ARIAS Optimatrix™ 500 User Guide
Contents
1. The Reichert ARIAS 500 Instruction Manual Reichert 1 0 S INTRODUCTION zen 2 2 0 UNPACKINGELECTRICALCONSIDERATIONS ENVIRONMENTALCONDITIONS 0 cceeteeeetteeeeeeees 2 3 0 INSTRUMENTCOMPONENTS aiie 3 40 OPERATINGINSTRUCTIONS nneensnennnnnnnnennn 3 41 Start up and Basic Sample Analysis gt 3 42 _ Changing Mode and Channel r nenn 3 4 3 M NUODTIONS ensnneeensennnnnnnnnnnnnnnnnennnnnnnnnnnnnnn nn 3 44 Care and Cleaning Preparing the Prism Surface for Samples ceceeeeeseseeeeestteeesees 5 45 Applyingthe Sample and Taking Readings 5 5 0 CALIBRATION nenssesensnnnnennsnnnnnnnnnnnnnnnnnnnnnnnnnnn nen 6 6 0 WATERBATHATTACHMENT 0 ccceceeeeeeeseeeeeeees 6 6 1 Helpful Hints for Water Bath Circulator Use 7 7 0 OOMPUTERINTERFACE nunnnnseesssnennnennnnnnnnnnnnnnnnn 7 80 MONG ira 7 9 0 REFRACTOMETERUSE TECHNIQUES AND REFRACTIONPRINCIPLES urn mneennsnnnnennnnnnnnnnnnn 7 9 1 Sample Analysis Hints 0 r4440nn nennen 7 10 0 MISCELLANEOUS enessnnnnsnnnnnsnnnnnnnnnnnnnnnnnnnnn 8 11 0 SPEGIFIGATIONS ee 8 Explanation of Warning Symbols CAUTION Risk of electric shock CAUTION Replace with same type and rating fuse 5x20 800mA T250 AN CAUTION Refer to accompanying documents JINTrOQAUCUON The ARIAS 500 is a transmitted light refractomete2. an angled piece of clear plastic tubing Completely cover the surface making sure all the air pockets between the sample and the prism surface are removed Generally analyzing these samples at a higher temperature will lower the viscosity and provide easier analysis Carbonated beverages or samples having numerous air bubbles will not produce a sharp distinct shadow line It may be possible to remove air bubbles in some samples by placing the sample on the Measuring Prism and opening and closing the Illumination Prism two or three times prior to analysis Non Homogenous samples or samples having undissolved particulate matter may not produce a sharp distinct shadowline It may be necessary to filter some samples prior to analysis When in doubt the Automatic Reading mode should be used to eliminate operator bias variance Some highly colored samples may not produce a sharp distinct shadowline It may be necessary to dilute some samples prior to analysis When in doubt the Automatic Reading mode should be usedto eliminate operator bias variance Some samples may require awave length of light other than 589 nanometers Please contact a Reichert representative for further information regarding optional wavelengths Itis also advisable to keep water on the prism and to keep the prisms closed together This will help maintain thermal equilibrium and will also decrease the amount of time to reach thermal equilibrium when the instrument is first
3. contrast for the display screen When this option is selected pressing the UP or DOWN arrow keys will increase or decrease the display contrast 4 Precision This option allows the user to choose the readability of the result The user may select High precision to obtain readings to the fifth decimal place 0 00001 for refractive index and to the second decimal 0 01 place for percent solids readings The user may also choose Low precision to obtain readings to the fourth decimal place 0 0001 for refractive index and to the first decimal place 0 1 for percent solids readings ID This option allows the user to enter an identification for sample analysis results The user may SELECT either Edit ID or None Also if an ID has already been defined it will appear and may be chosen Choosing None will disable the ID option Choosing Edit ID will allow the user to either Enter New ID Delete ID or Exit If Enter New ID is selected the user will be prompted to enter an identification using the UP and DOWN arrow keys to change the value of the listed character within the cursor as well as the READ key to move the cursor from left to right Once the ID has been set press SELECT to return to the options screen within ID The user may enter up to 25 IDs Once all IDs have been entered select one from the ID option screen in the MAIN MENU Selecting the option None will disable the ID feature Once an ID has been selected the user may choose an
4. turned on when a water bath is attached and when samples are applied and removed To optimize shadowline quality and thus improve accuracy avoid trapping air bubbles within the sample between the lower Measuring Prism and upper Illumination Prism Bubbles in the sample should be visible through the face of the upper Illumination Prism 10 0 Miscellaneous The ARIAS features a new technology known as FLASH programming which allows software upgrading via computer connection through the serial port This allows the end user to load new versions of software in the field thus eliminating the need to send the instrument to a Reichert facility for repair when anew software version is released Please contact Reichert Analytical Products Division for more information 11 0 SPECIFICATIONS Models 1310499SA US 110 115V USAPowercord 1310499SA EU 220 240V European Powercord Universal 1310499SA UK 220 240V UK Powercord Automatic Read Range Shadowline within field of view Illumination 6 High intensity LEDs 589 nm wavelength filter also built in Display 1 75 Wx1 75 H 44 mm x44 mm Range 1 3000 to 1 7000 Readability 0 00001 Precision 0 00005 Automatic Mode 0 0001 Manual Mode The precision and accuracy of Refractive Index measurements are largely dependent upon operator skill laboratory equipment temperature control the accuracy of calibration standards chemical stability of sample and the physical charact
