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1. Location Requirements The Triad Freeze Dry System should be located in an area that provides an unobstructed flow of air around the cabinet This air cools the refrigeration system The refrigeration system draws air in through the left side and exhausts it through the right side A minimum of 3 must be allowed between the rear and both sides of the Triad Freeze Dry System and adjacent wall surfaces Restriction of airflow during operation could adversely affect performance The Triad Freeze Dry System must be placed on a work surface or table that will support over 400 Ibs Refer to Appendix B Freeze Dry System Dimensions for drawings Product Service 816 333 8811 or 1 800 522 7658 5 Chapter 2 Prerequisites Vacuum Pump Requirements The user must provide a vacuum pump A vacuum pump with a displacement of 144 liters per minute and 0 0002 mBar ultimate pressure 1s adequate for most samples The inlet fitting on the vacuum pump must be suitable for 3 4 ID vacuum hose which is provided with the FreeZone Triad Freeze Dry System It is recommended that the vacuum pump is equipped with an exhaust filter to minimize oil mist exhausting from the vacuum pump The operating vacuum level may be set on the freeze dry system The higher the pressure 1s set the more likely it is that oil mist will be exhausted Vacuum pumps should be equipped with a reverse IEC plug This will allow the vacuum pump to be plugged into the receptacle on the
2. Ramping H Hold E End P Prefreeze TRM Time remaining of current hold segment in hours COL Collector 85 Collector temperature in C SHLF Shelf 55 Shelf temperature in C SAMP Sample Probe 55 Sample temperature in C VAC Vacuum 180 Vacuum in microbar 999 Function not active There are several commercially available software packages which can read RS232 data and enter the data into a computer program such as a word processor to create a text file or spreadsheet to tabulate and plot the data Consult your laboratory supply dealer regarding the latest software available The following describes how to use an IBM compatible computer with Hyper Terminal software included with Windows 95 98 XP or 2000 operating systems to collect the RS232 data from your freeze dry system 1 Make sure that the freeze dry system is properly connected to the communication port on the computer 2 Open Hyper Terminal software 1 Windows 95 or 98 use START PROGRAMS ACCESSORIES 11 Windows 2000 or XP use START PROGRAMS ACCESSORIES COMMUNICATIONS The first time Hyper Terminal is opened a dialogue box requesting an area code and phone number will appear Enter the appropriate numbers and continue Product Service 816 333 8811 or 1 800 522 7658 33 Chapter 6 Using the RS232 Receptacle 3 The Connection Description dialogue box will open Type in a user defined name and select an icon for
3. SteamScrubber amp FlaskScrubber Glassware Washers carry a two year warranty from date of installation or three years from date of shipment from Labconco whichever is sooner Blood Drawing Chairs carry a ten year warranty Carts carry a lifetime warranty Glassware is not warranted from breakage when dropped or mishandled This limited warranty covers parts and labor but not transportation and insurance charges In the event of a warranty claim contact Labconco Corporation or the dealer who sold you the product If the cause is determined to be a manufacturing fault the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation Under no circumstances shall Labconco Corporation be liable for indirect consequential or special damages of any kind This statement may be altered by a specific published amendment No individual has authorization to alter the provisions of this warranty policy or its amendments Lamps and filters are not covered by this warranty Damage due to corrosion or accidental breakage is not covered Returned or Damaged Goods Do not return goods without the prior authorization from Labconco Unauthorized returns will not be accepted If your cabinet was damaged in transit you must file a claim directly with the freight carrier Labconco Corporation and its dealers are not responsible for shipping damages The United States Interstate Commerce Commission rules requ
4. Chapter 8 Modifying Your Freeze Dry System Display If you do not want to restore all factory settings but want to adjust the vacuum offset press DISPLAY briefly and the display will show VACUUM OFFSET 0 mBar Press ff or amp until the appropriate offset is shown Each number represents an offset of 0 001 mBar Press DISPLAY and the display will show COLLECTOR TEMP OFFSET 0 C Press ff or amp until the appropriate offset is shown Each number represents an offset of 1 C Press DISPLAY and the display will show This is the probe mounted in the shelf Temperature from this probe SHELF TEMP OFFSET 0 C is displayed during Pre Freeze Press ff or amp until the appropriate offset is shown Each number represents an offset of 1 C Press DISPLAY and the display will show This is the probe mounted to the Lexsol tube LEXSOL TEMP OFFSET 0 C in the back Temperature from this probe is displayed as SHLF TEMP during sequences other than PRE FREEZE Press ff or amp until the appropriate offset is shown Each number represents an offset of 1 C Press DISPLAY and the display will show SAMPLE TEMP OFFSET 0 C Press ff or amp until the appropriate offset is shown Each number represents an offset of 1 C Press DISPLAY and the display will show PRODUCTION MENU MASTER RESET Wait approximately 10 seconds and the display will default to show operational parameters Product Service
5. unauthorized returns will not be accepted Do not discard the carton or packing material for your freeze dry system until you have checked all of the components installed and tested the system Product Service 816 333 8811 or 1 800 522 7658 7 Chapter 3 Getting Started Triad Freeze Dry System Components Locate the model of freeze dry system you received in the following table Verify that the components listed are present and undamaged If you did not receive one or more of the components listed contact Labconco Corporation immediately for further instructions Catalog Product Description 7400030 Triad Freeze Dry System 230V 50 Hz 7400040 Triad Freeze Dry System 230V 60 Hz Plus the following Part Qty Component Description 7373300 i User s Manual 1342100 1 Power Cord 230V 7373436 l Tubing 1488800 2 Clamp 1291000 l Female IEC Plug Setting Up Your Triad Freeze Dry System Component Orientation and Hose Connections A minimum of 3 should be allowed between the back of the freeze dry system and the adjacent wall surface and between the sides of the Freeze dryer and the adjacent wall surfaces Restriction of the airflow through the cabinet during operation could adversely affect performance Position the vacuum pump near the vacuum exhaust port on the rear of the cabinet Attach the supplied hose to the exhaust port using the clamp provided Attach the other end of the hose to the inlet fitting on the
6. 0 C 25 C minute from 0 C to 55 C and can heat at approximately 3 C minute Hold temperatures may be set anywhere from 50 C to 55 C in 1 C increments Hold times are set in hours Product Service 816 333 8811 or 1 800 522 7658 19 Chapter 4 Operating Instructions When the desired temperature is achieved the hold function will maintain that temperature for the programmed length of time The microprocessor control has a built in memory of the last entered program to allow the identical protocol to be repeated by simply pushing the RUN button A typical 3 segment program is shown in Figure 3 Figure 3 TIME X gt Lil a 2 Lul RAMP HOLD HOLD RAMP HOLD PR PH IH 2R 2H PREFREEZE SEGMENT SEGMENT 2 PRIMARY DRYING SECONDARY DRYING Programming The Triad Freeze Dry System will store up to five programs numbered 1 through 5 The five programs once programmed will be retained in memory To program a run select the program number that you want to use and enter the parameters as follows Turn the Power Switch to the ON position Press the DISPLAY button until the AUTO Indicator lights NOTE Values shown in this example are for reference only The P in the display will flash and the program number that will be run is shown To select different programs press the UP or DOWN button to change program number Programs through 5 When the desired program number is displayed press ENTER 20 Produc
7. 3 By pressing the up button enter a segment number that follows the current segment The current segment cannot be modified while the system is running Press ENTER 4 Modify as required 5 Press ENTER Stopping the Freeze Dry Cycle 1 At the end of the last programmed segment the freeze dry system will automatically stop The refrigeration system the heater and the vacuum pump will remain on The display will show Segment E End To shut off press RUN STOP button 2 To stop before the completion of the last programmed segment press RUN STOP The RUN STOP indicator will turn off as will the refrigeration system heater and vacuum pump The display will remain active but time functions will stop operating 3 Pressing the Power Switch will shut off the entire system 4 Ifthe power is shut off during the program when the power is turned backed on the system will resume operation if the collector temperature has not risen above 30 C The unit will then attempt to complete the programmed cycle from the point at which it was shut off If the collector temperature is greater than 30 C the system will not resume operation The RUN indicator and ALARM indicator will flash to indicate power loss during the programmed run 22 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Operating Instructions Monitoring the System Operation Press the DISPLAY button to obtain the Monitor Screen The display will show
8. 816 333 8811 or 1 800 522 7658 43 Appendix A Freeze Dry System Components The following pages list components that are available for your freeze dry system The parts shown are the most common replacement parts If other parts are required contact Product Service 7591801 Compressor 230V 50 Hz P 7591802 Compressor 230V 60 Hz 7501902 Lexsol Pump 230V 7510901 Heater 230V 4 7754800 l Pass thru Board 5 7515300 2 Temperature Sensor Lexsol Collector 6 7372500 2 Temperature Sensor Sample Shelf 7 7765501 l Diaphragm 8 7424100 l Door Gasket 9 7766801 1 Gal Heat Transfer Fluid Not Shown 10 7395300 l Printed Circuit Board Control 7448000 Printed Circuit Board RS232 re ee eee ee O a es 44 Product Service 816 333 8811 or 1 800 522 7658 pem Rs es a oO anaa i a mi abet ae 1 y 45 Appendix A Freeze Dry System Components Figure 7 Product Service 816 333 8811 or 1 800 522 7658 Appendix B Freeze Dry System Dimensions Figure 8 28 4 72cm 3 co e a 279 70 9cm Serum Bottle Capacity of the Triad Freeze Dry System Size Labconco No 100 pcs Shelf Capacity 2 ml 7575010 3 ml 7575210 5 ml 7573010 10 ml 7573210 20 ml 7573410 121 30 ml 7573610 86 50 ml 7573810 64 100 ml 7574010 42 125 ml 7574210 36 46 Product Service 816 333 8811 or 1 800 522 7658 Appe
