Series 200 Oven Family
Contents
1. high See Table 1 2 for all the oven specifications The case is 34 0 cm 13 4 in wide but additional space is required on each side for the tubing to the column oven The case is 48 0 cm 18 9 in deep but additional space is required behind the unit to accommodate the power cord which is at the rear of the column oven see Figure 2 2 Solvents Mobile Phase Use only HPLC grade solvents in all analyses HPLC grade water and methanol 1 liter each are required for performance verification Installing the Oven Unpack Your Series 200 Peltier Column Oven 2 3 Unpack Your Series 200 Peltier Column Oven Carefully unpack the Series 200 Peltier Column Oven Then check for obvious signs of damage that may have occurred during shipment Immediately report any damage or missing items to the shipping carrier and PerkinElmer A Start Up Kit is supplied with the oven Use Table 2 1 to check the Start Up Kit parts Table 2 1 Start Up Kit for the Series 200 Peltier Column Oven Items Included P N Quantity AC Line Cord 0998 8986 1 Fingertight Nuts 0992 0513 2 Inlet Union 0990 3289 1 2 Amp Fuses 0999 1637 2 4 Amp Fuses 0999 1689 2 Instruction Manual 0993 6398 1 Customers located outside the U S must order a line cord that is appropriate for their location Electrical Requirements and Settings The Series 200 Peltier Column Oven can operate at 50 or 60 Hz and requires a grounded outlet that provides2. precisely Page 1 of 6 Series 200 Accessory Control Installation Guide 1 Requirements 1 1 Software Requirements e PerkinElmer Series 200 Accessory Control software e PerkinElmer Turbochrom 6 1 2 or TotalChrom 6 2 Workstation WS or Client Server CS software 1 2 Hardware Requirements e PerkinElmer Series 200 Peltier Column Oven Part number N260 1580 or Series 200 Accessory Controller e Rheodyne LabPRO 6 Column Selector Rheodyne Part numbers PR501 104 01 Stainless Steel or PR551 104 01 PEEK previously purchased as Special through PerkinElmer 1 3 Terminal Server Connection If a Terminal Server is used to connect the modules the following hardware is required e 8 port Lantronix Terminal Server Part number S200 0062 Note The older terminal servers require a Transceiver Part number S200 0009 e Corresponding adapter s supplied in Series 200 Accessory Control Kit e An RJ12 cable for each module Part numbers 7ft 2m S501 0037 25ft 7m 501 0038 50ft 15m S501 0039 100ft 30m S501 0040 e An RJ45 cable to connect the Terminal Server to the network Contact Lantronix for available cables 1 4 Serial Port Connection If the modules are connected via the computer s serial ports the following hardware is required e O pin Com serial port s must be available on the computer A RS 232 modem cable 25 pin male to 9 pin female for each oven supplied with each column oven e ANull modem 25 pin RS
3. 1416 Figure 2 3 AC Outlet configurations Installing the Oven Connect the Tubing 2 7 Connect the Tubing 1 Use standard 1 16 in stainless steel or titanium tubing and standard 1 16 in nuts and ferrules for all tubing connections you make to the column oven inlet and to the column see Figure 2 4 Note PEEK tubing with fingertight connections can also be used 2 Connect your 1 8 in drain tubing to the drain on the left side of the column chamber and place the other end in the waste container Turn on the Oven After installing the Peltier Column Oven read Chapter 3 Using the Column Oven before turning it on Turn on the Oven 2 8 Installing the Oven From Pu To Colu gt Bottom View of th Series 200 Au Series 200 Peltier Colu als ENT 31 0 a FR Solvent Series 200 Diode Array Detector In Line Solvent Fiter Optional Detai Lower Front Panel Removed Hat E x hang er IN Hea amp da an ge O UT ToD ed or Figure 2 4 Series 200 Peltier Column Oven in a typical LC system Using the Column Oven 3 Operating OVINA aaa aaa 3 1 Operating SUMMAT i eerie er ie ilo ian iai
4. Performance Verification IPV To maintain functional performance PerkinElmer recommends a yearly Instrument Performance Verification IPV of the Series 200 Peltier Column Oven by a PerkinElmer Service Engineer to ensure its operation within published specifications These tests consist of measuring the most important Peltier Column Oven characteristics such as retention time repeatability Certification is available for regulatory compliance Contact your local Perkin Elmer Sales and Service office Routine Inspection and Suitability Test To ensure that the LC system is operating within established criteria for example peak resolution peak asymmetry precision retention time column plate count pressure limits signal noise ratio etc perform a system suitability test which closely resembles the intended assay prior to running a sample analysis with the Series 200 Peltier Column Oven in the LC system While the Universal Test Mix UTM P N 0089 0893 can be used for a system check we recommend that you develop a separate system suitability test and acceptance criteria for each of your assays S 8 Safety and Regulatory Information Hazardous Chemicals Hazardous Chemicals Before using mobile phase solvents you should be thoroughly familiar with all hazards and safe handling practices Observe the solvent manufacturer s recommendations for use storage and disposal These recommendations are normally provided in the material s
5. left front panel of the oven see Figure 1 2 Connect one end of a length of 1 8 in tubing and place the other end in a waste vessel see Figure 2 4 1 6 Introduction Introducing the Series 200 Peltier Column Oven On the Back Panel On Off Switch Fuses RS 232 AC Input Figure 1 4 Back panel of the Peltier Column Oven Power On Off switch A rocker type switch located above the power cord connector It supplies AC power to the Peltier Column Oven Fuse holder Located between the on off switch and power cord receptacle it holds the system fuses The Peltier Column Oven is shipped with two 2 amp fuses installed for 240 Volt operation Note Two 4 0 Amp slow blow type T fuses are provided for 120 V operation Two 2 0 Amp 240 Vac Type T 5 x 20 mm fuses are supplied for those who wish to operate the instrument with 240 V See Chapter 2 Installing the Oven for the procedure for installing the fuses and or changing the line voltage Power cord connector AC input Accepts the detachable three prong power cord Voltage selector switch Allows choosing the appropriate line voltage RS 232 Connector Allows control of the Peltier Column Oven by Turbochrom rev 6 2 Introduction Warranty Exclusions and Limitations 1 7 Warranty Exclusions and Limitations The Limited Warranty set forth on PerkinElmer s ORDERING INFORMATION form applies to the Series 200 Peltier Column Oven Among those items excluded from the warra