5. 6 4555 email ai reichert com www reichert com Reichert GmbH Hubertusstrasse 4 D 82229 Seefeld Germany Tel 49 8152 993530 ISO 9001 Certified Reichert 2003 Reichert Inc Depew NY USA 1310499SA 101 Rev D
6. Active Inactive to choose SELECT to toggle Figure 1 Choosing Advanced Options will allow the user to select from 3 additional options The Field of View Calibration option is more involved than the crosshair calibration in that it requires a minimum of three separate readings within the field of view for each calibration point This includes one with the shadowline near the top of the crosshairs one at the crosshair intersection and one near the bottom of the crosshairs as seen through the eyepiece To achieve better accuracy 5 to 7 points across the field are recommended The instrumenthas the capacity to store up to 250 field points spread between the 7 calibration points As with Crosshair calibration distilled water should be included inthe calibration routine as well as 1 6 additional calibration points Afull 7 point Field Of View calibration will obtain maximum accuracy for this instrument To editthe calibration standard data select Edit Data from the Advanced Options menuin the Calibration screen The user may edit the refractive index temperature coefficient and reference datum temperature for each standard Follow the on screen instructions once Edit Data has been selected and the standard to be modified has been chosen Editing any calibration standard will delete all calibration points associated with it The user will have to recalibrate with the new data for the instrumentto use that calibration point WAR
7. ELECT key untilan UP anda DOWN arrow appear in front of the ID Pressing the UP or DOWN arrow on the KEYPAD will allow the user to change the ID without having to access the MENU NOTE This feature will only function ifat least one ID has been entered into the unit To Change the Channel i e from SUCROSE ICUMSA to user programmed custom channels press the SELECT key untilan UP and a DOWN arrow appearin frontofthe Channel Name Pressing the UP or DOWN arrow on the KEYPAD will allow the user to change the channel without having to access the MENU NOTE This feature will only function ifat least one custom channel has been programmed into the unit 4 3 Menu Options To access the menu options screen simply press the MENU key Following are the user definable options available To access an option simply use the UP or DOWN arrow keys to highlight the desired parameter then press SELECT The parameter will then listthe optional settings below and to the right Using the UP and DOWN arrows scroll through the options and press SELECT when the parameter desired is highlighted Further on screen instructions and this instruction manual will walk you through any necessary procedures Calibration The instrument will allow the user to calibrate according tothe level of accuracy that best suits the needs of the application See SECTION 5 0 for detailed information regarding calibration Channel Custom channels may be programmed into the A
8. ID inthe normal reading screen by pressing the SELECT key until an UP anda DOWN arrow appear in front of the ID Pressing the UP or DOWN arrow on the KEYPAD will allow the user to change the ID to any which has been entered without going into the MENU Once a suitable ID has been selected a sample reading may be taken 4 4 Care and Cleaning Preparing the Prism Surface for Samples The ARIAS is a precision optical instrument As such itis essential that the instrumentis kept clean and well maintained Clean the Measuring Prism and Illumination Prism with distilled water diluted soapy water or alcohol dependent upon the sample being analyzed Rinse with distilled water and gently dry before applying a sample Residue left on the prism will affect resultant readings The prism surface should be cleaned with a solution which will remove sample residue Rinsing with distilled water as a final step is recommended to avoid film formation on the prism The prism surface may be damaged by abrasive materials Use caution when removing samples or drying the prism Thick sticky samples should be rinsed off with water or a suitable solvent to avoid wiping and possibly scratching the prism The sealant used between the prism and stainless steel block may be attacked by certain chemicals Reichert recommends avoiding strong sol vents such as N N Dimethylformamide or N N Dimethylacetamide cresols phenols and other tar acids Tetrahydrofuran and some l
