressor manuals are provided on the Thermo Scientific Reference Library DVD P N 055405 7 3 Plumbing the ER1 Column for Conditioning L Disconnect the static mixer from the DP SP outlet and connect it to the ER1 column inlet see Figure 22 Figure 22 Connecting the ER1 Column to the Dionex ICS 3000 Pump Doc 065208 04 10 12 27 Dionex RFIC ER Kit Installation Instructions 2 Connect the ER OUT TO INJ VALVE IN tubing to the ERT column outlet and direct the free end of the tubing to a small waste container Mount the ER1 with the inlet end to the right on the ER1 column bracket clip see Figure 23 Figure 23 Conditioning Connecti
45. ties dialog box 2 Click the Suppressors tab in the Properties dialog box Dionex DC Detector Chromatography General Detectors High Pressure Valves Low Pressure Valves TTLs Relays AC Thermal Controls Suppressors TTL inputs Enor Levels Calibration Name Timebase Wi Suppressorl System_2 Suppressor 1 C Suppressor2 System_2 Suppressor 2 Deactivate check boxes to remove unused devices from Online Hit F2 to edit selection or double click it 3 Double click the suppressor installed in the Dionex RFIC ER system and press F2 Doc 065208 04 10 12 47 Dionex RFIC ER Kit Installation Instructions The Device Configuration dialog box appears Device Configuration Device Name Suppress Timebase AFIC ER Link to Pump Pump_1 Dionex DP Dual Pump v MV Enable RFIC ER Description Assign a unique symbolic name to the device x Ge 4 Select the Enable Dionex RFIC ER check box and click OK 48 Doc 065208 04 10 12 Chromeleon Setup 5 Right click the pump in the Dionex RFIC ER system to open the pump Properties dialog box In this example the pump is a DP Dionex DP Dual Pump Top Solvents Relays Inputs Calibration Diagnostics EnorLevels Trend General Devices Bottom Pump Bottom Solvents TopPump Device Name DP Top Pump Pump_2 on RFIC ER M Link to ERC Controller IERC 10 Dionex ERC 10 Bottom Pump Pump_1 on RFIC ER M Link to ERC
46. to normal operation 1 Click the Dionex RFIC ER Wellness tab on the panel tabset in Chromeleon Check the controls under ERC 10 Controller ERC 10 Controller If the Connected fv Condition Suppressor Gas Volume is 0 60 mL or Mode RS Gas volume 0 60 mi higher or if the Mode is Gas Volume Error it indicates Reboot ERC 10 that the volume of gas exceeds the allowable limit Click the Reset Gas Volume button to reset the Gas Volume and return the Mode to Operation Restart the pump and suppressor and monitor the Gas Volume If the reading begins to climb toward 0 60 mL click Reboot ERC 10 This disables the gas monitor for three hours After three hours check the Dionex RFIC ER Wellness panel again If the Gas Volume reading is still increasing and the Mode is Gas Volume Error shut down the system and contact Thermo Fisher Scientific for troubleshooting assistance 13 2 Unstable Retention Times ICS 1000 1500 2000 only Unstable retention times may indicate that the vacuum degas assembly is not operating in Monitor mode To select the correct degas mode 1 In the Server Configuration program right click the ICS 1000 1500 2000 to open the system Properties dialog box Doc 065208 04 10 12 63 Dionex RFIC ER Kit Installation Instructions 2 Click the Options tab in the Properties dialog box In this example the system is a Dionex ICS 1500 Dionex ICS 1500 IC System Solvents St