6. of PerkinElmer Inc Contents Safety and Regulatory Information Electromagnetic Compatibility EMC seeren S 1 United TS EGERS sasso darla ine S 1 No 0 Ks cx odo redi ade ali ieri S 1 Conventions used in this Manual nenn S 2 Instrument Symbols and Warnings S 2 SYMDO fee T alal S 2 Warnings on the OVOt uocem ore eo depen ope desea les S 4 Electrical Warnings aod ome ae S 6 Quality Control Good Laboratory Practices nennen S 7 Quality CONO ORRORE ARR dis se olen rea 2 S 7 Certificate of System Control S 7 Instrument Performance Verification IPV S 7 Routine Inspection and Suitability Test S 7 Hazardous ChemicalS 20 Ba S 8 Definitions in Warning for Hazardous Chemicals S 8 Temperature Humidity and Environment eee S 9 Storage Conditions oo inmunda eo atio hie bbo d Paste es S 9 Introduction About the Manual erret is 1 1 Chapter SONNE ds 1 1 Introducing the Series 200 Peltier Column Oven 1 2 About the Oven Controls Indicators and Connections 1 5 Warranty Exclusions and LimitationS i 1 7 Maintenance re DER trente tte te 1 7 Warnings and Precautions i 1 7 SO VES ade elu ele cata 1 7 Buffersz release 1 8 e saure 1 8 AIF BUDDIGS eno elena 1 8 Pressure Builds es nn nn Een etre 1 9 Spedfi cation O TUR 1 10 Contents Installing the Oven Prepare Y our Laboratory 2 1 Require
7. pump detector autosampler etc see Figure 2 1 Also when using this stacked configuration band broadening is reduced by keeping the tubing from the pump to the column and or from the column to the detector to a minimum Figure 1 2 Front view of the column chamber showing the column inlet and outlet fittings The oven is controlled via a general purpose temperature programmer see Figure 1 3 The keys and LED displays on the programmer controller are discussed below e Top Green display continuously shows the current oven temperature in C e Bottom Red display shows the stored oven temperature setpoint in C e vj and A keys lower and raise respectively the column oven temperature Introduction Introducing the Series 200 Peltier Column Oven 1 5 key for Service use only key resets solvent leak alarm Figure 1 3 Peltier Column Oven controller About the Oven Controls Indicators and Connections On the Front Panel e Oven Controller see Figures 1 1 and 1 3 Used to increase and decrease the oven temperature e VW and A keys Used to lower and raise the oven temperature key For service use only e Alarm Reset Button reset solvent leak alarm e Column Inlet Outlet Fittings see Figures 1 2 and 2 4 Standard fittings which accept 1 16 in tubing for connection to the column inlet and detector e Column Drain Directs leaks away from the column chamber to a fitting under the
8. 120 Line voltage is set at the factory to 220 Vac See Installing the AC Line Cord in Chapter 2 Fuse Rating Characteristies __ Operating Conditions O Storage Conditions SSS Dimensions Height 16 0 cm 6 3 in Width 34 0 cm 13 4 in Depth 48 0 cm 18 9 in Ke 019 1 12 Introduction Specifications Installing the Oven Prepare Your Laboratory tn uan senile dalia 2 1 Required Ait Qualily arenile alia aria ern 2 1 Sufficient Electrical Power rivi 2 2 Space Requitemehts suna ala e 2 2 Solvents Mobile Phase nai a id 2 2 Unpack Your Series 200 Peltier Column Oven i 2 3 Electrical Requirements and Settings 2 3 Oven Power Cord and Fuses ss 2 4 AC Tse Eord ET EE EN etae e Certe ip tae Te an 2 6 Connect the Tubing nana ite verto ere es 2 7 TINO lil rn LIL AIN 2 7 Installing the Oven This chapter describes how to install your Series 200 Peltier Column Oven The topics covered in this chapter are e Prepare your laboratory e Unpack your oven e Electrical requirements and settings e Connect the tubing e Turn on the oven Prepare Your Laboratory Before installing your Series 200 Peltier Column Oven prepare your laboratory according to the following guidelines Required Air Quality To minimize contamination problems provide a relatively dust free environment Make sure that the kinds of gases or vapors listed are not present at level
9. 232 cable Rheodyne part number 5123 067 for each column selector purchased as Special through PerkinElmer e For column selector Standard 9 pin computer serial ports require 25 pin male to 9 pin female converter from any computer store pP PerkinElmer precisely Page 2of6 The following should be completed in conjunction with the Series 200 Accessory Control Installation Checklist 2 Hardware Installation 2 1 Column Oven Install the column oven including power cable see Installation section in the PerkinElmer Instruments Series 200 Peltier Column Oven or Series 200 Accessory Controller User s Guide If the column oven is connected via a serial port on the local computer install the RS 232 modem cable supplied with the oven connecting the oven to the computer If a Terminal Server is used to connect the column oven install the corresponding adapters and RJ12 cables to connect the module to the Terminal Server see below 2 2 Column Selector Install the column selector including power cable Not necessary if part of Series 200 Accessory Controller proceed to next paragraph Important Note Proceed in the following order first power on the column selector and then connect the RS 232 Terminal Server cable This is especially important if the other end is already connected to active computer port Terminal Server On the Series 200 Accessory Controller the cable connection for the column selec
