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1. small concave front lens with a very small radius and slight curva ture The surface of this front lens can be readily cleaned with a toothpick covered with a cotton tip or with a small cotton swab Wipe the front lens lightly without applying undue force or scrubbing action Make sure that the cotton tip contacts the concave lens surface Check the objective with a magnifier after cleaning Leitz Optilux User Manual Care of the Microscope Dimensions Leitz Optilux User Manual Dimensions Dimensions e side view with monocular tube e front view with monocular tube e side view with monocular tube 180 rotated for storage Leitz Optilux User Manual Dimensions The productive cooperative effort with the user for the user has always been the basis for the innovative strength of Leica Microsystems On this we have developed our five corporate values pioneering high end quality team spirit dedication to science and continuous improvement We call making these values reality Living up to Life INDUSTRY DIVISION The Leica Microsystems Industry Division s focus is to support customers pursuit of the highest quality end result by providing the best and most innovative imaging systems for their needs to see measure and analyze microstructures Its solutions are used in routine and research industrial applications in materials science and quality control in forensic science investigations and educational applicatio
2. stand Work Surface Always use the microscope on a hard A stable surface Leitz Optilux User Manual Using the Leitz Optilux Selecting an Objective Using the Condenser Selecting an Objective 2 Rotate the condenser diaphragm below the The condenser is furnished with an iris 1 Rotate the desired objective over the specimen stage so that the displayed value diaphragm which has to be adjusted to sample matches the selected objective match the effective numerical aperture of the individual objectives _ Leitz Optilux User Manual Using the Leitz Optilux Preparing for Viewing Preparation 2 Insert the specimen slide and slowly release 3 Using the X Y control position the speci 1 Open the specimen holder using the the tension lever men slide such that a part of the specimen tension lever is under the objective used OPTILUX k Leitz Optilux User Manual Using the Leitz Optilux Focusing Focusing 1 Use the knurled ring to rotate the objective nosepiece in such a way that the objec tive with the lowest magnification level is turned into the working position OPTILUX T i TE Leitz Optilux User Manual Move the specimen stage upwards as far as it will go using the coarse adjustment knob Using the Leitz Optilux Look into the eyepieces and adjust the illu mination to a comfortable intensity Bring the specimen into focus using the fine adjustment knob Adjusting t
3. Living up to Life Jeica MICROSYSTEMS Leitz Optilux User Manual Congratulations Congratulations on purchasing the Leitz Optilux Compound Microscope This model s exclusive design features and full range of accessories make it a truly versatile high quality instru ment Though the reliability and robustness of Leica microscopes is already practically proverbial they still require a certain degree of care and attention Therefore we recommend that you read this User Manual It contains all the infor mation you need regarding operation safety and maintenance Simply observing a few guidelines will ensure that even after years of intensive use your microscope will continue to work as smoothly and reliably as on the very first day We wish you the best of success in your work Leitz Optilux User Manual 2 Contents Important Notes Using the Leitz Optilux Symbols Used in this User Manual 5 Switching on the Microscope 23 Important Safety Notes 6 Selecting an Objective 24 Instructions for Use 8 Preparing for Viewing 25 Health Risks and Dangers of Use 10 Focusing 26 Information for the Person Responsible for the Instrument 11 Adjusting the Eyecups 27 Accessories Maintenance and Repair 12 Oil Immersion Technique 28 Electrical Data and Ambient Conditions 13 Maintenance instructions 31 Unpacking 15 General Maintenance 32 Installing the Monocular Tube 17 Removing and Inserting Object
4. Manual is always avail able at the place where the microscope is in use Carry out regular inspections to make certain that the authorized users are adher ing to safety requirements When instructing new users do so thor oughly and explain the meanings of the warning signs and messages Allocate responsibilities for commission ing operation and maintenance Monitor compliance with this Do not use the Leitz Optilux unless it is in perfect condition Leitz Optilux User Manual Inform your Leica representative or Leica Microsystems Schweiz AG Industry Division 9435 Heerbrugg Switzerland immediately of any product defect that could potentially cause injury or harm If you use accessories from other manu facturers with the microscope make sure that these manufacturers confirm that the combination is safe to use Follow the instructions in the User Manual for those accessories Modifications to or service on the micro scope may be carried out only by techni cians who are explicitly authorized by Leica to do so Only original Leica replacement parts may be used in servicing the product Important Notes e After service work or technical modifica tions the device must be readjusted in accordance with our technical specifica tions If the unit is modified or serviced by unau thorized persons is improperly maintained as long as maintenance was not carried out by Leica Microsystems or is handled impro