9. Bulb Type Flat Bottom Bottom Stoppering 1 ml 7572710 7570010 757010 2 ml 7570810 7572310 4 ml 757210 5 ml 7570210 7571010 10 ml 757110 7571610 25 ml 7571810 50 ml 7572010 Trident Adapter 7762700 Three Way Adapter permits attachment of three ampules to a single valve port Ampules are attached using Ampule Valve Adapters or 1 8 surgical tubing not provided Cavity in adapter body can be filled with cotton fiber media to help prevent contamination between samples Shipping weight 3 oz 0 1 kg 54 Product Service 816 333 8811 or 1 800 522 7658 DECLARATION OF CONFORMITY Application Council Directive s 73 23 EEC 89 336 EEC 2002 95 EC ROHS 2002 96 EC WEEE Standard s to which conformity is declared EN61010 1 EN55014 EN55 104 EN55022 EN61000 3 2 EN61000 3 3 Manutfacturer s Name Labconco Corporation Manufacturer s Address 8811 Prospect Avenue Kansas City MO 64132 USA Importer s Name See Shipping Customs Documents Importer s Address See Shipping Customs Documents for your equipment Type of Equipment Laboratory Equipment Sample Preparation Model No Stoppering Tray Dryer 230V 7948030 Triad Freeze Dry Tray Dryer 230V 7400030 Serial No Various See Individual Declaration Year of Manufacture 2004 and Subsequent I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s See individual Declaration of Conformity
10. Temperature Range C 55 to 50 Shelf Size Inches 12 4 x 14 5 x 5 75 high Number of Shelves l Defrost Method Hot Gas Time to Defrost 2 0L Hr 1 5 All Temperature Specification 20 C Ambient 48 Product Service 816 333 8811 or 1 800 522 7658 Appendix D Freeze Dry System Accessories The following accessories are available for the Triad Freeze Dry System PART DESCRIPTION Cable Connect the RS232 output from the Triad Freeze Dry System to an IBM compatible computer with a 9 pin serial data port Cable 7537801 Connects the RS232 output from the Triad Freeze Dry System to an IBM compatible computer with a 25 pin serial data port Tray with Slide Out Bottom 7756100 14 wide x 12 deep Stainless steel tray has separate bottom which slides out allowing serum bottles and ampules direct contact with the shelf to facilitate the drying process Bulk Tray 7156200 15 wide x 11 1 2 deep Stainless steel tray will contain liquids for bulk drying Support Grid 7 wide x 7 deep Stainless steel tray with removable plastic grid 7516200 provides support for stoppering ampules and other small specimen containers while stoppering under vacuum Grid holds 144 ampules of 12 mm diameter Vacuum Pump 7739403 Two stage direct drive pump 195 liters minute 230 VAC 50 60 Hz single phase 4 0 amps Includes Pump Exhaust Filter 1473400 Vacuum Pump 7439200 Two stage direct drive pump 173 liters minute 230 VAC 50 60 Hz single p
11. back panel of the Freeze Dry System Refer to Appendix D Triad Freeze Dry System Accessories for vacuum pumps available from Labconco 6 Product Service 816 333 8811 or 1 800 522 7658 Chapter 3 Getting Started Now that the site for your Triad Freeze Dry System 1s properly prepared you are ready to unpack inspect install and test your freeze dry system Read this chapter to learn how to e Unpack and move your freeze dry system e Setup your freeze dry system e Safely use solvents with your freeze dry system Unpacking Your Triad Freeze Dry System Carefully unpack your freeze dry system and inspect it for damage that may have occurred in transit If your freeze dry system is damaged notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier The Triad Freeze Dry System weighs over 400 Ibs 181 Kg The carton allows for lifting with a mechanical lift truck or hand truck If you must lift the freeze dry system manually use at least four 4 persons and follow safe lifting guidelines If your freeze dry system was damaged in transit you must file a claim directly with the freight carrier The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen 15 days of delivery Labconco Corporation and its dealers are not responsible for shipping damage Do not return goods without the prior authorization of Labconco
12. which will be signed by the importer for your country Place Signature Date Full Name Position Labconco P N 3696010 REV E ECO E077 Product Service 816 333 8811 or 1 800 522 7658 55
13. 0 300 or 600 seconds intervals e Press ENTER 16 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Operating Instructions The display will show VACUUM UNITS MBar Pa e At any time the DISPLAY button can be pressed to select a different function Setting the Operating Vacuum Level The vacuum level may be manually set by the user to optimize the freeze dry process Normally the sublimation rate will increase if there is less vacuum a higher pressure in the Triad Freeze Dry System A good starting place is to set the vacuum to a level that will hold the sample temperature approximately 10 C cooler than the eutectic temperature Adjustments to the vacuum level must be made for various freeze drying conditions Factors that must be considered are whether the sample is freeze dried on heated shelves or in glassware attached to manifold valves the volatility of the sample itself the size of the sample and the heat energy supplied to the sample When the vacuum control is set to operate at less vacuum the ice holding capacity of the collector may be decreased Some guidelines for setting the Triad Freeze Dry System are shown below These show pre freezing temperature and vacuum levels The user of the specific samples that are being freeze dried must determine exact protocols Temperature Temperatures Product Service 816 333 8811 or 1 800 522 7658 17 Chapter 4 Operating Instructions Manual Control Operat
14. 510 125 ml 7574210 7576210 7577110 7577510 Product Service 816 333 8811 or 1 800 522 7658 Appendix D Freeze Dry System Accessories Fast Freeze Flasks Variety of adapters available You may select from glass or stainless steel adapters available straight or with 45 bend Required order separately Compatible with all major brands of laboratory freeze dry apparatus No complex filter filters they are easily inserted between the adapter and top of the flask One hun dred filters are sup plied with each flask Top seals easily The flexible silicone rubber top snaps on and off easily yet provides a reliable high vacuum The complete Fast Freeze Flask has only two components a high strength borosilicate glass bottom to withstand extreme temperatures and high vacuum a flexible non contaminating silicone rubber top No vacuum grease is Wide mouth opening loads fast cleans easily Horizontal shell freezing Flask may be placed in a horizontal posi tion and rotated in a bath for shell freezing Fast Freeze Flasks are specially designed to be easier to handle faster to load and more convenient to use than other freeze dry glassware now in your laboratory Compatible with all major brands of laboratory freeze dry equipment Fast Freeze Flasks eliminate the risk of contamination from vacuum grease reduce spillage of valuable samples and require no washers gaske
15. 658 Chapter 5 Maintaining Your Freeze Dry System Monthly l The rubber components on the freeze dry system may eventually deteriorate and require replacement The effective life of rubber parts depends upon both their usage and the surrounding environment Check all rubber hoses and gaskets and replace any that show signs of hardening permanent set or deterioration Using a soft cloth sponge or chamois and a mild non abrasive soap or detergent clean the acrylic door Using a soft cloth sponge or chamois and a mild non abrasive soap or detergent clean the exterior surfaces of the cabinet Liquid spray cleaners and polishes may be used on the exterior surfaces Do not use solvents to remove stains from the exterior surfaces as they may damage the finish Annually 1 Every 12 months or more often if the freeze dry system is operated in a dusty environment the refrigeration system condenser should be cleaned Using a vacuum cleaner with brush attachment clean the condenser to ensure proper airflow for peak performance Product Service 816 333 8811 or 1 800 522 7658 31 Chapter 6 Using the RS232 Receptacle The operation of the Triad Freeze Dry System can be monitored using a computer connected to the RS232 receptacle located on the rear panel of the freeze dryer The computer cannot control the operation of the freeze dry system Computer Connection for Computer Interface Check your computer to see which type of
16. 7557800 103mm x 90 2mm 750 ml 7550900 7554900 7552400 7556400 7559400 7557900 145mm x 90 2mm 1000 ml 7551000 7555000 7552400 7556400 7559400 7558000 187mm x 90 2mm Valves on the freeze dry system drying chambers and manifolds offered in this catalog fit adapters of both 1 2 and 3 4 sizes Size information is provided for selecting adapters for older Labconco models and other freeze dryers Product Service 816 333 8811 or 1 800 522 7658 53 Appendix D Freeze Dry System Accessories Ampules Z Labconco Ampules are fabricated of highest quality borosilicate glass for strength and durability They are available in five different configurations to provide the ideal ampule for your specific lyophilization requirements from tray drying to flame sealing applications Ampules Ampules are supplied in packages of 100 paai Oxygen Natural Gas Sealing Torch 7578500 Torch specifically designed for flame sealing freeze dry ampules Seals all types of heat resistant glass Connects to natural gas butane or propane and oxygen with 1 4 ID hose connectors Shipping weight 3 Ibs 1 4 kg Ampule Valve Adapters 7593401 Adapter connects ampules to valve ports or stainless steel stems Ten per package Shipping weight 0 5 Ibs Stoppers 7572510 Stopper for Stoppering Ampules 7572310 100 per package Shipping weight 3 Ibs 1 4 kg 3 Pre Scored Round Size Straight
17. 9 2mm 145mm x 59 2mm 135mm x 90 2mm 190mm x 90 2mm 240mm x 90 2mm 380mm x 90 2mm Valves on the freeze dry system drying chambers and manifolds offered in this catalog fit adapters of both 2 and 3 4 sizes Size information is provided for selecting adapters for older Labconco models and other freeze dryers 7544810 Replacement Filter Paper 1000 10 packages of 100 1 diameter 28mm Pore size 17 micron Shipping weight 0 51b Product Service 816 333 8811 or 1 800 522 7658 Appendix D Freeze Dry System Accessories Lyph Lock Flasks Borosilicate glass adapters with either 19 38 or 24 40 standard taper joints have 90 bend Compatible with all major brands of labo ratory freeze dry apparatus Required order separately Only three pieces per flask no hooks or springs The complete Lyph Lock Flask includes e a flask top of high strength borosilicate glass The flexible ring holds securely together during pre freezing e a flexible non and lyophiliza contaminating silicone rubber seal e a flask bottom of borosilicate glass to with stand extreme temperatures and high vacuum Lyph Lock Flasks simplify your Lyophilization procedures because they have only three pieces per flask a high strength borosilicate glass top and bottom with a silicone rubber ring seal The unique wide