10. Config and select the Job Types tab Select Job Type to be changed For TC administrators this is typically manager Select the Navigator Apps Menu button Select the New Document icon and enter the following menu text Series 200 Accessory Control Hands On Next using the Folder icon select Hands On exe from the directory C Program Files PerkinElmer Series 200 Accessory Control or wherever installed via step 7 in section 3 1 above On the Job Types tab select the User Program Access button Verify that this job type has access to all user programs 1 option pP PerkinElmer precisely Page 6 of 6 4 2 For user accounts 1 2 3 4 5 6 7 Now select the Job Types for the other users of the software Note Steps 2 7 must be performed for all job types representing users intending to use Series 200 Accessory Control Hands On Select the Navigator Apps Menu button Select the New Document icon and enter the following menu text Series 200 Accessory Control Hands On Next as in step 7 of section 3 1 using the Folder icon select Hands On exe On the Job Types tab select the User Program Access button Select this job type to have access to the following program PCcontrol exe for TotalChrom enter the following Description Automated Series 200 Accessory Control Program Select File gt Save and Reconfigure to save the co
11. LIQUID CHROMATOGRAPHY SERIES 200 OVEN FAMILY User s Manual gt PerkinElmer precisely Series 200 Oven Family User s Manual Release History Publication Date 09936398 D October 2003 Any comments about the documentation for this product should be addressed to User Assistance PerkinElmer 710 Bridgeport Avenue Shelton Connecticut 06484 4794 U S A Or emailed to info perkinelmer com Notices The information contained in this document is subject to change without notice Except as specifically set forth in its terms and conditions of sale PerkinElmer makes no warranty of any kind with regard to this document including but not limited to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior written permission of PerkinElmer Inc Copyright 2003 PerkinElmer Inc Produced in the USA Trademarks Registered names trademarks etc used in this document even when not specifically marked as such are protected by law PerkinElmer is a registered trademark
12. a nominal 120 or 240 Vac source If the electrical supply voltage in your laboratory produces large AC line voltage fluctuations install a voltage regulator between the AC line voltage outlet and your LC system Instrument grounding is required and accomplished by a third wire in the AC line voltage outlet box If your electrical outlet does not contain a ground contact your local electric company 2 4 Installing the Oven Electrical Requirements and Settings Oven Power Cord and Fuses The AC input power module is located on the left side of the back panel and contains the on off switch and system fuses On Off Switch Fuses RS 232 AC Input Figure 2 1 Rear panel of the oven The unit is shipped with two 2 0 amp fuses installed for 240 Volt operation Prior to operation at 120 VAC set the operating voltage and replace the fuses as described below To replace a blown fuse proceed as follows see Figure 2 1 1 Turnoffthe AC power to the oven by pressing 0 on the On Off switch on the rear of the oven Then unplug the AC line cord from the AC outlet 2 Toremovea fuse use a screwdriver to pry the fuse holder from the power input block 3 Install the appropriate fuse e 2amp fuse P N 0999 1637 for 240 volt operation e 4amp fuse P N 0999 1689 for 120 volt operation 4 Make certain that the Voltage Selector block is in the correct voltage position Installing the Oven Electrical Requirements and Setting
13. a rire 3 2 Shutting Down the System 3 3 Using the Column Oven In this chapter we explain how to routinely operate the Series 200 Peltier Column Oven Before operating the oven carefully read Chapter 2 Installation and make a final check of the column connections to and from the oven We have included a simple paragraph before the operating procedure that explains how the oven works After the operating instructions we tell you how to shut down the LC system overnight or for weekends Operating Overview The Series 200 LC column oven includes e Front access column chamber e Open bed design which accepts different column sizes e Solvent leak sensor e Temperature control via a general purpose temperature programmer The mobile phase and sample from the Series 200 Pump enter the column inlet fitting at the right of the column oven see Figure 2 1 then pass through a heat exchanger to bring the mobile phase to the column oven set temperature The specifications of the Series 200 Peltier column oven and Column Oven are included in Tables 1 2 and 1 3 3 2 Using the Column Oven Operating Summary Operating Summary Make sure that the oven AC line cord is connected to the proper line voltage See the paragraphs Electrical Requirements and Setting the Operating Voltage in Chapter 2 After switching on the oven power proceed as follows 1 Setthe oven temperature by pressing the W and A keys 2 Redis the set temperatu
14. afety data sheets MSDS supplied with the solvents become hazardous after completion of an analysis The responsible body for example the Lab Manager must take the necessary precautions to ensure that the surrounding workplace and that the instrument operators are not exposed to hazardous levels of toxic substances chemical or biological as defined in the applicable Material Safety Data Sheets MSDS or OSHA ACGIH or COSHH documents Venting for fumes and disposal of waste must be in accordance with all national state and local health and safety regulations and laws Warning Some chemicals used with this instrument may be hazardous or may Definitions in Warning for Hazardous Chemicals Responsible body Individual or group responsible for the use and maintenance of equipment and for ensuring that operators are adequately trained per IEC 1010 1 Amendment 2 Operator Person operating equipment for its intended purpose per IEC 1010 1 Amendment 2 OSHA Occupational Safety and Health Administration United States ACGIH American Conference of Governmental Industrial Hygienists COSHH Control of Substances Hazardous to Health United Kingdom Safety and Regulatory Information Temperature Humidity and Environment S 9 Temperature Humidity and Environment Caution This instrument is designed for indoor use only Warning This instrument is not designed for operation in an explosive environment Safe Op