5. amage to property Doing so can impair the functions of the protective measures provided The responsible Leica affiliate must be A consulted whenever the instrument is altered modified or used in conjunction with non Leica components that are outside of the scope of this User Manual Unauthorized altera tions to the instrument or noncompliant use shall void all rights to any warranty claims Important Notes Safety Concept Brochure The Safety Concept brochure contains addi tional safety information regarding service work requirements and the handling of the microscope the electrical and other accessories as well as general safety instructions maa un Bezpe nostn koncepce Sikkerhedskoncept Sicherheitskonzept Ohutuseeskiri Safety concept _ Concepto de seguridad Concept de s curit Concetto di sicurezza Dro ibas noteikumi Saugos samprata Biztons gi el ir sok Veiligheidsconcept Sikkerhetskonsept Koncepcja bezpiecze stwa Conceito de seguran a Bezpe nostn pokyny Varnostni koncept Turvallisuus S kerhetskoncept hica RES miens eus RETA Important Safety Notes continued Legal Regulations Adhere to general and local regulations relat ing to accident prevention and environmental protection EC Declaration of Conformity Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Decla ration of Conf
6. configuration e Optional items such as contrast accessories camera adapters cameras and carrying cases are not shipped as part of the stan dard equipment These items are delivered in separate packages e Please do not discard any of the packing materials They should be used for safely storing and transporting the instrument should the need arise Leitz Optilux User Manual Assembling the Leitz Optilux Identifying your Tube Orientation A All tubes are connected via a dovetail and can be installed in any orientation Monocular EZTube Leitz Optilux User Manual Assembling the Leitz Optilux Installing the Monocular Tube Tools used Assembly e Allen key 1 Loosen the setscrews on top of the stand using the Allen key provided TN 2 Insert the dovetail of the monocular tube into the mount Eyepieces The eyepieces are already integrated in a the Leica EZTube and preset for this reason the eyepieces do not require adjust ment or assembly Leitz Optilux User Manual Assembling the Leitz Optilux 3 Turn the tube in the direction you desire 4 Tighten the set screws on the left and right without applying too much force Removing and Inserting Objectives The objectives are already installed la at delivery The following description shows how to un install the objectives if they have to be removed for cleaning or replaced by other objectives Your Leitz Optilux is delivered with
7. e mount of the illu minator illuminator i The mirror is equipped with a flat and a concave surface Try them out to see which side works better 3 Look through the eyepieces and align the mirror so that it works best Leitz Optilux User Manual Assembling the Leitz Optilux Installing the Polarizer Kit optional The polarizer kit is not included in the Assembly delivery package but available as an 1 If you have already installed the tube optional accessory remove it see page 17 2 Place the analyzer with the flat side facing down into the beam path The two screws have to rest in the two existing holes 3 Install the tube Leitz Optilux User Manual Assembling the Leitz Optilux 4 Place the polarizer onto the aperture of the illuminator The handle must come to rest in the aperture with the white arrow point ing upwards 5 Turn the polarizer to align it The arrows indicate the current orientation Using the Leitz Optilux Switching on the Microscope For safety reasons the power cable Startup Procedure 3 Switch on the microscope using the toggle is grounded via the microscope base 1 Attach the power cable to the rear side of switch on the rear side of the stand Never use an adapter between the power cable the microscope and the power supply that would render the grounding ineffective 2 Set the illumination to the lowest setting using the controller on the bottom left of the