18. C System Vacuum Level is above 5 mBar During the ramp cycle of the active segment the status display will be as follows Pl Selected Program Pl SHLF 55 SAMP 55 C SHLF Shelf Temperature COL 85 SEG IR VAC 03M SAMP Sample Temperature if probe is not installed the display shows For C Temperature Units COL Collector Temperature SEG Shows what segment is active and Ramping R VAC System vacuum level M MBar P Pascal T Torr Product Service 816 333 8811 or 1 800 522 7658 21 Chapter 4 Operating Instructions During the hold cycle of the active segment the display will alternate as follows Pl Selected Program SHLF Shelf Temperature SAMP Sample Temperature if probe is not P1 SHLF 55 SAMP 55 C installed the display shows COL 85 SEG 1H VAC 030M COL Collector Temperature SEG Shows what segment is active and holding H TR Shows time remaining segment If time is P1 SHLF 55 SAMP 55 C 10 0 hours or more the time is displayed in COL 85 TR 4 6 VAC 030M 1 10 hours If time is less than 10 0 hours the time is displayed in 1 100 hours If the hold time was programmed to be indefinite the display will show INDF VAC System vacuum level M MBar P Pascal T Torr Changing the Program Parameters in the Middle of a Run 1 Press the DISPLAY button to obtain Monitor screen Note the current operating segment 2 Press the DISPLAY button to obtain Auto screen
19. ER The display will show VACUUM SET POINT XXXX YYY Where YYY is the units selected above e Press ff or until the desired vacuum operating level is displayed See Setting the Operating Vacuum Level for guidelines e Press ENTER The display will show TEMPERATURE UNITS AG S e Press ff or until the desired units are flashing e Press ENTER The display will show REFRIG TOTAL HOUR XXXX SERVICE HOUR XXXX Product Service 816 333 8811 or 1 800 522 7658 15 Chapter 4 Operating Instructions e Press ENTER if you do not want to reset SERVICE HOURS e Press and hold 4 for 5 seconds to reset the Service Hours to 0 This allows you to keep track of the time the refrigeration system operated since it was serviced The display will show REFRIGERATION TOTAL HOURS XXXX RESET SERVICE HOUR X e Press ENTER The display will show VACUUM TOTAL HOURS XXXX SERVICE HOUR XXXX e Press ENTER if you do not want to reset SERVICE HOURS e Press and hold 4 for 5 seconds reset the Service Hours to 0 This allows you to keep track of the time the vacuum pump operated since it was serviced The display will show VACUUM TOTAL HOURS XXXX RESET SERVICE HOUR e Press ENTER The display will show RS 232 TRANSMISSION RATE 10 SECONDS e Press tt or until the desired time interval is shown The time between data transmission may be set to occur at 10 30 6
20. IMENSIONS APPENDIX C FREEZE DRY SYSTEM SPECIFICATIONS Electrical Specifications Environmental Conditions Performance Specifications APPENDIX D FREEZE DRY SYSTEM ACCESSORIES DECLARATION OF CONFORMITY 44 46 47 47 48 49 55 Chapter 1 Introduction Congratulations on your purchase of a Labconco FreeZone Triad Freeze Dry System which 1s designed for laboratory lyophilization procedures The refrigerant used in the refrigeration system is CFC free so it will not endanger the environment The unit is easy to install and maintain Proper care and maintenance of this product will result in many years of dependable Service Freeze Dry Process Freeze drying is an important process in sample preparation and for the preservation and storage of biologicals pharmaceuticals and foods Of the various methods of dehydration freeze drying lyophilization 1s especially suited for substances that are heat sensitive Other than food processing e g coffee whole dinners freeze drying has been extensively used in the development of pharmaceuticals e g antibiotics and preservation of biologicals e g proteins plasma viruses and cell lines The nondestructive nature of this process has been demonstrated by the retention of viability in freeze dried viruses and microorganisms Freeze drying is a process whereby water or other solvent is removed from frozen material by converting the frozen water directly into vapor wi
21. KNOB amp STEM O ASSEMBLY SS 7 Diagnostic Mode In the event that there is a problem with the Triad Freeze Dry System a diagnostic mode is available This mode can be used to diagnose problems by isolating individual system components to confirm their operation With the unit Power Switch OFF press the button and turn the Power Switch ON The display will show DIAGNOSTIC MODE Press The display will show LEXSOL PUMP ON The Lexsol pump will turn on or press ff The display will show LEXSOL AND VAC PUMP ON The Lexsol pump vacuum pump and vacuum valve will turn on or press The display will show REFRIGERATION SHELF Product Service 816 333 8811 or 1 800 522 7658 37 Chapter 7 Troubleshooting The shelf valve opens and refrigeration system turns on or press The display will show REFRIGERATION COLL The collector valve opens The shelf valve closes refrigeration system remains on or press The display will show REF LEX PUMP The refrigeration system remains on The Lexsol pump turns on or press Tr The display will show DEFROST The defrost valve opens or press The display will show VACUUM PUMP The refrigeration system turns off The vacuum pump turns on or press ff The display will show VAC VENT VALVE The vacuum pump turns off The vacuum control valve closes or press ft The display will show SHELF TILT VALVE The vacuum control valve o
22. Trap 7509600 Sample Valve Kit Includes neoprene valve body knob and installation parts 50 Product Service 816 333 8811 or 1 800 522 7658 Appendix D Freeze Dry System Accessories Serum Bottles and Vials Threaded Vials Stoppers and threaded vials with Screw Caps are supplied in packages of 200 Size Vials with Stoppers Screw Caps Perfect for long term storage of freeze 5 ml 7762300 7762200 10 ml 7762600 7762200 dried samples Labconco Serum Bottles and Treaded Vials are specifically designed for lyophilization applications Their uniform thin wall construction ensures even freezing and drying Bottles and vials are ideal containers for use in the FreeZone Stoppering Tray Dryer Serum bottles also connect to valve ports on drying chambers and manifolds Serum Bottles Serum Bottles Stoppers and seals are supplied in packages of 100 Seal Crimper Secures tear away Aluminum Seals 7578000 Seal Crimper for 13 mm corkage Shipping weight 3 Ibs 1 4 kg 7578100 Seal Crimper for 20 mm corkage Shipping weight 3 Ibs 1 4 kg 20 mm 13 mm Split Aluminum _ Sleeve Type Size Corkage Corkage Stoppers Seals Stoppers 2 ml 7575010 7576010 7577010 3 ml 7575210 7576010 7577010 5 ml 7573010 7576210 7577110 757710 10 ml 7573210 7576210 7577110 75771510 20 ml 7573410 7576210 7577110 7571510 30 ml 7573610 7576210 7577110 7577510 50 ml 7573810 7576210 7577110 7577510 100 ml 7574010 7576210 7577110 7577
23. User s Manual FreeZone Triad Freeze Dry System Models 7400030 7400040 To receive important product updates complete your product registration card online at register labconco com Protecting your MI W __ aboratory environment Labconco Corporation 8811 Prospect Avenue Kansas City MO 64132 2696 800 821 5525 816 333 8811 FAX 816 363 0130 E MAIL labconco labconco com HOME PAGE www labconco com Please read the User s Manual before operating the equipment Copyright 2005 2007 Labconco Corporation All rights reserved The information contained in this manual and the accompanying products are copyrighted and Labconco Corporation reserves all rights Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change Warranty Labconco provides a warranty on all parts and factory workmanship The warranty includes areas of defective material and workmanship provided such defect results from normal and proper use of the equipment The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco whichever is sooner except the following e Purifier Delta Series Biological Safety Cabinets and PuriCare Lab Animal Research Stations carry a three year warranty from date of installation or four years from date of shipment from Labconco whichever is sooner
24. arts will be voided if maintenance has been obviously neglected If you have questions about using specific compounds in the freeze dry system contact Labconco Technical Service at 1 800 821 5525 or 816 333 8811 or e mail labconco labconco com Solvent Safety Precautions Ay WARNING Solvents used in the freeze dry system may be flammable or hazardous to your health Use extreme caution and keep sources of ignition away from the solvents When using flammable or hazardous solvents the vacuum pump must be vented to a fume hood Hazardous materials such as strong acids or bases radioactive substances and volatile organics must be handled carefully and promptly cleaned up if spilled If a sample is spilled in the collector chamber it must immediately be cleaned up per MSDS procedures or damage to the equipment or injury may occur 10 Product Service 816 333 8811 or 1 800 522 7658 Chapter 3 Getting Started NOTE Various Federal State or local regulations may govern the disposal of substances used in connection with this equipment All users of this equipment are urged to become familiar with any regulations that apply in the user s area concerning the dumping of waste materials in or upon water land or air and to comply with such regulations Product Service 816 333 8811 or 1 800 522 7658 11 Chapter 4 Operating Instructions Read this chapter to learn how to e Understand the display e Operate the controls e Connect samp
25. ck the electrical connections of the freeze dry system to the power source and then check the electrical connection of the vacuum pump to the freeze dry system If the vacuum pump has a power switch make sure that it is turned on If the vacuum is not adequate when the vacuum pump is operating proceed with the following steps 1 Check the oil and ensure it is clear and clean If the oil looks cloudy or has any particulates replace the pump oil If the oil 1s excessively dirty it may be necessary to flush the pump with clean oil several times To flush the pump run the pump 5 to 10 minutes to allow the oil to warm up Drain the oil and refill with clean oil Repeat as necessary 2 Check the oil level in the pump Ensure it is filled to the correct level Check vacuum hose connections from the pump to the freeze dry system and try running the unit Product Service 816 333 8811 or 1 800 522 7658 35 Chapter 7 Troubleshooting If vacuum problems continue consider obtaining a second vacuum gauge capable of reading a vacuum of 0 010 mBar It is often useful in determining if the vacuum pump is operating properly and the vacuum sensor reading 1s accurate Isolate the pump by disconnecting the vacuum hose from the freeze dry system Deadhead the pump by inserting the vacuum sensor from a secondary vacuum gauge into the end of the vacuum hose and observe the vacuum reading obtained Confirm that the pump 1s capable of achieving an ultimate