15. anual This manual contains important information regarding potential hazards that may arise during the operation of the Peltier column oven All references except where indicated in this manual that refer to the Series 200 Peltier Column Oven also apply to the Series 200 Column Oven It is essential that the Safety and Regulatory Information preceding this chapter be read and thoroughly understood by all potential users of the oven instructions provided in the manual If used otherwise the A Caution The Peltier column oven should be used according to the protection provided by the Peltier column oven may be impaired Chapter Content In the first chapter of this manual we describe the Series 200 Peltier column oven and its controls In the second chapter we describe installation of the oven in your LC system Electrical requirements and tubing connections are also included in Chapter 2 In the third chapter we include principles of operation for the oven routine operating procedures and the procedure for shutting down the system This manual is written for a person who has developed a working knowledge of liquid chromatography and understands e The function of each major component in a liquid chromatography system e How to select a column based on the analysis you wish to perform e The principles of solvent selection and sample preparation 1 2 Introduction Introducing the Series 200 Peltier Column Oven If you require an intro
16. d Air Quality sci soleil e ts 2 1 Sufficient Electrical Power vesicle Edda 2 2 Space REGUITEMENES 3 sro stanze tbt eode ta nea ptio tesi 2 2 Solvents Mobile PTISsa uia teret ac risi 2 2 Unpack Your Series 200 Peltier Column Over 2 3 Electrical Requirements and Settings 2 3 Oven Power Cord and FUSES nennen 2 4 AC Line COP nocere nissan nn 2 5 Connect the Tubind o deco ete a tote oon 2 6 TUR OH Ee OVA 2 7 Using the Column Oven Operating Overview 3 1 Operating SUMMArY ivi eerie en 3 2 Shutting Down the Syste s sues en 3 2 Appendix Series 200 Accessory Control Installation Guide Safety and Regulatory Information Electromagnetic Compatibility EMC United States This product is classified as a digital device used exclusively as industrial commercial or medical test equipment It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15 103 c Addresses To obtain assistance with the Series 200 Peltier Column Oven contact the Company as described below User Assistance PerkinElmer 710 Bridgeport Avenue Shelton Connecticut 06484 4794 Tel 800 762 4000 Fax 203 762 6000 Supplies accessories and replacement parts can be ordered directly from PerkinElmer using the eight digit part numbers provided in the manual To place an order or ask for information call your local PerkinElmer representative S 2 Safety and Regulatory Inf
17. duction to these concepts the following book is an excellent reference Practical Liquid Chromatography An Introduction by R W Yost L S Ettre and R D Conlon published by The Perkin Elmer Corporation 1980 P N 0993 9656 Introducing the Series 200 Peltier Column Oven The Series 200 Peltier Column Oven controls the column temperature variations in column temperature can cause significant changes in retention times The oven provides controlled temperatures which aid analyses by decreasing mobile phase viscosity and increasing mass transfer and sample solubility The oven also provides cooling i e the capability of controlling the oven temperature in the 10 to 25 C region The major features of the Peltier Column Oven include the following e A low dispersion heat exchanger which heats the mobile phase and sample before it enters the column The injected sample remains a plug as it passes through the heat exchanger which prohibits peak broadening Because of this solvent preheating technique the mobile phase encounters a stationary phase of uniform temperature over the entire length of the column e An open bed design which eliminates the need for specific blocks or inserts for different column sizes Up to three 3 8 in x 30 cm or three Y in x 30 cm HPLC columns can be easily placed inside the column oven The column oven chamber is large enough to accommodate a guard column and an analytical column for precise temperature contr
18. dware the HPLC system and the printer are turned on pP PerkinElmer precisely Page 4of6 3 Software Installation 3 1 Server Application Server and Client Application 1 Itis recommended that one install the server application on the same computer on which the Turbochrom TotalChrom TC Server is installed In the Series 200 Accessory Control Configuration Checklist enter the name of the server on which Series 200 Accessory Control server application will be installed The Configuration Checklist is located on the installation CD or in section III of the Appendix within the Series 200 Accessory Control QOQ Note On a Client Server system install the server application once on only one computer 2 To install the Series 200 Accessory Control software log on as a Windows administrator 3 Run the Setup EXE on the installation CD 4 Follow the instructions on the Welcome screen 5 On the Installation Type screen select to install the Server and Client Applications Note To install the Server component Windows NT 4 Service Pack 5 or higher is required 6 On the Server Account screen enter the Domain Account This should refer to the Turbochrom TotalChrom TCProcess account which must have administrative rights Enter the account s password and password confirmation 7 On the Destination Location screen the following default directory is selected C Program Files PerkinElmer Series 200 Acces