9. NING Changing the fields in the Edit Data section for calibration standards must be done to match the values for the exact standard to be used Reichert has predefined these fields to match available calibration standards In particular do not modify Point 1 as this is set for distilled water and should remain constant throughout the world Modifying these fields incorrectly will result in miscalibration and will affect reading results Once calibration is complete select EXIT from the Calibration screen Note Routine standard analysis should be performed to ensure thata laboratory instrument is performing accurately Arefractometer isno exception Reichert cannot recommend specific calibration intervals howeverat minimuma daily water reading verification is desirable Please follow established laboratory protocol for calibration and performance verification intervals Typically recalibration with distilled water on a routine basis will be all that is necessary to maintain accuracy 6 0 Water Bath Attachment Certain testing applications may require the prism temperature to be maintained above or below ambient operating temperature Commercial water bath circulators with heating cooling or combined capabilities are generally available from laboratory instrument supply dealers Ifa water bath attachment is used be careful to avoid exposing the refractometer s prisms to thermal shock The ambient prism temperature should be raised or
10. RIAS Channel number One is based upon ICUMSA data converting refractive indices at various temperatures to their corresponding Percent Solids levels for sucrose solution concentration Percent Solids and Refractive Index Temperature Compensation is always based upon the conversion formulas programmed as the selected channel Non Temperature Compensated Refractive Index readings are independent of channel selection To program a custom channel for a specific product please contact a Reichert representative Once a custom channel is programmed it may be selected by pressing SELECT once Channel has been highlighted then using the UP or DOWN arrow keys until the desired channel is highlighted Pressing the SELECT key again will then enable that channel Percent Solids and Temperature Compensation will then be based upon the custom channel formula programmed Reading Method TheARIAS 500 derivesits name from the instruments unique ability to read a sample manually similar to a standard transmitted light refractometer as well as to take an automatic reading of the shadowline intersect value once it is within the crosshairs To select the Reading Method simply highlight this option and press SELECT To change to the Manual mode highlight Manual and press SELECT Touse the Automatic method highlight Auto and press SELECT The Automatic reading method will help eliminate operator error from shadowline intersect interpretation This can be a signifi
11. able contactfluid on the lower Measuring Prism having a refractive index higher than the desired sample Then place the solid sample down avoiding air bubbles The sample shouldbe placed atthe rear of the Measuring Prism Note Do not lower the illumination prism onto a solid sample as it may cause damage to the instrument Solid samples must be shaped and polished correctly in order to be read The size should be no more than 35mm Long X 12mm Wide X aminimum of 2mm high Also one side of the sample mustbe polished like glass as well as one of the perpendicular vertical faces on the long axis All other sides should be frosted The polished side willbe placed down onto the contact fluid while the vertical perpendicular polished side will be faced toward the light source 5 0 Calibration There are two modes that a user can choose from Crosshair and Field of View Typically a Crosshair calibration will be all that is required by most users A Field of View calibration involves more time to perform but may be necessary ifa Crosshair calibration does notyield automatic reading results that are deemed accurate To select acalibration type simply highlightthe option desired usingthe UP and DOWN arrowkeysandpress select To exit select Exit For all calibration options chosen the user is prompted step by step through a complete calibration Crosshair calibration is performed by using the Adjust Set Point alone or the Adjust Set Point combine
12. ac quer thinners can also deteriorate the seal over time or when used at elevated temperatures To determine if your samples or solvents may attack this seal please contact a Reichert representative for test glass cementsamples 4 5 Applying the Sample and Taking Readings Using a pipette or other applicator place the liquid sample onto the surface of the lower Measuring Prism Gently lower the upper Illumination Prism onto the sample A magnetic latch will hold the two prisms together Looking through the eyepiece rotate the Shadowline Adjustment Knob until the shadowline the portion where the light and dark fields intersect is centered in the crosshairs Press the READ button The result will be displayed on the main display along with all other pertinentinformation such as mode reading style channel date time and temperature Note Ifthe Automatic Reading Method has been selected itis not necessary toalign the shadowline with the center of the crosshairs Simply move the shadowline anywhere within the crosshairs range and press READ to take an automatic reading of the sample The mode may be changed by entering the menu and selecting the desired mode Note once a reading has been taken changing the mode will allow the userto see whatthe result of that reading would have been in any other mode The ARIAS can also read solid samples such as glass and transparent plastics in the manual reading mode Simply apply a drop of a suit