26. d to run too long sample may be lost The shelf temperature will cool to approximately 75 C and freeze the sample Note Starting the system at room temperature will take approximately 6 hours to freeze a 2 liter tray of water ie When the sample is frozen change the shelf Set Point Temperature to the desired temperature between 55 C and 50 C Turn on vacuum Shelf temperatures and vacuum set points can be changed at anytime during the manual freeze dry process Auto Mode with Pre Frozen Samples l With the display set to Auto program the desired ramp temperature and hold times for each segment of the program The PREFREEZE segment time should be set to 0 This allows the program to start at segment 1 Set mode to AUTO Start the refrigeration system by pressing RUN STOP Start the vacuum pump When the collector temperature reaches 80 C and the shelf temperature reaches the desired set point of segment 1 turn off the vacuum pump and release the vacuum Place the frozen samples on the shelf Close the door and turn the vacuum on Auto Mode with Unfrozen Samples 24 l With the display set to Auto program the desired ramp temperature and hold times for each segment of the program The PREFREEZE segment time must be set between 3 hours and INDEF This allows the shelf to cool and pre freeze the sample The samples must be completely frozen during PRE FREEZE or samp
27. e diaphragm and allow the platen to return to the raised position Determine the cause of the tilt condition before proceeding Vacuum Break Backfilling To open the chamber door the vacuum must be released To release the vacuum move the Vacuum Release Control to the OPEN position and shut off the vacuum pump by pressing the VAC button The vacuum can also be released more quickly by opening one of the sample valves Air enters the vacuum chamber through the Back Fill Port When the sound of air through the Back Fill Port is no longer audible the chamber door is ready to open To backfill the chamber with a gas connect a cylinder of the desired gas to the Back Fill Port The port will accept 1 8 tubing The bottle must be equipped with a regulator set to 15 psi maximum The gas can be metered with the Vacuum Release Control and chamber pressure monitored with the freeze dry system s vacuum gauge Allow the vacuum pump to run for a few minutes while the gas enters to permit the back fill gas to surround the freeze dried sample When the vacuum pump is turned off air will enter the system through the vacuum break valve located between the vacuum pump and the collector chamber Product Service 816 333 8811 or 1 800 522 7658 25 Chapter 4 Operating Instructions Freeze Drying Using Manifold Valves The following procedure should be followed when using the sample valves for the freeze dry process 1 Turn on the Triad Freeze D
28. er Should backfilling with an inert gas be required connect the gas supply line to the vent port on the valve before turning the plastic knob on the valve to vent position The sample container may now be removed In the vent position the bevel on the valve knob should point away from the sample port Ampules may be flame sealed while connected to a valve by using a sealing torch Care must be taken not to burn the valve An insulation material placed between the valve and the torch is recommended Defrosting After the freeze dry process is complete the ice that has accumulated on the collector coil must be removed The Triad Freeze Dry System is equipped with a rapid defrost feature The following procedure should be followed when defrosting the collector coil l Press the DEFROST button on the control panel The defrost indicator above that button will illuminate Allow unit to operate in this condition until all condensate is defrosted from the collector coil Turn the system off by pressing RUN STOP button or it will automatically shut off after 3 hours Place the drain hose in a suitable container to collect the condensation Remove the drain plug from the drain hose Replace drain plug and remove drain pan to dispose of the ice and wipe dry Press the DEFROST button the indicator will be off Wipe any excess liquid from inside of vacuum chamber replace drain pan CAUTION Utilization of acid requires i
29. ess is recommended 2 Product Service 816 333 8811 or 1 800 522 7658 Chapter 1 Introduction The rate of freeze drying is directly proportional to the vapor pressure and the vapor pressure is dependent upon both eutectic temperature and solute concentration of the sample For example a solution of sodium chloride and water would freeze dry at a slower rate than pure water The eutectic temperature of a sodium chloride solution 1s about 21 C and at this temperature the vapor pressure is about 1 16 that of water at 0 C Although the eutectic temperature is not dependent upon the concentration of sodium chloride the vapor pressure of the water would decrease as the concentration of sodium chloride increased This is due to the fact that as the solute concentration increases less of the surface area of the frozen sample is occupied by water In general most solutions or biological samples will have a eutectic temperature of 10 to 25 C However if the sample contains a simple sugar such as glucose or 1f the sample is animal or plant tissue the eutectic temperature may be as low as 30 to 50 C Freeze Dry Capacity The volume of a sample that can be freeze dried at one time is related to factors discussed previously and the size and design of the freeze dry system With any given instrument the capacity is based on the surface area of the sample the eutectic temperature and concentration of the sample and the rate and amo
30. fety Precautions CHAPTER 4 OPERATING INSTRUCTION Triad Freeze Dry Controls Operation Checklist Vacuum Pump Ballast Setting Operating the Triad Freeze Dry System Set Up Setting the Operating Vacuum Level Manual Control Operation Automatic Control Operation Programming Starting the Freeze Dry Cycle Changing the Program Parameters in the Middle of a Run Stopping the Freeze Dry Cycle Monitoring the system Operation Sample Freezing Shelf Loading Na nN WWN Ree OOO WW WB gt N _ Freeze Drying inside the Chamber Manual Mode with Pre Frozen Samples Manual Mode with Unfrozen Samples Auto Mode with Pre Frozen Samples Auto Mode with Unfrozen Samples Stoppering Vials Vacuum Break Backfilling Freeze Drying using Manifold Valves Defrosting Alarms System Temperature Variations Collector Temperature Variations Vacuum Vacuum Pump Service Shelf Temperature Set Point Power Failure Platen Tilt CHAPTER 5 MAINTAINING YOUR FREEZE DRY SYSTEM CHAPTER 6 USING THE RS232 RECEPTACLE Computer Connection for Computer Interface CHAPTER 7 TROUBLESHOOTING Vacuum System Vacuum Pump Gaskets Tubing Connections Sample Valves Diagnostic Mode Refrigeration Module Operation Filling the Fluid System Heat Transfer Fluid Precautions Heat Transfer Fluid First Aid Procedures CHAPTER 8 MODIFYING YOUR FREEZE DRY SYSTEM DISPLAY Modifying the Display APPENDIX A FREEZE DRY SYSTEM COMPONENTS APPENDIX B FREEZE DRY SYSTEM D
31. hase 2 9 amps Includes Pump Exhaust Filter 7670400 Vacuum Pump Corrosion Resistant 7769800 Two stage direct drive pump 173 liters minute 230 VAC 50 60 Hz single phase 2 9 amps Includes Pump Exhaust Filter 7670400 Product Service 816 333 8811 or 1 800 522 7658 49 Appendix D Freeze Dry System Accessories Pump Inlet Filter 1472200 Disposable filter that prevents oil back streaming and protects vacuum pump from sub micron particles Fits Vacuum Pump 7739403 Pump Exhaust Filter 1473400 Disposable filter that removes visible oil mist and odor from vacuum pump exhaust Fits Vacuum Pump 7739403 Replacement Element Oil Mist Pump Exhaust Filter Fits Pump Exhaust Filter 1473400 Fits Pump Exhaust Filter 1473400 or Vacuum Pump 7739403 Replacement Filter Element 7670400 Disposable filter that removes oil mist from the vacuum pump exhaust Fits Vacuum Pumps 7439200 and 7769800 Vacuum Pump Oil 1 Liter 1988000 A molecularly distilled hydrocarbon oil with low vapor pressure For Vacuum Pump 7739403 Vacuum Pump Oil 1 Liter A mineral oil with low vapor pressure For Vacuum Pumps 7439200 and 7769800 Soda Acid Trap Secondary trap that prevents migration of corrosive chemicals into vacuum pump 7772100 Replacement Media for Soda Acid Trap Carbon Solvent Trap 7772500 Secondary trap that prevents migration of organic solvents into vacuum pump 7772600 Replacement Activated Carbon Media for Carbon Solvent
32. ion The manual operation has three selectable modes Pre Freeze In Flask and controlled shelf temperature The Pre Freeze setting allows the shelf temperature to freeze samples below 55 C obtaining Max Cold temperature of approximately 75 C The In Flask setting is used to freeze dry pre frozen samples when using only the sample valves on the left side of the unit There is no shelf temperature control in this mode When using the controlled shelf set point temperature setting the shelf temperature will maintain the set point selected between 55 C to 50 C This mode should be used when freeze drying samples on the shelf A flask can also be added to the sample valves in this mode 1 Turn the Power Switch ON The display will become active 2 Push the DISPLAY button until the MANUAL indicator lights This will cause the manual display screen to be shown with the previously entered set point temperature or operational mode SET POINT TEMP 30 C The temperature units are displayed in the units F or C selected in the Set Up The temperature may be set to control the shelf anywhere from 50 C to 55 C in 1 C increments To pre freeze samples below 55 C set temperature to Pre Freeze To freeze dry samples using the manifold sample valves change set point temperature to In flask 3 If the set point or operating mode needs to be changed press the INCREASE or DECREASE button until the des