8. echnique continued 7 Look through the microscope and slowly rotate the fine adjustment knob so that the specimen stage moves upwards until the specimen is in focus 8 If you are finished w orking w ith t he o il immersion objective clean the front side of the objective the specimen slide and all other surfaces that have come into contact with the oil following the instructions in the Care of the Microscope section on page 30 Leitz Optilux User Manual Using the Leitz Optilux Care of the Microscope Maintenance instructions General Notes Protect the microscope from moisture fumes and acids and from alkaline caustic and corrosive materials Do not store chem icals in the vicinity Protect the microscope from oil and grease Never grease or oil mechanical parts or slid ing surfaces Follow the instructions of the disinfectant manufacturer We recommend concluding a service contract with Leica Service Leitz Optilux User Manual Cleaning coated Parts and plastic Parts Dust and dirt particles should be removed with a soft brush or lint free cotton cloth Remove coarse debris with a moistened disposable cloth Acetone xylene or nitro containing thin ners must NOT be used Never use chemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abraded particles Care of the Microscope General Maintena
9. he Eyecups If you wear eyeglasses for microscope la viewing keep the rubber eyecups folded down If you do not wear eyeglasses you may find it useful to unfold the rubber eyecups in order to help block out ambient room light Leitz Optilux User Manual Using the Leitz Optilux Potential Sources of Infection Direct contact with eyepieces is a poten A tial transmission method for bacterial and viral infections of the eye The risk can be minimized by the use of individual eyecups Eyecups can be ordered separately Please contact your Leica partner Oil Immersion Technique Oil Immersion Technique 3 Add a drop of immersion oil from Leica 5 Slowly move the specimen stage upwards 1 Search for the area on the specimen slide to the area of the specimen slide you are using the coarse adjustment knob until that you want to study studying the oil drop on the slide comes into direct contact with the lens of the oil immersion 2 Move the specimen stage down to the oar objective lowest position using the coarse adjust ment knob 4 Rotate the 100x objective to the working position 6 Hold the knurled ring on the objective nosepiece and swing the objective back and forth to eliminate air bubbles Then bring the oil objective into the final posi tion so that the oil drop is between the front lens of the objective and the speci men slide Leitz Optilux User Manual Using the Leitz Optilux Oil Immersion T
10. hich are located behind the power cord recep tacle Only use fuses of the following type 5x20 mm 1 0 A 250 V high breaking capacity 13RFAG30003 Electrical Data Input 100 240 V 50 60 Hz 5 W 3 W LED General safety notes This instrument of safety class 1 has been built and tested in accordance with the following safety requirements for electrical equipment for measu rement control and laboratory use EN 61010 1 2002 08 EN 55011 2007 A2 2010 05 EN 61010 2 2008 06 EN 60825 1 2008 05 IEC 61010 1 2010 06 IEC 60825 1 2007 03 EN 61326 1 2006 10 LED Class 1 EN 61326 2 6 2006 10 In order to maintain this condition and to ensure safe operation A the user must follow the instructions and warnings contained in this instruction manual Environment Temperature for use 10 C 40 C Storage temperature 20 C 52 C 50 F to 104 F Manipulation shock 25 mm on 50 mm hard wood Transport shock unpacked 100 g 6ms Transport shock packed 800 mm free fall Transport vibrations unpacked 5 200 Hz 1 5g Air pressure during use and storage 500 1060 mBar Humidity during use and storage 20 90 Installation Category II Overvoltage Category Pollution degree 2 Leitz Optilux User Manual Important Notes Assembling the Leitz Optilux Unpacking e Carefully remove the microscope and any components from the packaging e Check the components against the planned
11. ives 18 Ce Installing the Mirror optional 20 Dimensions 35 Installing the Polarizer Kit optional 21 Leitz Optilux User Manual 3 Important Notes Symbols Used in this User Manual Warning Safety hazard This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following e Hazards to personnel e Instrument malfunctions and damage Leitz Optilux User Manual Warning of hazardous electrical Voltage This symbol indicates information that must be read and observed Failure to comply can cause the following e Hazards to personnel e Instrument malfunctions and damage Important Notes Important Information This symbol indicates additional infor mation or explanations that are intended to provide clarity Important Safety Notes A Before installing operating or using the instrument it is mandatory to read this User Manual In particular please follow all safety instructions The instruments and accessories described in this User Manual have been tested for safety and potential hazards Original Condition To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in this User Manual Leitz Optilux User Manual Non intended Use Using the instrument in any way other A than that described in this User Manual may result in personal injury or d