13. ace a computer with the instrument a straight through RS232 cable with 9 pin connecior will be needed These standard cables are available at most electronics or computer stores Simply connect one endto the port on the refractometer and the other end to the COM port onthe computer The COM port settings must be set at the appropriate baud rate and communications settings The baud rate is user definable on the instrument the communication settings are 8 data bits no parity 1 stop bit flow control Xon Xoff Any standard computer communications program may be used to interface Windows 3 X Windows 95 and Windows 98 all have standard communications programs Windows 95 and 98 use HyperTerminal To access this find the Accessories folders and open the file Hypertrm exe The set up will walk the user through communication parameters Be certain to select the appropriate COM port for your computer when prompted for Connect Using The RS232 ports may be configured to output any reading data in any order desired Extensive discussion of the operation of the serial ports is beyond the scope of this manual Contact Reichert refractometer technical support if you require more detailed instructions 8 0Servicing Other than routine maintenance and calibration the ARIAS should only be serviced or repaired by Reichert factory trained service and repair technicians If service is required please contact the Reichert 6 Service Departme
14. ace asample on the surface ofthe lower Measuring Prism Lower the upper Illumination Prism onto the sample a magnetic latch holds the upper prism in place aligned with the lower Measuring Prism Look through the eyepiece rotate the Shadowline Adjustment Knob until the shadowline is centered in the crosshairs adjusting the focusable eyepiece if necessary for vision correction Press the READ button to obtain the sample reading Note Ifthe AUTOMATIC READING METHODis enabled simply rotate the Shadowline Adjustment Knob until the shadowline intersect falls within the crosshairs of the eyepiece Pressing READ will automatically calculate the sample reading as ifthe shadowline were centered on the crosshairs To select which reading method you wish to use read 4 3 MENU Options Reading Method Calibration reading method mode and other parameters may be modified by pressing the MENU key and altering the setting for the individual factors desired The following section details each parameter Reading Result Mode Channel and ID may be modified directly from the Reading screen 4 2 Changing Reading Mode ID and Channel From the Reading screen To change the Reading Mode i e Index Index TC Solids Solids TC press the SELECT key until an UP anda DOWN arrow appear in front of the Mode Pressing the UP or DOWN arrow onthe KEYPAD will allow the user to change the reading mode without having to access the MENU ToChangethe ID press the S
15. cant source of variance in traditional manual refractometers especially in facilities where multiple users are analyzing samples having unclear shadowline boundaries The sharpness of a shadowline boundary is dependent upon the sample as wellas the cleanliness of the instrument Non homogenous samples or samples with air bubbles may produce fuzzy indistinct shadow lines These type of samples are best analyzed using the Automatic Reading Method to eliminate operator interpretation Color dispersion also causes some operator interpretation variance in standard abbe type refractometers which use white light and color compensation prism rotation The ARIAS uses LEDs in the range of 589 nm as well as a 589 nm internal filter to eliminate color dispersion Mode The ARIAS may be usedin any of four reading modes Refractive Index Refractive Index Temperature Compensated Percent Solids and Percent Solids Temperature Compensated To select a mode simply highlight Mode and press SELECT The four avail able modes will be listed and may be selected by using the UP and DOWN arrow keys and pressing SELECT when the desired option is highlighted Note The temperature compensation and percent solids results are based upon data from the Channel which is currently being used In Channel One this information is based upon the ICUMSA data for Sucrose solutions Language English French German and Spanish languages are currently availab