33. ire that claims be filed with the delivery carrier within fifteen 15 days of delivery Limitation of Liability The disposal and or emission of substances used in connection with this equipment may be governed by various federal state or local regulations All users of this equipment are required to become familiar with any regulations that apply in the user s area concerning the dumping of waste materials in or upon water land or air and to comply with such regulations Labconco Corporation is held harmless with respect to user s compliance with such regulations Contacting Labconco Corporation If you have questions that are not addressed in this manual or if you need technical assistance contact Labconco s Customer Service Department or Labconco s Product Service Department at 1 800 821 5525 or 1 816 333 8811 between the hours of 7 00 a m and 6 00 p m Central Standard Time Part 7373300 Rev B ECO E077 TABLE OF CONTENTS CHAPTER 1 INTRODUCTION Freeze Dry Process Freeze Dry Rates Freeze Dry Capacity Samples Containing Volatile Substances CHAPTER 2 PREREQUISITES Electrical Requirements Location Requirements Vacuum Pump Requirements CHAPTER 3 GETTING STARTED Unpacking Your Triad Freeze Dry Triad Freeze Dry Components Setting Up Your Triad Freeze Dry System Component Orientation and Hose Connection Venting the Vacuum Pump Electrical Connection Chemical Resistance of Freeze Dryer Components Solvent Sa
34. ired set point temperature or mode is displayed 4 Press ENTER If ENTER 1s not pressed within 10 seconds the set point will revert to the previously entered parameter 5 Press MODE button until the manual indicator lights 6 Press RUN STOP button The RUN STOP indicator will light and the refrigeration system and or heater will operate 7 If vacuum is required press VAC Button Do not run vacuum with unfrozen samples in chamber 8 To change the set point temperature in the middle of a run repeat steps 2 3 and 4 The system will adjust to the new temperature set point 9 To monitor system operation press DISPLAY until the Monitor indicator lights The display will simultaneously show shelf SHLF temperature collector COL temperature and the sample SAMP temperature If the sample probe is not plugged into its jack the SAMP temperature display will show If in the Pre freeze mode the COL display will show If in the In flask mode the SHLF the display will show 18 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Operating Instructions The segment numbers will always show M while operating in the manual mode Segment numbers are used only in the automatic mode to identify portions of the programmed cycle Temperatures and vacuum are displayed in the units selected during the set up procedure Above 5 0 mBar the display will show HIGH vacuum SHLF Shelf
35. lace the opposite end of the tubing into the fluid reservoir Fill the reservoir slowly allowing the fluid to drain through the system When the system appears full prepare to start the circulation pump Open the fluid circulation valve by completely down seating turn top valve stem completely clockwise to direct fluid to by pass through tubing to fluid reservoir Connect the unit to the proper power source Activate the pump by moving the Power Switch to the ON position while holding down the button To start the diagnostic display press the button The fluid system will circulate with the fluid going through the add on tubing purging itself of air Add fluid when needed to keep fluid in the reservoir After the fluid stream is established tilt the entire freeze dry system up and down Observe the fluid stream and repeat the tilt procedure if it 1s not free of bubbles Fill reservoir to level indicated by the label Turn power switch to the OFF position and disconnect power cord from supply outlet Close up seat fluid circulation valve turn valve stem completely counter clockwise closing the fluid tubing by pass Remove the tubing Replace the caps on the valve and replace insulation and covers Replace the cap on the tank Replace insulation and seal air tight Replace rear cover Product Service 816 333 8811 or 1 800 522 7658 Chapter 7 Troubleshooting Heat Transfer Fluid Precautions The fluid s
36. les Do not use the freeze dry system in a manner not specified by the manufacturer refer to Appendix C Freeze Dry System Specifications The electrical protection may be impaired if used inappropriately 12 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Operating Instructions Triad Freeze Dry System Controls The control panel for the Triad Freeze Dry System is shown with a description about its function E SETUP CD omo L NEXCOINGO MAN AO a v 0 13 a CACAL ALARM RUN 0 EMTER ACK DEFROE Figure 2 1 Display Displays all necessary programming and operational data Provides prompts to aid in programming 2 Display Button Changes format of display from monitor to auto to manual to set up screen 3 Display Indicators Green INDICATOR shows which display screen is shown Alarm Indicator Red INDICATOR shows that a system alarm has occurred Defrost Used to manually start or stop the defrost operation Vacuum Used to manually start or stop the vacuum pump Decrease Button Used in programming to decrease a parameter set point Increase Button Used in programming to increase a parameter set point ee et ON A E Enter Button Used in programming to enter a selected set point into memory 10 Mode Selector Button Selects the mode of operation either manual or automatic 11 Mode Indicator Green INDICATOR shows whether the control is set t
37. les will be compromised and damage could occur to the vacuum pump when turned on in segment 1 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Operating Instructions 2 Set mode to AUTO 3 Place the unfrozen samples on the shelf and close the door 4 Start the refrigeration system by pressing RUN STOP Note The vacuum pump will run 10 seconds and then turn off automatically Stoppering Vials The stoppering operation when desired is performed after the freeze dry process is complete and before breaking vacuum To seal vials move the Stoppering control toward the LOWER position This action allows the diaphragm above the stoppering platen to inflate causing the platen to lower The platen will lower until it contacts the vials on the shelf The rubber stoppers will be pressed into the vials Monitor the stoppering process by looking through the chamber door When all of the vials appear to be stoppered move the Stoppering Control to the RAISE position This opens the diaphragm to the vacuum pump which deflates the diaphragm Opening the Vacuum Release Control also deflates the stoppering diaphragm The Stoppering Control should be left in the RAISE position when not stoppering The stoppering mechanism is equipped with anti tilt switch which will stop the platen from lowering 1f it tilts more that 4 If this happens return the stoppering control valve to RAISE position This will deflate th
38. mmediate cleaning and neutralization after defrost or physical damage to the collector chamber and coil will result Do not attempt to chip ice from the collector coil as damage may occur Never attempt to start the vacuum pump when there is liquid in the collector chamber This could result in damage to the vacuum pump Product Service 816 333 8811 or 1 800 522 7658 27 Chapter 4 Operating Instructions Alarms A number of events may occur during a lyophilization procedure that can adversely effect the operation of the Triad Freeze Dry System If an event occurs the alarm indicator will flash and beep The beep will automatically mute itself after one minute The specific alarm can be identified by observing the display The following out of specification conditions will initiate an alarm Shelf Temperature Variations Once the shelf temperature has stabilized for 20 minutes if the manual set point temperature or automatic hold temperature varies more than 2 C as measured by the shelf temperature sensor the Alarm Indicator and the word SHLF on the display will flash until the end of the run Collector Temperature Variations If the temperature raises above 40 C and stays there for more than 20 minutes the Alarm Indicator and the word COL on the display will flash until the end of the run Vacuum Once the system vacuum is low and stabilized at a point where it changes less than 0 020 mBar in 5 min
39. mouth design helps you load samples easily and lyophilize efficiently How to select Lyph Lock Flasks for your Freeze Dry System Available in seven convenient sizes Entire flask may be autoclaved Provides a high vacuum seal for efficient lyophilization Select Lyph Lock Flasks fast based on your sample sizes Flasks should be filled no more than one third of their volume so that maximum surface area is achieved and efficient lyophilization is Adapters Add the adapters for connecting the Lyph Lock Flasks to the valve ports on your drying chamber or manifold 90 Bend Description assured A complete Lyph Lock Flask Adapter includes a glass top and bottom and a FE CI eee ie ce rubber ring seal Tops bottoms and 756999 Connects 19 38 STj Flask Top to seals are available separately as 3 4 valve Connects 24 40 STj Flask Top to 4 replacement components Adapters are 7568400 vet required to attach flasks to freeze dry 7563600 Connects 24 40 STj Flask Top to 3 4 1 x valve ports A SSS Complete Complete Dimensions Flask Size Flask Flask cane ps Aa pals ee ee Flask Bottom 19 38 STj 24 40 STj J J H x ID 25 ml 7550000 7550000 7552000 7556000 7559000 7557000 37mm x 34mm 50 ml 7550200 7554200 7552000 7556000 7559000 7557200 67mm x 34mm 100 ml 7550400 7554400 7552200 7556200 7559200 7557400 50mm x 59 2mm 250 ml 7550600 7554600 7552200 7556200 7559200 7557600 110mm x 59 2mm 500 ml 7550800 7554800 7552400 7556400 7559400
40. name and manufacturer are Name Lexol 542 Manufacturer Santa Barbara Chemical Co 927 Indio Muerto Santa Barbara CA 93140 805 963 7793 4 amp WARNING The fluid used for heat transfer in this system is combustible and hazardous Leaks and spills should be attended to immediately or serious injury could occur Handling and Storage Precautions Dirty Solvent Store in accordance with all applicable regulations Tighten caps and store in a cool area Precautions if Material is Released or Spilled Spills should be contained immediately Spills may be soaked up with absorbent materials placed in closed containers labeled stored and disposed of properly Persons performing this work should wear adequate personal protective equipment and clothing Disposal of Non Recyclable Solvents Dispose of in accordance with all federal state and local health and pollution regulations Follow same guidelines as when disposing kerosene Heat Transfer Fluid First Aid Procedures Eye Contact Immediately flush eyes with fresh water for at least 15 minutes If irritation persists get medical attention Skin Contact Wash contaminated areas with soap and water Remove contaminated clothing and footwear Wash clothing before reuse Discard footwear which cannot be decontaminated Medical attention may be required Inhalation Remove patient to fresh air If breathing stops give artificial respiration Get medical attention immediately 1f