19. eration This instrument will operate safely between 5 C and 40 C 40 F and 104 F with an ambient relative humidity of between 20 and 80 noncondensing Installation Category This instrument is able to withstand transient overvoltage according to Installation Category II as defined in IEC 1010 1 Pollution Degree This equipment will operate safely in environments that contain nonconductive foreign matter up to Pollution Degree 2 in IEC 1010 1 Storage Conditions The Series 200 Peltier Column Oven may be stored under the following conditions e Ambient temperature is 20 C to 60 C 68 F to 140 F e Ambient relative humidity is 20 to 80 noncondensing e Altitude is in the range 0 to 12 000 m Introduction About the Manual 3 da rear stl Cou boas pb e ed tee e de 1 1 Chapter Content reise tionem db dined anti aidan eaten 1 1 Introducing the Series 200 Peltier Column Oven sese 1 2 About the Oven Controls Indicators and Connections 1 5 Warranty Exclusions and Limitations ii 1 7 Malta PEE ana et 1 7 Warnings and Precautions eee ss 1 7 solvehts en e ERU tee cd 1 7 B ffersz A taa ie emite n etit eie nea 1 8 COITOSION 2 essi o codem iem Ile edem m eei d 1 8 PussibblIc m PH c r 1 8 Pressure Bulldup ete nter ar kennen ve SET ee on 1 8 Specifications poll Mlotioces 1 10 Introduction About the M
20. ge C to 90 C 39 F to 194 F Recommended Flow Range Up to 5 mL minute Temperature Accuracy 1 C between 5 C and 90 C 1 C between 10 C and 90 C 2 C between 15 C and 90 C edi 30 minutes to 60 C 140 F at 2 mL min Up to 5 C min 9 F min cooling and heating Power Requirements COC C C CNSCOC S Line Voltage Operating Range 110 120 220 240 Vac 50 60 Hz 10 Line voltage is set at the factory to 230 Vac See Installing the AC Line Cord in Chapter 2 Fuse Rating Characteristies __ Operating Conditions _ Storage Conditions SSS Dimensions Height 16 0 cm 6 3 in Width 34 0 cm 13 4 in Depth 48 0 cm 18 9 in kg Col With ambient temperatures of between 15 C and 20 C With ambient temperatures of between 20 C and 25 C With ambient temperatures of between 25 C and 30 C Introduction Specifications 1 11 Table 1 3 Series 200 Column Oven Specifications Specification Description Column Capacity Up to three 3 8 in x 30 cm columns or three Y4 in x 30 cm columns Operating Range 30 C to 90 C 87 F to 194 F Recommended Flow Range Up to 5 mL minute Temperature Accuracy 1 C between 30 C and 90 C 1 8 F between 86 F and 194 F Up to 5 C min 9 F min heating 30 minutes at 60 C 140 F for READY at 2 mL min Power Requirements fe a Line Voltage Operating Range 110 120 220 240 Vac 50 60 Hz
21. i ce r parab kparl utiisateur l int rieur Contac tez le personnel qualifi de P erkin Eimer AVERTI SSEMENT Lacontinuit du circuitde mise laterreestessentiele pour lebon fo nction nemert de l appareil Nutilisez jamais l appare illors que le cable de mise laterreest d branc h Figure S 2 Location of the rear panel warning labels on the Series 200 Peltier Column Oven S 6 Safety and Regulatory Information Electrical Warnings The following information is printed on a warning label that covers the fuse receptacle on the rear panel of the oven N Caution The Series 200 Peltier Column Oven is shipped from the factory with the operating voltage set to 240 VAC the main AC power switch set to the OFF position 2 0 amp fuses installed If your line voltage is 110 120 volts you need to switch the voltage selector setting to 110 or 120 and install the 4 0 amp fuses before installing the line cord and turning on the AC power Electrical Warnings A gt Electrical Hazard Connect the instrument to an AC line power outlet that has a protective ground connection To ensure satisfactory and safe operation of the instrument it is essential that the protective ground conductor the green yellow lead of the line power cord be connected to a true electrical ground Any interruption of the protective ground conductor inside or outside the instrument or disconnection of the protective ground terminal may im
22. nfiguration 5 Series 200 Accessory Control Configuration Note For the Series 200 Accessory Controller which incorporates both the column oven and selector into a single hardware module first configure the oven as a Series 200 Peltier Column Oven Then configure the column selector as the stainless steel SS version of the Rheodyne 6 port Column Selector If a formal IQOQ procedure is required please refer to Section III of the Series 200 Accessory Control IQOQ document After the Series 200 Accessory Control software has been installed the following steps to configure column oven selector modules and customize manager access rights have to be completed 1 2 3 From the Windows select Start gt Programs gt PerkinElmer gt Series 200 Accessory Control gt Series 200 Accessory Control Manager if this structure was customized during installation please follow customized structure Then login to Series 200 Accessory Control Configuration Manager as a manager with the default user name and password manager manager From the menu bar select Configuration gt Manager Access to open the Manager Access Configuration dialog Select the Add button to add user names and passwords for users with administrative rights in Series 200 Accessory Control Select to delete the default manager login manager Configure Communication Devices by selecting Configuration gt Communication and add c
23. nt s with the type of column s being used e Know the relative polarity and miscibility of the solvents being used Buffers Exercise care when using buffers in conjunction with organic solvents NEVER LEAVE BUFFERS IN THE SYSTEM OVERNIGHT Buffers left in the system can form salt crystals which may cause premature pump seal failure interfere with proper check valve operation and plug the connecting tubing or the detector flowcell To remove buffers flush the system first with water then with methanol or isopropanol Change the mobile phase from methanol or isopropanol to water before using buffers Corrosion All parts that contact the mobile phase are made of stainless steel Teflon PEEK or glass Some of these materials are extremely sensitive to acid chlorides If you have questions about your mobile phase contact a PerkinElmer representative Table 1 1 Solvents Which May Corrode the Series 200 Peltier Column Oven Aqua Regia 80 HCl 20 HNO Chloride salt solutions Chlorinated solvents Halogenated solvents Titanium is more resistant to chloride salt solutions Air Bubbles To prevent air from entering the system and to ensure that pressure fluctuations do not occur observe the following precautions e Ensure that the pump s solvent inlet filter is below the solvent level in the solvent reservoir e Ifthe pump has not been used for an extended period of time remove air bubbles by connecting a priming sy