16. d with the Adjust Span option Choosing Adjust Set Point will allow the user to perform a crosshair calibration of distilled water Typically recalibration with distilled water on a routine basis is allthatis necessary to maintain accuracy Choosing the Adjust Span option will allow the userto use between 1 to 6 additional calibration points to complement the Set Point calibration of water These points can be highlighted and toggled on off such as in the example in Figure 1 Inthis example 1 40229 and 1 51416 points have been selected as indicated by a nexttothe selected points and willbe used for calibration Highlightand select Continue to proceed through the step by step crosshair calibration using these two points These points willremain active untilthe user switches them off the nexttime a span adjustmentis performed The unit will now use only these points along with that of distilled water in its calculations In this example three points determine howtheunitis calibrated Otherexamples would range from a 2 pointup to a 7 point crosshair calibration depending onthe needs ofthe user Calibration with distilled water and at least one Span Calibration calibration standard such as 1 40229 the one provided is 1 45755 recommended to ensure 1 48993 accuracy across the range of 1 51416 calibration Note that atleast 1 57230 one span point MUST be active for the instrumentto operate correctly 1 63040 Exit
17. eristics of the sample A product demonstration is suggested to validate the true precision and accuracy of laboratory equipment for specific samples Calibration Oil Certified calibration oil included 1 51416 Refractive Index Value Operating Temperature Range 10 80 Sample Types Transparent translucent and semi translucent liquids solids Dimensions 11 Lx 6 25 Wx 13 75 H 280 mm x 258 mm x 345 mm Net Weight 17 25 lb 7 84kg Sample Temperature Control Water bath circulator ports on upper and lower prism Prism Assembly Stainless steel assembly with glass prism Data Output Two 9 pin RS232C serial ports Baud Rate User definable up to 57 600 bps Calibration 1 Point Water 2 7Point Water and refractive index standards or user defined standards Calibration History Record Maintains hundreds of the most recent calibration records Channels Channel 1 Channels 2 15 Brix Sucrose per ICUMSA User defined numerous scales available Automatic Temperature Equilibration Automatically detects the temperature equilibrium point between the sample and the ARIAS 500 Critical for accurate refractive index measurement Date and Time Year 2000 Compliant International format Password Setup can be password protected Languages English German Spanish and French Reichert Analytical Instruments Reichert Inc 3374 Walden Ave Depew NY 14043 USA Toll Free 888 849 8955 Phone 716 686 4500 Fax 716 68
18. l deactivate this feature The Change option will allow the user to define a new 6 digit password The Set Up option allows the user to define which features are to be password protected within the menu structure This allows the user to lock certain features while others may be modified without entering a password Follow the on screen instructions RS232 Baud Anytime a reading is taken the data is displayed on the LCD and is also sentto the two serial ports located on the left side of the instrument This option enables the user to define the Baud rate for each of these ports up to 57 600 bps Note that the Baud rate for the REAR port mustbe greater than or equal to that of the FRONT port Follow the on screen instructions to set the Baud rate The communications settings are 8 data bits no parity 1 stop bit Flow Control Xon Xoff i e software handshaking Time The time may be set in either standard AM PM format or in 24 00 00 hour military time Using the UP and DOWN arrow keys changes the value of the highlighted section while the READ key scrolls the cursor from left to right Press SELECT once the time is set appropriately Date The date may be set in the international Day Month Year format Note that the month is set and displayed with letters while the day and year are defined by numbers To set the date use the UP and DOWN un keys to modify and the READ key to scroll between day month andyear Contrast The illumination
19. le for the on screen display output Simply highlight the desired language and press SELECT Note custom channel designation is not modified by Language selection Auto Temp Equil This stands for Automatic Temperature Equilibration Detection This option will automatically detect when the sample and prism have reached a thermal equilibrium state This option may be set OFF or from 0 10C to 0 010C The value corresponds to a temperature stability Temperature stability is defined by the amount of temperature change within a 30 second period of time Experiment with specific samples to determine the ideal setting Typically a setting of 3 will be adequate When this option is activated a chart will appear whenever a reading is initiated This chart depicts the temperature y axis versus time x axis in degrees celsius versus seconds Pressing SELECT when this chartis active willimmediately initiate a reading temporarily disabling this feature for that particular reading Password The MENU maybe protected from accidental tampering by enabling this option Once enabled the user willbe prompted to enter a6 digit password When entered the MENU may only be accessed by entering this password The password also disables the ability to update the software version through the serial port via flash programming The options listed under password are On Off Change and Set Up Selecting On will activate the password function Off wil