41. ndix C Freeze Dry System Specifications This Appendix contains technical information about the freeze dry system s electrical specifications and environmental operating conditions Electrical Specifications Catalog Number 7400030 7400040 Voltage Operating Description Nominal Range Frequency Phase Amperage Triad Freeze Dry System 230 198 254 50 1 12 Triad Freeze Dry System 230 187 253 60 1 12 Environmental Conditions Indoor use only Maximum altitude 6562 feet 2000 meters Ambient temperature range 41 to 104 F 5 to 40 C Maximum relative humidity 80 for temperatures up to 88 F 31 C decreasing linearly to 50 relative humidity at 104 F 40 C Transient over voltages according to Installation Categories I Over voltage Categories per IEC 1010 Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed Used in an environment of Pollution degrees 2 1 e where normally only non conductive atmospheres are present Occasionally however a temporary conductivity caused by condensation must be expected in accordance with IEC 664 Product Service 816 333 8811 or 1 800 522 7658 47 Appendix C Freeze Dry System Specifications Performance Specifications Collector Minimum Temperature C 85 Collector Capacity in 24 Hours Liters 1 84 Collector Total Capacity Liters 25 Shelf Minimum Temperature C 75 Shelf Control
42. ntire surface of the shelf When stoppering vials always place a vial on each corner of the shelf Freeze Drying Inside the Chamber The following procedure should be followed when freeze drying using the temperature controlled shelf inside the vacuum chamber Manual Mode with Pre Frozen Samples 1 With the display set to manual select the desired set point temperature between 55 C and 50 C 2 Select MAN mode Start the refrigeration system by pressing RUN STOP Close the door turn on the vacuum 4 When the collector temperature reaches 80 C and the shelf temperature reaches the desired set point Turn off the vacuum pump and release the vacuum Place the frozen samples on the shelf Close the door and turn the vacuum on Product Service 816 333 8811 or 1 800 522 7658 23 Chapter 4 Operating Instructions Note The system will go from room temperature start up to 55 C in under 7 hours 5 Shelf temperatures and vacuum set points can be changed at anytime during the manual Freeze Dry process Manual Mode with Unfrozen Samples l With the display set in manual select Set Point Temperature Pre Freeze Set mode to MAN 3 Place unfrozen samples on the shelf and close the door The sample probe can be placed in the sample Start the refrigeration system by pressing RUN STOP Start the vacuum pump by pressing VAC Let the pump run 10 seconds Ifthe pump is allowe
43. o operate either in the automatic or manual mode Product Service 816 333 8811 or 1 800 522 7658 13 Chapter 4 Operating Instructions 12 Run Stop Button Initiates the start or stop of the lyophilization process 13 Run Stop Indicator Green INDICATOR burns steadily while freeze drying is in progress and turns off when the Run Stop button terminates a run The green INDICATOR flashes if freeze drying is in progress and a power failure occurs 14 Stoppering Controls the stoppering platen up and down movement The mechanism will function only while the chamber is under vacuum The stoppering action should be closely monitored when operating this control 15 Vacuum Release Vents the chamber so the chamber door can be opened This control can also vent gas into the chamber when the gas supply 1s properly connected to the backfill port 16 Back Fill A regulated tank of gas may be connected to the port to allow the introduction of gas into the chamber after freeze drying The port accepts 1 8 tubing CAUTION Ethylene Oxide is not recommended for use in this freeze dry system for decontamination because of its hazardous and corrosive properties 17 Power Switch Turns all power to Triad Freeze Dry System ON or OFF Not shown Located on left side of cabinet Operation Checklist The following checklist should be followed prior to each use of your freeze dry system 1 Wipe out the interior of the chamber with a soft cl
44. oth or paper towel to remove any moisture or debris 2 Check the collector tray drain hose to ensure that the hose is free of moisture and that the drain plug is securely installed 3 Using a soft lint free cloth or paper towel wipe the door gasket to remove any dirt and contaminants that could cause a vacuum leak Vacuum grease is not required on the door gasket 4 Check that each sample valve is closed or in the vent position See Figure 4 Vacuum Pump Ballast Setting Most vacuum pumps are equipped with gas ballast mechanism The freeze dry process requires high vacuum Therefore it is recommended that the gas ballast be closed during the operation of the Triad Freeze Dry System Note If the gas ballast is left open for extended periods of operation the oil can be pumped out the exhaust causing the pump to fail 14 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Operating Instructions Operating the Triad Freeze Dry System Set Up Vacuum may be displayed in mBar Pascal Pa or Torr and the temperature may be displayed as either F or C The refrigeration system and vacuum pump operating time may be monitored and the operating vacuum level may be set To configure your Triad Freeze Dry System turn the main Power Switch ON Press the DISPLAY button until the SET UP indicator is lit The display will show VACUUM UNITS MBar Pa e Press ff or until the desired units are flashing e Press ENT
45. pens The shelf tilt valve turns on open or press T The display will show HEATER ON The heater turns on for 3 minutes or press The display will go to monitor screen 38 Product Service 816 333 8811 or 1 800 522 7658 Chapter 7 Troubleshooting Refrigeration Module Operation In the Pre freeze mode under a no load condition and an ambient temperature of 20 C the freeze dry system refrigeration system can achieve a shelf temperature of 75 C or lower within 4 hours If any repairs are required on the refrigeration module please call Labconco Repairs should only be undertaken by a competent refrigeration technician or through an authorized Labconco service agency Filling the Fluid System Figure 6 FLUID RESERVOIR FLEXIBLE TUBE CAP FLUID CIRCULATION VALVE p a JUCTION BOX FLUID RESERVOIR Product Service 816 333 8811 or 1 800 522 7658 39 Chapter 7 Troubleshooting 1 2 3 4 5 6 7 8 9 10 11 12 13 40 Disconnect power cord from supply outlet Make sure the Power Switch is in the OFF position Remove the rear cover to expose the marked back foam insulation Cut through the foam along the marked lines and remove center cutout Remove the insulation and both caps from the fluid circulation valve Attach a flexible tube to the exposed port service port The port 1s 3 8 x 45 male flare Remove the fluid reservoir cap and p
46. required Ingestion Get medical attention immediately Do not induce vomiting Product Service 816 333 8811 or 1 800 522 7658 41 Chapter 8 Modifying Your Freeze Dry System Display Your Triad Freeze Dry System has been carefully calibrated and tested before shipping however under certain circumstances it may be necessary to adjust the calibration of the vacuum display or to reset factory settings These adjustments may be necessary if the freeze dry system requires service An offset may be added to the vacuum display Use an accurate traceable reference to compare the readings Vacuum offset may be varied 0 010 mBar in 0 001 mBar increments Modifying the Display To enter an offset or to restore all factory settings perform the following Steps e Press the DISPLAY button until the SET UP INDICATOR 1s lit e Press and hold DISPLAY button for 10 seconds The display will first show the screen used for setting Auto mode parameters and then will show PRODUCTION MENU If you want to restore all factory settings hold ENTER approximately 5 seconds This will reset Vacuum units to mBar Vacuum offset to 0 mBar Temperature units to C Refrigeration service hours to 0 Vacuum total hour to 0 Vacuum service hour to 0 Refrigeration total hours to 0 RS232 transmission rate to 10 seconds At the completion of Master Reset the display will return to the monitor mode 42 Product Service 816 333 8811 or 1 800 522 7658
47. ry System Select manual mode change the set point temp to IN FLASK and turn on the vacuum 2 Once the collector temperature is less than 80 C and vacuum is less than 0 133 mBar connect a pre frozen sample to a sample valve on the left side of the cabinet using an adapter Turn the plastic valve knob to the VACUUM position to open the valve The bevel on the knob should be positioned toward the sample port to apply vacuum to the sample see Figure 4 3 Before adding another sample allow system vacuum to return to the vacuum set point Any combination of valves and sample sizes may be utilized at one time provided that the system vacuum and collector temperature remain sufficiently low to prevent melting of the frozen sample 4 When all the frost has disappeared from the outer surface of the sample container and no cold spots can be detected by handling the container primary drying is nearly complete To be certain of low final moisture content dry the sample for several hours past this point Figure 4 ROTATE VALVE 180 TO APPLY VACUUM VENT PORT TO SAMPLE GAS SUPPLY FOR BACK FILLING SAMPLE VALVE SHOWN IN CLOSED POSITION PRE FROZEN SAMPLE PALL LLL 26 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Operating Instructions To remove a container after freeze drying is complete turn the plastic knob on the valve to the VENT position which closes the valve and vents the contain
48. serial port 1s provided Use one of the two connecting cables listed below 1 Computers with a 25 pin D sub male serial connector should use Connect Cable Labconco part number 7537801 to connect a computer to the Labconco Freeze Dry System 2 Computers with a 9 pin D sub male serial connector should use Connect Cable Labconco part number 7537800 to connect a computer to the Labconco Freeze Dry System The purpose of the RS232 interface 1s to send data to a data logging computer to monitor the state and activity of the freeze dry system This data is half duplex data The data properties are as follows Data Rate 2400 Baud 8 bit word length 1 Start bit 1 Stop bit No parity is transmitted Standard ASCII character set ge Cae ue The time between data transmissions may be varied by the user to occur at 10 30 60 300 or 600 second intervals Press the display button until the set up indicator lights Press the enter button until the RS232 screen appears on the display RS 232 TRANSMISSION RATE 10 SECONDS 32 Product Service 816 333 8811 or 1 800 522 7658 Chapter 6 Using the RS232 Receptacle Press 7 or amp until the desired time interval is shown Press ENTER The format of the transmitted message with typical values is as follows PROG 1 SEG 2R TRM 17 COL 85 SHLF 55 SAMP 55 VAC 180 Where PROG Program 1 Program is selected M Manual Mode S Stop SEG Segment 2 Segment 2 is operating R