12. nce General Always use both hands to carry the microscope Leitz Optilux User Manual Keep all optical components clean Clean liness is important for maintaining good optical performance When the microscope is not in use it should always be put away with protection against dust If any optical surface becomes coated with dust or dirt clean the surface by blowing it off with a syringe or brushing it off with a camel hair brush before attempting to wipe the surface clean Care of the Microscope e Optical surfaces should be cleaned using a lint free cloth with a commercially available glass cleaner Use solvent only very sparingly so that it does not flow out over the lens No part of the microscope is quite so vulnerable to collecting dirt dust and oil as the front lens of the objective Whenever you encounter lack of contrast cloudiness or poor defini tion carefully check the condition of the front lens with a magnifier General Maintenance continued e The lower magnification objectives have Illumination fairly large front lenses and can be cleaned e The Leitz Optilux uses an LED illuminator with a cloth or lens tissue wrapped around a Therefore no lamp changing is required for finger and moistened with window cleaner the entire life of the microscope e Cleaning 40x and 100x objectives requires more care To attain the typical flatness for objectives with higher magnification the objective has a
13. ns www leica microsystems com Leica Microsystems an international company with an experienced worldwide customer service network Internationally active Phone Fax Australia North Ryde 61 288703500 2 9878 1055 Belgium Diegem 32 27909850 2790 98 68 Denmark Ballerup 45 44540101 44540111 Germany Wetzlar 49 6441294000 64 41 29 4155 England Milton Keynes 44 800 298 2344 1908 246312 France Nanterre Cedex 33 811000 664 15605 23 23 Italy Milan 39 02 574 861 02 574 03392 Japan Tokyo 81 3 5421 2800 3 5421 2896 Canada Concord Ontario 1 800248 0123 847 405 0164 Korea Seoul 82 25146543 25146548 The Netherlands Rijswijk 31 70 4132 100 70 4132 109 Austria Vienna 43 1 486 80500 1 486 80 50 30 Portugal Lisbon 351 21 388 9112 21 385 4668 Sweden Kista 46 8625 45 45 8 625 45 10 Switzerland Heerbrugg 41 717263434 71 726 34 44 Singapore 65 6779 7823 6773 0628 Spain Barcelona 34 934949530 93 494 95 32 USA Buffalo Grove lllinois 1 800248 0123 847 405 0164 People s Republic of China Hong Kong 852 25646699 2564 4163 Shanghai 86 21 6387 6606 21 6387 6698 13IDE14060EN Copyright by Leica Microsystems Schweiz AG CH 9435 Heerbrugg 2014 Subject to modifications LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH eca MICROSYSTEMS
14. objec l tives with magnification levels 4x 10x and 40x possibly also with a 100x objective Otherwise this can be retrofitted at any time Leitz Optilux User Manual Always turn the objective nosepiece by its knurled ring Never turn the objective nosepiece by using an objective as a handle Removing Objectives 1 Unscrew the objectives to remove them Assembling the Leitz Optilux The proper Sequence When inserting the objectives you can begin with any available space on the microscope Continuation of the sequence however has to be selected such that the magnification always increases when turning clockwise Continued on next page Removing and Inserting Objectives continued Inserting Objectives 1 Screw the 4x objective into any available space on the objective nosepiece 2 Turn the objective nosepiece clockwise one unit 3 Screw in the next largest objective 4 Repeat steps 2 and 3 until all objectives have been installed Leitz Optilux User Manual Assembling the Leitz Optilux Installing the Mirror optional The mirror is available as an optional Installing the Mirror Removing the Mirror i accessory By means of this the ambient 1 Position the microscope at a well lit loca 1 Simply pull the mirror out of the holder light can be used for illuminating the sample if tion for example before a window no power source is available for the built in LED 2 Press the mirror onto th
15. of the user Depending on the duration of uninterrupted work at such a workplace impaired vision and problems with the musculoskeletal system may occur For this reason appropriate measures for reduction of the workload must be taken e Optimum workplace layout e Frequent changes of activity e Thorough training of the personnel giving consideration to ergonomic and organiza tional aspects The ergonomic optical design and construction of the Leica microscopes are intended to reduce the exertion of the user to a minimum Danger of Infection Direct contact with eyepieces can be a A potential transmission path for bacterial and viral infections of the eye il The risk can be minimized by the use of personal eyecups Dangers during Use e The Leitz Optilux may be connected to a grounded socket only e The Leitz Optilux may be used when it is in proper working condition only LED Class 1 LED 1 Laser Product The illumination meets laser class 1 require ments in accordance with IEC 60825 1 Safety of Laser devices Caution Hazardous radiation may occur if controls adjustment jigs or procedures other than those outlined in this User Manual are used Leitz Optilux User Manual Important Notes Information for the Person Responsible for the Instrument Information for the Person Responsible for the Instrument Ensure that the Leitz Optilux is used only by persons qualified to do so Ensure that this User