47. ver is running idle Quit Monitor SerialNo 21905 4 Start the Chromeleon Server Configuration program Select Start gt All Programs or Programs depending on the operating system gt Chromeleon gt Server Configuration 40 Doc 065208 04 10 12 Chromeleon Setup 5 Expand the contents of the timebase that includes the system to be upgraded by clicking the icon next to the timebase 2 Server Configuration Chromeleon Server Configuration File Edit View Server Help cE ts ael Ag OJR Alv E IMADDENLAP Messages Server JMADDENLAP Normal P Sharable Devices z G JMADDENLAP ICS5 1500 Server JMADDENLAP connected g ke 4 32 25 PM CmServer Chromeleon Serer Version 6 80 SP1 Build S Dionex IC5 1500 IC System 14 2006 Copyright Co 1994 2006 Dionex Corporation All rights re 1 32 25 PM SER_IO Dionex Serial Interface Driver Version 6 80 B Copyright c 1994 2006 Dionex Corporation All rights reserved 41 32 26 PM Chromeleon Default USB Driver Version 6 80 SP2 By Beta Copyright c 19942006 Dionex Corporation All rights resesi 1 32 26 PM AS Chromeleon AS Autosampler Driver Version 6 80 Sk 2244 Beta Copyright c 1994 2006 Dionex Corporation All righ 1 32 26 PM Driver Dionex AS Autosampler demo mode activated 1 32 26 PM ICS 1500 Chromeleon ICS 1500 System Driver Version Build 2244 Beta Copyright c 1994 2006 Dionex Corporation All ri reserved 1 32 26 PM Driver Dionex ICS 1500 IC Syst
48. w cost alternative to Dionex RFIC Eluent Generation Dionex RFIC EG systems Rather than generating eluent electrolytically from water using an eluent generator with an electrolyte solution Dionex RFIC ER uses the recycled eluent mode of operation of the electrolytic suppressor to regenerate the starting eluent The starting eluent for the Dionex RFIC ER system is prepared manually as usual After suppression and regeneration the eluent passes through a series of purification columns before being returned to the eluent bottle With the closed loop RFIC ER system the original 4 liters of eluent can be used for up to four weeks of continuous operation The following Thermo Scientific Dionex products can be upgraded to Dionex RFIC ER systems e Dionex ICS 1000 Dionex ICS 1500 or Dionex ICS 2000 Ion Chromatography System e Dionex ICS 3000 Ion Chromatography System NOTE The SRS 300 Suppressor is the only Thermo Scientific Dionex suppressor compatible with Dionex RFIC ER To upgrade an existing IC system to a Dionex RFIC ER system order a kit e Dionex RFIC ER Anion Startup Kit P N 067797 e Dionex RFIC ER Cation Startup Kit P N 067798 Each Startup Kit includes one Installation Kit and one Consumables Kit Together these kits contain all the consumable and non consumable items required to upgrade an IC system to a Dionex RFIC ER system and operate the system nonstop for 6 to 12 months Doc 065208 04 10 12 1 Dionex RF
49. ystem go to Section 7 Doc 065208 04 10 12 9 Dionex RFIC ER Kit Installation Instructions 6 initial Setup for ICS 1000 1500 2000 Systems In order to ensure that the eluent returned to the eluent bottle is identical in composition to the starting eluent several components in the Dionex RFIC ER system must be equilibrated before initial operation This section describes the temporary connections required for equilibration 6 1 Installing the ER1 Column Bracket Clip 1 Verify that the ICS 1000 1500 2000 is set up as directed in the operator s manual The manual is included on the Thermo Scientific Reference Library DVD P N 055405 2 Install the ER1 column bracket clip P N 066992 on the ICS 1000 1500 2000 component mounting panel see Figure 2 Angle the clip slightly about 10 degrees clockwise from vertical to allow the ERT column to clear the suppressor tangs Figure 2 Installing the ER1 Column Bracket Clip 10 Doc 065208 04 10 12 Initial Setup for ICS 1000 1500 2000 Systems 6 2 Installing the Eluent Bottle 1 Prepare 4 liters of the eluent required for the application Always use ASTM Type I 18 megohm cm filtered deionized water when preparing eluent 2 Note the minimum and maximum fill marks on the Maxi Fill eluent bottle see Figure 3 Add wegen tj fresh eluent up to the maximum fill mark ES Minimum Fill Mark About 3 7 L Figure 3 Eluent

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