24. nty under Part B EXCLUSIONS AND LIMITATIONS are e Fuses e Nuts e Ferrules The items listed above are considered to be routinely replaced as part of the normal wear of the instrument Maintenance Before using any cleaning or decontamination method except those recommended users should check with the manufacturer to make certain that the proposed method will not damage the oven Warnings and Precautions Read and understand the following warnings and precautions Solvents are using Refer to the safety data sheets provided by the manufacturer For example Material Safety Data Sheets MSDS in the USA N Warning Give careful attention to the hazards associated with the solvents you e Wear appropriate eye protection at all times when handling chemicals Use safety glasses with side shields goggles or full face shields according to the types of chemicals you will be handling e Wear suitable protective clothing including gloves that are specifically resistant to the chemicals being handled e Always use clean solvents Solvents which have been distilled in glass HPLC Grade are highly recommended e Filter the solvents through a 0 5 micron medium as an additional precaution e Carefully use and store flammable solvents which may form hazardous by products when the instrument is shut down by following the recommended shutdown procedure 1 8 Introduction Warnings and Precautions e Check compatibility of solve
25. ol e The Peltier Column Oven can be operated either manually or remotely via an RS 232C port e A leakage sensor is included in the column oven Ifa leak occurs a red light on the front panel illuminates This feature protects against potential hazards and oven damage e The anodized aluminum column chamber see the top illustration in Figure 1 1 is corrosion resistant It is light strong and enhances temperature uniformity Introduction Introducing the Series 200 Peltier Column Oven 1 3 You can install your column by simply removing the lower part of the front panel which is held onto the oven assembly only by magnets connect the fittings to the column inlet and outlet and insert the column into the holders to secure it in place amp gt Figure 1 1 Front View of the Series 200 Peltier Column Oven The top drawing shows the column chamber after removing the front panel 1 4 Introduction Introducing the Series 200 Peltier Column Oven The column chamber contains a drain line that directs solvent leaks away from the electronics Connect drain tubing to the drain fitting located under the left front panel of the Peltier Column Oven see Figure 1 2 and directs the tubing to the system waste vessel as shown in Figure 2 4 Take care not to crimp the drain tubing Since you can access the column chamber from the front the Series 200 Peltier Column Oven can be stacked with other system modules such as the Series 200
26. ommunication devices Select Configuration gt Accessory Wizard and run the Wizard to create instruments and configure column ovens and column selectors Note Only instruments that are configured in Turbochrom TotalChrom can be configured in Series 200 Accessory Control Ensure that the Instrument Key and Name match exactly SysConfig gt Instruments tab Please refer to the Series 200 Accessory Control Standard Operating Procedure Manager Configuration Guide section for a detailed description of the configuration procedure pu essentials To place an order for supplies and many replacement parts request a free e ssentials catalog or ask for information visit our website at www perkinelmer com The most up to date information on part numbers product brochures systems modules spare parts and application notes are in the PerkinElmer website Telephone U S only Call toll free 1 800 762 4000 8 30 a m to 7 p m EST Your order will be shipped usually within 24 hours e Worldwide Call your local PerkinElmer sales or service office pP PerkinElmer precisely PerkinElmer 710 Bridgeport Avenue Shelton CT 06484 4794 U S A Internet http www perkinelmer com email info perkinelmer com PerkinElmer is a registered trademark of PerkinElmer Inc Part Number 0993 6398 D
27. ormation Conventions used in this Manual Conventions used in this Manual The following graphic symbols and special text formats are used in this manual to identify important safety information Warning A warning indicates an operation that could cause personal injury if precautions are not followed Caution A caution indicates an operation that could cause instrument damage if precautions are not followed Note Notes emphasize significant information in a procedure or description Instrument Symbols and Warnings Symbols The following three types of warning symbols appear on the instrument This symbol indicates an operation or instrument condition that could cause personal injury if precautions are not followed This can be any type of hazard When you see this symbol refer to the safety pages in the manual for further information Consulter les documents d accompagnement This symbol indicates the danger of electric shock if precautions are not followed due to the presence of high voltage Attention Risque de choc lectrique This symbol indicates the danger of burns if precautions are not followed due to the presence of heat in this instrument area This may also indicate a hot surface that can be easily touched AVERTISSEMENT Surface chaude Safety and Regulatory Information Instrument Symbols and Warnings S 3 The following additional graphic symbols used on the instrument Indicates alternating curren