16. ormity Leitz Optilux User Manual Disconnecting from the Power Supply Always position the Leitz Optilux so that you can disconnect it from the power supply at any time The power cable must remain accessible at all times because the power cable is intended as a power discon nect device Plug A Never install any other plug NEMA 5 15P or unscrew any mechani cal components unless expressly instructed to do so in the instructions Important Notes Instructions for Use This User Manual describes the Leitz Optilux and includes important instructions related to operating safety maintenance and accessories Your Leitz Optilux includes an interactive CD ROM with all relevant User Manuals in additional languages Keep it in a safe place and readily accessible to the user You can also download User Manuals and updates from our website at www leica microsystems com Leitz Optilux User Manual Intended Use The Leitz Optilux is an optical instrument for improving the visibility of objects through magnification and illumination It is used for observing Place of Use The Leitz Optilux microscope may be used only in closed rooms and must be placed on a solid surface The Leitz Optilux can be used at 10 C to 40 C Protect the microscope from oil chemi cals and extreme humidity If it is used outdoors the microscope must be protected from dust and moisture Never use electrical instruments
17. outdoors and place them at least 10 cm away from the wall and from flammable substances Important Notes Avoid large temperature fluctuations direct sunlight and vibrations These conditions can distort measurements and micrographic images In warm and warm damp climatic zones li the individual components require special care in order to prevent the build up of fungus Instructions for Use continued Accessories from third party Suppliers You can combine individual system articles with articles from external suppliers Please read the User Manual and the safety instructions from the supplier Integration in third party products When installing Leica products into third party products the manufacturer of the complete system or its dealer is responsible for follow ing all applicable safety instructions laws and guidelines Leitz Optilux User Manual Transport If at all possible use the original packaging for shipping or transporting individual modules In order to prevent damage from vibrations all moving parts that according to the User Manual can be assembled and disassembled by the customer should be packed separately Disposal Disposal must comply with locally applicable laws and regulations Important Notes Health Risks and Dangers of Use Health Risks Workplaces with microscopes facili l tate and improve the viewing task but they also impose high demands on the eyes and hand muscles
18. perly Leica Microsystems will not accept any liability The building s electrical wiring must comply with the relevant national stan dard in other words we recommend using a current operated ELCB system RCB Accessories Maintenance and Repair Accessories Maintenance Repairs and Service Work Only the following accessories may be used e The Leitz Optilux microscope is basically e Only original Leica Microsystems spare with the Leitz Optilux maintenance free To ensure that it always parts may be used operates safely and reliably werecommend Before opening the instruments switch off e The Leica Microsystems accessories that you take the precaution of contacting the power and unplug the power cable described in this User Manual the responsible service organization e Avoid contact with powered electrical e Other accessories provided that these have We recommend concluding a service circuits which can lead to injury been expressly approved for use by Leica contract with Leica Microsystems Microsystems with regard to safety tech S rvice Address nology In case of problems please contact us as follows Contact address Leica Microsystems Schweiz AG Industry Division Max Schmidheiny Strasse 201 CH 9435 Heerbrugg Switzerland Leitz Optilux User Manual Important Notes Electrical Data and Ambient Conditions Fuse Replacement Unplug the instrument before changing any fuses The Leitz Opti lux has two fuses w
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