20. lowered by gradually increasing or decreasing the temperature of the circulating fluid To connect a water bath circulator to the ARIAS attach the output hose from the water bath to either of the water bath ports on the lower Measuring Prism assembly Attach a second piece of tubing to the other lower Measuring Prism assembly water bath port and connectit to one of the water bath ports on the upper Illumination Prism assembly Ensure that this tubing is long enough to allow the upper Illumination Prism assembly to open and close freely Next attach the return hose for the water bath circulator to the remaining water bath port on the upper Illumination Prism assembly Note Water bath tubing having an Inside Diameter of 25 inches 6 4mm shouldbe used to connectio the water bathports onthe ARIAS The tubing should flex easily Overly ngid tubing may put torce on the prism preventing itfromclosing completely thus affecting reading accuracy 5 Maximum Head for water bath attachment should be 5 feet maximum pressure 15 psi Minimum operating temperature of ARIAS is 10 C maximumis 80 C ALWAYS USE THE TEMPERATURE READING FROMTHE ARIAS WHEN DETERMINING TEMPERATURE FOR READINGSORINSTRUMENTOPERATING TEMPERATURE 6 1 Helpful Hints for Water Bath Circulator Use Due to the nature of refractometer thermal efficiency controlling the temperature ofthe instrumentto a temperature above ambient operating temperature will require the water bath circulat
21. nformation regarding custom channel programming contact your Reichert representative 2 Refractive Index Temperature Compensated values are based upon the Sucrose conversion factors provided by ICUMSA For product specific temperature compensation itis necessary to develop a custom channel relating the refractive index at various temperatures for each product For instructions to program a custom channel please contact Reichert Microsystems at 716 686 3000 USA and ask for Customer Service Analytical Instruments or speak with your local Reichert representative The ARIAS 500 uses six LED light sources in the range of 589 nanometers wavelength for sample illumination The instrument also features a 589 nanometer filter internally as a redundant system to eliminate stray light contamination This industry standard wavelength has been specified by ICUMSA for sugar analysis and is applicable for most other solutions For information regarding other available wavelengths please contact a Reichert representative 20 Unpacking Electrical Considerat Environmental Conditions CAUTION As a safety precaution the power cord has been grounded to the instrument base Never use a 2 prong adapter between the power cord and the power source it will render the grounding feature ineffective CAUTION Use of this equipment other than in the manner specified in this manual may result in reduction of safety protection Carefully remove the inst
22. ntat our Reichert Analytical Division headquarters in Buffalo New York USAat 716 686 4500 Country Code 001 Outside the United States please contact your local Reichert representatives 90 Refractometer Use Techniques and Refraction Principles Refractive Index measurements and thus Percent Solids results derived from them are dependent upon temperature For mostsamples raising the temperature of a sample will lower the refractive index of thatsample Lowering the temperature will raise the refractive index This change in refractive index due to a change in temperature will cause an apparent change in concentration unless the reading is Temperature Compensated The Percent Solids TC scaleinthe ARIAS is temperature compensated based upon sucrose conversion factors developed by ICUMSA Non sucrose solutions will have unique temperature compensation factors dependent upon chemical composition concentration and temperature range Contacta Reichert representative to learn more about temperature compensation for non sucrose solutions 9 1 Sample Analysis Hints Shadowline clarity is essential to obtaining the maximum accuracy from the instrument particularly in the Manual Reading Method Here are some helpful hints for improving sample analysis Measuring viscous samples such as high sugar content syrups may be more difficult than measuring low viscosity samples Stir the sample on the Measuring Prism surface with a rubber spatula or