28. pair the protection provided by the instrument Electrical Hazard Do not operate the instrument with any covers or parts removed Electrical Hazard Do not make adjustments replacements or repairs to this instrument except as described in the User Documentation Only a PerkinElmer service representative or similarly trained and authorized person should service the instrument Electrical Hazard Use only fuses of the specified type and with the required current rating for replacement Safety and Regulatory Information Quality Control Good Laboratory Practices S 7 Quality Control Good Laboratory Practices Quality Control The user should develop appropriate quality control procedures for the LC Peltier Column Oven and the entire LC system to ensure suitability for its intended use These procedures typically consist of periodic performance verifications and routine inspections and suitability tests Certificate of System Control Each Series 200 Peltier Column Oven is carefully built and tested in a controlled system in accordance with the requirements specified in its applicable PerkinElmer Final Assembly and Test Specification Each instrument is certified to meet its functional and performance specification upon release to shipment The integrity of this quality system is routinely audited and is certified by the British Standards Institution as meeting all requirements of ISO 9001 1987 Certification No FM 22179 Instrument
29. re and green is the actual Figure 3 1 Peltier Column Oven controller Using the Column Oven Shutting Down the System 3 3 Shutting Down the System When shutting down the system overnight or for the weekend observe all precautions pertaining to hazardous solvents and or those solvents which form harmful deposits or by products 1 Remove harmful mobile phases from the LC system 2 Flush the column according to the instructions supplied with the column Flush buffer salts from the system with water evaporation leaves salt crystals which may form harmful deposits Remove chloroform or solvents which can decompose to form hydrochloric acid from the system Caution Damage caused to the system by precipitating buffer salts in capillary tubing or damage resulting from this condition is specifically excluded from warranty 3 After removing harmful mobile phases prepare the detector for most mobile phases by flushing it with isopropanol To avoid contaminating the system re filter or discard solvents including water that were exposed to the environment for more than 24 hours before use 4 For weekend storage we recommend flushing 60 40 MeOH Water through the pump column and flowcell provided your column is compatible with MeOH Water Then turn off the column oven pump and detector 3 4 Using the Column Oven Shutting Down the System Appendix Series 200 Accessory Control Installation Guide gt PerkinElmer
30. ringe to the drain valve on the pump opening the drain valve and fast flushing the system to prime the pump After priming the pump close the drain valve completely Pressure Buildup Over time you may notice a gradual increase in the system operating pressure If you observe pressure readings greater than 3 45 MPa 500 psi above the normal operating pressure of your analysis check the following items Introduction Warnings and Precautions 1 9 If you are injecting clean samples and there is no other apparent cause for the pressure buildup remove disassemble and clean the injector If you are injecting dirty samples the injector may be clogged or the column packing material may have retained contaminated particulates Remove and clean the injector and replace the column The column end fitting or column frit may be plugged Replace the end fitting or frit to relieve excess pressure Refer to the instructions supplied with the column In line filters may be plugged Replace the filter element With the pump connected directly to the detector input if you still experience excessive pressure the problem could be a plugged flowcell You can flush reverse flush or rebuild the flowcell 1 10 Introduction Specifications Specifications Table 1 2 Series 200 Peltier Column Oven Oven Selector Specifications Column Capacity Up to three 3 8 in x 30 cm columns or three Y4 in x 30 cm columns Operating Ran
31. s 2 5 To change the voltage proceed as follows see Figure 2 2 1 Remove the tan color block by pulling it from the fuse holder 2 Rotate the block and insert it back into the fuse holder so that the desired voltage displays in the small window in the fuse holder Install the appropriate fuses in the fuse holder for that voltage see Figure 2 2 4 Insert the fuse holder into the Y e VAC 2 0 A Fuses P N 0999 1637 120 VAC 4 0 A Fuses P N 0999 1689 Voltage Selector Block Figure 2 2 Removing and installing fuses 2 6 Installing the Oven AC Line Cord Electrical Requirements and Settings Ground is necessary for safe operation of the oven and proper N Warning Do not disconnect or defeat the safety ground on the power cord functioning of the electronic circuitry The line cord should have the electrical rating and proper plug for your location Figure 2 3 lists the cords with part numbers To select an AC line cord 1 Referto Figure 2 3 and select the proper line cord for your location 2 Insure that the power switch is off then plug one end of the line cord into the AC supply North America NEMA 5 15 0999 1420 Old British Standard BS 546 India 0999 1423 Europe e CEE 7 British Standard 0999 1422 BS 1363 Schuko United Kingdom 0999 1415 0999 1414 Europe Australia Switzerland ETSA S 86 0999 1413 0999 1417 Europe Israel Italy 0999 1424 Denmark 0999
32. s that exceed federal state and local ordinances for continuous human exposure e flammable e caustic e explosive e corrosive e toxic Make sure that your laboratory environment consists of the following temperature and humidity levels 2 2 Installing the Oven Prepare Your Laboratory e Ambient temperature between 10 C and 35 C 50 F and 95 F e Constant relative humidity between 20 and 80 without condensation Use care when working with hazardous solvents or solvents that produce hazardous by products performance should be considered hazardous to someone who is Warning Solvent vapor levels that are high enough to interfere with the oven continuously exposed to the vapors Sufficient Electrical Power The Series 200 Peltier Column Oven requires a grounded nominal 120 or 240 Vac source To prevent degradation of the column oven s performance the AC line voltage must remain within 10 of the nominal specified voltage displayed on the label beneath the line cord If you experience large voltage fluctuations install a voltage regulator between the LC system and the wall power outlet Space Requirements The Series 200 Peltier Column Oven is designed to sit on a bench top or on another module as shown in Figure 2 4 It is plumbed into the LC system between the autosampler and the detector A space 17 cm 6 7 in high is sufficient as columns are installed from the front of the oven The oven is 16 cm 6 3 in