49. t Service 816 333 8811 or 1 800 522 7658 Chapter 4 Operating Instructions The display will show the parameters that were last entered for this program PI SEG 1 RAMP 00 0 C MN HOLD 34 C TIME 00 0 If any of the parameters are changed the ENTER button must be pressed for the new value to be stored If ENTER is not pressed within 10 seconds after the last parameter adjustment the value will return to the previously stored value The flashing parameter name shows the parameter that will be changed Pressing ENTER only will advance to the next parameter After the AUTO RUN cycle is started segments with a value greater than the segment running may have their parameters changed Only the selected program may be modified To modify a different program the system must be stopped and the desired program selected Starting the Freeze Dry Cycle After the desired cycles have been programmed push the MODE button to select AUTO Push the RUN STOP button to start the program running The RUN STOP indicator will light The system display will change and the display will change to MONITOR mode During the pre freeze segment the status display will be as follows 3 Pl Selected Program P1 SHLF 70 SAMP 70 C SHLF Shelf Temperature COL SEG PR VAC hi SAMP Sample Temperature if probe is not installed the display shows For C Temperature Units COL No temperature displayed SEG Pre Freeze Mode R Ramp H Hold VA
50. tem requires little maintenance However the following maintenance schedule 1s recommended As needed I 30 The user has the responsibility for carrying out appropriate decontamination 1f hazardous material is spilled on or inside the equipment This may be done by wiping the contaminated surfaces with a soft cloth dampened with alcohol Alcohol may craze the acrylic door Before using any cleaning or decontamination method except those recommended by Labconco users should check with Labconco to determine that the proposed method will not damage the equipment Clean up all spills remove liquids from the chamber Clean door and gasket using soft cloth sponge or chamois and a mild non abrasive soap or detergent Check oil level of the vacuum pump It should be between MIN and MAX If the oil level is less than an inch 25 4 mm above MIN add oil to proper level If oil shows cloudiness particles or discoloration drain the pump and replace with fresh oil The use of acids requires immediate cleaning and neutralization after a run or physical damage will result When freeze drying biological substances it may be necessary to decontaminate the system A surface decontaminant should be used to clean the accessible surfaces CAUTION The use of ethylene oxide is not recommended because of its hazardous and corrosive nature Contact Labconco for additional information Product Service 816 333 8811 or 1 800 522 7
51. temperature SAMP Sample probe if probe is not installed the display shows ge C F F COL Collector Temperature SEG Shows what segment is active and ramping or holding M Manual mode VAC System vacuum level m mBar P Pascal T Torr 10 To stop push RUN STOP button which will turn off the refrigeration system and the heater To turn off the entire system turn the Power Switch OFF Automatic Control Operation The Triad Freeze Dry System is equipped with a microprocessor based controller that permits temperature to be programmed using as many as six different segments The first segment is Pre freeze with automatic Max Cold setting and an adjustable time setting of 0 3 hours to 99 9 hours or indefinite The additional five segments consist of a temperature ramp function and a temperature hold function There are five programs of six segments each that can store settings One of the five may be selected by pressing the DISPLAY button until the AUTO indicator lights Press the UP DOWN buttons to select the desired program and then press ENTER The display will change to show the parameters for this program The ramp allows the temperature of the sample to be increased or decreased at any desired rate within the capacity of the heating and cooling systems of the Triad Freeze Dry System The Triad Freeze Dry System without samples on shelves is capable of cooling at a rate of approximately 5 C minute from 50 C to
52. the collector temperature shelf temperature system vacuum and sample temperatures If the sample probe is not connected the sample temperature display will show The temperatures and vacuum are displayed in the units selected in Set Up Above 5 0 mBar the display will show hi vacuum The ice collecting coil temperature and the vacuum level should be monitored Higher than desired collector coil temperature or vacuum levels will inhibit or ruin the freeze dry process Sample Freezing Before the freeze dry process can occur the products to be dried must be in a frozen state This can be accomplished in a freezer separate from the freeze dry system or on the shelf in the Triad Freeze Dry System If the samples will be pre frozen on the shelf inside the Triad turn ON the Power Switch and set the controls to cool the shelf This is done by setting the temperature to pre freeze in the manual mode or the pre freeze segment in the automatic mode can be programmed to cool the shelves and start the vacuum after the end of the pre freeze segment If desired place the temperature probe in a sample vial Monitor the shelf and sample temperatures by pressing the DISPLAY button until the monitor indicator lights Shelf Loading The stoppering mechanism is capable of generating a very strong force which can damage the shelf or mechanism Therefore it 1s important to distribute the serum bottles to be stoppered evenly across the e
53. the new connection Press OK 4 The Connect To dialogue box will open Using the down arrow selection button select the communication port to which the cable has been connected Press OK 5 The Com X Properties dialogue box will open Enter the appropriate data properties and press OK 6 When the freeze dry system main power switch is on the data will be a a as dc al ahead taeda transmitted and updated at the time S SEG E 18 9999 COL 40 SHLF 39 SAHP 99 VRC 9999 gt intervals selected by the user Stee aati aca ee cette aoe sE TRH 9999 COL 40 SHLF 39 SAMP 39 VAC 9999 gt E TRH 9999 COL 40 SHLF 39 SAMP 39 VAC 9999 gt E TRH 9999 COL 40 SHLF 39 SAHP 39 VAC 9999 gt 34 Product Service 816 333 8811 or 1 800 522 7658 Chapter 7 Troubleshooting Refer to the following if your freeze dry system fails to operate properly If the suggested corrective actions do not solve your problem contact Labconco for additional assistance Vacuum System FreeZone Triad Freeze Dry Systems that are clean and dry and without samples attached should reach a vacuum of 0 133 mBar within 30 minutes and should achieve an ultimate vacuum of 0 040 mBar within 18 hours when the refrigeration is operating If the freeze dry system does not obtain a satisfactory vacuum perform the following maintenance tests Vacuum Pump First make sure that the vacuum pump operates If it fails to operate che
54. the rate of freeze drying This is based on the fact that the heat of sublimation is usually absorbed on one side of the frozen sample and must travel through the frozen layer to vaporize water at the other surface In addition as the sample is freeze dried the water vapor must travel through the layer of dried material The thicker the sample the greater the chance that the dried layer may collapse which would cause an additional decrease in the rate of freeze drying The surface area and thickness of the sample can usually be ignored when each sample contains only a few milliliters However for larger volumes the samples should be shell frozen to maximize the surface area and minimize the thickness of the sample The volume of the freeze dry container should be two to three times the volume of the sample In order for lyophilization to occur ice must be removed from the frozen sample via sublimation The collector and the vacuum pump accomplish this The collector which should be at least 15 to 20 C colder than the eutectic temperature melting temperature of the sample traps vapor as ice Since the vapor pressure at the collector 1s lower than that of the sample the flow of water vapor 1s from the sample to the collector Since this vapor diffusion process occurs very slowly under normal atmospheric conditions a good vacuum is essential to maintain an efficient rate In many applications the maintenance of a vacuum of 0 133 mBar or l
55. thout the intermediate formation of liquid water The basis for this sublimation process involves the absorption of heat by the frozen sample in order to vaporize the ice the use of a vacuum pump to enhance the removal of water vapor from the surface of the sample the transfer of water vapor to a collector and the removal of heat by the collector in order to condense the water vapor In essence the freeze dry process 1s a balance between the heat absorbed by the sample to vaporize the ice and the heat removed from the collector to convert the water vapor into ice Product Service 816 333 8811 or 1 800 522 7658 1 Chapter 1 Introduction Freeze Dry Rates The efficiency of the freeze drying process is dependent upon the surface area and the thickness of the sample the collector temperature and vacuum obtained the eutectic point and solute concentration of the sample It is important to remember these factors when trying to obtain efficient utilization of your freeze dry system A listing of selected materials and their approximate drying times are shown in Table for your reference Table 1 Thick ae cme nae eae a Mik Urea a H o 0 Total sample quantities are contingent on various Triad Freeze Dry System capacities Up to the point of overloading the system the greater the surface area of the sample and the faster the rate of freeze drying By contrast for a given surface area the thicker the sample the slower