23. ortemperature to be set higher than the desired reading temperature on the ARIAS Conversely itwill also be necessary to setthe water bath circulator at a temperature lower than the desired operating temperature to control the instrument to atemperature below ambient The exact temperature setting is dependent upon circulating fluid type flow rate ambient temperature humidity etc Experimentation may be necessary to determine an appropriate water bath circulator setting to maintain a stable temperature onthe ARIAS When attempting to determine the appropriate water bath temperature setting it may be helpful to have a sample on the prism to emulate reading conditions Water may be used to simulate a sample if necessary The sample will act to improve the heat transfer between the upper and lower prism assemblies Maintaining water on the prism when notin use will also help to maintain a steady thermal state of the instrument and will thus help reduce sample equilibration time 7 0 Computer Interface The ARIAS may be interfaced with an IBM PC compatible computer through either of the RS232 ports located on the left hand side of the instrument Data may be downloaded to the computer and custom channel files may be sent from the computer to the refractometer through these ports In addition the ports may be setup in transparent mode This means that data sent to the instrument in one port will be transmitted out the other To interf
24. r It is intended for use on transparent translucent and semi translucentliquid samples Itmay also be used for solid samples such as glass and polymers in the manual reading method only The accuracy of readings is largely dependent upon operator skill sample condition and operating parameters As with any analytical instrument quality control standard verification should be performed routinely calibration should be performed as needed The ARIAS 500 is designed for years of accurate reliable trouble free operation provided that good laboratory practices are followed Any residue left on the prism will affect the apparent accuracy of the instrument For cleaning tips please refer to SECTION 4 4 Care and Cleaning Preparing the Prism Surface for Samples The instrument is programmed to read out directly the Percent Solids Refractive Index and Temperature of samples The Percent Solids scale is based upon the ICUMSA conversion tables relating refractive index to percent solids concentration for sucrose solutions Non sucrose solutions may be read directly in the Refractive Index mode non temperature corrected or in the Percent Solids mode provided that a conversion factor is known Custom channels may be programmed into the unit relating product concentration to refractive index and temperature Custom channels allow the instrument to read out directly in product specific concentration and temperature compensated values For more i
25. rument from the factory shipping material Check the instrument and shipping containers for any evidence of shipment damage Retain all shipping material for future use Assure thatall materials have been included with the instrument Among the items included with the instrument will be Part Number 13K41330 Certified Calibration Oil 1 51416 Refractive Index Value approximate e Part Number 1310450 140 Dust Cover Power Cord The instrument is equipped with an autosensing switching AC power supply Voltage Supply Range 100 to 240 Volts AC 50 60 Hz Input Power 40 W PMAX Environmental Conditions Indoor Use Only e Atmospheric Temperature 10 C to 45 C 50 F to 113 F e Maximum Relative Humidity 80 for Temperatures up to 31 C Decreasing Linearly to 50 Relative Humidity at 40 C Installation Category II Overvoltage Category e Pollution Degree 2 Asan optical instrument refractometers are subject to several environ mental factors which may affect performance including but not limited to vibration electrical supply interruptions power spikes etc Please ensure that the instrumentis located in an area of minimal vibration with aconstant power supply having ground fault and surge protection The instrument is equipped with two line fuses located adjacent to the power cord receptacle Replace with 400mA time lag T or Blue Use only the fuse type and size specified 3 0 Instrument Components Set
26. the instrumentso thatthe Eyepiece Display and Reichert Logo are forward and the Measuring Prism Illumination Prism and LED Light Source are in the rear The Shadowline Adjustment Knob will be on the right hand side The power cord receptacle is located on the underside ofthe baseplate The On Off switch will be located on the right hand side of the instrument below and in front of the Shadowline Adjustment Knob The two RS232C Serial Ports will be located on the left hand side The Liquid Crystal Display is surrounded by five touch pad buttons On top are the MENU and READ key below are the UP and DOWN arrows and the SELECT key Functionality of these keys is explained further in SECTION 4 0 Operating Instructions The eyepiece focus may be adjusted by rotating the eyepiece clockwise or counter clockwise until the crosshairs appear in focus when looking into the instrument through the eyepiece To adjust the focus place a sample of water on the lower Measuring Prism surface close the upper Illumination Prism in place onto the lower Measuring Prism Looking through the eyepiece adjust the Shadowline Adjustment Knob until the shadowline intersection of the light and dark fields comes into view Rotate the eyepiece until the crosshairs and shadowline appear in focus Dry both prisms carefully 4 0 Operating Instructions 4 1 Start Up and Basic Sample Analysis Plug the instrumentinto an acceptable power source Turn the instrument on Pl
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