33. sory Control or select the Browse button to select another destination folder 8 On the Backup Replaced Files screen it is recommended to create backups of replaced files By default the following directory is selected C Program Files PerkinElmer Series 200 Accessory Control BACKUP If in the previous dialog step 7 another destination folder has been selected the BACKUP folder will be created in the appropriate destination folder 9 On the Select Program Manager Group screen a program manager group is entered by default PerkinElmer Series 200 Accessory Control If desired enter another program manager group Note Vith the selected program manager group on NT any user has access to this program 10 On the Start Installation screen select Next to install the program 11 The program will be installed Note During installation a computer restart might be required if newer system components have to be installed After the restart the same login has to be used After the computer restart the installation process will start from the beginning Reenter the original entries 12 When the nstallation Complete screen appears the installation is finished Selecting the Finish button will complete the Installation program 13 After the installation the system has to be restarted If an immediate restart is desired select OK in the Install dialog or select Cancel to return to Windows and restar
34. t Indicates the primary protective grounding terminal Indicates the off position of the main power switch Indicates the on position of the main power switch S 4 Safety and Regulatory Information Instrument Symbols and Warnings Warnings on the Oven The following warning labels are affixed to the side and rear panels of the column oven See Figures S 1 and S 2 ATTENTION Nobstruez pas larriv e ou l vacua tion d air Caution Do notrestric tairintake orexhaust Figure S 1 Location of side panel warning labels on the Series 200 Peltier Column Oven Safety and Regulatory Information Ce Warning For protec tion a gain stfire hazard replace onlywithsametypeand ratngoffus Warning To reduce the chance of electrical sh ock don ot remove covers that requ ire tod acess Nou sers ervi eable parts areinsick Refer servicing to Perkin Elmer qualified Service persannel Warning Grounding circuit c ontin uity is vital for Sa fe operatio nof equipment Never op eate equipment with grounding conductor dis connected Instrument Symbols and Warnings S 5 AVERTISSEMENT Afin dassurer la protection contre les ris ques d incendie remplacer u nique ment par unfusi ble de m metypeet de m me courant nominal AVERTISSEMENT Pourr du ire lesrisq ues d lectr ccution ne retire pasles couve cles don t l owerture n cess ite de s out i il n y a aucune p
35. t the system later pP PerkinElmer precisely Page 5 of 6 3 2 Client Application 1 2 3 4 5 6 7 The Client application has to be installed separately on each computer on which a Series 200 Accessory Control application is desired Turbochrom TotalChrom has to be available on each of these client computers To install the Series 200 Accessory Control software log on as a Windows administrator Run the Setup EXE on the installation disk Follow the instructions on the Welcome screen On the Installation Type screen select to install the Client Application only On the Server Name screen the Server name is entered by default Note If no entry or an incorrect server name entry is present enter the name of the server on which the Server Application has been installed see Server Application Installation step 1 Complete the Client Installation by continuing with step 7 of the Server Application Installation 4 Required Turbochrom TotalChrom System Configuration Changes In order to have access to the Series 200 Accessory Control software from the Turbochrom TotalChrom Navigator s Apps menu the following changes in the System Configuration are required For every Job Type using PerkinElmer Series 200 Accessory Control software the following procedures have to be completed under Turbochrom s TotalChrom s SysConfig 4 1 For administrator accounts 1 2 3 4 5 6 7 Open Sys
36. tor is made on the back of the unit If the column selector is connected via a serial port on the local computer install the Rheodyne RS 232 null modem cable and adapter see Requirements above connecting the oven to the computer Install the inlet outlet column connections If a Terminal Server is used to connect the column selector install the corresponding adapters and RJ12 cables to connect the module to the Terminal Server see next page pP PerkinElmer precisely Page 3of6 2 3 Terminal Server Connect the Terminal Server to the network and configure the Terminal Server see Lantronix Terminal Server Installation Guide It is encouraged to check Terminal Server port parameters for Series 200 Accessory Control These parameters can be accessed by Telnetting to the terminal server and typing show ports x x being the port number to which the device is connected The parameters should be set as follows Char Size Stop Bits 8 1 Input Speed 9600 Flow Ctrl None Output Speed 9600 Parity None Modem Control None Access Remote Local Switch None Backward None Port Name Port_1 Break Ctrl Local Session Limit 4 Forward None Terminal Type Soft Preferred Services Lat Telnet Authorized Groups 0 Current Groups 0 L Characteristics oss Notify Remote Conf 2 5 General Ensure that the relevant computers the terminal server if available the column oven and column selector interfacing har
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