56. ts or retainers Available in nine convenient sizes How to select Fast Freeze Flasks for your Freeze Dry System Select fast Freeze Flasks based on your sample sizes Flasks should be filled no more than one third of their volume so that maximum surface area is achieved and efficient lyophilization is assured A complete Fast Freeze Flask includes a rubber top glass bottom and a supply of filter paper Tops bottoms and filter paper are available separately as replacement components Adapters are required to attach flasks to freeze dry valve ports Flask Size 40 ml 80 ml 120 ml 150 ml 300 ml 600 ml 900 ml 1200 ml 2000 ml Complete Flask 7540000 7540200 7540300 7540400 7540600 7540800 7540900 7541000 7541200 Flask Bottom 7542000 7542200 7542300 7542400 7542600 7542800 7542900 7543000 7543200 Flask Top 7544000 7544000 7544200 7544200 7544200 7544400 7544400 7544400 7544400 Flask Top Adapter Diameter VAM yy ot 34 34 34 34 34 34 necessary No threads hooks or springs are needed Entire flask may be autoclaved Flat bottom reduces spills provides better balance Adapters Add the adapters for connecting the Fast Freeze Flasks to the valve ports on your drying chamber or manifold Choose borosilicate glass or stainless steel adapters in 2 and 3 4 diameters Dimensions Flask Bottom HxD 76mm 34mm 115mm x 34mm 68mm x 59 2mm 85mm x 5
57. unt of heat transferred to the frozen sample Of these factors the eutectic temperature is the most important factor in determining the amount of sample that can be freeze dried at one time particularly when flasks are used This is because as the eutectic temperature decreases the vapor pressure decreases but the rate of heat absorption by the sample does not change This tends to promote melting of the sample which leads to a marked increase in vapor pressure and ultimately overloads the collector and vacuum pump Samples that have eutectic temperatures of 20 C or lower should be placed on the freeze dry system one flask at a time so that the vacuum in the system may recover before adding another sample to the system If the vacuum does not recover the capacity of the freeze dry system has been exceeded and the sample should be removed If there is a problem with a particular type of sample melting when placed on the freeze dry system dilution of the sample with more water or providing some insulation around the flask to decrease the rate of heat absorption by the sample may help Samples Containing Volatile Substances In certain cases the solvent in a sample to be freeze dried may contain volatile components such as acetonitrile methanol acetic acid formic acid or pyridine Product Service 816 333 8811 or 1 800 522 7658 3 Chapter 1 Introduction In addition to these substances having an effect on the eutectic temperature the
58. ute and should achieve an ultimate vacuum of 0 040 mBar or lower within 18 S hours If 0 040 mBar cannot be achieved consult the troubleshooting section of this manual Chemical Resistance of Freeze Dry System Components The FreeZone Triad Freeze Dry System is designed to be chemically resistant to most compounds that are commonly used in Freeze drying processes However by necessity the freeze dry system 1s comprised of a number of different materials some of which may be attacked and degraded by certain chemicals The degree of degradation 1s dependent on the concentration and exposure duration Some of the major components of the freeze dry system that are susceptible to degradation are as follows Acids Buffers Solvents T D V V ga om os m lt T E o z V 2 sE 22 S82 g 2 6 o 0 22 S S 2 SA S55 FE SE PER BREE Component Material ae Pe OC Ck ee Se ee Valve Stem Acetal Delrin C D D D D Door Acrylic D D D D Hoses Gaskets amp Valve Neopren C D D C C DDD Bodies Flask Top Drain Hose Silicon Rubber C D D D D Chamber amp Fittings Stainless Steel C C Moderate degradation limited use D Severe degradation infrequent use recommended immediate thorough cleaning required Product Service 816 333 8811 or 1 800 522 7658 Methyl t Butyl Ether BTBE Pyridine Chapter 3 Getting Started e Most common compounds used in freeze drying processes if allowed to enter the vacuum pump
59. utes if the vacuum changes more than 0 500 mBar the Alarm Indicator and the word VAC on the display will flash until the end of the run Vacuum Pump Service When the vacuum pump service hours reaches 1000 hours The Alarm Indicator and the VAC Indicator will flash on for 3 seconds and off for 1 second When the vacuum is turned off the VAC Indicator will flash 1 second on and 3 seconds off Resetting the vacuum pump service hours will cancel the alarm Shelf Temperature Set Point If during a Ramp mode the system temperature stabilizes without reaching the set point temperature the control will enter the next Hold mode The Alarm Indicator will flash and the program indicator Px on the display will flash until the end of the run Power Failure If a power failure occurs while a run is in progress the Alarm Indicator and RUN STOP Indicator will flash when the power is restored Once power 1s restored the process will continue as programmed if the collector temperature has not risen above 30 C Pressing Run Stop cancels the flashing warning 28 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Operating Instructions Platen Tilt If the stoppering platen tilts more than 4 while stoppering the platen will stop The display will read SHELF UNEVENLY LOADED Product Service 816 333 8811 or 1 800 522 7658 29 Chapter 5 Maintaining Your Freeze Dry System Under normal operation the freeze dry sys
60. vacuum pump Secure with the clamp provided The hose may be cut to proper length to allow for gentle bends without kinks Connect the power cord from the vacuum pump into the outlet on the back of the unit If your vacuum pump power cord does not have a female IEC plug replace the cord end with the female IEC plug provided with the unit Part number 1291000 Figure 1 VACUUM HOSE CLAMP a VACUUM PUMP INLET FITTING Ka BY IEC VACUUM J POWER OUTLET VACUUM EXHAUST 8 Product Service 816 333 8811 or 1 800 522 7658 Chapter 3 Getting Started Venting the Vacuum Pump If any materials will be placed in the freeze dry system that can liberate hazardous gases when heated the vacuum pump exhaust must be vented to a fume hood or other ventilation device Electrical Connection Connect the power cord into the receptacle on the back of the freeze dry system and connect the other end into a suitable power receptacle The freeze dry system is now installed and must be tested to make certain the system is free of leaks Close the door turn on the freeze dry system refrigeration and allow the collector temperature to reach 40 or lower this could take 20 to 30 minutes Make sure the Stoppering Control is in the RAISED position and the Vacuum Release control is in the CLOSED position Start the vacuum pump and monitor the vacuum gauge The vacuum on the freeze dry system should reach 0 133 mBar within 30 min
61. vacuum less than 0 010 mBar or approximately 10 microns If an inadequate vacuum reading is obtained the pump has most likely failed and may need to be replaced or rebuilt Gaskets Tubing Connections and Sample Valves 1 Check all sample valves on the side of the cabinet and ensure all valves are closed or in the vent position 2 Check all vacuum tubing for signs of deterioration or cracking Check all connections and make sure they are secure and leak tight 4 Check the freeze dry system door gasket for indentations cracks or tears Clean gaskets using a soft lint free cloth or paper towel The freeze dry system chamber and collector must be dry 6 When checking the sample valves pull as much vacuum as possible 36 If a vacuum indication is displayed wiggle or rotate the valves and watch the gauge for any fluctuation Fluctuations can show a potential vacuum leak If the valve seems to be in good condition remove the valve and apply a thin coat of vacuum grease to the stem and the outside sealing surface of the valve body and reinstall the valve Ifthe valve still seems to be the source of the problem remove the valve and stopper the hole with a rubber stopper Continue checking the other valves Product Service 816 333 8811 or 1 800 522 7658 Chapter 7 Troubleshooting The illustration below shows how the sample valve installs on the chamber Figure 5 4 N SS S SS S S SS S S SS S SS S 4 i s
62. will degrade the oil and cause damage to the vacuum pump e Sugars and proteins typically will have minimal negative effect on any of the materials of construction When using compounds in the freeze dry system that are hostile to the materials of construction it is imperative the equipment is thoroughly cleaned after use e Rubber and plastic components that have been exposed to damaging compounds should be removed and flushed with water e The oil in the vacuum pump should be checked often It must be changed if it is cloudy shows particles or is discolored The useful life of vacuum pump oil can be extended if the vacuum pump is operated for an extended period of time after a freeze dry run This allows contaminants to be purged from the hot oil This must be done with the inlet to the pump blocked off to prevent air from free flowing through the pump This is accomplished by closing all sample valves on a clean dry freeze dry system and turning on the vacuum pump If the pump is operated at an elevated vacuum level gt 10mBar oil may be expelled from the pump and damage could occur Optional secondary traps are available to help extend the life of the vacuum pump These traps are installed between the freeze dry system and the vacuum pump Contact Labconco for assistance in determining which trap is right for your application With prudent maintenance the freeze dry system will provide years of service Warranty on the affected p
63. y may increase the vapor pressure at the surface of the sample Also compared to water they will require the absorption of less heat for sublimation to occur Hence samples that contain volatile substances will have a greater tendency to melt particularly when placed in flasks or exposed to room temperature If a sample containing a volatile substance tends to melt when placed on a freeze dry system dilution of the sample with more water will help keep the sample frozen For example a 0 2M solution of acetic acid is much easier to freeze dry than a 0 5M solution 4 Product Service 816 333 8811 or 1 800 522 7658 Chapter 2 Prerequisites Before you install your Triad Freeze Dry System you must be certain that the area is level and of solid construction An electrical source must be located near the installation site Carefully read this chapter to learn e Electrical supply requirements e Location requirements e Vacuum pump requirements Refer to Appendix C Freeze Dry System Specifications for complete electrical and environmental conditions specifications and requirements Electrical Requirements The Triad Freeze Dry System requires a dedicated electrical outlet This outlet requires a 20 Amp circuit breaker or fuse for all models The power cord on 230V models is equipped with a NEMA 6 20P plug If this does not match with the available receptacle remove this plug and replace it with an approved plug of the suitable style

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